Water Resource Management Commons^™

Metabolic Capabilities Of Sulfur Oxidizing Bacteria And Their Role In Water Pollution, M. I. H. Aleem

KWRRI Research Reports

This report describes investigations into the physiology of microorganisms that are commonly involved in the oxidation of inorganic sulfur compounds. The metabolic activities of these bacteria play a potent role in several fields of economic importance such as strip mining operations, water pollution, corrosion, metallurgy, petroleum technology and soil fertility processes.

The oxidation of inorganic sulfur compounds was studied in the chemolithotrophs Thiobacillus denitrificans, Thiobacillus A₂, Thiobacillus neapolitanus, and a photolithotroph Rhodopseudomonas palustris. Cell suspensions from all of the thiobacilli catalyzed completely the oxidation of sulfide, thiosulfate, and sulfite to sulfate. The oxidation of thiosulfate in R. palustris …

Powers Of The State Of Kentucky In Implementing An Effluent Tax As A Part Of An Interstate Ohio River Basin Water Pollution Control Program, Anita L. Morse, Edward Zeigler

KWRRI Research Reports

This report is intended to set forth some of the problems and solutions involved in financing and regulating water quality control. The purpose is to record some of the major problems confronting those who legislate water quality, those who espouse technological answers, and those who see the problem in terms of economic solutions. The limits placed by political and institutional constraints on solutions to these problems are frequently not understandable.

Within this report are contained separate investigations: a study of federal-interstate relations and the interstate compact; a study of Kentucky's common law approach to water rights; a study of financing …

Analysis Of Unsteady Flow Toward Artesian Wells By Three-Dimensional Finite Elements, Yang H. Huang, Shen-Jyh Wu

KWRRI Research Reports

A three-dimensional finite element computer program was developed for analyzing unsteady flow toward artesian wells. The program is designed especially for determining the drawdown around an artesian well penetrating fully or partially a nonhomogeneous and anisotropic aquifer of irregular shape and cross section. It can also be used as a general program for aquifer simulation and evaluation. A major advantage of the program lies in the minimum amount of input data required. By assuming the top and bottom boundaries of the aquifer as two arbitrary planes, the aquifer will be divided into six- or eight-node elements, and their nodal coordinates …

Seasonal Changes In Water Quality And Primary Productivity In Doe Valley Lake, Edmond J. Bacon, Stuart E. Neff

KWRRI Research Reports

Primary productivity and water quality were studied in Doe Valley Lake, a 147-hectare impoundment on Doe Run, a spring-fed stream in Meade County, Kentucky, from 13 June 1969 to 31 July 1972. Doe Valley Lake is monomictic during most winter seasons, but it is dimictic during more severe winters because of its morphometry and location on the borderline climatic region for dimictic lakes (37° N latitude). Oxygen depletion in the hypolimnion is severe, and anaerobic conditions usually prevail by late June. A hypolimnetic areal deficit of 0.038 mg/cm²/day was calculated. Supersaturation of oxygen in the epilimnion was common, …

Development Of Prediction Relationships For Water Requirements With Irrigation Cooling, Billy J. Barfield, John N. Walker, F. A. Payne

KWRRI Research Reports

A model for predicting leaf temperatures during an off-on mist cycle is presented. The model uses a combination of energy budget and aerodynamic techniques. The model was tested for dry leaf temperature and evapotranspiration predictions using average hourly data from Arizona. The accuracy was good. It was also tested for dry leaf temperature predictions using two to three minute data with a widely varying net radiation. When reasonable values of stomatal resistance were used, the agreement was again good.

The model was tested for prediction of wet leaf temperature prediction with a ten minute on, fifty minute off mist irrigation …

Changes In The Economy And Ecology At Proposed Lake Sites In The Salt River Basin, Kentucky, During Early Construction Of The Dam For Taylorsville Lake, Louis A. Krumholz, Stuart E. Neff, Edmond J. Bacon Jr., John R. Baker, Daryl E. Jennings, Andrew C. Miller, Vincent H. Resh, David S. White

KWRRI Research Reports

This is an extension of the work reported in Project numbers B-005-KY, B-016-KY, and B-022-KY that extended from 1 July 1968 through 30 June 1972. Permanent collecting stations have been established at 67 sites throughout the Salt River, Beech Fork, and Chaplin River drainages. Turbidities increases quickly as flow and runoff increase, and subside quickly when the rain stops. Suspended solids range up to 1,700 mg/l in high turbidities and vary considerably as a result of local spates. Water chemistry generally reflects the limestone nature of the substrate and physico-chemical characteristics of a typically healthy limestone stream. Bottom organisms are …

Sensitivity Of Vertebrate Embryos To Heavy Metals As A Criterion Of Water Quality, Phase I, Wesley J. Birge, John J. Just, Albert G. Westerman, A. Duane Rose

KWRRI Research Reports

Avian, amphibian and fish embryos were given continuous treatment with inorganic mercury, methyl mercury, cadmium and lead, to determine the sensitivity of embryogenesis to metallic poisoning. All metals produced substantial degrees of lethality and/or gross anatomical anomalies at 10 ppb or less. Treatment with inorganic mercury at 10 ppb produced 100% kill of frog embryos. Chick and rainbow trout embryos suffered 10-20% lethality when exposed to 1 ppb of either inorganic or methyl mercury. Lead and cadmium at 1 ppb produced 24-32% lethality in chick embryos. No significant differences were observed in the embryopathic effects of inorganic or methyl mercury. …

Social Costs And Benefits Of Water Resources Construction, Rabel J. Burdge, K. Sue Johnson

KWRRI Research Reports

This report analyzes and describes the process of relocating individuals and families who must move due to reservoir construction in Kentucky utilizing data collected in previous research. These data come from four separate studies: a study of community attitudes toward reservoir construction (Johnson county where the Paintsville reservoir is to be constructed), interviews with people who are slated for relocation when the Taylorsville reservoir is constructed, and two sets of interviews with people who have already been relocated due to reservoir construction (Cave Run and Carr Fork).

Psychological, social, economic and material costs and benefits associated with forced relocation are …

A Detailed Investigation Of The Sociological, Economic, And Ecological Aspects Of Proposed Reservoir Sites In The Salt River Basin Of Kentucky, Stuart E. Neff, Louis A. Krumholz, John R. Baker, Daryl E. Jennings, Andrew C. Miller, Jerry S. Parsons, Vincent H. Resh, David S. White

KWRRI Research Reports

Samples of water, bottom fauna, and fishes were collected from 66 stations in the Salt River and one of its principal tributaries, the Beech Fork and its tributary, the Chaplin River, Kentucky. Precipitation ranged from 38.86 inches (1969) to 58.04 inches (1970), an increase of nearly 50 percent with marked fluctuations in discharge. Intensive comparisons of phosphates, sulfates, specific conductance, total alkalinity, total hardness, and turbidity showed the streams to be relatively clean and healthy. Nearly 300 different kinds of benthic organisms and other macroinvertebrates have been collected and identified from the basin. Detailed studies of caddisflies and stream drift …

Sociocultural Impact Of Reservoirs On Local Government Institutions, Philip Drucker, Jerry Eugene Clark, Lesker Dianne Smith

KWRRI Research Reports

This study of the probable sociocultural impact of a proposed reservoir in central Kentucky on the institutions of local governments of a community adjacent to the reservoir utilizes anthropological concepts of social values and cultural and social change as well as anthropological research techniques. Data on observed impact on the same institutions in communities adjacent to two recently completed Kentucky reservoirs permit inferences as to probable directions and extent of reservoir-related change. Specific aspects of impact considered include: effects of reduction of the county tax base due to Federal acquisition of lands, including necessity for increased severity of taxes and …

Minimum Cost Design Of Water Distribution Systems, Don J. Wood, C. O. Charles

KWRRI Research Reports

The objective of this study was to develop the analytical tools and procedures for minimum cost design of water distribution systems. Both analog and digital means of carrying out pressure and flow calculations were developed. As a result of this effort, digital programs for pressure and flow calculations in water distribution systems were written and have been widely distributed to practicing engineers. One procedure is based on a direct solution of the basic system equations using a linearization scheme and has several advantages over conventional techniques such as the Hardy Cross method. These include avoiding the need to initially balance …

Enzyme And Tissue Alterations In Fishes: A Measure Of Water Quality, David E. Hinton, M. W. Kendall, J. C. Koenig

KWRRI Research Reports

A variety of freshwater fishes were studied by light and electron microscopy, enzyme histochemical and biochemical methods, The objective was to determine normal structure and function in specific target organs and to compare these to altered states in aquatic pollution. The basic question, "can fish tissues and enzymes serve as indicators of water quality?," was asked. Microscopic alteration in gill was indicative of copper toxicity at an exposure of 20 parts per billion, Gross and light microscopic alterations were indicative of a single exposure of channel catfish to 15 parts per million of methyl mercuric chloride (CH₃HgCl). Microscopic …

Process Control Of Activated Sludge Treatment, Richard I. Kermode, Robert W. J. Brett, Kenneth A. Debelak, John J. Davis

KWRRI Research Reports

General feed forward controllers, conforming to standard control modes, have been derived for an activated sludge process. The analysis indicated that the appropriate controllers are proportional control with measurement of substrate flow rate, and derivative control with measurement of inlet substrate concentration, and manipulation of the rate of return sludge by both controllers.

The performance of these controllers was tested by computer simulation of five dynamic aerator models with and without sludge storage, and with two settling basin models. In all cases significant reduction of the maximum exit substrate concentration was achieved. Additional improvement resulted from the use of sludge …

Sensitivity Of Vertebrate Embryos To Heavy Metals As A Criterion Of Water Quality, Wesley J. Birge, John J. Just

KWRRI Research Reports

Embryonic and/or larval stages of the leopard frog (Rana pipiens), domestic fowl (Gallus domesticus) and the goldfish (Carassiua auratus) were treated with cadmium chloride, mercuric chloride, lead chloride, zinc chloride and sodium arsenite. The principal objectives were (1) to determine the sensitivity of vertebrate embryos to certain metals which are of consequence in water pollution, and (2) to ascertain the suitalility of vertebrate embryos as bioassay organisms for monitoring metallic pollutants within water resources. Vertebrate embryos are found to be highly sensitive to the toxic effects of all the metals studied. Concentrations of mercury as low as 10 ppb, with …

Social And Cultural Impact Of A Proposed Reservoir On A Rural Kentucky School District, Charles R. Smith, Philip Drucker

KWRRI Research Reports

This study utilizes anthropological concepts and research methods to study the educational system of a Central Kentucky school district with the goal of predicting the impact on it of a proposed multi-purpose (flood control, recreation, etc.) reservoir, and proposing options for forestalling dysfunctional aspects of that impact. The impact will result from the fact that although the county is now rurally oriented, the proposed reservoir will attract (has already begun to attract) urbanite residents from nearby Louisville, Kentucky's largest urban center, who can be expected to bring urban values concerning education, as well as other areas. To assess probable directions …

Characterization Of Water Movement Into And Through Soils During And Immediately After Rainstorms, C. T. Haan

KWRRI Research Reports

The movement of water into and through soils in the unsaturated state is basic to many water resources problems including rainfall-runoff models, ground water recharge, irrigation, drainage, evapotranspiration and the movement of pollutants in soils. This study was conducted in an effort to determine if the flow equation based on Darcy's Law and the continuity equation could be used to describe watershed infiltration and thus be incorporated into hydrologic models.

The results of the study indicate that even on apparently uniform soils there is a great deal of variability in soil water properties. Handling this variability plus the difficulty of …

Development And Testing Of A Double-Beam Absorption Spectrograph For The Simultaneous Determination Of Different Cations In Water, William H. Dennen

KWRRI Research Reports

Construction and testing of a double-beam absorption spectrographic analysis system using a d. c. arc multielement source has been conducted. An optical system design which brings analytical and reference beams together to illuminate the upper and lower portions of the spectrograph slit has been shown to be functional. However, ad. c. arc will not serve as a multielement source for the intended purposes because of excessive thermal broadening of emission lines.

A direct excitation d. c. arc method was reviewed which can be used for the rapid determination of some cations in water.

The Relation Between Soil Characteristics, Water Movement And Nitrate Contamination Of Ground Water, Grant W. Thomas, Matthew Mcmahon

KWRRI Research Reports

Soils from several areas in Kentucky were placed in columns and leached with Ca(NO₃)₂. Subsoils high in iron oxide were found to retard the leaching of nitrate very significantly. In other soils, the nitrate moved through as fast as or slightly faster than the water.

Field application of nitrogen to corn was most efficient when done in the spring or summer near the time that the corn takes it up. The one exception to this was a red soil, where fall application of nitrogen resulted in little loss due to the retarding effect mentioned in the …

Location Of Solution Channels And Sinkholes At Dam Sites And Backwater Areas By Seismic Methods: Part Ii, Vincent P. Drnevich, D. Raghu

KWRRI Research Reports

Four seismic field methods and a laboratory method are used to determine shear wave propagation velocities and shear moduli for two sites. The four seismic methods are: standard seismic refraction survey, down hole shooting refraction survey, transient Rayleigh wave survey, and crosshole shooting survey. A torsional resonant column apparatus was used for the laboratory tests. The cross hole shooting method gave the best results because direct measurements were made. Criteria for using this method are given. Methods which measure compression wave velocity give inconsistent results because the conversion to shear wave velocity is very sensitive to Poisson's ratio. Laboratory tests …

Location Of Solution Channels And Sinkholes At Dam Sites And Backwater Areas By Seismic Methods: Part I, Vincent P. Drnevich, S. R. Smith, E. P. Cleveland

KWRRI Research Reports

The basic concepts associated with the sledge hammer seismic refraction survey are reviewed and a modified version called down hole shooting is discussed. The latter method has distinct advantages for rock surface profiling. These include: calibration at the end points of the survey, measurement of vertical wave propagation velocities directly, and having a refracted wave ray path for almost the entire survey length.

The down hole shooting seismic refraction survey has been simulated with the digital computer. The method can handle any shaped rock surface profile and generates corresponding travel time curves for the forward and reverse profile surveys. This …

Hydraulic And Sediment Transport Studies In Relation To River Sediment Control And Solid Waste Pollution And Economic Use Of The By-Products, Bruce R. Moore, Michael R. Short

KWRRI Research Reports

The distribution of sediments and conditions of transport were studied in the Kentucky, Big Sandy and Ohio Rivers. The sand and coal were in transport at different flow velocities for the rivers and the deposition of these sediments was a direct function of the flow conditions at a particular locality.

The flow conditions of transport of the sediments were studied in flumes as were the hydraulic conditions in model dredge holes to determine the feasibility of trapping sediment. The conditions of scour and fill were also established and compared with known conditions in a dredge hole in the Ohio River. …

Impact Of A Proposed Reservoir On Local Land Values, Philip Drucker, Charles R. Smith, Allen C. Turner

KWRRI Research Reports

This project was designed to study the impact of private preconstruction land acquisition in a Central Kentucky area in which a flood-control dam and reservoir impoundment is planned. The research design was built around use of anthropological concepts and research techniques. By these means it was possible to analyze the perceived values of land to traditionalist residents, among which social and economic security concepts are paramount. Recent land purchases, locally believed to have been made chiefly by "outsiders," (urbanites from nearby Louisville), at prices out of line in terms of local agricultural worth, has been extremely unsettling contributing to the …

A Perspective On Economic Impact, L. Douglas James, Donald M. Soule, William O. Thompson, John L. Fulmer, John C. Redman, Robert C. Tussey, John M. Higgins, Claude M. Vaughan, David H. Rosenbaum, Billy R. Prebble, Charles O. Dowell, John E. Sirles, Michael B. Hargrove, Clyde T. Bates, Kenneth G. Holbrook, Dennis H. Bianchi, John P. Breaden, Kenneth R. Harman

KWRRI Research Reports

The institutions responsible for water resources management in the United States have originated as political responses to major social issues. Each agency institutionalized a procedure for structuring and comparing alternatives in the formulation of its total program. Each agency originally sought to promote effective resolution of its social issue (flood control, development of arid lands, soil erosion, etc.), but more recent efforts have sought better coordination among agency practices through a common procedure largely derived from economic theory. Any procedure, however, varies in application with the interpretation and judgment of individual planners. Today, public pressures have brought political directives requiring …

Carbon And Nitrogen As Regulators Of Algal Growth In Treated Sewage, Edward G. Foree, Charles Reece Scroggin

KWRRI Research Reports

Continuous flow algal cultures were grown under three different growth conditions using secondary sewage treatment plant effluent as the growth medium. The only variable within each run was the hydraulic residence time. The concentrations of growth regulating nutrients were varied between the runs so comparisons of the algal mass, composition, nutrient uptake, and genera could be made. The importance of CO₂ availability for algal growth was also studied. A kinetic theory which based algal growth on cellular nutrient concentration was verified. The second phase of the study was a batch culture study in which the same growth medium was …

Determination Of Trace Elements In Water Utilizaing Atomic Absorption Spectroscopy Measurement, Gary D. Christian, J. Preston Miles Iii, Ronald Sensmeier, Melvin Sensmeier, Loraine Craig, Lynn Phibrick, Sudarshan Lal

KWRRI Research Reports

A variety of flameless atomic absorption methods have been investigated for the possible determination of trace elements in water. The general applicability of each technique was determined and where applicable, procedures have been developed for the determination of trace elements in natural waters.

Metals were preconcentrated by electrodeposition on a wire. The metals were then atomized for atomic absorption measurement by resistive heating of the wire. As little as 0.01 part per million (ppm) of mercury could be determined in this way.

A mercury cold vapor cell was constructed in which the sample is treated with a reducing agent to …

Degradation Of Riparian Leaves And The Recycling Of Nutrients In A Stream Ecosystem, Louis A. Krumholz, Roger G. Lambert, Charles R. Liston, Harry H. Woodward

KWRRI Research Reports

Leaves collected at 4 stations in the upper 5 km of Doe Run, Meade County, Kentucky, indicated an annual accumulation within the stream of 354 g/m²/year (17,700 kg). Leaves of sycamore (23.6%), red oak (21.7%), sugar maple (9.7%), beech (9.6%), white oak (7.1%), and hickory (6.0%) trees were most abundant, and leaves from 14 other kinds made up the remaining 22.3%. About a third of the annual leaf fall occurred during the last half of October and about two-thirds in the last 3 months of the year.

Calorific equivalents for different kinds of leaves ranged from 3,789 cal/g …

Factors Regulating The Growth Of Algae In Continuous Culture In Diluted Secondary Sewage Treatment Plant Effluent And Subsequent Biodegradability, Edward G. Foree, Caroline P. Wade

KWRRI Research Reports

Heterogeneous algal cultures were grown in laboratory continuous culture in continuous flow, completely mixed chemostats in secondary sewage treatment plant effluent diluted to give an ammonia nitrogen concentration of 10 mg/1. Variables were lighting, pH, carbon dioxide availability, and hydraulic residence time.

Optimum growth occurred under pH 7.0, excess CO₂, and continuous lighting conditions. The availability of artificially supplied excess CO₂ greatly increased the mass (standing crop) at steady-state over that produced under otherwise identical conditions for all residence times studied. For the case of excess CO₂ availability, the nitrogen concentration in the algal cells regulated …

Metabolic Role Of Sulfates And Sulfides Producing Bacteria In Pollution Of Waters, M. I. H. Aleem

KWRRI Research Reports

Cytochrome transport particles from Nitrobacter catalyzed nitrite, ascorbate as well as NADH oxidation with concomitant phosphate esterification yielding P/0 ratios of 1.0, 0.6 and 2.0 respectively. Phosphorylation coupled to nitrite oxidation was not effected by rotenone, amytal or antimycin while 50 and 70% inhibition of the NADH-linked phosphorylation was observed in the presence of HOQNO and rotenone respectively. Cell-free extracts from Nitrobacter also catalyzed an energy-dependent reduction of NAD⁺ by nitrite. The reduction of cytochrome c by NŌ₂ was energy-dependent which involved the reversal of electrons from cytochrome a₁. The subsequent energy-linked reduction of the flavoproteins …

Capillary-Diffusion And Self-Diffusion Of Liquid Water In Unsaturated Soils, Ronald E. Phillips, V. L. Quisenberry Jr.

KWRRI Research Reports

Capillary-diffusion coefficients were measured by use of inflow and outflow methods. With both methods the capillary-diffusion coefficients decreased very rapidly with decreasing water content. The lighter textured soils were found to have the higher diffusion coefficients over the entire moisture content range studied, 0 to 1 bar tension.

Self-diffusion coefficients were measured over a moisture content range from air dryness to saturation using ³H as a tracer of water. Each of the soils gave the same diffusion characteristics when the self-diffusion coefficients were expressed as a function of either water content or average number of water layers on the …

A Preliminary Ecological Study Of Areas To Be Impounded In The Salt River Basin Of Kentucky, Louis A. Krumholz, Stuart E. Neff, Edmond J. Bacon, Jerry S. Parsons, John D. Woodling

KWRRI Research Reports

This report includes work that is an extension of Project No. B-005-KY as reported in Research Report No. 43 of the University of Kentucky Water Resources Institute. That project was initiated in April 1968 as Project No. A-019-KY with principal emphasis on physical, chemical, and biological characteristics of the main stem of the Salt River upstream from the proposed damsite for Taylorsville Lake, an impoundment of about 3, 600 acres at seasonal pool. The report includes descriptions of an additional 13 stations along the stream, bringing to 38 the number of permanent collecting sites.

Values for dissolved oxygen ranged from …