Chemistry Commons^™

Synthetic Flexibility Of A Bromopyrrole Ester Intermediate : Toward Novel Biologically Active Compounds, Scott Cameron Yeudall

Honors Theses

Compounds containing the pyrrole ring as a core structural motif continue to show significant biological activity, and both natural product derivatives and novel chemical scaffolds show potential for use as pharmaceuticals in treating a variety of cancers, infections, and inflammation. Given the widespread and important applications of compounds containing this motif, novel, rapid, and selective methods for the synthesis of multifunctional pyrroles is of some importance. Previous work in our group has utilized palladium-mediated Suzuki cross-coupling as a powerful tool for functionalizing activated bromopyrrole esters, generating structural analogues of bioactive natural products. We have also used a similar approach to …

Structure-Function Relationships Affecting The Sensing Mechanism Of Monolayer-Protected Cluster Doped Xerogel Amperometric Glucose Biosensors, Luke T. Dipasquale

Honors Theses

The research conducted in the Leopold Bioanalytical and Nanomaterials Analytical Chemistry Lab prior to the summer of 2013 focused on the synthesis of a functional biosensor equipped with a nanoparticle network embedded in a xerogel film for the detection of glucose. Xerogel-based films featuring embedded glucose oxidase enzyme and doped with alkanethiolate-protected gold NPs, known as monolayer protected clusters (MPCs), exhibit significantly enhanced performance compared to analogous systems without NPs including higher sensitivity, faster response time, and extended linear/dynamic ranges.

The following presented research is a systematic study of the structure-function relationships critical to understanding the sensing mechanism of the …

Tracking Silver, Gold, And Titanium Dioxide Nanoparticles Through Drinking Water Systems By Single Particle - Inductively Coupled Plasma - Mass Spectrometry, Ariel Renee Donovan

Masters Theses

"Single particle (SP)-ICP-MS methods were developed to characterize and quantify Ti-containing, titanium dioxide, silver, and gold NP concentration, size, size distribution and dissolved metal element concentration in surface water and treated drinking water. The effectiveness of conventional drinking water treatments (including lime softening, alum coagulation, filtration, and disinfection) to remove NPs from surface water was evaluated using six-gang stirrer jar test simulations. Six-gang stirrers were used to simulate drinking water treatments including lime softening, alum coagulation, powdered activated carbon sorption, filtration, and disinfection by free chlorine. Lime softening effectively removed most nanoparticles added. Source and drinking waters from three large …

Characterization And Photocatalytic Properties Of Tio$_{2}$/Chitosan Nanocomposites Synthesized By Hydrothermal Process, Funda Okuşluk, Fatma Bi̇lge Emre

Turkish Journal of Chemistry

Nanocomposites of TiO$_{2}$/chitosan (nTC) were synthesized by hydrothermal process at 200 $^{\circ}$C. Photocatalytic activity of the nanocomposites was studied with degradation of malachite green (MG) in aqueous medium. Structural and physico-chemical properties of the obtained nanocomposites were characterized by XRD, SEM, BET, FTIR, and particle size analyzer. Complete photodegradation of MG was successfully achieved with the aid of the nanocomposites. The optimum photocatalytic degradation conditions, irradiation time, irradiation power, and amount of catalyst were studied. Photocatalytic activity of the synthesized nanocomposites in terms of degradation of MG was compared with that of undoped TiO$_{2}$ (synthesized by our team) and chitosan …

Systematic Optimization Of Chemical Deposition Conditions For Synthesis Of Vanadium(V) Oxide Xerogels, Aksu Samet, Metodija Najdoski, Igor Kuzmanovski

Turkish Journal of Chemistry

We performed the optimization of conditions in chemical bath deposition using the design of experimental methodology. Factorial design (2$^{3})$ was used for finding the optimal conditions of preparing vanadium(V) oxide xerogel thin films with better electrochromic properties suitable for electrochemical applications. The thin films were prepared by acidification of NH$_{4}$VO$_{3}$ aqueous solution with chemical bath deposition. The factors and selected values we considered were as follows: the deposition time (10 and 20 min), the deposition temperature (70 and 80 $^{\circ}$C), and the mass of ammonium metavanadate (0.3 and 0.5 g). UV-vis spectrometry was used for recording the transmittance variance, $\Delta …

Synthetic Approaches Towards The Synthesis Of Beta-Blockers (Betaxolol, Metoprolol, Sotalol, And Timolol), Furqan Ahmad Saddique, Ameer Fawad Zahoor, Muhammmad Yousaf, Muhammmad Irfan, Matloob Ahmad, Asim Mansha, Zulfiqar Ali Khan, Syed Ali Raza Naqvi

Turkish Journal of Chemistry

Numerous efficient synthetic methodologies have been elaborated for the synthesis of $\beta $-blockers since the introduction of propranolol (a beta-blocker) in 1968. In this review, focus is placed on the more concise asymmetric and bioenzymatic synthetic approaches attempted towards the synthesis of beta-blockers (betaxolol, metoprolol, sotalol, and timolol).

Synthetic Access To Some New Benzothiazole-Based 1,3,4-Thiadiazole And 1,3-Thiazole Derivatives, Kamal M. Dawood, Eman A. Ragab, Korany A. Ali

Turkish Journal of Chemistry

1-(Benzothiazol-2-yl)-3-phenylthiourea\textbf{ 2} was prepared and treated with hydrazonoyl chlorides \textbf{3a}--\textbf{e }to yield the corresponding 5-(benzothiazol-2-ylimino)-1,3,4-thiadiazole derivatives \textbf{6a}--\textbf{e}, respectively. Reaction of the thiourea derivative \textbf{2} with ethyl 2-chloro-3-oxobutanoate \textbf{9} afforded the corresponding 2-(benzothiazol-2-ylimino)thiazole-5-carboxylate derivative \textbf{11}. The newly synthesized heterocyclic derivatives were confirmed from their elemental and spectral analyses.

Synthesis And Evaluation Of Some Novel Thiazoles And 1,3-Thiazines As Potent Agents Against The Rabies Virus, Magda Abdalla, Sobhi Gomha, Mohamad Abd Elaziz, Nany Serag

Turkish Journal of Chemistry

A series of novel thiazoles and 1,3-thiazine derivatives were synthesized in good yield via reaction of ethyl 3-(1-(2-thiocarbamoylhydrazono)ethyl)-1,5-diphenyl-1$H$-pyrazole-4-carboxylate with hydrazonoyl halides and arylidenemalononitriles, respectively. The structure of the newly synthesized products was elucidated via elemental analysis, spectral data, and alternative routes whenever possible. Moreover, the antiviral screening of the products was evaluated and the results revealed that some of them have strong to moderate potency against the rabies virus compared with the reference drug.

Prostereoisomerism And Biological Activity: Possible Implications For Drug Design, Sosale Chandrasekhar

Turkish Journal of Chemistry

Prostereoisomerism (PIM), representing a subgroup within the achiral class of molecules, is manifested in a chiral environment. Although this is regardless of the orientation of the prostereoisomeric (PIC) molecule, PIM is manifested most emphatically in relation to a chiral surface (`two-dimensional chirality'). Then PIM is tantamount to `de facto chirality' as attachment of the PIC molecule to the surface leads to diastereomeric possibilities (cf. Ogston's hypothesis). Furthermore, the formation of host--guest complexes requires a steric complementarity between the component molecules. The fact that the weak dispersive forces involved therein require an optimum distance implies a `snugness of fit' criterion. This …

Preparation, Characterization, And Adsorption Studies Of Core@Shell Sio$_{2}$@Ceo$_{2}$ Nanoparticles: A New Candidate To Remove Hg(Ii) From Aqueous Solutions, Ali̇ İmran Vai̇zoğullar, Ahmet Balci, İbrahi̇m Kula, Mehmet Uğurlu

Turkish Journal of Chemistry

SiO$_{2}$ supported core@shell nanoparticles (CSNs) have recently attracted great attention due to their unique, tunable, optical, photocatalytic, and higher adsorption properties. In this study, SiO$_{2}$@CeO$_{2}$~CSNs were synthesized using a chemical precipitation technique and characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscope (SEM), and transmission electron microscope (TEM) analysis. XRD analysis showed that SiO$_{2\, }$particles were the core while CeO$_{2}$ particles were the shell. It was seen as a new band at 961 cm$^{-1}$ of the oxygen bridge between Si and Ce atoms from FT-IR results; SiO$_{2}$ and CSNs were spherical (0.5--0.6 $\mu $m) from SEM and …

Experimental And Theoretical Investigation Of The Reaction Between Co$_{2}$ And Carbon Dioxide Binding Organic Liquids, Hi̇lal Tankal, Özge Yüksel Orhan, Erdoğan Alper, Telhat Özdoğan, Hakan Kayi

Turkish Journal of Chemistry

The reaction kinetics of CO$_{2}$ absorption into new carbon dioxide binding organic liquids (CO$_{2}$BOLs) was comprehensively studied to evaluate their potential for CO$_{2}$ removal. A stopped-flow apparatus with conductivity detection was used to determine the CO$_{2}$ absorption kinetics of novel CO$_{2}$BOLs composed of DBN (1,5-diazabicyclo[4.3.0]non-5-ene)/1-propanol and TBD (1,5,7-triazabicyclo[4.4.0]dec-5-ene)/1-butanol. A modified termolecular reaction mechanism for the reaction of CO$_{2}$ with CO$_{2}$BOLs was used to calculate the observed pseudo-first-order rate constant k$_{0}$ (s$^{-1})$ and second-order reaction rate constant k$_{2}$ (m$^{3}$/kmol.s). Experiments were performed by varying organic base (DBN or TBD) weight percentage in alcohol medium for a temperature range of 288-308 K. …

Zr(Iv) Complexes Of Some Heterocyclic Ligands: Synthesis, Characterization, And Ethylene Polymerization Activity, Hamdi Ali Elagab

Turkish Journal of Chemistry

Thirty-one complexes of bis-(benzimidazole, benzothiazole, and benzoxazole) compounds with Zr(IV) metal centers were synthesized, characterized, activated with methylaluminoxane (MAO), and then tested for catalytic ethylene polymerization. The activities of the various catalysts were found to be functions of the heteroatoms in the ligand frameworks and the structure around the active metal center. The highest activity was obtained with 38/MAO (424 kg E/mol cat. h). The produced polyethylenes showed high molecular weights (41/MAO, 1.9 $\times$ 10$^{6}$ g/mol) and broad molecular weight distributions (38/MAO, M$_{w}$ = 9.64 $\times $ 10$^{5}$ g/mol, PD = 23). This could result from different interactions of the …

Speciation Of Inorganic Selenium By Solid Phase Extraction Using Nanozirconium Oxide/Boron Oxide Composite Material, Hakan Erdoğan, Özcan Yalçinkaya, Ali̇ Rehber Türker

Turkish Journal of Chemistry

The speciation and preconcentration of selenium were carried out by solid phase extraction using nanozirconium oxide/boron oxide (ZrO$_{2}$/B$_{2}$O$_{3})$. Electrothermal atomic absorption spectrometry (ETAAS) was used for detection. This preconcentration and/or speciation method depends on the retention of Se(IV) ions selectively as its ammonium pyrrolidine dithiocarbamate (APDC) complex on the sorbent. The effects of pH, flow rate, and volume of sample solution and eluent type were investigated for determining the optimum preconcentration and speciation conditions. The effects of other ions that may interfere with the determination and speciation of selenium were also investigated. Moreover, the analytical performance criteria of the method …

Degradation Of Bisphenol-A Using A Sonophoto Fenton-Like Hybrid Process Over A Lafeo$_{3}$ Perovskite Catalyst And A Comparison Of Its Activity With That Of A Tio$_{2}$ Photocatalyst, Meral Dükkanci

Turkish Journal of Chemistry

Oxidation of bisphenol-A (BPA) was investigated using a sonophoto Fenton-like hybrid process under visible light irradiation in the presence of iron-containing perovskite LaFeO$_{3}$ catalysts. For this purpose, firstly the perovskite catalyst (LaFeO$_{3})$ was prepared by the sol-gel method and calcined at different temperatures (500, 700, and 800 $^{\circ}$C). The prepared catalysts were characterized using XRD, SEM, FTIR, nitrogen adsorption, UV-vis DRS, and ICP/OES measurements. Among the prepared catalysts the catalyst that was calcined at 500 $^{\circ}$C showed better catalytic activity with respect to degradation and chemical oxygen demand (COD) reduction (of 21.8% and 11.2%, respectively, after 3 h of reaction …

Synthesis, ($E)$/($Z)$-Isomerization, And Dna Binding, Antibacterial, And Antifungal Activities Of Novel Oximes And $O$-Substituted Oxime Ethers, Hati̇ce Başpinar Küçük, Ayşe Sergüzel Yusufoğlu, Leyla Açik, Betül Aydin, Leyla Arslan

Turkish Journal of Chemistry

A series of novel positional oximes (2a-2d), $O$-methyl oxime ethers (3a-3d), and $O$-benzyl oxime ethers (4a-4d) were synthesized in high yields starting from their corresponding methyl 3-, 4-, 6-, and 13-keto tetradecanoates. The synthesized compounds were characterized by $^{1}$H NMR, $^{13}$C NMR, FT-IR, mass, and elemental analyses for their structures and $(E)$/$(Z)$ isomerizations. Their DNA binding abilities were investigated in vitro by agarose gel electrophoresis. The antibacterial and antifungal activities were tested also in vitro against eleven bacterial strains and three fungal strains. The relationship between the structure and the mentioned biological activities was discussed. Compound 2a showed good antibacterial …

Speciation Of Trace Metals And Metalloids By Solid Phase Extraction With Spectrometric Detection: A Critical Review, Ali̇ Rehber Türker

Turkish Journal of Chemistry

Considerable interest is continuing to be shown in the speciation of metals, metalloids, and organometals due to the dependence of their toxicity and mobility on their chemical form. This review focuses on the speciation analysis of metals, metalloids, and organometals by solid phase extraction before spectrometric detection, and all aspects of analytical speciation such as determination of oxidation states and organometallic compounds. Some applications of speciation of metals and metalloids are also presented and discussed in this review. Fractionation, which is used sometimes as speciation, and chromatographic detection are not discussed. This review covers approximately the last four years (2012-March …

Spectrophotometric Determination Of Mercury Using Vortex-Assisted Liquid-Liquid Microextraction, Tatiana Antolova, Serhii Zaruba, Jana Sandrejova, Livia Kocurova, Andriy B. Vishnikin, Yaroslav Bazel, Vasil Andruch, Ioseph S. Balogh

Turkish Journal of Chemistry

A novel method for the determination of mercury(II) is suggested. The procedure is based on the formation of an ion associate between the bromide complex of Hg(II) and Astrazon Red 6B dye and vortex-assisted liquid-liquid microextraction of the ion associate formed, with subsequent spectrophotometric detection. The variables that affect the procedure, such as pH, the concentration of ligand and dye, the type and volume of extraction solvent, and the rate and time of vortex mixing, were optimized. Under optimum conditions (pH 2.0, 0.01 mol L$^{-1}$ KBr, 2 $\times $ 10$^{-4}$ mol L$^{-1}$ AR6B, 50 $\mu $L of the extraction mixture …

An Application Of Coacervate-Based Extraction For The Separation And Preconcentration Of Cadmium, Lead, And Nickel Ions Prior To Their Determination By Flame Atomic Absorption Spectrometry In Various Water Samples, Anies Awad Satti, İlknur Durukan Temuge, Sema Bektaş, Çi̇ğdem Arpa Şahi̇n

Turkish Journal of Chemistry

Cloud point extraction (CPE), which is the most popular coacervate-based extraction method, was utilized for the separation and preconcentration of trace amounts of cadmium, lead, and nickel. Quinalizarin was used as complexing agent and Triton X-114 was used as surfactant. After extraction, analyte ions were determined by flame atomic absorption spectrometry. The detection limits (3$s)$ of 1.8 $\mu $g L$^{-1}$ for Cd(II), 3.2 $\mu $g L$^{-1}$ for Pb(II), and 2.8 $\mu $g L$^{-1}$ for Ni(II) were obtained using 50 mL of solutions. The relative standard deviation was calculated as 4.3% for 10 $\mu $g L$^{-1}$ Cd(II), 4.9% for 50 $\mu …

Separation And Preconcentration Of Pb(Ii) And Cu(Ii) Ions Via Carrier Element-Free Coprecipitation Using An Acetohydrazide Derivative, Süreyya Oğuz Tümay, Murat Yazar, Duygu Özdeş, Hakan Bektaş, Celal Duran

Turkish Journal of Chemistry

A new method based on the combination of carrier element-free coprecipitation and flame atomic absorption spectrometric determination has been investigated for simultaneous separation and preconcentration of Pb(II) and Cu(II) ions in some environmental solid and liquid samples. The 2-{3-(4-methylbenzyl)-4-[2-(1H-indol-3-yl)ethyl]-5-oxo-4,5-dihydro-1H-1,2,4-triazol-1-yl} -N'-(phenylmethylene) acetohydrazide (MITA) was utilized as an organic coprecipitating agent without using a carrier element for coprecipitation of the analyte ions. The effects of experimental conditions including aqueous solution pH, MITA quantity, sample volume, standing time, and centrifugation rate and time were investigated in detail and optimized. The influences of some foreign ions were also evaluated on the quantitative recoveries of …

Anodic Metal Dissolution In Haloaluminate Molten Salts/Ionic Liquids, Chen Wang

Electronic Theses and Dissertations

The anodization of Aluminium was investigated in the low-melting, mixed halide molten salt system, lialbr4-naalcl4-kalcl4 (30-50-20 m/o), and in the Lewis acidic Chloroaluminate ionic liquid, aluminum chloride-1-ethyl-3-methylimidazolium chloride (alcl3-etmeimcl) using rotating disk electrode voltammetry. In both cases, at modest overpotentials, the anodization reaction proceeds under mixed kinetic/mass-transport control. However, at larger positive overpotentials and correspondingly higher anodization rates, the reaction transitions to a mass transport-limited process governed by the dissolution of a passive layer of alcl3(s) and/or albr3(s) on the electrode surface. In alcl3-etmeimcl, the passive current density scales linearly with the concentration of alcl4- in the ionic liquid. The …

Removal Of Endocrine Disrupting Compounds From Water And Wastewater Using Ecofriendly Materials, Alma Lili Loya Posada

Open Access Theses & Dissertations

Endocrine disrupting chemicals (EDCs) are compounds considered pollutants of emerging concern. The United States Environmental Protection Agency (USEPA) defines emerging pollutants as new chemicals without regulatory status and with an impact on the environment and human health that is poorly understood. Bisphenol A (BPA) has been considered an EDC and found to be ubiquitous in the environment because of its presence in a multitude of products including food and beverage packaging, flame retardants, adhesives, building materials, electronic components, and paper coatings Currently, BPA is not regulated by the federal government in the US and waste water treatment plants are not …

Synthesis Of Neoglycoproteins/Neoglycopeptides And Their Immunological Evaluation In The Context Of Chagas Disease, Nathaniel Scott Schocker

Open Access Theses & Dissertations

The protozoan parasite, Trypanosoma cruzi, the etiologic agent of Chagas disease (ChD), has a cell surface covered by immunogenic glycoconjugates. A synthetic glycoarray containing non-reducing α-galactopyranosyl moieties related to mucin O-glycans of the parasite T. cruzi was evaluated by a chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) with sera from patients with chronic ChD, revealing the disaccharide Galα(1,3)Galβ as the immunodominant glycotope (Chapter 2). This disaccharide comprises the two terminal sugars of the known immunodominant glycotope Galα(1,3)Galβ(1,4)GlcNAcα, which is expressed on glycosylphosphatidylinositol-anchored mucins of the infective trypomastigote stage of T. cruzi and triggers high levels of protective anti-α-Gal antibodies (Abs) in infected …

Conformational Analysis Of A Furan, Thiophene Alternating Π System, Laura Cline

Honors Theses

Organic materials with conjugated Ï€-electron networks are increasingly being studied as an alternative to inorganic semiconductors. In order to effectively transport charge as a semiconductor, the organic materials should maintain a planar conformation, exhibit Ï€ conjugation, and be rigid to rotational interconversion. Both furan and thiophene systems have been investigated as potential organic semiconductors but difficulties with solubility, synthesis, and effectiveness hampered these efforts. A novel furan-thiophene hybrid system that exhibits promising semiconducting properties is examined here. Full geometry optimizations and corresponding harmonic vibrational frequency computations were run on a diverse set of potential furan and thiophene oligomer structures using …

The Synthesis Of Substituted Pentadentate Ligand Scaffolds And Their Future Application In Ruthenium-Catalyzed Water Oxidation, Manpreet Singh

Honors Theses

Rising global energy demands coupled with depleting natural resources have led to a strong emphasis on finding new sources of energy that are renewable and which limit the negative impact on the environment. One of the strongest candidates is utilizing solar energy to drive water oxidation to produce hydrogen as a fuel source. In order to do this, a better understanding of the mechanism involved in this process must be revealed. Homogenous molecular catalysts allow mechanistic studies and can be also used in Photoelectrochemical Cells (PECs) and other systems to produce chemical fuels. In this work, the new syntheses of …

The Evolution Of The Sugarcoated American Food Market And The Paradox Of Artificial Sweeteners, Mary Moses Hitt

Honors Theses

Living in present-day America makes it hard to imagine an era when sugar was not as inexpensive and readily available as it is now. It is in little colorful packets on restaurant tables, filled to the brim in jars at coffee shops, or mixed into candy products located at the beginning of every cash register. Today, sugar is one of, if not, the most widely consumed food products in the world, but our love for sugar is nothing new. Humans have been experiencing the bliss triggered by sweet-tasting foods since their earliest diets, which included fruits and honey. Our diets …

Synthesis Of Dmap Tethered Polymer Chains For Reversible Micelle Formation, Richard Arthur Fredricks

Master's Theses and Doctoral Dissertations

Dimethylaminopyridine (DMAP) can act to catalyze numerous organic reactions. To increase its recovery and reuse, it has been tethered to dendrimers and to micellar systems, creating nano-scale catalytic pockets for reactions to proceed. Previously developed systems show diminished reactivity over time as the products of the reaction fill up the nano-scale reactor. Thus, the goal of my research is to use RAFT polymerization methods to synthesize a hydrophobic polymer chain bearing 4-methylaminopyridine units and to attach it to a hydrophilic polymer chain to create a polymer system capable of micelle formation in solution. These functionalized micelles are designed to be …

The Nobel Prize In Chemistry 2015: Exciting Discoveries In Dna Repair By Aziz Sancar, David K. Orren

Toxicology and Cancer Biology Faculty Publications

On October 7, 2015, the Nobel Prize in Chemistry for 2015 was awarded to three deserving scientists for their pioneering research on DNA repair. Tomas Lindahl was recognized for studies that uncovered the inherent instability of DNA as well as the mechanism of the base excision repair pathway, Paul Modrich for characterization of the mismatch repair pathway, and Aziz Sancar for mechanistic elucidation of the nucleotide excision repair (NER) pathway. For me, the announcement of these awards in my area of research was extremely gratifying, particularly so because Aziz was my mentor during my Ph.D. studies that examined the steps …

Synthesis And Characterization Of Functionalized Poly(Arylene Ether Sulfone)S Using Click Chemistry, Kavitha Neithikunta

Browse all Theses and Dissertations

Poly(arylene ether sulfone)s with pendent aryl iodide group was successfully synthesized via Nucleophilic aromatic polycondensation of 1-(3,5-difluorophenyl sulfonyl)-4-iodobenzene using 4,4’-difluorodiphenylsulfone (DFDPS) and Bisphenol A in the ratio 25:75:100. Iodo monomer was prepared by reacting 3,5-difluorodiphenyl sulfone with N-iodo succinimide (NIS). Using GC/MS and NMR spectroscopy, the quantitative analysis was done to this monomer. Modification was done to iodo copolymer using sodium azide and copper sulfate as catalyst to form azide copolymer. Further modification of the azide copolymer to triazole copolymers via Click Chemistry using different alkynes such as 1-octyne, phenyl acetylene, propargyl alcohol and acetylenedicarboxylic acid. Polymers characterization was done …

Biophysical Characterization Of A Proline Mutant Of Neural Cadherin, Keshia S. Dykes

Electronic Theses and Dissertations

Cadherins are calcium dependent glycoproteins whose homophilic interactions mediate cell-cell adhesion in solid tissues. They are comprised of an extracellular region, a transmembrane region and a cytoplasmic region. The extracellular region plays a critical role in cadherin-mediated cell adhesion, and has five tandemly repeated ectodomains (ec1-ec5), with three calcium binding sites situated in each interface between the domains. Dimerization of cadherin occurs through formation of adhesive interactions between extracellular domains of cadherins from neighboring cells. Adhesive interaction occurs at the interfaces of ec1 domains of two molecules originating from different cell surfaces. Dimerization is critically dependent on the binding of …

Novel Cell Penetrating Peptide-Adaptors Effect Intracellular Delivery And Endosomal Escape Of Protein Cargos, John C. Salerno, Verra M. Ngwa, Scott J. Nowak, Carol A. Chrestensen, Allison N. Healey, Jonathan L. Mcmurry

Faculty Articles

The use of cell penetrating peptides (CPPs) as biomolecular delivery vehicles holds great promise for therapeutic and other applications, but development has been stymied by poor delivery and lack of endosomal escape. We have developed a CPP-adaptor system capable of efficient intracellular delivery and endosomal escape of user-defined protein cargos. The cell penetrating sequence of HIV transactivator of transcription was fused to calmodulin, which binds with subnanomolar affinity to proteins containing a calmodulin binding site. Our strategy has tremendous advantage over prior CPP technologies because it utilizes high affinity noncovalent, but reversible coupling between CPP and cargo. Three different cargo …