Forensic Science and Technology Commons^™

A Forensic Lens On Aces & Trauma, Greta K. Cruz

Master of Arts in Human Services

Adverse Childhood Experiences (ACEs) have emerged as a critical area of research in childhood development and the prevention of criminal behavior. The framework of this topic continually provides updated information on the impact of criminal behavior on children. Understanding the long-term effects of trauma on mental health, the influence of criminal behavior, and patterns of recidivism across the lifespan when children are exposed to a single form of adversity or multiple forms of adversity. This research aims to synthesize recent empirical data/findings of how specific ACEs, such as physical abuse, sexual abuse, exposure to familial violence, and household substance misuse, …

Method Development And Optimization For The Detection Of Synthetic Cannabinoids In Hair Using Lc-Msms, Linda K. Obeng

Student Theses

Synthetic cannabinoids represent one of the most prevalent and high-risk classes of New Psychoactive Substances (NPS), continuously evolving in structure and potency. Their widespread presence in seized materials and toxicology casework underscores the growing need for reliable analytical methods capable of detecting these compounds in biological matrices. Hair offers several advantages for drug testing, including a long detection window, minimal invasiveness, and resistance to adulteration; however, detecting synthetic cannabinoids in hair remains challenging due to their low incorporation levels and structural diversity. This study focuses on the method development and optimization of an LC-MS/MS technique for the detection of selected …

Fast & Forensic: Rapid Detection & Quantitation Of 30 + Emerging Novel Psychoactive Substances In Hair Using Lc-Ms/Ms, Seokjin Hwang

Student Theses

The continuous emergence of novel psychoactive substances (NPS) poses a significant challenge for forensic and clinical toxicologists, due to their structural diversity, rapid turnover on the illicit drug market, and limited toxicological information. Hair is a powerful matrix for retrospective drug exposure assessment and monitoring NPS worldwide. Not only does it provide a broad window of detection but also a simple sample exchange amongst laboratories. However, the extraction of multi-class NPS in a single method remains an analytical challenge. Therefore, there is an urgent need for extraction techniques and analytical methods that are both rapid and capable of multi-class quantitation …

Eyewitness Consent To Be Video Recorded: Implications For Identification Accuracy And Confidence, Sarah Argüelles

Student Theses

Recently, a recommendation has emerged for law enforcement to record the full eyewitness identification procedure, recognizing the critical role eyewitness testimony plays in the justice system (Wells et al., 2020). However, in some states, such as New York, law enforcement is required to seek consent from witnesses before recording (Municipal Police Training Council & Division of Criminal Justice Services, 2017). Since this practice is relatively new, there has been limited research on how obtaining consent may affect eyewitness accuracy. It was hypothesized that obtaining consent to video record could be linked to a witness’s confidence in their identification, with higher …

Investigating The Relationship Between Noun Classes And Plant Folk Taxonomy In Chasu Language Of Kilimanjaro Region In Tanzania, Peter Rabson Mziray

Journal of Humanities and Social Sciences

The current study investigates the relationship between noun classes and plant folk taxonomy in Chasu (G 22). The study focuses on two objectives: the first objective is to describe the plant folk taxonomy in Chasu and the second objective is to determine the relationship between noun classes and plant folk taxonomy in Chasu. Data were collected from rural villages in Same and Mwanga districts by using free listing, field interviews (jungle-walk-and-identify), and written texts containing Chasu plant names. The findings reveal that Chasu folk taxonomy reflects different ethnobotanical categories; including a unique beginner which is mmea/mimea ‘plant(s)’, and three life …

Debunking Myths: Sickle Cell Trait, Crises & Sudden Death, Michael R. Debaun, Corey J. Hebert, Yvette Marie Miller

School of Medicine Faculty Publications

No abstract provided.

Behavioral Assessment Ai Software: Enhancing Polygraph And Investigative Interviewing, Alan Saquella

Publications

In the rapidly evolving world of security, law enforcement, and investigative sciences, professionals are increasingly turning to advanced technologies to supplement tried-and-true methodologies. One emerging innovation is behavioral assessment software, designed to enhance both preemployment screening and investigative interviewing. Acting as a form of modern human intelligence collection, these tools enable examiners and investigators to gather deeper insights directly from candidates and subjects, revealing behavioral cues and risk indicators that might otherwise remain hidden (Vrij, 2008).

Effects Of Rapid Shearing Depending On Environmental Factors: Acrylic, Modacrylic, And Polypropylene Fibers, Charles K. Jin

Student Theses

Textiles can often be found damaged in forensic casework, and analysis of these defects can yield valuable information. Different methods of fiber fracture leave behind varying alterations in structure on individual fiber ends, leading to their possible identification and connection to a case. Interactions between high-speed impacts and synthetic fabrics composed of thermoplastic fibers are classified as rapid shear or high-speed tensile breaks. In these situations, unique fiber end characteristics can form due to excessive heat generation that is unable to dissipate quickly enough to prevent fiber end morphology from changing.

This study aimed to explore how different external conditions …

Exploring Variability Of The Cg18562578 Methylquant Assay For The Forensic Discrimination Of Monozygotic Twins, Sydney E. Arnold

Student Theses

Monozygotic (MZ) twins possess identical DNA sequences, posing a problem for forensic scientists, as they are incapable of distinguishing MZ twins from each other using traditional DNA identification methods. However, the epigenomes of MZ twins will differ and might be used for their forensic discrimination. Here, we explored the variability of an assay previously developed by our laboratory that showed high discrimination potential for MZ twins using buccal swab DNA, cg18562578 MethylQuant. We measured precision at each step: bisulfite conversion, nested PCR, and qPCR, reproducibility, and repeatability. DNA methylation ratios were compared between two analysts carrying each step using the …

Further Investigation Of Environmental Factors On Rapid Shear In Fibers Of A Polyester-Cotton Blend, Daniel K. Oh

Student Theses

Rapid shear is a process that occurs in thermoplastic materials when they interact with a high-velocity impact. In forensics, these interactions would typically be from a bullet interacting with clothing that consisted of synthetic fibers. When the interaction occurs, bulb-like shapes appear on damaged fiber ends, distinguishing them from other kinds of damage. The reason why these shapes occur is varied, but one prevailing factor seems to be the heat generated by the impact.

In prior experiments, the factor of temperature being important to the formation of the phenomenon, different environmental simulations that affected the sample’s temperature were done on …

Non-Destructive Dry Vacuum Recovery Of Dna From Fabric, Emily Sam-Zee-Moi

Student Theses

Trace DNA has proved to be a reliable forensic tool when investigating crimes. Current methods of trace DNA recovery from fabric include cutting, swabbing, scraping, and tape lifting. A dry vacuum collection method originally developed for paper evidence is an alternative nondestructive option for DNA collection from fabric. This research evaluated the success of the dry vacuum technique on pieces of cotton fabric that had been briefly touched as well as the effectiveness of different moistening agents and filters. The samples were designed to simulate an assault with the suspect grabbing the clothing of their victim. Volunteers created samples by …

The Effect Of Bullet Morphology And Intermediate Targets On Bullet Path, Natalia Aguilar

Student Theses

A key feature of shooting reconstruction is trajectory analysis. There are several different methods of assessing this from the bullet impact mark including: best-fit ellipse, lead-in, trajectory rod, 3D laser scanning, and machine learning. While these function in differently, many share a common fault. Few trajectory analysis techniques evaluate the difference in bullet path for bullet morphology. This raises the question: does the morphology of a bullet influence its degree of deviation when interacting with a target material? This study investigates the difference in bullet trajectories through drywall for 9mm full metal jacket (FMJ) and jacketed hollow point (JHP) ammunition …

The Normalization Of Clandestine Graves In Mexico, Alejandra Vela

Themis: Research Journal of Justice Studies and Forensic Science

Clandestine graves are increasing at an alarming rate in Mexico, as cartels fight for dominance over the wealthiest territories. Those who fall victim to cartels are brutally tortured into submission or are murdered and disposed of. These graves are the preferred method of body disposal by the cartels. By disposing of various victims in hidden locations, the cartels seek to erase the existence of these individuals. Victims are reduced to only bone fragments and ash. Friends and family of those who have gone missing have created a large search organization, Madres Buscadoras, to combat the helplessness of authorities. Madres Buscadoras …

Oviposition And Immature Development Of Blowflies In Forensic Investigations, Amalia Herrera-Benitez

Themis: Research Journal of Justice Studies and Forensic Science

Forensic entomology is a subspecialty within the field of forensic science where insect evidence is analyzed to solve criminal cases. Insects begin to colonize the body, with blowflies being the first insects to arrive. Within minutes, blowflies can lay eggs on a decomposing body, and the larvae will feed once they hatch. The life cycles of blowflies are analyzed to help determine an estimated post-mortem interval (PMI), which is crucial in investigations, as it can help establish a timeline. Various factors, such as temperature and access to the body, can cause delays in the oviposition of blowflies. A delay in …

The Rape Kit Backlog: Problems And Solutions, Tracey Cao

Themis: Research Journal of Justice Studies and Forensic Science

This literature review examines the ongoing issue of rape kit backlog in the United States. Rape kits are a means of evidence collection from victims of sexual assault that can be used in court. Across the nation, many state jurisdictions have hundreds and thousands of rape kits left untested and locked away in storage facilities. Consent for testing, police bias, and funding issues are the main issues that will be addressed. Many victims choose not to have their kits tested. Other kits never get tested due to police choosing not to submit a kit due to their bias against some …

Detection Canines: An Insight Into Investigative Working Dogs, Adriana Pliego

Themis: Research Journal of Justice Studies and Forensic Science

In a comprehensive exploration of detection canines, this paper aims to research the depth of their utilization by many investigative agencies and fields. A relevant case is discussed to present the application of detection canines and how they are useful for investigative measures. Shedding light into the various components of detection canines, this paper will discuss desirable canine characteristics that are sought out. On the other hand, undesirable characteristics are also discussed to inform recruiters of which attributes they should opt out of. This paper continues to discuss the roles and capabilities of detection canines given their unique potential. Then, …

Forensic Psychology Of Serial Killers, Karla Suarez

Themis: Research Journal of Justice Studies and Forensic Science

Many serial killers share patterns in psychological and neuropsychological disorders and behaviors. These behaviors and patterns should be recognized and acknowledged to understand why serial killers murder without remorse or guilt. It is important to discuss matters such as common patterns, typologies, and characteristics within serial killers. After looking at these essential aspects of serial killers, common neurodevelopmental disorders, brain damage, and biological epigenetics will be further explored. In order to fully assess this topic, forensic psychology and behavioral analysis are essential to aid in understanding serial killers and how their brains function to lead them to the actions that …

Comparative Analysis Of Forensic Science Systems: Scotland Vs. America, Alec Noland

Themis: Research Journal of Justice Studies and Forensic Science

Forensic science plays an important role in the justice system of both the United States and Scotland, but the structure, application, and effectiveness differ significantly. This research paper presents a comparative analysis of forensic science in two different legal and institutional contexts, examining differences between the two forensic science systems. This includes forensic laboratories and services, education and training, policing, court procedures, legal framework, and crime scene investigation. The analysis highlights significant differences such as Scotland’s centralized system versus America’s decentralized system, which impacts resource allocation and standardization for forensic science. Despite major differences, both countries emphasize scientific reliability, standardized …

What Modren Dental Dna Sequencing And Tooth Analysis Combined With Historical Context Can Tell Us About The Human Experience, Brynn Butko

D.U.Quark

Dental DNA extraction and visual analysis of teeth are subjects that aid many different research fields. Odontal analysis, specifically combined with forensic science and anthropology, allows for age and sex estimations, the identification of remains, and an in-depth historical account of human history. Because tooth analysis is utilized in various fields, a lack of uniform protocol for handling, analyzing, and extracting teeth persists. This review will explore the methods of dental analysis, the individual dental characteristics that identify age and sex in remains, and a depiction of the past through tooth evaluation. Creating a standardized protocol for the process of …

Deepfakes On Trial: Developing A High-Accuracy, Court-Admissible Ai Pipeline For Deepfake Detection In Corporate Fraud Litigation, Aiden J. Green

Honors College Theses

As deepfake technology advances, cybercriminals are increasingly using AI-generated videos and audios to impersonate executives and carry out sophisticated CEO fraud schemes. These synthetic forgeries target human trust and corporate communication systems, creating an urgent need for forensic tools capable of authenticating digital evidence with legal accuracy. This thesis presents a forensic-grade AI deepfake detection pipeline designed for this purpose, emphasizing courtroom admissibility, reproducibility, and evidentiary integrity. Built entirely with free, opensource tools, the framework combines metadata analysis, AI-powered spectrogram analysis, neural artifact detection, and facial manipulation recognition into a transparent workflow that accurately identifies synthetic media. It was trained …

Heat Induced Diagenesis And Osteometrics: An Experimental Approach To Assessing Thermal Alterations To Forensic Anthropological Data On Dry Bone, Audrey Holbeck

Department of Anthropology: Theses and Student Research

Fire appears in forensic casework in instances of arson, vehicle fire, house fire, to conceal evidence, or, in increasing contemporary frequency, wildfire, as a result of climate change. Thermal alteration is a destructive process to osseous material and can impact the accuracy of identification methods and trauma analysis by alteration of the structure, appearance, and morphology of human remains. This study seeks to validate previous research on the metric and morphological changes bone undergoes when exposed to extreme heat, and how that may impede estimation of metric features such as stature. Three long bones, a humerus, femur, and tibia, from …

Comparative Analysis Of Gunshot Trauma And Decomposition In Southeast Nebraska, Alexandra C. Tieken

Department of Anthropology: Theses and Student Research

Accurately identifying gunshot trauma (GST) in forensic anthropology remains a significant challenge, particularly in distinguishing the effects it has on decomposition. This study investigates how different types of gunshot trauma influence the rate and characteristics of decomposition in a humid continental climate, specifically southeastern Nebraska. Utilizing Sus scrofa domesticus (domestic pigs) as human proxies, four pigs were euthanized by professionals and subjected to controlled conditions at the Reller Prairie Field Station. Three pigs were inflicted postmortem with close-range gunshot trauma using a 9mm handgun, a 12-gauge shotgun, and an AR-15 rifle, while a fourth served as a trauma-free control. For …

Wildfire And Decomposition: A Taphonomic Analysis Of Thermal Alterations And Decomposition, Ashley Novak

Department of Anthropology: Theses and Student Research

Within the field of forensic anthropology, practitioners may be faced with remains that have been thermally altered by thermal alterations caused by wildfire. Within the field, very little research has been conducted examining how these thermal events impact rates of decomposition. While research has been conducted generally on the effects of thermal alteration on decomposition, these studies do not look through this lens and provide answers to the question of how do thermal alterations caused by wildfires affect rates of decomposition? This research examines the effects of thermal alteration caused by wildfire on the rate of decomposition. A decompositional study …

Firearm Trauma Across Time: Investigating Civil War Gunshot Trauma And Their Impact On Modern Forensic Analysis, Morgan K. Reeder

Department of Anthropology: Theses and Student Research

This thesis will examine the ballistic trauma caused by Civil War-era firearms and ammunition. These weapons, each with distinct characteristics, had a profound impact on the nature of combat and the resulting injuries. By investigating how these firearms’ ammunition interacted with human bone structure, this research will offer a detailed analysis of the trauma these weapons produced.

This research intends to answer several key research questions. First, how did the different types of Civil War firearms affect skeletal remains? Second, what are the key characteristics of gunshot trauma caused by these firearms in terms of bone fracturing and fragmentation, and …

Trends In Dna Aanalysis To Determine Postmortem Interval: A Systematic Lilterature Review, Victoria S. Kashkin

Department of Anthropology: Theses and Student Research

Forensic taphonomy is a recently popular field within forensic anthropology with limited research in the Central Great Plains. Given decomposition varies by region, more studies must be done to understand aspects of decomposition in this region. However, it is one of the most important subfields of forensic anthropology since determining postmortem interval (PMI) is extremely important to successfully identifying a missing individual if other indications of who the individual is are nonexistent. In this study, a systematic literature review was conducted to answer what trends are seen in research being done for using DNA to determine PMI, and what methods …

Influence Of Biotic And Abiotic Factors On Decomposition And The Determination Of Time Since Death: Applications Of Forensic Entomology And Taphonomy, John Oladapo Obafunwa

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The determination of the postmortem interval (PMI) is essential for the investigation and successful prosecution of suspected cases of homicide. While the forensic pathologist might be able to draw fairly accurate conclusions during the initial 24–36 hours, beyond this period, the assistance of other experts like forensic entomologists and taphonomists would be required. The present study was designed to observe the postmortem changes in Sus scrofa placed in a cage in Nebraska, located in the American Central Great Plains. The setting is in an open grassland in late spring to early summer. The observed variables with respect to biotic and …

Maggot Mass Effect On The Development And Survival Of Forensic Blow Flies, Akomavo Fabrice Gbenonsi

School of Natural Resources: Dissertations, Theses, and Student Research

Forensic entomology scientists use blow flies' developing patterns to estimate the postmortem interval (PMI). However, larval aggregations produce metabolic heat, resulting in complex microclimates that may change growth timetables. This study investigates how larval density and associated temperature changes affect the development and survival of two forensically essential blow fly species, Lucilia sericata and Calliphora vicina.

This study examined how larval density and associated thermal profiles affect the development of both blow fly species. Using a split-plot design, we tested four larval densities at 25°C and 30°C, with temperature gradients measured via thermocouple at four mass positions three times …

Bullet Ricochet And Tunnelling Of Wood Substrates, Abdelrahman Khalifa

Student Theses

With the overall rise in shooting incidents throughout the country, most notably in urban areas, the capabilities and limitations of current forensic examinations of firearms-related incidents have become more apparent. An underappreciated aspect of shooting incidents is the phenomenon of bullet ricochet, which has led to ricochet-related injuries, with some cases proving fatal. Although there are processes and generally accepted standards for bullet trajectory analysis, ricochet analysis typically requires outside input from firearm examiners. Additionally, current research regarding ricochet on wood surfaces remains less thoroughly investigated than ricochet analysis on other yielding surfaces. The goal of this research was to …

Transfer Of Material Derived From Polymer-Jacketed Bullets During Cycling And Discharge, Ethan A. Frazer

Student Theses

The goal of this study was to determine alternative ways to connect a polymer-coated bullet to the handgun from which it was fired. Polymer-coated bullets are lead projectiles encased in a synthetic coating, which may vary between brands and/or models, to reduce barrel friction and heavy metal exposure. Federal Syntech^® Total Synthetic Jacket (TSJ) ammunition presents unique challenges to firearms examiners as its synthetic coating may not retain the individualizing striations found in traditional unjacketed or metal-jacketed bullets.

In this study, 9mm TSJ ammunition was fired through multiple traditionally rifled aftermarket Glock barrels. Cloth targets were set up at …

Overlooking The Utility Of The Tattoo As An Investigatory Lead: An Exploratory Study Of Tattoo Documentation Quality Among Investigators And Medical Examiners, Deanna Corsetti

Student Theses

Tattoos have not only served as a subject of interest across cultures and disciplines for centuries, but also serve as a critical component of forensic investigations. Tattoos as traces–when properly identified and documented–serve as a valuable investigatory lead in cold, missing persons and unidentified remains cases. By examining the locations, dimensions, colors, and overall imagery of tattoos, investigators are able to use tattoos as a method of rapid, tentative identification of human remains (Miranda 2019). This study seeks to evaluate the quality and scope of tattoo documentation in cases classified as being unsolved. Specifically, tattoo data was collected from redacted …