Veterinary Pathology and Pathobiology Commons^™

Detection Of Serum Antibody Responses In Cattle With Natural Or Experimental Neospora Infections, Patricia Conrad, Karen Sverlow, Mark Anderson, Joan Rowe, Robert Bondurant, Gwen Tuter, Richard Breitmeyer, California Department Of Food And Agriculture, Animal Health Branch, Mark Thurmond, Alex Ardans, J. P. Dubey, Gerarld Duhamel, Bradd Barr

School of Veterinary and Biomedical Sciences: Faculty Publications

Parasite-specific antibody responses were detected using an indirect fluorescent antibody (IFA) test in cattle that were naturally or experimentally infected with Neospora parasites. The test was developed using Neospora tachyzoites isolated from an aborted bovine fetus and grown in bovine cell cultures (isolate BPA1). In all cases, infections were confirmed by the identification of Neospora tachyzoites and/or bradyzoite cysts in fetal or calf tissues using an immunoperoxidase test procedure. Fifty-five naturally infected cows that aborted Neospora-infected fetuses had titers of 320-5,120 at the time of abortion. The titer of 6 cows that were serologically monitored over a prolonged period decreased …

Cells That Express All Five Proteins Of Vesicular Stomatitis Virus From Cloned Cdnas Support Replication, Assembly, And Budding Of Defective Interfering Particles, Asit K. Pattnaik, Gail W. Wertz

School of Veterinary and Biomedical Sciences: Faculty Publications

An alternative approach to structurefunction analysis of vesicular stomatitis virus (VSV) gene products and their interactions with one another during each phase of the viral life cycle is described. We showed previously by using the vaccinia viruslT7 RNA polymerase expression system that when cells expressing the nucleocapsid protein (N), the phosphoprotein (NS), and the large polymerase protein (L) of VSV were superinfected with defective interfering (DI) particles, rapid and efflicient replication and amplification of DI particle RNA occurred. Here, we demonstrate that all five VSV proteins can be expressed simultaneously when cells are cotransfected with plasmids containing the matrix protein …

Monoclonal Antibodies To The Fusion Protein Of Bovine Respiratory Syncytial Virus, Kent M. Mulkey, Gary A. Anderson

School of Veterinary and Biomedical Sciences: Faculty Publications

Five monoclonal antibodies specific for bovine respiratory syncytial virus were characterized by Western immunoblotting, radioimmunoprecipitation, and epitope mapping assays. The monoclonal antibodies were found to be specific for the fusion protein, and there were at least two antigen binding sites, one of which was neutralizing.

Replication And Amplification Of Defective Interfering Particle Rn As Of Vesicular Stomatitis Virus In Cells Expressing Viral Proteins From Vectors Containing Cloned Cdnas, Asit K. Pattnaik, Gail W. Wertz

School of Veterinary and Biomedical Sciences: Faculty Publications

Replication and amplification of RNA genomes of defective interfering (DI) particles of vesicular stomatitis virus (VSV) depend on the expression of viral proteins and have untD now been attained only in ceUs coinfected with helper VSV. In the work described in this report, we used a recombinant vaccinia virus-T7 RNA polymerase expression system to synthesize individual VSV proteins in cells transfected with plasmid DNAs that contain cDNA copies of the VSV genes downstream of the T7 RNA polymerase promoter. In this way, we were able to examine the ability of VSV proteins, individually and in combination, to support DI particle …

The Live Sheep Export Industry, Richard Norris, Barry Richards, Tony Higgs

Journal of the Department of Agriculture, Western Australia, Series 4

Australia's live sheep trade to Middle Eastern countries is an important export industry. However, about 2 per cent of the sheep die, mainly during the sea voyage. The Western Australian Department of Agriculture has conducted a major research programme to improve the health and welfare of sheep during live export. The programme has'examined many aspects of the export sheep industry, and a substantial part of it has focused on why sheep die during sea transport.

Efficacy Of A Cell Extract From Actinobacillus (Haemophilus) Pleuropneumoniae Serotype 1 Against Disease In Swine, P. J. Fedorka-Cray, M.J. Huether, D.L. Stine, G. A. Anderson

School of Veterinary and Biomedical Sciences: Faculty Publications

We partially characterized a cell extract (CE) from Actinobacillus pleuropneumoniae serotype 1 and used the CE to test the efficacy of secreted proteins against disease. Secreted products from 4-h culture supernatants were precipitated with 20% polyethylene glycol. Analysis of the CE indicated the presence of protein, endotoxin, and carbohydrate. Hemolytic activity to bovine erythrocytes and cytotoxic activity to porcine mononuclear leukocytes was also demonstrated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the CE from a 4-h culture showed a major band at 110 kilodaltons (kDa), while a CE of a 26-h culture indicated the presence of a number of additional …

Improved Selective Medium For The Isolation Of Treponema Hyodysenteriae, Robert A. Kunkle, J. M. Kinyon

School of Veterinary and Biomedical Sciences: Faculty Publications

An agar medium with improved selection for Treponema hyodysenteriae was developed. Cultures of T. hyodysenteriae and T. innocens, feces from 11 clinically normal pigs, and colonic contents from 6 pigs with gross lesions consistent with swine dysentery were diluted in phosphate-buffered saline and plated on Trypticase soy agar (BBL Microbiology Systems, Cockeysville, Md.) with 5% citrated bovine blood (TSA), TSA with 400 ,ug of spectinomycin per ml (TSA-S400), TSA-S400 with 25 ,ug each of colistin and vancomycin per ml, and TSA with 5% pig feces extract and five antimicrobial agents (spiramycin, rifampin, vancomycin, colistin, and spectinomycin) (BJ). Viable numbers …

Formation Of Influenza Virus Particles Lacking Hemagglutinin On The Viral Envelope, Asit K. Pattnaik, Donald J. Brown, Debi P. Nayak

School of Veterinary and Biomedical Sciences: Faculty Publications

We investigated the intraceUular block in the transport of hemagglutinin (HA) and the role of HA in virus particle formation by using temperature-sensitive (Is) mutants (1s134 and 1s61S) of inOuenza virus AlWSN/33. We found that at the nonpermissive temperature (39.5°C), the exit of ts HA from the rough endoplasmic reticulum to the Golgi complex was blocked and that no additional block was apparent in either the exit from the Golgi complex or post-Golgi complex transport. When MDBK ceUs were infected with these mutant viruses, they produced noninfectious virus particles at 39.5°C. The efficiency of particle formation at 39.5°C was essentiaUy …

Identification Of Four Complementary Rna Species In Akabane Virus-Infected Cells, Asit K. Pattnaik, G. Abraham

School of Veterinary and Biomedical Sciences: Faculty Publications

The analysis of RNA extracted from purified Akabane virus demonstrated the presence of three size classes of single-stranded RNAs with sedimentation coefficients of 31S (large, L), 26S (medium, M), and 13S (small, S). Molecular weights of these RNA species were estimated to be 2.15 x 10⁶,1.5 X 10⁶, and 0.48 x 10⁶ for the L, M, and S RNAs, respectively. Hybridization analysis involving viral genomic RNA and RNA from virus-infected cells resulted in the identification of four virus-specific cRNA species in infected cells. These cRNAs were found to be nonpolyadenylated by their inability to bind …

Infectious Pancreatic Necrosis (Ipn) Of Salmonid Fishes, Phillip E. Mcallister

United States Fish and Wildlife Service: Publications

Infectious pancreatic necrosis (IPN) is a viral disease principally associated with salmonids, although IPN and lPN-like viruses have been isolated from various nonsa1rnonid fish and marine invertebrates. Acute infection occurs in 1- to 4-month-old salmonids and can result in cumulative mortality approaching 100%. In contrast, fish 6 months old or older can undergo subclinical or inapparent infection, but experience no significant mortality. Survivors of the disease can become lifelong carriers of the virus. Epizootics of IPN have been reported worldwide, and several virus serotypes are recognized. The disease known as IPN was probably first described by M'Gonigle (1941) as acute …

Some Basic Facts About Primary Blowflies, N Monzu

Journal of the Department of Agriculture, Western Australia, Series 4

Research has begun on blowfly species attacking sheep in Western Australia. Already this research has indicated the importance of a species in Western Australia which is not a problem in the Eastern States.

Future management of sheep flocks to minimise fly strike may require recognition of the differences between blowfly species. This article gives a description of the primary species - those which can initiate strikes.

Parasites Of Channel Catfish In Illinois Hatcheries, Charles Dale Meryman

Masters Theses

Ninety channel catfish (Ictalurus punctatus) were trapped during September 1973 through April 1974 at three hatcheries located at Worden, Centralia, and Kinmundy, Illinois. All were examined for parasites and 87 were parasitized. The trematodes Azygia angusticauda and Neascus sp. were found in the gastrointestinal system and Cleidodiscus floridanus and Dactylogyrus sp. were attached to the gills. Five cestode genera, including Bothriocephalus sp., Corallobothrium giganteum, Eubothrium sp., Haplobothrium sp., and Proteocephalus ambloplitis were present. Rhabdochona sp. was the only nematode found. Protozoans; Costia sp., Myxobolus sp., Scyphidia macropodia and Trichodina discoidea and one kind of glochidium were observed. …

Cancer Eye Of Cattle, M R. Gardiner, J. L. Anderson, D. E. Robertson

Journal of the Department of Agriculture, Western Australia, Series 4

Cancer of the eye and eyelids is mainly a disease of cattle, although it occasionally occurs in most species of animals and in man. It is the most common cancer in cattle, especially in certain breeds.

Sheep Fertility In Western Australia, R J. Lightfoot

Journal of the Department of Agriculture, Western Australia, Series 4

Why do one-quarter of the ewes that are joined in W.A. fail to lamb? Sheep and Wool Adviser Dr. R. J. Lightfoot analyses the eight stages in sheep reproduction, explaining the causes and possible prevention of infertility at each stage.

Effects Of Mastitis On Milk Yield And Composition, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

EVERY DAIRY farmer knows how much he spends on the treatment of cows affected with mastitis, but the actual loss in production is more difficult to estimate.

As part of the current mastitis research programme the Department of Agriculture's Animal and Dairying Divisions surveyed 37 dairy herds with a believed high incidence of mastitis.

Submitting Plants For Disease Identification, M D. Marcley

Journal of the Department of Agriculture, Western Australia, Series 4

THERE are several important points to remember when submitting plant material for disease identification by the Department of Agriculture's Plant Pathology Branch.

Can Mastitis Be Eliminated?, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

Can mastitis be eliminated from a dairy herd? The results of a 12-month continuous study of four commercial herds show that it could be done.

Eye Cancer In Cattle, E P. Meyer

Journal of the Department of Agriculture, Western Australia, Series 4

THE CONDITION known as eye cancer occurs in all ages and breeds of cattle but is most common in certain strains of the Hereford breed that have non-pigmented or mottled eyes and eyelids.

A yearly incidence of up to 5 per cent can occur in susceptible herds.

Lamb Mortality On Agricultural Research Stations, K P. Croker

Journal of the Department of Agriculture, Western Australia, Series 4

Progress report of a study of lamb losses in experimental flocks on Department of Agriculture research stations in the agricultural areas.

THE major barrier to increased production by the West Australian sheep and wool industry is the shortage of sheep in the agricultural areas of the State, and much research has been directed towards overcoming this shortage.

Swine Erysipelas, G C De Chaneet

Journal of the Department of Agriculture, Western Australia, Series 4

SWINE ERYSIPELAS, first diagnosed in Western Australia in 1960, has become a source of considerable loss to the pig industry.

Most losses have resulted from carcass condemnation at abattoirs because of arthritis caused by the erysipelas organism.

Sheep Breeding Problems In W.A. : The Industry's View, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

IN 1963 a short questionnaire was distributed to farmers and pastoralists seeking information about sheep breeding problems and lamb losses.

This was a fore-runner to the Department of Agriculture survey into the causes of abortion and lamb mortality which is reported elsewhere in this issue.

Time Of Lambing At Esperance, R J. Doyle

Journal of the Department of Agriculture, Western Australia, Series 4

ESPERANCE is one of the few places in Western Australia at which experiments over three years have shown a definite improvement in number of lambs produced to marking, as a result of lambing in the August-September period.

How To Care For Orphan Lambs, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

WITH the current high price and shortage of sheep in W.A., farmers might consider the bother of artificially rearing orphan lambs more worthwhile than before.

More Light On Lamb Losses : Third And Final Report Of A Survey Of Lamb Mortalities In W.A, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

DURING the past three years the Department of Agriculture's Animal Health Laboratory carried out an investigation into the causes of perinatal lamb losses.

This article records the results of this work.

Congenital Abnormalities In Sheep In Western Australia, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

IN every flock of sheep an abnormal or deformed lamb is seen from time to time.

But in some flocks in some years the incidence of these abnormal lambs is higher than usual.

Urinary Calculus Disease Of Sheep In Western Australia, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

THE formation of concretions in the urinary tract of rams and wethers is one of the most persistent causes of loss of sheep in Western Australia, and has been so for many years.

Dead Lambs : A Dead Loss, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

During the past year the Animal Health Laboratory has continued its survey into the causes of abortion in sheep and perinatal lamb losses.

This article records the results of this work.

LAMBS and specimens from lambs were submitted to the Animal Health Laboratory from 229 properties situated in 121 areas in this survey during 1964.

Post-mortem examination was carried out on 1,422 lambs and specimens from 61 lambs were also examined.

Calfhood Diseases. 1. Gastro-Intestinal Diseases, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

CALFHOOD disease may be divided for convenience into three main categories:

• Those affecting the gastro-intestinal tract, including diseases of an infectious nature and those arising as a result of infestation by worms (parasitism).

• Those affecting the respiratory system, including lungworms.

• Those resulting from nutritional deficiencies, especially vitamins and minerals.

Why Did These Lambs Die?, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

INVESTIGATIONS over the past two years have shown that most lamb deaths in Western Australia occur during the first three days of life, that is, in the critical neonatal period.

Most of the lambs died between 24 and 72 hours after birth.

Congenital Abnormalities In Sheep, Stanley M. Dennis

Journal of the Department of Agriculture, Western Australia, Series 4

More than 60 forms of abnormality have been found in lambs in Western Australia and in some flocks the proportion of particular abnormalities has been as high as 8 per cent, of lambs born.

In this article Dr. Dennis discusses the causes of abnormalities and reports progress of a Statewide survey of congenital abnormalities.