Open Access. Powered by Scholars. Published by Universities.®

Physical Sciences and Mathematics Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 6 of 6

Full-Text Articles in Physical Sciences and Mathematics

Effects Of Elevated Temperature On 8-Hydroxy-2'-Deoxyguanosine Expression, Dna Damage And Cellular Apoptosis In The American Oyster (Crassostrea Virginica), Md Faizur Rahman Dec 2020

Effects Of Elevated Temperature On 8-Hydroxy-2'-Deoxyguanosine Expression, Dna Damage And Cellular Apoptosis In The American Oyster (Crassostrea Virginica), Md Faizur Rahman

Theses and Dissertations

Global temperature increases due to anthropogenic activities. The effects of rising temperature are well documented in aquatic organisms. The American oyster is an ideal shellfish species to study on global warming and oxidative DNA damage. In this study, We observed the effects of high temperature on heat shock protein-70 (HSP70), 8-hydroxy-2’-deoxyguanosine (8-OHdG), ssDNA, dsDNA, caspase-3 (CASP3), BAX and γH2AX, a molecular marker of DNA damage, protein expressions in gills of oysters. Immunohistochemical results showed that elevated temperatures (28 and 32°C) significantly increased HSP70, 8-OHdG, dsDNA, γH2AX, BAX and CASP3 protein expressions in gills of oysters compare to control (24°C). In …


Effects Of Seasonal Variations And Heat Exposure On Antioxidant Expression And Redox Status In The American Oyster, Crassostrea Virginica: Field And Laboratory Studies, Md Sadequr Rahman Aug 2020

Effects Of Seasonal Variations And Heat Exposure On Antioxidant Expression And Redox Status In The American Oyster, Crassostrea Virginica: Field And Laboratory Studies, Md Sadequr Rahman

Theses and Dissertations

Increasing atmospheric temperatures influence physiological functions in aquatic organisms. In this study, I observed the effects of seasonal (field study) and laboratory heat exposure (16, 22, 26, and 30°C for 1-week) on prooxidant-antioxidant homeostasis in gills and digestive glands of American oyster. Histological analysis showed an increase in mucus secretion with elevated temperatures in both organs. Immunohistochemical analysis showed a significant increase of heat shock protein-70, dinitrophenyl, and 3-nitrotyrosine protein expressions with higher seasonal and laboratory temperatures indicating heat-induced oxidative and nitrosative stress. This was concomitant with gradual rise in cellular apoptosis. Interestingly, catalase and superoxide dismutase expressions also increased …


Development And Assessment Of An Environmental Dna (Edna) Assay For The Rio Grande Siren And Review Of Edna Metabarcoding Applications, Krista M. Ruppert Aug 2020

Development And Assessment Of An Environmental Dna (Edna) Assay For The Rio Grande Siren And Review Of Edna Metabarcoding Applications, Krista M. Ruppert

Theses and Dissertations

Environmental DNA (eDNA) assays have become a major aspect of amphibian surveys in the past decade. These methods are highly sensitive, making them well-suited for monitoring rare and cryptic species. Current efforts to study the Rio Grande Siren in south Texas have been hampered due to the cryptic nature of these aquatic salamanders. Arid conditions further add to the difficulty in studying this species, as many water bodies they inhabit are ephemeral, sometimes constraining sampling efforts to a short window after heavy rain. Additionally, sirens are known to cease activity and reside underground when ponds begin to dry or as …


Analysis Of The Facilitative Interaction Between Batis Maritima And Avicennia Germinans As A Mangrove Restoration Strategy, Javier R. Navarro Jul 2020

Analysis Of The Facilitative Interaction Between Batis Maritima And Avicennia Germinans As A Mangrove Restoration Strategy, Javier R. Navarro

Theses and Dissertations

The multiple experiments conducted in an Avicennia germinans forest in Laguna Vista, TX was focused on determining if facilitation theory could explain the behavior observed between the herbaceous halophyte Batis maritima and A. germinans. I hypnotized that a positive interaction exists but through which mechanisms of facilitation I did not know. The overarching theme of the study is the presence or absence of B. maritima. Facilitation theory tells us that positive interactions are most often found under stressful conditions such as those on the coastal tidal flat of the Laguna Madre. Locally B. maritima has been found to …


Detection And Enumeration Of Bacterial Pathogens In The American Oyster (Crassostrea Virginica), Mohammad Maruf Billah May 2020

Detection And Enumeration Of Bacterial Pathogens In The American Oyster (Crassostrea Virginica), Mohammad Maruf Billah

Theses and Dissertations

American oyster (Crassostrea virginica) is a popular seafood for its delicacy and high nutritional value. Based on increasing concern about contamination of bacterial pathogens in raw oyster, my research objectives have been focused on detection and enumeration of two important bacterial pathogens, Escherichia coli and Salmonella spp. in the American oyster in south Texas waters, local markets and controlled laboratory studies. Immunohistochemical and RT-PCR analyses showed substantial bacterial pathogen’s presence in gills and digestive glands of oysters collected from San Martin Lake and South Padre Island as well as local markets. Laboratory studies showed increasing trend of both …


Effects Of Artificial Substrates On The Recruitment Of Juvenile Fishes In The Northwestern Gulf Of Mexico, Stefany Salinas May 2020

Effects Of Artificial Substrates On The Recruitment Of Juvenile Fishes In The Northwestern Gulf Of Mexico, Stefany Salinas

Theses and Dissertations

Most artificial reefs (ARs) in the Gulf of Mexico are considered intermediate to high vertical relief structures which have recruited large predatory species indicating lack of juvenile habitat. Small, inexpensive ARs, standard monitoring units for the recruitment of reef fish (SMURFs), were deployed at eight sites at -20m as habitat for juvenile fishes to understand and determine the effects of substrate type on juvenile fishes recruitment. Each site contained four SMURFs with four treatments: small and large rubble (~10 and ~20cm), oyster shells, and bare. Surveys yielded 39 juvenile species, including one of the most economically important species, Lutjanus campechanus, …