Physical Sciences and Mathematics Commons^™

Characterization Of The N-Terminal Domains And Disease-Causing Mutations Of The Human Wilson Protein, Joshua Mutambuki Muia

Dissertations

The Wilson protein (ATP7B) is a copper transporting ATPase that is involved in copper trafficking and homeostasis. Unlike the other known P-type ATPases, it possesses six homologous metal binding domains at the N-terminal end. Several mutations in the gene coding for this protein lead to Wilson disease, a hepatic disorder characterized by impaired excretion of copper in the bile, and accumulation of copper in body organs such as the liver, brain, kidney, and eye cornea. Characterization of various regions of expressed and purified ATP7B has been hampered by its low stability, aggregation and degradation.

In this research, novel methods were …

The Lipid Acyl-Chain Dynamics In Giant Liposomes And Characterization Of Domain 4 Of The Wilson Disease Protein, Wilson Okumu

Dissertations

Steady-state and nanosecond time-resolved fluorescence of the nitro-2, 1, 3- benzoxadiazol-4-yl (NBD) probe and line tension force were determined in phospholipids acyl-chain dynamics in giant liposomes made from a homologous series of phosphatidylcholines (PC). The fluorescence spectroscopy of a NBD probe attached to the headgroup (NBD PE) or the tail (NBD PC) of the phospholipid were used to determine the rate of dithionite quenching in a homologous series of phospholipids. Similar experiments were performed in the gel and the fluid phases of the l,2-dimyristoyl-s«-glycero-3-phosphocholine (DMPC ). Nanosecond time scale lifetimes and anisotropy measurements were obtained by a 470 nm LED …

Designing Molecular And Nanoscale Materials For Environmental Chemistry Processes, Wen Guo

Dissertations

This dissertation focuses on fundamental studies to identify materials that detect and degrade common organic environmental pollutants. Chapter 1 represents the overview of two widespread ground water contaminants: organohalides and organophosphorous compounds. Due to continuous usage of these compounds as well as their toxicity, reliable and sensitive methods for their detection and degradation are urgently needed. In Chapter 2 a description of molecular sensors designed with high sensitivity and selectivity to detect and distinguish between three organophosphorus (OP) pesticides are described. These sensors provide dual optical and electrochemical signals for detection, which minimizes false-positives. The signal transduction occurs in real …

The In-Situ Removal Of Menthofuran From Peppermint Oil And Subsequent Reactions Of The Solid-Supported Adduct, Robert Joseph Justice Ii

Dissertations

Menthofuran is an aromatic heterocyclic compound found naturally in the essential oil of Mentha piperita L. or peppermint. Natural peppermint oil may contain between approximately 2 to 8% menthofuran.

Peppermint oil is a complex mixture of over 350 identified chemical compounds; market demands dictate the extent of processing the oil undergoes prior to being introduced into consumer goods. Menthofuran has long been known to readily autoxidize, either alone or within the peppermint oil matrix, leading to a discolored oil with negative organoleptic consequences that adversely affects its commercial viability. Therefore, a facile method for selective removal of menthofuran was developed …

Perturbed Spherical Objects In Acoustic And Fluid Flow Fields, Manmeet Kaur

Dissertations

In this study, the time averaged acoustic radiation force and drag on a small, nearly spherical object suspended freely in a stationary sound wave field in a compressible, low viscosity fluid is to be calculated. This problem has been solved for a spherical object, and it has many important engineering applications related to segregation and separation processes for particles in fluids such as water. Small but significant errors have occurred in the predicted behavior of the particles using the existing approximate solutions based on perfect spheres. The classical approach has been extended in this research to objects that deviate slightly …

Algorithms In Comparative Genomics, Satish Chikkagoudar

Dissertations

The field of comparative genomics is abundant with problems of interest to computer scientists. In this thesis, the author presents solutions to three contemporary problems: obtaining better alignments for phylogeny reconstruction, identifying related RNA sequences in genomes, and ranking Single Nucleotide Polymorphisms (SNPs) in genome-wide association studies (GWAS).

Sequence alignment is a basic and widely used task in bioinformatics. Its applications include identifying protein structure, RNAs and transcription factor binding sites in genomes, and phylogeny reconstruction. Phylogenetic descriptions depend not only on the employed reconstruction technique, but also on the underlying sequence alignment. The author has studied and established a …

Understanding Cognitive Differences In Processing Competing Visualizations Of Complex Systems, Madhavi Mukul Chakrabarty

Dissertations

Node-link diagrams are used represent systems having different elements and relationships among the elements. Representing the systems using visualizations like node-link diagrams provides cognitive aid to individuals in understanding the system and effectively managing these systems. Using appropriate visual tools aids in task completion by reducing the cognitive load of individuals in understanding the problems and solving them. However, the visualizations that are currently developed lack any cognitive processing based evaluation. Most of the evaluations (if any) are based on the result of tasks performed using these visualizations. Therefore, the evaluations do not provide any perspective from the point of …

Integration Of Electronic And Optical Techniques In The Design And Fabrication Of Pressure Sensors, Ivan Padron

Dissertations

Since the introduction of micro-electro-mechanical systems fabrication methods, piezoresistive pressure sensors have become the more popular pressure transducers. They dominate pressure sensor commercialization due to their high performance, stability and repeatability. However, increasing demand for harsh environment sensing devices has made sensors based on Fabry-Perot interferometry the more promising optical pressure sensors due to their high degree of sensitivity, small size, high temperature performance, versatility, and improved immunity to environmental noise and interference. The work presented in this dissertation comprises the design, fabrication, and testing of sensors that fuse these two pressure sensing technologies into one integrated unit. A key …

Nonlinear Evolution Of Annular Layers And Liquid Threads In Electric Fields, Qiming Wang

Dissertations

The nonlinear dynamics of viscous perfectly conducting liquid jets or threads under the action of a radial electric field are studied theoretically and numerically here. The field is generated by a potential difference between the jet surface and a concentrically placed electrode of given radius. A long-wave nonlinear model that is used to predict the dynamics of the system and in particular to address the effect of the radial electric field on jet breakup is developed, Two canonical regimes are identified that depend on the size of the gap between the outer electrode and the unperturbed jet surface. For relatively …

Relationship Between Photospheric Magnetic Fields And Coronal Activities, Changyi Tan

Dissertations

Coronal emission comes in two forms, a steady component where the corona is heated to million degrees and a much hotter transient component of solar flares. Both components are known to be related to the evolution of surface magnetic fields. This dissertation studies the evolution of photospheric magnetic fields and flow fields and their relation to the properties of these two coronal emission components.

The key issue in the study of the steady coronal emission is the coronal heating problem: how the corona is heated to millions of degrees while the underlying solar photosphere is only a few thousand degrees. …

Job Seeking And Job Application In Social Networking Sites : Predicting Job Seekers' Behavioral Intentions, Maria Marcella Plummer

Dissertations

Social networking sites (SNSs) are revolutionizing the way in which employers and job seekers connect and interact with each other. Despite the reported benefits of SNSs with respect to finding a job, there are issues such as privacy concerns that might be deterring job seekers from using these sites in their attempts to secure a job. It is therefore important to understand the factors that are salient in predicting job seekers' use of SNSs in applying for jobs.

In this research, a theoretical model was developed to explicate job seekers' intentions to use SNSs to apply for jobs. Two aspects …

Semantics And Efficient Evaluation Of Partial Tree-Pattern Queries On Xml, Xiaoying Wu

Dissertations

Current applications export and exchange XML data on the web. Usually, XML data are queried using keyword queries or using the standard structured query language XQuery the core of which consists of the navigational query language XPath. In this context, one major challenge is the querying of the data when the structure of the data sources is complex or not fully known to the user. Another challenge is the integration of multiple data sources that export data with structural differences and irregularities. In this dissertation, a query language for XML called Partial Tree-Pattern Query (PTPQ) language is considered. PTPQs generalize …

Institutional Controls And Brownfield Redevelopment, Ronnachai Tiyarattanachai

Dissertations

Institutional controls (ICs) are used to reduce risks to human health and environment from exposure to contaminants at Brownfield sites. Regulators approve use of ICs in Brownfield redevelopment with the expectation that ICs will remain effective over the long-term under proper oversight mechanisms. However, a recent review of compliance statuses of Brownfield sites with ICs implemented in Massachusetts and New Jersey indicated a significant percentage of the sites are out of compliance status.

Implementation of ICs depends on compliance efforts by humans. Thus, consistency of implementation over the long-term is questionable because there are several potential problems due to human …

Modeling And Quasi-Monte Carlo Simulation Of Risk In Credit Portfolios, Bo Ren

Dissertations

Credit risk is the risk of losing contractually obligated cash flows promised by a counterparty such as a corporation, financial institution, or government due to default on its debt obligations. The need for accurate pricing and hedging of complex credit derivatives and for active management of large credit portfolios calls for an accurate assessment of the risk inherent in the underlying credit portfolios. An important challenge for modeling a credit portfolio is to capture the correlations within the credit portfolio. For very large and homogeneous portfolios, analytic and semi-analytic approaches can be used to derive limiting distributions. However, for portfolios …

Virtual World Commerce Adoption (Vwca) : A Case Study Of Second Life Investigating The Impacts Of Perceived Affordances, Trust, And Need Satisfaction, Kamolbhan Olapiriyakul

Dissertations

Virtual worlds are computer-simulated worlds in which multi-players can simultaneously interact in a rich graphical environment. The development of virtual worlds, along with the massive growth of users, creates opportunities for business organizations. This dissertation involves many studies regarding virtual world adoption in business by virtual consumers.

Most of the research in Information Systems (IS) was conducted investigating factors influencing technology adoption, such as ease of use and usefulness, subjective norms and behavioral controls, self-efficacy, performance and effort expectancy, flow, etc. However, most of these research studies focused neither on design aspects related to affordances nor users' goal-oriented behaviors, such …

Investigation Of Network Architecture Development And Properties In Thermoset Matrices, Jeremy Owen Swanson

Dissertations

Matrices employed in composite materials directly influence overall composite properties. In all thermoset materials, molecular level interactions and transformations during cure result in heterogeneous architecture. Variability in connectivity results from the often dramatic spatial and topological changes that occur during the crosslinking process. Compatibility (fillers, pigments, additives), temperature gradients and reactivity differences in the precursors only serve to increase the complexity of network formation. The objective of the research herein is to characterize and understand the relationships between cure conditions, conversion, connectivity, network architecture and properties in glassy thermosetting matrix resins.

In this research, epoxy and vinyl ester resins (VERs) …

Investigating Fabrication Methods For Micro Single-Chamber Solid Oxide Fuel Cells, Man Yang

Dissertations

A Solid Oxide Fuel Cell (SOFC) is a green energy technology that offers a cleaner and efficient alternative to fossil fuels. The efficiency and utility of SOFCs can be enhanced by fabricating miniaturized component structures within the fuel cell footprint. This research focuses on the microfabrication of parallel-connected inter-digitized design of micro single chamber SOFC (μ-SC-SOFCs). In this dissertation, candidate micro/nano fabrication techniques to precisely fabricate micro patterns on planar substrates have been studied. These include EBL, soft lithography, photolithography, and direct-writing. After analyzing preliminary results, two combinational approaches were further investigated. The first method involves the combination of the …

A Comparison Of Computer-Based Training And Traditional Classroom Training For The Sad Corporation Job Placement Team, Janice Brown Adams

Dissertations

Purpose: The purpose of this research study was to compare the effectiveness of computer-based training with traditional classroom training; both instructional delivery methods utilized the traditional classroom curriculum as a means of delivery for an on-the-job company-sponsored program. The JAW-SAD Human Resource Center (HRC) is a training facility for the SAD Corporation, a major international manufacturing company. The HRC is responsible for conducting continuous training and certification of SAD employees. Historically, training has been provided through conference workshops with a traditional classroom training format. The motivation and importance for this study was influenced by an administrative requirement to determine the …

Mastery Of Sixth-Grade Mathematics Expectations As Measured By The Seventh-Grade Michigan Education Assessment Program From 2005 To 2007, Marian Prince

Dissertations

Purpose

The purpose of this study is to document sixth-grade mathematics mastery as measured by the seventh-grade Michigan Education Assessment Program (MEAP) over a period of 3 years: 2005, 2006, and 2007. This study investigated whether mathematics performance in Michigan is related to ethnicity by analyzing student responses on the seventh-grade MEAP which evaluates students’ mastery of the Michigan sixth-grade mathematics expectations.

Method

Data from the Michigan Department of Education (MDE) containing the student scores of individual test items on the mathematics Michigan Education Assessment Program (MEAP) for seventh-grade students in Michigan for 2005 to 2007 formed the basis for …