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Articles 91 - 97 of 97
Full-Text Articles in Physical Sciences and Mathematics
Helicase-Binding To Dnai Exposes A Cryptic Dna-Binding Site During Helicase Loading In Bacillus Subtilis, Charikleia Ioannou, Patrick M. Schaeffer, Nicholas E. Dixon, Panos Soultanas
Helicase-Binding To Dnai Exposes A Cryptic Dna-Binding Site During Helicase Loading In Bacillus Subtilis, Charikleia Ioannou, Patrick M. Schaeffer, Nicholas E. Dixon, Panos Soultanas
Faculty of Science - Papers (Archive)
The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. Here we show that DnaI consists of a C-terminal domain (Cd) with ATPase and DNA-binding activities and an N-terminal domain (Nd) that interacts with the replicative ring helicase. A Zn21-binding module mediates the interaction with the helicase and C67, C70 and H84 are involved in the coordination of the Zn21. DnaI binds ATP and exhibits ATPase activity that is not stimulated by ssDNA, because the DNAbinding site on Cd is masked by Nd. The ATPase activity resides on the …
Identification Of The Rest Regulon Reveals Extensive Transposable Element-Mediated Binding Site Duplication, Rory Johnson, Richard J. Gamblin, Lezanne Ooi, Alexander W. Bruce, Ian J. Donaldson, David R. Westhead, Ian Wood, Richard M. Jackson, Noel J. Buckley
Identification Of The Rest Regulon Reveals Extensive Transposable Element-Mediated Binding Site Duplication, Rory Johnson, Richard J. Gamblin, Lezanne Ooi, Alexander W. Bruce, Ian J. Donaldson, David R. Westhead, Ian Wood, Richard M. Jackson, Noel J. Buckley
Faculty of Science - Papers (Archive)
The genome-wide mapping of gene-regulatory motifs remains a major goal that will facilitate the modelling of gene-regulatory networks and their evolution. The repressor element 1 is a long, conserved transcription factor-binding site which recruits the transcriptional repressor REST to numerous neuron-specific target genes. REST plays important roles in multiple biological processes and disease states. To map RE1 sites and target genes, we created a position specific scoring matrix representing the RE1 and used it to search the human and mouse genomes. We identified 1301 and 997 RE1s inhuman and mouse genomes, respectively, of which >40% are novel. By employing an …
Annotated Records Of The Greater Glider, Petauroides Volans, From The Victorian Naturalist 1884-2005, K Shane Maloney, Jamie M. Harris
Annotated Records Of The Greater Glider, Petauroides Volans, From The Victorian Naturalist 1884-2005, K Shane Maloney, Jamie M. Harris
Faculty of Science - Papers (Archive)
A survey of The Victorian Naturalist was undertaken for records of the Greater Glider Petauroides volans. This report compiles around 52 distribution records, and summarises naturalists’ observations of the animal’s behaviour and feeding. Those concerned with the ecology and conservation of arboreal marsupials generally, and the Greater Glider in particular, should find this work useful as it compiles many interesting and important records of this the largest and most conspicuous of the gliding possums.
Annotated Records Of The Feathertail Glider, Acrobates Pygmeus, From The Victorian Naturalist, Jamie M. Harris, K Shane Maloney
Annotated Records Of The Feathertail Glider, Acrobates Pygmeus, From The Victorian Naturalist, Jamie M. Harris, K Shane Maloney
Faculty of Science - Papers (Archive)
The Victorian Naturalist was surveyed for past records of the Feathertail Glider Acrobates pygmaeus. We document many important records of their occurrence, as well as accounts on their feeding and behaviour. This report should be useful to researchers seeking primary source observations of this species.
Comparison Of Virulence Gene Profiles Between Escherichia Coli Strains Isolated From Healthy And Diarrheic Swine, Mark R. Wilson, Xi Yang Wu, Idris Barchia, Karl A Bettelheim, Steven Driesen, Darren J. Trott, Toni A. Chapman, James J C Chin
Comparison Of Virulence Gene Profiles Between Escherichia Coli Strains Isolated From Healthy And Diarrheic Swine, Mark R. Wilson, Xi Yang Wu, Idris Barchia, Karl A Bettelheim, Steven Driesen, Darren J. Trott, Toni A. Chapman, James J C Chin
Faculty of Science - Papers (Archive)
A combination of uni- and multiplex PCR assays targeting 58 virulence genes (VGs) associated with Escherichia coli strains causing intestinal and extraintestinal disease in humans and other mammals was used to analyze the VG repertoire of 23 commensal E. coli isolates from healthy pigs and 52 clinical isolates associated with porcine neonatal diarrhea (ND) and postweaning diarrhea (PWD). The relationship between the presence and absence of VGs was interrogated using three statistical methods. According to the generalized linear model, 17 of 58 VGs were found to be significant (P < 0.05) in distinguishing between commensal and clinical isolates. Nine of the 17 genes represented by iha, hlyA, aidA, east1, aah, fimH, iroN(E. coli), traT, and saa have not been previously identified as important VGs in clinical porcine isolates in Australia. The remaining eight VGs code for fimbriae (F4, F5, F18, and F41) and toxins (STa, STb, LT, and Stx2), normally associated with porcine enterotoxigenic E. coli. Agglomerative hierarchical algorithm analysis grouped E. coli strains into subclusters based primarily on their serogroup. Multivariate analyses of clonal relationships based on the 17 VGs were collapsed into two-dimensional space by principal coordinate analysis. PWD clones were distributed in two quadrants, separated from ND and commensal clones, which tended to cluster within one quadrant. Clonal subclusters within quadrants were highly correlated with serogroups. These methods of analysis provide different perspectives in our attempts to understand how commensal and clinical porcine enterotoxigenic E. coli strains have evolved and are engaged in the dynamic process of losing or acquiring VGs within the pig population.
Dust Deposition Rates In The Wollongong-Port Kembla Area, New South Wales, Australia, R J. Morrison, Bandar A. Fadhel, K Goss
Dust Deposition Rates In The Wollongong-Port Kembla Area, New South Wales, Australia, R J. Morrison, Bandar A. Fadhel, K Goss
Faculty of Science - Papers (Archive)
Dust deposition in the Wollongong-Port Kembla region, New South Wales, Australia, arising from local industrial and mining activities, has been of major concern since the early 1960s. Reports dealing with dust deposition rates in the region have been published by different organisations where the data have been averaged for the region. This provides a general trend for the deposition rates for the whole region without considering the trends occurring in specific locations. This study was the first to examine the trends observed at 35 individual gauges to identify more localised trends in dust deposition rates in the Wollongong-Port Kembla region, …
Investigation Of Large-Scale Washover Of A Small Barrier System On The Southeast Australian Coast Using Ground Penetrating Radar, Brian G. Jones, C Bristow, Adam D. Switzer
Investigation Of Large-Scale Washover Of A Small Barrier System On The Southeast Australian Coast Using Ground Penetrating Radar, Brian G. Jones, C Bristow, Adam D. Switzer
Faculty of Science - Papers (Archive)
Prehistoric depositional signatures for large-scale washover involving marine inundation events such as storm and tsunami have been the subject of considerable research over the last 15 years. Much of this research has focused on the identification of sand sheets in back-barrier environments as depositional records for extreme washover events. All these deposits must have a sediment source, and by their nature, the most likely source of sediment for washover into back-barrier environments is the barrier itself. This study identifies an erosional signature for large-scale washover from a small coastal barrier on the southeast Australian coast. A distinct lense of marine …