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Potential Driven Deposition Of Poly(Diallyldimethylammonium Chloride) Onto The Surface Of 3-Mercaptopropionic Acid Monolayers Assembled On Gold, Wesley Sanders, Mark Anderson
Potential Driven Deposition Of Poly(Diallyldimethylammonium Chloride) Onto The Surface Of 3-Mercaptopropionic Acid Monolayers Assembled On Gold, Wesley Sanders, Mark Anderson
Mark R. Anderson
Electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance (QCM) measurements are used to examine the ability of applied potential to drive the ionic self-assembly of poly(diallyldimethylammonium) chloride (PDDA) onto a substrate modified with a monolayer of 3-mercaptopropionic acid (3-MPA). The potential of zero charge (PZC) of the gold electrode modified with a monolayer of 3-MPA was found by differential capacitance measurements to be −0.12 (±0.01) V versus Ag−AgCl. Changing the substrate potential to values positive (−0.01 V vs Ag−AgCl) of the PZC induces interfacial conditions that are favorable for the electrostatic deposition of cationic polymers onto the surface of 3-MPA …
Modification Of A Capillary For Electrophoresis By Electrostatic Self-Assembly Of An Enzyme For Selective Determination Of The Enzyme Substrate, Stephanie Hooper, Mark Anderson
Modification Of A Capillary For Electrophoresis By Electrostatic Self-Assembly Of An Enzyme For Selective Determination Of The Enzyme Substrate, Stephanie Hooper, Mark Anderson
Mark R. Anderson
The integration of a separation capillary for capillary electrophoresis (CE) with an on-column enzyme reaction for selective determination of the enzyme substrate is described. Enzyme immobilization is achieved by electrostatic assembly of poly(diallydimethylammonium chloride) (PDDA) followed by adsorption of a mixture of the negatively charged enzyme glucose oxidase (GOx) and anionic poly(styrenesulfonate) (PSS). The reaction of glucose with the GOx produces hydrogen peroxide which migrates the length of the capillary and is detected amperometrically at the capillary outlet. The enzyme reaction occurs during a capillary separation, allowing selective determination of the substrate in complex samples without the need for pre- …