Physical Sciences and Mathematics Commons^™

Synthesis Of Functionalized Resorcin[4]Arene Via Click Chemistry, Ali Husain

USF Tampa Graduate Theses and Dissertations

Click chemistry is a very powerful chemical strategy that overcome carbon-carbon bond with carbon-heteroatom bond by joining small units with heteroatom links (C-X-C) using spring-loaded reactants. The Cu(I)-catalyzed Huisgen 1,3-dipolar cycloaddition is a major example based on the click chemistry philosophy. This method was used for the last 10 years to join different functional groups, carbohydrates, aminoacids, polymers to calix[4]arene and resorcin[4]arene cavitands by a stable 1,2,3-triazole linkages.

Herein I describe our interest in this type of click chemistry reaction in the synthesis of dimeric capsules resorcin[4]arene via four 1,2,3-triazole linkages. Two different resorcin[4]arene derivatives were synthesized in which four …

Architecture And Compiler Support For Leakage Reduction Using Power Gating In Microprocessors, Soumyaroop Roy

USF Tampa Graduate Theses and Dissertations

Power gating is a technique commonly used for runtime leakage reduction in digital CMOS circuits. In microprocessors, power gating can be implemented by using sleep transistors to selectively deactivate circuit modules when they are idle during program execution. In this dissertation, a framework for power gating arithmetic functional units in embedded microprocessors with architecture and compiler support is proposed. During compile time, program regions are identified where one or more functional units are idle and sleep instructions are inserted into the code so that those units can be put to sleep during program execution. Subsequently, when their need is detected …

Design Of A New Suturing And Knot Tying Device For Laparoscopic Surgery, Sinan Onal

USF Tampa Graduate Theses and Dissertations

Minimally invasive or laparoscopic surgery has completely changed the focus of surgery becoming an alternative to various types of open surgery. Minimally invasive surgery avoids invasive open surgery as the operation is performed through one or more small incisions in the abdomen and using a small camera called laparoscope. Through these incisions, surgeons insert specialized surgical instruments to perform the operation resulting in less postoperative pain, shorter hospital stay, and faster recovery. However, the main problems during minimally-invasive surgery are the limited space for operating instruments and the reduced visibility and range of motion inside the patient’s body. During minimally-invasive …

Applications Of Molecular Dynamics Techniques And Spectroscopic Theories To Aqueous Interfaces, Anthony Green

USF Tampa Graduate Theses and Dissertations

The primary goal of spectroscopy is to obtain molecularly detailed information about the system under study. Sum frequency generation (SFG) vibrational spectroscopy is a nonlinear optical technique that is highly interface specific, and is therefore a powerful tool for understanding interfacial structure and dynamics. SFG is a second order, electronically nonresonant, polarization experiment and is consequently dipole forbidden in isotropic media such as a bulk liquid. Interfaces, however, serve to break the symmetry and produce a signal. Theoretical approximations to vibrational spectra of O-H stretching at aqueous interfaces are constructed using time correlation function (TCF) and instantaneous normal mode (INM) …

Design And Combinatorial Synthesis Approach Of Non-Peptidic Trimeric Small Molecules Mimicking I, I + 4(3), I + 7 Positions Of Alpha-Helices, Mingzhou Zhou

USF Tampa Graduate Theses and Dissertations

Protein-protein interactions are key to several biological processes that facilitate signal transduction and many other processes. These interactions are involved in pathways that are critical to many human diseases. Targeting specific protein-protein interactions is a challenging goal because protein-protein interactions are predominately through hydrophobic interactions. Antagonists of the protein-protein interactions need to be perfectly fit into the binding pockets to ensure the activity. The -helical domain of the proteins behaves as the recognition motifs for numerous protein-protein, and protein-nucleic acid interactions. Research has shown that pathways of many diseases contain protein-protein interactions involving -helical domains, e.g. neurological disorders, bacterial infections, …

Design, Combinatorial Synthesis, And Biological Evaluation Of Novel Α-Helical Mimetics Based On Functionalized Piperazines As Antagonists Of P53/Mdm2 Interactions, Melissa Elizabeth Topper

USF Tampa Graduate Theses and Dissertations

The p53 protein promotes tumor eradication upon activation, making it an attractive target in cancer therapies. A reported 50% of all human cancers display aberrant activation of the MDM2 oncoprotein, which directly promotes tumorgenesis by inactivating the transcriptional activity of wild type p53, and is commonly associated with drug, chemo, and radio therapy resistance. Previously reported crystallographic analysis of the p53/MDM2 complex infers that the p53 protein forms a 2.5 turn amphipathic alpha helix whose hydrophobic face interacts within a deep hydrophobic cleft in the NH2-terminal domain of the globular MDM2. This suggests that the synthesis of small molecular antagonists …

The Role Of Rater Motivation In Personnel Selection Validation Studies, Dan Ispas

USF Tampa Graduate Theses and Dissertations

Personnel selection validation studies are routinely conducted in contemporary organizations for selecting and placing employees. Although numerous studies have been conducted with the goal of identifying new predictors, less research was focused on the criterion side. In the current paper, across three studies and five samples, I examined the role played by rater motivation in validation studies. I proposed that rater motivation would impact criterion-related validity of various predictors, the reliability, and the variance of performance ratings. In Study 1, these hypotheses were tested in two samples with varied operationalizations of predictors and of rater motivation. In Study 2, I …

Variable-Density Flow Models Of Saltwater Intrusion In Coastal Landforms In Response To Climate Change Induced Sea Level Rise And A Chapter On Time-Frequency Analysis Of Ground Penetrating Radar Signals, Swagata Guha

USF Tampa Graduate Theses and Dissertations

Populations residing on and near the world’s coasts have become increasingly dependent on coastal groundwater for their supply of freshwater. Under the conditions of predicted climate changes, the expected rise in global sea level can adversely affect the quality and quantity of freshwater resources in coastal areas as a result of saltwater intrusion.

In this study, a suite of two- and three-dimensional variable-density groundwater flow models of major coastal landforms (e.g. deltas, estuaries and small islands) has been constructed to assess the effects of sea level rise (SLR), using different SLR rates of 0.5 m, 1m and 1.5 m over …

Structural Diversity In Crystal Chemistry: Rational Design Strategies Toward The Synthesis Of Functional Metal-Organic Materials, Amy J. Cairns

USF Tampa Graduate Theses and Dissertations

Metal-Organic Materials (MOMs) represent an important class of solid-state crystalline materials. Their countless attractive attributes make them uniquely suited to potentially resolve many present and future utilitarian societal challenges ranging from energy and the environment, all the way to include biology and medicine. Since the birth of coordination chemistry, the self-assembly of organic molecules with metal ions has produced a plethora of simple and complex architectures, many of which possess diverse pore and channel systems in a periodic array. In its infancy however this field was primarily fueled by burgeoning serendipitous discoveries, with no regard to a rational design approach …

Chemical Investigation Of The Antarctic Marine Invertebrates Synoicum Adareanum And Artemisina Plumosa, Jaime Heimbegner Noguez

USF Tampa Graduate Theses and Dissertations

Of the small percentage of organisms chemically investigated over the years as potential sources of natural products, much less is known about those from the marine realm. Despite the lack of attention they have received in comparison to terrestrial organisms, marine life have recently been found to represent a valuable source for novel bioactive compounds. Cold water marine habitats are home to a plethora of organisms that have the ability to produce secondary metabolites that exhibit a great deal of diversity in both their chemical structures and biological activities. The chemical investigation of these unique and relatively unstudied ecosystems is …

Statistical Learning And Behrens-Fisher Distribution Methods For Heteroscedastic Data In Microarray Analysis, Nabin K. Manandhr-Shrestha

USF Tampa Graduate Theses and Dissertations

The aim of the present study is to identify the di®erentially expressed genes be- tween two di®erent conditions and apply it in predicting the class of new samples using the microarray data. Microarray data analysis poses many challenges to the statis- ticians because of its high dimensionality and small sample size, dubbed as "small n large p problem". Microarray data has been extensively studied by many statisticians and geneticists. Generally, it is said to follow a normal distribution with equal vari- ances in two conditions, but it is not true in general. Since the number of replications is very small, …

Metal-Organic Materials: From Design Principles To Practical Applications, Mohamed H. Alkordi

USF Tampa Graduate Theses and Dissertations

The works presented herein outline rational design approaches towards construction of solid state materials with great potentials to answer some of current demanding applications. Specifically, the materials targeted are metal−organic materials with desirable structural features and functional properties. Metal−organic materials are constructed from organic linker molecules and metal ions under relatively mild solvothermal reaction conditions that preserve the structural features of the relatively simple building blocks. Therefore, it is feasible to conceive a retrospective pathway of the reaction and thus deconstruct the desirable structures into simple, chemicallyrelevant, building blocks in an approach known as the molecular building block approach. Due …

Serum Antibodies To Human Papillomavirus Type 6, 11, 16 And 18 And Their Role In The Natural History Of Hpv Infection In Men, Beibei Lu

USF Tampa Graduate Theses and Dissertations

Our understanding of humoral immune response to human papillomavirus (HPV) infection has been mainly derived from studies in women. Very little is known about humoral immune response to HPV in men. There is also a growing interest in understanding the burden of HPV exposure in the subgroups of the male population, including men who have sex with women (MSW), men who have sex with men (MSM) and men who have sex with both men and women (MSMW). This dissertation was undertaken to understand and characterize humoral immune response, measured by detectable serum antibody IgG, to HPV 6, 11, 16 and …

Magnetocaloric Effect In Thin Films And Heterostructures, Christopher Bauer

USF Tampa Graduate Theses and Dissertations

The goals of this work are the optimization of the magnetocaloric effect in Gadolinium thin film structures. We approach this issue from two directions, that of process optimization and of interface effects. Past results showed Gd₂O₃ in our Gadolinium thin films, and the presence of such oxide seemed to grow with the temperature at which the film was grown or annealed. Comparison of samples grown without chamber gettering to those that were gettered show differences in their structural and magnetic properties, and we conclude that gettering is an effective step in enhancing the quality of Gd thin …

Heat Stress Evaluation Of Protective Clothing Ensembles, Amanda Lee Pease

USF Tampa Graduate Theses and Dissertations

Clothing directly affects the level of heat stress exposure. Useful measures to express the thermal characteristics are WBGT (wet bulb globe temperature) clothing adjustment factor (CAF) or apparent total evaporative resistance (R_e,T,_a). The CAF is assigned through laboratory wear trials following a heat stress protocol in which the air temperature and humidity are progressively increased until the participant clearly loses the ability to maintain thermal equilibrium. The critical condition is the point of thermal transition and from these conditions both the CAF and Re,T,a are computed. The first objective of this study is to compare the …

Motion Analysis Of Fluid Flow In A Spinning Disk Reactor, Valentina N. Korzhova

USF Tampa Graduate Theses and Dissertations

The flow of a liquid film over a rapidly rotating horizontal disk has numerous industrial applications including pharmaceuticals, chemical engineering, bioengineering, etc. The analysis and control of complex fluid flows over a rapidly rotating horizontal disk is an important issue in the experimental fluid mechanics. The spinning disk reactor exploits the benefits of centrifugal force, which produces thin highly sheared films due to radial acceleration. The hydrodynamics of the film results in excellent fluid mixing and high heat or mass transfer rates.

This work focuses on developing a novel approach for fluid flow tracking and analysis. Specifically, the developed algorithm …

Automatic Red Tide Detection Using Modis Satellite Images, Wijian Cheng

USF Tampa Graduate Theses and Dissertations

Red tides pose a significant economic and environmental threat in the Gulf of Mexico. Detecting red tide is important for understanding this phenomenon. In this thesis, machine learning approaches based on Random Forests, Support Vector Machines and K-Nearest Neighbors have been evaluated for red tide detection from MODIS satellite images. Detection results using machine learning algorithms were compared to ship collected ground truth red tide data. This work has three major contributions. First, machine learning approaches outperformed two of the latest thresholding red tide detection algorithms based on bio-optical characterization by more than 10% in terms of F measure and …

Cost/Effectiveness Analysis Of Obtaining Operational Estimates Of Reference Evapotranspiration, Peninsular Florida, Usa, Michael G. Kittridge

USF Tampa Graduate Theses and Dissertations

The objective of this study is to conduct a cost/effectiveness analysis of the computation of reference evapotranspiration (ETo) in the peninsular of Florida. A meteorological station on the Fort Meade Mine in Polk County, Florida was used to provide data for the calculation of ETo. Five ETo equations were tested to determine the accuracy and cost/effectiveness to the fully measured ASCE Penman-Monteith (Full ASCE-PM) equation on daily, monthly, and annually time steps. The ETo equations ranged in amounts of parameters from the Full ASCE-PM to the Hargreaves. The energy terms accounted for approximately 90% of the total ETo flux. Solar …

Hydrostratigraphy And Groundwater Migration Within Surficial Deposits At The North Lakes Wetland, Hillsborough County, Florida, Jason J. Laroche

USF Tampa Graduate Theses and Dissertations

A wetland in west-central Florida was studied to characterize the local hydrostratigraphic configuration of surficial deposits overlying more-permeable limestones and conceptualize groundwater recharge. Eight continuous cores were drilled through the surficial deposits and partially into the underlying limestone. A total of 111 samples were extracted from the cores for laboratory sediment analyses and testing. The surficial deposits are roughly eight meters thick and made up of upper and lower clean-sand hydrostratigraphic layers (S1 and S3, respectively) separated by a low-permeability layer of clayey sand (S2). Also, a discontinuous low-permeability layer of clayey sand (S4) lies between S3 and the top …

Functionalization Of Resorcinarenes And Study Of Antimicrobial Activity, Kirankirti Muppalla

USF Tampa Graduate Theses and Dissertations

Cavitands are very important class of compounds in supramolecular chemistry. These molecules contain rigid enforced cavity,and have attracted considerable attention in supramolecular chemistry as building blocks for the construction of carcerands, hemicarcerands, and other host guests complexes. Nearly 40 years ago, Niederl and Vogel laid foundation for the study of such type of condensation reactions. In our laboratory we are involved in synthesis of resorcinarenes with readily available substrates such as resorcinol and aldehydes to form a cyclic tetramer.

Herein, I present detailed studies about the functionalization of the synthesized tetramers and their antimicrobial activity. Octahydroxy resorcinarenes were synthesized and …

Climatic And Structural Controls On The Geomorphology Of Wadi Sana, Highland Southern Yemen, Joshua Michael Anderson

USF Tampa Graduate Theses and Dissertations

Middle Holocene climate change forced significant environmental response and influenced human activities throughout southern Arabia. Climate models and proxy data indicate that climate along the southern Arabian peninsula changed from a moist phase, spanning the early to middle Holocene, to an arid phase, which persisted for the last ca. 5,000 years. A weakening and southward shift of the Southwest Indian Monsoon System, forced by northern hemisphere insolation variations in the precession band and/or glacial boundary conditions, is suggested as the mechanism for the abrupt shift to more arid conditions. Geoarchaeological evidence suggests that agriculture was more widespread and evolved alongside …

Evanescent Field Absorption Sensing Using Sapphire Fibers, Michael Grossman

USF Tampa Graduate Theses and Dissertations

This thesis explores the application of coiled sapphire multimode optical fibers for evanescent wave chemical sensing in both the visible spectrum and the near infrared. As has been suggested in the literature pertaining to silica fibers, bending converts low-order modes to high order ones, which leads to more evanescent absorption and thus a more sensitive chemical detector. By coiling the fiber many times, it was expected that even greater sensitivity would be attained.

Experiments were performed to investigate the sensor response to different solutions and to characterize this response. In the first of three experiments, the large absorption peak of …

Influence Of Evapotranspiration On Patterns Of Ground-Water Conductivity In Small Basins, Ana Jiménez

USF Tampa Graduate Theses and Dissertations

Ground-water conductivity data were obtained from shallow wells in a 12 km2 stream-basin along a 400 m transect, extending from the divide to the stream. The stream, Pringle Branch, is a second-order perennial stream in Hillsborough County, Florida. The shallow stratigraphy consists of 2-3 m of fine sand over a layer of clayey silt and silty clay. Vegetation cover includes grasses on the upper and middle slope, and riparian woodlands on the foot slope and floodplain. Precipitation is about 1.3 m per year. Shallow ground-water conductivity is about 50 uS/cm at the divide. It increases moderately along the mid slope, …

Geophysical Investigations And Groundwater Modeling Of The Hydrologic Conditions At Masaya Caldera, Nicaragua, Richard Eric Macneil

USF Tampa Graduate Theses and Dissertations

Masaya volcano, Nicaragua, has been the site of tremendous Plinian basaltic eruptions. Two eruptions ~6,500 and 2,250 BP formed the 6 kilometer (km) x 11 km, northwest trending Masaya caldera. The present day active Santiago Crater within the caldera is the site of persistent volcano degassing and occasional phreatic explosions. While the mechanism responsible for these phreatic explosions is unclear, one possible explanation is the interaction of groundwater with the shallow magma chamber beneath Masaya. This interaction with meteoric water is supported by the substantial steam discharge from the vent, which is significantly larger than other similar volcanoes in the …

An Investigation Into The Antifungal Activities Of N-Thiolated Beta- Lactams Against Selected Candida Species, Marci Culbreath

USF Tampa Graduate Theses and Dissertations

β-lactam antibiotics have long been a reliable course of treatment for bacterial infections. However, with recent increases in resistance and rising populations of immunocompromised patients new β-lactams have been synthesized and tested. The Turos laboratory has recently discovered novel β-lactams that have a mode of action distinct from penicillin and other β-lactam antibiotics as cell lysis is not observed. In the current investigations, these compounds are shown to also have antifungal properties. The rising incidence and prevalence of invasive fungal infections has become an increasing concern. The most common fungal pathogens involved in these infections are species in the genus …

Impacts Of Rainfall Events On Wastewater Treamtent Processes, Erin K. Mcmahan

USF Tampa Graduate Theses and Dissertations

Current research is revealing that stormwater can carry pathogens and that this stormwater is entering wastewater treatment facilities. During periods of intense rainfall, not only can stormwater carry higher amounts of pathogens, but it also increases the flow rate to the wastewater treatment facility. In many instances, the flow rate exceeds the facilities' treatment capacity and can impact treatment performance. The purpose of this study was to identify whether wastewater treatment is impaired during periods of increased rainfall, and to compare current policies that address this issue. The study was conducted using a case study approach to analyze historical precipitation …

Chemo-Enzymatic Route To Synthesis Of Biodegradable Polymers And Glycolipid Analogs, Surbhi Bhatt

USF Tampa Graduate Theses and Dissertations

New catalytic synthetic methods in organic chemistry that satisfy increasingly stringent environmental constraints are in great demand by the pharmaceutical and chemical industries. Studies over last 15 years have revealed that activity of enzymes can be increased in organic solvents rather than their natural aqueous environment. Because of their ease of use, high selectivity and environment friendliness, enzymes are enjoying increasing popularity in today's synthesis world.

Chapter 1 describes chemo-enzymatic synthesis of various glycolipid analogs. A highly regioselective macrolactonization was achieved using lipase from Candida antarctica as a catalyst. It also describes evaluation of lipases from different source and their …

Evaluation Of Geophysical And Thermal Methods For Detecting Submarine Groundwater Discharge (Sgd) In The Suwannee River Estuary, Florida, Matthew Weiss

USF Tampa Graduate Theses and Dissertations

Submarine groundwater discharge (SGD) represents a significant portion of the total discharge from coastal aquifers through diffuse seepage and point source springs, but can be difficult to locate. SGD is important as it can be a source of nutrients to estuaries and other coastal ecosystems. In an effort to evaluate geophysical and thermal methods for detecting SGD on the Florida Gulf coast, a suite of water-borne surveys were run in conjunction with aerial thermal imagery over the lower Suwannee River and estuary in March and September 2005. Thermal imagery exploits temperature differences between discharging groundwater and surface water. Thermal images …

Dynamic Monitoring Of Cytotoxicity Using Electric Cell Substrate Impendence Sensing, Alfred Brian Wafula

USF Tampa Graduate Theses and Dissertations

Electric cell-substrate impedance sensing (ECIS) pioneered by Giaever and Keese is suitable for continuous, automatic and real-time cell attachment analysis. ECIS is a novel electrical method to study, in real time, many of the activities of animal cells when grown in tissue culture. These include morphological changes, cell locomotion, and other behaviors directed by the cell's cytoskeleton. One of the most direct ECIS measurements is that of the attachment and spreading behaviors of cells. These measurements allow one to study and quantify the interaction of cultured cells with extracellular matrix (ECM) proteins and other macromolecules continuously and in real time. …

Coastal Processes And Anthropogenic Factors Influencing The Geomorphic Evolution Of Weedon Island, Florida, Jeanne Lambert

USF Tampa Graduate Theses and Dissertations

Weedon Island, a peninsula located on the western inner shoreline of Tampa Bay, Florida, is the location of a collaborative geological and archaeological project that aims to relate the present day geomorphology to natural processes and human occupational activity during the middle to late Holocene. The area is known for extensive archaeological sites, which were originally investigated in the 1920s, although they have received relatively little scientific attention during most of the last century. We hypothesize that activities associated with pre-historic human occupation of Weedon Island at various times during the last ca. 5,000 years influenced the geomorphic evolution of …