Inorganic Chemistry Commons^™

Nanoparticle Film Assemblies As Platforms For Electrochemical Biosensing – Factors Affecting Amperometric Signal Enhancement Of Hydrogen Peroxide, Adrienne R. Schmidt, Natalie D. T. Nguyen, Michael C. Leopold

Chemistry Faculty Publications

Factors affecting the enhanced amperometric signal observed at electrodes modified with polyelectrolyte–gold nanoparticle (Au-NP) composite films, which are potential interfaces for first-generation biosensors, were systematically investigated and optimized for hydrogen peroxide (H2O2) detection. Polyelectrolyte multilayer films embedded with citrate-stabilized gold nanoparticles exhibited high sensitivity toward the oxidation of H2O2. From this Au-NP film assembly, the importance of Au-NP ligand protection, film permeability, the density of Au-NPs within the film, and electronic coupling between Au-NPs (interparticle) and between the film and the electrode (interfacial) were evaluated. Using alternative Au-NPs, including those stabilized with thiols, polymers, and bulky ligands, suggests that the …