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Full-Text Articles in Medicinal-Pharmaceutical Chemistry
Development Of Entrapment Columns For The Study Of Affinity Based Analysis Of Drug-Protein Interactions, Shiden T. Azaria
Development Of Entrapment Columns For The Study Of Affinity Based Analysis Of Drug-Protein Interactions, Shiden T. Azaria
Department of Chemistry: Dissertations, Theses, and Student Research
High-performance affinity chromatography (HPAC) is a type of liquid chromatography in which solutes are separated based on their binding to a stationary phase that is a biologically-related agent. Because of the strong and selective nature of many biological interactions, this method has already become a powerful technique for the purification and analysis of solutes that are complementary to the immobilized binding agent. Human serum albumin (HSA), the most abundant protein in the blood with concentrations of 35-50 mg/mL in serum, has interactions with many drugs, which can affect the absorption, distribution, metabolism and excretion of such agents.
The overall goal …