Open Access. Powered by Scholars. Published by Universities.®
Statistical, Nonlinear, and Soft Matter Physics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Bessel functions (1)
- Biological Physics (1)
- Biopolymers (1)
- Composite materials (1)
- Conjugate acid-base pairs (1)
-
- Deformation (1)
- Frequency response (1)
- Globular proteins (1)
- Hydrodynamics (1)
- Immunology (1)
- Mechanical properties (1)
- Molecular Spring (1)
- Nanoparticles (1)
- Nonlinear Dynamics (1)
- Nutrient and storage proteins (1)
- Particles (Nuclear physics) (1)
- Peptides and proteins (1)
- Phase transformations (Statistical physics) (1)
- Quantum dots (1)
- Soft Matter (1)
Articles 1 - 4 of 4
Full-Text Articles in Statistical, Nonlinear, and Soft Matter Physics
Small Quantum Dots Conjugated To Nanobodies As Immunofluorescence Probes For Nanometric Microscopy, Yong Wang, En Cai, Tobias Rosenkranz, Pinghua Ge, Kai Wen Teng, Sung Jun Lim, Andrew M. Smith, Hee Jung Chung, Frederick Sachs, William N. Green, Philip Gottlieb, Paul R. Selvin
Small Quantum Dots Conjugated To Nanobodies As Immunofluorescence Probes For Nanometric Microscopy, Yong Wang, En Cai, Tobias Rosenkranz, Pinghua Ge, Kai Wen Teng, Sung Jun Lim, Andrew M. Smith, Hee Jung Chung, Frederick Sachs, William N. Green, Philip Gottlieb, Paul R. Selvin
Physics Faculty Publications and Presentations
Immunofluorescence, a powerful technique to detect specific targets using fluorescently labeled antibodies, has been widely used in both scientific research and clinical diagnostics. The probes should be made with small antibodies and high brightness. We conjugated GFP binding protein (GBP) nanobodies, small single-chain antibodies from llamas, with new ∼7 nm quantum dots. These provide simple and versatile immunofluorescence nanoprobes with nanometer accuracy and resolution. Using the new probes we tracked the walking of individual kinesin motors and measured their 8 nm step sizes; we tracked Piezo1 channels, which are eukaryotic mechanosensitive channels; we also tracked AMPA receptors on living neurons. …
Viscoelastic Transition And Yield Strain Of The Folded Protein, Yong Wang, Giovanni Zocchi
Viscoelastic Transition And Yield Strain Of The Folded Protein, Yong Wang, Giovanni Zocchi
Physics Faculty Publications and Presentations
For proteins, the mechanical properties of the folded state are directly related to function, which generally entails conformational motion. Through sub-Angstrom resolution measurements of the AC mechanical susceptibility of a globular protein we describe a new fundamental materials property of the folded state. For increasing amplitude of the forcing, there is a reversible transition from elastic to viscoelastic response. At fixed frequency, the amplitude of the deformation is piecewise linear in the force, with different slopes in the elastic and viscoelastic regimes. Effectively, the protein softens beyond a yield point defined by this transition. We propose that ligand induced conformational …
Critical Torque For Kink Formation In Double-Stranded Dna, Hao Qu, Yong Wang, Chiao-Yu Tseng, Giovanni Zocchi
Critical Torque For Kink Formation In Double-Stranded Dna, Hao Qu, Yong Wang, Chiao-Yu Tseng, Giovanni Zocchi
Physics Faculty Publications and Presentations
We measure the bending energy of double-stranded DNA in the nonlinear (sharply bent) regime. The measurements are obtained from the melting curves of stressed DNA ring molecules. The nonlinear elastic behavior is captured by a single parameter: the critical torque τc at which the molecule develops a kink. In this regime, the elastic energy is linear in the kink angle. This phenomenology is the same as for the previously reported case of nicked DNA. For the sequences examined, we find τc=31 pN×nm. This critical torque corresponds to a characteristic energy scale (π/2)τc=12 kT room relevant for molecular biology processes associated …
First-Order Amplitude For General State-To-State Transitions In Hydrogen By Projectile Impact, Jack C. Straton
First-Order Amplitude For General State-To-State Transitions In Hydrogen By Projectile Impact, Jack C. Straton
Physics Faculty Publications and Presentations
The closed analytic form for bound-state transitions due to projectile impact is found in the intermediate representation. The coordinate integral is obtained by evaluating the remaining two integrals in the general multicenter integral derived previously [J.C. Straton, Phys. Rev. A 41, 71 (1990)]. Evaluating the remaining time integral depends upon relating a sum of modified Bessel functions of the second kind KN+1/2(z) to a simple polynomial in 1/z. The results of Van Den Bos and De Heer [Physica 34, 333 (1967)] are shown to be missing a phase factor of (-i)(l′+l)