Small or Companion Animal Medicine Commons^™

Long-Term Clinical Outcome Of Corneal Endothelial Degeneration Managed With Keratoleptynsis In A Chihuahua Dog, Manbok Jeong

The Thai Journal of Veterinary Medicine

A 9-year-old spayed female Chihuahua presented with a 2-month history of progressive corneal edema in the left eye. Slit lamp biomicroscopy showed moderate corneal edema, instability of the axial corneal epithelial surface, and an anterior cortical opacity of the lens. Based on these results, a diagnosis of canine endothelial degeneration was made with the incidental finding of an incipient cataract in the left eye. Keratoleptynsis successfully managed the corneal edema for 28 months after surgery without corneal ulceration. This case study underscores the need for an early surgical approach using keratoleptynsis for dogs with a progressive nature of canine endothelial …

Molecular Mechanisms Of Suberoylanilide Hydroxamic Acid In The Inhibition Of Tgf-Β1-Mediated Canine Corneal Fibrosis, Kristina M. Gronkiewicz, Elizabeth A. Giuliano, Ajay Sharma, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—To investigate molecular mechanisms mediating anti-fibrotic effect of SAHA in the canine cornea using an in vitro model. We hypothesized that SAHA attenuates corneal fibrosis by modulating Smad-dependent and, to a lesser extent, Smad-independent signaling pathways activated by TGF-β1, as well as matrix metalloproteinase (MMP) activity.

Methods—Cultured canine corneal fibroblasts (CCF) were incubated in the presence/absence of TGF-β1 (5ng/ml) and SAHA (2.5μM) for 24hrs. Western blot analysis was used to quantify non-phosphorylated and phosphorylated isoforms of Smad2/3, p38 MAP kinase (MAPK), ERK1/2 and JNK1. Real-time PCR and zymography were utilized to quantify MMP1, MMP2, MMP8 and MMP9 mRNA expression and …