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Full-Text Articles in Physical Sciences and Mathematics
The Maintenance Of High Affinity Plasminogen Binding By Group A Streptococcal Plasminogen-Binding M-Like Protein Is Mediated By Arginine And Histidine Residues Within The A1 And A2 Repeat Domains., Martina L. Sanderson-Smith, Mark J. Walker, Marie Ranson
The Maintenance Of High Affinity Plasminogen Binding By Group A Streptococcal Plasminogen-Binding M-Like Protein Is Mediated By Arginine And Histidine Residues Within The A1 And A2 Repeat Domains., Martina L. Sanderson-Smith, Mark J. Walker, Marie Ranson
Faculty of Science - Papers (Archive)
Subversion of the plasminogen activation system is implicated in the virulence of group A streptococci (GAS). GAS displays receptors for the human zymogen plasminogen on the cell surface, one of which is the plasminogen-binding group A streptococcal M-like protein (PAM). The plasminogen binding domain of PAM is highly variable, and this variation has been linked to host selective immune pressure. Site-directed mutagenesis of full-length PAM protein from an invasive GAS isolate was undertaken to assess the contribution of residues in the a1 and a2 repeat domains to plasminogen binding function. Mutagenesis to alanine of key plasminogen binding lysine residues in …
Trigger For Group A Streptococcal M1t1 Invasive Disease, J. N. Cole, Jason D. Mcarthur, F. C. Mckay, Martina L. Sanderson-Smith, Amanda J. Cork, Marie Ranson, M. Rohde, A. Itzek, H. Sun, D. Ginsburg, M. Kotb, V. Nizet, G. S. Chhatwal, Mark J. Walker
Trigger For Group A Streptococcal M1t1 Invasive Disease, J. N. Cole, Jason D. Mcarthur, F. C. Mckay, Martina L. Sanderson-Smith, Amanda J. Cork, Marie Ranson, M. Rohde, A. Itzek, H. Sun, D. Ginsburg, M. Kotb, V. Nizet, G. S. Chhatwal, Mark J. Walker
Faculty of Science - Papers (Archive)
The globally disseminated Streptococcus pyogenes M1T1 clone causes a number of highly invasive human diseases. The transition from local to systemic infection occurs by an unknown mechanism; however invasive M1T1 clinical isolates are known to express significantly less cysteine protease SpeB than M1T1 isolates from local infections. Here, we show that in comparison to the M1T1 strain 5448, the isogenic mutant ∆speB accumulated 75-fold more human plasmin activity on the bacterial surface following incubation in human plasma. Human plasminogen was an absolute requirement for M1T1 strain 5448 virulence following subcutaneous infection of humanized plasminogen transgenic mice. S. pyogenes M1T1 isolates …
Domains Of Group A Streptococcal M Protein That Confer Resistance To Phagocytosis, Opsonization And Protection: Implications For Vaccine Development., Jason D. Mcarthur, Mark J. Walker
Domains Of Group A Streptococcal M Protein That Confer Resistance To Phagocytosis, Opsonization And Protection: Implications For Vaccine Development., Jason D. Mcarthur, Mark J. Walker
Faculty of Science - Papers (Archive)
Streptococcus pyogenes (group A streptococcus) colonises skin and throat tissues resulting in a range of benign and serious human diseases. Opsonisation and phagocytosis are important defence mechanisms employed by the host to destroy group A streptococci. Antisera against the cell-surface M protein, of which over 150 different types have been identified, are opsonic and contribute to disease protection . In this issue of Molecular Microbiology, Sandin and colleagues have comprehensively analysed the regions of M5 protein that contribute to phagocytosis resistance and opsonisation. Human plasma proteins bound to M5 protein B- and C-repeats were shown to block opsonisation, an observation …