Forensic Science and Technology Commons^™

Interacting With Ideas: How To Engage Stem Students In Active Learning Of Theory Using Technology, Jessica Elizabeth Whitney, Keith Brian Morris

2026 Scholarly Teaching Conference: Concurrent Session Papers

STEM education in the modern age has been subject to much reform – from the integration of technology to an emphasis on student-centered teaching strategies, such as active learning. However, in the wake of virtual and blended-learning environments, student engagement and teacher assessment of student success have been challenged. Tools such as KAHOOT! and iClicker have been promoted to foster an active learning environment while sometimes falling short in regards to student retention of course material. In light of this technological educational revolution, instructors need to be able to determine the most effective tools for their discipline to aid in …

Assessment Of Analytical Workflows Of Gunshot Residue Evidence And Strategies For Implementation Of Advanced Technology In Crime Laboratories., Leah D. Thomas

Graduate Theses, Dissertations, and Problem Reports (ETD)

The rise in gun violence in the United States has increased the demand on forensic agencies to process evidence promptly and accurately. The exchange of imperceptible traces among objects, suspects, and victims can provide critical insights into the interactions and sequence of events during a crime. Gunshot residue (GSR) is a type of trace evidence that holds significant investigative value in shooting reconstructions. GSR can help identify potential shooters, intermediate targets, and bullet trajectories; however, its analysis is often expensive and time-consuming. Consequently, there is an urgent need for more effective solutions.

Methods for on-site screening can significantly streamline forensic …

Analysis Of Shotshell Breech Face Impressions Using A Computer-Based Algorithm, Carly Renee Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forensic firearm analysis involves the comparison of test fires from a suspect firearm to an unknown sample, typically collected from a crime scene. Test fires and the unknown sample are examined for agreement in class characteristics and sufficient agreement in individual characteristics using a comparison microscope. The forensic analysis of firearms has been challenged both in court and by governmental organizations for its subjective nature and the inability to accurately report error rates.

To combat these challenges, the National Institute of Standards and Technology (NIST) has proposed using computer-based algorithms, such as Congruent Matching Cells (CMC). These CMC algorithm calculates …

Repeatability And Reproducibility Of Minutiae Mark-Up, Lyrek Lockley

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study investigated the repeatability and reproducibility of minutiae mark-up. The use of fingerprints as a mode of identification has been around for thousands of years with contributions from figures like Sir William Hershel, Thomas Taylor, Henry Faulds, Sir Francis Galton and many more. Latent fingerprint impressions can be deposited by the perpetrator of a crime on a variety of surfaces at a crime scene. Many times, fingerprints are the most widely recovered and used evidence from a crime scene. Once fingerprints are collected, they go through the analysis, comparison, evaluation, and verification (ACE-V) process. This study will focus on …

Footwear Image Quality Classification: Using Subjective Assessments And Objective Image Metrics To Predict Impression Quality, En-Tni Lin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Shoeprints deposited during the commission of a crime vary in quality as a function of numerous factors, such as substrates, media, and the specific physical activities carried out by perpetrators. This variability in impression quality impacts the amount of information available from questioned crime scene impressions, and therefore an examiner's confidence in providing opinions of source attribution. In response, this research attempted to characterize footwear impression quality based on image sharpness, contrast, entropy, homogeneity, noise, quantity of information, and complexity, and to integrate these hand-engineered feature descriptions with subjective opinions of impression quality.

The methodology followed a five-pronged approach. First, …

Evaluating The Degradation Relationship Between Latent Print Impressions And Touch Dna Under Varying Environmental Conditions, Katrina M. Rupert B.S.

Graduate Theses, Dissertations, and Problem Reports (ETD)

Latent prints have been processed as a source of touch DNA evidence in the hopes of attaining partial or even full DNA profiles. However, the relationship between the latent quality and the DNA quality has not yet been fully explored, resulting in gaps in understanding how the two are connected. A common source of touch DNA is from latent print evidence, which is typically processed and photographed at a scene, and subsequently processed for DNA in the laboratory. However, there is skepticism in the forensic community around touch DNA as laboratory procedures are often manipulated beyond standard operating procedures to …

Strengthening The Interpretation Of Glass And Paint Evidence Through The Study Of Random Frequency Of Occurrence And Analytical Information, Lauryn C. Alexander

Graduate Theses, Dissertations, and Problem Reports (ETD)

Glass and paint particles are frequently discovered at crime scenes as valuable trace evidence. Often, these fragments are transferred from a crime scene to a victim or person-of-interest, and can subsequently be evaluated, analyzed, and classified according to their physical and optical measurements, and chemical properties, each of which can provide important information to the analyst to assist in the determining if a recovered particle is distinguishable or indistinguishable from a known source. Moreover, these minute materials can provide information about how the events took place, leading to the reconstruction of crime scenes and the identification of valuable clues for …

Feature Extraction Of Footwear Impression Images For Quality Assessment, Alexandra Hill

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forensic footwear impression analysis is a valuable tool in criminal investigations. Extracting useful features from images of footwear impressions is a critical step in this process. However, the quality of these images can vary widely, making feature extraction challenging. In order to give a quality assessment rating to a footwear impression image, the image should first be analyzed to extract features from the impression. In this paper, we present a method to extract features from a 2D grayscale footwear impression image. A Hierarchical Grid Model implementation has been adapted from use on a 3D dataset to assist in finding features, …

Development Of A Mass Transfer Correction Factor In A Thermodynamic Model To Explain The Weathering Patterns Of Ignitable Liquids On Household Substrates At Elevated Temperatures, Max T. Denn

Graduate Theses, Dissertations, and Problem Reports (ETD)

To determine whether a questioned fire is the result of arson or not, forensic chemists will often attempt to extract and identify any ignitable liquid residues present in the fire debris. Forensic chemists use gas chromatography-mass spectrometry (GC-MS) to identify ignitable liquids based on similarities between chromatograms of fire debris extracts and those of reference ignitable liquids that have been evaporated (weathered) to different extents. Casework samples often appear to be relatively unweathered (e.g., 40-60%) and conventional wisdom is that the apparent lack of weathering is the result of entrapment; a process by which liquid residues are absorbed into the …

Evaluation Of Emerging Screening Technologies For The On-Site Detection And Identification Of Methamphetamine And Its Precursors In Simulated Clandestine Lab Operations, Alexis Nicole Wilcox

Graduate Theses, Dissertations, and Problem Reports (ETD)

Stimulant drugs comprise one of the top drug categories abused in the United States. Due to its accessibility, low price, and manufacturing simplicity, methamphetamine is frequently placed within the top 10 seized drugs in the country. As of March 2023, methamphetamine is the most seized controlled substance in the United States, with 34,291 kg. In 2022, the United States seized over 79,000 kg of methamphetamine. One reason for the proliferation of methamphetamine is related to the production itself, which does not require large warehouses but can be manufactured in houses using relatively accessible materials and small containers. When a clandestine …

Assessment Of Acquisition, Retention, And Evolution Of Randomly Acquired Characteristics With Wear, Nathaniel Weston

Graduate Theses, Dissertations, and Problem Reports (ETD)

The recovery of known-source shoes for the purpose of comparison to crime scene impressions often occurs with a temporal lag. During this passage of time, the outsole can be altered due to continued wear. These changes may impact forensically relevant characteristics of use known as randomly acquired characteristics (RACs). Continued wear may cause the formation of new RACs, cause RACs to undergo some degree of geometric change, and/or lead to the loss of formerly existing RACs. Consequently, the correspondence between a test impression from a known mated shoe with continued wear and a questioned impression with less wear deposited at …

An Assessment Of The Forensic Aspects Of Genetic Genealogy, Sarah Hester

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forensic genetic genealogy has grown in both popularity and controversy in recent years. The genetic information comes from large numbers of single nucleotide polymorphism (SNP) markers. Polymorphism variations can be traced through familial lineage, determining how closely individuals are related to each other based on the similarities and differences in SNPs within their DNA. Polymorphisms account for approximately 85% of human variation. Genealogy and ancestry determination companies trace the lineage of a person back through multiple generations using polymorphisms. As more individuals submit their DNA for analysis the genealogical profile databases continue to grow in size. Law enforcement uses genealogical …

Analysis Of Forensically Relevant Evidence Using Electrochemistry, Spectroscopy, And Mass Spectrometry Tools, Colby Edward Ott

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forensic science relies on the use of multiple techniques in the assessment of evidence to increase the accuracy and reliability of the results. However, with the rapidly changing drug landscape due to the introduction of novel psychoactive substances, many traditional screening methods are no longer sensitive or selective enough for use. Additionally, many screening methods such as chemical color tests are prone to false positive and negative results and are subjective. Therefore, the goal of this dissertation was to develop a novel analytical scheme that can provide a more efficient, rapid, and sensitive method that will facilitate adoption in the …

Assessment Of Quechers Extraction Protocol For The Lc-Ms/Ms Detection Of Emerging Fentanyl Analogs In Biological Specimens For Clinical And Forensic Purposes, Kylea M. Morris

Graduate Theses, Dissertations, and Problem Reports (ETD)

Drug abuse within the United States is a dynamic and pervasive issue that affects the quality and life expectancy of our society. One of the major drug classes that are constantly utilized is opioids. Opioids are prescribed for pain management in patients suffering from cancer and other medical illnesses. Long-term use of opioids could lead to tolerance in humans, which poses a risk of reaching fatal overdose levels. Unfortunately, opioids are abused recreationally, and in some instances, individuals are unaware of the presence of dangerous synthetic drugs like fentanyl, hidden in materials such as heroin, cocaine, oxycodone, and methamphetamine. Recent …

Development Of An Expert Algorithm For Substance Identification (Easi) To Discriminate Between Spectrally Similar Fentanyl Analogs In Mass Spectrometry, Alexandra Adeoye

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fentanyl is an opioid that is about 100 times more potent than morphine, and its analogs are just as potent, and some like carfentanil are even more so potent. In recent years, fentanyl and its analogs have been responsible for over 50% of overdose-related deaths. In response, the DEA has placed all fentanyl analogs as Schedule I compounds because of their non-medicinal use and high potential for abuse and physical dependence. However, fentanyl analogs have high structural and mass spectral similarities due to the extremely conservative nature of the core fentanyl structure, which makes the differentiation and identification of fentanyl …

Analyzing The Viability Of Direct Pcr For Use In Conjunction With Cyanoacrylate Enhanced Fingerprints, Coral M. Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Efficient methods for DNA analysis are desperately needed in laboratories due to the influx in DNA analysis requests. The current DNA processing methods are costly, time consuming, and involve multiple tube changes, increasing the risk of contamination. However, the use of direct PCR can simplify the DNA analysis process by eliminating the extraction, purification and quantitation steps. Presently, the FBI Quality Assurance Standard 9.4 requires all evidence samples to be quantitated. As a result, the direct PCR method of DNA processing cannot be implemented on evidence samples at this time. Direct PCR presents the opportunity to provide efficient DNA analysis, …

Mtdna Heteroplasmy In Hair Shafts Versus Buccal Swabs For Forensic Applications, Sara R. Bodnar

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mitochondrial DNA (mtDNA) analysis has several forensic applications such as criminal investigations, identification of human remains, and missing person investigations. It is also the only type of DNA that is available from certain sample types such as hair shafts. The presence of mtDNA heteroplasmy within and between tissue types can lead to mtDNA sequence differences when comparing samples originating from the same individual. Studies on mtDNA heteroplasmy are increasingly being carried out for their implications in forensic interpretation of mtDNA sequences. Specifically, mtDNA in buccal swabs compared to hair samples from one individual may show differences in sequence due to …

Evaluating The Use Of The M-Vac® Wet Vacuum System To Recover Dna From Cotton Fabric, Phillip Reilly Irion

Graduate Theses, Dissertations, and Problem Reports (ETD)

With the current state of the field of forensic biology, collecting DNA from evidence samples may pose some difficulty. Traditional methods for collection of DNA from biological fluids and touch samples include cuttings, scrapings, and the double swab method. While all of these methods have benefits, they also have their drawbacks. In order to more efficiently collect DNA from an evidence sample, novel approaches need to be explored. This novel approach is the microbial vacuum (M-Vac®) Wet Vacuum System.

This system utilizes a sterile collection solution and a vacuum to collect DNA material from evidence samples. While it is similar …

Statistical Assessment Of The Significance Of Fracture Fits In Trace Evidence, Evie K. Brooks

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fracture fits are often regarded as the highest degree of association of trace materials due to the common belief that inherently random fracturing events produce individualizing patterns. Often referred to as physical matches, fracture matches, or physical fits, these assessments consist of the realignment of two or more items with distinctive features and edge morphologies to demonstrate they were once part of the same object. Separated materials may provide a valuable link between items, individuals, or locations in forensic casework in a variety of criminal situations. Physical fit examinations require the use of the examiner’s judgment, which rarely can be …

Statistical Evaluation Of Randomly Acquired Characteristics On Outsoles With Implications Regarding Chance Co-Occurrence And Spatial Randomness, Nicole Richetelli

Graduate Theses, Dissertations, and Problem Reports (ETD)

Footwear evidence holds tremendous forensic value, owing to its ability to formulate linkages between victims, suspects and scenes. Naturally, the strength of these linkages is a function of the perceived clarity, quality and rarity of class, subclass and randomly acquired characteristics (RACs), which are the fundamental outsole features used to formulate source associations. In order to reach a conclusion when performing a footwear comparison, forensic examiners must assign value to the observed similarities and differences that exist between questioned crime scene and test impressions. Embedded within this process is an evaluation of the random association between unrelated shoes as a …

Spatio-Temporal Analysis Of Crime Incidents For Forensic Investigation, Jamie Spencer Spaulding

Graduate Theses, Dissertations, and Problem Reports (ETD)

Crime analysis and mapping has been routinely employed to gather intelligence which informs security efforts and forensic investigations. Traditionally, geographic information systems in the form of third-party mapping applications are used for analysis of crime data but are often expensive and lack flexibility, transparency, or efficiency in uncovering associations and relationships in crime. Each crime incident and article of evidence within that incident has an associated spatial and temporal component which may yield significant and relevant information to the case. Wide variations exist in the techniques that departments use and commonly spatial and temporal components of crime are evaluated independently, …

Validation Of Fast Spectrochemical Screening Methods For The Identification Of Counterfeit Pharmaceutical Packaging, Emily Ann Haase

Graduate Theses, Dissertations, and Problem Reports (ETD)

Counterfeit pharmaceuticals are an actively developing health and economic threat worldwide. Particularly prevalent are counterfeit pharmaceuticals distributed in emerging nations and through internet pharmacies or e-pharmacies. Although technology has been developed that discourages anti-counterfeiting practices (such as optically variable devices, invisible ink, and track-and-trace technology), it remains somewhat novel and expensive to implement on a widespread scale.

In this study, Laser Induced Breakdown Spectroscopy (LIBS) and Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) were proposed as fast and non-invasive tools for the identification of counterfeit pharmaceutical packages. The main objective of this research was to develop and evaluate the capabilities …

The Effects Of Household Substrates On The Evaporation Of Ignitable Liquids At Temperatures Up To 210℃, Caitlyn Wensel

Graduate Theses, Dissertations, and Problem Reports (ETD)

In fire debris analysis, ignitable liquid residues are commonly identified using gas chromatography-mass spectrometry (GC-MS). The detection and identification of an ignitable liquid can help arson investigators determine whether a fire was intentional or accidental. To assist with the identification of ignitable liquid residues, chromatograms of questioned samples are typically compared to those of known ignitable liquids that have been weathered (evaporated) to different extents. Practitioners typically perform such weathering at room temperature and to a limited number of extents of weathering, so their database of weathered residues is likely to deviate markedly from casework residues. The ultimate goal of …

Screening Sexual Assault Evidence With Low Concentrations Of Male Dna Utilizing The Rapidhit 200 And Paradna Intelligence Test, Taylor L. Koepfler

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over the last several years, crime laboratories have largely focused on the sexual assault kit (SAK) backlog, where they are often confronted with many low-quality samples. The lack of available screening techniques has prevented analysts from gaining insight into the disposition of the sample earlier on in the testing process; requiring analysts to rely on visual observations and little background information. Consequently, resulting in the hindrance of probative STR profiles while expending a large amount of time and resources to gain this result. In recent years, crime laboratories have explored a male screening technique, recommended by SWGDAM, to combat this …

Assessing The Reliability Of Physical End Matching And Chemical Comparison Of Pressure Sensitive Tapes, Meghan Nicole Prusinowski

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pressure sensitive tapes are a common evidence type in a variety of forensic cases, including violent crimes, drug trafficking, and terrorism. Forensic laboratories are often requested to analyze tape samples for composition and potential source identification. Tape samples manufactured from different sources are often distinguishable when using a combination of sensitive analytical techniques. Nonetheless, the interpretation of the data remains challenging due to a lack of standard criteria and guidelines from which examiners can support their conclusions. As a result, there is a need to standardize the protocols of interpretation of physical and chemical comparisons of tape evidence.

The manufacture …

Compound-Specific Isotope Analysis Of Amino Acids In Biological Tissues: Applications In Forensic Entomology, Food Authentication And Soft-Biometrics In Humans, Mayara Patricia Viana De Matos

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this work we demonstrate the power of compound-specific isotope analysis (CSIA) to analyze proteinaceous biological materials in three distinct forensic applications, including: 1) linking necrophagous blow flies in different life stages to their primary carrion diet; 2) identifying the harvesting area of oysters for food authentication purposes; and 3) the ability to predict biometric traits about humans from their hair.

In the first application, we measured the amino-acid-level fractionation that occurs at each major life stage of Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) blow flies. Adult blow flies oviposited on raw pork muscle, beef muscle, or chicken liver. Larvae, pupae …

Enhancing The Senses: How Technological Advances Shape Our View Of The Law, Steven Goldberg

West Virginia Law Review

No abstract provided.