Chemistry Commons^™

Use Of Affinity Chromatography In Developing Acridinium Ester-Labeled Antibodies For An Immunometric Assay Of Parathyrin, David S. Hage, Bob Taylor, Pat Schryver, Pai C. Kao

David Hage Publications

In developing an immunometric assay of intact parathyrin (parathyroid hormone, PTH), we found that affinity chromatography is a useful tool in purifying and optimizing the labeling conditions for acridinium ester-labeled antibodies. ... In summary, affinity chromatography was found to be useful in the purification of acridinium ester-labeled antibodies, particularly for removing denatured antibodies from the prepared label and for monitoring the amount of active labeled antibodies produced.

A Prostaglandin Synthase Inhibition Assay With Detection By Elisa, Patricia Scott

Theses & Honors Papers

A simple, reliable method is described for the routine measurement of non-steroidal anti-inflammatory drugs (NSAIDs) as inhibitors of prostaglandin synthase. Appropriate concentrations of inhibitor are incubated with ram seminal vesical prostaglandin synthase, sodium arachidonate and reduced glutathione. Prostaglandin E₂ (PGE₂) production is then quantitated by and enzyme linked immunosorbant assay (ELISA), in which anti-PGE₂ antibody is utilized in a binding competition between test sample and adsorbed conjugate of PGE₂-BSA. The antibody which remains bound to the solid phase is quantitatively determined colorimetrically by incubation with horse radish peroxidase labeled goat anti-rabbit IgG followed by …

Telomere Structure In Euplotes Crassus: Characterization Of Dna-Protein Interactions And Isolation Of A Telomere-Binding Protein, Carolyn M. Price

Department of Chemistry: Faculty Publications

The nucleoprotein structure of telomeres from Euplotes crassus was studied by using nuclease and chemical footprinting. The macronuclear telomeres were found to exist as DNA-protein complexes that are resistant to micrococcal nuclease digestion. Each complex encompassed 85 to 130 base pairs of macronuclear DNA and appeared to consist of two structural domains that are characterized by dissimilar DNA-protein interactions. Dimethyl sulfate footprinting demonstrated that very sequence-specific and salt-stable interactions occur in the most terminal region of each complex. DNase I footprinting indicated that DNA in the region 30 to 120 base-pairs from the 5' end lies on a protein surface; …

7li Nmr Of Normal Human Erythrocytes, Rao P. Gullapalli, Roger M. Hawk, Richard A. Komoroski

Journal of the Arkansas Academy of Science

Lithium has been known to be an effective medication for people with bipolar disorder. The mechanisms of action of lithium in the brain is not very well understood. NMR spectroscopy and imaging are effective both in determining lithium levels in tissue and brain. We have monitored lithium levels in red blood cells. We have been able to separate intra- and extracellular compartments of lithium using shift reagents, thereby obtaining T^1 's of both the compartments. Lithium uptake as a function of hematocrit was monitored weekly over a 3 week period. The time constant of 50 mM lithium uptake at 25°C …

The Synthesis And Evaluation Of 5-Phenyloxazolidines As Potential Cardiovascular Drugs, Tammy C. Wang

Chemistry & Biochemistry Theses & Dissertations

Drugs such as adrenoceptor antagonists and certain centrally acting agents are known to lower blood pressure and thus serve as clinically useful antihypertensive agents. Norepinephrine 4, a natural ∝-adrenoceptor agonist, is the major agent associated with increased blood pressure in the periphery. Based on a careful structure-activity comparison, a series of substituted oxazolidines, 20, 21, 22 and 23, have been designed as possible antihypertensive candidates. Specifically, 22 and 23 have been synthesized to serve as potential norepinephrine antagonists.

Preliminary evaluation of these compounds indicated that they were not antihypertensive in nature. The more active compound, 23, …

Gas Phase Hydrogen Permeation In Alpha Titanium And Carbon Steels, Donald L. Johnson, Kiritkumar K. Shah, Bruce H. Reeves, Vijay L. Gadgeel

Department of Chemistry: Faculty Publications

Commercially pure titanium rods were machined into hollow cylinders and gas-phase permeation and diffusion measurements obtained with a quadrupole residual gas analyzer at temperatures between 400-800°C and pressures between 0.533 and 266.6 Nrn^-2. Pressure dependence measurements established that phase boundary reactions as well as lattice diffusion control the transport process. Lag-time data yield excellent agreement with the diffusivity data of Wasilewski and Kehl and Papazoglou and Hepworth for the case where phase boundary reactions are taken into account. The best value of the diffusivity is D = 2.1 x 10^-2 exp (-50,40O/RT). Analysis of lag-time data reveals …

Pulling The Wool Off, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

The Department of Agriculture has begun a three year project to study the potential for "chemical shearing" or, more correctly, biological defleecing.

Oxygen Consumption By Acrylamide Polymerization For Rapid Screening Of Chemotherapeutic Agents, Richard A. Johnson '74

Fenwick Scholar Program

This paper describes a screening process used to determine the overall effect of anti-cancer drugs on a tumor more rapidly than other established methods. This technique measures the rate of oxygen consumption by cells when a solution of acrylamide and bisacrylamide is polymerized.

Comparative Phytochemistry Of Saururaceae Essential Oils., Lohit Venkateswara Tutupalli

University of the Pacific Theses and Dissertations

In view of their native medicinal uses, a systematic investigation of the phytochemistry and Hippocratic screening of three members of the Saururaceac namely, Anemopsis californica, Saururus cernuus and Houttuynia cordata was undertaken.

Xanthine Oxidase Catalyzed Oxidation Of Acetaldehyde Substrate And Carboxylate Inhibition, Patrick A. Goodman

All Master's Theses

In recent years, interest in an enzyme which catalyzes the oxidation of a variety of aldehydes and purines has been growing steadily. In fact, this enzyme commonly known as xanthine oxidase, has been implicated by some (1) in the regulation of nucleic acid synthesis and cellular growth - an important consideration in the study of caner. Unfortunately, detailed investigations directly pertaining to biological aspects of this enzyme have as yet been somewhat limited. Nevertheless, the amount of information concerning the nature of the enzyme and its catalytic effects upon different classes of substrates, through far from complete, is indeed significant.

The Effects Of Indomethacin On A Strain Of Fibroblasts Cultivated In Vitro, John Thomas Mccloskey

University of the Pacific Theses and Dissertations

Man has always searched for greater understanding of himself and his environment. As a result, much scientific investigation has been directed towards the understanding of the life process. In recent years, such scientific inquiry has become increasingly focused on the fundamental biochemical events taking place at the cellular, subcellular, and molecular levels. In the course of investigation into the nature of living things, research has often necessitated the development of new techniques and investigational tools to serve as experimental systems. Very often such new methods have made possible the discovery of previously unknown processes. One very interesting and useful investigational …

The Use Of Pickled Wheat In Poultry Feeding, P Smetana

Journal of the Department of Agriculture, Western Australia, Series 4

FEEDING fungicides to poultry can result in harmful effects, to the birds themselves, and to their human consumers.

To prevent such effects it is advisable that wheat pickled with fungicides should not be included in poultry rations.

Itaconic Acid Derivatives Of Sulfanilamide And Sulfone, Malcolm J. Thompson

Electronic Theses and Dissertations

This thesis, submitted to Xavier University in partial fulfillment of requirements for a Maters degree in chemistry in 1952, reports the synthesis and characterization of itaconic acid derivatives of sulfanilamide and amino phenyl sulfone compounds. The research describes reaction procedures and the isolation and purification of a series of novel chemical compounds, with experimental data including melting points, percent yields, elemental analysis, and molecular weights reported for numerous derivatives. The thesis presents conclusions regarding the chemical properties and reactivity of the synthesized compounds.

Note: No original abstract was included; this abstract is provided by the XULA Digital Commons editors.