Physical Sciences and Mathematics Commons^™

Evaluation Of The Potential Of Raman Microspectroscopy For Prediction Of Chemotherapeutic Response To Cisplatin In Lung Adenocarcinoma, Haq Nawaz, Franck Bonnier, Peter Knief, Orla Howe, Fiona Lyng, Aidan Meade, Hugh Byrne

Articles

The study of the interaction of anticancer drugs with mammalian cells in vitro is important to elucidate the mechanisms of action of the drug on its biological targets. In this context, Raman spectroscopy is a potential candidate for high throughput, noninvasive analysis. To explore this potential, the interaction of cis-Diamminedichloroplatinum (II) (Cisplatin) with a human lung adenocarcinoma cell line (A549) was investigated using Raman microspectroscopy. The results were correlated with parallel measurements from the MTT cytotoxicity assay, which yielded an IC50 value of 1.2±0.2 μM. To further confirm the spectral results, Raman spectra were also acquired from DNA extracted from …

Investigation Of The Influence Of High Risk Human Papillomavirus On The Biochemical Composition Of Cervical Cancer Cells Using Vibrational Spectroscopy, Kamila Ostrowska, Alison Malkin, Aidan Meade, John O'Leary, Cara Martin, Cathy Spillane, Hugh Byrne, Fiona Lyng

Articles

The main aetiology of cervical cancer is infection with high risk human papillomavirus (HPV). Cervical cancer is almost 100% curable if detected in the early stages. Thus, information about the presence and levels of HPV in patient samples has high clinical value. As current screening methods, such as the Pap smear test, are highly subjective and in many cases show low sensitivity and specificity, new supportive techniques are desirable to improve the quality of cervical cancer screening. In this study, vibrational spectroscopic techniques (Raman and Fourier Transform Infra Red absorption) have been applied to the investigation of four cervical cancer …

Silver Nanoparticle Polymer Composite Based Humidity Sensor, Aoife Power, Tony Betts, John Cassidy

Articles

Silver nanoparticles were synthesised by a chemical reduction process in order to produce an aqueous colloidal dispersion. The resulting colloids were then characterised by a combination of UV-Vis spectroscopy, dynamic light scattering, X-ray diffraction and transmission electron microscopy and the nanoparticles were found to have an average diameter of 20–22 nm. The Ag/polymer nanocomposites were then applied to platinum interdigital electrodes as sensor coatings and the capability of the resulting sensor as a humidity detector investigated. With the application of 1 V, a current developed which was found to be directly proportional to humidity levels. The sensor gives a reversible, …

Motion In Augmented Reality Games: An Engine For Creating Plausible Physical Interactions In Augmented Reality Games, Brian Mac Namee, David Beaney, Qingqing Dong

Articles

The next generation of Augmented Reality (AR) games will require real and virtual objects to coexist in motion in immersive game environments. This will require the illusion that real and virtual objects interact physically together in a plausible way. The Motion in Augmented Reality Games (MARG) engine described in this paper has been developed to allow these kinds of game environments. The paper describes the design and implementation of the MARG engine and presents two proof-of-concept AR games that have been developed using it. Evaluations of these games have been performed and are presented to show that the MARG engine …

Assessing The Application Of 3d Collaborative Interfaces Within An Immersive Virtual University, Gavin Mcardle, Bianca Schoen-Phelan, Michela Bortolotto

Articles

The need to stimulate and engage students is of paramount importance within any learning scenario. Despite this, recent developments in online learning have failed to take this requirement into account. As a result e-learning courses which utilise traditional online learning management systems have a higher dropout rate than their classroom based counterparts. The attrition rate is attributed to boredom with the interfaces used to deliver learning material and also to the lack of opportunities to interact socially with others. Furthermore, being in a virtual environment imposes a whole new set of challenges onto users due to the distinct lack of …

Enhancing 3d Lidar Data Handling, Debra Laefer, Michela Bertolotto, Bianca Schoen-Phelan

Articles

more evident than in the limited capabilities of Spatial Information Systems (SIS) to manipulate data from aerial Light Detection and Ranging (LiDAR). Currently, multiple software packages are needed to conduct three-dimensional (3D) analysis of LiDAR data. This workflow heavily relies on importing and exporting different file formats, thus potentially losing accuracy and increasing costs. Ideally, all required functionality would be available within a single system. New functionalities in Oracle Spatial combined with our enhanced 3D-indexing for hosting and analysing offer a critical breakthrough in this area.

On The Socles Of Characteristic Subgroups Of Abelian P-Groups, Brendan Goldsmith, Peter Danchev

Articles

Fully invariant subgroups of an Abelian p-group have been the object of a good deal of study, while characteristic subgroups have received somewhat less attention. Recently the socles of fully invariant subgroups have been studied and this led to the notion of a socle-regular group. The present work replaces the fully invariant subgroups with characteristic ones and leads in a natural way to the notion of a strongly socle-regular group. A surprising relationship, mirroring that between transitive and fully transitive groups, is obtained.

From Permutahedron To Aassociahedron, Colum Watt, Thomas Brady

Articles

For each finite real reflection group $W$, we identify a copy of the type-$W$ simplicial generalised associahedron inside the corresponding simplicial permutahedron. This defines a bijection between the facets of the generalised associahedron and the elements of the type $W$ non-crossing partition lattice which is more tractable than previous such bijections. We show that the simplicial fan determined by this associahedron coincides with the Cambrian fan for $W$.

The Similarity Problem For Indefinite Sturm-Liouville Operators With Periodic Coefficients, Aleksey Kostenko

Articles

We investigate the problem of similarity to a self-adjoint operator for $J$-positive Sturm-Liouville operators $L=\frac{1}{\omega}(-\frac{d^2}{dx^2}+q)$ with $2\pi$-periodic coefficients $q$ and $\omega$. It is shown that if 0 is a critical point of the operator $L$, then it is a singular critical point. This gives us a new class of $J$-positive differential operators with the singular critical point 0. Also, we extend the Beals and Parfenov regularity conditions for the critical point $\infty$ to the case of operators with periodic coefficients.

Fractional Anisotropic Diffusion For Noise Reduction In Magnetic Resonance Images, Jonathan Blackledge, Matthew Blackledge

Articles

We extend the method of anisotropic diffusion for noise reduction in digital images to the case when the diffusion processes are non-Gaussian and Levy distributed. This yields a fractional diffusion equation characterised by the Levy index. A solution to this equation is considered and a numerical algorithm developed. The algorithm is then applied as a case study to the problem of reducing noise in magnetic resonance imaging. The focus of this study is on diffusion weighted images which have low signal-to-noise ratios.

Rapid Microwave Synthesis Of Mesoporous Tio2 For Electrochromic Displays, Suresh Pillai, Declan Mccormack, Pradeepan Periyat, Nigel Leyland, David Corr, John Colreavy

Articles

No abstract provided.

From Permutahedron To Associahedron, Colum Watt, Thomas Brady

Articles

For each finite real reflection group $W$, we identify a copy of the type-$W$ simplicial generalised associahedron inside the corresponding simplicial permutahedron. This defines a bijection between the facets of the generalised associahedron and the elements of the type-$W$ non-crossing partition lattice which is more tractable than previous such bijections. We show that the simplicial fan determined by this associahedron coincides with the Cambrian fan for $W$.

Climbing Eements In Finite Coxeter Groups, Colum Watt, Thomas Brady, Aisling Kenny

Articles

We define the notion of a climbing element in a finite real reflection group relative to a total order on the reflection set and we characterise these elements in the case where the total order arises from a bipartite Coxeter element.

Multi–Component Nls Models On Symmetric Spaces: Spectral Properties Versus Representations Theory, Vladimir Gerdjikov, Georgi Grahovski

Articles

The algebraic structure and the spectral properties of a special class of multicomponent NLS equations, related to the symmetric spaces of BD.I-type are analyzed. The focus of the study is on the spectral theory of the associated Lax operator to these nonlinear evolutionary equations for different fundamental representations of the underlying simple Lie algebra g. Special attention is paid to the spinor representation of the orthogonal Lie algebras of B type.

Schrödinger Operators With Δ'Interactions And The Krein–Stieltjes String, Aleksey Kostenko, M. M. Malamud

Articles

We investigate one dimensional symmetric Schrödinger operator HX, β with δ' interactions of strength β = ⊂ on a discrete set X = ⊂ [0, b), b ≤ +∞ (xn ↑ b). We consider HX, β as an extension of the minimal operator Hmin := –d2/dx2 (\X) and study its spectral properties in the frame work of the extension theory by using the technique of boundary triplets and the corresponding Weyl functions. The construction of a boundary triplet for is given in the case d∗ := infn ∈ |xn – xn – 1| = 0. We show that spectral properties …

The Antioxidant Properties Of Whey Permeate Treated Fresh-Cut Tomatoes, Lubna Ahmed, Ana Belen Martin-Diana, Daniel Rico, Catherine Barry-Ryan

Articles

The aim of this research was to analyse the effects of three types of cheese whey permeate treatment on the antioxidant properties of fresh-cut tomatoes. Tomatoes were treated with whey permeate concentrate (PC), delactosed permeate (DP) and delactosed concentrate (DC), stored at 4 C for 10 days and compared to samples treated with the industry standard, chlorine (120 ppm). Samples treated with DP retained significantly higher antioxidant activity (FRAP) and total phenols (TP), when compared with those treated with PC and DC. DP showed significantly higher results than chlorine for DPPH, FRAP and TP. In DPPH assay, all whey permeate-treated …

Serine- And Arginine-Rich Proteins 55 And 75 (Srp55 And Srp75) Induce Production Of Hiv-1 Vpr Mrna By Inhibiting The 5′-Splice Site Of Exon 3, Anna Tranell, Eva Maria Fenyö, Stefan Schwartz

Articles

HIV-1 non-coding exon 3 can either be spliced to exons 4, 4a, 4b, 4c, and 5 to generate tat, rev, and nef mRNAs or remain unspliced to produce the 13a7 vpr mRNA. Here we show that serine- and arginine-rich proteins 55 and 75 (SRp55 and SRp75) inhibit splicing from the 5′-splice site of exon 3 thereby causing an accumulation of the partially unspliced 13a7 vpr mRNA. In contrast, serine- and arginine-rich protein 40 (SRp40) induces splicing from exon 3 to exon 4, thereby promoting the production of the 1347 tat mRNA. We demonstrate that SRp55 stimulates vpr mRNA production by …

Heat Transfer Through The Boundary Layer On A Moving Cylindrical Fibre, Brendan Redmond, David Mcdonnell

Articles

This paper applies boundary layer theory to the process of manufacturing polymer fibres known as the melt spinning process. The rate of heat loss of the fibre during this process, characterised by the local Nusselt number, is evaluated by means of a Pohlhausen integral method.

Mobile Mapping System Lidar Data Framework, P. Lewis, C. Mcelhinney, Bianca Schoen-Phelan, T. Mccarthy

Articles

Mobile Mapping Systems (MMSs) for infrastructural monitoring and mapping arebecoming more prevalent as the availability and affordability of solutions that generate high accuracy geospatial data has matured.However, no existent methodology or systemexists where all theLiDAR, video, navigation,infrared and multispectraldata sources,collected from thismobile platform are integrated into a single, comprehensivedata management solution. Based on empirical experience there is a need for an MMS-data management framework where these types of data can be dynamically accessed and integratedto enable different projects with varying objectives todynamically accessdifferent MMS-data for, in one example, use in feature extraction algorithms. In this paper we introduce the LiDAR …

Vibration Phase Measurements Using Holographic Optical Elements, Viswanath Bavigadda, Raghavendra Jallapuram, Vincent Toal, Emilia Mihaylova

Articles

The application of an out-of-plane sensitive electronic speckle pattern interferometer (ESPI) using holographic optical element (HOE) to vibration amplitude and phase mapping is reported. The novelty of the proposed system is the use of a speckle reference wave stored in a reflection holographic optical element (HOE). The incorporation of a HOE minimizes the alignment difficulties. The HOE based ESPI system is compact containing only a diode laser, HOE and a digital CMOS camera. The measurement technique is a combination of time averaged ESPI and reference beam phase modulation in an unbalanced interferometer. The reference beam phase modulation is implemented by …

Electrochemical Characterisation Of Poly Arylene Vinylenes, Jonathan Moghal, Patrick Lynch, Mary Mcnamara, Hugh Byrne, Gordon Chambers

Articles

The electrochemical properties of a newly synthesised set of PPV derivatives were measured by cyclic voltammetry. A new octyloxy substituted PPV derivative was first synthesised (POPV). The structure of the POPV is varied systematically by the replacement of the phenyl units by naphthyl and anthryl units and the effect of these variations on the electrochemical properties is explored. The HOMO–LOMO levels of the polymers were determined from the onset potentials for the p-doping and n-doping scans. From this the electrochemical bandgap for each of the polymers was calculated. The systematic change in the structure of the POPV results in a …

Two-Way Diffusion Model For Short Exposure Holographic Grating Formation In Acrylamide-Based Photopolymer, Tzvetanka Babeva, Izabela Naydenova, Dana Mackey, Suzanne Martin, Vincent Toal

Articles

A theoretical model for formation of a short exposure holographic grating is presented. The model accounts for both monomer and polymer diffusion and distinguishes between short polymer chains capable of diffusing and long polymer chains that are immobile. It is shown that the experimentally observed decrease of diffraction efficiency at higher spatial frequency can be predicted by assuming diffusion of short-chain polymers away from the bright fringes. The time evolution of the refractive index modulation after short exposure is calculated and compared with experimental results. The effects of diffusion coefficients, polymerization rates, intensity and spatial frequency of recording on the …

Optimisation Of An Acrylamide-Based Photopolymer System For Holographic Inscription Of Surface Patterns With Sub-Micron Resolution, Kevin Trainer, Kevin Wearen, Dimana Nazarova, Izabela Naydenova, Vincent Toal

Articles

We describe the optimisation of the holographic patterning of sub-micrometer surface relief structures in an acrylamide-based photopolymer. A substantial improvement in the photoinduced surface relief resolution was achieved by altering the photopolymer chemical composition and by introducing a single step post recording thermal treatment of the layers. It was observed that, by optimisation of the chemical composition of the photopolymer layers, the maximum achievable spatial frequency increases from 200 l/mm to 550 l/mm. The improvement of the surface relief amplitude by alteration of the chemical composition is limited due to the fact that both decrease of the plasticiser and increase …

Photopolymerizable Nanocomposites For Holographic Recording And Sensor Application, Elsa Leite, Izabela Naydenova, Svetlana Mintova, Louis Leclercq, Vincent Toal

Articles

Novel nanocomposites consisting of a water soluble acrylamide–based photopolymer and colloidal zeolite nanoparticles of zeolite Beta and zeolite A were prepared. The interactions between the photopolymer components and zeolite nanoparticles in the photopolymerizable nanocomposites were characterized for the first time by 13C NMR and Visible spectroscopy. It was found that the zeolite Beta nanoparticles (up to 5% wt.) behave as a non-inert additive, resulting in an effective increase in layer thickness that causes doubling of the diffraction efficiency of the nanocomposite in comparison to that of the undoped photopolymer. On the other hand, the nanocomposite containing zeolite A nanoparticles showed …

Optical Properties Of Photopolymer Layers Doped With Aluminophosphate Nanocrystals, Elsa Leite, Tzvetanka Babeva, E Ng, Vincent Toal, Svetlana Mintova, Izabela Naydenova

Articles

The optical properties of photopolymer layers consisting of an acrylamide–based matrix and microporous aluminophosphate nanocrystals of AEI- type are investigated. The compatibility of the photopolymer doped with the nanoparticles is studied. The surface and volume properties of the layers with different levels of doping with microporous nanocrystals are characterized. The effective refractive indices and absorption coefficients of the doped photopolymer layers are determined and used to calculate the refractive index and porosity of pure AEI nanoparticles used as dopants. Volume transmission gratings were recorded in the doped photopolymer layers at different spatial frequencies. By spatial monitoring of the characteristic Raman …

Study Of The Photoinduced Surface Relief Modulation In Photopolymers Caused By Illumination With A Gaussian Beam Of Light, Tzvetanka Babeva, Dana Mackey, Suzanne Martin, Izabela Naydenova, Vincent Toal

Articles

The formation of surface relief profile in photopolymerisable systems when illuminated with a focused beam of light is simulated numerically using a two-way diffusion model that takes account both for monomer and short polymer chains diffusion. The concentration and spatial distribution dynamics of monomer and short and long polymer chains are calculated. The surface profile is obtained from calculated components concentrations considering different densities of monomer and polymer. The influence of the illumination time, intensity and spot diameter on the surface profiles dynamics is discussed. A good agreement between the calculated and the experimentally measured profiles is observed thus demonstrating …

Functional And Pathological Analysis Of Biological Systems Using Vibrational Spectroscopy With Chemometric And Heuristic Approaches, Aidan Meade, Colin Clarke, Franck Bonnier, Kelvin W. Poon, Amaya Garcia, Peter Knief, Kamila Ostrowska, Lorenzo Salford, Haq Nawaz, Fiona Lyng, Hugh Byrne

Articles

Vibrational spectroscopy (Raman and FTIR microspectroscopy) is an attractive modality for the analysis of biological samples since it provides a complete non-invasive acquisition of the biochemical fingerprint of the sample. Studies in our laboratory have applied vibrational spectroscopy to the analysis of biological function in response to external agents (chemotherapeutic drugs, ionising radiation, nanoparticles), together with studies of the pathology of tissue (skin and cervix) in health and disease. Genetic algorithms have been used to optimize spectral treatments in tandem with the analysis of the data (using generalized regression neural networks (GRNN), artificial neural networks (ANN), partial least squares modelling …

Spectroscopic And Chemometric Approaches To Radiobiological Analyses, Aidan Meade, Hugh Byrne, Fiona Lyng

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No abstract provided.

Studies Of Chemical Fixation Effects In Human Cell Lines Using Raman Microspectroscopy, Aidan Meade, Colin Clarke, Florence Draux, Ganesh Sockalingum, Michel Manfait, Fiona Lyng, Hugh Byrne

Articles

The in vitro study of cellular species using Raman spectroscopy has proven a powerful non-invasive modality for the analysis of cell constituents and processes. This work uses micro-Raman spectroscopy to study the chemical fixation mechanism in three human cell lines (normal skin, normal bronchial epithelium, and lung adenocarcinoma) employing fixatives that preferentially preserve proteins (formalin), and nucleic acids (Carnoy’s fixative and methanol–acetic acid). Spectral differences between the mean live cell spectra and fixed cell spectra together with principal components analysis (PCA), and clustering techniques were used to analyse and interpret the spectral changes. The results indicate that fixation in formalin …

Corrosion Protection Properties Of Various Ligand Modified Organic Inorganic Hybrid Coating On Aa 2024-T3, P.C. Rajath Varma, John Cassidy, Mohamed Oubaha, Colette Mcdonagh, John Colreavy, Brendan Duffy

Articles

The inclusion of zirconium precursors to prepare organosilane solgel coatings improves the corrosion protection performance of the coatings on aluminium and steel. The inherent differences in the hydrolysis rates of the silane and zirconium precursors, various ligands were used to control the hydrolysis by decreasing the number of reactive alkoxide group. Hybrid sols were synthesised using 3-(trimethoxysilyl) propylmethacrylate (MAPTMS) and zirconium n-propoxide chelated with organic ligands including different organic acids, acetyl acetone and 2 2’ bipyridyl. The effects of zirconia inclusion on the properties of the coatings were compared on the aerospace alloy AA 2024-T3.

Electrochemical analysis and salt spray …