Forensic Chemistry Commons^™

Assessing The Impact Dynamics Of Less Lethal Ammunition, Steven Bermejo

Student Theses

Less-lethal ammunition is widely used by law enforcement as an alternative to lethal force during crowd control, on-street confrontations, and other situations requiring force. Although these projectiles are intended to reduce risk of fatal injury, serious injuries and fatalities have been reported, highlighting the need to better understand the factors that influence their impact behavior. This study evaluated the impact dynamics of four types of 12-gauge less-lethal ammunition, including rubber buckshot, single rubber ball, double rubber ball, and Byrna kinetic projectile, using kinetic energy calculations derived from projectile mass and velocity measurements. In addition, Attenuated Total Reflectance Fourier Transform Infrared …

Analysis Of Terpenes In Cannabis Flowers Using Gas Chromatography Via Headspace-Solid Phase Microextraction (Hs-Spme), Hannah I. Bergner

Forensic Science Master's Projects

As cannabis commercialization grows, rigorous profiling of cannabinoids, terpenes, and chemical contaminants is legally required for industry standardization and consumer safety. Terpenes are highly volatile components that dictate the unique aromatic profiles and therapeutic properties of cannabis via receptor pathways. This project focused on a gas chromatography-flame ionization detector/mass selective detector (GC-FID/MSD) combined with headspace-solid phase microextraction (HS-SPME) sampling to analyze terpenes present in four cannabis samples. Cannabis samples were tightly sealed in vials (10 mL) and heated to vaporize terpenes in the headspace above the samples, followed by the extraction of the vaporized terpenes using a polydimethylsiloxane (PDMS)-coated SPME …

Forensic Analysis Of Temporary Tattoo Markers And Inks, Daniel J. G. Chasse Jr

Student Theses

As tattoos rise in popularity, some marker manufacturers have created temporary tattoo markers so that children and adults can experience tattoos without the permanence of a regular tattoo. These new products are marketed explicitly as ‘temporary tattoo markers’ and intended for direct application to the skin. This research aims to scientifically evaluate the inks from these temporary tattoo markers to elucidate their physical and chemical properties using wet chemistry, visual examination, ultraviolet-visible spectroscopy (UV/Vis), Fourier-transform infrared spectroscopy (FT-IR), attenuated total reflectance infrared spectroscopy (ATR-IR), thin layer chromatography (TLC), and diffuse reflectance infrared spectroscopy (DRIFTS) to analyze temporary tattoo markers and …

The Comparative Chemical Analysis Of Colored Henna, Vani D. Bhagwandeen

Student Theses

Henna, a natural dye derived from Lawsonia inermis, is widely used for temporary body art, hair coloring, and cultural decorative practices such as Mehendi. Traditional henna produces a reddish-brown stain through lawsone, a naphthoquinone chromophore that binds to keratin in skin, hair, and nails (Badoni Semwal et al., 2014; de Groot, 2013; Kumar Singh et al., 2015). Commercial henna products have increasingly incorporated synthetic dyes, pigments, and glitter additives to produce a broader range of colors and effects (Blair et al., 2004; Manso et al., 2017; Blackledge & Jones, 2007). Despite their prevalence, limited forensic research has examined the …

Investigating The Detection Ability Of Presumptive Bloodstain Testing Through Concealment Obstacles, Skye E. Lehr

Student Theses

Bloodstain detection can provide valuable information on the ability of presumptive tests. When perpetrators seek to alter the scene of violent crimes to interfere with investigations or flee from justice, crime scene investigation becomes more complex. In this analysis, scenarios where bloodstain evidence is attempted to be removed by household cleaners and covered up by acrylic or oil-based paint, are tested using luminol and Kastle-Meyer direct testing. These bloodstains have been altered by bleach, dish soap or all-purpose cleaner and covered under multiple layers of acrylic or oil-based paint. Evidence is documented both photographically and visually to simulate crime scene …

Characterization Of The Thermally Driven Red Hue Effect In Coated And Uncoated Concrete Systems: Implications For Post-Fire Assessment In Wildland Urban Interface Communities, Juan C. Palominos Jr

Master's Theses

The increasing frequency and intensity of Wildland-Urban Interface (WUI) wildfires, such as the destructive 2025 Pacific Palisades Fire that destroyed over 6,000 structures, have underscored the urgent need for rapid post-fire safety assessment methodologies. This study sought to quantitatively characterize the Thermally Driven Red Hue Effect (TRHE), a unique, irreversible color shift observed in architectural coatings and concrete substrates, to determine its reliability as a permanent visual record of thermal exposure. Concrete substrates were formulated with three distinct coarse aggregates: gold granite (14.7% Fe), red cinder (22.4% Fe), and green rock (33.6% Fe) and coated with a 20 PVC waterborne …

Separating And Identifying Delta-8-Thc And Delta-9-Thc On Gc/Ms, Ann Schmidt

Honors Projects

Delta-8-THC and delta-9-THC are two psychoactive compounds found in marijuana products which are only distinguishable from each other by alternating the location of a single double bond.  This makes them a challenge to separate and identify as different compounds using the GC/MS.  These isomers are legally recognized as the different compounds, so being able to identify delta-8 and delta-9 THC separately is crucial to forensic analysis. The goal of this research project is to optimize an Intuvo 9000 GC connected to a standard MS to be able to separately and prominently identify delta-8-THC and delta-9-THC in a sample.  This was …

Comparison Of Analytical Techniques From The Extraction Of Bioactive Compounds From Kratom, Curry, Ginger, And Turmeric, Riley Bruno

Honors Program: Senior Projects (Public)

Plants, herbs, and spices have been used as medicines for thousands of years. Early civilizations often attributed healing properties of plants to magical or divine forces. However, as chemistry and analytical technology advanced between the 16th and 18th centuries, scientists began to understand that bioactive compounds within the plants actually caused these effects. Today, natural products remain extremely important in drug discovery. As the number of newly developed synthetic drugs declines, there has been renewed interest in identifying biologically active molecules from plants. Bioactive molecules are a group of diverse chemical compounds that stimulate a response in living tissues. The …

Lc-Ms/Ms Method Development For The Analysis Of Cannabinoids In Wastewater, Abigail M. Castellanos, Ava La Lande

The Kean Review: Journal in Student Research

The goal of this research was to establish a reliable method to detect the presence of cannabinoids in wastewater and increase the sensitivity of detection. Since the legalization of cannabis in many states, toxicology research has expanded on methods of detection in hopes of identifying usage patterns. At the Joint Meeting of Essex and Union Counties, it was established that cannabinoids are not thoroughly accounted for in the process of filtering wastewater in addition to our limited understanding of consumption trends. This stands true for many wastewater facilities, especially as the intake of cannabis remains prevalent for various populations across …

Development And Validation Of An Analytical Method For Quantitative Determination Of Small Aldehydes In Plasma, Stephanie T. Clark

Chemistry & Biochemistry Theses - Archive

Small aldehydes such as formaldehyde and acetaldehyde are highly reactive metabolites that play important roles in biological processes related to oxidative stress, metabolism, and DNA damage. Because of their chemical reactivity, volatility, and low endogenous concentrations, accurate measurement of these compounds in biological samples remains analytically challenging. The goal was to develop and evaluate a practical analytical method for detecting small aldehydes in biologically relevant matrices using headspace gas chromatography mass spectrometry (HS-GC-MS). The method combined chemical derivatization with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (PFBHA) and headspace sampling to stabilize aldehydes and enable selective detection by mass spectrometry. Method development focused on optimizing derivatization …

The Forensic Implications Of Hydrochloric Acid (Hcl) Exposure On Dna Preservation In Human Teeth, Shaelyn Lee Zimmerman

Graduate Student Theses, Dissertations, & Professional Papers

Identity is a human right, and forensic anthropological methods are often aimed at returning identity to decedents. Three main pathways are used for the identification of human remains: fingerprints, dental records, and DNA analysis. Identification efforts may be hindered when perpetrators attempt to obscure the victim’s identity. In cases where corrosive substances, such as hydrochloric acid (HCl) are used, dental comparison and fingerprinting often fail and genetic analysis becomes the best chance of achieving personal identification.

Previous studies have shown teeth are an excellent source of DNA. Large, multi-rooted teeth, such as molars, are often preferred because they contain the …

The Deposition, Prevalence, And Persistence Of Gunshot Residue In Standard And Less Lethal Ammunition, Liliana Claire Barbosa

Graduate Theses, Dissertations, and Problem Reports (ETD)

During the discharge of a firearm, gunshot residue (GSR) is released and settles into nearby surfaces, leaving traces that can provide valuable information for forensic investigations. This study aims to improve understanding of the deposition, prevalence, and persistence of standard and less-lethal ammunition through an analytical framework that examines collection and analysis protocols, revealing connections among multiple traces for effective use in investigations and courtroom proceedings.

Research into the chemical residues of less lethal ammunition (LLA) is a critical frontier in shooting investigations. While standard leaded ammunition has over a century of established literature, the chemical fingerprints of LLA residues …

The Chemistry Of Red Lipstick: The Impact Red Lipstick Has On Society, Lilliana Weldeslassie

Electronic Theses & Dissertations (2024 - present)

Cosmetic chemistry is deeply embedded in everyday life, shaping the products people use from morning to night. Among these, red lipstick stands out as a formulation that blends complex chemistry with a cultural meaning. This thesis examines the scientific foundations of red lipstick through its chemical composition, pigment structure, toxicological history, analytical methods, and evolving sustainability practices. Historically, red lipsticks relied on high-risk ingredients such as lead, mercury, and other toxic metals, reflecting an era before chemical safety and regulatory oversight were incorporated.²¹ Modern formulations have established safer synthetic dyes, natural waxes, plant-based oils, and stabilizers created to optimize …

Evaluating The Comparison Of Different Body Locations For Data Collection Using Galvanic Skin Response Of Forensic Chemistry Students When In Lecture And Laboratory Environments, Elisa Guerrero

Honors Undergraduate Theses

Anxiety is the response that humans can have when exposed to a stimulus. It can be caused by a multitude of factors, such as psychological, biological, or physiological stimuli. Anxiety can be presented as a state or as a trait in a person. Trait anxiety is typically seen as a more stable personality trait, whereas state anxiety is a more intense temporary emotional state. In our study, anxiety was regarded as a more state-like characteristic. A galvanic skin response (GSR) Shimmer3+ device can measure skin electro-conductance changes following stimulation. In a situation where a participant is experiencing anxiety, levels of …

Balancing Evidence Recovery: Hexane’S Effect On Biological And Non-Biological Traces In Sexual Assault Kits, Maya Richards

Honors Undergraduate Theses

This study investigated the effectiveness of solvent – based extraction methods for the recovery of lubricant residues from simulated sexual assault swabs, with emphasis on balancing chemical evidence recovery. Sequential extraction using ultra – pure water followed by organic solvents, hexane, dichloromethane (DCM), and ethyl acetate (EA), was evaluated across water – based, silicone – based, oil – based, and hygiene product matrices. Analysis was performed using Direct Analysis in Real Time – High Resolution Mass Spectrometry (DART – HRMS), and extraction efficiency was assessed through spectral correlation with neat reference samples. Results demonstrated that solvent performance was highly dependent …

Quantification Of Terpenes In Various Commercial Cannabis Products By Gas Chromatography-Flame Ionization Detection And Gas Chromatography-Mass Spectrometry, Kaitlyn M. Ignaszak

Forensic Science Master's Projects

The goal of this research project was to identify and quantify the terpenes in five commercial cannabis products (dried bud, kief, two tinctures, and a pain cream) using gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Terpene identification and quantification were conducted using GC-MS and GC-FID, with a standard sample containing 21 terpenes of known concentrations. To determine the most effective extraction conditions, three organic solvents (ethanol, acetonitrile, and ethyl acetate) were employed. Additionally, the effects of extraction time on terpene yield were investigated by analyzing aliquots collected at five different time intervals, ranging from one hour to …

Highly Contaminated Sediments From The Gowanus Canal (New York) Superfund Site: An Environmental Forensic Approach, Michael A. Kruge, Kevin K. Olsen, Eric A Stern, Maria Mastalerz, Albert Permanyer

Department of Earth and Environmental Studies Faculty Scholarship and Creative Works

The 2.5 km long Gowanus Canal (Brooklyn, NY, USA) is a severely contaminated urban waterway dating from the mid-19th century, listed as a US Environmental Protection Agency (USEPA) Superfund site since 2010. Applying an environmental forensics approach to the extensive USEPA data set, we detect systematic variations in parent PAH ring number distributions in the canal sediments, as well as an extraordinary enrichment in organic carbon. We subjected a supplemental sample set to a more detailed analysis by quantitative pyrolysis-GC-MS, Rock-Eval pyrolysis, and organic petrology. With these sensitive methods, we can confirm the alkyl-PAH fingerprint of coal tar (a legacy …

Color Tests For The Presumptive Identification Of A Range Of Benzodiazepines, Regan Mulvin

Honors Projects

Color tests are presumptive chemical tests that determine the class of drug in both laboratory and field settings. Despite the rising popularity of benzodiazepines and the usefulness of color tests, there is no widely accepted color test for identifying them. This study tested six different color tests against five benzodiazepines, including diazepam, clonazepam, lorazepam, alprazolam, and flualprazolam, to identify which of the tests would work most generally across all benzodiazepines. Of the six tests, the McKibben test, the Janovsky test, and the Zimmerman test all showed potential usefulness as a presumptive color test. More studies should be done on a …

The Chemistry Of Decomposition: The Mechanisms To Decay The Human Body, Lydia J. Buxa

Senior Honors Theses

Taphonomic analysis separates decomposition into four major stages: fresh, early decomposition, advanced decomposition, and skeletonization. It is easy to demarcate the observable changes to the body, but it is far more difficult to know what underlying biochemical processes are occurring. Fresh decomposition begins with algor mortis, livor mortis, and rigor mortis, which sets the stage for the major workforces of decomposition: autolysis and putrefaction. The external signs of decomposition appear in early decomposition, which can be attributed to the breakdown of proteins, lipids, carbohydrates, and nucleic acids. This also leads to major changes in the moisture levels during advanced decomposition, …

Identification Of Areas Of Weakness In Forensic Footwear Research, Kathryn Roy

Honors Projects

Forensic footwear examinations have been carried out for almost a century, but the foundational validity of identifying a questioned impression to a particular shoe has been questioned by the National Research Council and the President’s Council of Advisors on Science and Technology. The focus of this paper was to identify the current weaknesses present in forensic footwear impression research. Additionally, a future experiment was proposed to address these concerns and improve the foundational validity of footwear impression comparisons. A total of 15 footwear research studies varying in methodology and content were analyzed and contrasted. From this, a lack of studies …

The Analysis Of Reference Standard Storage Failures Through The Use Of Ibuprofen, Hope Vonder Brink

Honors Projects

Reference standards are commonly used in drug chemistry. They are used to calibrate the instruments and serve as a known point of reference for analysts’ ability to compare unknowns. Drugs can degrade over time or through environmental factors, causing contaminants to be present. In the event of environmental degradation while in storage, such as the exposure to high heat, it would be beneficial to have a database of known breakdown products in common drug reference standards for analysts’ reference. Using ibuprofen as an exemplar drug, this study attempted to identify the thermal breakdown products of ibuprofen and introduce the proposed …

Unraveling The Reactivity Of Nitrate Ester Explosives: Insights From Femtosecond Time-Resolved Mass Spectrometry And Computational Chemistry, Erica Britt

Theses and Dissertations

This dissertation uncovers the ultrafast molecular mechanisms underlying the decomposition of nitrate ester explosives—nitroglycerin (NG), ethylene glycol dinitrate (EGDN), and amyl nitrate—through the integration of femtosecond time-resolved mass spectrometry and advanced computational chemistry. Although these compounds play fundamental roles in both military and civilian contexts, the precise molecular dynamics governing their pronounced reactivity and impact sensitivity have remained inadequately characterized. Our research establishes that low O–NO2 bond dissociation energies are the primary driver of ultrafast fragmentation and high reactivity in all three esters. The distinctive molecular architectures of these esters dictate their explosive properties: NG’s tri-nitrate configuration leads to exceptional …

Assessment Of Analytical Workflows Of Gunshot Residue Evidence And Strategies For Implementation Of Advanced Technology In Crime Laboratories., Leah D. Thomas

Graduate Theses, Dissertations, and Problem Reports (ETD)

The rise in gun violence in the United States has increased the demand on forensic agencies to process evidence promptly and accurately. The exchange of imperceptible traces among objects, suspects, and victims can provide critical insights into the interactions and sequence of events during a crime. Gunshot residue (GSR) is a type of trace evidence that holds significant investigative value in shooting reconstructions. GSR can help identify potential shooters, intermediate targets, and bullet trajectories; however, its analysis is often expensive and time-consuming. Consequently, there is an urgent need for more effective solutions.

Methods for on-site screening can significantly streamline forensic …

Understanding The Prevalence Of Γ-Hydroxybutyric Acid In Hair For Clinical And Forensic Applications, Collin J. Kustera

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sexual assault affects over 400,000 Americans annually, including 13% of college students, yet only 2.5% of assailants face incarceration. This disparity stems from significant challenges in evidence collection, particularly in drug-facilitated crimes (DFCs). Victims often delay reporting due to fear, self-blame, or unawareness of the assault which is common in cases involving substances like γ-hydroxybutyric acid (GHB). Current forensic standards rely on blood and urine, which offer detection windows of just 48 and 120 hours, respectively. These short timeframes frequently fail to capture critical toxicological evidence, leaving investigators with only victim testimony, which the judicial system struggles to act upon …

Refinement Of A Thermodynamic Model To Explain The Weathering Patterns Of Ignitable Liquids On Household Substrates At Elevated Temperatures, Chaney Ashlyn Ganninger

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Refinement of a thermodynamic model to explain the weathering patterns of ignitable liquids on household substrates at elevated temperatures

Chaney A. Ganninger

In 2021, arson caused more than 4,361 deaths in the United States. To aid in the differentiation between arson and accidental or natural causes, investigators often analyze fire debris for the presence of ignitable liquid residues using headspace concentration followed by gas chromatography-mass spectrometry (GC-MS). Classification of ignitable liquids can be confounded by weathering, which is the uneven evaporation of compounds in ignitable liquid residues. The Jackson group, among others, has developed a thermodynamic model to help …

High Throughput, Solid Sample Analysis With Cone-Based Mass Spectrometric Ionization Sources, Emily J. Wiggins

Theses and Dissertations

The widespread presence of environmental and consumer-product contaminants (e.g. trace metals, opium alkaloids) has underscored the need for rapid, reliable, and high-throughput analytical techniques that are capable of handling complex solid samples. While traditional mass spectrometric (MS) methods tend to have high sensitivity and accuracy, they often require labor-intensive and time-consuming sample preparation steps that limit throughput and field applicability. This thesis investigates the use of 3D-Printed Cone Spray Ionization-Mass Spectrometry (3D-PCSI-MS) as an ambient ionization technique for the direct screening of contaminants from solid matrices, eliminating the need for traditional sample preparation and allowing for real-time analysis.

In …

Forensic Characterization And Comparison Of Modern Nail Polish Products By Their Physical Features And Chemical Composition, Madison Meredith Lindung

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nail polish can serve as valuable evidence in forensic investigations as it is often found on the hands and feet of individuals. Nail polish can break off and transfer easily during self-defense actions or forceful contact, such as in cases of kidnapping, sexual assault, or homicide. These residues can provide clues about who was at the scene, as well as where, when, and how the event evolved. However, compared to other types of paints commonly analyzed in forensic laboratories, limited forensic research has been conducted on the chemical composition and discrimination capabilities of popular nail products. The lack of information …

Identification Of Fentanyl And Fentanyl Analogs Found In Human Urine Using Gas Chromatography-Mass Spectrometry, Adam M. Fisher

Electronic Theses & Dissertations (2024 - present)

Identification of fentanyl and its analogs in unknown samples is a frequent practice completed in forensic crime labs, as the fentanyl epidemic continues. With the continued increase in illicit drug use, crime labs are not able to keep up with the flooding of new fentanyl analogs being developed, resulting in new analogs slipping past the crime labs’ authority. This research is to start to develop a database of commonly found fentanyl analogs in New York State. Gas Chromatography-Mass Spectrometry (GC-MS) is a widely used method for the detection and identification of illicit drugs in forensic crime labs. A database that …

Developing Consensus-Based Methods For The Examination And Interpretation Of Contemporary Vehicle, Architectural, And Portable Electronic Device Glasses By Micro-X-Ray Fluorescence Spectrometry, Zachary Bailey Andrews

Graduate Theses, Dissertations, and Problem Reports (ETD)

Glass is a trace material that is commonly encountered during investigations of violent crimes. When glass is recovered at crime scenes, it can be used to establish links between suspects, victims, and the scene itself. The most discriminatory form of analysis for glass evidence is elemental analysis, and micro-X-ray fluorescence spectrometry (µXRF) is becoming an increasingly common technique used for this purpose. Recent advances in µXRF technology, such as the introduction of silicon drift detectors (SDD) and improved polycapillary optics are promising in enhancing the capabilities for the examination of glass evidence in forensic investigations. However, along with these advances …