Oceanography Commons^™

Drivers Of Phytoplankton Bloom Interannual Variability In The Amundsen And Pine Island Polynyas, Guillaume Liniger, Delphine Lannuzel, Sébastien Moreau, Michael S. Dinniman, Peter G. Stutton

CCPO Publications

The Amundsen Sea Embayment (ASE) experiences both the highest ice shelf melt rates and the highest biological productivity in West Antarctica. Using 19 years of satellite data and modelling output, we investigate the long-term influence of environmental factors on the phytoplankton bloom in the Amundsen Sea (ASP) and Pine Island (PIP) polynyas. We test the prevailing hypothesis that changes in ice shelf melt rate could drive interannual variability in the polynyas' surface chlorophyll-a (chl a) and Net Primary Productivity (NPP). We find that the interannual variability and long-term change in glacial meltwater may play an important role in …

The Effects Of Varying Temperatures And Food Availability On The Reproductive Output Of The Acorn Barnacle: Chthamalus Fissus, Karina L. Rog

McNair Summer Research Program

Climate change is predicted to intensify ocean warming and the reduction in phytoplankton abundance, potentially disrupting reproductive processes in filter-feeding intertidal invertebrates. This study investigated the combined effects of water temperature increases and food availability on the reproductive output on Chthamalus fissus, a dominant intertidal barnacle species found along the Southern California coastline. Using a controlled laboratory experiment, adult barnacles collected from the nearshore rocky intertidal habitat in La Jolla, California, were exposed to two temperature regimes (ambient water temperature ~ 18°C and elevated water temperature ~ 20-21°C) and two food concentration treatments (low and high phytoplankton and rotifer …

Organic Carbon Decay Mediated By A Mesopelagic Microbial Community: The Relevance Of Carbon Pools And Broad Biochemical Composition, Noah J. Craft, Alexander B. Bochdansky

OES Faculty Publications

The mesopelagic zone is a site of strong microbially driven particle attenuation with depth and thus plays a crucial role in controlling the transfer efficiency of the ocean's biological pump. However, little quantitative information exists on the dependency of decay processes on the source material. Here we followed the decay of ¹⁴C-labeled dead particulate organic carbon (POC) and dissolved organic carbon (DOC) from three different phytoplankton species, and two incubations of live diatoms, in mesopelagic water over 3 months. Commonly used first-order kinetics failed to adequately describe the decay of organic material as rate constants varied from day to day. …

Metabarcoding-Biomass Relationships And Internal Standards For Quantitative Dna Metabarcoding, Frances Webber

Open Access Master's Theses

DNA metabarcoding has been widely adopted due to its power in investigating the composition of protistan communities, accurately identifying even small and cryptic taxa. However, the compositional nature of DNA metabarcoding results, and the distortion of natural abundances due to variation of the often-used 18S rRNA gene copy number over several orders of magnitude, renders these results semi-quantitative at best, limiting the application of DNA metabarcoding to ecological study. Here, we constructed mock communities from uni-algal cultures of taxonomically diverse phytoplankton and conducted metabarcoding using multiple primer sets and both DNA and cDNA as PCR templates. For each culture, chlorophyll …

Seasonal Variability Of Surface Ocean Carbon Uptake And Chlorophyll-A Concentration In The West Antarctic Peninsula Over Two Decades, Jessica S. Turner, David R. Munro, Amanda Fay, Sharon Stammerjohn, Heather Kim, Oscar Schofield, Heidi Dierssen

OES Faculty Publications

The Southern Ocean plays a vital role in global CO₂ uptake, but the magnitude and even the sign of the flux remain uncertain, and the influence of phytoplankton phenology is underexplored. This study focuses on the West Antarctic Peninsula, a region experiencing rapid climate change, to examine shifts in seasonal carbon uptake. Using 20 years of in situ air-sea CO₂ flux and satellite-derived Chlorophyll-a, we observe that the seasonal cycles of both air-sea CO₂ flux and Chlorophyll-a intensify poleward. The amplitude of the seasonal cycle of the non-thermal component of surface ocean pCO₂ increases with increasing latitude, while the amplitude …

Assessing The Gut Content Of Styela Plicata: A Benthic Filter Feeder In The Indian River Lagoon, Honor Lily Triumph Edmands

Theses and Dissertations

The Indian River Lagoon (IRL) has experienced declining water quality due to human activities involving nutrient input, leading to Harmful Algal Blooms (HABs). The Living Docks program affixes oyster mats to dock pilings to promote the settlement and recruitment of benthic filter feeders for water quality improvement. However, questions remain about filtering efficiencies of organisms that make up the mat communities, and whether organisms remove harmful species from the IRL. The pleated sea squirt, Styela plicata, is a common oyster mat resident. The goal of this thesis was to investigate what phytoplankton and bacteria S. plicata consumes, and if …

Interactive Effects Of Co2, Temperature, And Nitrate Limitation On The Growth And Physiology Of Marine Cyanobacterium Synechococcus Sp. Ccmp 1334, Alyssa K. Sharbaugh

LSU Master's Theses

The marine cyanobacterium Synechococcus sp. CCMP 1334 was grown in a continuous culture system on a 12:12 h light:dark cycle at all combinations of low and high pCO2 (400 and 1000 ppmv, respectively), nitrate availability (nitrate-limited and nutrient-replete conditions), and temperatures of 21°C, 24°C, 28°C, 32°C, and 35°C. The maximum median nutrient-replete growth rate was ~1.15 d−1 at 32 –35°C. Median growth rates at 1000 ppmv pCO2 were higher than those at 400 ppmv at all temperatures, but most of the differences were statistically insignificant. Carbon:nitrogen ratios were independent of pCO2 at a fixed relative growth rate but decreased with …

Submarine Groundwater Discharge As A Major Nutrient Source In River-Fed Vs. Tidally Dominated Estuaries, Stephanie J. Wilson, Joseph J. Tamborski, Bongkeun Song, Peter Bernhardt, Margaret R. Mulholland

OES Faculty Publications

The tidal tributaries of the lower Chesapeake Bay experience seasonally recurring harmful algal blooms and the significance of submarine groundwater discharge (SGD) as a nutrient vector is largely unknown. Here, we determined seasonal SGD nutrient loads in two tributaries with contrasting hydrodynamic conditions, river-fed (York River) vs. tidally dominated (Lafayette River). Radon surveys were performed in each river to quantify SGD at the embayment-scale during spring and fall 2021. Total SGD was determined from a ²²²Rn mass balance and Monte Carlo simulations. Submarine groundwater discharge rates differed by a factor of two during spring (Lafayette = 11 ± 17 cm …

Determining The Refractive Index Of Phytoplankton Via Direct Measurement Of Cellular Structures, Michael Kamowski

Master's Theses

The refractive index of phytoplankton has never been directly measured before despite its importance towards studying the optical characteristics of marine particles. Previous attempts to measure it have been done through indirect methods. While these methods have proven useful, they contain assumptions about the particles morphology and composition that cause for uncertainty with the measurements. Through the use of the 3D Cell Explorer, a high precision holotomographic microscope, the RI of phytoplankton can be directly measured for the first time. With volume measurements, the phytoplankton were found to not display a single bulk RI, instead the bulk RI was different …

Nitrite Cycling In The Primary Nitrite Maxima Of The Eastern Tropical North Pacific, Nicole M. Travis, Colette L. Kelly, Margaret R. Mulholland, Karen L. Casciotti

OES Faculty Publications

The primary nitrite maximum (PNM) is a ubiquitous feature of the upper ocean, where nitrite accumulates in a sharp peak at the base of the euphotic zone. This feature is situated where many chemical and hydrographic properties have strong gradients and the activities of several microbial processes overlap. Near the PNM, four major microbial processes are active in nitrite cycling: ammonia oxidation, nitrite oxidation, nitrate reduction and nitrite uptake. The first two processes are mediated by the nitrifying archaeal/bacterial community, while the second two processes are primarily conducted by phytoplankton. The overlapping spatial habitats and substrate requirements for these microbes …

The Importance Of Winter Dinoflagellate Blooms In Chesapeake Bay— A Missing Link In Bay Productivity, Nicole C. Millette, Sophie Clayton, Margaret R. Mulholland, Leah Gibala-Smith, Michael Lane

OES Faculty Publications

It is widely assumed that phytoplankton abundance and productivity decline during temperate winters because of low irradiance and temperatures. However, winter phytoplankton blooms commonly occur in temperate estuaries, but they are often undocumented because of reduced water quality monitoring in winter. The small body of in situ work that has been done on winter blooms suggests they can be of enormous consequence to ecosystems. However, because monitoring is often reduced or stopped altogether during winter, it is unclear how widespread these blooms are or how long they can last. We analyzed an over 30-year record of monthly phytoplankton monitoring samples …

Potential Interactions Between Diatoms And Bacteria Are Shaped By Trace Element Gradients In The Southern Ocean, Alexa R. Sterling, Laura Z. Holland, Randelle M. Bundy, Shannon M. Burns, Kristen N. Buck, P. Dreux Chappell, Bethany D. Jenkins

OES Faculty Publications

The growth of diatoms in the Southern Ocean, especially the region surrounding the West Antarctic Peninsula, is frequently constrained by low dissolved iron and other trace metal concentrations. This challenge may be overcome by mutualisms between diatoms and co-occurring associated bacteria, in which diatoms produce organic carbon as a substrate for bacterial growth, and bacteria produce siderophores, metal-binding ligands that can supply diatoms with metals upon uptake as well as other useful secondary compounds for diatom growth like vitamins. To examine the relationships between diatoms and bacteria in the plankton (diatom) size class (> 3 mu m), we sampled both …

Atmospheric Input And Seasonal Inventory Of Dissolved Iron In The Sargasso Sea: Implications For Iron Dynamics In Surface Waters Of The Subtropical Ocean, Peter N. Sedwick, Bettina M. Sohst, K. N. Buck, S. Caprara, R. J. Johnson, D. C. Ohnemus, L. E. Sofen, A. Tagliabue, B. S. Twining, Tara E. Williams

OES Faculty Publications

Constraining the role of dust deposition in regulating the concentration of the essential micronutrient iron in surface ocean waters requires knowledge of the flux of seawater-soluble iron in aerosols and the replacement time of dissolved iron (DFe) in the euphotic zone. Here we estimate these quantities using seasonally resolved DFe data from the Bermuda Atlantic Time-series Study region and weekly-scale measurements of iron in aerosols and rain from Bermuda during 2019. In response to seasonal changes in vertical mixing, primary production and dust deposition, surface DFe concentrations vary from ∼0.2 nM in early spring to >1 nM in late summer, …

A Global Comparison Of Marine Chlorophyll Variability Observed In Eulerian And Lagrangian Perspectives, Angela M. Kuhn, Matthew Mazloff, Stephanie Dutkiewicz, Oliver Jahn, Sophie Clayton, Tatiana Rynearson, Andrew D. Barton

OES Faculty Publications

The California Current System is a diatom‐dominated region characterized by seasonal coastal upwelling and additional elevated mesoscale activity. Cyclonic mesoscale eddies in the region trap productive coastal waters with their planktonic communities and transport them offshore with limited interaction with surrounding waters, effectively acting as natural mesocosms, where phytoplankton populations undergo ecological succession as eddies age. This study examines diatom community composition within two mesoscale cyclonic eddies that formed in the same region of the California Current System 2 months apart and in the California Current waters surrounding them. The diatom communities were analyzed in the context of shifting environmental …

The Southern Ocean Ecosystem Affects The Entire World, Eugene J. Murphy, Nadine M. Johnston, Eileen E. Hofmann, Richard A. Phillips, Jennifer E. Jackson, Andrew J. Constable

CCPO Publications

The Southern Ocean, which flows around the Antarctic continent, is home to vast numbers of unique and remarkable animals, including penguins, albatrosses, petrels, seals, and whales. The ocean bursts into life every spring, fueling a summer feeding and breeding frenzy. During the dark winter months, there is little food and life is very harsh. Human activities such as fishing and pollution are affecting this ecosystem, as is climate change. These ecosystem changes matter beyond the Southern Ocean! Ocean currents carry nutrients and organisms into and out of the Southern Ocean. Many marine mammals and seabirds swim or fly in and …

Imaging Technologies Build Capacity And Accessibility In Phytoplankton Species Identification Expertise For Research And Monitoring: Lessons Learned During The Covid-19 Pandemic, Sophie Clayton, Leah Gibala-Smith, Kathryn Mogatas, Chanel Flores-Vargas, Kayla Marciniak, Maci Wigginton, Margaret R. Mulholland

OES Faculty Publications

As primary producers, phytoplankton play an integral role in global biogeochemical cycles through their production of oxygen and fixation of carbon. They also provide significant ecosystem services, by supporting secondary production and fisheries. Phytoplankton biomass and diversity have been identified by the Global Ocean Observing System (GOOS) as Essential Ocean Variables (EOVs), properties that need to be monitored to better understand and predict the ocean system. Phytoplankton identification and enumeration relies on the skills and expertise of highly trained taxonomic analysts. The training of new taxonomic analysts is intensive and requires months to years of supervised training before an analyst …

Microplankton Dynamics In The River-Dominated Mississippi Bight, Adam D. Boyette

Dissertations

The Mississippi Bight (MSB) is a river-dominated continental margin influenced by multiple large river systems, including the Mississippi River, Alabama and Tombigbee rivers via Mobile Bay, and numerous smaller rivers, creeks, and bayous. This is part of a biologically-rich ecosystem that supports the second largest fishery industry by volume in the United States. Despite our understanding of the linkages between primary production with higher trophic levels, there remains limited studies quantifying these trophic interactions in this system. Microplankton (µm) community dynamics and trophic connectivity between primary producers and heterotrophic protists represent a critical nexus influencing overall biological productivity in this …

Alexandrium In The Arctic: Are Harmful Algae Spreading As The Arctic Warms?, Sveinn Einarsson, Kate Lowry, Robert Pickart, Karin Ashjian, P. Dreux Chappell

College of Sciences Posters

Alexandrium tamerense is a well-studied dinoflagellate known for its ability to produce the neurotoxin that causes paralytic shellfish poisoning. Until 1970 Alexandrium tamerense was only found in Europe, North America, and Japan but has been increasingly found all over the globe. Alexandrium is characteristically found in temperate and subtropical regions and as the Arctic warms, there is considerable concern that it may be expanding into the Arctic. We found Alexandrium tamerense during a research expedition to the Alaskan Beaufort Sea shelf to study upwelling. Upwelling events are known to support seasonal blooms of phytoplankton, which are important primary producers at …

Developing A Method To Track Marine Snow Aggregation Through Individual Collisions Using Stereoscopic Imaging, Riley Henning

Theses

The aggregation of individual phytoplankton into marine snow allows particles to sink more quickly, thus resulting in the transport of particulate organic carbon from surface waters to the deep ocean. Aggregate formation has previously been measured in experiments indirectly by quantifying how particle size or particle concentration changes over time. Here, I present my thesis in which I applied high-resolution imaging to quantify aggregate formation using two different methodologies.

We conducted experiments to investigate aggregate formation using stereoscopic imaging, tracking individual particles directly in a 3D volume. Phytoplankton cultures were rolled in cylindrical tanks and imaged by two cameras illuminated …

Synoptic Mesoscale To Basin Scale Variability In Biological Productivity And Chlorophyll In The Kuroshio Extension Region, Sophie Clayton, Hilary I. Palevsky, Luanne Thompson, Paul D. Quay

OES Faculty Publications

The Kuroshio current separates from the Japanese coast to become the eastward flowing Kuroshio Extension (KE) characterized by a strong latitudinal density front, high levels of mesoscale (eddy) energy, and high chlorophyll a (Chl). While satellite measurements of Chl show evidence of the impact of mesoscale eddies on the standing stock of phytoplankton, there have been very limited synoptic, spatially resolved in situ estimates of productivity in this region. Here, we present underway measurements of oxygen/argon supersaturation (ΔO2/Ar), a tracer of net biological productivity, for the KE made in spring, summer, and early autumn. We find large seasonal differences in …

Taxonomic And Nutrient Controls On Phytoplankton Iron Quotas In The Ocean, Benjamin S. Twining, Olga Antipova, P. Dreux Chappell, Natalie R. Cohen, Jeremy E. Jacquot, Elizabeth L. Mann, Adrian Marchetti, Daniel C. Ohnemus, Sara Rauschenberg, Alessandro Tagliabue

OES Faculty Publications

Phytoplankton iron contents (i.e., quotas) directly link biogeochemical cycles of iron and carbon and drive patterns of nutrient limitation, recycling, and export. Ocean biogeochemical models typically assume that iron quotas are either static or controlled by dissolved iron availability. We measured iron quotas in phytoplankton communities across nutrient gradients in the Pacific Ocean and found that quotas diverged significantly in taxon‐specific ways from laboratory‐derived predictions. Iron quotas varied 40‐fold across nutrient gradients, and nitrogen‐limitation allowed diatoms to accumulate fivefold more iron than co‐occurring flagellates even under low iron availability. Modeling indicates such “luxury” uptake is common in large regions of …

Water Quality Assessment In The Occurrence Of Acanthaster Spp. (Crown-Of-Thorns Starfish, Cots) On Coral Reefs In Menjangan Island, Bali, Indonesia, Novia Arinda Pradisty, Eghbert Elvan Ampou, Rizki Hanintyo

Makara Journal of Science

Aquatic stressors are known to cause biological impairment that can result in biodiversity loss in several Marine Protected Areas (MPA), including in Menjangan Island, West Bali National Park. The objectives of this study were to correlate the changes in water quality parameters with the biological effect of Crown-of-Thorns starfish (CoTS) population dynamics and to assess the most applicable parameters for continuous environmental monitoring. Field observations and surveys were conducted three times in 2017, during the wet season, dry season and season transition, in order to assess the effect of seasonal variability. Redundancy Analysis (RDA) was performed to determine the relationship …

Phytoplankton And Carbon Dynamics In The Estuarine-Coastal Waters Of The Northern Gulf Of Mexico From Field Data And Ocean Color Remote Sensing, Bingqing Liu

LSU Doctoral Dissertations

In this study, phytoplankton community and carbon dynamics were examined in the optically complex estuarine-coastal regions of the northern Gulf of Mexico (nGOM) from field and satellite ocean color observations. As part of this study, bio-optical ocean color algorithms for i) dissolved organic carbon (DOC), ii) phytoplankton pigment composition, iii) adaptive estimation of Chl a and iv) phytoplankton size fractions were developed to facilitate the study of biogeochemical cycling in the nGOM.

The phytoplankton based algorithms were applied to Sentinel 3A/B-OLCI oean color data to assess phytoplankton community dynamics to extreme river discharge conditions as well as hurricanes in the …

Role Of Coastal Environmental Conditions During Austral Winter On Plankton Community Dynamics And The Occurrence Of Pseudo-Nitzschia Spp. And Domoic Acid In Inhambane Province, Mozambique, Holly Kelchner

LSU Master's Theses

Harmful algal blooms (HABs) are increasing globally in frequency, persistence, and geographic extent. HABs pose a threat to economic stability, and ecosystem and human health. To date no incidences of marine toxins produced by phytoplankton have been recorded in Mozambique, which may be due to the absence of a monitoring program and general awareness of the potential threat. This study is the first documentation of the occurrence of a neurotoxin, domoic acid (DA), produced by the diatom genus Pseudo-nitzschia spp. along the east coast of Africa. The coast of Inhambane Province is a biodiversity hotspot where year-round Rhincodon typus (whale …

Organic Sulfur: A Spatially Variable And Understudied Component Of Marine Organic Matter, Krista Longnecker, Lisa Oswald, Melissa C. Kido Soule, Gregory A. Cutter, Elizabeth B. Kujawinski

OES Faculty Publications

Sulfur (S) is a major heteroatom in organic matter. This project evaluated spatial variability in the concentration and molecular-level composition of organic sulfur along gradients of depth and latitude. We measured the concentration of total organic sulfur (TOS) directly from whole seawater. Our data reveal high variability in organic sulfur, relative to established variability in total organic carbon or nitrogen. The deep ocean contained significant amounts of organic sulfur, and the concentration of TOS in North Atlantic Deep Water (NADW) decreased with increasing age while total organic carbon remained stable. Analysis of dissolved organic matter extracts by ultrahigh resolution mass …

Modeling Iron And Light Controls On The Summer Phaeocystis Antarctica Bloom In The Amundsen Sea Polynya, Hilde Oliver, Pierre St-Laurent, Robert M. Sherrell, Patricia L. Yaeger

CCPO Publications

Of all the Antarctic coastal polynyas, the Amundsen Sea Polynya is the most productive per unit area. Observations from the 2010-2011 Amundsen Sea Polynya International Research Expedition (ASPIRE) revealed that both light and iron can limit the growth of phytoplankton (Phaeocystis antarctica), but how these controls manifest over the bloom season is poorly understood, especially with respect to their climate sensitivity. Using a 1-D biogeochemical model, we examine the influence of light and iron limitation on the phytoplankton bloom and vertical carbon flux at 12 stations representing different bloom stages within the polynya. Model parameters are determined by …

Analysis Of Iron Sources In Antarctic Continental Shelf Waters, Michael S. Dinniman, Pierre St-Laurent, Kevin R. Arrigo, Eileen E. Hofmann, Gert L. Van Dijken

CCPO Publications

Previous studies showed that satellite‐derived estimates of chlorophyll a in coastal polynyas over the Antarctic continental shelf are correlated with the basal melt rate of adjacent ice shelves. A 5‐km resolution ocean/sea ice/ice shelf model of the Southern Ocean is used to examine mechanisms that supply the limiting micronutrient iron to Antarctic continental shelf surface waters. Four sources of dissolved iron are simulated with independent tracers, assumptions about the source iron concentration for each tracer, and an idealized summer biological uptake. Iron from ice shelf melt provides about 6% of the total dissolved iron in surface waters. The contribution from …

Ice Algae Resource Utilization By Benthic Macro- And Megafaunal Communities On The Pacific Arctic Shelf Determined Through Lipid Biomarker Analysis, Chelsea Wegner Koch, Lee W. Cooper, Jacqueline M. Grebmeier, Karen Frey, Thomas A. Brown

Geography

We studied ice algae utilization by benthic fauna from the northern Bering and Chukchi Seas using highly branched isoprenoid (HBI) biomarkers. We assessed whether various food acquisition strategies influence the observed HBI signatures. The proportion of phytoplankton to ice algae-sourced HBIs was determined through the H-Print approach that is presumed to reflect the percentage of sea ice organic carbon (iPOC) incorporated into tissues, relative to phytoplankton organic carbon. Cluster analysis separated 3 groups based on location and feeding strategy that were significantly influenced by annual sea ice persistence. Ice algae utilization was most significant in the northeast Chukchi Sea, where …

Temporal And Spatial Scales Of Correlation In Marine Phytoplankton Communities, A. M. Kuhn, S. Dutkiewicz, O. Jahn, Sophie Clayton, T. A. Rynearson, M. R. Mazloff, A. D. Barton

OES Faculty Publications

Ocean circulation shapes marine phytoplankton communities by setting environmental conditions and dispersing organisms. In addition, processes acting on the water column (e.g., heat fluxes and mixing) affect the community structure by modulating environmental variables that determine in situ growth and loss rates. Understanding the scales over which phytoplankton communities vary in time and space is key to elucidate the relative contributions of local processes and ocean circulation on phytoplankton distributions. Using a global ocean ecosystem model, we quantify temporal and spatial correlation scales for phytoplankton phenotypes with diverse functional traits and cell sizes. Through this analysis, we address these questions: …

Seaflow Data V1, High-Resolution Abundance, Size And Biomass Of Small Phytoplankton In The North Pacific, François Ribalet, Chris Berthiaume, Annette Hynes, Jarred Swalwell, Michael Carlson, Sophie Clayton, Gwenn Hennon, Camille Poirier, Eric Shimabukuro, Angelicque White, E. Virginia Armhurst

OES Faculty Publications

SeaFlow is an underway flow cytometer that provides continuous shipboard observations of the abundance and optical properties of small phytoplankton (μm in equivalent spherical diameter, ESD). Here we present data sets consisting of SeaFlow-based cell abundance, forward light scatter, and pigment fluorescence of individual cells, as well as derived estimates of ESD and cellular carbon content of picophytoplankton, which includes the cyanobacteria Prochlorococcus, Synechococcus and small-sized Crocosphaera (μm ESD), and picophytoplankton and nanophytoplankton (2–5 μm ESD). Data were collected in surface waters (≈5 m depth) from 27 oceanographic cruises carried out in the Northeast Pacific Ocean between 2010 and 2018. …