Oceanography and Atmospheric Sciences and Meteorology Commons^™

Transcriptome Signatures Of Wastewater Effluent Exposure In Larval Zebrafish Vary With Seasonal Mixture Composition In An Effluent-Dominated Stream, Emma B. Meade, Luke R. Iwanowicz, Nicklaus Neureuther, Gregory H. Lefevre, Dana W. Kolpin, Hui Zhi, Shannon M. Meppelink, Rachael F. Lane, Angela Schmoldt, Aurash Mohaimani, Olaf Mueller, Rebecca D. Klaper

United States Geological Survey: Staff Publications

Wastewater treatment plant (WWTP) effluent-dominated streams provide critical habitat for aquatic and terrestrial organisms but also continually expose them to complex mixtures of pharmaceuticals that can potentially impair growth, behavior, and reproduction. Currently, few biomarkers are available that relate to pharmaceutical-specific mechanisms of action. In the experiment reported in this paper, zebrafish (Danio rerio) embryos at two developmental stages were exposed to water samples from three sampling sites (0.1 km upstream of the outfall, at the effluent outfall, and 0.1 km below the outfall) during base-flow conditions from two months (January and May) of a temperate-region effluent-dominated stream …

Sinistral Shear During Middle Jurassic Emplacement Of The Matancilla Plutonic Complex In Northern Chile (25.4◦ S) As Evidence Of Oblique Plate Convergence During The Early Andean Orogeny, S. P. Mavor, J. S. Singleton, G. Heuser, R. Gomila, N. M. Seymour, S. Williams, G. Arancibia

United States Geological Survey: Staff Publications

Arc magmatism in a continental subduction zone facilitates rheological weakening of the rigid upper plate, and can accommodate the partitioned trench-parallel component of oblique subduction into an intra-arc shear zone. We document a shear zone at latitude 25.4^◦ S near Taltal, Chile that was associated with intrusion of the Matancilla Plutonic Complex at ~169 Ma to evaluate intra-arc deformation and possible tectonic plate configurations during this time period. Polyphase folding of Paleozoic metasedimentary rocks is overprinted by mylonitic fabrics that are most extensive in a zone up to 1.4 km wide in the thermal aureole of the granodioritic Matancilla …

Sinistral Shear During Middle Jurassic Emplacement Of The Matancilla Plutonic Complex In Northern Chile (25.4◦ S) As Evidence Of Oblique Plate Convergence During The Early Andean Orogeny, S.P. Mavor, J.S. Singleton, G. Heuser, R Gomila, N.M. Seymour, S. Williams, G. Arancibia

United States Geological Survey: Staff Publications

component of oblique subduction into an intra-arc shear zone. We document a shear zone at latitude 25.4^◦ S near Taltal, Chile that was associated with intrusion of the Matancilla Plutonic Complex at ~169 Ma to evaluate intra-arc deformation and possible tectonic plate configurations during this time period. Polyphase folding of Paleozoic metasedimentary rocks is overprinted by mylonitic fabrics that are most extensive in a zone up to 1.4 km wide in the thermal aureole of the granodioritic Matancilla pluton, where contact metamorphic andalusite porphyroblasts are synkinematic with fabric development. Mylonite in metasedimentary rocks is overprinted by a ~130 Ma …

Discovering Hidden Geothermal Signatures Using Non-Negative Matrix Factorization With Customized K-Means Clustering, V.V. Vesselinov, B. Ahmmed, M.K. Mudunuru, J.D. Pepin, E.R. Burns, D.L. Siler, S. Karra, R.S. Middleton

United States Geological Survey: Staff Publications

Discovery of hidden geothermal resources is challenging. It requires the mining of large datasets with diverse data attributes representing subsurface hydrogeological and geothermal conditions. The commonly used play fairway analysis approach typically incorporates subject-matter expertise to analyze regional data to estimate geothermal characteristics and favorability. We demonstrate an alternative approach based on machine learning (ML) to process a geothermal dataset from southwest New Mexico (SWNM). The study region includes low- and medium-temperature hydrothermal systems. Several of these systems are not well characterized because of insufficient existing data and limited past explorative work. This study discovers hidden patterns and relations in …

The Effects Of Estradiol-17Β On The Sex Reversal, Survival, And Growth Of Green Sunfish Lepomis Cyanellus, Chad N. Teal, Daniel J. Schill, Susan B. Fogelson, Colby M. Roberts, Kevin Fitzsimmons, Javan M. Bauder, William T. Stewart, Scott A. Bonar

United States Geological Survey: Staff Publications

The feminization of green sunfish Lepomis cyanellus could expand their utility as a game fish or aquacultured species by preventing overcrowding and precocious reproduction in stocked systems. Feminization of green sunfish could also help elucidate information on their sex determination system. We report the feminization of green sunfish cohorts via oral administration of estradiol-17β (E2) during early development. A low-dose (100 E2 mg per kg of diet) and a high-dose (150 E2 mg per kg of diet) experimental E2 treatment were fed to juvenile green sunfish from 30 to 90 days post-hatch. Fish were subsequently evaluated for any treatment effect …

Climate Change Alters Aging Patterns Of Reservoir Aquatic Habitats, Leandro E. Miranda, Nicky M. Faucheux

United States Geological Survey: Staff Publications

Two slow-moving developments are threatening reservoir aquatic habitats globally: aging and climate change. These events are projected to transform reservoir aquatic habitats in various and often unpredictable ways. Aging affects in-lake habitats directly, whereas climate change affects both in-lake and off-lake conditions. Climate change is expected to accelerate and, in some instances, possibly decelerate aging. Aging can be indexed as functional age, an index that signals the position of a reservoir along its lifespan relying on inlake descriptors of aquatic habitat. Using existing habitat datasets and climate projections, we developed semi-quantitative predictions about the effect of climate change on reservoir …

Sediment Source Fingerprinting As An Aid To Large-Scale Landscape Conservation And Restoration: A Review For The Mississippi River Basin, Zhen Xu, Patrick Belmont, Janice Brahney, Allen C. Gellis

United States Geological Survey: Staff Publications

Reliable quantitative information on sediment sources to rivers is critical to mitigate contamination and target conservation and restoration actions. However, for large-scale river basins, determination of the relative importance of sediment sources is complicated by spatiotemporal variability in erosional processes and sediment sources, heterogeneity in sediment transport and deposition, and a paucity of sediment monitoring data. Sediment source fingerprinting is an increasingly adopted field-based technique that identifies the nature and relative source contribution of sediment transported in waterways. Notably, sediment source fingerprinting provides information that is independent of other field, modeling, or remotely sensed techniques. However, the diversity in sampling, …

Seasonality Of Precipitation In The Southwestern United States During The Late Pleistocene Inferred From Stable Isotopes In Herbivore Tooth Enamel, Matthew J. Kohn, Kathleen B. Springer, Jeffrey S. Pigati, Linda M. Reynard, Amanda E. Drewicz, Justin Crevier, Eric Scott

United States Geological Survey: Staff Publications

The late Pleistocene was a climatically dynamic period, with abrupt shifts between cool-wet and warmdry conditions. Increased effective precipitation supported large pluvial lakes and long-lived spring ecosystems in valleys and basins throughout the western and southwestern U.S., but the source and seasonality of the increased precipitation are debated. Increases in the proportions of C₄/(C₄+ C₃) grasses in the diets of large grazers have been ascribed both to increases in summer precipitation and lower atmospheric CO₂ levels. Here we present stable carbon and oxygen isotope data from tooth enamel of late Pleistocene herbivores recovered …

Collateral Damage: Anticoagulant Rodenticides Pose Threats To California Condors, Garth Herring, Collin A. Eagles-Smith, Rachel Wolstenholme, Alacia Welch, Chris West, Barnett A. Rattner

United States Geological Survey: Staff Publications

Anticoagulant rodenticides (ARs) are widespread environmental contaminants that pose risks to scavenging birds because they routinely occur within their prey and can cause secondary poisoning. However, little is known about AR exposure in one of the rarest avian scavengers in the world, the California condor (Gymnogyps californianus). We assessed AR exposure in California condors and surrogate turkey vultures (Cathartes aura) to gauge potential hazard to a proposed future condor flock by determining how application rate and environmental factors influence exposure. Additionally, we examined whether ARs might be correlated with prolonged blood clotting time and potential mortality …

Limited Rigor In Studies Of Raptor Mortality And Mitigation At Wind Power Facilities, Tara J. Conkling, Christopher J.W. Mcclure, Sandra Cuadros, Scott R. Loss, Todd E. Katzner

United States Geological Survey: Staff Publications

Wind power is an expanding source of renewable energy. However, there are ecological challenges related to wind energy generation, including collisions of wildlife with turbines. Lack of rigor, and variation in study design, together limit efforts to understand the broad-scale effects of wind power infrastructure on wildlife populations. It is not clear, however, whether these types of limitations apply to groups of birds such as raptors that are particularly vulnerable to negative effects of wind energy. We reviewed 672 peer-reviewed publications, unpublished reports, and citations from 321 wind facilities in 12 countries to evaluate methods used to monitor and mitigate …

Investigation Of Dead Ocean Quahogs (Arctica Islandica) Shells On The Mid-Atlantic Bight Continental Shelf, Alyssa Leclaire

Master's Theses

Ocean quahogs, Arctica islandica, are a long-lived, widely dispersed, biomass dominate in the Mid-Atlantic; therefore, quahog shells are valuable resources for studying climate change over time. Recently, dead ocean quahog shells were discovered south and inshore of the present biogeographic range of this animal. The presence of ocean quahog shells outside the current range is presumably a consequence of past regressions and transgressions of the Cold Pool, the bottom-trapped, cool body of water that allows boreal animals to live at lower latitudes. Dead ocean quahog shells were collected offshore of the DelMarVa Peninsula then radiocarbon-dated, evaluated for taphonomic condition, …

Spatial Stream Modeling Of Louisiana Waterthrush (Parkesia Motacilla) Foraging Substrate And Aquatic Prey In A Watershed Undergoing Shale Gas Development, Mack W. Frantz, Petra B. Wood, Steven C. Latta

United States Geological Survey: Staff Publications

We demonstrate the use of spatial stream network models (SSNMs) to explore relationships between a semiaquatic bioindicator songbird, Louisiana Waterthrush (Parkesia motacilla), and stream monitoring and benthic macroinvertebrate data in an area undergoing shale gas development. SSNMs allowed us to account for spatial autocorrelation inherent to these environmental data types and stream properties that traditional modeling approaches cannot capture to elucidate factors that affect waterthrush foraging locations. We monitored waterthrush along 58.1 km of 1st- and 2nd-order headwater stream tributaries (n = 14) in northwestern West Virginia over a two year period (2013–2014), sampled benthic macroinvertebrates in waterthrush …

Science Of The Total Environment, Benjamin Linhoff

United States Geological Survey: Staff Publications

Recently, the subsoils of ephemeral stream(arroyos) floodplains in the northern Chihuahuan Desert were discovered to contain large naturally occurring NO₃⁻ reservoirs (floodplain:~38,000 kg NO₃-N/ha; background:~60 kg NO₃-N/ha). These reservoirs may be mobilized through land use change or natural stream channel migration which makes differentiating between anthropogenic and natural groundwaterNO₃⁻sources challenging. In this study, the fate and sources of NO₃⁻ were investigated in an area with multiple NO₃⁻ sources such as accidental sewer line releases and sewage lagoons aswell as natural reservoirs of subsoil NO₃⁻. …

Droughtscape, Summer 2022, National Drought Mitigation Center

DroughtScape: Quarterly Newsletter of the National Drought Mitigation Center (2007–2023)

DroughtScape, Summer 2022

Population Genetics Reveals Bidirectional Fish Movement Across The Continental Divide Via An Interbasin Water Transfer, Audrey C. Harris, Sara J. Oyler-Mccance, Jennifer A. Fike, Matthew P. Fairchild, Christopher M. Kennedy, Harry J. Crockett, Dana L. Winkelman, Yoichiro Kanno

United States Geological Survey: Staff Publications

Interbasin water transfers are becoming an increasingly common tool to satisfy municipal and agricultural water demand, but their impacts on movement and gene flow of aquatic organisms are poorly understood. The Grand Ditch is an interbasin water transfer that diverts water from tributaries of the upper Colorado River on the west side of the Continental Divide to the upper Cache la Poudre River on the east side of the Continental Divide. We used single nucleotide polymorphisms to characterize population genetic structure in cutthroat trout (Oncorhynchus clarkii) and determine if fish utilize the Grand Ditch as a movement corridor. …

Foraging Habitat Selection Of Shrubland Bird Community In Tropical Dry Forest, Anant Deshwal, Steven L. Stephenson, Pooja Panwar, Brett A. Degregorio, Ragupathy Kannan, John D. Willson

United States Geological Survey: Staff Publications

Habitat loss due to increasing anthropogenic disturbance is the major driver for bird population declines across the globe. Within the Eastern Ghats of India, shrubland bird communities are threatened by shrinking of suitable habitats due to increased anthropogenic disturbance and climate change. The development of an effective habitat management strategy is hampered by the absence of data for this bird community. To address this knowledge gap, we examined foraging sites for 14 shrubland bird species, including three declining species, in three study areas representing the shrubland type of forest community in the Eastern Ghats. We recorded microhabitat features within an …

Speciation With Gene Flow In A Narrow Endemic West Virginia Cave Salamander (Gyrinophilus Subterraneus), Evan H. Campbell Grant, Kevin P. Mulder, Adrianne B. Brand, Douglas B. Chambers, Addison H. Wynn, Grace Capshaw, Matthew L. Niemiller, John G. Phillips, Jeremy F. Jacobs, Shawn R. Kuchta, Rayna C. Bell

United States Geological Survey: Staff Publications

Due to their limited geographic distributions and specialized ecologies, cave species are often highly endemic and can be especially vulnerable to habitat degradation within and surrounding the cave systems they inhabit. We investigated the evolutionary history of the West Virginia Spring Salamander (Gyrinophilus subterraneus), estimated the population trend from historic and current survey data, and assessed the current potential for water quality threats to the cave habitat. Our genomic data (mtDNA sequence and ddRADseq-derived SNPs) reveal two, distinct evolutionary lineages within General Davis Cave corresponding to G. subterraneus and its widely distributed sister species, Gyrinophilus porphyriticus, that …

Toxicological Responses To Sublethal Anticoagulant Rodenticide Exposure In Free‑Flying Hawks, Nimish B. Vyas, Barnett A. Rattner, J. Michael Lockhart, Craig S. Hulse, Clifford P. Rice, Frank Kuncir, Kevin Kritz

United States Geological Survey: Staff Publications

An important component of assessing the hazards of anticoagulant rodenticides to non-target wildlife is observations in exposed free-ranging individuals. The objective of this study was to determine whether environmentally realistic, sublethal first-generation anticoagulant rodenticide (FGAR) exposures via prey can result in direct or indirect adverse effects to free-flying raptors. We offered black-tailed prairie dogs (Cynomys ludovicianus) that had fed on Rozol® Prairie Dog Bait (Rozol, 0.005% active ingredient chlorophacinone, CPN) to six wild-caught red-tailed hawks (RTHA, Buteo jamaicensis), and also offered black-tailed prairie dogs that were not exposed to Rozol to another two wild-caught RTHAs for 7 …

Within‑Marsh And Landscape Features Structure Ribbed Mussel Distribution In Georgia, Usa, Marshes, William K. Annis, Elizabeth A. Hunter, John M. Carroll

United States Geological Survey: Staff Publications

Ribbed mussels, Geukensia demissa, are marsh fauna that are used in coastal management and restoration due to the ecosystem services they provide. Ribbed mussel restoration efforts may be improved with a greater understanding of the environmental drivers of ribbed mussel distribution at multiple spatial scales to predict areas where restoration could be successful. This study sought to estimate the effects of within-marsh (4 m) and landscape (500 m) factors on ribbed mussel distribution. Ribbed mussel densities were surveyed at 11 sites along the coast of Georgia, USA, and overlaid with spatial data for within-marsh factors (elevation, distance to marsh …

Influences Of Seasonality And Habitat Quality On Great Lakes Coastal Wetland Fish Community Composition And Diets, Sara N. Diller, Anna M. Harrison, Kurt P. Kowalski, Valerie J. Brady, Jan J. H. Ciborowski, Matthew J. Cooper, Joshua D. Dumke, Joseph P. Gathman, Carl R. Ruetz Iii, Donald G. Uzarski, Douglas A. Wilcox, Jeffrey S. Schaeffer

United States Geological Survey: Staff Publications

Great Lakes coastal wetlands (GLCW) have been severely degraded by anthropogenic activity over the last several decades despite their critical role in fish production. Many Great Lakes fish species use coastal wetland habitats for spawning, feeding, shelter, and nurseries throughout the year. The goal of our study was to compare GLCW fish community composition in the spring, summer, and fall months and investigate how water quality relates to fish diversity, the presence of functional groups, and juvenile fish diets. We summarized fish data collected from GLCW across the basin and used the coastal wetland monitoring program’s water quality-land use indicator …

A Critical Review Of Bioaccumulation And Biotransformation Of Organic Chemicals In Birds, Dave T. F. Kuo, Barnett A. Rattner, Sarah C. Marteinson, Robert Letcher, Kim J. Fernie, Gabriele Treu, Markus Deutsch, Mark S. Johnson, Sandrine Deglin, Michelle Embry

United States Geological Survey: Staff Publications

A literature review of bioaccumulation and biotransformation of organic chemicals in birds was undertaken, aiming to support scoping and prioritization of future research. The objectives were to characterize available bioaccumulation/ biotransformation data, identify knowledge gaps, determine how extant data can be used, and explore the strategy and steps forward. An intermediate approach balanced between expediency and rigor was taken given the vastness of the literature. Following a critical review of >500 peer-reviewed studies, >25,000 data entries and 2 million information bytes were compiled on >700 organic compounds for ~ 320 wild species and 60 domestic breeds of birds. These data …

Nutrient Restoration Of A Large, Impounded, Ultra-Oligotrophic Western River To Recover Declining Native Fishes, Ryan S. Hardy, Tyler J. Ross, Kevin N. Mcdonnell, Michael C. Quist, Charlie Holderman, Bryan S. Stevens

United States Geological Survey: Staff Publications

Declines in many fish populations in large, western rivers have been primarily attributed to the anthropogenic reduction of nutrient inputs and subsequent impacts to the food web. The largest known river fertilization program was implemented starting in 2005 on the Kootenai River in northern Idaho to restore resident fisheries. Annual electrofishing surveys were conducted at multiple sites in Idaho and Montana before and during nutrient addition to evaluate assemblage and population-level responses. Although few responses in fish assemblage structure were observed, the addition of liquid ammonium polyphosphate fertilizer (3 μg/L) to the Kootenai River increased fish abundance and biomass over …

Comparing Composite Severe Weather Indices Of Thunderstorm Activity On Sea-Breeze And Non-Sea-Breeze Days In The Mobile, Alabama Area, Elizabeth Seiler

Poster Presentations

Poster for the Honors Thesis project.

Sea breezes (SB) occur frequently from May through October along the U.S. Gulf of Mexico Coast. One reason why SBs interest forecasters is their ability to spawn summertime thunderstorms, which can cause flash flooding, lightning, and hail. However, forecasting the exact timing and location of SB-driven convection can be challenging. This thesis will focus on Mobile and Baldwin Counties in southwest Alabama, which experience two types of phenomena – SBs along the Gulf of Mexico Coast and bay breezes on either side of Mobile Bay. Over the past years, multiple undergraduate students have analyzed …

Comparing Composite Severe Weather Indices Of Thunderstorm Activity On Sea-Breeze And Non-Sea-Breeze Days In The Mobile, Alabama Area, Elizabeth Seiler

Honors Theses

Sea breezes (SB) occur frequently from May through October along the U.S. Gulf of Mexico Coast. One reason why SBs interest forecasters is their ability to spawn summertime thunderstorms, which can cause flash flooding, lightning, and hail. However, forecasting the exact timing and location of SB-driven convection can be challenging. This thesis will focus on Mobile and Baldwin Counties in southwest Alabama, which experience two types of phenomena – SBs along the Gulf of Mexico Coast and bay breezes on either side of Mobile Bay. Over the past years, multiple undergraduate students have analyzed Mobile, Alabama radar imagery of past …

The Impact Of Shoreline Stabilization On Sediment Supply And Ecogeomorphology: Assessing Marsh Response To Altered Wave Energies, Lake Borgne, Louisiana, Jessica J. Villers

LSU New Orleans Theses and Dissertations

Shoreline protection structures are a common response to lateral erosion in marshes, but limited studies exist on how they influence sediment supply and the resulting ecogeomorphology. This study investigates the impacts of marine mattresses, geotextile cages filled with riprap, in Lake Borgne, LA. A field study was conducted during cold front passages at a site with marine mattresses and a proximal control site without, collecting measurements of water depth, wave energy, lateral erosion, sediment accumulation, vegetation, and sediment characteristics. Wetlands with marine mattresses attenuated waves more than their natural marsh edge counterparts, which have limited conditions favorable for sedimentation. However, …

The Impact Of Sea-Level Rise In Numerically Modeled Landfalling Hurricanes: Katrina And The Gulf Coast., Serenity Nadirah Mercuri

Electronic Theses and Dissertations

With climate change, landfalling hurricanes become an increasing threat to coastal regions. However, the interactions between the coastal landscape and landfalling hurricanes are often overlooked when addressing sea-level rise outside of inundation and independent of sea surface temperature. This study analyzed the potential impacts regarding structure and intensity as a result of sea-level rise in the Gulf of Mexico using the WRF-ARW numerical model coupled with a 1D ocean model. Analysis showed that 10 m windspeed from landfall forward was higher in modified coastlines, and minimum sea-level pressure post-landfall was consistently lower for modified runs where storms maintain a higher …

Air, Land, And Water Variables Associated With The First Appearance And Current Spatial Distribution Of Toxic Prymnesium Parvum Blooms In Reservoirs Of The Southern Great Plains, Usa, Shisbeth Tábora-Sarmiento, Reynaldo Patiño, Carlos Portillo-Quintero, Cade Coldren

United States Geological Survey: Staff Publications

This study examined the association of air, land, and water variables with the first historical occurrence and current distribution of toxic Prymnesium parvum blooms in reservoirs of the Brazos River and Colorado River, Texas (USA). One impacted and one reference reservoir were selected per basin. Land cover and use variables were estimated for the whole watershed (WW) and a 0.5-km zone on either side of streams (near field, NF). Variables were expressed in annual values. Principal component and trend analyses were used to determine (1) differences in environmental conditions before and after the 2001 onset of toxic blooms in impacted …

Evaluating Alternative Ebullition Models For Predicting Peatland Methane Emission And Its Pathways Via Data–Model Fusion, Shuang Ma, Lifen Jiang, Rachel M. Wilson, Jeff P. Chanton, Scott Bridgham, Shuli Niu, Colleen M. Iversen, Avni Malhotra, Jiang Jiang, Xingjie Lu, Yuanyuan Huang, Jason Keller, Xiaofeng Xu, Daniel M. Ricciuto, Paul J. Hanson, Yiqi Luo

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Understanding the dynamics of peatland methane (CH4) emissions and quantifying sources of uncertainty in estimating peatland CH4 emissions are critical for mitigating climate change. The relative contributions of CH4 emission pathways through ebullition, plant-mediated transport, and diffusion, together with their different transport rates and vulnerability to oxidation, determine the quantity of CH4 to be oxidized before leaving the soil. Notwithstanding their importance, the relative contributions of the emission pathways are highly uncertain. In particular, the ebullition process is more uncertain and can lead to large uncertainties in modeled CH4 emissions. To improve model simulations of CH4 emission and its pathways, …

Spatial And Temporal Distribution And Habitat Selection Of Native Yellowstone Cutthroat Trout And Nonnative Utah Chub, Darcy K. Mccarrick, Jeffrey C. Dillon, Brett High, Michael C. Quist

United States Geological Survey: Staff Publications

Henrys Lake, Idaho, is a renowned trophy trout fishery that faces an uncertain future following the establishment of Utah Chub (UTC) Gila atraria. Utah Chub were first documented in the lake in 1993 and have become abundant over the past two decades. Little is known about the ecology of UTC, but they typically have negative effects on salmonids in systems where they have been introduced. We sought to fill knowledge gaps in UTC ecology and provide insight on potential interactions with Yellowstone Cutthroat Trout (YCT) Oncorhynchus clarkii bouvieri. Ninety-four YCT and 95 UTC were radio-tagged in spring 2019 …

Ignoring Species Availability Biases Occupancy Estimates In Single-Scale Occupancy Models, Graziella V. Direnzo, David A. W. Miller, Evan H. C. Grant

United States Geological Survey: Staff Publications

1. Most applications of single-scale occupancy models do not differentiate between availability and detectability, even though species availability is rarely equal to one. Species availability can be estimated using multi-scale occupancy models; however, for the practical application of multi-scale occupancy models, it can be unclear what a robust sampling design looks like and what the statistical properties of the multi-scale and single-scale occupancy models are when availability is less than one.
2. Using simulations, we explore the following common questions asked by ecologists during the design phase of a field study: (Q1) what is a robust sampling design for the multi-scale occupancy …