Chemistry Commons^™

The Ligase Detection Reaction: The Evolution Of A Mutation Detection Strategy, Hannah D. Farquar

LSU Doctoral Dissertations

Early detection of genetic mutations is important for control of diseases such as cancer and Alzheimer's. Early detection requires methods that detect small amounts of mutated DNA in very large amounts of normal or wild type DNA. One method to detect mutated DNA is the ligase detection reaction (LDR). Since its inception LDR has evolved greatly from a simple detection reaction after PCR amplification to PCR/RE/LDR, a scheme which uses nucleoside base analogs in PCR to convert wild type sequences to sequences containing restriction endonuclease (RE) sites which can then be cleaved leaving only mutant sequences for detection by LDR. …

Synthesis And Study Of New Materials For The Visual Detection Of Biomolecules, Ming He

LSU Doctoral Dissertations

The visual determination of saccharides has been of interest for well over a century. Sugars are a relatively challenging class of compounds to analyze. They exhibit great structural similarity as well as transparency in the visible region. The sensing of specific saccharides could aid the monitoring of disease or industrial fermentation products. Herein I present new methods allowing for simple and rapid visual detection of sialic acid, fructose and a homologous series of prototypical neutral oligosaccharides, the linear maltodextrins, containing up to seven glucose residues. The new methods allow for selective color detection of fructose in a large excess of …

The Synthesis Of New Biotin Derivatives And Their Bioactivity, Latisha Chanette Johnson

LSU Master's Theses

Biotin is an essential growth factor found in all living cells. It functions as a cofactor for a group of coenzymes that catalyze transcarboxylation, decarboxylation, and carboxylation reactions. Acetyl CoA carboxylase catalyzes the first committed and regulated step in fatty acid synthesis in which malonyl CoA is the product. The biotin-dependent enzyme is found in all animals, plants, and bacteria. We studied the kinetic and structural aspects of acetyl CoA carboxylase, and in 1999 we reported the bioactivity and total synthesis of a unique first generation biotin-derived inhibitor of acetyl CoA carboxylase, 1(BP1). Kinetic studies on the activity of E. …

The Radiation-Induced Degradation Of Poly(Diene Sulfones) As X-Ray Resists, Gina Marie Calderon

LSU Master's Theses

The microlithographic process, essential in the fabrication of silicon chip integrated circuits, uses high-energy radiation to transfer a pattern onto a thin film of polymer resist. Pattern transfer occurs by modifying the properties (solubility or volatility) of the polymer film exposed to radiation. Poly(olefin sulfones) exhibit a high sensitivity to x-rays, which is a desirable property for polymer resists, but typically undergo a glass transition around room temperature and a thermal degradation at moderate temperatures. The thermal properties of the poly(olefin sulfones) reduce the processing latitude for industrial microelectronics applications. Poly(unsaturated olefin sulfones) containing a carbon-carbon double bond in the …

Peroxynitrite-Mediated Oxidations: Nitration And Nitrosation, Rachel Bolzan

LSU Doctoral Dissertations

Using a direct ultraviolet second-derivative spectroscopy method, three peroxynitrite preparative methods were investigated for the nitrite and nitrate present either as impurities or produced during peroxynitrite decomposition: (I) ozonation of azide, reaction of hydrogen peroxide with (II) isoamyl nitrite and (III) nitrous acid. The oxidation of morpholine by peroxynitrite in the presence and absence of added carbonate gives N-nitromorpholine and N-nitrosomorpholine. Nitration and nitrosation of morpholine are catalyzed by low levels of CO₂; however, excess CO₂ dramatically reduces the yields of nitrosation but not nitration, and the combined yields of the products are about the same under …

Electronic And Structural Properties Of Rare Gas Cation Clusters, Jose Alejandro Gascon

LSU Doctoral Dissertations

We study structure and ground and excited state properties of rare gas cation clusters, Rg⁺_N, for N=3-60. The main goal is to understand how the positive charge is delocalized over the cluster and the relationship between cluster geometry and delocalization. He⁺_N, Ar⁺_N, and Xe⁺_N are selected as representatives of the rare gas elements. We perform Monte Carlo simulations to obtain finite temperature properties of the energy spectrum. The Hamiltonian of the system is based on a semi-empirical model whose parameters are obtained through the fitting of experimental and calculated …

X-Ray Spectroscopy Of Geometrically Constrained Systems, Edward Earl Doomes

LSU Doctoral Dissertations

Hard X-rays (2 KeV or greater) are used as spectroscopic tools in the characterization of chemical systems. X-ray absorption near edge structure (XANES) spectroscopy is used to characterize multiple groups of chemicals in a systematic fashion. This thesis focuses on chemical systems that in some context have geometrical constraints on their structure. They include self-assembled n-alkanethiol monolayers that are tethered to silver substrates, cyclic sulfides, and (Bis(diphenylphosphino) alkane tetracarbonyl metal complexes. The study of n-alkanethiol monolayers on Ag(111) contains the first observation in the literature of an anomalous resonance in the 18 carbon monolayer. A new interpretation of a subthreshold …

Precursors For Copper Chemical Vapor Deposition, Muna Bufaroosha

LSU Doctoral Dissertations

The main objective of this study was to synthesize precursors that are capable of producing copper films of high quality by chemical vapor deposition (CVD). We investigated some copper(I) and copper(II) complexes as precursors for chemical or photochemical vapor deposition. In chapter 2, we synthesized a series of Cu(hfac)₂(amine) adducts, where hfac^- is hexafluoroacetylacetonate and the amines are: dimethylamine, isopropylamine, allylamine, pyrrolidine, and piperidine. The efficiency of these adducts compared to Cu(hfac)₂(H₂O) as Cu-CVD precursors was examined under hydrogen. We found that among these amine adducts, Cu(hfac)₂(allylamine)₂ gave the best …

Mode Specific Photoionization Dynamics In Polyatomic Molecules, Gerald Jeffery Rathbone

LSU Doctoral Dissertations

The work presented in this dissertation presents new work on polyatomic photoionization. In these investigations, the broad range behavior of both allowed and forbidden vibrational modes in linear triatomic systems were studied to understand mode specific aspects of photoionization. The current study is made possible by the experimental strategy of exploiting high resolution photoelectron spectroscopy and the high brightness of third generation synchrotron radiation sources. The data is taken typically tens of eV’s past the ionization potential. The strategy that I employ is to probe alternative vibrational modes which are frequently affected differently following resonant ionization. Such vibrationally resolved data …

Capillary And Microdevice Electrophoretic Tools For Genetic Analyses: Heteroduplex Analysis For Tuberculosis Drug Susceptibility And Ligase Detection Reaction For Colorectal Cancer Detection, Gloria Thomas

LSU Doctoral Dissertations

The traditional format of electrophoresis, the slab gel, is quickly being replaced by capillary and microdevice platforms, which offer improvements in cost, resolution, speed, quantitation and automation in genetic analyses. These techniques also employ a variety of separation matrices while the slab gel is limited to agarose and polyacrylamide. The research presented here explores the use of these electrophoretic formats for the detection of single nucleotide mutations using two genetic models associated with human disease. Slab gel based heteroduplex analysis (HDA), a popular mutation scanning method, uses a specially designed universal heteroduplex generator (UHG) containing controlled variation to enhance the …

Artificial Peptides Containing Ca,A- Disubstituted Amino Acids: Synthesis, Conformational Studies, And Application As Β-Strand Mimics, Yanwen Fu

LSU Doctoral Dissertations

Short peptides containing C^α,α-dipropylglycine (Dpg) at alternating sequence positions were synthesized and examined for conformational behavior. Peptide assembly was performed using Fmoc-solid-phase chemistry where the coupling with PyAOP could be significantly enhanced at elevated temperature. Circular dichroism (CD) and NMR conformational studies revealed that incorporation of Dpg residues induced folded structures into peptides. It was observed that Dpg residues adopted helical conformation in a helix-promoting sequence. The resulting helical structure was comprised of consecutive β-turns whose structure was stabilized by salt bridge in aqueous solution. In this study, the preparation of sterically and polyfunctional C^α,α-disubstituted amino …

Fabricating Microfluidic Devices In Polymers For Bioanalytica Applications, Sean M. Ford

LSU Doctoral Dissertations

The research presented in this document focuses on the fabrication, characterization and application of microfluidic systems fabricated in poly(methyl methacrylate) (PMMA) with the emphasis focused on the fabrication processing steps. Microfluidic devices were produced in PMMA using X-ray lithography. The fabrication methods investigated were sacrificial mask, polyimide membrane mask and embossing techniques. PMMA microfluidic devices fabricated using X-ray lithography were characterized using scanning electron microscopy (SEM) and optical microscopy, while analytical techniques such as electroosmotic flow determination, separations, and fluorescent microscopy were used to characterize fluid transport in these devices. A novel method for the heat annealing of PMMA to …

Synthesis, Characterization, And Applications Of Novel Pseudostationary Phases In Micellar Capillary Electrophoresis For Separation Of Chiral And Archiral Compounds H[Electronic Resource], Cevdet Akbay

LSU Doctoral Dissertations

The research presented in this dissertation involves the synthesis, characterization, and the use of novel surfactants, including both micelles and vesicles, as pseudostationary phases in micellar capillary electrophoresis (MCE) for the separation of achiral and chiral compounds. Separation of environmental pollutants such as 2 to 6-ring polycyclic aromatic hydrocarbons (PAHs) was achieved using poly(sodium undecylenic sulfate). A baseline separation of all 16 PAHs was possible for the first time in MCE by a single-surfactant system. In addition, a surfactant with a phosphated head group, i.e., di(2-ethylhexyl)phosphate (DEHP), was also introduced as a novel pseudostationary phase for separation of 21 weakly …

Analysis Of Near-Infrared Dye-Labeled Sanger Sequencing Fragments With Gel Electrophoresis Using The Time-Resolved Flourescence Lifetime Indentification Methods, Suzanne Jeanel Lassiter

LSU Doctoral Dissertations

The research presented in this dissertation involves the identification of sequencing fragments with time-resolved methods. For this application, near-infrared heavy-atom tricarbocyanine dyes were developed in our laboratory, which can be excited with a single laser and emission collected using a single detection channel. The dyes have four spectroscopically unique, but relatively short lifetimes that can be altered by the intramolecular heavy-atom they contain. The work described here involves the optimization of dye-primer chemistry for preparing Sanger sequencing reactions for longer reads and the optimization of the separation matrix for capillary gel electrophoresis that produces favorable statistical analysis of the aforementioned …

Synthesis And Analysis Of Three-Dimensional Molecular Architectures, Patricia Beck

LSU Master's Theses

The use of resorcinarenes in the study of biologically significant phenomena has been the focus of much attention, due to the versatility of these macrocyclic compounds. In this thesis, two applications of resorcinarenes are explored. First, the search for the mechanism by which tetraarylboronic acid resorcinarenes lead to colorimetric detection and quantization of saccharide analyte concentration in solution is described. Second, the extension of the lower rim conjugation of the resorcinarene macrocycle by the fourfold addition of various length oligo(p-phenylene) rods is discussed. The synthesis of these tubular materials, with potential uses as artificial ion channels and for binding and …

The Synthesis Of Deuterated Arylamine Dna Adducts For Use In The Development Of An Isotope Dilution Lc/Ms/Ms Method, Philip Dmitri Olsen

LSU Doctoral Dissertations

Polycyclic aromatic amines (arylamines) are a class of chemical carcinogens that are prevalent in environmental and industrial settings. In order to study the mechanism of their toxicity a quantitative and qualitative detection method was developed to measure the C8-adenine adducts of benzidine and 2-aminofluorene in DNA samples. To do this a novel synthetic method using a palladium catalyst was developed to prepare authentic and deuterated arylamine adducts to serve as standards. These standards were then used to develop a high performance liquid chromatography, electrospray, tandem mass spectrometry, isotope dilution, detection method. To demonstrate the validity of this method two spike …

Analysis Of Inorganic Materials Using Advanced Nmr And Nqr Techniques, Anthony Adrian Mrse

LSU Doctoral Dissertations

Several advanced solid-state nuclear magnetic resonance (NMR) and nuclear quadrupole resonance (NQR) techniques have been applied to inorganic materials of commercial interest. The specific techniques applied to each system were chosen to solve specific obstacles which impeded the flow of industrial science.

First, the dispersion of brominated flame retardants in polymers is monitored using pulsed ⁸¹Br NQR (nuclear quadrupole resonance) techniques. The NQR spectrometer consists of a homemade NMR console and an automatically tuned loop-gap resonator probe. The two factors which affect the ⁸¹Br NQR transition frequencies of brominated aromatics are: electron-donating and withdrawing substituents on the ring …

Synthesis Of A Novel ??-Amino Acid Capable Of Stabilizing A 310-Helix Via Side-Chain Salt-Bridging, Reema K. Thalji

Honors Capstones

No abstract provided.

Synthesis Of 1-Alloc-Piperidine-4-Fmoc-Amino-4-Carboxylic Acid: A Novel, Protected ?,?- Disubstituted Amino Acid, Muna K. Thalji

Honors Capstones

No abstract provided.

The Effects Of Free Fatty Acids And Tocopherol On Membrane Order And Antioxidant Activity, Gina Michelle Pineda

Honors Capstones

No abstract provided.

The Synthesis Of Amino Acid Derivatives For Peptide Incorporation, Yvette Delahoussaye

Honors Capstones

No abstract provided.

New Methods For The Study Of Fast Electrode Reactions., Talivaldis Berzins

LSU Historical Dissertations and Theses

No abstract provided.

The Construction Of A Recording Raman Spectrometer And An Analysis Of The Vibrational Spectrum Of Bromobenzene And Its Monodeuterium Isomers., William Ward Daniels

LSU Historical Dissertations and Theses

No abstract provided.

Chromatographic Studies., Richard Thomas Moravek

LSU Historical Dissertations and Theses

No abstract provided.

Voltammetry At Constant Current And Its Application To Electrochemical Kinetics., Calvin C. Mattax

LSU Historical Dissertations and Theses

No abstract provided.

Studies Of The Reactions In Alcoholic Solution Of Gold(Iii) And Alpha-Naphthylamine., Theo Conner Mccoy

LSU Historical Dissertations and Theses

No abstract provided.

A Study Of Some Nitroso Compounds., Cilton Watson Tate

LSU Historical Dissertations and Theses

No abstract provided.

Analytical Studies Of Some Acyloin-Oximes., Hans A. Suter

LSU Historical Dissertations and Theses

No abstract provided.

A Study Of Line-Width Photographic Photometry For Use In Spectrochemical Analysis., John A. Perry

LSU Historical Dissertations and Theses

No abstract provided.

Electrokinetic And Light Scattering Study Of Alumina Sols., James Porter Craig

LSU Historical Dissertations and Theses

No abstract provided.