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Statistical Methodology To Establish A Benchmark For Evaluating Antimicrobial Resistance Genes Through Real Time Pcr Assay, Enakshy Dutta
Statistical Methodology To Establish A Benchmark For Evaluating Antimicrobial Resistance Genes Through Real Time Pcr Assay, Enakshy Dutta
Department of Statistics: Dissertations, Theses, and Student Work
Novel diagnostic tests are usually compared with gold standard tests for evaluating diagnostic accuracy. For assessing antimicrobial resistance (AMR) to bovine respiratory disease (BRD) pathogens, phenotypic broth microdilution method is used as gold standard (GS). The objective of the thesis is to evaluate the optimal cycle threshold (Ct) generated by real-time polymerase chain reaction (rtPCR) to genes that confer resistance that will translate to the phenotypic classification of AMR. Data from two different methodologies are assessed to identify Ct that will discriminate between resistance (R) and susceptibility (S). First, the receiver operating characteristic (ROC) curve was used to determine the …