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Full-Text Articles in Physical Sciences and Mathematics
Identification Of Fibroblasts As A Major Site Of Albumin Catabolism In Peripheral Tissues, Jeffrey L. Strobel, Susan G. Cady, Thomas K. Borg, Louis Terracio, John W. Baynes, Suzanne R. Thorpe
Identification Of Fibroblasts As A Major Site Of Albumin Catabolism In Peripheral Tissues, Jeffrey L. Strobel, Susan G. Cady, Thomas K. Borg, Louis Terracio, John W. Baynes, Suzanne R. Thorpe
Faculty Publications
Rat serum albumin has been labeledw ith dilactitollZ5I- tyramine,( 12‘I-DLT) a radioactive tracer which remains entrappedw ithin lysosomes following cellular uptake and degradation of the carrier protein. Similar kinetics of clearance from the rat circulation were observed for albumin labeled conventionally with lZsI or 12‘I-DLT-albumin, both proteinhsa ving circulating half-lives of -2.2 days. In contrast, the recovery of whole body radioactivity had half-lives of -2.2 and 5.1 days, respectively, for the two protein preparations, indicating substantial retention of degradation products derived from catabolism of ”‘I-DLT-albumin. Measurement of total and acid-soluble radioactivity in tissues 2 or 4 days after injection of …
Identification Of N Epsilon-Carboxymethyllysine As A Degradation Product Of Fructoselysine In Glycated Protein, Mahtab U. Ahmed, Suzanne R. Thorpe, John W. Baynes
Identification Of N Epsilon-Carboxymethyllysine As A Degradation Product Of Fructoselysine In Glycated Protein, Mahtab U. Ahmed, Suzanne R. Thorpe, John W. Baynes
Faculty Publications
The chemistry of Maillard or browning reactionosf glycated proteins was studied using the model compound, Nu-formyl-W-fructoselysine(f FL), an analog of glycated lysine residues in protein. Incubation of fFL (15 mM) at physiological pH and temperature in 0.2 M phosphate buffer resulted in formation of lVcarboxymethyllysine (CML) in about 40% yield after 15 days. CML was formed by oxidative cleavage of fFL between C-2 and C-3 of the carbohydrate chain and erythronic acid (EA) was identified a s , the split product formed in the reaction. Neither CML nor EA was formed from fFL under a nitrogen atmosphere. The rate of …
Analytical Applications Of Absorption Spectroelectrochemistry At Grazing Incidence, Julian Tyson
Analytical Applications Of Absorption Spectroelectrochemistry At Grazing Incidence, Julian Tyson
Chemistry Department Faculty Publication Series
Analytical applications of spectroelectrochemistry are limited by the short path-length, in the absorbing medium, that can be produced with most light-beam/electrode configurations. This disadvantage is overcome for grazing incidence. A cell fitted with glassy-carbon electrodes and used in a conventional spectrophotometer is described and applied to model systems illustrating the use of (a) homogeneous redox reactions, (b) homogeneous redox reaction followed by chemical reaction and (c) electro-deposition followed by stripping into a reagent solution. This last technique is the spectroelectrochemical analogue of anodic-stripping voltammetry.
Peak Width And Reagent Dispersion In Flow Injection Analysis, Julian Tyson
Peak Width And Reagent Dispersion In Flow Injection Analysis, Julian Tyson
Chemistry Department Faculty Publication Series
No abstract provided.