Natural Resource Economics Commons^™

When Words Flow Like Water: How The Enbridge Line 3 Pipeline Environmental Impact Statement Failed To Prevent Hydrogeologic Harm In Minnesota, Carly Gutzmann

Journal of Earth and Life Science

For the aquifers of Minnesota, the environmental impact statement (EIS) was a promise of protection that never left the page. An environmental impact statement is meant to be an aid in the decision making process in order to ensure that projects consider potential environmental harms that may occur. However, they are often used instead as another regulatory box to check, rather than as active considerations when planning. As such, a project plan can be flawed from the start—if project developers only consider environmental impacts after they have already put considerable time, effort, and funding into their project as-is, they may …

Powering The Machine, Draining The Planet: Whether U.S. Environmental Law Is Equipped To Regulate The Energy And Water Demands Of Ai Data Centers, Michael Marcu

Journal of Earth and Life Science

Artificial intelligence (AI) data centers have become one of the United States' fastest-growing and least-regulated sources of environmental stress. In 2024 alone, U.S. data centers consumed 183 terawatt-hours (TWh) of electricity more than the entire nation of Pakistan and consumed an estimated 17 billion gallons of water (IEA, 2025; Berkeley Lab, 2024). By 2030, electricity demand from these facilities is projected to reach 426 TWh, a 133% increase in six years (Pew Research Center, 2025). This paper examines whether the existing U.S. environmental regulatory framework put by the National Environmental Policy Act (NEPA), the Clean Water Act (CWA), and the …

Who Owns The Wind: The Absence Of Community Wind Farms In California, Sky Berry-Weiss

Master's Projects and Capstones

Community ownership structures for wind farms have been around for decades, particularly in European countries, due to high socioeconomic benefits. Given these significant benefits, one might expect community wind to thrive in the United States—especially in a state like California, which prides itself on progressive climate policy and renewable energy leadership. Yet utility-scale community wind remains largely absent from research on California’s energy system, raising questions about its existence in the state. To pinpoint how many utility-scale community owned wind farms are in California, this study surveys every operational wind turbine in the state. After classifying each wind farm by …

Recent U.S. Government Policy Literature On Critical And Strategic Minerals, Bert Chapman

Libraries Faculty and Staff Scholarship and Research

Critical and strategic minerals have become increasingly important in U.S. government civilian and military policymaking in recent years. This is demonstrated by the heavy use of such minerals in many critical civilian and military infrastructures. This work will discuss how this subject has been addressed in laws, presidential documents, and works by government agencies along with congressional oversight committees and support agencies. It will stress how the United States is heavily dependent on strategic minerals from adversarial foreign countries such as China and will examine U.S. efforts to increase its ability to produce such materials in the United States by …

The Growth Of Ethanol In Brazil: Sugarcane, Corn, And A Few Other Things, Fabio Mattos

Cornhusker Economics

Although we can expect more ethanol produced from corn in the coming years, it is unclear whether it will keep expanding at the same pace as in recent years or how much it will contribute to total ethanol production.

One point to keep an eye on is the typical dynamics of markets. If supply and demand conditions and price movements make corn-ethanol more profitable, ethanol plants in Brazil will keep increasing their production. If it makes more economic sense to produce corn-ethanol (as it happened recently), they will use more corn to produce ethanol.

Supply and demand conditions for ethanol, …

Reducing Food Scarcity: The Benefits Of Urban Farming, S.A. Claudell, Emilio Mejia

Journal of Nonprofit Innovation

Urban farming can enhance the lives of communities and help reduce food scarcity. This paper presents a conceptual prototype of an efficient urban farming community that can be scaled for a single apartment building or an entire community across all global geoeconomics regions, including densely populated cities and rural, developing towns and communities. When deployed in coordination with smart crop choices, local farm support, and efficient transportation then the result isn’t just sustainability, but also increasing fresh produce accessibility, optimizing nutritional value, eliminating the use of ‘forever chemicals’, reducing transportation costs, and fostering global environmental benefits.

Imagine Doris, who is …

Food-Water-Energy Nexus In The Perspective Of Green Revolution, Green Energy, Legal And Institutional Framework: A Killian Based Adjusted Bootstrap Approach, Zia Ur Rahman

CBER Conference

Food and water energy is crucial for human well-being, sustainable development, and poverty reduction. The growing global demand driven by population growth, economic development, urbanization, changing diets, technological advancements, and climate change projections indicates a significant increase in the need for these resources. Understanding the intricate interdependencies between food, water, and energy is essential for effectively addressing these challenges and fostering a prosperous and sustainable future. Therefore, this study incorporated statistical data collected from the Pakistan Economic Survey and the World Governance Indicator from 1990 to 2022 to elucidate the complex connection between food, water, and energy.

Does Drought Stress Eliminate The Benefit Of Elevated Co2,/Sub> On Soybean Yield? Using An Improved Model To Link Crop And Soil Water Relations, Wenguang Sun, David Fleisher, Dennis Timlin, Chittaranjan Ray, Zhuangji Wang, Sahila Beegum, Vangimalla Reddy

Daugherty Water for Food Global Institute: Faculty Publications

Crop simulation models are indispensable tools that facilitate studies to assess climate impacts and adaptation responses, but have not been adequately tested in terms of accurately predicting crop growth and water stress responses to high carbon dioxide concentrations [CO₂]. The soybean model, GLYCIM, previously modified with a coupled leaf-level gas exchange – energy balance model, was integrated with a two-dimensional convectivediffusive root growth module which linked soil and leaf water potentials with the regulation of stomatal conductance. We evaluated the accuracy of this modified GLYCIM using experimental data from a Free-Air CO₂ Enrichment (FACE) site, SoyFACE, and …

Dual Activation Of Peroxymonosulfate Using Mnfe2O4/G‑C3N4 And Visible Light For The Efficient Degradation Of Steroid Hormones: Performance, Mechanisms, And Environmental Impacts, Kitipong Poomipuen, Chainarong Sakulthaew, Chanat Chokejaroenrat, Athaphon Angkaew, Kanidrawee Techauay, Thapanee Poompoung, Kanokwan Teingtham, Piyaporn Phansak, Piyangkun Lueangjaroenkit, Daniel D. Snow

Daugherty Water for Food Global Institute: Faculty Publications

Single activation of peroxymonosulfate (PMS) in a homogeneous system is sometimes insufficient for producing reactive oxygen species (ROS) for water treatment applications. In this work, manganese spinel ferrite and graphitic carbon nitride (MnFe₂O₄/g-C₃N₄; MnF) were successfully used as an activator for PMS under visible light irradiation to remove the four-mostdetected- hormone-contaminated water under different environmental conditions. The incorporation of g-C₃N₄ in the nanocomposites led to material enhancements, including increased crystallinity, reduced particle agglomeration, amplified magnetism, improved recyclability, and increased active surface area, thereby facilitating the PMS activation and electron …

Water-Energy-Food Linkages In Community Smallholder Irrigation Schemes: Center Pivot Irrigation In Rwanda, Ankit Chandra, Derek M. Heeren, Lameck O. Odhiambo, N. Brozovic

Daugherty Water for Food Global Institute: Faculty Publications

Water, energy, and food are linked in intricate ways in irrigated agriculture and understanding the interplay of these components is crucial for sustainable and profitable crop production, particularly in smallholder setting such as in sub-Saharan Africa. This study evaluates water-energy-food linkages, engineering and economic performance, irrigation decision making, and challenges faced around water management in a community-based mechanized irrigation scheme in Rwanda. The research is the first to analyze such as scheme, which uses technology typically used by large farmers in a smallholder setting. The study investigates the variation in water requirements and the relationship and impacts of this variability …

The Association Between Drought Exposure And Respiratory-Related Mortality In The United States From 2000 To 2018, Yeongjin Gwon, Yuanyuan Ji, Jesse E. Bell, Azar M. Abadi, Jesse D. Berman, Austin Rau, Ronald D. Leeper, Jared Rennie

Daugherty Water for Food Global Institute: Faculty Publications

Climate change has brought increasing attention to the assessment of health risks associated with climate and extreme events. Drought is a complex climate phenomenon that has been increasing in frequency and severity both locally and globally due to climate change. However, the health risks of drought are often overlooked, especially in places such as the United States, as the pathways to health impacts are complex and indirect. This study aims to conduct a comprehensive assessment of the effects of monthly drought exposure on respiratory mortality for NOAA climate regions in the United States from 2000 to 2018. A two-stage model …

Downstream Hydrochemistry And Irrigation Water Quality Of The Syr Darya, Aral Sea Basin, South Kazakhstan, Bagdat Satybaldiyev, Baimurat Ismailov, Nurbek Nurpeisov, Kairat Kenges, Daniel D. Snow, Arindam Malakar, Omirzhan Taukebayev, Bolat Uralbekov

Daugherty Water for Food Global Institute: Faculty Publications

New hydrochemical measurements from the Syr Darya provide insights into factors affecting the composition and quality of a major freshwater source replenishing the Aral Sea. This river is heavily used for power and irrigation and crosses territories of four Central Asia republics. It is intensely managed, draining several major tributaries, many reservoirs, and numerous irrigation distribution systems and canals. Analysis of seasonal changes in dissolved ion concentrations using geochemical diagrams, elemental ratios, statistical correlation, and equilibrium modeling allowed the characterization of mineral formation processes that control the dissolved chemical composition. Measured water hydrochemistry and composition type differs substantially from previous …

Templates To Transition: Making Solar-Generated Electricity Accessible For Lagos Residents, Adeola O. Adebajo

Senior Theses

According to the EPA, direct energy production results in at least 25% of the world’s emissions. This refers to emissions produced by burning coal, natural gas, and oil for electricity and heat. With the increasing effects of climate change, sustainable energy is becoming increasingly important as the world shifts away from fossil fuels. However, implementation of renewable energy sources is not as fast or as widespread as necessary. This paper aims to identify feasible solutions to the problem of renewable energy affordability in Nigeria especially in growing megacities like Lagos to ensure long lasting and climatefriendly energy sources. Two major …

Pan-Arctic Soil Moisture Control On Tundra Carbon Sequestration And Plant Productivity, Donatella Zona, Peter M. Lafleur, Koen Hufkens, Beniamino Gioli, Barbara Bailey, George Burba, Eugénie S. Euskirchen, Jennifer D. Watts, Kyle A. Arndt, Mary Farina, John S. Kimball, Martin Heimann, Mathias Göckede, Martijn Pallandt, Torben R. Christensen, Mikhail Mastepanov, Efrén López-Blanco, Albertus J. Dolman, Roisin Commane, Charles E. Miller, Josh Hashemi, Lars Kutzbach, David Holl, Julia Boike, Christian Wille, Torsten Sachs, Aram Kalhori, Elyn R. Humphreys, Oliver Sonnentag, Gesa Meyer, Gabriel H. Gosselin, Philip Marsh, Walter C. Oechel

Daugherty Water for Food Global Institute: Faculty Publications

Long-term atmospheric CO₂ concentration records have suggested a reduction in the positive effect of warming on high-latitude carbon uptake since the 1990s. A variety of mechanisms have been proposed to explain the reduced net carbon sink of northern ecosystems with increased air temperature, including water stress on vegetation and increased respiration over recent decades. However, the lack of consistent long-term carbon flux and in situ soil moisture data has severely limited our ability to identify the mechanisms responsible for the recent reduced carbon sink strength. In this study, we used a record of nearly 100 site-years of eddy covariance …

Globally Scalable Approach To Estimate Net Ecosystem Exchange Based On Remote Sensing, Meteorological Data, And Direct Measurements Of Eddy Covariance Sites, Ruslan Zhuravlev, Andrey Dara, André Luís Diniz Dos Santos, Oleg Demidov, George Burba

Daugherty Water for Food Global Institute: Faculty Publications

Despite a rapid development of Nature-Based Solutions (NBS) for carbon removal in recent years, the methods for evaluating NBS still have certain gaps. We propose an approach based on a combination of remote sensing data and meteorological variables to reconstruct the spatiotemporal variation of net ecosystem exchange from eddy-covariance stations. A Lagrangian particle dispersion model was used for upscaling satellite images and flux towers. We trained data-driven models based on kernel methods separately for each selected land-cover class. The results suggest that the proposed approach to quantifying carbon exchange on a medium-to-large scale by blending eddy covariance flux data with …

Role Of Social Determinants Of Health In Differential Respiratory Exposure And Health Outcomes Among Children, Jagadeesh Puvvula, Jill A. Poole, Yeongjin Gwon, Eleanor G. Rogan, Jesse E. Bell

Daugherty Water for Food Global Institute: Faculty Publications

Background Attributes defining the Social Determinants of Health (SDoH) are associated with disproportionate exposures to environmental hazards and differential health outcomes among communities. The dynamics between SDoH, disproportionate environmental exposures, and differential health outcomes are often specific to micro-geographic areas.

Methods This study focused on children less than 20 years of age who lived in Douglas County, Nebraska, during 2016–2019. To assess the role of SDoH in differential exposures, we evaluated the association between SDoH metrics and criteria pollutant concentrations and the association between SDoH and pediatric asthma exacerbations to quantify the role of SDoH in differential pediatric asthma outcomes. …

Labile Carbon And Soil Texture Control Nitrogen Transformation In Deep Vadose Zone, Lidong Li, Jordan Shields, Daniel D. Snow, Michael Kaiser, Arindam Malakar

Daugherty Water for Food Global Institute: Faculty Publications

Understanding transient nitrogen (N) storage and transformation in the deep vadose zone is critical for controlling groundwater contamination by nitrate. The occurrence of organic and inorganic forms of carbon (C) and nitrogen and their importance in the deep vadose zone is not well characterized due to difficulty in sampling and the limited number of studies. We sampled and characterized these pools beneath 27 croplands with different vadose zone thicknesses (6–45 m). We measured nitrate and ammonium in different depths for the 27 sites to evaluate inorganic N storage. We measured total Kjeldahl nitrogen (TKN), hot-water extractable organic carbon (EOC), soil …

Development Of A Framework To Support Community-Scale Nutrient Recovery For Local Crop Fertilization And Production, Scott A. Lopez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nutrient and resource recovery (NRR) has become increasingly crucial as regions face deteriorating sanitation infrastructures, limited support, and growing environmental challenges. Implementing region-specific NRR technologies and systems could provide effective circular economy insights into addressing these challenges. Because of the multidisciplinary challenges associated with the design and implementation of NRR strategies, various government and local decision-makers need to collaborate effectively in the decision-making process. Structured decision-making (SDM) methodologies are practical when determining the most appropriate NRR strategy for nutrient-rich waste streams. However, applications of computational SDM limited because of the vast amounts of data that are needed to analyze the …

Ex-Ante Cost-Benefit Analysis Of High-End And Low-Cost Wireless Sensor Network (Wsn) Technology Packages For Efficient Irrigation Water Management In The Philippines, Marielle Q. Aringo, Victor B. Ella, Camille G. Martinez, Gamiello S. Pereira

Journal of Economics, Management and Agricultural Development

Four wireless sensor network (WSN) technology packages developed for efficient irrigation water management in the Philippines were subjected to ex-ante cost-benefit analysis (CBA) to assess their financial viability. The WSN technologies include high-end and low-cost wireless sensors for upland crop production with drip irrigation system and lowland crop production with alternate wetting and drying (AWD). Results showed that the high-end WSN technology packages are only viable for high-value crops such as red onion, bell pepper, and hot pepper. The low-cost WSN technology packages are viable for all selected crops except sweet corn. Minimum areas were also generated for each crop …

Earlier Snowmelt May Lead To Late Season Declines In Plant Productivity And Carbon Sequestration In Arctic Tundra Ecosystems, Donatella Zona, Peter M. Lafleur, Koen Hufkens, Barbara Bailey, Beniamino Gioli, George Burba, Jordan P. Goodrich, Anna K. Liljedahl, Eugénie S. Euskirchen, Jennifer D. Watts, Mary Farina, John S. Kimball, Martin Heimann, Mathias Göckede, Martijn Pallandt, Torben R. Christensen, Mikhail Mastepanov, Efrén López-Blanco, Marcin Jackowicz-Korczynski, Albertus J. Dolman, Luca Belelli Marchesini, Roisin Commane, Steven C. Wofsy, Charles E. Miller, David A. Lipson, Josh Hashemi, Kyle A. Arndt, Lars Kutzbach, David Holl, Julia Boike, Christian Wille, Torsten Sachs, Aram Kalhori, Xia Song

Daugherty Water for Food Global Institute: Faculty Publications

Arctic warming is affecting snow cover and soil hydrology, with consequences for carbon sequestration in tundra ecosystems. The scarcity of observations in the Arctic has limited our understanding of the impact of covarying environmental drivers on the carbon balance of tundra ecosystems. In this study, we address some of these uncertainties through a novel record of 119 site-years of summer data from eddy covariance towers representing dominant tundra vegetation types located on continuous permafrost in the Arctic. Here we found that earlier snowmelt was associated with more tundra net CO2 sequestration and higher gross primary productivity (GPP) only in June …

Permafrost Landscape History Shapes Fluvial Chemistry, Ecosystem Carbon Balance, And Potential Trajectories Of Future Change, Scott Zolkos, Suzanne E. Tank, Steven V. Kokelj, Robert G. Striegl, Sarah Shakil, Carolina Voigt, Oliver Sonnentag, William L. Quinton, Edward A.G. Schuur, Donatella Zona, Peter M. Lafleur, Ryan C. Sullivan, Masahito Ueyama, David Billesbach, David Cook, Elyn R. Humphreys, Philip Marsh

Daugherty Water for Food Global Institute: Faculty Publications

Intensifying permafrost thaw alters carbon cycling by mobilizing large amounts of terrestrial substrate into aquatic ecosystems. Yet, few studies have measured aquatic carbon fluxes and constrained drivers of ecosystem carbon balance across heterogeneous Arctic landscapes. Here, we characterized hydrochemical and landscape controls on fluvial carbon cycling, quantified fluvial carbon fluxes, and estimated fluvial contributions to ecosystem carbon balance across 33 watersheds in four ecoregions in the continuous permafrost zone of the western Canadian Arctic: unglaciated uplands, ice-rich moraine, and organic-rich lowlands and till plains. Major ions, stable isotopes, and carbon speciation and fluxes revealed patterns in carbon cycling across ecoregions …

Uranium 233: The Nuclear Superfuel No One Is Using, Maris Hanson

Seattle Journal of Technology, Environmental, & Innovation Law

Nuclear power offers more energy in less physical space than solar and wind and yields more energy per pound than fossil fuels. However different nuclear fuels yield different waste profiles and create different beneficial products. Uranium 233 (U233) resists use in nuclear weapons, yields beneficial daughter products, and produces dramatically less of the most problematic waste products than Uranium 235 (U235). U233 results from reactions with Thorium, a plentiful, ubiquitous element currently considered waste from rare earth mines. Additionally, U233 functions well in a liquid fuel reactor resulting in safer, more efficient reactors than current solid fuel U235 or Plutonium …

Contested Landscapes, Disputed Realities: An Investigation Of Socio-Environmental Conflict From Mining In Northern Ecuador: An Honors Thesis, Ian F. Hirons

Student Works

For decades, Ecuador has been one of the preeminent petrostates in South America. However, in response to recent drops in global demand and pricing for oil products, the country has made serious commitments to further develop its mineral resources. By opening a new natural resource sector, Ecuador has firmly cemented itself as a primarily extractivist nation. In the process, the national government has frequently come into conflict with activist and community groups who protest the encroachment of extractive industry. This thesis explores the various dimensions of socio-environmental conflict created by large-scale mining projects in northern Ecuador with specific attention to …

Optimizing Work Zone Schedules Considering Traffic Diversion With Artificial Bee Colony Algorithm, Celina Semaan

Dissertations

Highway maintenance activities often decrease roadway capacity and intrude traffic movements. The need to finish the project on time and under a specific budget while minimizing the traffic congestion and complying with the emission standards requires an appropriate work zone schedule optimization. The objective of this research is to improve the efficiency of work zone activities and minimize the total project cost including maintenance, user, and emission cost.

While previous studies investigated the work zone optimization problem, they did not consider the implementation of emission standards nor applied a green diversion strategy. This dissertation analyzes the optimization of work zone …

A Systematic Review Of The Use Of Environmental Economics In The Mining Industry, Maria Menegaki, Dimitris Damigos

Journal of Sustainable Mining

Environmental economics is increasingly being used in project appraisals, environmental liability estimates and design of market-based instruments. Mining, an actor capable of causing adverse effects on the environment, human health and well-being, has already been affected by these developments, at a great extent. Up to date, several research studies have been carried out to monetise the externalities of mining projects. Nevertheless, a systematic review of these publications has not been carried out, yet. This paper aims to fill this gap in the literature by investigating (i) the main non-market valuation techniques used; (ii) the main external costs or benefits of …

Moving Beyond ‘More Crop Per Drop’: Insights From Two Decades Of Research On Agricultural Water Productivity, Meredith Giordano, Susanne M. Scheierling, David O. Tréguer, Hugh Turral, Peter G. Mccornick

Daugherty Water for Food Global Institute: Faculty Publications

Concern over increasing water scarcity has led to the introduction of the concept of agricultural water productivity and an emphasis on interventions to achieve ‘more crop per drop’. Yet, a strong debate continues on how the concept is to be defined and used. Drawing largely from the irrigation literature, the origins of the concept and its methodological developments are reviewed, and its use in applied work over two decades is discussed. Based on this analysis of conceptual and applied research, key insights into the concept’s contributions and limitations are presented, as well as opportunities for further refinements.

Nanomaterials In The Environment, Human Exposure Pathway, And Health Effects: A Review, Arindam Malakar, Sushil R. Kanel, Chittaranjan Ray, Daniel D. Snow, Mallikarjuna N. Nadagouda

Daugherty Water for Food Global Institute: Faculty Publications

Nanomaterials (NMs), both natural and synthetic, are produced, transformed, and exported into our environment daily. Natural NMs annual flux to the environment is around 97% of the total and is significantly higher than synthetic NMs. However, synthetic NMs are considered to have a detrimental effect on the environment. The extensive usage of synthetic NMs in different fields, including chemical, engineering, electronics, and medicine, makes them susceptible to be discharged into the atmosphere, various water sources, soil, and landfill waste. As ever-larger quantities of NMs end up in our environment and start interacting with the biota, it is crucial to understand …

Baseflow Nitrate Dynamics Within Nested Watersheds Of An Agricultural Stream In Nebraska, Usa, Galen Richards, Troy E. Gilmore, Aaron R. Mittelstet, Tiffany Messer, Daniel D. Snow

Daugherty Water for Food Global Institute: Faculty Publications

There is a need to evaluate high surface nitrate concentrations across agricultural watersheds, both spatially and temporally, to increase understanding of source and timing of nitrogen loads in streams and rivers. Bazile Creek is a high-nitrate stream originating in the agriculturally intensive Bazile Groundwater Management Area of Eastern Nebraska, USA. It is a gaining stream that receives groundwater with high nitrate concentrations originating from nonpoint sources. The objective of this study was to determine spatial and temporal variability of baseflow nitrate concentrations in Bazile Creek and its tributaries and to relate this variability to watershed characteristics. Surface-water nitrate samples were …

Surveillance Of Plasticizers, Bisphenol A, Steroids And Caffeine In Surface Water Of River Ganga And Sundarban Wetland Along The Bay Of Bengal: Occurrence, Sources, Estrogenicity Screening And Ecotoxicological Risk Assessment, Paromita Chakraborty, Nancy W. Shappell, Moitraiyee Mukhopadhyay, Sathaporn Onanong, Daniel D. Snow

Daugherty Water for Food Global Institute: Faculty Publications

The transboundary River Ganga serves as a conduit for meltwater from the Himalayas and is a major freshwater source for two thirds of Indian population before emptying into the Sundarban Delta, the largest estuary in the Bay of Bengal. Endocrine disrupting compounds (EDCs) such as phthalic acid esters (PAEs) and bisphenol A (BPA) used as organic plastic additives can pollute the aquatic environment receiving plastic litter. Hence, we have investigated these EDCs in water samples from Ganga and Sundarban wetland of India. Since these compounds exhibit estrogenic potential, we have further measured steroids and evaluated the estrogenic activity (estradiol equivalents, …

Transmission Routes Of The Microbiome And Resistome From Manure To Soil And Lettuce, Yuepeng Sun, Daniel D. Snow, Harkamal Walia, Xu Li

Daugherty Water for Food Global Institute: Faculty Publications

The land application of animal manure can introduce manure microbiome and resistome to croplands where food crops are grown. The objective of this study was to characterize the microbiome and resistome on and in the leaves of lettuce grown in manured soil and identify the main transmission routes of microbes and antibiotic resistance genes (ARGs) from soil to the episphere and endosphere of lettuce. Shotgun metagenomic results show that manure application significantly altered the composition of the microbiome and resistome of surface soil. SourceTracker analyses indicate that manure and original soil were the main source of the microbiome and resistome …