Biogeochemistry Commons^™

Interactions Between Changing Pco2, N2 Fixation, And Fe Limitation In The Marine Unicellular Cyanobacterium Crocosphaera, Fei-Xue Fu, Margaret R. Mulholland, Nathan S. Garcia, Aaron Beck, Mark E. Warner, Sergio A. Sañudo, David A. Hutchins

OES Faculty Publications

We examined the physiological responses of steady-state iron (Fe)-replete and Fe-limited cultures of the biogeochemically critical marine unicellular diazotrophic cyanobacterium Crocosphaera at glacial (19 Pa; 190 ppm), current (39 Pa; 380 ppm), and projected year 2100 (76 Pa; 750 ppm) CO₂ levels. Rates of N₂ and CO₂ fixation and growth increased in step with increasing partial pressure of CO₂ (pCO₂), but only under Fe- replete conditions. N₂) and carbon fixation rates at 75 Pa CO₂ were 1.4-1.8-fold and 1.2-2.0-fold higher, respectively, relative to those at present day and glacial pCO_{2 …}

Nutrient And Carbon Dynamics In The Chesapeake Bay Outflow Plume And Their Effect On The Coastal Ocean Environment, Katherine C. Filippino

OES Theses and Dissertations

Seasonally resolved nutrient and carbon fluxes from estuaries to the coastal ocean are poorly constrained. Nutrient and carbon cycling in highly productive regions like the Chesapeake Bay outflow plume and surrounding coastal environments greatly affect our global understanding of carbon cycling. The overall questions for the research described in this dissertation stem from the need to close global carbon budgets, and obtain a fundamental understanding of nutrient dynamics in a coastal region heavily influenced by seasonality and human impacts.

Within the framework of physical characteristics of the outflow plume and through the characterization of nutrient concentrations, primary productivity rates, and …

The Observation, Modeling, And Retrieval Of Bio-Optical Properties For Coastal Waters Of The Southern Chesapeake Bay, Xiaoju Pan

OES Theses and Dissertations

The primary purpose of this study was to develop an inverse method to retrieve the inherent optical properties (IOPs) and biogeochemical parameters (e.g. chlorophyll a concentration and salinity) appropriate to monitor the water quality and biogeochemical processes from remote sensing of the coastal waters in the southern Chesapeake Bay and coastal Mid-Atlantic Bight region (MAB) dominated by Case 2 waters. For this purpose, knowledge of the relationship between remote sensing reflectance (R_rs) and IOPs and the effect from bottom reflectance on R_rs, is required.

A substantial investigation of IOPs has been conducted for the coastal …

Assessment Of Skill And Portability In Regional Marine Biogeochemical Models: Role Of Multiple Planktonic Groups, Marjorie A. M. Friedrichs, Jeffery A. Dusenberry, Laurence A. Anderson, Robert A. Armstrong, Fei Chai, James R. Christian, Scott C. Doney, John Dunne, Masahiko Fujii, Jerry D. Wiggert

CCPO Publications

[1] Application of biogeochemical models to the study of marine ecosystems is pervasive, yet objective quantification of these models' performance is rare. Here, 12 lower trophic level models of varying complexity are objectively assessed in two distinct regions (equatorial Pacific and Arabian Sea). Each model was run within an identical one-dimensional physical framework. A consistent variational adjoint implementation assimilating chlorophyll-a, nitrate, export, and primary productivity was applied and the same metrics were used to assess model skill. Experiments were performed in which data were assimilated from each site individually and from both sites simultaneously. A cross-validation experiment was also conducted …

Impact Of Anthropogenic Combustion Emissions On The Fractional Solubility Of Aeroosol Iron: Evidence From The Sargasso Sea, Peter N. Sedwick, Edward R. Sholkovitz, Thomas M. Church

OES Faculty Publications

We report empirical estimates of the fractional solubility of aerosol iron over the Sargasso Sea during periods characterized by high concentrations of Saharan dust (summer 2003) and by low concentrations of aerosols in North American/maritime North Atlantic air masses (spring 2004 and early summer 2004). We observed a strong inverse relationship between the operational solubility of aerosol iron (defined using a flow-through deionized-water leaching protocol) and the total concentration of aerosol iron, whereby the operational solubility of aerosol iron was elevated when total aerosol iron loadings were low. This relationship is consistent with source-dependent differences in the solubility characteristics of …

Simulations Of Phytoplankton Species And Carbon Production In The Equatorial Pacific Ocean 2. Effects Of Physical And Biogeochemical Processes, Baris Salihoglu, Eileen E. Hofmann

CCPO Publications

A one-dimensional multi-component lower trophic level ecosystem model that includes detailed algal physiology is used to investigate the response of phytoplankton community and carbon production and export to variations in physical and biochemical processes in the Cold Tongue region of the equatorial Pacific Ocean at ON, 140W. Results show that high-frequency variability in vertical advection and temperature is an important mechanism driving the carbon export. Filtering out low frequency physical forcing results in a 30% increase in primary production and dominance of high-light adapted Prochlorococcus and autotrophic eukaryotes. Sensitivity studies show that iron availability is the primary control on carbon …

The Fate Of Nitrogren Fixed By Diazotrophs In The Ocean, Margaret R. Mulholland

OES Faculty Publications

While we now know that N₂ fixation is a significant source of new nitrogen (N) in the marine environment, little is known about the fate of this N (and associated C), despite the importance of diazotrophs to global carbon and nutrient cycles. Specifically, does N fixed during N₂ fixation fuel autotrophic or heterotrophic growth and thus facilitate carbon (C) export from the euphotic zone, or does it contribute primarily to bacterial productivity and respiration in the euphotic zone? For Trichodesmium, the diazotroph we know the most about, the transfer of recently fixed N₂ (and C) appears …

Co2 Control Of Trichodesmium N-2 Fixation, Photosynthesis, Growth Rates, And Elemental Ratios: Implications For Past, Present, And Future Ocean Biogeochemistry, D. A. Hutchins, F.-X. Fu, Y. Zhang, M. E. Warner, Y. Feng, K. Portune, P. W. Bernhardt, M. R. Mulholland

OES Faculty Publications

Diazotrophic marine cyanobacteria in the genus Trichodesmium contribute a large fraction of the new nitrogen entering the oligotrophic oceans, but little is known about how they respond to shifts in global change variables such as carbon dioxide (CO₂) and temperature. We compared Trichodesmium dinitrogen (N₂) and CO₂ fixation rates during steady-state growth under past, current, and future CO₂ scenarios, and at two relevant temperatures. At projected CO₂ levels of year 2100 (76 Pa, 750 ppm), N₂ fixation rates of Pacific and Atlantic isolates increased 35-100%, and CO₂ fixation rates increased 15-128% …

Marine And Freshwater Cyanophages In A Laurentian Great Lake: Evidence From Infectivity Assays And Molecular Analyses Of G20 Genes, Steven W. Wilhelm, Matthew J. Carberry, Melanie L. Eldridge, Leo Poorvin, Matthew A. Saxton, Martina A. Doblin

OES Faculty Publications

While it is well established that viruses play an important role in the structure of marine microbial food webs, few studies have directly addressed their role in large lake systems. As part of an ongoing study of the microbial ecology of Lake Erie, we have examined the distribution and diversity of viruses in this system. One surprising result has been the pervasive distribution of cyanophages that infect the marine cyanobacterial isolate Synechococcus sp. strain WH7803. Viruses that lytically infect this cyanobacterium were identified throughout the western basin of Lake Erie, as well as in locations within the central and eastern …

Biogeochemistry Of Arsenic And Antimony In The North Pacific Ocean, Gregory A. Cutter, Lynda S. Cutter

OES Faculty Publications

The biogeochemical cycles of the metalloid elements arsenic and antimony were examined along a 15,000 km surface water transect and at 9 vertical profile stations in the western North Pacific Ocean as part of the 2002 IOC Contaminant Baseline Survey. Results show that the speciation of dissolved arsenic (As III, As V, and methylated As) was subtly controlled by the arsenate (AsV)/phosphate ratio. An additional fraction of presumed organic arsenic previously reported in coastal waters was also present (~15% of the total As) in oceanic surface waters. Dissolved inorganic antimony displayed mildly scavenged behavior that was confirmed by correlations with …

Hydrographic Observations During The 2002 Ioc Contaminant Baseline Survey In The Western Pacific Ocean, C. I. Measures, Greg A. Cutter, W. M. Landing, R. T. Powell

OES Faculty Publications

The 2002 IOC Contaminant Baseline Survey in the western Pacific Ocean was the fourth in a series of cruises intended to establish the contemporary concentrations of trace elements and other materials in the major water masses of the ocean and to illuminate the pathways by which materials delivered to the surface ocean are incorporated in the subsurface waters. The expedition occupied 9 vertical profile stations encompassing the subtropical and subarctic gyre of the western North Pacific. In addition, underway surface water samples were collected during transits between the stations. This paper uses the temperature, salinity, nutrient, oxygen, and chlorophyll data …

Pelagic Functional Group Modeling: Progress, Challenges And Prospects, Raleigh R. Hood, Edward A. Laws, Robert A. Armstrong, Nicholas R. Bates, Christopher W. Brown, Craig A. Carlson, Fei Chai, Scott C. Doney, Paul G. Falkowski, Richard A. Feely, Marjorie A. M. Friedrichs

CCPO Publications

In this paper, we review the state of the art and major challenges in current efforts to incorporate biogeochemical functional groups into models that can be applied on basin-wide and global scales, with an emphasis on models that might ultimately be used to predict how biogeochemical cycles in the ocean will respond to global warming. We define the term "biogeochemical functional group" to refer to groups of organisms that mediate specific chemical reactions in the ocean. Thus, according to this definition, "functional groups" have no phylogenetic meaning-these are composed of many different species with common biogeochemical functions.

Substantial progress has …

Evaluating The Biogeochemical Cycle Of Selenium In San Francisco Bay Through Modeling, Shannon L. Meseck, Gregory A. Cutter

OES Faculty Publications

A biogeochemical model was developed to simulate salinity, total suspended material, phytoplankton biomass, dissolved selenium concentrations (selenite, selenate, and organic selenide), and particulate selenium concentrations (selenite + selenate, elemental selenium, and organic selenide) in the San Francisco Bay estuary. Model-generated estuarine profiles of total dissolved selenium reproduced observed estuarine profiles at a confidence interval of 91- 99% for 8 different years under various environmental conditions. The model accurately reproduced the observed dissolved speciation at confidence intervals of 81-98% for selenite, 72-91% for selenate, and 60-96% for organic selenide. For particulate selenium, model-simulated estuarine profiles duplicated the observed behavior of total …

Iron In Ice Cores From Law Dome: A Record Of Atmospheric Iron Deposition For Maritime East Antarctica During The Holocene And Last Glacial Maximum, Ross Edwards, Peter N. Sedwick, Vin Morgan, Claude Boutron

OES Faculty Publications

Total dissolvable iron (TDFe) was measured in sections of ice cores recovered from Law Dome on the coast of Wilkes Land, East Antarctica. These samples include ice dating from the Last Glacial Maximum (LGM), the Last Deglaciation, and the early and mid Holocene as well as samples from the Anthropocene that have been dated with seasonal to annual resolution. Combining our TDFe concentration data with estimates of the ice accumulation rate, we estimate the atmospheric iron deposition for Law Dome and the adjacent Southern Ocean during these periods. Our results indicate that the atmospheric iron deposition flux to this region …

Nitrogen Fixation And Release Of Fixed Nitrogen By Trichodesmium Spp. In The Gulf Of Mexico, Margaret R. Mulholland, Peter W. Bernhardt, Cynthia A. Heil, Deborah A. Bronk, Judith M. O'Neil

OES Faculty Publications

During a 3-yr study in the Gulf of Mexico, we measured dinitrogen (N₂) fixation and nitrogen (N) release by Trichodesmium and compared these rates with water column N demand and the estimated N necessary to support blooms of Karenia brevis, a toxic dinoflagellate that severely affects the West Florida shelf. Net and gross N₂ fixation rates were compared in simultaneous incubations using ¹⁵N₂ uptake and acetylene reduction, respectively. The difference between net and gross N₂ fixation is assumed to be an approximation of the rate of N release. Results demonstrate that Trichodesmium in …

Monsoon-Driven Biogeochemical Processes In The Arabian Sea, Jerry D. Wiggert, Raleigh Hood, Karl Banse, John Kindle

Faculty Publications

Although it is nominally a tropical locale, the semiannual wind reversals associated with the Monsoon system of the Arabian Sea result annually in two distinct periods of elevated biological activity. While in both cases monsoonal forcing drives surface layer nutrient enrichment that supports increased rates of primary productivity, fundamentally different entrainment mechanisms are operating in summer (Southwest) and winter (Northeast) Monsoons. Moreover, the intervening intermonsoon periods, during which the region relaxes toward oligotrophic conditions more typical of tropical environments, provide a stark contrast to the dynamic biogeochemical activity of the monsoons. The resulting spatial and temporal variability is great and …

Burial Of Terrestrial Organic Matter In Marine Sediments: A Re-Assessment, David J. Burdige

OES Faculty Publications

Calculations based on recent observations indicate that approximately one third of the organic matter presently being buried in marine sediments may be of terrestrial origin, with the majority of this terrestrial organic matter (TOM) burial occurring in muddy, deltaic sediments. These calculations further suggest that the remineralization of terrestrial organic matter in the oceans is also much less efficient than that of marine organic matter. These two underappreciated observations have important implications in terms of our understanding of the controls on the global carbon cycle. From a paleoceanographic perspective, the results presented here also suggest that changes in TOM burial …

Iron In The Sargasso Sea (Bermuda Atlantic Time-Series Study Region) During Summer: Eolian Imprint, Spatiotemporal Variability, And Ecological Implications, Peter N. Sedwick, T. M. Church, A. R. Bowie, C. M. Marsay, S. J. Ussher, K. M. Achilles, P. J. Lethaby, R. J. Johnson, M. M. Sarin, D. J. Mcgillicuddy

OES Faculty Publications

We report iron measurements for water column and aerosol samples collected in the Sargasso Sea during July-August 2003 (summer 2003) and April-May 2004 (spring 2004). Our data reveal a large seasonal change in the dissolved iron (dFe) concentration of surface waters in the Bermuda Atlantic Time-series Study region, from ∼1-2nM in summer 2003, when aerosol iron concentrations were high (mean 10 nmol/m^-3), to ∼0.1-0.2nM in spring 2004, when aerosol iron concentrations were low (mean 0.64 nmol/m^-3). During summer 2003, we observed an increase of ∼0.6nM in surface water dFe concentrations over 13 days, presumably due to …

A Unique Seasonal Pattern In Phytoplankton Biomass In Low-Latitude Waters In The South China Sea, Chun-Mao Tseng, George T. F. Wong, I.-I. Lin, C.-R. Wu, K.-K. Liu

OES Faculty Publications

A distinctive seasonal pattern in phytoplankton biomass was observed at the South East Asian Time series Study (SEATS) station (18°N, 116°E) in the northern South China Sea (SCS). Surface chlorophyll-a, depth integrated chlorophyll-a and primary production were elevated to 0.3 mg/m³, ~35 mg/m² and 300 mg-C/m²/d, respectively, in the winter but stayed low, at 0.1 mg/m³, ~15 mg/m² and 110 mg-C/m²/d as commonly found in other low latitude waters, in the rest of the year. Concomitantly, soluble reactive phosphate and nitrate+nitrite in the mixed layer also became …

Hydrographic And Biological Changes In The Taiwan Strait During The 1997-1998 El Niño Winter, S. Shang, C. Zhang, H. Hong, Q. Liu, G. T. F. Wong, C. Hu, B. Huang

OES Faculty Publications

During the 1997–1998 El Niño event, the average sea surface temperature (SST) in the Taiwan Strait (TWS) in the winter was ~1.4°C higher than that of the winter climatological mean. The areal ratio of the warm water (≥2°C above the regional mean) to the cold water (≥2°C below the regional mean) in the TWS increased by 25% while the area of the eutrophic water (chlorophyll a >1 mg m^-3) was halved. Field observations also indicate that the mixed layer in the TWS became more nutrient-poor during this winter. These observations are consistent with a diminished advection of the …

A Watershed-Based Classification System For Lakes In Agriculturally-Dominated Ecosystems: A Case Study Of Nebraska Reservoirs, Henry N. N. Bulley

Department of Geography: Dissertations, Theses, and Student Research

In recent decades substantial progress has been made in improving the quality of surface waters in the United States (Hawkins et al., 2000; EPA, 2000; EPA, 2001); nevertheless, much work remains to be done in assessing the state of impairment of lake waters. Impairment implies that the existing water quality of a lake, as measured by selected criteria (e.g., nitrogen, phosphorus, chlorophyll-a, Secchi depth), exceeds a threshold value or standard that presumably reflects optimal attainable lake water quality conditions (or "reference" conditions) (Hughes, 1995; EPA, 2000; EPA, 2001). Such impaired waters are not suitable for designated uses such as drinking, …

Estimation Of Primary Production And Carbon Flux In Antarctic Coastal Waters: A Modeling Study, Hae-Cheol Kim

OES Theses and Dissertations

This study presents results from models that are designed to simulate the underwater light field, to simulate phytoplankton primary production, and to estimate the fate of phytoplankton carbon in continental shelf waters of the west Antarctic Peninsula (WAP) and Ross Sea. Simulation of the underwater light field required derivation of new coefficient sets for power function-type cloud cover correction algorithms, which were found to be influenced by multiple reflections between the bottom of clouds and the surface. The coefficient sets indicate that the spectral effect of clouds on the properties of the surface irradiance was spectrally-neutral for wavelengths greater than …

Sources And Cycling Of Carbonyl Sulfide In The Sargasso Sea, Gregory A. Cutter, Lynda S. Cutter, Katherine C. Filippino

OES Faculty Publications

The cycling of the radiatively important gas carbonyl sulfide (OCS) was studied in surface waters of the Sargasso Sea. In August 1999, surface OCS concentrations averaged 8.6 pmol L^-1, showed minor diel variations, and varied little with depth. An OCS precursor, total dissolved organic sulfur (DOS), was lowest at the surface (40 nmol L^-1) and increased with depth. The photoproduction rate of OCS from in situ incubations averaged 9.6 pmol L^-1 h^-1, whereas dark production was 7.0 pmol L^-1 h^-1. Apparent quantum yields were 10^-5-10^-7 from 313-436 …

Analytical Intercomparison Between Flow Injection-Chemiluminescence And Flow Injection-Spectrophotometry For The Determination Of Picomolar Concentrations Of Iron In Seawater, Andrew R. Bowie, Peter N. Sedwick, Paul J. Worsfold

OES Faculty Publications

A lab- and ship-based analytical intercomparison of two flow injection methods for the determination of iron in seawater was conducted, using three different sets of seawater samples collected from the Southern Ocean and South Atlantic. In one exercise, iron was determined in three different size-fractions (< 0.03 &μm, < 0.4 μm, and unfiltered) in an effort to better characterize the operational nature of each analytical technique with respect to filter size. Measured Fe concentrations were in the range 0.19 to 1.19 nM using flow injection with luminol chemiluminescence detection (FI-CL), and 0.07 to 1.54 nM using flow injection with catalytic spectrophotometric detection with N, N-dimethyl-p-phenylenediamine dihydrochloride (FI-DPD). The arithmetic mean for the FI-CL method was higher (by 0.09 nM) than the FI-DPD method for dissolved (< 0.4 μm) Fe, a difference that is comparable to the analytical blanks, which were as high as 0.13 nM ( CL) and 0.09 nM (DPD). There was generally good agreement between the FI-CL determinations for the < 0.03 μm size fraction and the FI-DPD determinations for the < 0.4 μm size fraction in freshly collected samples. Differences in total-dissolvable ( unfiltered) Fe concentrations determined by the two FI methods were more variable, reflecting the added complexity associated with the analysis of partially digested particulate material in these samples. Overall, however, the FI-CL determinations were significantly (P = 0.05) lower than the FI-DPD determinations for the unfiltered samples. Our results suggest that the observed, systematic inter-method differences reflect measurement of different physicochemical fractions of Fe present in seawater, such that colloidal and/or organic iron species are better determined by the FI-CL method than the FI-DPD method. This idea is supported by our observation that inter-method differences were largest for freshly collected acidified seawater, which suggests extended storage (>6 months) of acidified samples as a possible protocol for the determination of dissolved iron in seawater.

Black Carbon In Estuarine And Coastal Ocean Dissolved Organic Matter, Antonio Mannino, H. Rodger Harvey

OES Faculty Publications

We measured black carbon (BC) in ultrafiltered, high-molecular weight dissolved organic matter (UDOM) in surface waters of Delaware Bay, Chesapeake Bay, and the adjacent Atlantic Ocean (U.S.A.) to investigate the importance of riverine and estuarine dissolved organic matter (DOM) as a source of BC to the ocean. BC was 5-72% of UDOM-C (27 ± 17%), which corresponds to 8.9 ± 6.5% of dissolved organic carbon (DOC), with higher values in the heavily urbanized midbay region of the Delaware Estuary and lower yields in the river and coastal ocean. The spatial and seasonal distributions of BC along the salinity gradient of …

An Investigation Of Dissolved Organic Matter In A Shallow Coastal Bay Subject To Aureococcus Anophagefferens Blooms, Jean-Paul Simjouw

OES Theses and Dissertations

Aureococcus anophagefferens, the pelagophyte responsible for brown tide blooms, was identified in Chincoteague Bay in 1997 and has “bloomed” there since at least 1998. Aureococcus anophagefferens is capable of using dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) substrates to support growth, and this utilization is hypothesized to give the organism a competitive advantage relative to other phytoplankton when inorganic nutrient concentrations are low or depleted. Because previous studies suggest dissolved organic matter (DOM) is important in initiating and sustaining brown tide blooms, a field study of the variations in DOC concentration and DOM composition was performed at …

New Evidence For Enhanced Ocean Primary Production Triggered By Tropical Cyclone, I. Lin, W. Timothy Liu, Chun-Chieh Wu, George T. F. Wong, Chuanmin Hu, Zhiqiang Chen, Wen-Der Liang, Yih Yang, Kon-Kee Liu

OES Faculty Publications

[1] New evidence based on recent satellite data is presented to provide a rare opportunity in quantifying the long-speculated contribution of tropical cyclones to enhance ocean primary production. In July 2000, moderate cyclone Kai-Tak passed over the South China Sea (SCS). During its short 3-day stay, Kai-Tak triggered an average 30-fold increase in surface chlorophyll-a concentration. The estimated carbon fixation resulting from this event alone is 0.8 Mt, or 2-4% of SCS's annual new production. Given an average of 14 cyclones passing over the SCS annually, we suggest the long-neglected contribution of tropical cyclones to SCS's annual new production may …

Nitrate Anomaly In The Upper Nutricline In The Northern South China Sea - Evidence For Nitrogen Fixation, George T. F. Wong, Shi-Wei Chung, Fuh-Kwo Shiah, Chung-Chi Chen, Liang-Saw Wen, Kon-Kee Liu

OES Faculty Publications

[1] Up to 2 μM of nitrate anomaly, N*, were found in the upper nutricline at the South East Asia Time-series Study (SEATS) site in the northern South China Sea (SCS). These concentrations were among the higher values reported in the Pacific and indicate the significant contribution of the remineralization of nitrogen-rich organic matter formed by nitrogen fixation to the nutrient dynamics of the area. The concentrations were systematically higher, by up to 2.5 μM, in the Fall through the early Spring, during the northeast monsoon, than in the Summer, suggesting that the impact of nitrogen fixation was higher during …

Modeling The Biogeochemical Cycle Of Selenium In The San Francisco Bay, Shannon L. Meseck

OES Theses and Dissertations

Due to recent concerns about selenium toxicity in the San Francisco Bay and the roles of refinery and San Joaquin River inputs on the selenium cycle, the model ECoS 3 (distributed from Plymouth Marine Laboratory, United Kingdom) was modified to simulate the biogeochemical cycle of selenium in the Northern Reach. The model is designed to simulate salinity, total suspended material, phytoplankton concentrations, dissolved selenium and its speciation (selenite, selenate, and organic selenide), and particulate selenium and its speciation (selenite + selenate, elemental selenium, and organic selenide). Actual data from 1999 were used to calibrate the model, while data from other …

Dark Production: A Significant Source Of Oceanic Cos, M. Von Hobe, Gregory A. Cutter, A. J. Kettle, M. O. Andreae

OES Faculty Publications

Carbonyl sulfide (COS) in air and dissolved in seawater was determined during a cruise in August 1999 in the Sargasso Sea in the northwest Atlantic Ocean. Dissolved concentrations at the sea surface displayed only a weak diel cycle with a mean of 8.6 ± 2.8 pmol dm⁻³ owing to low abundance of photochemical precursors and high temperatures causing rapid hydrolysis. Depth profiles measured over the oceanic mixed layer revealed significant vertical gradients of COS concentration with higher values at the surface, suggesting that the rate of photochemical production at the surface exceeds the rate of vertical mixing. The mean …