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Modification Of A Capillary For Electrophoresis By Electrostatic Self-Assembly Of An Enzyme For Selective Determination Of The Enzyme Substrate, Stephanie Hooper, Mark Anderson

Mark R. Anderson

The integration of a separation capillary for capillary electrophoresis (CE) with an on-column enzyme reaction for selective determination of the enzyme substrate is described. Enzyme immobilization is achieved by electrostatic assembly of poly(diallydimethylammonium chloride) (PDDA) followed by adsorption of a mixture of the negatively charged enzyme glucose oxidase (GOx) and anionic poly(styrenesulfonate) (PSS). The reaction of glucose with the GOx produces hydrogen peroxide which migrates the length of the capillary and is detected amperometrically at the capillary outlet. The enzyme reaction occurs during a capillary separation, allowing selective determination of the substrate in complex samples without the need for pre- …