Open Access. Powered by Scholars. Published by Universities.®

Veterinary Pathology and Pathobiology Commons™

Open Access. Powered by Scholars. Published by Universities.®

Louisiana State University

Articles 61 - 90 of 104

Full-Text Articles in Veterinary Pathology and Pathobiology

Comparative Roles Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoproteins In Cytoplasmic Virion Egress, Hyuncheol Lee Jan 2008

Comparative Roles Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoproteins In Cytoplasmic Virion Egress, Hyuncheol Lee

LSU Master's Theses

HSV-1 acquires its final envelope by budding into cytoplasmic vesicles thought to be derived from Trans-Golgi Network (TGN) membranes. This process is facilitated by interactions among the carboxyl termini of viral glycoproteins and tegument proteins. To investigate the relative importance of different viral glycoproteins in cytoplasmic virion morphogenesis, a set of recombinant viruses were constructed silencing expression of the glycoprotein E (ΔgE), the carboxyl terminus of glycoprotein D (gDΔcp), and the membrane protein UL20 (ΔUL20). In addition, recombinant viruses were constructed having the ΔgE+gDΔcp, and the ΔgE+ΔgM (glycoprotein M) deletions. These recombinant viruses were constructed using the double-red, site-directed mutagenesis …

Go to article

Molecular Determinants Of Kaposi's Sarcoma-Associated Herpesvirus Tumorigenicity, Haixia Kong Jan 2008

Molecular Determinants Of Kaposi's Sarcoma-Associated Herpesvirus Tumorigenicity, Haixia Kong

LSU Master's Theses

A conditional silencing system using anti-gB siRNAs was devised to investigate the structure and function of the KSHV gB. Transient co-transfection of plasmids constitutively expressing gB and anti-gB siRNAs in 293 cells substantially inhibited gB mRNA levels and protein production. Similarly, transient expression of siRNAs into the basal cavity-based lymphoma cells (BCBL-1) caused substantial reduction of gB transcription and protein synthesis. TaqMan real-time PCR and infectivity assays showed that gB was essential for virion egress and infectivity. Transfection of a codon-optimized gB not recognized by the anti-gB siRNAs, efficiently rescued virion egress and infectivity in BCBL-1 cells in the presence …

Go to article

Novel Oncolytic Herpesviruses For Breast Cancer Treatment, Anna Israyelyan Jan 2008

Novel Oncolytic Herpesviruses For Breast Cancer Treatment, Anna Israyelyan

LSU Doctoral Dissertations

Oncolytic herpes simplex virus type-1 (HSV-1) has shown great potential as an effective agent for cancer therapy ¨C oncolytic virotherapy. To enhance oncolytic capabilities of previously reported agents, new oncolytic and fusogenic HSV-1 OncSyn and OncdSyn viruses were constructed based on wild type HSV-1 (F) strain. To provide for safety and tumor selectivity, the viruses carried a large deletion including one of the two ¦Ã134.5 genes. The ¦Ã134.5 gene, a major neurovirulence factor, was replaced by a gene cassette constitutively expressing the red fluorescent protein. Homologous recombination was utilized to transfer the fusogenic gBsyn3 mutation to the viral genome to …

Go to article

Characterization Of A Virulence Related Hypothetical Protein In Edwardsiella Ictaluri, Ildiko Katalin Polyak Jan 2007

Characterization Of A Virulence Related Hypothetical Protein In Edwardsiella Ictaluri, Ildiko Katalin Polyak

LSU Master's Theses

Although the biochemical characterization of E. ictaluri, the subsequent disease progression of enteric septicemia of catfish (ESC), and the associated pathologic lesions are well characterized, the mechanism of invasion of E. ictaluri into a susceptible host is poorly understood. Identification and confirmation of virulence factors and associated genes of E. ictaluri is crucial to elucidating the pathogenesis of this important disease. A signature tagged mutagenesis (STM) study conducted by Thune et al. (2006) identified 50 E. ictaluri clones with transposon insertions in genes potentially involved in pathogenesis. A specific STM mutant, 233PR, carrying a transposon insertion in a gene encoding …

Go to article

Evaluation Of Selected Immune Response To Haemonchus Contortus In Gulf Coast Native Compared To Suffolk Lambs, Krishna P. Shakya Jan 2007

Evaluation Of Selected Immune Response To Haemonchus Contortus In Gulf Coast Native Compared To Suffolk Lambs, Krishna P. Shakya

LSU Doctoral Dissertations

Haemonchus contortus is one of the major nematode parasites causing substantial economic losses in small ruminant farming worldwide. Recently, effect of anthelmintic treatment has decreased due to an increasing problem of nematode populations that have developed resistance to anthelmintics. There are certain breeds of sheep that are identified as being relatively resistant to the parasite including Gulf Coast Native (Native) sheep. Understanding the mode of immune response that helps these breeds of sheep control infection could help design vaccines and enhance control programs. This experiment was designed to evaluate the immunological responses of Native, compared with susceptible Suffolk sheep that …

Go to article

Depletion Of 32-Kbp Circular Plasmids From Borrelia Burgdorferi, Amanda Paige Derouen Polito Jan 2007

Depletion Of 32-Kbp Circular Plasmids From Borrelia Burgdorferi, Amanda Paige Derouen Polito

LSU Master's Theses

The Lyme disease spirochete Borrelia burgdorferi has a very unusual genome composed of one linear chromosome and up to 21 linear and circular plasmids. Several plasmids are known to be important either for mammalian infection or tick colonization. A single spirochete harbors up to 7 different cp32 plasmids; however, nothing is known about their role in mammalian infection. The plasmids in this family are well maintained during in vitro cultivation, making it difficult to study their functions. To effectively deplete the plasmids, an 8kbp fragment containing essential elements for replication and partitioning in B. burgdorferi was amplified from one of …

Go to article

Influence Of Tumor Necrosis Factor-Alpha And Minocycline On Microglia And Macrophage Activation During Polytropic Retrovirus Infection, Meryll E. Corbin Jan 2007

Influence Of Tumor Necrosis Factor-Alpha And Minocycline On Microglia And Macrophage Activation During Polytropic Retrovirus Infection, Meryll E. Corbin

LSU Master's Theses

Microglia/macrophage activation has been associated with the pathogenesis of various neurological diseases including human immunodeficiency virus encephalitis, transmissible spongiform encephalitis, and Alzheimer's disease (AD). In vitro studies have indicated a role for TNFα in activating these cells which leads to their migration, proliferation, and secretion of proinflammatory cytokines and chemokines that may potentially damage brain tissue. In the current study, we analyzed the phenotype of microglia and macrophages enriched from wild type and TNFα deficient mice infected with a neurovirulent murine retrovirus. Although TNF receptors CD120a and CD120b were expressed on both microglia and macrophage population, unaltered by either retrovirus …

Go to article

The Role Of Toll-Like Receptor 7 In The Neuropathogenesis Of Retrovirus Infection In Neonates, Stephanie Diane Lewis Jan 2007

The Role Of Toll-Like Receptor 7 In The Neuropathogenesis Of Retrovirus Infection In Neonates, Stephanie Diane Lewis

LSU Master's Theses

Viral infections of the central nervous system (CNS) in infants are rare; however, they are associated with high morbidity and mortality rates. These virus infections often induce strong innate immune responses in the brain including: the production of cytokines and chemokines, the activation of astrocytes and microglia and the recruitment of macrophages. Innate immune responses are often initiated by toll-like receptors (TLR). Several studies have demonstrated that toll-like receptor 7 (TLR7) can be stimulated by single-stranded RNA from multiple viruses. In the current study, we examined the mechanism by which TLR7 contributes to neuroinflammation in the neonatal brain using a …

Go to article

Genetics Of Herpes Simplex Virus Type-1 Tegument Proteins Involved In Virion Morphogenesis And Egress, Preston A. Fulmer Jan 2007

Genetics Of Herpes Simplex Virus Type-1 Tegument Proteins Involved In Virion Morphogenesis And Egress, Preston A. Fulmer

LSU Doctoral Dissertations

Herpes simplex virus type-1 (HSV-1) morphogenesis occurs in multiple stages within infected cells. Initially, the virion capsid assembles within the nucleus and buds through the nuclear membrane into the cytoplasm. Within the cytoplasm, additional tegument proteins attach to the capsid and the fully tegumented capsids bud into trans-Golgi network (TGN) derived vesicles. Enveloped virions are ultimately secreted to extracellular spaces. The process by which the cytoplasmic capsids bud into TGN-derived vesicles is not well understood. The prevalent model calls for specific interactions among viral tegument proteins and membrane proteins and glycoproteins embedded within TGN membranes. To further investigate the roles …

Go to article

Brucella Melitensis: The Evaluation Of A Putative Hemagglutinin Gene's Effect On Virulence In The Caprine Model, Quinesha Laticia Perry Jan 2007

Brucella Melitensis: The Evaluation Of A Putative Hemagglutinin Gene's Effect On Virulence In The Caprine Model, Quinesha Laticia Perry

LSU Doctoral Dissertations

Brucella melitensis is a facultative intracellular bacterial pathogen that causes abortions in goats and sheep and Malta fever in humans. The zoonotic disease brucellosis causes severe economic losses in the Mediterranean region and parts of Africa, Asia, and Latin America. With the completion of the genomic sequences of B. abortus 2308 and B. melitensis 16M, no classical virulence factors were found; and the chromosomes were virtually identical. However, in B. melitensis, a putative hemagglutinin gene was identified which is absent in B. abortus. The possibility of the hemagglutinin gene being a potential virulence factor was evaluated via gene replacement/deletion in …

Go to article

The Epidemiology Of West Nile Virus In Louisiana, Karen Ruth Gruszynski Jan 2006

The Epidemiology Of West Nile Virus In Louisiana, Karen Ruth Gruszynski

LSU Doctoral Dissertations

West Nile virus (WNV), a member of the genus Flavivirus transmitted by mosquitoes, first appeared in the New York in 1999. Within five years WNV was detected throughout the contiguous 48 states causing disease in reservoirs and accidental hosts alike. In Louisiana, WNV was first detected in 2001 with one human case, ten equine cases, and six dead birds reported. The introduction of WNV into Louisiana presented an unique opportunity to observe an emerging disease unfold, so a study was launched to gain insight into the epidemiology of WNV in Louisiana. The first component, an environmental predictive model for West …

Go to article

Brugia Phangi: Effects Of Third Stage Larvae Es Immunization On Early Migration And Parasite Establishment In Mongolian Gerbils (Meriones Unguiculatus), Ginger Ann Robertson Jan 2006

Brugia Phangi: Effects Of Third Stage Larvae Es Immunization On Early Migration And Parasite Establishment In Mongolian Gerbils (Meriones Unguiculatus), Ginger Ann Robertson

LSU Master's Theses

Brugia infections occur via the bite of an infected mosquito. Third stage infective larvae (L3) deposited on the skin during feeding migrate into the bite wound, through skin and into the lymphatic system. It is hypothesized that L3 excretory/secretory products (ES) are important in this initial phase of the infection. A model for these early migrations has been established by inoculating L3s into the dermis (ID) of the permissive gerbil host. In this model, most L3s injected ID in the louer hind limb travel to the popliteal lymph node by 3 days post infection. Adult parasites are located primarily in …

Go to article

Effect Of Vaccination Of Goats With H-Gal-Gp And H11 Antigens From Intestinal Membrane Cells Of Haemonchus Contortus, Donya Dupree Olcott Jan 2006

Effect Of Vaccination Of Goats With H-Gal-Gp And H11 Antigens From Intestinal Membrane Cells Of Haemonchus Contortus, Donya Dupree Olcott

LSU Master's Theses

Extracts of adult Haemonchus contortus were purified and used as a vaccine against the blood feeding parasite in goats as previously studied in sheep. The proteins used were H11 and H-gal-GP, hidden gut antigens from the microvillar membrane of the gut of the worm and combined with Quil A as adjuvant, then administered to a group of goats kept on concrete then artificially infected with H. contortus. The control group received Quil A injections and also infected. This study was performed to analyze the effects of the H11/H-gal-GP vaccine when given to goats as compared to sheep. The trial showed …

Go to article

Power Analysis For A Mixed Effects Logistic Regression Model, Yinmei Li Jan 2006

Power Analysis For A Mixed Effects Logistic Regression Model, Yinmei Li

LSU Doctoral Dissertations

In herd health studies, the mixed effects logistic regression model with random herd effects are commonly used for modeling clustered binary data. These models are well developed and widely used in the literature, among which is the logistic-normal regression model. In contrast to the rich literature in modeling methods, the sample size/power analysis methods for such mixed effects models are sparse. The sample size/power analysis method for the logistic-normal regression model is not readily available. This study is to develop a power analysis/sample size estimation method for the logistic-normal regression model. Extended from the sample size method for the likelihood …

Go to article

Racial Disparities, Birth Outcomes, And Changing Demographics Of East Baton Rouge Parish, Louisiana, Misty D. Richard Jan 2006

Racial Disparities, Birth Outcomes, And Changing Demographics Of East Baton Rouge Parish, Louisiana, Misty D. Richard

LSU Doctoral Dissertations

Racial and socioeconomic disparities persist throughout the country regardless of which specific disparity is studied; however, some geographic regions experience more significant racial disparities. East Baton Rouge Parish, Louisiana has a large racial disparity among birth outcomes. Many factors impact the degree of racial disparities, some of which include racial segregation, isolation, or centralization, access to and quality of medical care, and factors of the neighborhood environment. EBRP has undergone dramatic shifts in the demographics of its residents. The purpose of this dissertation was to study the demographic changes in the population; as well as to study the disparities among …

Go to article

Voluntary Exercise In The C57b1/6j Mouse: Phenotypic Effects Of Varying Dietary Fat Levels And Hippocampal Gene Expression Differences Between High-Level And Low-Level Exercisers, Leslie Debardeleben Mclaughlin Jan 2005

Voluntary Exercise In The C57b1/6j Mouse: Phenotypic Effects Of Varying Dietary Fat Levels And Hippocampal Gene Expression Differences Between High-Level And Low-Level Exercisers, Leslie Debardeleben Mclaughlin

LSU Doctoral Dissertations

The drive to exercise voluntarily likely results from complex interactions between genes in many organ systems and various psychological parameters, such as motivation and the perception of fatigue. Reproducible variations in exercise intensity and duration are well established in laboratory rodents, but the genes responsible remain largely unknown. Also, to date, studies addressing the adaptive changes to exercise that might prevent dietary-induced obesity have focused primarily on energy intake and nutrient oxidation/partitioning, as opposed to genetics. We hypothesize that increased voluntary physical activity may be a normal mechanism in certain rodent strains to deter dietary-induced obesity and that in an …

Go to article

Neural Stem Cells As A Delivery Vector For Chemokine Expression In The Central Nervous System, Mark Winston Stalder Jan 2005

Neural Stem Cells As A Delivery Vector For Chemokine Expression In The Central Nervous System, Mark Winston Stalder

LSU Master's Theses

Increased expression of cytokines and chemokines in the central nervous system (CNS) is closely associated with the development of retroviral-induced neurological diseases such as HIV-associated dementia, as well as other neuropathologies such as Alzheimer’s Disease and Multiple Sclerosis. The specific functions of many of these pro-inflammatory factors have yet to be elucidated in the disease process, and it is unclear whether the nature of their effects is protective, pathogenic, or both. Additionally, current models of chemokine function have inherent limitations, with direct injection resulting in a brief response that doesn’t accurately represent the effects of chronic production, and transgenic mice …

Go to article

Cytokines And Aeroallergens In The Pathogenesis Of Summer-Pasture Associated Obstructive Pulmonary Disease: Effects On Endothelin Production, Neutrophil Activation And Chemotaxis, Lais Rosa Rodrigues Costa Jan 2005

Cytokines And Aeroallergens In The Pathogenesis Of Summer-Pasture Associated Obstructive Pulmonary Disease: Effects On Endothelin Production, Neutrophil Activation And Chemotaxis, Lais Rosa Rodrigues Costa

LSU Doctoral Dissertations

Summer pasture-associated obstructive pulmonary disease (SPAOPD), a naturally occurring airway disease of horses, is characterized by clinical exacerbation associated with exposure to pasture environment during the summer. Aeroallergens are believed to trigger exacerbation of SPAOPD, cytokines are likely associated with the anamnestic response to aeroallergens, and endothelin (ET)-1 is a potential mediator of airway obstruction. The goal of this dissertation was to describe and explore the interaction of aeroallergens triggering inflammation and T lymphocytes cytokine profile with the recruitment and activation of neutrophils and synthesis of ET-1 by mononuclear leukocytes and airway epithelial cells. The temporal pattern of clinical exacerbation …

Go to article

Genetics And Functions Of The Sars Coronavirus Spike Protein, Chad Michael Petit Jan 2005

Genetics And Functions Of The Sars Coronavirus Spike Protein, Chad Michael Petit

LSU Doctoral Dissertations

The SARS-Coronavirus (SARS-CoV) is the etiological agent of the severe acute respiratory syndrome (SARS). The SARS-CoV spike (S) glycoprotein mediates membrane fusion events during virus entry and virus-induced cell-to-cell fusion. Investigations, described herein, have focused on the genetic manipulation of the SARS-CoV S glycoprotein in order to delineate functional domains within the protein. This was accomplished by incorporating single point mutations, cluster-to-lysine and cluster-to-alanine mutations, as well as carboxyl terminal truncations into the protein and investigating these mutants in transient expression experiments. Mutagenesis of either the coiled-coil domain of the S glycoprotein amino terminal heptad repeat, the predicted fusion peptide, …

Go to article

The Role Of Urease In The Pathogenesis Of Edwardsiella Ictaluri, Natha Joy Booth Jan 2005

The Role Of Urease In The Pathogenesis Of Edwardsiella Ictaluri, Natha Joy Booth

LSU Doctoral Dissertations

An Edwardsiella ictaluri strain with disruption of ureG was identified through the use of signature tagged insertion mutagenesis as being attenuated for virulence in the channel catfish host. Sequencing of the flanking regions surrounding the insert showed that the gene was part of a urease gene complex that included ureE, ureF, ureG, ureD, ureI, and an ammonium transporter homologue. The ureG gene encodes a GTP-binding accessory protein which is thought to function in energy-dependent urease assembly. The ureG mutant strain was found to be attenuated for mortality, persistence, and for the ability to establish infection in a competition challenge during …

Go to article

A Study Of The Anti-Inflammatory, Anti-Microbial And Immunomodulatory Properties Of Thalidomide In Leprosy, Azeb Tadesse Argaw Jan 2004

A Study Of The Anti-Inflammatory, Anti-Microbial And Immunomodulatory Properties Of Thalidomide In Leprosy, Azeb Tadesse Argaw

LSU Doctoral Dissertations

During the course of their disease, leprosy patients may experience two types of inflammatory reactions- erythema nodosum leprosum (ENL) or reversal reaction (RR). Thalidomide is effective treatment for ENL, but not for RR. Using concentrations of thalidomide similar to that achieved in the treatment of ENL, we investigated thalidomide’s effect on reactions, viability of M. leprae, and integrity of plasma membranes. Cells from patients with and without RR were stimulated with M. leprae (AFB), a cytosol fraction of M. leprae (MLSA) or DHAR (DHAR) antigen, and the effect of thalidomide on lymphocyte proliferation, expression of TNF-a mRNA and synthesis of …

Go to article

Improved Methods For The Isolation And Characterization Of Flavobacterium Columnare, Bradley Donovan Farmer Jan 2004

Improved Methods For The Isolation And Characterization Of Flavobacterium Columnare, Bradley Donovan Farmer

LSU Master's Theses

Columnaris disease, caused by the bacterium Flavobacterium columnare, is an economically significant problem in many warmwater fish species. Difficulties encountered in the isolation and culture of F. columnare have been an impediment to research on the organism and the disease it causes. The goal of this study was to improve the methods for isolation, culture, identification and maintenance of F. columnare. Following the evaluation of different culture media selective cytophaga agar was determined to be the optimum isolation medium, Flavobacterum columnare growth medium proved to be the optimum culture medium, and tryptone yeast extract agar with increased moisture was best …

Go to article

Evaluation Of Rough Brucella Strains As Vaccines For Brucellosis And Pseudorabies In Swine, Lorraine Harrow Molin Jan 2004

Evaluation Of Rough Brucella Strains As Vaccines For Brucellosis And Pseudorabies In Swine, Lorraine Harrow Molin

LSU Master's Theses

Brucellosis and pseudorabies lead to abortion in pregnant sows and are perpetuated by feral swine reservoirs. A multivalent oral vaccine for these diseases would improve vaccination and eradication programs worldwide. Previous studies have shown that the rough attenuated Brucella strains RB51 and VTRS1, when administered subcutaneously to swine, stimulate host immune responses, transiently colonize tissues, and provide partial protection against virulent B. suis infection in pregnant sows. A plasmid encoding for the pseudorabies virus glycoprotein D (PRV gD) has also been added to these strains as part of this project. This study evaluates the use of these strains as oral …

Go to article

Investigations Into Dna Vaccination Against Channel Catfish Virus, Heather C. Harbottle Jan 2004

Investigations Into Dna Vaccination Against Channel Catfish Virus, Heather C. Harbottle

LSU Doctoral Dissertations

The leading viral killer of commercially produced channel catfish is Channel Catfish Virus (CCV). Studies conducted to evaluate DNA vaccination against CCV compared encoded gene, dose, multiple DNA vaccines, and immune response to vaccination. Genes were selected (ORFs 1 and 3 [immediate early genes], ORFs 6, 19, and 46 [membrane genes], and ORF59 [putative major envelope glycoprotein gene], cloned into a plasmid, and expressed in mammalian and fish cell culture to detect predicted molecular weight proteins. Plasmid vaccines were injected into fish muscle in doses of 50 μg, 25 μg, 5 μg, or 1 μg and efficacy was evaluated upon …

Go to article

A Novel Strategy Of Controlling Bovine Pneumonic Pasteurellosis: Transfecting The Upper Respiratory Tract Of Cattle With A Gene Coding For The Antimicrobial Peptide Cecropin B, Charles Mitchell Boudreaux Jan 2004

A Novel Strategy Of Controlling Bovine Pneumonic Pasteurellosis: Transfecting The Upper Respiratory Tract Of Cattle With A Gene Coding For The Antimicrobial Peptide Cecropin B, Charles Mitchell Boudreaux

LSU Master's Theses

The very potent antibacterial activity of cecropin B makes it a likely candidate to prevent and/or treat Mannheimia haemolytica 1:A infection in the upper respiratory tract (URT) of cattle. The purpose of this study was to ascertain if the URT could be transfected with a gene coding for the antimicrobial peptide cecropin B. By transfecting cattle with a gene coding for cecropin B, this study attempted to inhibit colonization of a virulent strain of M. haemolytica 1:A in the URT while investigating any possible changes in the indigenous and transient nasal flora. In this study the antibacterial efficacy of cecropin …

Go to article

Estimation Of The Effect Of Misclassifications On Diagnostic Test Performance In Two Persistent Bovine Viral Infections, Kimberly Orr Jan 2004

Estimation Of The Effect Of Misclassifications On Diagnostic Test Performance In Two Persistent Bovine Viral Infections, Kimberly Orr

LSU Doctoral Dissertations

Validation of diagnostic assay performance is hampered where no gold standard exists. Bayesian estimates of the sensitivity and specificity were applied to studies of two persistent bovine infections: bovine immunodeficiency virus (BIV), and bovine viral diarrhea virus (BVDV). BVDV pathogenesis is well described. Losses of $20 million in 1992 alone led the Danish government to mandate eradication. Two enzyme-linked immunosorbent assay (ELISA) tests were developed; one antibody-based to detect exposure, one antigen-based to detect persistent infection. Bayesian estimates of the sensitivity and specificity were 96.6% and 99.2%, and 98.2% and 99.8% for the antibody and antigen ELISA, respectively. Maximum Likelihood …

Go to article

Early Tissue Migration Of And Host Response To Brugia Pahangi In Gerbils, Kristina Houpe Porthouse Jan 2004

Early Tissue Migration Of And Host Response To Brugia Pahangi In Gerbils, Kristina Houpe Porthouse

LSU Master's Theses

The host-parasite interaction during early filarial nematode migration is poorly understood. The objective of this study was to develop a model of early cutaneous filarid migration using Brugia pahangi in the jird (gerbil) host and measure the histologic and cytokine responses during this period. Male gerbils were intradermally inoculated in the left hindlimb with 100 B. pahangi L3 then necropsied at 3 hours, 24 hours, 3 days, 7 days, and 28 days post-infection. Larvae were recovered and tissues collected for histology and cytokine measurement. At 3 hours, most larvae (96.3%) were recovered from tissues associated with the infection site. Migration …

Go to article

Chromosomal Localization Of A Proinsulin Transgene Inserted With A Transposon-Based Vector Into Japanese Quail, Coturnix Coturnix, Lacey R. Mcnally Jan 2004

Chromosomal Localization Of A Proinsulin Transgene Inserted With A Transposon-Based Vector Into Japanese Quail, Coturnix Coturnix, Lacey R. Mcnally

LSU Doctoral Dissertations

The overall goals of this research were to develop a reproducible method of detecting stable DNA insertion into Japanese quail and provide a method for gene location on avian chromosomes. This research resulted in the development of a different method of obtaining chromosome spreads in Japanese quail, the establishment of primed in situ hybridization as a method for the chromosomal gene detection in birds, development of Teflon-coated coverslip slides to facilitate laser microdissection of 0.5 ƒÝm samples, and chromosomal identification of proinsulin transgene insertions by laser microdissection and nucleotide sequence from G2 Japanese quail. The 28S rDNA was found on …

Go to article

Genetics And Functions Of Herpes Simplex Virus Type 1 Membrane Proteins In Virus-Induced Cell Fusion, Virion Morphogenesis And Egress, Jeffrey Michael Melancon Jan 2004

Genetics And Functions Of Herpes Simplex Virus Type 1 Membrane Proteins In Virus-Induced Cell Fusion, Virion Morphogenesis And Egress, Jeffrey Michael Melancon

LSU Doctoral Dissertations

The Herpes Simplex Virus life cycle contains a number of membrane fusion events that must function properly to ensure a productive infection, including: virus attachment and entry into susceptible cells, de-envelopment at the outer nuclear lamellae, and virus-induced cell-to-cell fusion. A virus-free cell fusion assay was recently developed in order to attempt to understand the underlying mechanisms that are responsible for viral fusion events and was utilized in order to investigate the effect of mutations targeted to the carboxyl terminus of gB. We showed that the predicted alpha helical domain H17b within the carboxyl-terminus of gB is involved in both …

Go to article

Isolation And Characterization Of Carbonic Anhydrase From Ostertagia Ostertagi, Andrew Allan Derosa Jan 2004

Isolation And Characterization Of Carbonic Anhydrase From Ostertagia Ostertagi, Andrew Allan Derosa

LSU Doctoral Dissertations

The first event in the infection process of Ostertagia ostertagi in cattle is the process of exsheathment. Before trichostrongylid nematodes can transition from a free-living infective stage larva (L3) on pasture to a parasitic existence within the ruminant host, it must first undergo exsheathment. Exsheathment is the process whereby the L2 cuticle retained from the previous molt is cast from the L3. Exsheathment enables the developmental transition from a free-living stage on pasture to a parasitic existence in the bovine host. For those species with a predilection site in the abomasum, such as O. ostertagi, …

Go to article

First Prev Next Last