Veterinary Preventive Medicine, Epidemiology, and Public Health Commons^™

Fusion Of Visible And Thermal Images Improves Automated Detection And Classification Of Animals For Drone Surveys, B. Santhana Krishnan, Landon R. Jones, Jared A. Elmore, Sathishkumar Samiappan, Kristine O. Evans, Morgan Pfeiffer, Bradley Blackwell, Raymond Iglay

United States Department of Agriculture Wildlife Services: Staff Publications

Visible and thermal images acquired from drones (unoccupied aircraft systems) have substantially improved animal monitoring. Combining complementary information from both image types provides a powerful approach for automating detection and classification of multiple animal species to augment drone surveys. We compared eight image fusion methods using thermal and visible drone images combined with two supervised deep learning models, to evaluate the detection and classification of white-tailed deer (Odocoileus virginianus), domestic cow (Bos taurus), and domestic horse (Equus caballus). We classified visible and thermal images separately and compared them with the results of image fusion. …

Biodiversity Indices And Random Forests Reveal The Potential For Striped Skunk (Mephitis Mephitis) Fecal Microbial Communities To Function As A Biomarker For Oral Rabies Vaccination, Matthew W. Hopken, Darby Gilfillan, Amy Gilbert, Antoinette J. Piaggio, Mikaela Samsel Hilton, James Pierce, Bruce Kimball, Zaid Abdo

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife disease surveillance and monitoring poses unique challenges when assessing rates of population vaccination, immunity, or infection prevalence. Non-invasively detected biomarkers can help reduce risk to both animal and field personnel during wildlife disease management activities. In this study, we investigated the utility of fecal microbiome data collected from captive striped skunks (Mephitis mephitis) in predicting rabies virus vaccination and infection status. We sequenced the hypervariable region 4 (V4) of the bacterial 16S gene and estimated alpha and beta diversity across timepoints in three groups of skunks: vaccination then rabies virus infection, sham vaccination then rabies virus infection, and rabies …

Determination Of Residue Levels Of Rodenticide In Rodent Livers Offered Novel Diphacinone Baits By Liquid Chromatography-Tandem Mass Spectrometry, David A. Goldade, Shane Siers, Steven C. Hess, Robert T. Sugihara, Craig A. Riekena

United States Department of Agriculture Wildlife Services: Staff Publications

A specific and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of the anticoagulant rodenticide diphacinone (DPN) in mouse and rat liver. Tissue samples were extracted with a mixture of water and acetonitrile containing ammonium hydroxide. The extracted sample was cleaned up with a combination of liquid-liquid partitioning and dispersive solid phase extraction. Chromatographic separation was achieved using a Waters X-Bridge BEH C-18 LC column (50 mm, 2.1 mm ID, 2.5 μm particle size) with detection on a triple quadrupole mass spectrometer in multiple reaction monitoring (MRM) mode. The monitored transition for DPN was m/z …

Controllable Factors Affecting Accuracy And Precision Of Human Identification Of Animals From Drone Imagery, Landon R. Jones, Jared A. Elmore, B. Santhana Krishnan, Sathishkumar Samiappan, Kristine O. Evans, Morgan B. Pfeiffer, Brad Blackwell, Raymond Iglay

United States Department of Agriculture Wildlife Services: Staff Publications

Surveying animal populations using drones (unoccupied aircraft systems [UAS]) provides numerous advantages; however, few best practices exist to survey animal communities with drones. Among myriad factors that can affect human identification and counts of animals from drone images, we focused on three factors typically controlled in the study design or by the drone pilot: flight altitude, camera angle, and time of day. Identifying interactions and patterns among these three variables represents an important first step to determining best survey practices. We used a drone to survey known numbers of eight animal decoy species, representing a range of body sizes and …

African Swine Fever, Vienna R. Brown, Julianna B. Lenoch, Courtney F. Bowden

United States Department of Agriculture Wildlife Services: Staff Publications

At the time of writing this chapter, a global pandemic of African swine fever (ASF) is ongoing with the virus having moved from Eastern Europe, Asia, and into the Caribbean—leaving swine production in devastation along the way. Due to the global spread of African swine fever virus (ASFV), the persistence of the virus, and the increasing number of endemic countries, this disease poses an imminent threat of introduction into North America and other countries that are currently ASF free. Throughout the chapter, we reference Eurasian wild boar (Sus scrofa) which are charismatic megafauna that are native to Europe and Asia. …

Role Of Social Structure In Establishment Of An Invasive Large Mammal After Translocation, Lindsay M. Clontz, Anni Yang, Sarah M. Chinn, Kim M. Pepin, Kurt C. Vercauterten, George Wittemyer, Ryan S. Miller, James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

Background

Data on the movement behavior of translocated wild pigs is needed to develop appropriate response strategies for containing and eliminating new source populations following translocation events. We conducted experimental trials to compare the home range establishment and space-use metrics, including the number of days and distance traveled before becoming range residents, for wild pigs translocated with their social group and individually.

Results

We found wild pigs translocated with their social group made less extensive movements away from the release location and established a stable home range ~5 days faster than those translocated individually. We also examined how habitat quality …

Outbreak Of Epizootic Hemorrhagic Disease In Captive Reindeer (Rangifer Tarandus), Emma H. Torii, Arno Wünschmann, Mia Kim Torchetti, Leo Koster, Albert Van Geelen, Randy Atchison, Anne Rivas

United States Department of Agriculture Wildlife Services: Staff Publications

In September 2020, an outbreak of epizootic hemorrhagic disease occurred in captive reindeer (Rangifer tarandus) and was associated with neurological signs and mortality. Four reindeer died or were euthanized after acute illness over a 12-day period. Affected reindeer displayed abnormal behavior, neurologic signs, lethargy, and/or lameness. The most consistent gross finding was dark red streaks throughout the adrenal gland cortices (4/4). One animal had acute hemorrhage involving the subcutis and skeletal muscles over the ventrolateral body wall and back, and abomasal serosa. Histologically, the most common lesions were adrenal gland cortical hemorrhage (4/4) with necrosis (3/4) and lymphoplasmacytic meningoencephalitis with …

Investigation Of Risk Factors For Introduction Of Highly Pathogenic Avian Influenza H5n1 Virus Onto Table Egg Farms In The United States, 2022: A Case–Control Study, Alice L. Green, Matthew Branan, Victoria L. Fields, Kelly Patyk, Stephanie K. Kolar, Andrea Beam, Katherine Marshall, Rachel Mcguigan, Matthew Vuolo, Alexis Freifeld, Mia Kim Torchetti, Kristina Lantz, Amy H. Delgado

United States Department of Agriculture Wildlife Services: Staff Publications

Introduction: The 2022–2023 highly pathogenic avian influenza (HPAI) H5N1 outbreak in the United States (U.S.) is the most geographically extensive and costly animal health event in U.S. history. In 2022 alone, over 57 million commercial and backyard poultry in 47 U.S. states were affected. Over 75% of affected poultry were part of the commercial table egg production sector. Methods: We conducted a case–control study to identify potential risk factors for introduction of HPAI virus onto commercial table egg operations. Univariate and multivariable analyses were conducted to compare farm characteristics, management, and biosecurity factors on case and control farms. Results: Factors …

Investigation Of Risk Factors For Introduction Of Highly Pathogenic Avian Influenza H5n1 Infection Among Commercial Turkey Operations In The United States, 2022: A Case-Control Study, Kelly A. Patyk, Victoria L. Fields, Andrea L. Beam, Matthew A. Branan, Rachel E. Mcguigan, Alice Green, Mia K. Torchetti, Kristina Lantz, Alexis Freifeld, Katherine Marshall, Amy H. Delgado

United States Department of Agriculture Wildlife Services: Staff Publications

Introduction: The 2022–2023 highly pathogenic avian influenza (HPAI) H5N1 outbreak in the United States (U.S.) is the largest and most costly animal health event in U.S. history. Approximately 70% of commercial farms affected during this outbreak have been turkey farms. Methods: We conducted a case-control study to identify potential risk factors for introduction of HPAI virus onto commercial meat turkey operations. Data were collected from 66 case farms and 59 control farms in 12 states. Univariate and multivariable analyses were conducted to compare management and biosecurity factors on case and control farms. Results: Factors associated with increased risk of infection …

Impact Of Pistachio Shell Biochar In Finishing Beef Cattle Diets, Levi J. Mcphillips, Mitchell M. Norman, J. L. Sperber, Andrea K. Watson, Galen E. Erickson

Nebraska Beef Cattle Reports

A 190-day finishing experiment was conducted to evaluate effects of feeding biochar on methane and carbon dioxide production, animal performance and carcass traits in beef steers. A high concentrate feedlot diet was used, and two dietary treatments were compared, 0 or 1% biochar as % of diet dry matter. Cattle were monitored using a calorimetry emissions barn to quantify production of methane and carbon dioxide. There were no differences in emissions, performance, or carcass characteristics for cattle fed the control diet or with biochar supplemented into the diet.

2023 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

Cow-Calf Management

• Comparison of Semi-confined and Pasture-based August Calving Beef Cow Systems
• Including Gene Edited Sires in Genetic Evaluations
• Impact of Planning Horizon Length on Breeding Objectives and Resulting Selection Decisions
• Effect of Increasing Energy 30 days Prior to Artificial Insemination in Beef Heifers
• Extending Melengestrol Acetate Treatment from Fourteen to Eighteen Days in Beef Heifers
• Artificial Insemination of Beef Heifers with Multi-Sire Sexed Semen

Growing Calf and Yearling Management

• Timing of Implant Use in the Backgrounding System
• Effects of Strategic Supplementation on Return to Management and Performance of Yearling Cattle

Forage Evaluation

• Forage Evaluation of Crested Wheatgrass
• Evaluation of …

Analysis Of Spoilage Bacteria Present In Vacuum Packaged Chilled Beef Treated With Organic Acids, Samuel C. Watson, Rebecca A. Furbeck, Byron D. Chaves, Samodha C. Fernando, Gary Sullivan

Nebraska Beef Cattle Reports

Preventing the spoilage of fresh, chilled beef is crucial for maintaining market value. Since organic acids are regularly used in the beef industry for pathogen control, their ability to improve the shelf life of fresh, chilled beef was evaluated. Fresh, chilled beef was individually treated with 4.5% lactic acid, 2.5% Beefxide^®, or 380 ppm peroxyacetic acid. After storage, Lactococcus, a genus of lactic acid bacteria, became the most common spoilage organism across all treatment and control samples. Organic acid treatments were not able to slow the growth of this genus and may not be an effective method to …

Greenhouse Gas Emissions From Two Beef Systems From Birth To Slaughter In Eastern Nebraska, Levi J. Mcphillips, Zac E. Carlson, Andrew E. Suyker, J. C. Macdonald, Tala Awada, Jane Okalebo, Shree Dangal, Rick R. Stowell, Andrea K. Watson, Galen E. Erickson

Nebraska Beef Cattle Reports

Methane emissions from growing and finishing calves born into a spring calving, conventional cow system or a summer calving, partially-confined cow system were compared. Cows fed a restricted diet of corn-byproduct and grain residues in confinement produced less methane and carbon dioxide per day compared to cows grazing pasture or cover crop. Calves weaned from the confinement-based production system were smaller at weaning and compensated with greater gain during the growing phase. More days on feed in the finishing phase were needed for the calves from the confinement system to reach same backfat thickness. Over the entire growing and finishing …

Ponderosa Pine Wood Biochar Used As An Emissions Reduction Strategy In A Finishing Beef Cattle Diet, Holly A. Heil, Braden C. Troyer, Mitchell M. Norman, Andrea K. Watson, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing feedlot experiment was conducted to evaluate the impact of feeding biochar on methane and carbon dioxide production, performance, and carcass characteristics in beef cattle. Two dietary treatments were evaluated; 0 or 1% biochar in a high concentrate diet comprised of dry-rolled corn, high moisture corn, Sweet Bran, and corn silage. Ponderosa pine wood biochar was added into the diet at 1% dry-matter displacing a 1% dry-matter blend of corn. Cattle were monitored using a calorimetry emissions barn to capture methane and carbon dioxide production. Emissions production, performance and carcass characteristics did not differ between cattle fed a control …

Effect Of Alga Bio 1.0 On Reducing Enteric Methane Emission From Cattle, Reba L. Conlin, Kassidy K. Buse, Andrea K. Watson, Galen E. Erickson, Paul J. Kononoff

Nebraska Beef Cattle Reports

An experiment was conducted to determine the effect of Alga Bio 1.0 inclusion on methane and carbon dioxide emissions along with diet digestibility. Three treatments were evaluated with 0, 69, and 103 grams per day Alga Bio 1.0 fed as a top dress in a corn-based diet. Indirect calorimetry headboxes were utilized to evaluate gas production with 12 cows in 4 replicated 3x3 Latin squares. There was a 39% reduction in methane per lb of dry matter intake for cattle fed 69 g of Alga Bio 1.0 and 63% reduction when cattle were fed 103 g of Alga Bio 1.0 …

Quantifying Residual Feed In A Fence-Line Feedlot Bunk Using Depth Camera Imaging Techniques, Jean Niwenshuti, Galen E. Erickson, Tami M. Brown-Bandl, Eric T. Psota, Yijie Xiong

Nebraska Beef Cattle Reports

Feed bunk management requires intensive labor and relies on manual observation to estimate the amount of residual feed in the bunk. Alternative and innovative technologies were used to estimate the weight of residual feed in a concrete fence-line bunk using a depth camera. Depth cameras capture the distance between the camera and the object in their field of view. This study used a time-of-flight depth camera (Azure Kinect, Microsoft) to estimate the weight of residual feed in a partial fence-line concrete bunk using 11 common feed ingredients. The camera was fastened approximately 3.3 ft above the center of the bunk …

Effects Of Corn Processing And Silage Inclusion In Feedlot Diets On Steer Performance, Jessica L. Miller, Nolan R. Meier, K. H. Jenkins Pas, Galen E. Erickson, Pablo L. Loza

Nebraska Beef Cattle Reports

A feelot study compared the effects of corn silage inclusion on steer performance and carcass characteristics withing dry-rolled corn diets and steam-flaked corn diets. Treatments included four corn silage inclusions as 0, 15, 30, 45% of dry matter in both steam-flaked corn and dry-rolled corn base diets. Feeding a steam-flaked based corn diet increased average daily gain by 7.8% and improved feed conversion by 6.8% when compared to steers fed a dry-rolled corn diet. As corn silage inclusion increased, feed conversion increased linearly. When fed to the same days on feed carcass adjusted final body weight, hot carcass weight, and …

Effect Of Enogen Feed Corn Inclusion In Conventional And Natural Finishing Cattle Diets, Jessica Miller, K. H. Jenkins Pas, Galen E. Erickson, Pablo L. Loza

Nebraska Beef Cattle Reports

Increasing the extent of starch digestibility during finishing could allow producers to improve cattle efficiency. A finishing performance study was conducted to determine the effect of Enogen Feed Corn inclusion as dry-rolled corn and corn silage in comparison to a control corn hybrid within natural and conventional feeding programs for heifers and steers. Cattle in the conventional feeding program received implants and the ration included feed additives, while cattle on the natural program were not implanted and the ration did not contain feed additives. The inclusion of Enogen Feed Corn had no impact on steer or heifer finishing performance. The …

Effect Of Corn Processing On Steer Performance And Fecal Starch Content, Jessica L. Miller, Braden C. Troyer, Levi J. Mcphillips, Mitchell M. Norman, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Processing corn as high-moisture corn increases starch digestion and improves cattle efficiency when fed blended with dry rolled corn in finishing rations. A finishing study evaluated the effect of corn processing method (dry-rolled corn or 2:1) high-moisture corn to dry rolled corn blend) on performance of calf-fed steers. Corn processing method did not affect average daily gain; however, steers fed a high-moisture corn and dry-rolled corn blend consumed 1.1 lb/day less than steers fed a dry-rolled corn diet. Feeding high-moisture corn and dry-rolled corn blend diets improved feed efficiency by 5.2% compared to steers fed dry-rolled corn. Fecal starch content …

Supplemental Lysine In Finishing Cattle Diets, Hanna Cronk, Zac Carlson, Mitch Norman, Levi Mcphillips, Andrea Watson, Galen E. Erickson, Gary Ducharme

Nebraska Beef Cattle Reports

The objective of this experiment was to evaluate the effects of increasing lysine supply in finishing beef cattle diets. Crossbreed steers (n=120, BW=577 ± 2 lb) were individually fed using a Calen Gate system for 195 days. Animals received a common finishing diet (63% corn, 15% corn silage and 15% distillers grains) with 0, 1, 2, 3, 4, 5, 6, or 7 g per day of supplemental rumen bypass lysine. High levels of lysine (5 or more g per day) in the diet decreased dry matter intake, carcass adjusted body weight with no impact on carcass adjusted feed efficiency. Hot …

Impact Of Removing 20% Distillers Grain After One-Third Or Two-Thirds Of The Feeding Period On Performance Of Finishing Yearling, Sofia Suarez Lorences, Braden C. Troyer, Mitch M. Norman, Pablo L. Loza, Rick Stock, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing study evaluated the effect of removing modified distillers grains plus solubles after one-third or two-thirds of the feeding period on performance and carcass characteristics of yearling steers. Treatment diets included 20% modified distillers for the entire feeding period; 20% modified distillers for two-thirds of the feeding period and then 0%; and 20% modified distillers for one-third of the feeding period, then 0%. Removing modified distillers from the diet decreased average daily gain, final body weight and dry matter intake and tended to increase feed conversion. Cattle fed 20% modified distillers throughout the entire feeding period had greatest hot …

Impact Of Constant Inclusion Or Decreasing Inclusion Of Distillers Grains With High-Quality Or Low-Quality Roughage On Finishing Cattle Performance, Sofia Suarez Lorences, University Of Nebraska - Lincoln, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing trial was conducted to evaluate high-quality or low-quality roughage inclusion in diets containing three concentrations of modified distillers grains plus solubles on finishing cattle performance. In a randomized block design, steers were fed according to a 2x3 factorial arrangement with two roughage sources (6% corn stalks versus 12% corn silage) in three diets containing 0, 15, or decreasing inclusion of distillers (30, 15, then 0% across the feeding period). No interactions were observed between distillers inclusion and roughage source except for intake. Steers fed corn silage consumed less, gained the same, and had slightly better feed conversions Steers …

Evaluate The Effect Of Corn Processing, Drying Distillers Grains, Oil Removal From Distillers Grains, And Distillers Inclusion On Cattle Performance, Braden C. Troyer, Elliott James Dennis, Alfredo Dicastanzo, Galen E. Erickson

Nebraska Beef Cattle Reports

An analysis of over 9,300 head of cattle and 980 pen means was conducted to evaluate the effect of corn processing, drying distillers grains, oil removal from distillers, and distillers inclusion on cattle performance. This analysis looked at both steam-flaked corn and high-moisture corn or dry-rolled corn or a blend of the latter two grains and their effects on performance with and without distillers grains. Additionally, wet, modified, and dried distillers grains were analyzed as both full fat or de-oiled products at various dietary concentrations with each corn type as the primary cereal grain to determine performance responses. There was …

Effects Of Individual Sweet Bran Components In Beef Finishing Diets On Nutrient Digestion, Rebecca L. Sjostrand, Rittikeard Prachumchai, Maggie Youngers, Rick A. Stock, J. C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Sweet Bran is a branded wet corn gluten feed recognized for improving rumen health, energy intake, and gains in finishing cattle. Eight ruminally cannulated steers were utilized in a replicated 4 x 4 Latin Square design to evaluate the effect of individual Sweet Bran components on total tract digestibility and rumen fermentation parameters. Three Sweet Bran components (solvent extracted germ meal, corn bran, and mixed steep) were included at 40% of diet dry matter in their respective treatment, with a steam-flaked corn control diet. Total tract dry matter and organic matter digestibility were least for bran, intermediate for solvent extracted …

Evaluation Of Lactiproflx In An Acidosis Challenge Model, Samantha K. Wagner, Rebecca J. Sjostrand, Tyler J. Spore, Mark E. Corrigan, Galen E. Erickson, J. C. Macdonald

Nebraska Beef Cattle Reports

An acidosis challenge study was conducted comparing different administration techniques for LactiproFLX, a direct fed microbial product containing Megaspharea elsdenii (a lactate-utilizing bacteria) for the prevention of acidosis. Four treatments were utilized in a randomized block design with 24 ruminally cannulated steers. Treatments consisted of a control group which did not receive the product, a group which received the commercial dose of the product four days before the acidosis challenge, one which received the commercial dose of the product one day before the challenge, and one which received ten times the commercial dose one day before the challenge. No differences …

Evaluation Of Encapsulated Megasphaera Elsdenii In An Accelerated Beef Step-Up Program And An Acidosis Challenge Event, Cindy D. Mansfield, Rebecca L. Sjostrand, Braden C. Troyer, Mitchell M. Norman, Tyler Spore, Mark Corrigan, Galen E. Erickson, J. C. Macdonald

Nebraska Beef Cattle Reports

A 100-day metabolism study with 40 ruminally cannulated steers, individually fed, was conducted to determine the efects of daily feeding of encapsulated Megasphaera elsdenii along with a one-time dose of Lactipro NXT on dry matter intake, rumen organic acid concentration, lactate disappearance and native and specific strains of Megasphaera elsdenii concentration following an acidosis challenge. Steers fed Megasphaera elsdenii daily had greater intake after an acidosis event. Cattle fed daily Megasphaera elsdenii also had a faster rate of lactic acid disappearance after an acidosis event. Feeding Megasphaeara elsdenii daily may result in a faster recovery time, after an acidosis event, …

Effect Of Ad Libitum Vs. Limit Feeding Program At Receiving On Morbidity And Performance Of Feedlot Calves, Rebecca A. Funk, Braden C. Troyer, Levi J. Mcphillips, Mitchell M. Norman, Galen E. Erickson

Nebraska Beef Cattle Reports

A two-phase study was conducted to determine the effects of two different feed delivery strategies during the receiving period of feedlot calves. Calves were fed either by limit-feeding at approximately 75% of ad libitum, or ad libitum feed offerings for the 30-d receiving period to determine effects on health and performance. During the receiving period, average daily gain and total weight gained was increased for the ad libitum treatment. No differences between ad libitum and limit-fed treatment groups were observed in either feed to gain or morbidity rates. During the second phase of the trial, a subset of calves was …

Effect Of Species And Maturity On Small Grain Silage Yield And Quality, Abigail M. Sartin, Kallie J. Calus, Morgan T. Grabau, Alyssa K. Kuhn, Mary E. Drewnoski, D. D. Redfearn

Nebraska Beef Cattle Reports

A study was conducted to determine the effect of species and maturity on yield and nutritive value of winter-hardy small cereal grains used for silage. Three species were evaluated: cereal rye, winter triticale, and winter wheat at four different stages of maturity: boot, pollination, milk, and soft dough. As species matured, yield increased across all stages, but crude protein (CP) and digestible organic matter (DOM) decreased, except at soft dough where there was a slight increase in DOM. Crude protein was greatest at the boot stage at 17.7% and least at soft dough at 9.8%. When comparing species, rye and …

Evaluation Of Ankom F58 Filter Bags Compared To Beakers For Analysis Of Neutral Detergent Fiber, Sawyer R. Kappel, Rebecca L. Sjostrand, J. C. Macdonald

Nebraska Beef Cattle Reports

Feed ingredient, feed refusals, duodenal and fecal samples were analyzed to compare two methods of determining neutral detergent fiber. All samples were weighed into Ankom F58 fiber bags and Analyzed using an Ankom 2000 automated fiber Analyzer. Results were then compared to the Van Soest beaker method. The fiber values determined from both methods were within 3.5% of one another, with the beaker method being consistently greater compared to the Ankom method, except for fecal samples. Variability in fiber estimates for ingredients, feed refusals, and feces translated to substantial inconsistency in estimated neutral detergent fiber digestibilities among treatments. It is …

Forage Evaluation Of Crested Wheatgrass, Kelton C. Adair, K. H. Jenkins Pas, Andrea K. Watson, James C. Macdonald

Nebraska Beef Cattle Reports

An experiment evaluated the forage value of crested wheatgrass (CWG) harvested throughout the grazing season near Sidney, Nebraska over a two-year period (2019-2020). The purpose of the evaluation was to determine forage quality and rumen undegradable protein (RUP) content to help producers with supplementation decisions for cattle grazing monoculture CWG pastures. In vitro dry matter disappearance quadratically decreased from 54% in May to 37% in September of 2019, with no changes throughout 2020, averaging 43%. In both years, crude protein (CP) decreased throughout the growing season while rumen undegradable protein (RUP) increased (as % CP). Digestible RUP was less than …