Other Veterinary Medicine Commons^™

African Swine Fever, Vienna R. Brown, Julianna B. Lenoch, Courtney F. Bowden

United States Department of Agriculture Wildlife Services: Staff Publications

At the time of writing this chapter, a global pandemic of African swine fever (ASF) is ongoing with the virus having moved from Eastern Europe, Asia, and into the Caribbean—leaving swine production in devastation along the way. Due to the global spread of African swine fever virus (ASFV), the persistence of the virus, and the increasing number of endemic countries, this disease poses an imminent threat of introduction into North America and other countries that are currently ASF free. Throughout the chapter, we reference Eurasian wild boar (Sus scrofa) which are charismatic megafauna that are native to Europe and Asia. …

Spatially Structured Brown-Headed Cowbird Control Measures And Their Effects On Kirtland’S Warbler Long-Term Population Sustainability, Eric A. Margenau, Nathan W. Cooper, Donald J. Brown, Deahn M. Donner, Peter P. Marra, Pat Ryan

United States Department of Agriculture Wildlife Services: Staff Publications

Context: Brown-headed cowbirds (Molothrus ater), through brood parasitism, can exert extrinsic population growth pressures on North American songbirds. Cowbird removal programs may reduce parasitism rates on host species but can be expensive and difficult to implement throughout a host species’ breeding range.

Aim: We estimated cowbird abundance and nest parasitism rates within Kirtland’s warbler (Setophaga kirtlandii) primary breeding range in Michigan, USA, and determined the maximum sustainable parasitism rate for Kirtland’s warblers under several spatially structured cowbird removal designs.

Methods: We conducted point counts to estimate cowbird abundance and monitored nests to quantify nest parasitism rates …

Willingness To Pay For Reintroducing Wolves In A Divided Voting Base, Dana Lk Hoag, Jesse Burkhardt, Benjamin Ghasemi, Stewart W. Breck, Rebecca Niemiec, Kevin Crooks

United States Department of Agriculture Wildlife Services: Staff Publications

Wolves will soon be reintroduced in Colorado based on a statewide ballot initiative that narrowly passed in November 2020. Using an economic choice experiment, we estimate the benefits that wolf introduction might bring to Colorado. We calculated willingness to pay (WTP) for a sustainable wolf population by considering six program attributes: 1) state wolf population, 2) compensation for livestock-related losses, 3) cost-sharing for conflict reduction, 4) number of livestock killed statewide, 5) lethal government control of wolves, and 6) wolf hunting. Respondents who reported they voted yes on the ballot initiative had a positive WTP for a population of 200 …

Can Demographic Histories Explain Long-Term Isolation And Recent Pulses Of Asymmetric Gene Flow Between Highly Divergent Grey Fox Lineages?, Sophie Preckler-Quisquater, Elizabeth M. Kierepka, Dawn M. Reding, Antoinette J. Piaggio, Benjamin N. Sacks

United States Department of Agriculture Wildlife Services: Staff Publications

Secondary contact zones between deeply divergent, yet interfertile, lineages provide windows into the speciation process. North American grey foxes (Urocyon cinereoargenteus) are divided into western and eastern lineages that diverged approximately 1 million years ago. These ancient lineages currently hybridize in a relatively narrow zone of contact in the southern Great Plains, a pattern more commonly observed in smaller-bodied taxa, which suggests relatively recent contact after a long period of allopatry. Based on local ancestry inference with whole-genome sequencing (n = 43), we identified two distinct Holocene pulses of admixture. The older pulse (500–3500 YBP) reflected unidirectional …

Investigation Of Risk Factors For Introduction Of Highly Pathogenic Avian Influenza H5n1 Infection Among Commercial Turkey Operations In The United States, 2022: A Case-Control Study, Kelly A. Patyk, Victoria L. Fields, Andrea L. Beam, Matthew A. Branan, Rachel E. Mcguigan, Alice Green, Mia K. Torchetti, Kristina Lantz, Alexis Freifeld, Katherine Marshall, Amy H. Delgado

United States Department of Agriculture Wildlife Services: Staff Publications

Introduction: The 2022–2023 highly pathogenic avian influenza (HPAI) H5N1 outbreak in the United States (U.S.) is the largest and most costly animal health event in U.S. history. Approximately 70% of commercial farms affected during this outbreak have been turkey farms. Methods: We conducted a case-control study to identify potential risk factors for introduction of HPAI virus onto commercial meat turkey operations. Data were collected from 66 case farms and 59 control farms in 12 states. Univariate and multivariable analyses were conducted to compare management and biosecurity factors on case and control farms. Results: Factors associated with increased risk of infection …

Outbreak Of Epizootic Hemorrhagic Disease In Captive Reindeer (Rangifer Tarandus), Emma H. Torii, Arno Wünschmann, Mia Kim Torchetti, Leo Koster, Albert Van Geelen, Randy Atchison, Anne Rivas

United States Department of Agriculture Wildlife Services: Staff Publications

In September 2020, an outbreak of epizootic hemorrhagic disease occurred in captive reindeer (Rangifer tarandus) and was associated with neurological signs and mortality. Four reindeer died or were euthanized after acute illness over a 12-day period. Affected reindeer displayed abnormal behavior, neurologic signs, lethargy, and/or lameness. The most consistent gross finding was dark red streaks throughout the adrenal gland cortices (4/4). One animal had acute hemorrhage involving the subcutis and skeletal muscles over the ventrolateral body wall and back, and abomasal serosa. Histologically, the most common lesions were adrenal gland cortical hemorrhage (4/4) with necrosis (3/4) and lymphoplasmacytic meningoencephalitis with …

Investigation Of Risk Factors For Introduction Of Highly Pathogenic Avian Influenza H5n1 Virus Onto Table Egg Farms In The United States, 2022: A Case–Control Study, Alice L. Green, Matthew Branan, Victoria L. Fields, Kelly Patyk, Stephanie K. Kolar, Andrea Beam, Katherine Marshall, Rachel Mcguigan, Matthew Vuolo, Alexis Freifeld, Mia Kim Torchetti, Kristina Lantz, Amy H. Delgado

United States Department of Agriculture Wildlife Services: Staff Publications

Introduction: The 2022–2023 highly pathogenic avian influenza (HPAI) H5N1 outbreak in the United States (U.S.) is the most geographically extensive and costly animal health event in U.S. history. In 2022 alone, over 57 million commercial and backyard poultry in 47 U.S. states were affected. Over 75% of affected poultry were part of the commercial table egg production sector. Methods: We conducted a case–control study to identify potential risk factors for introduction of HPAI virus onto commercial table egg operations. Univariate and multivariable analyses were conducted to compare farm characteristics, management, and biosecurity factors on case and control farms. Results: Factors …

Rabies Virus Serosurvey Of The Small Indian Mongoose (Urva Auropunctata) Across Multiple Habitats In Puerto Rico, 2014–21, Are R. Berentsen, Mel J. Rivera-Rodriguez, Fabiola B. Torres-Toledo, Amy J. Davis, Richard Chipman, Amy Gilbert

United States Department of Agriculture Wildlife Services: Staff Publications

The small Indian mongoose (Urva auropuncata) is a rabies reservoir in Puerto Rico and accounts for over 70% of reported animal rabies cases annually. The presence of rabies virus-neutralizing antibodies (RVNA) is often used as a tool to measure exposure to rabies virus in wildlife populations. We conducted a serosurvey of mongooses at 11 sites representing six habitat types across Puerto Rico. We collected a serum sample from 464 individual mongooses during 2014–2021. Overall, 80/464 (17.0%; 95% confidence interval, 14.1–20.9%; 55 male, 23 female, and two sexes not recorded) of individual mongooses sampled across all habitats were RVNA …

Evaluation Of Oral Baits And Distribution Methods For Tasmanian Devils (Sarcophilus Harrisii), Sean Dempsey, Ruth J. Pyer, Amy Gilbert, Nicholas M. Fountain-Jones, Jennifer M. Moffat, Sarah Benson-Amram, Timothy J. Smyser, Andrew S. Flies

United States Department of Agriculture Wildlife Services: Staff Publications

Context

Diseases are increasingly contributing to wildlife population declines. Tasmanian devil (Sarcophilus harrisii) populations have locally declined by 82%, largely owing to the morbidity and mortality associated with two independent transmissible devil facial tumours (DFT1 and DFT2). Toxic baits are often used as a management tool for controlling vertebrate pest populations in Australia, but in other areas of the world, oral baits are also used to deliver vaccines or pharmaceuticals to wildlife.

Aim

Our goal was to evaluate the potential use of edible baits as vehicles for vaccine delivery to Tasmanian devils.

Method

We first tested bait palatability with …

Predicting Dispersal And Conflict Risk For Wolf Recolonization In Colorado, Mark A. Ditmer, George Wittemyer, Katherine A. Zeller, Stewart W. Breck, Robert J. Fletcher Jr., Kevin R. Crooks

United States Department of Agriculture Wildlife Services: Staff Publications

1. The colonization of suitable yet unoccupied habitat due to natural dispersal or human introduction can benefit recovery of threatened species. Predicting habitat suitability and conflict potential of colonization areas can facilitate conservation planning.

2. Planning for reintroduction of gray wolves (Canis lupus) to the United States state of Colorado is underway. Assessing which occupancy sites minimize the likelihood of human-wolf conflict during dispersal events and seasonal movements is critical to the success of this initiative.

3. We used a spatial absorbing Markov chain (SAMC) framework, which extends random walk theory and probabilistically accounts for both movement behavior …

Development And Evaluation Of Prototype Toxicant-Delivery Bait Stations For The Control Of The Small Indian Mongoose, Carmen C. Antaky, Robert T. Sugihara, Israel L. Leinbach, Shane R. Siers, Emily W. Ruell, Steven C. Hess

United States Department of Agriculture Wildlife Services: Staff Publications

We conducted research to develop a safe and effective toxic bait to control the small Indian mongoose (Urva auropunctata), an invasive vertebrate predator impacting the survival of native species in Hawai‘i (United States) and in other parts of the world. A preserved fish-based bait product was found to be highly palatable to mongooses in cage trials and subsequent formulations with diphacinone (0.005%) showed promise as an efficacious toxic bait for mongooses. This product is intended for future use to control mongooses in conservation and urban areas, and as a biosecurity tool at ports of entry to address accidental …

Assessment Of Spilled Toxic Bait By Wild Pigs And Potential Risk To Non-Target Species, John C. Kinsey, Justin A. Foster, Nathan P. Snow, Jason D. Wishart, Linton D. Staples, Janis K. Bush, Kurt V. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Background

In 2018, a sodium nitrite (SN)-based toxic bait for invasive wild pigs (hereafter wild pigs; Sus scrofa), was evaluated to determine its effectiveness in reducing local wild pig populations in Texas. Localized population reductions of > 70% were achieved, but spillage of bait outside wild pig-specific feeders (bait stations) caused by feeding wild pigs resulted in the deaths of non-target animals. To evaluate risks to non-target animals, we tested whether bait presentation influenced the total amount of bait spilled by wild pigs and estimated the associated risk to non-target species.

Results

We found that bait spilled outside bait stations …

Population Density Of The Small Indian Mongoose (Urva Auropunctata) Across Multiple Habitat Types And Seasons In Puerto Rico, Are R. Berentsen, Caroline C. Sauvé, Mel J. Rivera-Rodriguez, Fabiola B. Torres-Toledo, Richard B. Chipman, Amy T. Gilbert

United States Department of Agriculture Wildlife Services: Staff Publications

The small Indian mongoose (Urva auropunctata) is a rabies reservoir on several Caribbean Islands including Puerto Rico. In the continental United States, oral rabies vaccination (ORV) has been used to control and locally eliminate rabies viruses targeting meso-carnivores including raccoons (Procyon lotor), grey foxes (Urocyon cinereoargenteus), and coyotes (Canis latrans), and has more recently been proposed to mitigate and control mongoose rabies in Puerto Rico. A fundamental understanding of the population density of the target species is an important factor in planning bait application rates prior to ORV operations. In Puerto Rico, …

Comparison Of Daily Activity Patterns Across Seasons Using Gps Telemetry And Camera Trap Data For A Widespread Mammal, David W. Wolfson, Peter E. Schlichting, Raoul K. Boughton, Ryan S. Miller, Kurt C. Vercauteren, Jesse S. Lewis

United States Department of Agriculture Wildlife Services: Staff Publications

Daily activity patterns of animals can be dynamic across seasons in response to changing environmental conditions. Daily activity, though, has rarely been evaluated in relation to multiple factors (for example, seasons, demographics, and data collection methods), which could be important for understanding what drives activity patterns. Here, we evaluated the daily activity patterns of a widespread invasive species, wild pigs (Sus scrofa), across two ecologically different study areas at Buck Island Ranch, Florida, and Tejon Ranch, California (United States), from 2015 to 2018. Using GPS telemetry data (62 individuals in Florida, 21 individuals in California) and detections from …

Are Rabid Raccoons (Procyon Lotor) Ready For The Rapture? Determining The Geographic Origin Of Rabies Virus-Infected Raccoons Using Radcapture And Microhaplotypes, Matthew W. Hopken, Antoinette J. Piaggio, Zaid Abdo, Richard B. Chipman, Clara P. Mankowski, Kathleen M. Nelson, Mikaela Samsel Hilton, Christine Thurber, Mirian T. N. Tsuchiya, Jesús E. Maldonado, Amy T. Gilbert

United States Department of Agriculture Wildlife Services: Staff Publications

North America is recognized for the exceptional richness of rabies virus (RV) wildlife reservoir species. Management of RV is accomplished through vaccination targeting mesocarnivore reservoir populations, such as the raccoon (Procyon lotor) in eastern North America. Raccoons are a common generalist species, and populations may reach high densities in developed areas, which can result in contact with humans and pets with potential exposures to the raccoon variant of RV throughout the eastern United States. Understanding the spatial movement of RV by raccoon populations is important for monitoring and refining strategies supporting the landscape-level control and local elimination of …

Home Range Overlap Between Small Indian Mongooses And Free Roaming Domestic Dogs In Puerto Rico: Implications For Rabies Management, Caroline C. Sauvé, Are R. Berentsen, Steven F. Llanos, Amy T. Gilbert, Patrick A. Leighton

United States Department of Agriculture Wildlife Services: Staff Publications

The small Indian mongoose (Urva auropunctata) is the primary terrestrial wildlife rabies reservoir on at least four Caribbean islands, including Puerto Rico. In Puerto Rico, mongooses represent a risk to public health, based on direct human exposure and indirectly through the transmission of rabies virus to domestic animals. To date, the fundamental ecological relationships of space use among mongooses and between mongooses and domestic animals remain poorly understood. This study is the first to report mongoose home range estimates based on GPS telemetry, as well as concurrent space use among mongooses and free roaming domestic dogs (FRDD; Canis …

Type And Frequency Of Wild Pig-Domestic Livestock Contacts And Operator Concern For Disease Spread, Sophie C. Mckee, Daniel F. Mooney, Ryan S. Miller

United States Department of Agriculture Wildlife Services: Staff Publications

Wild pigs (Sus scrofa) can levy substantial costs to domestic livestock operations and global supply chains due to disease introduction. Producers can take defensive action to avert these costs, but the factors influencing these decisions are not well understood. We examined survey data to characterize the type and frequency of contacts between wild pigs and domestic livestock and investigated the determinants of farm operator concern for disease introduction using an ordered logit model. We found that operators with livestock kept in buildings were more concerned about disease than those with livestock kept in fenced areas or that roam …

Turkey Vulture Survival Is Reduced In Areas Of Greater Road Density, Adrián Naveda-Rodríguez, Keith L. Bildstein, David R. Barber, Jean-François Therrien, Michael L. Avery, Bryan M. Kluever, Scott A. Rush, Francisco J. Vilella

United States Department of Agriculture Wildlife Services: Staff Publications

The demography of, and factors that influence these metrics, are largely unknown for most vultures in the Americas. Survivorship of turkey vultures (Cathartes aura) may be influenced by landscape heterogeneity and human disturbance. We quantified the effects of landscape composition (Shannon’s diversity index) and configuration (contagion, edge density, and largest patch index), and human disturbance (road density) on the annual and seasonal survival probabilities of the three North American breeding populations (western, central, and eastern) of turkey vultures that spend the nonbreeding season in the southeastern portion of the Nearctic and the northern Neotropics during a 17-year period. …

Hepatozoon Spp. Infection In Wild Canids In The Eastern United States, Eliza Baker, Alex Clemson, Debra Miller, Kayla Buck Garrett, Christopher A. Cleveland, Justin Brown, Kyle Van Why, Richard W. Gerhold Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

Background Hepatozoon spp. are apicomplexan parasites known to cause musculoskeletal disease in a variety of animals. Two species are known to infect wild and domestic canids in the United States: Hepatozoon canis and H. americanum.

Methods In this study, blood, heart, and/or spleen samples were collected from 278 wild canids (180 coyotes, 93 red foxes, and 5 gray foxes) in the eastern US and tested via PCR for Hepatozoon. Histology slides of heart and skeletal muscle were assessed for Hepatozoon cysts and associated inflammation when fresh tissue was available (n = 96).

Results Hepatozoon spp. were …

A Next-Generation Sequencing Study Of Arthropods In The Diet Of Laysan Teal Anas Laysanensis, Wieteke A. Hothuijzen, Carmen C. Antaky, Elizabeth N. Flint, Jonathan H. Plissner, Coral A. Wolf, Holly P. Jones

United States Department of Agriculture Wildlife Services: Staff Publications

The critically endangered Laysan teal Anas laysanensis (known as koloa po¯haka in the Hawaiian language), in the northwestern Hawaiian Islands has wild populations on Kamole (Laysan Island), Kuaihelani (Midway Atoll NWR) and Ho¯laniku¯ (Kure Atoll). The Laysan teal face a new risk on Sand Island in Kuaihelani: non-target poisoning via a pending House Mouse Mus musculus eradication program. After mice were observed attacking and depredating Laysan albatross Phoebastria immutabilis (mo¯lı¯) in 2015, plans to eradicate the mice were developed to protect this seabird species. This approach, however, risks poisoning the Laysan teal. To reduce exposure, teal will be translocated during …

Raccoon Densities Across Four Land Cover Types In The Southeastern United States, Jocab E. Hill, James L. Helton, David A. Bernasconi, Wesley C. Dixon, Matt T. Hamilton, Richard B. Chipman, Amy T. Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

Raccoons (Procyon lotor) are the primary reservoir for rabies virus in eastern North America. Management of rabies in raccoons is achieved primarily with the use of oral rabies vaccination (ORV) and effective ORV bait densities are determined in part by the densities of raccoons. Decisions regarding ORV bait densities, however, are limited by an incomplete understanding of raccoon densities across the spectrum of landscapes they occupy. We carried out a markrecapture study of raccoons on the Savannah River Site in South Carolina, USA, from 2017–2019, to develop sex‐ and landscape‐specific raccoon density estimates across 4 rural land cover …

Secondary Traumatic Stress Is An Occupational Hazard For Pet Hospice Support Staff: Psychoeducational Support Groups Provide The Cure, Kari Hayden

DSW Capstone Projects

There are three separate yet interrelated products presented in this final capstone product. The completed product is focused on pet hospice veterinary support staff and the need for an evidence-based social work intervention to combat secondary traumatic stress. This capstone uses the andragogical approach to adult learning theory as the foundation to construct a psychoeducational peer support group specific to this population. Implications for clinical social work in veterinary medicine are presented within this project.

The first scholarly product presented is the Systematic Literature Review (SLR). The SLR utilizes a scoping review process in an attempt to locate prior research …

Exposure Of Egyptian Rousette Bats (Rousettus Aegyptiacus) And A Little Free-Tailed Bat (Chaerephon Pumilus) To Alphaviruses In Uganda, Rebekah C. Kading, Erin M. Borland, Eric C. Mossel, Teddy Nakayiki, Betty Nalikka, Jeremy P. Ledermann, Mary B. Crabtree, Nicholas A. Panella, Luke Nyakarahuka, Amy T. Gilbert, Julian C. Kerbis-Peterhans, Jonathan S. Towner, Brian R. Amman, Tara K. Sealy, Barry R. Miller, Julius J. Lutwama, Robert M. Kityo, Ann M. Powers

United States Department of Agriculture Wildlife Services: Staff Publications

The reservoir for zoonotic o’nyong-nyong virus (ONNV) has remained unknown since this virus was first recognized in Uganda in 1959. Building on existing evidence for mosquito bloodfeeding on various frugivorous bat species in Uganda, and seroprevalence for arboviruses among bats in Uganda, we sought to assess if serum samples collected from bats in Uganda demonstrated evidence of exposure to ONNV or the closely related zoonotic chikungunya virus (CHIKV). In total, 652 serum samples collected from six bat species were tested by plaque reduction neutralization test (PRNT) for neutralizing antibodies against ONNV and CHIKV. Forty out of 303 (13.2%) Egyptian rousettes …

Divergent Serpentoviruses In Free-Ranging Invasive Pythons And Native Colubrids In Southern Florida, United States, Steven B. Tillis, Jillian M. Josimovich, Melissa A. Miller, Laura L. Hoon-Hanks, Arik M. Hartmann, Natalie M. Claunch, Marley E. Iredale, Tracey D. Logan, Amy A. Yackel Adams, Ian A. Bartoszek, John S. Humphrey, Bryan M. Kluever, Mark D. Stenglein, Robert N. Reed, Christina M. Romagosa, James F.X. Wellehan, Robert J. Ossiboff

United States Department of Agriculture Wildlife Services: Staff Publications

Burmese python (Python bivittatus) is an invasive snake that has significantly affected ecosystems in southern Florida, United States. Aside from direct predation and competition, invasive species can also introduce nonnative pathogens that can adversely affect native species. The subfamily Serpentovirinae (order Nidovirales) is composed of positive-sense RNA viruses primarily found in reptiles. Some serpentoviruses, such as shingleback nidovirus, are associated with mortalities in wild populations, while others, including ball python nidovirus and green tree python nidovirus can be a major cause of disease and mortality in captive animals. To determine if serpentoviruses were present in invasive Burmese …

A Novel Vaccine Candidate Against Rabbit Hemorrhagic Disease Virus 2 (Rhdv2) Confers Protection In Domestic Rabbits, Angela M. Bosco-Lauth, Bethany Cominsky, Stephanie Porter, J. Jeffrey Root, Amber Schueler, Gary Anderson, Sara Vanderwal, Andy Benson

United States Department of Agriculture Wildlife Services: Staff Publications

OBJECTIVE To evaluate efficacy of a novel vaccine against rabbit hemorrhagic disease virus 2 (RHDV2) in domestic rabbits.

ANIMALS 40 New Zealand White rabbits obtained from a commercial breeder.

PROCEDURES Rabbits were vaccinated and held at the production facility for the duration of the vaccination phase and transferred to Colorado State University for challenge with RHDV2. Rabbits were challenged with oral suspensions containing infectious virus and monitored for clinical disease for up to 10 days. Rabbits that died or were euthanized following infection were necropsied, and livers were evaluated for viral RNA via RT-PCR.

RESULTS None of the vaccinated animals …

Seasonal Habitat Selection By American White Pelicans, Frederick L. Cunningham, Guiming Wang, D. Tommy King

United States Department of Agriculture Wildlife Services: Staff Publications

Resource utilization strategies of avian migrants are a major concern for conservation and management. Understanding seasonal habitat selection by migratory birds helps us explain the ongoing continental declines of migratory bird populations. Our objective was to compare the secondorder and third-order habitat selection by the American White Pelican (Pelecanus erythrorhynchos; hereafter pelican) between the breeding and non-breeding grounds. We tested the Lack hypothesis that habitat selection by migratory birds is stronger on the breeding grounds than on the nonbreeding grounds. We used random-effect Dirichlet-multinomial models to estimate the second-order habitat selection between the seasons with the GPS locations …

Effects Of Early‑Life Experience On Innovation And Problem‑Solving In Captive Coyotes, Andrew C. Garcia, Mitchell A. Parsons, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Early-life experience often shapes behaviors like innovation and exploration. These behaviors are important to animals encountering novel food resources in diverse habitats, such as mesocarnivores in urban areas. To understand if early-life experiences impact later-life behavior, we examined how coyotes (Canis latrans) responded to a multi-access puzzle box at two life stages: pup (~ 7 weeks) and dispersal (~ 10 months). We first exposed pups, still living with their parents and littermates, to a baited puzzle box. At dispersal age, we again tested both these pups and an age-matched control group that was not exposed to the puzzle …

Divergent Neural And Endocrine Responses In Wild-Caught And Laboratory-Bred Rattus Norvegicus, Joanna Jacob, Sally Watanabe, Jonathan Richardson, Nick Gonzales, Emily Ploppert, Garet Lahvis, Aaron Shiels, Sadie Wenger, Kelly Saverino, Janhavi Bhalerao, Brendan Crockett, Erin Burns, Olivia Harding, Krista Fischer-Stenger, Kelly Lambert

United States Department of Agriculture Wildlife Services: Staff Publications

Although rodents have represented the most intensely studied animals in neurobiological investigations for more than a century, few studies have systematically compared neural and endocrine differences between wild rodents in their natural habitats and laboratory strains raised in traditional laboratory environments. In the current study, male and female Rattus norvegicus rats were trapped in an urban setting and compared to weight-and sex-matched conspecifics living in standard laboratory housing conditions. Brains were extracted for neural assessments and fecal boli were collected for endocrine [corticosterone and dehydroepiandrosterone (DHEA)] assays. Additionally, given their role in immune and stress functions, spleen and adrenal weights …

Experimental Infection Of Brazilian Free-Tailed Bats (Tadarida Brasiliensis) With Two Strains Of Sars-Cov-2, Angela M. Bosco-Lauth, Stephanie M. Porter, Karen A. Fox, Mary E. Wood, Daniel Neubaum, Marissa Quilici

United States Department of Agriculture Wildlife Services: Staff Publications

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is presumed to have originated from wildlife and shares homology with other bat coronaviruses. Determining the susceptibility of North American bat species to SARS-CoV-2 is of utmost importance for making decisions regarding wildlife management, public health, and conservation. In this study, Brazilian free-tailed bats (Tadarida brasiliensis) were experimentally infected with two strains of SARS-CoV-2 (parental WA01 and Delta variant), evaluated for clinical disease, sampled for viral shedding and antibody production, and analyzed for pathology. None of the bats (n = 18) developed clinical disease associated with infection, shed infectious virus, or …

Long-Term Effect Of A Gnrh-Based Immunocontraceptive On Feral Cattle In Hong Kong, Rebecca Pinkham, Ka-Kei Koon, Jason To, Jason Chan, Flavie Vial, Matt Gomm, Douglas C. Eckery, Giovanna Massei

United States Department of Agriculture Wildlife Services: Staff Publications

Increasing human-wildlife conflicts worldwide are driving the need for multiple solutions to reducing “problem” wildlife and their impacts. Fertility control is advocated as a non-lethal tool to manage free-living wildlife and in particular to control iconic species. Injectable immunocontraceptives, such as GonaCon, stimulate the immune system to produce antibodies against the gonadotrophin-releasing hormone (GnRH), which in turn affects the release of reproductive hormones in mammals. Feral cattle (Bos indicus or Bos taurus) in Hong Kong are an iconic species whose numbers and impacts on human activities have increased over the last decade. Previous studies have proven that a …