Veterinary Medicine Commons^™

The Effect Of Selenized Yeast Supplementation On Some Performance Parameters In Sheep, Bogumila Pilarczyk, Agnieszka Tomza-Marciniak, Zbigniew Dobrzanski, Malgorzata Szewczuk, Tomasz Stankiewicz, Dariusz Gaczarzewicz, Wlodzimierz Lachowski

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the levels of serum selenium in coarse-haired Pomeranian sheep and to evaluate the effect of the oral supplementation of selenized yeast on selected performance parameters in such sheep. The sheep were divided into 2 groups, with 53 ewes forming the experimental group and 34 ewes forming the control group. The concentration of serum selenium in all 87 sheep from the 2 groups was analyzed. The sheep in the experimental group received selenized yeast for the 3 days before and 3 days after mating (1 g preparation/animal daily, with 1 g of preparation …

Scrapie Cases In The Northern Cyprus, Aydin Gürel, Ahmet Gülçubuk, Nuri̇ Turan, Christopher Richard Helps, Hüseyi̇n Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Scrapie is a fatal neurodegenerative disease affecting small ruminants that causes economic losses in many countries. It is categorized together with other fatal neurodegenerative diseases as a transmissible spongiform encephalopathy. The aim of this study was to investigate clinical cases and the cause of death in a flock of sheep from Northern Cyprus (Turkish Republic of Northern Cyprus) suspected of having scrapie. Brains from 4 sheep showing clinical signs of classical scrapie, out of a flock of 200 animals, were analyzed by histopathological examination and infective prion enzyme-linked immunosorbent assay (ELISA). The 4 affected sheep were 2-3 years of age …

Presence Of Mycoplasmas In The Respiratory System Of Small Ruminants Managed Under An Extensive Production System, Zinka Maksimovic, Christian De La Fe, Maid Rifatbegovic

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the presence of mycoplasmas in the respiratory system of sheep and goat populations managed under extensive production systems. Specific culture and polymerase chain reaction techniques were performed on a total of 88 lung tissue samples and 142 nasal swabs obtained from multiple herds in Bosnia and Herzegovina. A total of 29 samples (12.6%) were found to be positive for mycoplasmas. Mycoplasma ovipneumoniae was detected in the nasal swabs and lungs of affected sheep (n = 19) and in the lungs of asymptomatic goat kids (n = 2), while M. arginini (n = …

Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis

Turkish Journal of Veterinary & Animal Sciences

A 4-year-old Chios ewe was submitted to the Clinic of Farm Animals (School of Veterinary Medicine, Aristotle University of Thessaloniki). The animal had delivered 2 healthy lambs, 2 days earlier, and was inappetent, depressed, and reluctant to move, while it had tachycardia and tachypnea. The cervix was opened at a dilation of 1 finger in diameter and fetid fetal membranes expelled from the vagina. Serum biochemistry revealed renal and liver disease, while ultrasonography revealed uterine dilatation. Laparotomy revealed a partly autolysed embryo in the uterus and gangrenous metritis. Clostridium perfringens and Escherichia coli were isolated in bacterial cultures from the …

Rhodotorula Minuta Fungemia In A Ewe Lamb, C G. Chitko-Mckown, K A. Leymaster, M. P. Heaton, D D. Griffin, J K. Veatch, S A. Jones, M. L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in monocyte cultures as well. Serum from the lamb was submitted to the Veterinary Diagnostic Laboratory at Colorado State University for fungal diagnosis and was found negative for Aspergillus, Blastomyces, Coccidioidomycosis and Histoplasmosis. DNA was isolated from fungi collected from tissue culture supernatants and used in a set of pan-fungal PCR assays with DNA …

The Effect Of Maternal Undernutrition On Muscle Development In The Ovine Fetus*, Berjan Demi̇rtaş, Mukaddes Özcan

Turkish Journal of Veterinary & Animal Sciences

The effect of maternal undernutrition on muscle development of near-term ovine fetuses was studied in 2 experiments. In each experiment, Welsh mountain ewes were randomly assigned to either control (C) or nutrient-restricted (R) groups. In the first experiment, mild maternal undernutrition (85% of daily nutritional requirement (DNR)), and in the second experiment, severe maternal undernutrition (50% of DNR), were imposed between 0 and 70 days of gestation (dg). Controls for both groups were maintained on 100% of their DNR throughout the experiment. In each experiment, ewes were killed at 126 ± 1 dg by an intravenous injection of pentobarbitone. The …

Neuronal And Extraneuronal Lipofuscinosis In Merino Sheep Grazing Asphodelus Aestivus Seeds In Western Turkey, Sümbül Serap Bi̇ri̇nci̇oğlu, Wolfgang Schmahl, Hamdi̇ Avci

Turkish Journal of Veterinary & Animal Sciences

Neuronal and extraneuronal lipofuscinosis was determined in Merino sheep (6 sheep and 3 heads of sheep) of 2-3 years old that had grazed on Asphodelus aestivus seeds. Clinically, the sheep exhibited tremors, paresis, oral and nasal discharge, and severe respiratory distress. Haemorrhagic necrotic tracheitis, aspiration pneumonia, and subacute enteritis were observed in 6 sheep during macroscopic examinations. The brains of the animals were of firm consistency and yellowish brown in colour. Histological examination of the central nervous system revealed that most of the cytoplasm of neurons contained yellowish brown pigment granules. Pigmentations were particularly detected in the pons, medulla oblongata, …

Improvement Studies On Mutton Sheep For Marmara Region Conditions: I. Fertility, Lamb Survival, And Growth Traits Of Lambs, Ayhan Ceyhan, Tamer Sezenler, İsmai̇l Erdoğan, Osman Torun

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to compare the fertility of ewes, the survival rates, and the growth performance of lambs that were crossbred by mating German Black-Headed Mutton (GBM) rams with Kıvırcık ewes. The average lambing rates of the GBM × K(F1), Bandırma-I and Bandırma-II genotype, and Kıvırcık ewes were 80.00%, 75.73%, 76.78%, and 83.72%, respectively. The differences in lambing rates between the genotypes were significant (P < 0.01). The genotypes had a significant effect on litter size at birth, but not on fecundity. The differences in lambs' survival rates between the genotypes were significant (P < 0.05). The birth weight (BW), weaning weight (WW), and yearling live weight (YLW) of the lambs were 3.77 kg, 34.11 kg, and 43.71 kg for the GBM × K (F1); 3.74 kg, 32.98 kg, and 44.91 kg for the Bandırma-I; 3.73 kg, 33.18 kg, and 45.71 kg for the Bandırma-II; and 3.45 kg, 30.92 kg, and 42.22 kg for the Kıvırcık lambs. The results of this study showed that crossbreeding between the GBM and Kıvırcık breed did not significantly increase lamb production. However, crossbred lambs did not have significant survival problems when compared to native Kıvırcık lambs and had higher growth performance than purebred Kıvırcık lambs.

Analysis Of The Orf Region Of The Prion Protein Gene In The Botosani Karakul Sheep Breed From Romania, Steliana Elvira Maria Kevorkian, Mihaela Zaulet, Maria Adina Manea, Sergiu Emil Georgescu, Marieta Costache

Turkish Journal of Veterinary & Animal Sciences

Ovine scrapie is considered a neurodegenerative disease characterized by the accumulation of an abnormal, protease-resistant isoform of PrP. The polymorphisms at codons 136, 154, and 171 are associated with susceptibility to scrapie. In order to detect the existing polymorphisms at the 3 codons we used the real-time PCR technique. The obtained genotypes were confirmed by PCR amplification and sequencing of a 402 bp fragment of exon 3 of the prion gene. Known polymorphisms were detected at codons 136, 154, and 171 and 5 different genotypes were determined. The results showed that all animals were homozygous for A at codon 136 …

Massive Toxoplasma Abortions In A Dairy Sheep Flock And Therapeutic Approach With Different Doses Of Sulfadimidine, Nektarios D. Giadinis, Konstantinos Terpsidis, Anastasia Diakou, Victoria Siarkou, Panayiotis Loukopoulos, Ridvan Osman, Harilaos Karatzias`, Margarita Papazahariadou

Turkish Journal of Veterinary & Animal Sciences

In a 500 head dairy sheep flock in Northern Greece, massive abortions (60%) were observed at 110-130 days of gestation. Attempts were made to treat the abortions with oxytetracyclin L.A. 20% but they were not successful. Toxoplasmosis was confirmed by laboratory examination of blood sera of ewes, aborted fetuses, and fetal brain samples. The remaining 200 pregnant sheep were divided in 2 groups of 100 ewes each. Animals of Group 1 were treated with 20 mg/kg b.w. sulfadimidine every other day 4 times, while the animals in Group 2 received 33 mg/kg b.w. sulfadimidine every other day 4 times via …

Maedi-Visna Virus In Turkish Sheep: A Preliminary Serological Survey Using Elisa Tests, Silvia Preziuso, Mehmet Erman Or, Monica Giammarioli, Abdullah Kayar, Francesco Feliziani, Remzi̇ Gönül, Silvana Farneti, Cağla Parkan Yaramiş, Carlo Valente, Vincenzo Cuteri

Turkish Journal of Veterinary & Animal Sciences

The maedi-visna virus (MVV) is distributed worldwide and, to date, no epidemiological surveys have been carried out using ELISA testing in Turkey. The aims of this study were as follows: i) to verify the diagnostic performance of a home-made ELISA test (HM-ELISA) using Turkish ovine sera in comparison with other commercially available ELISA tests, and ii) to perform a preliminary epidemiological survey in İstanbul province. The MVV seroprevalence in İstanbul province was 15.3%. Older sheep were 5 times more likely to be positive than younger animals, ewes were 3 times more likely to be positive than rams, and all the …

Blood Chimerism Confounds Genetic Relative Susceptibility Testing For Classical Scrapie In Sheep, David A. Schneider, Ahmed Tibary, Terje Taudsepp, Pranab J. Das, Katherine I. O'Rourke

Zoonotics and Wildlife Disease: Publications

Classical scrapie disease is a transmissible spongiform encephalopathy of sheep that is enzootic in the United States. Susceptibility of sheep to classical scrapie is linked to single nucleotide polymorphisms in the prion protein gene (PRNP), forming the basis for genetic testing strategies used by national efforts to eradicate scrapie. Such efforts are occasionally hampered by inconclusive results stemming from the detection of ‘‘complex’’ genotypes. Naturally occurring cases of ovine chimerism are thought to account for some of these instances. In the current report, 4 naturally occurring ovine chimeras are documented through cytogenetic and molecular analyses. All 4 of these sheep …

Nor98 Scrapie Identified In The United States, Christie M. Loiacono, Bruce V. Thomsen, S. Mark Hall, Matti Kiupel, Diane Sutton, Katherine I. O'Rourke, Bradd Barr, Lucy Anthenill, Delwyn Keane

Zoonotics and Wildlife Disease: Publications

A distinct strain of scrapie identified in sheep of Norway in 1998 has since been identified in numerous countries throughout Europe. The disease is known as Nor98 or Nor98-like scrapie, among other names. Distinctions between classic scrapie and Nor98 scrapie are made based on histopathology and immunodiagnostic results. There are also differences in the epidemiology, typical signalment, and likelihood of clinical signs being observed. In addition, sheep that have genotypes associated with resistance to classic scrapie are not spared from Nor98 disease. The various differences between classic and Nor98 scrapie have been consistently reported in the vast majority of cases …

Concentration Of Products Of Nitric Oxide Oxidation And Some Vitamins In Sheep With Naturally Acquired Babesiosis, Handan Mert, İbrahi̇m Yörük, Yeter Değer, Ni̇hat Mert, Semi̇ha Dede, Fatmagül Yur

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to determine serum concentrations of the products of nitric oxide oxidation (nitrate and nitrite) and some vitamins (retinol acetate, \alpha- and \delta-tocopherol, and vitamin D_3) in sheep naturally infected with Babesia ovis. The investigation included 30 infected and 10 control sheep. Serum \alpha -tocopherol levels were significantly lower (P < 0.05), and nitrate and nitrite concentrations were significantly higher (P < 0.05) in infected animals than in controls. It is thought that the elevated nitrate and nitrite levels of the sheep infected with babesiosis were due to the result of damage caused by Babesia. On the other hand, a significant decrease was observed in \alpha -tocopherol levels in sheep with babesiosis because of damage and pathophysiological changes to erythrocytes.

Isolation And Biotyping Of Brucella Melitensis From Aborted Sheep And Goat Fetuses, Esra Büyükcangaz, Ayşi̇n Şen, Serpi̇l Kahya

Turkish Journal of Veterinary & Animal Sciences

The possible role of Brucella spp. in 65 abortion cases, 55 from sheep and 10 from goats, occurring in the birth seasons of 2004 and 2005 in northwestern Turkey was investigated. Colony morphology, agglutination by acriflavin, H2S production, CO2 requirement, dye sensitivity in thionin, basic fuchsin, growth characteristics in streptomycin, lysis with Tbilisi phage, and agglutination with monospecific A- and M antisera were examined for identification and biotyping. The isolates from 21 of 55 sheep and 1 of 10 goat abortion cases were identified as Brucella melitensis. Biotyping revealed that 14, 6, and 1 of 22 Brucella spp. were B. …

Acute Encephalopathy And Clinical Pathology Findings In A Sheep With Chronic Copper Poisoning, Nektarios D. Giadinis, Nikolaos Papaioannoy, Maria Kritsepi-Konstantinou, Nikolaos Roubies, Nikolaos Raikos, Harilaos Karatzias

Turkish Journal of Veterinary & Animal Sciences

A 3-year-old East Friesian sheep from a sheep flock that suffered from chronic copper poisoning was forwarded to the Clinic of Farm Animals in the Aristotle University. The animal had signs of a hemolytic disease indicative of chronic copper poisoning and also nervous signs. Laboratory examinations were consistent with chronic copper poisoning. This was confirmed after necropsy and liver toxicological analysis. By blood smear examination, metarubricytosis, basophilic stippling, anisocytosis, and polychromasia were found. Also, brain histopathological examination revealed focal areas of gliosis in the cerebral cortex and white matter areas. Satellitosis, neuronal degeneration (chromatolysis) and neuronophagia were also observed. These …

Sheep Updates 2008 - Part 2, Meredith L. Sheil, Di Evans, Brown Besier, Tim Scanlon, Andre Martinho De Almeida, Johan Greeff, Tanya Kilminster, John Milton, Chris Oldham, B. L. Mcintyre, Alison Wheatley, John Lucey, Fiona Jones, Nick Costa, Ed Riggall, J. R. L. Hall, Robin Jacob, Dave Pethick, Dave Hopkins, Graham Gardner

Sheep Updates

This session covers eleven papers from different authors: The Sheep Room 1. Analgesia for Surgical Husbandry Procedures in Sheep and Other Livestock, Dr Meredith L. Sheil, Animal Ethics Pty Ltd, Associate Sydney University Faculty of Veterinary Science The Wool Enterprise 2. Unmulsed sheep - implications for chemical use, Di Evans & Brown Besier, Department of Agriculture and Food WA 3. Are Damara and Dorper sheep better adapted than Merinos to nutritional stress? - Growth rates, Tim Scanlon1, Andre Martinho de Almeida2, Johan Greeff1, Tanya Kilminster1, John Milton3, Chris Oldham1, Department of Agriculture and Food WA1, Instituto de Investigacao Cientifica Tropical, …

The Prevalence Of Sheep Liver Trematodes In The Northwest Region Of Iran, Mohammad H. Movassagh Ghazani, Mohammad R. Valilou, Ali R. Ahmadzadeh, Amir R. Karami, Khadijeh Zirak

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the prevalence of liver fluke infection in sheep in the northwest region of Iran (municipal slaughterhouse of Tabriz). The study included the livers of 140 sheep that were collected by systematic random sampling. The prevalence rate of Fasciola hepatica and Dicrocoelium dendriticum was 8.57% and 20%, respectively.

Sheep Updates 2007 - Part 4, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence, David Masters, R. Silberstein, F. Byrne, P. G. H. Nichols, J. Young, L. Aitkins, M. G. Wilmot, A. J. Rintoul, T. Lambert, D. R. Mcclements, P. Raper, P. Ward, C. Walton, T. York, David Pethick, David Hopkins, Malcolm Mcphee, D. B. Savage, J. V. Nolan, I. R. Godwin, A. Aoetpah, T. Nguyen, N. Baillie, C. Lawler, Ken Geenty, John Smith, Darryl Smith, Tim Dyall, Grant Uphill, John Young, Michael Paton, John Dorrian

Sheep Updates

This session covers eight papers from different authors:

GRAZING

1. The impact of high dietary salt and its implications for the management of livestock grazing saline land, Dean Thomas, Dominique Blache, Dean Revell, Hayley Norman, Phil Vercoe, Zoey Durmic, Serina Digby, Di Mayberry, Megan Chadwick, Martin Sillence and David Masters, CRC for Plant-based Management of Dryland Salinity, Faculty of Natural & Agricultural Sciences, The University of Western Australia, WA.

2. Sustainable Grazing on Saline Lands - outcomes from the WA1 research project, H.C. Norman^1,2, D.G. Masters^1,2, R. Silberstein^1,2, F. Byrne^2,3, P.G.H. Nichols …

A Comparison Of Pyronin Y-Methyl Green Stain And Methylene Blue Stain For Somatic Cell Count In Sheep Milk, Emily Mirek, Stacey O’Donnell

Senior Honors Projects

Somatic cell count is a key method used to evaluate the quality of milk in today’s growing dairy sheep industry. Somatic cells are body cells, primarily the white blood cells, found in a milk sample. If an infection such as mastitis is present, the number of somatic cells in the milk increases (Gonzalo, et al. 1992). Producers routinely perform somatic cell counts on cow and goat milk. For dairy cattle in the United States, the somatic cell count can not exceed 750,000 cells per milliliter, for commercial milk. For dairy goats the accepted limit is 1,000,000 cells per mL of …

Development Of An Assay To Determine Single Nucleotide Polymorphisms In The Prion Gene For The Genetic Diagnosis Of Relative Susceptibility To Classical Scrapie In Sheep, Mary Lynn Johnson, Jessica M. Evoniuk, Charles L. Stoltenow, Katherine I. O'Rourke, Dale A. Redmer

Zoonotics and Wildlife Disease: Publications

The objective of this study was to develop a reliable Taqman® 5' Nuclease Assay for genotyping sheep for scrapie susceptibility. The sheep prion gene contains 2 single nucleotide polymorphisms (SNPs) that may mediate resistance to classical scrapie, one at codon 136, alanine (A) or valine (V), and another at codon 171, arginine (R) or glutamine (Q). The R allele appears to confer resistance to classical scrapie, with the AA₁₃₆ RR₁₇₁ genotype the most resistant to scrapie and QR₁₇₁ only rarely infected in the US sheep population. The Assays by Design^SM protocol was used for development of …

Verotoxin Production In Strains Of Escherichia Coli Isolated From Cattle And Sheep, And Their Resistance To Antibiotics, Altan Aksoy, Murat Yildirim, Bi̇rgül Kaçmaz, Teoman Zafer Apan, J. Sedef Göçmen

Turkish Journal of Veterinary & Animal Sciences

In this study, 130 Escherichia coli strains isolated from 306 cattle and sheep fecal samples were studied for their resistance to 10 antibiotics, verotoxin production, and hemolyzation. The antibiotic resistance rates of the E. coli strains were as follows: tetracycline, 51.6%; streptomycin, 24.2%; ampicillin, 13.1%; amoxicillin/clavulanic acid, 5.2%; gentamycin, 4.6%; ciprofloxacin, 4.6%; trimethoprim-sulfamethoxazole, 4.3%; cefotaxime, 0.7%. None of the strains were resistant to cefepime or ceftazidime. Of all the antibiotics tested, only resistance to streptomycin was higher in the strains isolated from cattle than in the sheep strains (P = 0.043). The evaluation of the cattle strains, based on gender …

Biotyping And Serotyping Of Mannheimia (Pasteurella) Haemolytica Isolated From Lung Samples Of Slaughtered Sheep In The Van Region, Zi̇ya İlhan, İhsan Keleş

Turkish Journal of Veterinary & Animal Sciences

A total of 584 lung samples of slaughtered sheep having clinical symptoms of pneumonia were investigated microbiologically. Out of the 584 samples, 66 (11.3%) Mannheimia (Pasteurella) haemolytica strains were isolated on the basis of cultural, morphological and biochemical characteristics. Sixty-six isolates of M. haemolytica were biotyped by carbohydrate fermentation tests and serotyped by coagglutination and indirect haemagglutination (IHA) tests. Out of the 66 M. haemolytica strains, 57 (86.3%) were detected as biotype A, and 9 (13.6%) were biotype T. The 66 M. haemolytica isolates were serotyped as follows by coagglutination test: 13 (19.6%) A2, 11 (16.6%) A1, 9 (13.6%) A6, …

Seroprevalence Of Brucellosis In Human, Sheep, And Cattle Populations In Kırıkkale (Turkey), Teoman Zafer Apan, Murat Yildirim, Ersi̇n İstanbulluoğlu

Turkish Journal of Veterinary & Animal Sciences

The seroprevalence of Brucellosis in human, sheep, and cattle populations was investigated in rural regions of Kırıkkale in Turkey. Serum samples were obtained from 1436 humans, and 3.2% (46) of the population was found to be positive by Rose Bengal Plate test (RBPT) and 3.0% (43) gave positive results with the standard tube agglutination test (STAT). Immunoglobulin class analysis of RBPT-positive sera using the ELISA test gave positive results for 33 people (75%) with Brucella ELISA IgG and for 11 people (25%) with Brucella ELISA IgM. The evaluation of 804 serum samples, which were obtained from 301 cattle and 503 …

Determination Of Clostridium Perfringens Toxin-Types In Sheep With Suspected Enterotoxemia In Kars Province, Turkey, Hali̇l İbrahi̇m Gökce, Oktay Genç, Mahmut Sözmen, Gürbüz Gökçe

Turkish Journal of Veterinary & Animal Sciences

The study was designed to determine the types of Clostridium perfringens and their toxins in sheep with suspected enterotoxemia in Kars province. For this purpose, 260 dead sheep with suspected enterotoxemia and 35 clinically healthy sheep (controls) of different ages and breeds were used. Intestinal contents of each sheep were collected and used to determine alpha, beta, and epsilon toxins of C. perfringens using enzyme-linked immunosorbent assay (ELISA) and the latex agglutination test (LAT). Based on ELISA and LAT, 220 (84.61%) and 152 (58.46%) sheep, respectively, were positive for C. perfringens toxins. In contrast, 40 (15.38%) sheep were toxin-negative based …

Sheep Updates 2006 - Part 1, David Sackett, Kevin Foster, Ronald J. Yates, Phil Nichols, Graeme Martin, John Milton, Scott Williams, Garry Mcalister, Mark Suttie, Peter Fennessy, Jack Cocks

Sheep Updates

This session covers seven papers from different authors:

PLENARY

1. Making Dollars from Merinos, David Sackett, Holmes Sackett & Associates Pty Limited, Wagga Wagga, NSW

2. A new variety of sulla (Hedysarun coronarium)for forage production in southern Australia, Kevin Foster, Ron Yates, Phil Nichols, Department of Agriculture and Food, WA and Centre for Legumes in Mediterranean Agriculture, UWA

3. Mating - Short and fast is better, Graeme Martin, John Milton, Faculty of Natural & Agricultural Sciences, The University of Western Australia

4. Breech strike protection in sheep post 2010, Scott Williams, Program Manager Animal Health and Welfare, Australian Wool …

Investigation Of Serum Cu, Zn, Fe And Se Concentrations During Pregnancy (60, 100 And 150 Days) And After Parturition (45 Days) In Single And Twin Pregnant Sheep, Fuat Gürdoğan, Ati̇lla Yildiz, Engi̇n Balikçi

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the serum Cu, Zn, Se and Fe concentrations at 60, 100, and 150 days of pregnancy and at 45 days of parturition in twin and single pregnant sheep. A total of 30 pregnant Akkaraman sheep in 2 groups (15 single pregnancies, 15 twin pregnancies), aged 3-5 years and weighing 40-50 kg were used. The blood samples were analyzed for serum Cu, Zn, Se and Fe concentrations. No statistically significant difference was found for Se, Cu, Fe and Zn concentrations between the twin and single pregnancy groups. Insignificant decreases (P > 0.05) in serum Zn and …

Changes In Faecal Egg Counts At Different Hours Of The Day And Relationship Between Faecal Egg Count And Parasite Burden In Sheep Naturally Infected With Dicrocoelium Dendriticum, Bayram Şenli̇k, Veli̇ Yilgör Çirak, Mustafa Muz, Recep Tinar

Turkish Journal of Veterinary & Animal Sciences

The present study was carried out to determine the variations in the number of eggs excreted in the faeces at different hours of the day and the correlation between faecal egg counts and fluke burden in sheep naturally infected with Dicrocoelium dendriticum. For this purpose faecal samples were taken from 14 sheep at 1-h intervals from 07^{00} to 19^{00}. Faecal samples were examined by modified Benedek sedimentation method and mean egg counts per gram of faeces (EPG) for each hour (average of group) were calculated. In general, egg counts were found higher in faecal samples taken in the afternoon than …

Application Of Western Blotting And Enzyme Linked Immunosorbent Assay (Elisa) For The Diagnosis Of Dicrocoelium Dendriticum In Sheep Using Excretory Secretory (E/S) Antigens, Sami̇ Şi̇mşek, Ergün Köroğlu, Armağan Erdem Ütük

Turkish Journal of Veterinary & Animal Sciences

Protein bands of excretory/secretory (E/S) antigens of Dicrocoelium dendriticum were determined using SDS-PAGE and Western blotting. The E/S antigens were prepared from live adult D. dendriticum, which were obtained from slaughtered sheep at a local abattoir. In the SDS-PAGE analysis of D. dendriticum E/S antigen, 9 protein bands with molecular weights varying between 6 and 66 kDa with Coomassie staining and 14 bands between 6 and 205 kDa with silver staining were detected. Blood samples were obtained from sheep at the local abattoir. Following the examination of the organs for D. dendriticum and other helminths, serum samples were divided into …

The Importance Of Refractoriness In Ovine Antral Digestive And Interdigestive Motility, Krzysztof Waldemar Romanski

Turkish Journal of Veterinary & Animal Sciences

The aim of this work was to find possible differences between the effects of anticholinergic drug administration during the duodenal phase 1 or phase 2a of migrating myoelectric complex on antral spike burst amplitude in fasted and non-fasted sheep. Eight adult rams possessing bipolar electrodes in the antrum and small intestine were used in the study. Feeding increased significantly the spike burst amplitude range from 60-110 to 80-160 µV. The effects of hexamethonium (2 mg/kg), atropine (0.1 mg/kg) and pirenzepine (0.5 mg/kg) given i.v. during phase 1 of the migrating myoelectric complex (expressed as per cent of control) in non-fasted …