Open Access. Powered by Scholars. Published by Universities.®
Social and Behavioral Sciences Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Education (2)
- Exercise (2)
- General practice (2)
- Mass (2)
- Nursing history (2)
-
- Older adults (2)
- Past (2)
- Physical activity (2)
- (fyrom/albania) (1)
- (ptme) (1)
- 13c (1)
- 630 (1)
- Abdominal obesity (1)
- Acceleration (1)
- Accelerometry (1)
- Accurate (1)
- Action spectroscopy (1)
- Adults (1)
- Age-depth (1)
- Alternate insemination (1)
- Amino acids (1)
- Anaesthetists (1)
- Analysis (1)
- Antarctica (1)
- Antibiotics (1)
- Artificial insemination (1)
- Asian Indians (1)
- Assessment (1)
- Associated (1)
- Atmospheric and oceanic circulation (1)
Articles 31 - 60 of 438
Full-Text Articles in Social and Behavioral Sciences
Distinct Partitioning Of Als Associated Tdp-43, Fus And Sod1 Mutants Into Cellular Inclusions, Natalie E. Farrawell, Isabella Lambert-Smith, Sadaf T. Warraich, Ian P. Blair, Darren Saunders, Danny M. Danny, Justin J. Yerbury
Distinct Partitioning Of Als Associated Tdp-43, Fus And Sod1 Mutants Into Cellular Inclusions, Natalie E. Farrawell, Isabella Lambert-Smith, Sadaf T. Warraich, Ian P. Blair, Darren Saunders, Danny M. Danny, Justin J. Yerbury
Faculty of Science, Medicine and Health - Papers: part A
Amyotrophic lateral sclerosis is a rapidly progressing neurodegenerative disease associated with protein misfolding and aggregation. Most cases are characterized by TDP-43 positive inclusions, while a minority of familial ALS cases are instead FUS and SOD1 positive respectively. Cells can generate inclusions of variable type including previously characterized aggresomes, IPOD or JUNQ structures depending on the misfolded protein. SOD1 invariably forms JUNQ inclusions but it remains unclear whether other ALS protein aggregates arise as one of these previously described inclusion types or form unique structures. Here we show that FUS variably partitioned to IPOD, JUNQ or alternate structures, contain a mobile …
Cyclic Phosphine Oxides And Phosphinamides From Di-Grignard Reagents And Phosphonic Dichlorides: Modular Access To Annulated Phospholanes, Aaron M. Gregson, Steven M. Wales, Stephen Bailey, Anthony C. Willis, Paul A. Keller
Cyclic Phosphine Oxides And Phosphinamides From Di-Grignard Reagents And Phosphonic Dichlorides: Modular Access To Annulated Phospholanes, Aaron M. Gregson, Steven M. Wales, Stephen Bailey, Anthony C. Willis, Paul A. Keller
Faculty of Science, Medicine and Health - Papers: part A
The reaction between 1,4-di-Grignard reagents and phosphonous(III) dichlorides is a classical method for the direct synthesis of phospholanes. Reported here is an extension of this approach to the preparation of value-added, annulated phospholane oxides, achieved through the combination of carbocyclic-fused di-Grignard reagents and readily available phosphonic(V) dichlorides. The procedure is amenable to (benz)annulation at both the 2,3- and 3,4-positions of the phospholane ring, and a variety of aliphatic, cyclic and aryl P-electrophiles are tolerated in reasonable to excellent yields.
Antibacterial Properties Of 3-(Phenylsulfonyl)-2-Pyrazinecarbonitrile, Rajmohan Rajamuthiah, Elamparithi Jayamani, Hiwa Majed, Annie L. Conery, Wooseong Kim, Bumsup Kwon, Beth Fuchs, Michael J. Kelso, Frederik M. Ausubel, Eleftherios Mylonakis
Antibacterial Properties Of 3-(Phenylsulfonyl)-2-Pyrazinecarbonitrile, Rajmohan Rajamuthiah, Elamparithi Jayamani, Hiwa Majed, Annie L. Conery, Wooseong Kim, Bumsup Kwon, Beth Fuchs, Michael J. Kelso, Frederik M. Ausubel, Eleftherios Mylonakis
Faculty of Science, Medicine and Health - Papers: part A
The emergence of multidrug-resistant bacterial strains has heightened the need for new antimicrobial agents based on novel chemical scaffolds that are able to circumvent current modes of resistance. We recently developed a whole-animal drug-screening methodology in pursuit of this goal and now report the discovery of 3-(phenylsulfonyl)-2-pyrazinecarbonitrile (PSPC) as a novel antibacterial effective against resistant nosocomial pathogens. The minimum inhibitory concentrations (MIC) of PSPC against Staphylococcus aureus and Enterococcus faecium were 4 μg/mL and 8 μg/mL, respectively, whereas the MICs were higher against the Gram-negative bacteria Klebsiella pneumoniae (64 μg/mL), Acinetobacter baumannii (32 μg/mL), Pseudomonas aeruginosa (>64 μg/mL), and …
A Landscape-Scale, Applied Fire Management Experiment Promotes Recovery Of A Population Of The Threatened Gouldian Finch, Erythrura Gouldiae, In Australia's Tropical Savannas, Sarah Legge, Stephen Garnett, Kimberly L. Maute, Joanne Heathcote, Steve Murphy, John Woinarski, Lee Astheimer
A Landscape-Scale, Applied Fire Management Experiment Promotes Recovery Of A Population Of The Threatened Gouldian Finch, Erythrura Gouldiae, In Australia's Tropical Savannas, Sarah Legge, Stephen Garnett, Kimberly L. Maute, Joanne Heathcote, Steve Murphy, John Woinarski, Lee Astheimer
Faculty of Science, Medicine and Health - Papers: part A
Fire is an integral part of savanna ecology and changes in fire patterns are linked to biodiversity loss in savannas worldwide. In Australia, changed fire regimes are implicated in the contemporary declines of small mammals, riparian species, obligate-seeding plants and grass seed-eating birds. Translating this knowledge into management to recover threatened species has proved elusive. We report here on a landscape-scale experiment carried out by the Australian Wildlife Conservancy (AWC) on Mornington Wildlife Sanctuary in northwest Australia. The experiment was designed to understand the response of a key savanna bird guild to fire, and to use that information to manage …
Building Research Skills To Empower Evidence Based Practitioners, Judy Mullan, Kathryn M. Weston, Warren C. Rich, Shelley P. Crowther, Peter L. Mclennan
Building Research Skills To Empower Evidence Based Practitioners, Judy Mullan, Kathryn M. Weston, Warren C. Rich, Shelley P. Crowther, Peter L. Mclennan
Faculty of Science, Medicine and Health - Papers: part A
Abstract of a presentation at the HERDSA 2015 Conference, 6-9 July, Melbourne, Australia.
Magnesium Sulfate Has Sex-Specific, Dose-Dependent Vasodilator Effects On Preterm Placental Vessels, Clint Gray, Mark Vickers, Rebecca M. Dyson, Clare Reynolds, Mary J. Berry
Magnesium Sulfate Has Sex-Specific, Dose-Dependent Vasodilator Effects On Preterm Placental Vessels, Clint Gray, Mark Vickers, Rebecca M. Dyson, Clare Reynolds, Mary J. Berry
Faculty of Science, Medicine and Health - Papers: part A
Background Women at risk of preterm delivery receive magnesium sulfate (MgSO 4 ) in the pre-delivery phase to reduce their child's risk of neurodevelopmental complications associated with preterm birth. However, the mechanisms underpinning its placental vascular role remain uncertain. Methods The aim of this study was to examine MgSO 4 action on vascular tone in male and female human placental vessels from term and preterm deliveries. Vessels were obtained from placental biopsy following birth at term (37-41 weeks) or preterm gestation (gestation). The vessels were mounted on a pressure myograph, pre-constricted with synthetic endoperoxide prostaglandin PGH 2 (U46619) (0.1-100 μmol/l), …
Engaging Medical Students In The Continuum Of Research: Key Requirements For A 'Total Research Experience', Kathryn M. Weston, Judy Mullan, Peter L. Mclennan, Warren C. Rich, Shelley P. Crowther, John A. Bushnell
Engaging Medical Students In The Continuum Of Research: Key Requirements For A 'Total Research Experience', Kathryn M. Weston, Judy Mullan, Peter L. Mclennan, Warren C. Rich, Shelley P. Crowther, John A. Bushnell
Faculty of Science, Medicine and Health - Papers: part A
Abstract of a presentation at the AMEE 2015 conference, 4-9 September, Glasgow, United Kingdom.
Holocene Floodplain Soils Along The Río Mamoré, Northern Bolivia, And Their Implications For Understanding Inundation And Depositional Patterns In Seasonal Wetland Settings, Jan-Hendrik May, Anna Plotzki, Leonor Rodrigues, Frank Preusser, Heinz Veit
Holocene Floodplain Soils Along The Río Mamoré, Northern Bolivia, And Their Implications For Understanding Inundation And Depositional Patterns In Seasonal Wetland Settings, Jan-Hendrik May, Anna Plotzki, Leonor Rodrigues, Frank Preusser, Heinz Veit
Faculty of Science, Medicine and Health - Papers: part A
The Llanos de Moxos (LM) in the Bolivian Amazon basin host one of the largest seasonally inundated savannah landscapes on Earth. Very little is known of this area with regard to sedimentary dynamics, soil formation, or their relationship to longer-term climatic and hydrological variability in this setting. Here we present a detailed study of three floodplain depositional sequences building up the inundation savannah along the Mamoré River in the central LM. Pleistocene sands and silts (unit 1) are overlain by thick overbank deposits of mostly Holocene age (unit 2) on both sides of the Mamoré River, and underline the importance …
A Pharmacist Integrated Into A General Practice Setting - Quality Improvement Outcomes In The Management Of Anticoagulants, Margaret L. Jordan, Haley Frew, Adele F. Stewart, Judy Mullan
A Pharmacist Integrated Into A General Practice Setting - Quality Improvement Outcomes In The Management Of Anticoagulants, Margaret L. Jordan, Haley Frew, Adele F. Stewart, Judy Mullan
Faculty of Science, Medicine and Health - Papers: part A
Poster presentation at 2015 Primary Health Care Research Conference, Adelaide, Australia, 29-31 July.
An Assessment Of The Appropriate Use Of Medicines In Older Patients With Impaired Kidney Function, In A General Practice Setting, Margaret L. Jordan, Julie M. Blaze, Jeffrey G. Hall, Judy Mullan
An Assessment Of The Appropriate Use Of Medicines In Older Patients With Impaired Kidney Function, In A General Practice Setting, Margaret L. Jordan, Julie M. Blaze, Jeffrey G. Hall, Judy Mullan
Faculty of Science, Medicine and Health - Papers: part A
Poster presentation at 2015 Primary Health Care Research Conference, Adelaide, Australia, 29-31 July.
Patients' Experiences Of A Clinical Pharmacist Integrated Into A General Practice Setting, Margaret L. Jordan, Haley Frew, Adele F. Stewart, Judy Mullan
Patients' Experiences Of A Clinical Pharmacist Integrated Into A General Practice Setting, Margaret L. Jordan, Haley Frew, Adele F. Stewart, Judy Mullan
Faculty of Science, Medicine and Health - Papers: part A
Presentation at 2015 Primary Health Care Research Conference, Adelaide, Australia, 29-31 July.
The Impact Of An Intact Infrapatellar Fat Pad On Outcomes After Total Knee Arthroplasty, Leigh D. White, Nicholas J. Hartnell, Melissa Hennessy, Judy Mullan
The Impact Of An Intact Infrapatellar Fat Pad On Outcomes After Total Knee Arthroplasty, Leigh D. White, Nicholas J. Hartnell, Melissa Hennessy, Judy Mullan
Faculty of Science, Medicine and Health - Papers: part A
Background. The infrapatellar fat pad (IPFP) is currently resected in approximately 88% of Total Knee Arthroplasties (TKAs). We hypothesised that an intact IPFP would improve outcomes after TKA. Methods. Patients with an intact IPFP participated in this cross-sectional study by completing two surveys, at 6 and 12 months after TKA. Both surveys included questions regarding kneeling, with the Oxford Knee Score also included at 12 months. Results. Sixty patients participated in this study. At 6 and 12 months, a similar number of patients were able to kneel, 40 (66.7%) and 43 (71.7%), respectively. Fifteen (25.0%) patients were unable to …
Assessing 5 Years Of Gosat Proxy Xch4 Data And Associated Uncertainties, Robert J. Parker, Hartmut Boesch, K Byckling, A J. Webb, Paul I. Palmer, L Feng, P Bergamaschi, Frédéric Chevallier, Justus Notholt, Nicholas M. Deutscher, Thorsten Warneke, Frank Hase, Ralf Sussmann, S Kawakami, Rigel Kivi, David W. T Griffith, Voltaire A. Velazco
Assessing 5 Years Of Gosat Proxy Xch4 Data And Associated Uncertainties, Robert J. Parker, Hartmut Boesch, K Byckling, A J. Webb, Paul I. Palmer, L Feng, P Bergamaschi, Frédéric Chevallier, Justus Notholt, Nicholas M. Deutscher, Thorsten Warneke, Frank Hase, Ralf Sussmann, S Kawakami, Rigel Kivi, David W. T Griffith, Voltaire A. Velazco
Faculty of Science, Medicine and Health - Papers: part A
We present 5 years of GOSAT XCH4 retrieved using the "proxy" approach. The Proxy XCH4 data are validated against ground-based TCCON observations and are found to be of high quality with a small bias of 4.8 ppb (∼ 0.27 %) and a single-sounding precision of 13.4 ppb (∼ 0.74 %). The station-to-station bias (ameasure of the relative accuracy) is found to be 4.2 ppb. For the first time the XCH4 / XCO2 ratio component of the Proxy retrieval is validated (bias of 0.014 ppb ppm−1 (∼ 0.30 %), single-sounding precision of 0.033 ppb ppm−1 (∼ 0.72 %)). The uncertainty relating …
Are The Three Hydroxyphenyl Radical Isomers Created Equal? - The Role Of The Phenoxy Radical -, Patrick Hemberger, Gabriel Da Silva, Adam J. Trevitt, Thomas Gerber, A Bodi
Are The Three Hydroxyphenyl Radical Isomers Created Equal? - The Role Of The Phenoxy Radical -, Patrick Hemberger, Gabriel Da Silva, Adam J. Trevitt, Thomas Gerber, A Bodi
Faculty of Science, Medicine and Health - Papers: part A
We have investigated the thermal decomposition of the three hydroxyphenyl radicals (˙C6H4OH) in a heated microtubular reactor. Intermediates and products were identified isomer-selectively applying photoion mass-selected threshold photoelectron spectroscopy with vacuum ultraviolet synchrotron radiation. Similarly to the phenoxy radical (C6H5-O˙), hydroxyphenyl decomposition yields cyclopentadienyl (c-C5H5) radicals in a decarbonylation reaction at elevated temperatures. This finding suggests that all hydroxyphenyl isomers first rearrange to form phenoxy species, which subsequently decarbonylate, a mechanism which we also investigate computationally. Meta- and para-radicals were selectively produced and spectroscopically detectable, whereas the ortho isomer could not be traced due to its fast rethermalization and rapid …
Collaboration In General Practice: Working Together To Deliver Clinical Care, Susan Mcinnes, Elizabeth J. Halcomb, Andrew D. Bonney, Kathleen Peters
Collaboration In General Practice: Working Together To Deliver Clinical Care, Susan Mcinnes, Elizabeth J. Halcomb, Andrew D. Bonney, Kathleen Peters
Faculty of Science, Medicine and Health - Papers: part A
Abstract of a poster presented at he 2015 PHC Research Conference, 29-31 July, Adelaide, Australia.
The Australian Primary Care Nursing Workforce 2015, Elizabeth J. Halcomb
The Australian Primary Care Nursing Workforce 2015, Elizabeth J. Halcomb
Faculty of Science, Medicine and Health - Papers: part A
Abstract of a poster presented at he 2015 PHC Research Conference, 29-31 July, Adelaide, Australia.
The Development Of National Practice Standards For Nurses In General Practice, Elizabeth J. Halcomb, Julianne Bryce, Elizabeth Foley, Christine Ashley
The Development Of National Practice Standards For Nurses In General Practice, Elizabeth J. Halcomb, Julianne Bryce, Elizabeth Foley, Christine Ashley
Faculty of Science, Medicine and Health - Papers: part A
Abstract of a paper presented at he 2015 PHC Research Conference, 29-31 July, Adelaide, Australia.
Thermally-Promoted Post-Synthetic Pummerer Chemistry In A Sulfoxide-Functionalized Metal-Organic Framework, Macguire Bryant, Christopher Richardson
Thermally-Promoted Post-Synthetic Pummerer Chemistry In A Sulfoxide-Functionalized Metal-Organic Framework, Macguire Bryant, Christopher Richardson
Faculty of Science, Medicine and Health - Papers: part A
Reported herein is a thermally-promoted, post-synthetic modification of a zinc metal-organic frameworks pore surface. Sulfoxide tag groups are modified by conventional heating via Pummerer-type chemistry and elimination to aldehyde groups. Simultaneously, a minor competing disproportionation reaction occurs, creating sulfide and sulfone tag groups enhancing the complexity of the pores. The thermal process is gentle, with crystallinity fully maintained, and results in a higher surface area and pore volume for the modified framework.
Improving Blood Pressure Control In Primary Care: Feasibility And Impact Of The Impress Intervention, Nicholas Arnold Zwar, Oshana Hermiz, Elizabeth J. Halcomb, Patricia M. Davidson, Thomas Bodenheimer
Improving Blood Pressure Control In Primary Care: Feasibility And Impact Of The Impress Intervention, Nicholas Arnold Zwar, Oshana Hermiz, Elizabeth J. Halcomb, Patricia M. Davidson, Thomas Bodenheimer
Faculty of Science, Medicine and Health - Papers: part A
Abstract of a paper presented at he 2015 PHC Research Conference, 29-31 July, Adelaide, Australia.
Attitudes Of Nonpalliative Care Nurses Towards Palliative Care, Victoria Tait, Megan Higgs, Linda Magann, Joanne Dixon, Jan Maree Davis, Ritin S. Fernandez
Attitudes Of Nonpalliative Care Nurses Towards Palliative Care, Victoria Tait, Megan Higgs, Linda Magann, Joanne Dixon, Jan Maree Davis, Ritin S. Fernandez
Faculty of Science, Medicine and Health - Papers: part A
The quality of palliative care given to terminally ill patients and their family members can be directly impacted by the attitudes that nurses hold towards palliative care. This study aimed to investigate the attitudes of nonpalliative care nurses towards death and dying in the context of palliative care. Nurses working within the medical aged care, cardiology and respiratory wards at two metropolitan teaching hospitals in Sydney completed the Frommelt Attitudes Towards Care of the Dying (FATCOD) scale, an anonymous self-administered questionnaire, and a twelve-item demographic questionnaire. A total of 95 completed surveys were used in the final analysis. The total …
An Integrative Review: Understanding Driving Retirement Decisions For Individuals Living With A Dementia, Catherine L. Andrew, Victoria Traynor, Donald C. Iverson
An Integrative Review: Understanding Driving Retirement Decisions For Individuals Living With A Dementia, Catherine L. Andrew, Victoria Traynor, Donald C. Iverson
Faculty of Science, Medicine and Health - Papers: part A
Aim To synthesise primary research exploring decision making practices used to determine the time to retire from driving for individuals living with a dementia. Background Driving requires complex cognitive and physical skills potentially compromised due to the progressive nature of dementia. Whilst on-road assessments are considered reliable indicators of driving capacity by clinicians, drivers with dementia disagree. Design Integrative literature review informed by Whittemore & Knafl (2005). Data sources Electronic database search of Medline, CINAHL, Web of Science, Google Scholar 1997-2012; and incremental hand search. Review methods Primary studies published in peer reviewed journals were appraised against quality assessment criteria …
Getting Men To Talk About Suicide, Lorna Moxham, Christopher F. Patterson
Getting Men To Talk About Suicide, Lorna Moxham, Christopher F. Patterson
Faculty of Science, Medicine and Health - Papers: part A
It may be hard to get men talking about their mental health, but that is just what we need to do.
Risk Assessment Of Trace Element Pollution In Gymea Bay, Nsw, Australia, Yasir M. Alyazichi, Brian G. Jones, Errol J. Mclean, Hamad Nasser Altalyan, Ali Kareem Mohammed Al-Nasrawi
Risk Assessment Of Trace Element Pollution In Gymea Bay, Nsw, Australia, Yasir M. Alyazichi, Brian G. Jones, Errol J. Mclean, Hamad Nasser Altalyan, Ali Kareem Mohammed Al-Nasrawi
Faculty of Science, Medicine and Health - Papers: part A
The main purpose of this study is to assess the sediment quality and potential ecological risk in marine sediments in Gymea Bay located in south Sydney, Australia. A total of 32 surface sediment samples were collected from the bay. Current track trajectories and velocities have also been measured in the bay. The resultant trace elements were compared with the adverse biological effect values Effect Range Low (ERL) and Effect Range Median (ERM) classifications. The results indicate that the average values of chromium, arsenic, copper, zinc, and lead in surface sediments all reveal low pollution levels and are below ERL and …
If You Can't Engage Them They Won't Attend, Rebekkah Middleton, Lorna Moxham, Dominique R. Parrish
If You Can't Engage Them They Won't Attend, Rebekkah Middleton, Lorna Moxham, Dominique R. Parrish
Faculty of Science, Medicine and Health - Papers: part A
Diabetes is a prevalent and chronic disease both in Australia and internationally, affecting 347 million people worldwide. Estimates suggest diabetes will be the seventh leading cause of death by 2030 (WHO 2015).
Using Recreation To Create A Therapeutic Milieu That Works For Mental Health Consumers, Shane Pegg, Lorna Moxham, Dana J. Perlman, Susan Liersch, Christopher F. Patterson, Renee M. Brighton, Ellie K. Taylor
Using Recreation To Create A Therapeutic Milieu That Works For Mental Health Consumers, Shane Pegg, Lorna Moxham, Dana J. Perlman, Susan Liersch, Christopher F. Patterson, Renee M. Brighton, Ellie K. Taylor
Faculty of Science, Medicine and Health - Papers: part A
Abstract of a presentation
Native Hemiparasite And Light Effects On Photoprotection And Photodamage In A Native Host, Robert M. Cirocco, Melinda J. Waterman, Sharon A. Robinson, Jose M. Facelli, Jennifer R. Watling
Native Hemiparasite And Light Effects On Photoprotection And Photodamage In A Native Host, Robert M. Cirocco, Melinda J. Waterman, Sharon A. Robinson, Jose M. Facelli, Jennifer R. Watling
Faculty of Science, Medicine and Health - Papers: part A
Plants infected with hemiparasites often have lowered rates of photosynthesis, which could make them more susceptible to photodamage. However, it is also possible that infected plants increase their photoprotective capacity by changing their pigment content and/or engagement of the xanthophyll cycle. There are no published studies investigating infection effects on host pigment dynamics and how this relates to host susceptibility to photodamage whether in high (HL) or low light (LL). A glasshouse experiment was conducted where Leptospermum myrsinoides Schltdl. either uninfected or infected with Cassytha pubescens R.Br. was grown in HL or LL and pigment content of both host and …
Emissions From Prescribed Fires In Temperate Forest In South-East Australia: Implications For Carbon Accounting, M Possell, Meaghan Jenkins, Tina L. Bell, Mark A. Adams
Emissions From Prescribed Fires In Temperate Forest In South-East Australia: Implications For Carbon Accounting, M Possell, Meaghan Jenkins, Tina L. Bell, Mark A. Adams
Faculty of Science, Medicine and Health - Papers: part A
We estimated emissions of carbon, as equivalent CO2 (CO2e), from planned fires in four sites in a south-eastern Australian forest. Emission estimates were calculated using measurements of fuel load and carbon content of different fuel types, before and after burning, and determination of fuel-specific emission factors. Median estimates of emissions for the four sites ranged from 20 to 139 Mg CO2e ha−1. Variability in estimates was a consequence of different burning efficiencies of each fuel type from the four sites. Higher emissions resulted from more fine fuel (twigs, decomposing matter, near-surface live and leaf litter) or coarse woody debris (CWD; …
Lipid Peroxidation Is Essential For Α-Synuclein-Induced Cell Death, Plamena R. Angelova, Mathew H. Horrocks, David Klenerman, Sonia Gandhi, Andrey Y. Abramov, Mikhail S. Shchepinov
Lipid Peroxidation Is Essential For Α-Synuclein-Induced Cell Death, Plamena R. Angelova, Mathew H. Horrocks, David Klenerman, Sonia Gandhi, Andrey Y. Abramov, Mikhail S. Shchepinov
Faculty of Science, Medicine and Health - Papers: part A
Parkinson's disease is the second most common neurodegenerative disease and its pathogenesis is closely associated with oxidative stress. Deposition of aggregated α-synuclein (α-Syn) occurs in familial and sporadic forms of Parkinson's disease. Here, we studied the effect of oligomeric α-Syn on one of the major markers of oxidative stress, lipid peroxidation, in primary co-cultures of neurons and astrocytes. We found that oligomeric but not monomeric α-Syn significantly increases the rate of production of reactive oxygen species, subsequently inducing lipid peroxidation in both neurons and astrocytes. Pre-incubation of cells with isotope-reinforced polyunsaturated fatty acids (D-PUFAs) completely prevented the effect of oligomeric …
A Mechanistic Model Of Tau Amyloid Aggregation Based On Direct Observation Of Oligomers, Sarah L. Shammas, Gonzalo A. Garcia, Satish Kumar, Magnus Kjaergaard, Mathew H. Horrocks, Nadia Shivji, Eva Mandelkow, Tuomas P. J Knowles, Eckhard Mandelkow, David Klenerman
A Mechanistic Model Of Tau Amyloid Aggregation Based On Direct Observation Of Oligomers, Sarah L. Shammas, Gonzalo A. Garcia, Satish Kumar, Magnus Kjaergaard, Mathew H. Horrocks, Nadia Shivji, Eva Mandelkow, Tuomas P. J Knowles, Eckhard Mandelkow, David Klenerman
Faculty of Science, Medicine and Health - Papers: part A
Protein aggregation plays a key role in neurodegenerative disease, giving rise to small oligomers that may become cytotoxic to cells. The fundamental microscopic reactions taking place during aggregation, and their rate constants, have been difficult to determine due to lack of suitable methods to identify and follow the low concentration of oligomers over time. Here we use single-molecule fluorescence to study the aggregation of the repeat domain of tau (K18), and two mutant forms linked with familial frontotemporal dementia, the deletion mutant ΔK280 and the point mutant P301L. Our kinetic analysis reveals that aggregation proceeds via monomeric assembly into small …
Diastereoselective Synthesis Of The A-B-C Tricyclic Ring Structure Of Stemocurtisine, Xuan Duc Dau, Anthony C. Willis, Stephen G. Pyne
Diastereoselective Synthesis Of The A-B-C Tricyclic Ring Structure Of Stemocurtisine, Xuan Duc Dau, Anthony C. Willis, Stephen G. Pyne
Faculty of Science, Medicine and Health - Papers: part A
The diastereoselective synthesis of the A-B-C tricyclic ring structure of the Stemona alkaloid stemocurtisine is described. This tricyclic precursor to the natural product was obtained in 19 steps from a known vinyl iodide. Attempts to prepare the C-3a-C-11 ether moiety of this alkaloid through a photochemically induced oxidative cyclization method were unsuccessful because of the cleavage of the A-ring.