Forensic Science and Technology Commons^™

Current Applications And Limitations Of Forensic Entomology, Helena Volckaert

Themis: Research Journal of Justice Studies and Forensic Science

Forensic entomology is the study of arthropods concerning their application in crime scene investigation as a method of predicting postmortem interval (PMI) in deceased or missing individuals. PMI is determined by the life stage of insects present on-scene. Insects used in criminal investigations are primarily beetles, flies, or moths; this may also include various scavenging insects and hitchhiking mites. These creatures help colonize decayed tissue, providing crime scene personnel with live specimens collected and examined for biological evidence. Forensic entomology is currently a niche application in most investigations—as qualified entomologists are often needed—and proper analysis of collected evidence can be …

Qualitative Analysis Of Luminol Efficacy On Bleach-Cleaned And Paint-Concealed Blood, Adam Wykoff

Themis: Research Journal of Justice Studies and Forensic Science

The presence of blood at a crime scene can provide investigators with a treasure trove of information regarding the nature and circumstances of a particular crime and aid in crime scene reconstruction; however, attempts at concealing blood are common scenarios. The development of chemiluminescent and fluorescent-based presumptive tests, such as the luminol and fluorescein tests, have made it more challenging to definitively remove or mask blood on a surface. The purpose of this experiment was to qualitatively measure the overall efficacy of luminol, concerning its ability to positively detect small bloodstains found on common household floor surfaces (wood, carpet, and …

Analysis Of Cannabinoids In Serum By Gc-Ms/Ms, Christie Cannarozzi

Student Theses

Due to recent changes in federal and state legislations, the availability and consumption of cannabis products have increased in the United States. The expanded use of recreational and medicinal cannabis products increases the importance of implementing sensitive and selective instrumental methods in toxicological laboratories, as legal implications may arise in forensic cases, such as driving under the influence of drugs (DUID). The purpose of this study was to perform a cross-validation for the quantitative analysis of cannabinoids (Δ9-tetrahydrocannabinol, cannabidiol, cannabinol, 11-hydroxy-Δ9-tetrahydrocannabinol, and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol) in serum by gas chromatography coupled to triple quadrupole tandem mass spectrometry (GCTQ). This method was fully …

Discrimination Of Monozygotic Twins Using Dna Methylation Levels Of One Cpg Site At Chromosome 3, Dino O. Robinson

Student Theses

Conventional STR typing, commonly used in forensics for human identification, poses a problem in criminal cases and paternity disputes involving monozygotic (MZ) twins because they share identical DNA sequences. To date, no routine method is available in forensics to differentiate between individuals of MZ pairs. Recently, epigenetic methods measuring differential DNA methylation patterns have been applied to MZ twin differentiation. In this study, we investigated the potential to identify MZ twins using a previously identified DNA methylation site in chromosome 3, cg18562578, in a sample of 129 MZ and 37 dizygotic (DZ) twin pairs. We used bisulfite converted saliva DNA …

Discrimination Of Soil Organic Matter Using Nuclear Magnetic Resonance In Conjunction With Principal Component Analysis, Victoria G. Hsieh

Student Theses

The characterization of trace evidence is key to the field of forensic science. Trace evidence can take many forms, such as glass, fiber, and soil. Traditionally, forensic soil analysis has been painted as one of the more tedious applications, which conventionally studies the mineral composition of soil samples. In this study, we present another avenue of soil analysis that takes advantage of understudied factor of soil: soil organic matter (SOM). Using both proton (¹H NMR) and carbon nuclear magnetic resonance (¹³C NMR) separately and applying principal component analysis (PCA) to both types of spectra, we sought …

The Ability Of Vitreous Metabolite Concentration To Determine Time Of Death, Victoria Wiggins

Themis: Research Journal of Justice Studies and Forensic Science

This paper analyzes the ability of vitreous fluid to determine time of death. The high variability in current methods used to establish post-mortem interval (PMI), or the amount of time between death and post-mortem examination make it difficult to obtain an accurate estimation. The concentration of metabolites found within vitreous fluid, such as potassium (K+), hypoxanthine (Hx), and magnesium (Mg), are the most researched alternatives to traditional methods in PMI determination because of the relative isolation of vitreous within the body and its ability to withstand degradation. There is no clear consensus on which metabolite provides the most accurate data …

Analyses Of Prosecutorial Power And Discretion In Mississippi: Evaluating Proposals To Address Misconduct And Abuse, Lucy Pruitt

Honors Theses

This thesis seeks to create a policy proposal in order to address incidences of prosecutorial misconduct and abuse of discretion in the Mississippi criminal justice system. To do so, the author has summarized and analyzed seven criminal cases in which defendants have become victims of prosecutorial misconduct in order to shed light on the lack of prosecutorial accountability in the state’s criminal justice system. In an attempt to solve the problem, the author has developed a novel grading rubric in order to objectively and systematically analyze and evaluate previously proposed policy recommendations by legal experts and justice organizations. The successes …

Digital Forensic Readiness: An Examination Of Law Enforcement Agencies In The State Of Maryland, James B. Mcnicholas Iii

Masters Theses & Doctoral Dissertations

Digital forensic readiness within the law enforcement community, especially at the local level, has gone mostly unexplored. As a result, a current lack of data exists that examines the digital forensic readiness of individual agencies, the possibility of proximity relationships, and correlations between readiness and backlogs. This quantitative, crosssectional research study sought to explore these issues by focusing on the state of Maryland. The study resulted in the creation of a digital forensic readiness scoring model that was then used to assign digital forensic readiness scores to thirty (30) of the one-hundred-forty-one (141) law enforcement agencies throughout Maryland. It was …

Program And Proceedings: Nebraska Academy Of Sciences 1880–2020, 140th Anniversary Year, One Hundred-Thirtieth Annual Meeting

Nebraska Academy of Sciences: Programs and Proceedings

No abstract provided.

Digital Forensics Tools Integration, Alexander D. Kim

Theses and Dissertations

As technology has become pervasive in our lives we record our daily activities both intentionally and unintentionally. Because of this, the amount of potential evidence found on digital media is staggering. Investigators have had to adapt and change their methods of conducting investigations to address the data volume. Digital forensics examiners current process consists of performing string searches to identify potential evidentiary items. Items of interest must then go through association, target comparison, and event reconstruction processes. These are manual and time consuming tasks for an examiner. This thesis presents a user interface that combines both the string searching capabilities …

The Effects Of The Evidence Preservation System (Eps) On The Storage Of Dna Samples, Devin Doyle

Graduate Student Research Symposium

Storage of collected samples is a concern for all disciplines of forensic science. Without proper storage, especially DNA samples, the DNA will become unusable as it could become degraded, cross contaminated with other samples, etc. Much of the preliminary research revealed that there were very few studies on the storage of forensic samples regarding ideal protocols, let alone those that involved combining as many controlled storage aspects such as temperature, humidity, and UV exposure. This research focuses on the Evidence Preservation System (EPS) by Forensic Solutions, Inc., which is a controlled environment able to be programmed to control temperature and …

Review Of Fundamental To Know About The Future, Hannarae Lee

International Journal of Cybersecurity Intelligence & Cybercrime

What we consider fundamental elements can be easily overlooked or perceived as facts without the process of empirical testing. Especially in the field of cybercrime and cybersecurity, there are more speculations regarding the prevalence and the scope of harm carried out by wrongdoers than empirically tested studies. To fill the void, three articles included in the current issue addresses empirical findings of fundamental concerns and knowledge in the field of cybercrime and cybersecurity.

Illegal Gambling And Its Operation Via The Darknet And Bitcoin: An Application Of Routine Activity Theory, Sinyong Choi, Kyung-Shick Choi, Yesim Sungu-Eryilmaz, Hee-Kyung Park

International Journal of Cybersecurity Intelligence & Cybercrime

The Darknet and Bitcoins have been widely utilized by those who wish to anonymously perform illegal activities in cyberspace. Restricted in many countries, gambling websites utilize Bitcoin payments that allow users to freely engage in illegal gambling activities with the absence of a formal capable guardian. Despite the urgency and limited knowledge available to law enforcement regarding this issue, few empirical studies have focused on illegal gambling websites. The current study attempts to examine the characteristics and operations of online gambling websites on both the Darknet and Surface Web, which allow Bitcoin payments. The findings suggest that both websites on …

An Empirical Study To Determine The Role Of File-System In Modification Of Hash Value, Kumarshankar Raychaudhuri, M. George Christopher

International Journal of Cybersecurity Intelligence & Cybercrime

In digital forensics, maintaining the integrity of digital exhibits is an essential aspect of the entire investigation and examination process, which is established using the technique of hashing. Lack of knowledge, while handling digital exhibits, might lead to unintentional alteration of computed hash, rendering the exhibit unacceptable in the court of Law. The hash value of a physical drive does not solely depend upon the data files present in it but also its file-system. Therefore, any change to the file-system might result in the change of the disk hash, even when the data files within it remain untouched. In this …

A Reverse Digital Divide: Comparing Information Security Behaviors Of Generation Y And Generation Z Adults, Scott M. Debb, Daniel R. Schaffer, Darlene G. Colson

International Journal of Cybersecurity Intelligence & Cybercrime

How individuals conceptualize their accountability related to digital technology. There may also be age-based vulnerabilities resulting from personal perceptions about the importance of engaging in best-practices. However, age may not be as critical as experience when it comes to implementation of these behaviors. Using the Cybersecurity Behaviors subscale of the Online Security Behaviors and Beliefs Questionnaire (OSBBQ), this study compared the self-reported cybersecurity attitudes and behaviors across college-aged individuals from Generation Y and Generation Z. Data were derived from a convenience sample of predominantly African-American and Caucasian respondents (N=593) recruited from two public universities in Virginia, USA. Four of the …

Book Review: The Cyber Risk Handbook By Domenic Antonucci, Stanley Mierzwa

International Journal of Cybersecurity Intelligence & Cybercrime

No abstract provided.

Investigating The Potential And Pitfalls Of Ev-Encapsulated Micrornas As Circulating Biomarkers Of Breast Cancer, Brian M. Moloney, Katie E. Gilligan, Doireann P. Joyce, Clodagh P. O'Neill, Killian P. O'Brien, Sonja Khan, Claire L. Glynn, Ronan M. Waldron, Ciarán M. Maguire, Emma Holian, Erin Naughton, Mohamed Elhadi, Andrea B. Grealish, Carmel Malone, Emma Mcdermott, Peter Dockery, Thomas Ritter, Adriele Prina-Mello, Michael J. Kerin, Róisín M. Dwyer

Forensic Science Publications

Extracellular vesicles (EVs) shuttle microRNA (miRNA) throughout the circulation and are believed to represent a fingerprint of the releasing cell. We isolated and characterized serum EVs of breast tumour-bearing animals, breast cancer (BC) patients, and healthy controls. EVs were characterized using transmission electron microscopy (TEM), protein quantification, western blotting, and nanoparticle tracking analysis (NTA). Absolute quantitative (AQ)-PCR was employed to analyse EV-miR-451a expression. Isolated EVs had the appropriate morphology and size. Patient sera contained significantly more EVs than did healthy controls. In tumour-bearing animals, a correlation between serum EV number and tumour burden was observed. There was no significant relationship …

Potential Applications Of Microrna Profiling To Forensic Investigations, Claire L. Glynn

Forensic Science Publications

Within the forensic science community, there is a continued push to develop novel tools to aid in criminal investigations. microRNA (miRNA) analysis has been the focus of many researcher’s attention in the biomedical field since its discovery in 1993; however, the forensic application of miRNA analysis has only been suggested within the last 10 years and has been gaining considerable traction recently. The primary focus of the forensic application of miRNA analysis has been on body fluid identification to provide confirmatory universal analysis of unknown biological stains obtained from crime scenes or evidence items. There are, however, other forensic applications …

The Costs And Benefits Of Forensics, Brandon L. Garrett

Faculty Scholarship

Supreme Court Justice Louis Brandeis famously wrote that states can be laboratories for experimentation in law and policy. Disappointingly, however, the actual laboratories that states and local governments run are not a home for experimentation. We do not have adequate information about either the costs or the benefits of forensic testing or allocation of resources. Increased spending and expansion of crime laboratories has perversely accompanied growing backlogs. Poor quality control has resulted in a series of audits and even closures of crime laboratories. In response to these problems, however, some laboratories and some entire states have developed new approaches toward …

Justice In Forensic Algorithms, Brandon L. Garrett

Faculty Scholarship

No abstract provided.

Postmortem Interval Estimation Based On Eukaryotic Community Associated With Soil Under Decomposing Porcine Remains, Amanda M. Haase

Master of Science in Forensic Science Directed Research Projects

Recent studies have shown that bacteria associated with soils under a carcass change significantly both temporally and spatially. These studies were done in both laboratory and field conditions, but with limited replications. In addition, limited information exists on temporal and spatial changes in eukaryotic community structure associated with soil under decomposing remains. This study was designed to fill in these gaps and expand on previous studies to improve postmortem interval (PMI) estimation techniques as well as to identify cadaver decomposition islands (CDI) based on eukaryotic community structure associated with soil under vertebrate remains. To accomplish this goal, soil samples were …

From The Legal Literature: Automating Police, Francesca Laguardia

Department of Justice Studies Faculty Scholarship and Creative Works

No abstract provided.

Evaluating The Use Of The M-Vac® Wet Vacuum System To Recover Dna From Cotton Fabric, Phillip Reilly Irion

Graduate Theses, Dissertations, and Problem Reports (ETD)

With the current state of the field of forensic biology, collecting DNA from evidence samples may pose some difficulty. Traditional methods for collection of DNA from biological fluids and touch samples include cuttings, scrapings, and the double swab method. While all of these methods have benefits, they also have their drawbacks. In order to more efficiently collect DNA from an evidence sample, novel approaches need to be explored. This novel approach is the microbial vacuum (M-Vac®) Wet Vacuum System.

This system utilizes a sterile collection solution and a vacuum to collect DNA material from evidence samples. While it is similar …

Statistical Assessment Of The Significance Of Fracture Fits In Trace Evidence, Evie K. Brooks

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fracture fits are often regarded as the highest degree of association of trace materials due to the common belief that inherently random fracturing events produce individualizing patterns. Often referred to as physical matches, fracture matches, or physical fits, these assessments consist of the realignment of two or more items with distinctive features and edge morphologies to demonstrate they were once part of the same object. Separated materials may provide a valuable link between items, individuals, or locations in forensic casework in a variety of criminal situations. Physical fit examinations require the use of the examiner’s judgment, which rarely can be …

Detection Of Bacterial Lipopolysaccharides Of Escherichia Coli By Direct Analysis In Real Time/Time-Of-Flight Mass Spectrometry, Dakotah M. Biller

Master of Science in Forensic Science Directed Research Projects

Lipopolysaccharides (LPS) are a major component of the outer membrane in Gram-negative bacteria species and are responsible for toxicity caused by organisms such as Escherichia coli (E.coli). Conventional methods for characterizing LPS utilize mass spectrometry, particularly matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) and liquid chromatography mass spectrometry (LC-MS). Although these are successful in analyzing intact LPS molecules, they can be time-consuming. Other mass spectrometry techniques, such as direct analysis in real time/time-of-flight mass spectrometry (DART-TOF-MS) could also be helpful in the rapid analysis of LPS molecules without sample extraction procedures. This research focuses on the application …

The Utilization Of Sex Hormone Antibodies For Screening And Separation Of Trace Biological Mixtures, Kristin N. Jones

Master of Science in Forensic Science Directed Research Projects

Touch or trace evidence consists of epidermal cells deposited by contact with items such as handled objects, touched surfaces, or worn clothes. This type of evidence has surpassed most other sample types submitted to forensic labs and typically consists of low quantities of DNA and multiple contributors. In this study epithelial skin cells, i.e., “touch/trace evidence,” were used as they are estimated to constitute approximately half of the casework evidence items submitted for DNA analysis. For the optimization of antibody staining, male and female skin epithelial samples from donors were incubated and hybridized with antibodies of various concentrations of Alexa …

Development Of An Untargeted Gas Chromatography-Mass Spectrometry (Gc/Ms) Method For The Detection Of Drugs In Wastewater, Samuel A. Miller

Master of Science in Forensic Science Directed Research Projects

Monitoring current illicit drug trends and consumption rates of pharmaceuticals using a non-invasive collection technique is needed to address the present drug use and the growing drug epidemic. Reliance on self-reporting drug use surveys is not always a practical measure of illicit drug use. Wastewater analysis has been used globally as a targeted method for monitoring the consumption of specific illicit drugs. Current existing analytical techniques for wastewater analysis focus on the use of targeted liquid chromatography-mass spectrometry (LC-MS) based techniques. Few gas chromatography (GC) procedures exist for wastewater analysis, and those that do concentrate their methods on a single …

Development Of A Quantitative Pcr (Qpcr) Based Method For Studying Temporal Dna Degradation In Waterlogged Bone, Isis Thornton

Master of Science in Forensic Science Directed Research Projects

Human activities are often centered around the presence of water, thus it is not surprising that there are many water-related human deaths. Accumulated degree days (ADD), and other aquatic variables may affect DNA retrieval from waterlogged bone. Calcium and collagen in bone can inhibit the PCR necessary to produce an STR profile; the current solution is a time-consuming organic extraction. While there are examples of research on DNA degradation in terrestrial bone over time, there has been little work done on submerged bone samples and they are usually limited to case studies. The major aim of this study was to …

Expanded Testing Of An Integrated Qpcr Mixture Screening Assay Using High Resolution Melt Curve Analysis And Support Vector Machine Modeling, Andrea L. Williams

Master of Science in Forensic Science Directed Research Projects

In the conventional forensic DNA workflow, the number of contributors in a sample is unknown until the final step of STR analysis. We propose a high-resolution melt curve (HRM) mixture screening assay, which uses support vector machine (SVM) modeling of melt morphologies of D5S818 and D18S51 amplicons integrated into a common qPCR-based human DNA quantification kit, to differentiate between single-source samples (and their genotypes) and mixtures at an earlier stage in the DNA workflow (quantification). Previously, using data generated from whole melt curves, 87.5% of single-source samples and 100% of 1:1 mixture samples (2 contributors) classified accurately.

In this study, …

The Effect Of Time And Temperature On The Quality Of Latent Fingerprints On Incandescent Lightbulbs, Varying Donors Age And Sex, Kinaysha M. Collazo Maldonado

Master of Science in Forensic Science Directed Research Projects

Fingerprints are used as a means of identification, but there are no established methodologies to determine time since deposition of latent fingerprints by visual means alone. This research considered the influence of age and sex on the quality of recovered latent prints from lit and unlit lightbulbs from 1 to 10 days, using accumulated degree hours (ADH) to account for both heat and time simultaneously. Two male and two female donors (one of each aged40 years) were used. A thermal imaging camera was used to monitor the lightbulbs top and middle regions, which were significantly different (p≤0.05) for the experimental …