Legal Studies Commons^™

Identification Of The New Synthetic Opioid Bucinnazine And Its Metabolites Using Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Karissa N. Resnik

Master of Science in Forensic Science Directed Research Projects

In the past 20 years, the rate of opioid use increased drastically in the United States. The country has experienced an enormous number of deaths caused by opioid overdose, opioid use disorder (OUD), as well as other harms as a consequence of the high numbers of opioid prescriptions. Fentanyl, a well-known synthetic opioid, has been a contributor to the opioid crisis since 2013 due to its popularity in clinical and illicit use. It has a high potency, which makes it an effective analgesic but a dangerous illicit substance. A new synthetic opioid, bucinnazine, has recently become a new black-market opioid …

The Analysis Of Commercially Available Kratom Products In Richmond, Virginia, James H. Fleming Jr

Master of Science in Forensic Science Directed Research Projects

Kratom is a novel psychoactive substance that has gained popularity within the past ten years. Originating from Southeast Asia, the leaves of the Mitragyna speciosa tree contain two principal alkaloids, mitragynine and 7-hydroxymitragynine, that play a key role in opioid-like effects. Twenty-nine kratom products were obtained from tobacco shops in the Richmond, Virginia area, including powders, teas, capsules, extracts, and a carbonated beverage. Samples were analyzed using Direct Analysis in Real Time-Mass Spectrometry (DART-MS) for kratom alkaloids, labeled ingredients, and other possible organic compounds. Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) was used to quantitate aluminum, arsenic, copper, iron, magnesium, nickel, …

Using A Non-Destructive Method To Estimate The Number Of Contributors And Predict Dna Yield In Touch Dna Mixtures, Christin Lee

Master of Science in Forensic Science Directed Research Projects

Touch DNA samples are low templates and often are mixtures, making them typically one of the most difficult types of evidence to interpret. Short tandem repeat analysis is a standard method to estimate the number of contributors and DNA yield for each contributor; however, there are some limitations. Applying a non-destructive pre-DNA analysis technique that provides information about the touch DNA may improve the casework process. Antibody probes to hormones such as testosterone and dihydrotestosterone (DHT), and proteins including cytokeratins (CK) inside epidermal cells, have been demonstrated to produce a personalized signature for human touch cell populations. Testosterone, DHT, and …

A Comparison Of Cyanoacrylate Fuming Followed By Mbd Dye Staining And Gun Bluing For Development Of Latent Fingerprints On Cartridge Cases, Jamie Zaleta

Master of Science in Forensic Science Directed Research Projects

The development of latent fingerprints on cartridge cases is a desirable ability for the forensic laboratory, offering the potential to provide probative evidence as to the handler of whole ammunition or fired cartridge cases discovered at the crime scene. Previous studies have focused primarily on intentionally-laid fingerprints on cartridge case surfaces, which do not represent samples likely to come across the desk of a forensic analyst. This study focuses on naturally-handled ammunition and the success rates associated with latent fingerprints produced in a more realistic set of circumstances. A comparison was performed of two fingerprint development methods—gun bluing and MBD …

Development Of A Loop-Mediated Isothermal Amplification (Lamp) Assay For Detection Of Mirnas, Alisha B. Whelan

Master of Science in Forensic Science Directed Research Projects

Body fluid identification serves a vital role in contextualizing biological evidence received in forensic laboratories. While several fluids can be presumptively detected using immunological or catalytic tests, these tests are susceptible to false results and have limited sensitivity. True confirmatory testing is still somewhat limited in forensic laboratories. With the advancement of DNA typing technologies, laboratories can develop DNA profiles from trace amounts of sample, increasing the demand for sensitive and accurate body fluid detection. This project proposes using loop-mediated isothermal amplification (LAMP) as a method to rapidly detect microRNAs (miRNAs) that are differentially expressed in various body fluids with …

Optimization And Comparison Of Methods For Separation Of Spermatozoa From Superabsorbent Polymer-Containing Forensic Evidence, Hannah Wells

Master of Science in Forensic Science Directed Research Projects

Sexual assault is currently one of the most prevalent crimes that affects victims of all ages. Forensic DNA analysts often confirm proof of contact in sexual assault cases through the identification of spermatozoa and subsequent STR profiling. Diapers and other feminine hygiene products, such as maxi pads and ultrathin pads, are types of superabsorbent polymer-containing evidence that complicate the process of DNA analysis due to the trapping of the spermatozoa in the gel-like matrix. In this study, a comparison of methods was performed to determine which extraction technique produces the highest sperm cell and DNA yield and generates a usable …

Stability And Variation Within Donors Of Mirna Markers For Body Fluid Identification, Kelsey R. Price

Master of Science in Forensic Science Directed Research Projects

Traditional enzymatic tests for body fluid identification are prone to false negatives, false positives, and several body fluids do not have confirmatory or reliable presumptive tests. Therefore, molecular-based tests may be more reliable, such as messenger RNA (mRNA), microbial DNA, or microRNA (miRNA) assays.

miRNAs are small, noncoding RNAs whose main function in-vivo is the regulation of protein expression by selectively suppressing the translation of their corresponding mRNAs. They also lack a poly-A tail, and because of this, their small size, and their association with other molecules such as the RISC protein complex, they are more robust than other RNAs, …

Optimization Of A Microfluidic Device For Cell Capture Using Optical Trapping, Samantha C. Pagel

Master of Science in Forensic Science Directed Research Projects

Biological mixtures which result from evidence produced in sexual assault cases are one of the most common types of mixtures observed in forensic biology casework and have contributed to evidence backlogs across the country. In the forensic community, this has highlighted the need for a more efficient cell separation method at the beginning of the DNA analysis workflow than the currently accepted method. One promising novel front-end cell separation technique is optical trapping. In heterogeneous mixtures, optical tweezers have been demonstrated to manipulate and isolate cells of interest from other cell types.

For this research, a range of 5 to …

Impact Of 1,4-Dithiothreitol (Dtt) On Microbial Community Structures Associated With Human Semen Samples, Christian R. Blaise

Master of Science in Forensic Science Directed Research Projects

Body fluid identification (BFID) is one of the most important steps in forensic science as it provides important context to how DNA analysis should be performed on biological samples found at the scene of a crime. Many scientific methods on BFID have been developed over the years with respective benefits and flaws. One emerging method in recent years is microbial signature based BFID. This method uses the same DNA extract that is utilized for human DNA profile generation for microbial analysis. Although DNA is extracted from all body fluid samples without any additional steps, extraction of human DNA from semen …

A Quantifiler Trio-Based Hrm Mixture Screening Assay For The Quantstudio 6 Flex Qpcr Platform, Dayanara A. Torres

Master of Science in Forensic Science Directed Research Projects

At present, the forensic DNA workflow is not capable of providing information about the contributor status (single source vs. mixtures) of evidentiary samples prior to end-point analysis. This shortcoming can exacerbate the challenges inherent to mixtures and low-template DNA samples. Provided additional sample information earlier in the workflow, protocols could be implemented to mitigate these challenges. High-resolution melt (HRM) curve analysis is a technique used to detect genetic variation in DNA fragments and in the last decade has been evaluated for use in differentiation of samples by genotype and/or contributor status. To this end, a proof-of-concept HRM assay using the …

Classification And Differentiation Of Forensically Relevant Cell Populations Using Imaging Flow Cytometry, Arianna D. Decorte

Master of Science in Forensic Science Directed Research Projects

Forensic casework relies heavily on DNA profiles which may be time-consuming to generate and difficult to interpret when biological mixtures are present. Additionally, there are significant challenges in using sub-source data alone to answer the activity-level questions often most pertinent in criminal cases. Source level information can add critical probative value, however, methods that can provide information as to the source tissue of evidentiary cell populations are limited. There remains a need for new methods that can differentiate between and classify various cell populations, particularly for vaginal and epidermal tissue, since there are no conventional serological techniques specific to these …

Determination Of Cotinine Concentration In Urine By Liquid Chromatography Tandem Mass Spectrometry, Allison Beckett

Master of Science in Forensic Science Directed Research Projects

Tobacco use can cause lasting health conditions which can lead to death. Tobacco exposure comes from obvious sources like smoking or chewing tobacco, however these are not the only administration methods. Secondhand smoke exposure can affect even those who choose not to expose themselves to tobacco, which also opens them to the health issues that come along with tobacco use. Tobacco contains many different compounds, one of which is nicotine (Kataoka, 2009). Although nicotine is the most abundant compound in tobacco, it does not stay in the body for long periods of time. Nicotine metabolizes into cotinine which is more …

Assessing The Detectability Of Cannabinoid Analogs (Delta-8 Thc, Delta-10 Thc And Cbd) And Their Major Metabolites In Six Commercial Cannabinoid Urine Screening Kits, Ashley A. Pokhai

Master of Science in Forensic Science Directed Research Projects

Background:

There has been an exponential surge in the presence and use of cannabinoids since the federal legalization of hemp (Agricultural Improvement Act of 2018), which involves the legalization of medical use, adult recreational use, and decriminalization of cannabis in several states. This growth is not only attributed to ∆9-tetrahydrocannabinol (∆9-THC) and cannabidiol (CBD), the most abundant phytocannabinoid components of cannabis and hemp, respectively, but with many other emerging THC analogs. Structurally, these analogs are similar to ∆9-THC, yet very little information is available about their potency and even less information is available regarding their detectability using commercially available cannabinoid …

A Low-Cost Machine Learning Based Network Intrusion Detection System With Data Privacy Preservation, Jyoti Fakirah, Lauhim Mahfuz Zishan, Roshni Mooruth, Michael L. Johnstone, Wencheng Yang

Annual ADFSL Conference on Digital Forensics, Security and Law

Network intrusion is a well-studied area of cyber security. Current machine learning-based network intrusion detection systems (NIDSs) monitor network data and the patterns within those data but at the cost of presenting significant issues in terms of privacy violations which may threaten end-user privacy. Therefore, to mitigate risk and preserve a balance between security and privacy, it is imperative to protect user privacy with respect to intrusion data. Moreover, cost is a driver of a machine learning-based NIDS because such systems are increasingly being deployed on resource-limited edge devices. To solve these issues, in this paper we propose a NIDS …

Human-Controlled Fuzzing With Afl, Maxim Grishin, Igor Korkin, Phd

Annual ADFSL Conference on Digital Forensics, Security and Law

Fuzzing techniques are applied to reveal different types of bugs and vulnerabilities. American Fuzzy Lop (AFL) is a free most popular software fuzzer used by many other fuzzing frameworks. AFL supports autonomous mode of operation that uses the previous step output into the next step, as a result fuzzer spends a lot of time analyzing minor code sections. By making fuzzing process more focused and human controlled security expert can save time and find more bugs in less time. We designed a new module that can fuzz only the specified functions. As a result, the chosen ones will be inspected …

The Amorphous Nature Of Hackers: An Exploratory Study, Kento Yasuhara, Daniel Walnycky, Ibrahim Baggili, Ahmed Alhishwan

Annual ADFSL Conference on Digital Forensics, Security and Law

In this work, we aim to better understand outsider perspectives of the hacker community through a series of situation based survey questions. By doing this, we hope to gain insight into the overall reputation of hackers from participants in a wide range of technical and non-technical backgrounds. This is important to digital forensics since convicted hackers will be tried by people, each with their own perception of who hackers are. Do cyber crimes and national security issues negatively affect people’s perceptions of hackers? Does hacktivism and information warfare positively affect people’s perception of hackers? Do individual personality factors affect one’s …

Death Scene Insect Succession In Nebraska: A Guidebook, Erin Bauer, Larry Barksdale, Emma Sidel, Justine Laviolette

Department of Entomology: Faculty Publications

Insect behavior can be helpful to law enforcement in determining time of death, manner of death, location, and environment related to human or other animal victims found at a death scene. They may also provide clues about other aspects associated with an investigation (i.e., fly specks, suspect DNA). The study of how insects and related arthropods can aid in legal investigations is known as forensic entomology. Although this includes both civil applications, such as urban (i.e., maggots in mortuaries or insect structural damage) or stored product (i.e., illness from food contamination) entomology, this manual focuses on criminal applications, such as …

Internal Validation Of Mastr™ Probabilistic Genotyping Software For The Interpretation Of 2–5 Person Mixed Dna Profiles, Michael S. Adamowicz, Taylor N. Rambo, Jennifer L. Clarke

Forensic Science: Faculty Publications

Mixed human deoxyribonucleic acid (DNA) samples present one of the most challenging pieces of evidence that a forensic analyst can encounter. When multiple contributors, stochastic amplification, and allele drop-out further complicate the mixture profile, interpretation by hand becomes unreliable and statistical analysis problematic. Probabilistic genotyping software has provided a tool to address complex mixture interpretation and provide likelihood ratios for defined sets of propositions. The MaSTR™ software is a fully continuous probabilistic system that considers a wide range of STR profile data to provide likelihood ratios on DNA mixtures. Mixtures with two to five contributors and a range of component …

The Further Erosion Of Investor Protection: Expanded Exemptions, Spac Mergers, And Direct Listings, Andrew F. Tuch, Joel Seligman

Scholarship@WashULaw

This Article examines the decades-long decline of investor protections enshrined in the Securities Act of 1933, most notably Section 11, which imposes near strict liability on corporate insiders and certain secondary actors, primarily underwriters. The provision, the most potent in the federal securities regulatory arsenal, popularized the concept of outside gatekeepers and transformed practices in securities offerings, making due diligence a byword for careful investigation of facts whether required by legal process or otherwise. The measures required by Section 11 restored confidence in US capital markets in the wake of the Great Depression and have been instrumental in these markets’ …

Lemonade: A Racial Justice Reframing Of The Roberts Court’S Criminal Jurisprudence, Daniel S. Harawa

Scholarship@WashULaw

The saying goes, when life gives you lemons, make lemonade. When it comes to the Supreme Court’s criminal jurisprudence and its relationship to racial (in)equity, progressive scholars often focus on the tartness of the lemons. In particular, they have studied how the Court often ignores race in its criminal decisions, a move that in turn reifies a racially subordinating criminalization system.

However, the Court has recently issued a series of decisions addressing racism in the criminal legal system: Buck v. Davis, Peña-Rodriguez v. Colorado, Timbs v. Indiana, Flowers v. Mississippi, and
Ramos v. Louisiana. On their face, the cases teach …

Schoolhouse Rap, Andrea L. Dennis

Scholarly Works

Rap on Trial, the treatment of rap music as evidence in the American criminal legal process, is well-documented and increasingly scrutinised. Research has shown that – with little restraint – police, prosecutors, probation officers and judges use rap lyrics to investigate, prosecute and punish individuals. Less noticed is that a similar phenomenon is occurring in the American K–12 educational system, which disciplines school-age youth who participate in rap culture and sometimes refers them to the juvenile or criminal legal systems for additional punishment. This article describes and analyses a small set of identified cases of this scenario, demonstrating that rap …

Where There's A Will There's A Way: Examining The Possible Impacts Of The Covid-19 Pandemic On Incidents Of Mass Violence In The Usa, Gordon A. Crews, Garrison A. Crews, Samantha Leigh Crews

Criminal Justice Faculty Publications

“Where there’s a will there’s a way” is a proverb that simply means if someone is determined to do something, he or she will find a way to accomplish it regardless of obstacles. Unfortunately, this is very true for those who wish to commit acts of violence wish to commit acts of violence. The purpose of this chapter is to examine the possible impacts of the COVID-19 pandemic on incidents of mass and multiple victim violence in the US. More specifically, what impact did efforts such as stay-athome/shelter-in-place orders, telecommuting options for workers, school closures, cancellation of large public events, …

Remarks, Andrea L. Dennis

Scholarly Works

Over the course of one week, the Michigan Journal of Law Reform presented its annual Symposium, this year titled Reimagining Police Surveillance: Protecting Activism and Ending Technologies of Oppression. During this week, the Journal explored complicated questions surrounding the expansion of police surveillance technologies, including how police and federal agencies utilize their extensive resources to identify and surveil public protest, the ways in which technology employed by police is often flawed and disparately impacts people of color, and potential reforms of police surveillance technology. Before delving into these complicated questions, I presented remarks on the history of police surveillance in …

News Treatment Of The Supreme Court: Language Selection, Ideological Directions, And Public Support, Alexander Denison

Theses and Dissertations--Political Science

In an increasingly diverse media landscape, how much of the ideological trends seen in current news reporting affect coverage of the U.S. Supreme Court? This work examines two different aspects of the Court's activities, their decisions and the confirmation hearings of Court nominees, analyzing what factors, if any, lead to differences in coverage language. Finally, through the use of a survey experiment, I analyze whether these differences in language, in combination with positive symbolic imagery, affect attitudes toward the institution. This work provides a novel consideration of whether the Court is subject to the same ideological slant found in coverage …

Fines, Fees, Race, And Socioeconomic Disadvantage, Joshua D. Houy

Dissertations and Theses

Fines and fees for legal violations finance American criminal justice systems but often at a severe cost to those incurring fines and fees. While fines and fees are a long-standing feature of the United States criminal justice system, the use of fines and fees recently captured attention of scholars in the wake of questions prompted by recent social, political, and legal developments. The central question is: What, if any, association is there between race, socioeconomic disadvantage, and county fine and fee issuance? The main hypothesis is: Fine and fee issuance of the most populous counties positively and significantly associate with …

Analysis Of Forensically Relevant Evidence Using Electrochemistry, Spectroscopy, And Mass Spectrometry Tools, Colby Edward Ott

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forensic science relies on the use of multiple techniques in the assessment of evidence to increase the accuracy and reliability of the results. However, with the rapidly changing drug landscape due to the introduction of novel psychoactive substances, many traditional screening methods are no longer sensitive or selective enough for use. Additionally, many screening methods such as chemical color tests are prone to false positive and negative results and are subjective. Therefore, the goal of this dissertation was to develop a novel analytical scheme that can provide a more efficient, rapid, and sensitive method that will facilitate adoption in the …

Grassroots Approach Of Embracing Survivors To Inform The Fight Against Human Trafficking, Christina S. Bentch, Gordon A. Crews

Criminal Justice Faculty Publications

When human trafficking survivors work as “informants and advocates,” they keep systems trying to end this horrific crime honest and open to receiving new input. Grassroots approaches can inform many of the systems survivors pass through to improve or add to policies and procedures. Survivors can reveal new dynamics of transnational criminal networks and the development of new indicators and typologies in several systems. The systems discussed in this chapter will highlight the benefits and barriers to approaching an anti-trafficking agenda through a systems theory lens. The authors suggest ways to amend the limitations of these current systems by utilizing …

Criminal Law Exceptionalism, Benjamin Levin

Scholarship@WashULaw

For over half a century, U.S. prison populations have ballooned and criminal codes have expanded. In recent years, a growing awareness of mass incarceration and the harms of criminal law across lines of race and class has led to a backlash of anti-carceral commentary and social movement energy. Academics and activists have adopted a critical posture, offering not only small-bore reforms, but full-fledged arguments for the abolition of prisons, police, and criminal legal institutions. Where criminal law was once embraced by commentators as a catchall solution to social problems, increasingly it is being rejected, or at least questioned. Instead of …

Criminal Justice Expertise, Benjamin Levin

Scholarship@WashULaw

For decades, commentators have adopted a story of mass incarceration’s rise as caused by “punitive populism.” Growing prison populations, expanding criminal codes, and raced and classed disparities in enforcement result from “pathological politics”: voters and politicians act in a vicious feedback loop, driving more criminal law and punishment. The criminal system’s problems are political. But how should society solve these political problems? Scholars often identify two kinds of approaches: (1) the technocratic, which seeks to wrest power from irrational and punitive voters, replacing electoral politics with agencies and commissions; and (2) the democratic, which treats criminal policy as insufficiently responsive …

Victims’ Rights Revisited, Benjamin Levin

Scholarship@WashULaw

This Essay responds to Bennett Capers's article, "Against Prosecutors." I offer four critiques of Capers’s proposal to bring back private prosecutions: (A) that shifting power to victims still involves shifting power to the carceral state and away from defendants; (B) that defining the class of victims will pose numerous problems; C) that privatizing prosecution reinforces a troubling impulse to treat social problems at the individual level; and (D) broadly, that these critiques suggest that Capers has traded the pathologies of “public” law for the pathologies of “private” law. Further, I argue that the article reflects a new, left-leaning vision of …