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Development Of A Two-Photon Imaging System, Jesseca Hollenbaugh
Development Of A Two-Photon Imaging System, Jesseca Hollenbaugh
University Honors Theses
The objective of this project was to convert a Sarastro 2000 confocal laser scanning microscope (CLSM) into a system capable of far-field two-photon excitation (TPE) imaging for the use of the PSU Biology department. TPE microscopy operates on the ability of fluorophores to accept two photons each with half the energy of a desired transition in a single quantum event via a virtual energy state and then emit a higher energy photon upon relaxation. This is preferable to single-photon excitation (SPE) imaging due to lower photon imaging, causing less damage to delicate biological samples, as well as the inherent localization …