Physical Sciences and Mathematics Commons^™

Chemical Synthesis Of Sensitive Dna, Komal Chillar

Dissertations, Master's Theses and Master's Reports

Over the past decades, researchers have tried various chemical methods to synthesize modified oligodeoxynucleotides (ODNs, i.e. short segments of DNAs). Traditional ODN synthesis methods require strong basic, and nucleophilic conditions for the deprotection and cleavage of the ODN from the solid support. However, the sensitive ODNs containing labile functionalities are vulnerable to such harsh conditions. Sensitive ODNs have a wide range of applications in research and pharmaceuticals. To synthesize sensitive ODNs, researchers devised different strategies but no practical methods have been developed. To overcome these challenges, we developed alkyl Dim alkyl Dmoc technology. This innovative technology uses weakly basic and …

Implementing Unmanned Aerial Vehicles To Collect Human Gait Data At Distance And Altitude For Identification And Re-Identification, Donn E. Bartram

Graduate Theses, Dissertations, and Problem Reports

Gait patterns are a class of biometric information pertaining to the way a person moves and poses. Gait information is unique to each person and can be used to identify and reidentify people. Historically, this task has been achieved through the use of multiple ground-based imaging sensors. However, as Unmanned Aerial Vehicles (UAVs) advance, they present the opportunity to evolve the process of persons identification and re-identification. Collecting human gait data using UAVs at distances ranging from 20m to 500m and altitudes ranging from 0m to 120m is a challenging task. The current biometric data collection methods, primarily designed for …

Using Virus Like Particle Conjugates Of Synthetic Targeting Compounds To Deliver Chemotherapeutic Drugs To Cancer Stem Cells, Austen W. Kerzee

Chemistry Theses

Cancer stem cells are a type of cell that have the properties of both cancer cells and stem cells. They can differentiate into other types of cancer cells, are resistant to conventional chemotherapeutics, and seem to contribute greatly to the metastasis and recurrence of cancer. Due to these properties, eliminating cancer stem cells would be greatly beneficial in the treatment of cancer. While there have been approved therapeutic methods for the removal of a few of the cancer stem cells types, treatment for most types of cancer stem cells are still in the experimental phase and have yet to be …

A Tale Of Two Working Landscapes, Sage C. Sutcliffe

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

A Road Map For Place Based Collaboration For Conflict Reduction, Joseph L. Zecher

Undergraduate Theses, Professional Papers, and Capstone Artifacts

No abstract provided.

Creation Of A Digital Storage System For Genome Sequencing Metadata, Jacquelin W. Olexa

Undergraduate Theses, Professional Papers, and Capstone Artifacts

As the field of computational genomics continues to expand in both potential and application, it is now more imperative than ever to ensure that massive genetic sequencing datasets are properly stored in an accessible manner. This project sought to establish a practical, user-friendly, secure system for a genomics research lab (the Good Lab; thegoodlab.org) at the University of Montana. A MySQL database and connected web application was ruled the best configuration to maximize utility and accessibility for the lab’s researchers. Building the logical framework for the database, creating the server, and sourcing data occurred over several months. The dataset ranged …

Building Detailed And Accurate Whole-Plant Concepts: A Morphometrics-Informed Reconstruction Of A Zosterophyll From The Lower Devonian Of Wyoming, Samar R. El-Abdallah

Cal Poly Humboldt theses and projects

The fragmentary state of plant fossils and the modular organization of plants make whole-plant reconstructions of fossil species desirable and feasible. Such reconstructions are key for integrating fossil species in systematic studies. The ca. 410 Ma Beartooth Butte Formation of Wyoming (USA) hosts the only rich Early Devonian plant assemblages in western North America, which fills a major gap in the phytogeography of this interval. I construct a whole-plant concept for a new zosterophyll from the Beartooth Butte Formation based on a detailed morphometric study. More than 600 fragments of the new zosterophyll were observed and 200 of those were …

Fire Severity Mediates Marten And Fisher Occurrence: Impacts Of The Dixie Fire On A Carnivore Community, Christopher James Collier

Cal Poly Humboldt theses and projects

The consumption of an astounding one million acres resulted from California’s largest single fire to date, the 2021 Dixie Fire. The social and economic losses associated with the fire were immediately apparent, but the effects on wildlife remained unknown. While previous research has suggested mixed or low severity fire may be beneficial to certain wildlife species, the responses to megafires are poorly understood for many carnivores. To better understand these responses to severe fire, I used a random sampling design stratified by burn severity to survey in and around the Dixie Fire footprint using baited camera stations. This allowed me …

Tropical Fish Study In Tahiti, French Polynesia, Miranda Brainard, Caitlyn Swango, Paityn Houglan, Richard Londraville

Williams Honors College, Honors Research Projects

In May of 2023, I embarked on an exciting research journey to Moorea, French Polynesia, alongside fellow students and faculty members from the University of Akron and Syracuse University. This expedition was part of the university-sponsored Tropical Vertebrate Biology course, where we delved into the exploration of various tropical species inhabiting the island, including sea urchins, geckos, and my primary focus, the blackspotted rockskipper.

My research team, composed of my co-authors and me, was particularly intrigued by the unique refuge-seeking behavior displayed by blackspotted rockskippers. These amphibious fish are renowned for their remarkable ability to inhabit tide pools and rocky …

Language Models For Rare Disease Information Extraction: Empirical Insights And Model Comparisons, Shashank Gupta

Theses and Dissertations--Computer Science

End-to-end relation extraction (E2ERE) is a crucial task in natural language processing (NLP) that involves identifying and classifying semantic relationships between entities in text. This thesis compares three paradigms for end-to-end relation extraction (E2ERE) in biomedicine, focusing on rare diseases with discontinuous and nested entities. We evaluate Named Entity Recognition (NER) to Relation Extraction (RE) pipelines, sequence-to-sequence models, and generative pre-trained transformer (GPT) models using the RareDis information extraction dataset. Our findings indicate that pipeline models are the most effective, followed closely by sequence-to-sequence models. GPT models, despite having eight times as many parameters, perform worse than sequence-to-sequence models and …

Factors Associated With Acoustic Bat Presence During Spring Emergence In The Appalachian Mountains Of Western Virginia, Emily Kirk Pody

Theses, Dissertations and Capstones

Studies of threats that bats face during hibernation have increased in response to white-nose syndrome (WNS), a fungal disease that has ravaged North American bat populations. However, impacts of WNS on bat ecology during spring emergence, when bats are potentially recovering from infection and allocating resources for reproduction, is relatively understudied. As more bat species become listed at the federal and state level, the need to understand the factors associated with spring emergence is critical for improving conservation guidelines and habitat management practices. Acoustic monitoring is an efficient method for monitoring bat presence for prolonged periods of time, giving biologists …

Mathematical Modeling And Analysis Of Inflammation And Tissue Repair: Lung Inflammation And Wound Healing In Corals Under Stress, Quintessa Hay

Theses and Dissertations

A variety of insults, including tissue injury and/or exposure to pathogen, elicit an immune response in many organisms. An improperly regulated immune response can result in deleterious effects to the organism. Here we present models for lung injury in young and old mice and models for wound healing in coral reefs.

It is well known that the immune response becomes less effective in older individuals. This is of particular interest in pulmonary insults such as ventilator induced lung injury (VILI) or lung infection. We extended a mathematical model for the inflammatory response to VILI and used experimental data to select …

When Brain Meets Artificial Intelligence, Lu Zhang

Computer Science and Engineering Dissertations

When we review the history of development of artificial intelligence (AI), we will find that brain science plays a pivotal role in fostering breakthroughs in AI, such as artificial neural networks (ANNs). Today, AI has made remarkable strides, particularly with the emergence of large language models (LLMs), surpassing expectations and achieving human-level performance in certain tasks. Nonetheless, an insurmountable gap remains between AI and human intelligence. It is urgent to establish a bridge between brain science and AI, promoting their mutual enhancement and collaborations. This involve establishing connections from brain science to AI (brain-inspired AI), and reversely, from AI to …

Factors That Influence Small Mammal Long Bone Morphology: An Analysis Of The Femora, Tibiae, And Humeri Of The Eastern Gray Squirrel (Sciurus Carolinensis), Tyler Everette Blake

Theses, Dissertations and Capstones

The goal of this study is to examine the effect of urbanization and latitude on bone morphology, specifically limb length and bone density among gray squirrels endemic to the eastern United States. This study’s hypotheses are as follows: gray squirrels occupying lower latitudes will have larger body sizes and longer limbs relative to body size than those at higher latitudes following Bergmann’s and Allen’s rules. Further, squirrels in urban habitats will have greater bone density than those in rural habitats. Results show moderate correlation between body mass and respective proxies and latitude following Bergmann’s rule. Weak correlations were found between …

The Comparison Of Different Wetland Fish Assemblages Over Time, Robert Edward Adelstein

Theses, Dissertations and Capstones

Wetlands provide essential ecosystem services. Historically, we have drained and filled 73% of wetlands for agricultural use throughout the United States from the 1780s to the 1980s (Dahl, 1990). A nationwide focus on restoring wetlands has since occurred. Literature on restored/mitigated wetlands is rife with examples that do and do not support the same ecosystem services as natural wetlands (Langston, 1997; Meil, 2014). Restoration of wetlands occurred at the Green Bottom Wildlife Management Area (GBWMA) over several decades. Various sections of the wetland were classified by age, water depth, and vegetation. One hypothesis was that differences in fish assemblage would …

Portable X-Ray Fluorescence Spectrometry For Sensing Salinity And Sodicity In Glacial Northern Great Plains Soils With Machine Learning Models, Adam Devlin

Electronic Theses and Dissertations

Saline and sodic soils are an increasing concern across the Northern Great Plains (NGP) due to factors of climate change and land management that are drawing geologically derived salts to the land surface. Traditional laboratory assessments, such as electrical conductivity (EC) and sodium adsorption ratio (SAR), are time and resource consumptive. Portable X-ray fluorescence (PXRF) may be a viable proximal sensing alternative, as it is able to provide elemental data in minutes, in situ or ex situ, and can directly quantify salinity-associated elements like Ca, Mg, and S. PXRF paired with predictive models has proven useful for a range of …

Effects Of Low-Cost, Low-Tech Tools For Riparian Restoration On Prairie Streams In Western South Dakota, James Andrew Joseph Bolyard

Electronic Theses and Dissertations

Despite making up less than two percent of the overall landscape in the arid and semi-arid western US, riparian areas are a crucial resource for agriculture, livestock, and wildlife. However, many have impaired function and reduced riparian cover. Low-Cost, Low-Tech Tools (LCLTT) are a subset of Process-Based Restoration (PBR) used for riparian restoration that were chosen for their cost-effectiveness and minimal technical requirements. LCLTT has been tested in mountainous areas of the western US but only recently implemented in the Northern Great Plains (NGP). Given their novelty as an approach toward restoration for the region, professional restoration and landowner communities …

The Hutton Project: Long-Term Agricultural Impacts On Soil Loss And Carbon Dynamics In Eastern South Dakota, Eli Halverson

Electronic Theses and Dissertations

Long-term and intensified agricultural land management has resulted in increased rates of soil erosion and has altered much of the carbon cycle at regional and global scales. Anthropogenic degradation of soil resources is a barrier to sustainable production, soil functioning, and ecosystem services. It is difficult to quantify the scope of pedogenic changes due to the lack of legacy data and short temporal scales. This study utilized decades to century-old soil information to quantify historical soil erosion losses and changes in soil carbon pools of eastern South Dakota soils. The results show that soils in the region have been significantly …

Species Distribution Modeling Of Aquilegia Brevistyla (Ranunculaceae): A Critically Imperiled Black Hills Disjunct Species, Eric Daniel Puetz

Electronic Theses and Dissertations

Unchecked human activity is contributing to rising levels of greenhouse gas emissions, changes in land use, altered disturbance/fire regimes, spread of invasive species, and loss of biological diversity and related breakdown of ecosystem services. Additionally, climatic shifts may lead to phenological mismatches between species and their environments if these changes outpace a species’ ability to adapt or migrate to a more suitable habitat. Isolated mountain populations are particularly threatened by unpredictable climatic conditions, as they may have limited migration corridors and often lower levels of genetic diversity to move or adapt, respectively. As these negative feedbacks compound on the landscape, …

Policy Recommendations For Tire Additive 6ppd And Its Derivative 6ppd-Q, Ashley E. King

Dissertations, Theses, and Masters Projects

Around 3.1 billion tires are produced around the world annually1. The antioxidant additive, 6PPD (i.e., N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine) is widely employed in passenger and commercial vehicle tires at 0.4-2% by mass to impede tire degradation2. Antioxidants are intended to migrate to tire surfaces and form protective films to prevent rubber oxidation. 6PPD is designed to react with oxidant species like ozone, intentionally forming chemical transformation products that can then escape from the tire and into the environment. 6PPD-Q (i.e., 2-anilino-5-[(4-methylpentan-2-yl)amino]cyclohexa-2,5-diene-1,4-dione) is one such transformation product.

After release and disbursement in the environment, 6PPD-Q is bioavailable to aquatic animals and mammals and acute …

C. Compactum Acts As A Comprehensive Climate Archive And Ecological Foundation In The Labrador Sea, Sadie Heckman

CMC Senior Theses

Clathromorphum compactum, a species of crustose coralline algae (CCA), is incredibly valuable for the future of high latitude ocean health, both as a comprehensive archive of changing ocean conditions, and ecologically as a foundational species for promoting biodiversity. Previous work establishes C. compactum as an effective climate proxy, and its life history provides several advantages for this use. C. compactum grow in nongeniculate, generally radial formations on hard substrates, over a wide distribution in mid-to-high latitude oceans and at subtidal depth ranges. Indeterminate growth leads to extreme longevity in C. compactum (Halfar et al., 2008), and growth rates are relatively …

Exploring The Consistency Of Flow Regimes Within And Among Ecoregions Of The Southeastern United States, Frank Paul Braun Iv

Electronic Theses and Dissertations

Human manipulation of river systems has long been a known contributor to the loss of freshwater biodiversity. By accounting for environmental causes of hydrologic variation among rivers, we can better understand how ecoregion mediates flow regimes and forecast species that may be at risk. Presumably, natural variation associated with ecoregion boundaries exerts strong influence on flow regimes, and may mediate relationships between other features (e.g., land use, dam operations) and hydrology. However, such between-ecoregion variation is poorly investigated, particularly at fine spatial and temporal scales. I characterized 10 hydrologic metrics, representing the five key dimensions of the flow regime (magnitude, …

Model-Based Deep Autoencoders For Clustering Single-Cell Rna Sequencing Data With Side Information, Xiang Lin

Dissertations

Clustering analysis has been conducted extensively in single-cell RNA sequencing (scRNA-seq) studies. scRNA-seq can profile tens of thousands of genes' activities within a single cell. Thousands or tens of thousands of cells can be captured simultaneously in a typical scRNA-seq experiment. Biologists would like to cluster these cells for exploring and elucidating cell types or subtypes. Numerous methods have been designed for clustering scRNA-seq data. Yet, single-cell technologies develop so fast in the past few years that those existing methods do not catch up with these rapid changes and fail to fully fulfil their potential. For instance, besides profiling transcription …

Covalent Inhibition Of Enzyme Sortase A As A New Pathway Against Bacterial Resistance Of Staphylococcus Aureus, Umyeena Bashir

Master's Theses

The continued rise of antibiotic-resistant bacteria, such as MRSA (methicillin-resistant Staphylococcus aureus), has made the discovery of novel antibiotics critical. While bacteriostatic and bactericidal antibiotics inevitably exert evolutionary pressure on bacteria to develop resistance, small molecules that target mechanisms of bacterial virulence present a promising alternative for treatment. Sortase A (Srt A), a cysteine protease of S. aureus, promotes bacterial virulence by covalently attaching proteins such as pilin to the bacterial surface, enabling bacterial adhesion to mammalian cells. Inhibitory studies of this enzyme have gained prominent interest as a new pathway for drug development since blocking this enzyme's ability to …

Adaptation Reshapes The Distribution Of Fitness Effects, Diego Tenoch Morales Lopez

Electronic Thesis and Dissertation Repository

The process of adaptation has been of interest since the XIX century, when Darwin first proposed the idea of natural selection. Since then, there has been a myriad of theoretical and empirical works that have expanded the field. From the many evolutionary insights these works have produced, a foundational idea is that spontaneous mutations in the genome of organisms can produce changes to their reproductive success that might confer an advantage for the mutant organisms with respect to their peers. Therefore, mutations drive adaptive evolution by virtue of their heritable effects on fitness. Empirical measures of the distribution of these …

Interactive Effects Of Sublethal Concentrations Of Fracking Biocides And Abandoned Mine Drainage On Amphipod Behavior, Kelly Lenhart

Electronic Theses and Dissertations

This thesis examined the sublethal effects of three pollutants, namely glutaraldehyde, 4,4-dimethyloxazolidine (DMO), and abandoned mine drainage (AMD), on amphipods. The primary objective was to investigate their combined effects on amphipods. The three pollutants, despite having the potential to combine in the environment, have not been studied to determine their potential for detrimental interactive effects which could result in unexpected environmental damage.

The research employed a series of experimental setups involving controlled exposure of amphipods to varying, putatively sublethal, concentrations of the chemicals of interest. Subsequently, effects were assessed via both behavioral and feeding assessments. To facilitate this analysis, novel …

Targeting Macrophytes: Increased Water Quality Through Optimized Vegetation Considerations For Constructed Wetlands, Austin Mcbrady

Masters Theses

This study of constructed wetland design investigated relationships between macrophyte species selection and planting density for water quality improvement. A lab-scale wetland was compared against a pilot-scale wetland in San Antonio, Texas at Mitchell Lake to measure differences in effluent water quality improvement using three native macrophyte species. Using a novel, two-phase method, a targeting macrophyte was identified from among other species based on its marked capability for improving water quality factors, then was planted in varied majority densities to compare differences in treatment effectiveness. The results of this study showed that this complimentary approach to wetland design displayed significant …

The Unheard Voices Of The Arkansas Delta: Living Through School Desegregation, Tameka D. Williams

ATU Theses and Dissertations 2021 - Present

This study was guided by the central research question: What are the lived experiences and perceptions of former African American teachers and students that endured segregation, desegregation, and integration in Arkansas' Delta? The phenomenological study used eight former African American teachers and student participants. Of the participants, there were four former teachers and four former students. In this study, the participants' voices are captured as they share their experiences and perception of each phase of education that influenced their lives and left an everlasting imprint. Through counternarratives, the participants provided insight into education in the Arkansas Delta during school segregation, …

Addressing Water Hyacinth (Pontederia Crassipes) Impacts On Aquatic Biota In Lake Okeechobee, Joseph Salerno

All HCAS Student Capstones, Theses, and Dissertations

The incursion of water hyacinth, Pontederia crassipes in Lake Okeechobee has resulted in management systems to be implemented to reduce the coverage of the invasive macrophyte. Its residence in the Lake Okeechobee ecosystem and the effects it has on organisms in the lake, whether it be positive or harmful is unknown. This study attempted to assess the potential effects that water hyacinth has on aquatic biota in Lake Okeechobee. Biotic data were collected on open water, water hyacinth covered, and native vegetation covered habitats via hook-and-line fishing, electrofishing, baited minnow traps, and the sampling of plant roots over a thirteen-month …

Biophysical Factors Affecting Habitat Suitability For Crassostrea Virginica, Jason D. Tilley

Dissertations

Oyster reefs provide a variety of important ecosystem services. However, the mortality rate of eastern oyster, Crassostrea virginica, the dominant species that produces oyster reefs in the northern Gulf of Mexico, is increasing at an alarming rate due to a variety of abiotic and biological factors. I examined how biophysical factors, including the less-studied fatty acid profiles of the suspended particulate matter on which oysters feed, influenced morphometric condition of C. virginica.

I sampled suspended particulate matter (SPM) and oysters in-situ in the western Mississippi Sound, which historically supported the majority of oyster production in Mississippi waters. Sampling …