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Articles 31 - 60 of 253

Full-Text Articles in Veterinary Medicine

Comparative Evaluations Of Tear Secretion In Healthy And Infectious Keratoconjunctivitis Romanov Sheep With Schirmer Tear Test And Phenol Red Thread Tear Test, Mustafa Bariş Akgül, Ni̇hat Şindak, Ali̇ Gülaydin, Doğukan Özen, Serpi̇l Kahya Demi̇rbi̇lek, Kadi̇r Sulu Jan 2017

Comparative Evaluations Of Tear Secretion In Healthy And Infectious Keratoconjunctivitis Romanov Sheep With Schirmer Tear Test And Phenol Red Thread Tear Test, Mustafa Bariş Akgül, Ni̇hat Şindak, Ali̇ Gülaydin, Doğukan Özen, Serpi̇l Kahya Demi̇rbi̇lek, Kadi̇r Sulu

Turkish Journal of Veterinary & Animal Sciences

The aim of this study is to determine the amount of tear secretion in sheep with infectious keratoconjunctivitis and healthy ones in the Siirt region by Schirmer tear test and phenol red thread tear test. A total of 47 Romanov sheep, consisting of sheep with infectious keratoconjunctivitis (n = 6) and healthy sheep (n = 41), constituted the study material. Phenol red thread tear test followed by Schirmer tear test was performed on both eyes of the animals, properly taken at a control visit on their feet. The Schirmer tear test and phenol red thread tear test values were measured …


Molecular Identification Of Tick-Borne Pathogens In Tickhaemaphysalis Longicornis From Sheep In Henan, China, Yongshuai Peng, Meng Qi, Fuchun Jian, Jinhong Wang, Yali Lv, Jiujian Wei, Rongjun Wang, Longxian Zhang, Changshen Ning Jan 2017

Molecular Identification Of Tick-Borne Pathogens In Tickhaemaphysalis Longicornis From Sheep In Henan, China, Yongshuai Peng, Meng Qi, Fuchun Jian, Jinhong Wang, Yali Lv, Jiujian Wei, Rongjun Wang, Longxian Zhang, Changshen Ning

Turkish Journal of Veterinary & Animal Sciences

Tick-borne diseases are one of the most important classes of disease in animal husbandry and cause severe economic losses. In this study, 660 adult female ticks were collected from sheep in nine localities in Henan Province, China. All were identified as Haemaphysalis longicornis, confirmed with light microscopy and PCR amplification. The pathogens identified in these ticks included bacteria of the genus Anaplasma (Anaplasma ovis, A. bovis, A. phagocytophilum) and piroplasmal protozoans (Theileria luwenshuni, Babesia motasi). Our results show the high prevalence of A. bovis (20.4%, 135/660) and T. luwenshuni (14.4%, 95/660) in the ticks from sheep, whereas A. ovis, A. …


Bovine And Ovine Schistosomiasis: Prevalence And Associated Host Factors In Selected Sites Of South Achefer District, Northwest Ethiopia, Yirsaw Kerie, Zewdu Seyoum Dec 2016

Bovine And Ovine Schistosomiasis: Prevalence And Associated Host Factors In Selected Sites Of South Achefer District, Northwest Ethiopia, Yirsaw Kerie, Zewdu Seyoum

The Thai Journal of Veterinary Medicine

Schistosomiasis is a snail-borne trematode infection in man and animals in tropical and subtropical countries. It is an economically important disease caused by several Schistosoma species and results in economic losses through mortality and morbidity from severe infection and from long-term effect of moderate- and long-standing chronic infection. This study was conducted from November 2013 to April 2014 to determine the prevalence and potential host-related risk factors of bovine and ovine schistosomiasis in selected sites of South Achefer district, northwest Ethiopia. About 532 faecal samples were collected from randomly selected cattle and sheep from three purposively selected peasant associations. The …


Comparison Of Pcr Tests For The Detection Of Mycoplasma Agalactiae In Sheep And Goats, Hüban Göçmen, Ruben Sebastian Rosales, Roger David Ayling, Mi̇hri̇ban Ülgen Jan 2016

Comparison Of Pcr Tests For The Detection Of Mycoplasma Agalactiae In Sheep And Goats, Hüban Göçmen, Ruben Sebastian Rosales, Roger David Ayling, Mi̇hri̇ban Ülgen

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare PCR tests for the detection of Mycoplasma agalactiae in sheep and goats. Samples obtained from sheep and goats in Turkey were tested to determine the presence of M. agalactiae. Mycoplasma culture and two direct PCR methods based on the uvrC gene and polC gene were compared with PCR of the 16S rRNA gene followed by denaturing gradient gel electrophoresis (PCR/DGGE) on DNA extracted from clinical samples (following culture enrichment). A total of 234 samples were examined: 9.4% and 13.2% were positive for M. agalactiae based on the uvrC gene and polC gene …


Cast/Mspi Gene Polymorphism And Its Impact On Growth Traits Of Soviet Merinoand Salsk Sheep Breeds In The South European Part Of Russia, Ivan Fedorovich Gorlov, Nadezhda Vasilevna Shirokova, Alexander Vasilevich Randelin, Valeriya Nikolaevna Voronkova, Natalya Ivanovna Mosolova, Elena Yurevna Zlobina, Yury Anatolevich Kolosov, Nekruz Farkhodovich Bakoev, Mariya Anatolevna Leonova, Sirozhdin Yusufovich Bakoev, Anatoly Yurevich Kolosov, Lubov Vladimirovna Getmantseva Jan 2016

Cast/Mspi Gene Polymorphism And Its Impact On Growth Traits Of Soviet Merinoand Salsk Sheep Breeds In The South European Part Of Russia, Ivan Fedorovich Gorlov, Nadezhda Vasilevna Shirokova, Alexander Vasilevich Randelin, Valeriya Nikolaevna Voronkova, Natalya Ivanovna Mosolova, Elena Yurevna Zlobina, Yury Anatolevich Kolosov, Nekruz Farkhodovich Bakoev, Mariya Anatolevna Leonova, Sirozhdin Yusufovich Bakoev, Anatoly Yurevich Kolosov, Lubov Vladimirovna Getmantseva

Turkish Journal of Veterinary & Animal Sciences

The purpose of this work was to study MspI polymorphism in the CAST gene in Soviet Merino and Salsk sheep breeds in the South European part of Russia and to find its relation with growth traits. Soviet Merino sheep have been found to have the M and N alleles with a frequency of 0.88 and 0.12 and the MM, MN, and NN genotypes with a frequency of 0.82, 0.12, and 0.06, respectively. Salsk sheep have been established to have the M and N alleles with a frequency of 0.89 and 0.11 and two genotypes, MM and MN, with a frequency …


Ovine Coccidiosis: Prevalence And Associated Risk Factors Inand Around Addis-Zemen, Northwest Ethiopia, Abinet Lakew, Zewdu Seyoum Jan 2016

Ovine Coccidiosis: Prevalence And Associated Risk Factors Inand Around Addis-Zemen, Northwest Ethiopia, Abinet Lakew, Zewdu Seyoum

Turkish Journal of Veterinary & Animal Sciences

This cross-sectional study was conducted in and around Addis-Zemen from November 2014 to April 2015 to determine the prevalence of ovine coccidiosis and to identify associated risk factors. Faecal samples were collected directly from the rectum of 384 randomly selected animals. A centrifugal faecal flotation technique using saturated salt solution was used to detect Eimeria oocysts. Out of 384 samples, only 88 were found to contain Eimeria oocysts. Eimeria infection was recorded in all age and sex groups of the study animals. A strong significant association was observed (P < 0.05) between Eimeria infection and the faecal consistency, production system, hygienic status, age, and body condition of the sampled animals. However, an insignificant (P > 0.05) association was recorded between Eimeria infection and the origins and …


Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş Jan 2016

Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş

Turkish Journal of Veterinary & Animal Sciences

Phospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot®BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic …


Seroprevalence Of Caseous Lymphadenitis In Kangal Akkaraman Sheep, Öznur Aslan, Kadi̇r Semi̇h Gümüşsoy, İlknur Karaca Bekdi̇k, Aytaç Akçay, Ömer Orkun Demi̇ral Jan 2016

Seroprevalence Of Caseous Lymphadenitis In Kangal Akkaraman Sheep, Öznur Aslan, Kadi̇r Semi̇h Gümüşsoy, İlknur Karaca Bekdi̇k, Aytaç Akçay, Ömer Orkun Demi̇ral

Turkish Journal of Veterinary & Animal Sciences

In this study, the seroprevalence of Corynebacterium pseudotuberculosis, the causative agent of caseous lymphadenitis (CLA) infection, in Kangal Akkaraman sheep flocks was examined. For this purpose, blood samples were collected from 25 different sheep flocks (n = 379). According to the ELISA results, the numbers of CLA-positive and CLA-negative samples were 138 (36.4%) and 241 (63.6%), respectively. CLA positivity was observed in 35.7% of the females (n = 241), 37.7% of the males (n = 138), 40.2% of the sheep with clinical symptoms (n = 184), and 32.8% of the sheep with no clinical symptoms (n = 195) (P > 0.05). …


Evaluation Of Tear And Serum Trace Elements (Copper, Selenium, And Cobalt) In Sheep, Duygu Tarhan, Si̇nem Ülgen, Fatma Ateş Alkan, Di̇lek Olgun Erdi̇kmen, Çağla Parkan Yaramiş, Mehmet Erman Or, Ümi̇t Bora Barutçu Jan 2016

Evaluation Of Tear And Serum Trace Elements (Copper, Selenium, And Cobalt) In Sheep, Duygu Tarhan, Si̇nem Ülgen, Fatma Ateş Alkan, Di̇lek Olgun Erdi̇kmen, Çağla Parkan Yaramiş, Mehmet Erman Or, Ümi̇t Bora Barutçu

Turkish Journal of Veterinary & Animal Sciences

Metabolic diseases such as enzootic ataxia, white muscle disease, and white liver disease are among the most important problems of livestock for sheep. The aim of this study was to analyze whether tears can be used as an alternative diagnostic method in determination of diseases that result from lack or excess of copper, selenium, and cobalt. Samples of serum and tears taken from 45 healthy sheep of the Kivircik breed aged between 2 and 4 years were analyzed. The concentrations of copper, selenium, and cobalt were measured in serum and tears as 118.0 ± 36.0, 58.0 ± 15.0, and 0.5 …


Polymorphism Of Exon 2 Of Dio2 Gene And Its Association Withseasonal Reproduction In Sheep, Jianning He, Dongwei Huang, Ran Di, Jinxin Wang, Mingxing Chu, Qiuyue Liu, Wenping Hu, Xiangyu Wang, Zhangyuan Pan Jan 2016

Polymorphism Of Exon 2 Of Dio2 Gene And Its Association Withseasonal Reproduction In Sheep, Jianning He, Dongwei Huang, Ran Di, Jinxin Wang, Mingxing Chu, Qiuyue Liu, Wenping Hu, Xiangyu Wang, Zhangyuan Pan

Turkish Journal of Veterinary & Animal Sciences

DIO2 was studied as a candidate gene for reproduction in sheep. Three pairs of primers were designed to detect single nucleotide polymorphisms (SNPs) of exon 2 of DIO2 in nonseasonal and seasonal reproduction breeds. The polymorphisms were detected using SeqMan II and analyzed by PCR-RFLP. The sheep DIO2 gene was cloned and its mutations were detected in five sheep breeds whose reproductive seasonality and litter sizes were different. Association analyses were performed to reveal the relationships between SNPs and reproductive seasonality as well as litter size in sheep. The results showed that a 1041-bp DNA fragment of the sheep DIO2 …


Presence Of Carbohydrate-Digesting Enzymes Throughout The Digestive Tract Of Sheep, Renata Miltko, Grzegorz Belzecki, Barbara Kowalik, Jacek Skomial Jan 2016

Presence Of Carbohydrate-Digesting Enzymes Throughout The Digestive Tract Of Sheep, Renata Miltko, Grzegorz Belzecki, Barbara Kowalik, Jacek Skomial

Turkish Journal of Veterinary & Animal Sciences

The activity of enzymes that digest carbohydrates at various points in the gastrointestinal tract of sheep was examined. In addition, weight and contents of digestive tract segments were examined. The experiment was performed on six adult ewes fed an 85% hay diet. The morphometric measurements revealed that the alimentary tract constituted 32% of the sheep's body mass and wet rumen contents constituted 70% of wet mass of the total digestive tract content. The protozoal population in the rumen varied from 37.2 × 104 to 54.0 × 104/mL rumen fluid. In the examined samples, ciliates were identified as belonging to the …


Gastrointestinal Nematodes Of Ovis Aries In Eastern Cape, South Africa And An Evaluation Of Current Anthelmintic Procedures, Melyssa Rowell Jan 2015

Gastrointestinal Nematodes Of Ovis Aries In Eastern Cape, South Africa And An Evaluation Of Current Anthelmintic Procedures, Melyssa Rowell

Honors Theses and Capstones

Humans have known about helminth infections since ancient times. Today half of the human population is plagued by a nematode infection. Nematodes are responsible for billions of dollars in global crop damage annually and have had devastating effects on the global livestock industry. Due to the lack of a vaccine, and only a handful of approved classes of anthelmintics, nematode resistance has become a serious global phenomenon, and the South African commercial sheep industry has been one of the worst affected worldwide. The current study used fecal float parasitology to determine the nematode species found on a commercial sheep farm …


The Genetic Diversity Of Both Fecb Gene And Microsatellite Gc101is Associated With Reproduction Selection In Sheep, Zhangyuan Pan, Baoyun Zhang, Wenping Hu, Ran Di, Qiuyue Liu, Xiangyu Wang, Dehua Yin, Pingqing Wang, Mingxing Chu Jan 2015

The Genetic Diversity Of Both Fecb Gene And Microsatellite Gc101is Associated With Reproduction Selection In Sheep, Zhangyuan Pan, Baoyun Zhang, Wenping Hu, Ran Di, Qiuyue Liu, Xiangyu Wang, Dehua Yin, Pingqing Wang, Mingxing Chu

Turkish Journal of Veterinary & Animal Sciences

The aims of study were to reveal the changes in genetic diversity in reproduction selection of sheep. Genetic polymorphisms of microsatellite GC101 locus and its linkage with the FecB gene were detected in Chinese local sheep (Small Tail Han and Hu sheep) and western sheep (Texel and Dorset) breeds. There was the same FecB mutation (A746G) in both Small Tail Han and Hu sheep as that in Booroola Merino ewes, but not in Texel and Dorset sheep. Seven alleles, from 192 bp to 210 bp, and 20 genotypes were detected at the GC101 locus in 447 individuals totally. The preponderant …


Prevalence Of Toxoplasma Gondii In Sheep Meats Purchased From Retailstores In Central Anatolia, Turkey, Kader Yildiz, Fatma Çi̇ğdem Pi̇şki̇n, Armağan Erdem Ütük, Sami̇ Gökpinar Jan 2015

Prevalence Of Toxoplasma Gondii In Sheep Meats Purchased From Retailstores In Central Anatolia, Turkey, Kader Yildiz, Fatma Çi̇ğdem Pi̇şki̇n, Armağan Erdem Ütük, Sami̇ Gökpinar

Turkish Journal of Veterinary & Animal Sciences

Toxoplasmosis is one of the most important foodborne parasitic diseases of humans. In particular, sheep muscles are significant sources of infection in the transmission of toxoplasmosis. Carnivorism is the most important transmission route for human populations. The aim of this study was to detect the prevalence of Toxoplasma gondii tissue cysts in sheep meats in retail stories of Turkey. A total of 250 boneless sheep meat samples were purchased from randomly selected retail stores in different locations of Ankara and Kırıkkale provinces of Turkey. The homogenized meat samples were centrifuged with Percoll dilutions. The tissue cysts were removed by pipette …


Impact Of B. Melitensis Rev-1 Vaccination On Brucellosis Prevalence, Adosinda Maria Coelho, Maria De Lurdes Pinto, Juan Garcia Diez, Ana Claudia Coelho Jan 2015

Impact Of B. Melitensis Rev-1 Vaccination On Brucellosis Prevalence, Adosinda Maria Coelho, Maria De Lurdes Pinto, Juan Garcia Diez, Ana Claudia Coelho

Turkish Journal of Veterinary & Animal Sciences

Trás-os-Montes e Alto Douro, in the northeastern region of Portugal, previously exhibited the highest brucellosis prevalence in the entire small ruminant population of Portugal. Consequently, a vaccination program of the whole population with Brucella melitensis Rev-1 was carried out from 2001 to 2004, and further compulsory Rev-1 vaccination of 3- to 6-month-old lambs and kids was carried out between 2005 and 2007. The prevalence of brucellosis decreased by 71.42% in 2004, with a 2-fold decrease occurring from 2005 to 2007. The reduction of brucellosis prevalence was statistically significant (P < 0.001) according to flock size. By species, brucellosis prevalence decreased 14.5-fold in sheep and 5.5-fold in goats in 2004. Regarding flock constitution, it decreased by 77% for pure flocks and 73% in mixed flocks in 2004. Regarding the animals involved in production, brucellosis prevalence decreased during the 7-year period. It was concluded that Rev-1 vaccination of the whole population was effective in decreasing brucellosis prevalence. These results contribute to the scarce information available regarding the effect of Rev-1 vaccination on different characteristics of flocks. They may be used to improve the efficiency of brucellosis eradication programs within livestock management.


Histomorphometric And Fractal Analysis Of Femoral, Tibial, And Metatarsal Compact Bone Samples In Sheep (Ovis Aries), Goat (Capra Hircus), And Roe Deer (Capreolus Capreolus), Alexandru Gudea, Andrei Stefan Jan 2015

Histomorphometric And Fractal Analysis Of Femoral, Tibial, And Metatarsal Compact Bone Samples In Sheep (Ovis Aries), Goat (Capra Hircus), And Roe Deer (Capreolus Capreolus), Alexandru Gudea, Andrei Stefan

Turkish Journal of Veterinary & Animal Sciences

Histological sections of femur, tibia, and metatarsals originating from sheep (Ovis aries), goat (Capra hircus), and roe deer (Capreolus capreolus) were examined in this study. Our research focused mainly on the quantitative characteristics of the microstructure of these bones. The areas, perimeters, minimum and maximum diameters of the secondary Haversian units, as well as structures of the primary osteonal units were assessed using comparative statistical methods. In addition to these parameters, the present paper investigated the usefulness of fractal analysis (fractal dimension and lacunarity), a new approach that quantifies the complexity and the emptiness pattern of biological structures. Moreover, our …


Polymorphisms Of Ovine Prion Protein (Prp) Gene In Pramenka Sheepbreed Population In Bosnia And Herzegovina, Ervin Zecevic, Admir Dokso, Amra Kazic, Muhamed Brka Jan 2015

Polymorphisms Of Ovine Prion Protein (Prp) Gene In Pramenka Sheepbreed Population In Bosnia And Herzegovina, Ervin Zecevic, Admir Dokso, Amra Kazic, Muhamed Brka

Turkish Journal of Veterinary & Animal Sciences

Transmissible spongiform encephalopathies (TSEs) are a group of diseases that affect the nervous system and lead to death. The putative infectious agent is the host-encoded prion protein (PrP) and it appears that the development of scrapie in sheep is closely related to polymorphisms in the host PrP gene. The aim of this study was to investigate three well-known polymorphisms in exon 3 of the PrP gene (at codons 136, 154, and 171) that have a clear and significant effect on scrapie in sheep. Four other polymorphisms (at codons 145, 185, 231, and 237) that are yet undefined were also investigated. …


Genetic Relationships Among Four Turkish Sheep Breeds Using Microsatellites, Onur Yilmaz, Tamer Sezenler, Semi̇h Sevi̇m, İbrahi̇m Cemal, Orhan Karaca, Yalçin Yaman, Orhan Karadağ Jan 2015

Genetic Relationships Among Four Turkish Sheep Breeds Using Microsatellites, Onur Yilmaz, Tamer Sezenler, Semi̇h Sevi̇m, İbrahi̇m Cemal, Orhan Karaca, Yalçin Yaman, Orhan Karadağ

Turkish Journal of Veterinary & Animal Sciences

The genetic structure of 4 Turkish breeds of sheep, Gökçeada, Kıvırcık, Karacabey Merino, and Sakız (SZ), was analyzed by genotyping with 17 microsatellite markers in 250 individuals. The microsatellites showed high levels of polymorphism, with means for total and effective number of alleles per locus of 20.71 and 7.04, respectively. The average observed and expected heterozygosity values were 0.66 and 0.87, respectively. It was found that a higher level of breed admixture existed in the 4 breeds, which was an important finding. It was also found that 7.9% of genetic variation resulted from difference between the populations, whereas 92.1% resulted …


Determination Of Serum Cardiac Biomarkers And Plasma D-Dimer Levelsin Anemic Sheep With Babesiosis, Özlem Orunç Kilinç, Yaşar Göz, Nazmi̇ Yüksek, Yildiray Başbuğan, Ali̇ Bi̇lgi̇n Yilmaz, Ahmet Duran Ataş Jan 2015

Determination Of Serum Cardiac Biomarkers And Plasma D-Dimer Levelsin Anemic Sheep With Babesiosis, Özlem Orunç Kilinç, Yaşar Göz, Nazmi̇ Yüksek, Yildiray Başbuğan, Ali̇ Bi̇lgi̇n Yilmaz, Ahmet Duran Ataş

Turkish Journal of Veterinary & Animal Sciences

In this study, the cardiac effects of anemia and venous thromboembolism (VTE), reportedly caused by the hemolyzed erythrocytes occurring in sheep with babesiosis, were investigated using cardiac markers and D-dimer (DD). The study included 34 sheep: 24 infected Akkaraman sheep (1-3 years old, diagnosed with babesiosis based on clinical and laboratory findings) and a control group of 10 noninfected healthy sheep of the same breed and age. Hematocrit (Hct) levels were measured in blood obtained from both groups. Cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and aspartate aminotransferase (AST) levels were measured in serum samples. In addition, the levels of …


Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani Mar 2014

Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani

The Thai Journal of Veterinary Medicine

Leptospira is an emerging global public health problem which involves both animals and human. The aim of this study was to appraise the presence of Leptospira DNA in blood samples from cattle and sheep via Polymerase Chain Reaction (PCR) method in Chaharmahal VaBakhtiari and Isfahan provinces of Iran. One hundred and ninety five samples (92 blood samples of cattle and 103 blood samples of sheep) were collected randomly from clinical healthy animals. DNA was extracted from the blood samples and stored at -20°C before examination. PCR reaction was performed for detection of Leptospira DNA using specific primers for 16s rRNA …


The Relationship Between Seropositivity And Tissue Cysts In Sheep Naturally Infected With Toxoplasma Gondii, Kader Yildiz, Oğuz Kul, Sami̇ Gökpinar, Hasan Tarik Atmaca, Yilmaz Emre Gençay, Aycan Nuri̇ye Gazyağci, Cahi̇t Babür, İsmayi̇l Safa Gürcan Jan 2014

The Relationship Between Seropositivity And Tissue Cysts In Sheep Naturally Infected With Toxoplasma Gondii, Kader Yildiz, Oğuz Kul, Sami̇ Gökpinar, Hasan Tarik Atmaca, Yilmaz Emre Gençay, Aycan Nuri̇ye Gazyağci, Cahi̇t Babür, İsmayi̇l Safa Gürcan

Turkish Journal of Veterinary & Animal Sciences

Skeletal muscles (tongue, masseter, leg, intercostal, and diaphragmatic muscles) and brain samples of 100 sheep at slaughter were analysed for the presence of T. gondii tissue cysts along with serum IgG titres. Two methods of isolation by percoll gradient centrifugation and tissue microarray (TMA) technique with immunoperoxidase staining were used. Seropositivity was detected in 88% (88/100) of sheep sera analysed by indirect fluorescent antibody test. Tissue cysts were observed in 46 (52.3%, 46/88) and 15 (17%, 15/88) of the seropositive sheep with the isolation technique and TMA and immunoperoxidase staining, respectively. The diameters of the tissue cysts were 25–58 × …


Repeated Superovulation Treatments In Kivircik Ewes During Breeding And Nonbreeding Seasons, Burcu Üstüner, Seli̇m Alçay, Yavuz Nak, Zekari̇ya Nur, Deni̇z Nak, Bi̇lgi̇ner Tuna, Gözde Şi̇mşek, Hakan Sağirkaya Jan 2014

Repeated Superovulation Treatments In Kivircik Ewes During Breeding And Nonbreeding Seasons, Burcu Üstüner, Seli̇m Alçay, Yavuz Nak, Zekari̇ya Nur, Deni̇z Nak, Bi̇lgi̇ner Tuna, Gözde Şi̇mşek, Hakan Sağirkaya

Turkish Journal of Veterinary & Animal Sciences

The present study compared superovulation and embryo recovery rates applied repeatedly during breeding and nonbreeding seasons in the same 20 Kivircik sheep. Trials 1 and 3 were performed during the breeding season and the trial 2 was performed in the nonbreeding season. We synchronized the sheep's estrus cycles and induced a superovulatory response with porcine follicle stimulating hormone (pFSH) injections. Natural mating took place 12 h after the last FSH injection. The embryos were collected by laparotomy 7 days after natural mating. There was no significant difference between the number of corpora lutea (CL) counted following the 1st (breeding) and …


The Effects Of Supplementing Diets Fed To Pregnant And Lactating Ewes With Saccharomyces Cerevisiae Dried Yeast, Katarzyna Zabek, Stanislaw Milewski, Roman Wójcik, Andrzej Krzysztof Siwicki Jan 2014

The Effects Of Supplementing Diets Fed To Pregnant And Lactating Ewes With Saccharomyces Cerevisiae Dried Yeast, Katarzyna Zabek, Stanislaw Milewski, Roman Wójcik, Andrzej Krzysztof Siwicki

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to determine the effect of supplementing diets fed to ewes (in late pregnancy and during lactation or only during lactation) with Saccharomyces cerevisiae dried brewer’s yeast on the yield and chemical composition of milk and on selected parameters of humoral and cellular immunity. The investigated indicators were analyzed on days 28 and 70 of lactation. The addition of dried yeast contributed to a significant increase in milk yield, and the obtained milk was characterized by a lower somatic cell count, suggesting improved mammary gland health. Diet supplementation had a beneficial influence on the health …


Comparison Of Immune Responses During Gastrointestinal Helminth Self-Cure Expulsion Between Resistant Gulf Coast Native And Susceptible Suffolk Sheep, Javier Jesus Garza Jan 2014

Comparison Of Immune Responses During Gastrointestinal Helminth Self-Cure Expulsion Between Resistant Gulf Coast Native And Susceptible Suffolk Sheep, Javier Jesus Garza

LSU Doctoral Dissertations

The immune response to the self-cure phenomenon seen during gastrointestinal nematode (GIN) parasitism of small ruminants was compared between sheep breeds that are resistant or susceptible to Haemonchus contortus infection. Fifty-four Gulf Coast Native (resistant) and Suffolk (susceptible) lambs were allowed to acquire a natural GIN infection on pasture and were then randomly allocated into 4 groups. After being moved to parasite free housing for 2 months, lambs were given a challenge infection of 20,000 H. contortus L3. Fecal egg counts (FEC) were monitored throughout the study and animals were necropsied at 0, 1, 3, and 7 days post infection …


Evaluation Of Ultrasonography As A Diagnostic Tool For Hepatic Hydatid Cysts In Sheep, Hussein Awad Hussein, Mohammed Elrashidy Jan 2014

Evaluation Of Ultrasonography As A Diagnostic Tool For Hepatic Hydatid Cysts In Sheep, Hussein Awad Hussein, Mohammed Elrashidy

Turkish Journal of Veterinary & Animal Sciences

This retrospective study was designed to evaluate the use of ultrasonography for the diagnosis of hepatic hydatid cysts. This study was conducted on 22 sheep, which were classified into 2 groups according to their liver health group (healthy liver = 13 and cystic liver = 9). Biochemically, serum concentrations of gamma-glutamyl transferase, aspartate aminotransferase, total bilirubin, and globulins were significantly increased (P < 0.01) while albumin was lowered (P < 0.01) in sheep with cystic livers. Ultrasonographically, rounded, anechoic, and unilocular hydatid cysts with elliptical circumferences ranging from 6 to 10 cm were seen. The interiors of cysts contained echogenic particulate materials, septations, or fine echoes. At the 10th intercostal space (ICS), ventral margin, size, thickness, and angle of livers increased in sheep with hepatic cysts, while the diameter of portal vein decreased in sheep with liver cysts. At the 9th ICS, the circumference of the gall bladder was lowered in sheep with hepatic cysts (P < 0.01). The sensitivity, specificity, and positive and negative predictive values of ultrasonography for diagnosis of hepatic hydatid cysts were 80%, 100%, and 100% and 83%, respectively. In conclusion, ultrasonography alone or in combination with some biochemical parameters reflecting liver function could be helpful for the diagnosis of hepatic hydatid cysts in sheep.


New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin Jan 2014

New Understanding Of An Old Phenomenon: Uncontrolled Factors And Misconceptions That Cast A Shadow Over Studies Of The ‘Male Effect’ On Reproduction In Small Ruminants, Trina Jorre De St Jorre, Penny Alison Rhian Hawken, Graeme Bruce Martin

Turkish Journal of Veterinary & Animal Sciences

In female sheep and goats, exposure to males can be used to induce ovulation and thus control and synchronise fertility. This 'male effect' offers simple, cost-effective, and hormone-free management of reproduction in flocks and herds but, despite decades of research, significant gaps in our knowledge impede commercial application. Many of these gaps can be traced back to misconceptions in the early documentation of the male effect, and others are the result of uncontrolled factors in experimental design. Consequently, it is often difficult to draw conclusions from past research, to further our understanding of the phenomenon, and to make recommendations for …


The Influence Of Pomegranate-Peel Extracts On In Vitro Gas Production Kinetics Of Rumen Inoculum Of Sheep, Mohammad Javad Abarghuei, Yousef Rouzbehan, Abdel-Fattah Salem Jan 2014

The Influence Of Pomegranate-Peel Extracts On In Vitro Gas Production Kinetics Of Rumen Inoculum Of Sheep, Mohammad Javad Abarghuei, Yousef Rouzbehan, Abdel-Fattah Salem

Turkish Journal of Veterinary & Animal Sciences

Different levels of pomegranate peel extracted by either water (PEH) or solvent mixture (PEM) were evaluated for their effect on in vitro gas production and ruminal fermentation patterns. Two levels of PEH or PEM (15 and 30 mg total phenolics (TP)/g DM of basal diet) were compared to a control (0) level. Ruminal fermentation parameters such as 24-h partitioning factor (PF_{24}), gas yield (GY_{24}), in vitro organic matter disappearance (IVOMD), metabolizable energy (ME), and microbial protein (MP) were calculated. The addition of PEH or PEM had no effect (P > 0.05) on asymptotic gas production, apparent degraded substrate (ADS), IVOMD, ME, …


Polymorphism Of The Ovine Calpastatin Gene In Some Turkish Sheep Breeds, Onur Yilmaz, Tamer Sezenler, Nezi̇h Ata, Yalçin Yaman, İbrahi̇m Cemal, Orhan Karaca Jan 2014

Polymorphism Of The Ovine Calpastatin Gene In Some Turkish Sheep Breeds, Onur Yilmaz, Tamer Sezenler, Nezi̇h Ata, Yalçin Yaman, İbrahi̇m Cemal, Orhan Karaca

Turkish Journal of Veterinary & Animal Sciences

The calpastatin (CAST) gene has a major effect on muscle growth and meat tenderness after slaughter; it is located on the fifth chromosome in sheep. Blood samples were collected from 720 animals in total from Kıvırcık (KIV), Sakız (SZ), Karacabey Merino (KM), and Gökçeada (GA) sheep populations raised in West Anatolia. The PCR products were digested by the restriction endonuclease MspI. Allele frequencies for M and N alleles of the gene were found to be 0.85 and 0.15 in KIV, 0.80 and 0.20 in KM, 0.99 and 0.01 in GA, and 0.34 and 0.66 in SZ sheep, respectively. It was …


Rhodotorula Minuta Fungemia In A Ewe Lamb, C. G. Chitko-Mckown, K. A. Leymaster, M. P. Heaton, D. D. Griffin, J. K. Veatch, S. A. Jones, M. L. Clawson Jan 2013

Rhodotorula Minuta Fungemia In A Ewe Lamb, C. G. Chitko-Mckown, K. A. Leymaster, M. P. Heaton, D. D. Griffin, J. K. Veatch, S. A. Jones, M. L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in monocyte cultures as well. Serum from the lamb was submitted to the Veterinary Diagnostic Laboratory at Colorado State University for fungal diagnosis and was found negative for Aspergillus, Blastomyces, Coccidioidomycosis and Histoplasmosis. DNA was isolated from fungi collected from tissue culture supernatants and used in a set of pan-fungal PCR assays with …


Effect Of Different Transport Temperatures On In Vitro Maturation Of Oocytes Collected From Frozen-Thawed Sheep Ovaries, Özen Banu Özdaş, Alper Baran, Muzaffer Taş, Ümüt Ci̇ri̇t, Kamber Demi̇r, Süleyman Bacinoğlu, Serhat Pabuccuoğlu, Kemal Ak Jan 2013

Effect Of Different Transport Temperatures On In Vitro Maturation Of Oocytes Collected From Frozen-Thawed Sheep Ovaries, Özen Banu Özdaş, Alper Baran, Muzaffer Taş, Ümüt Ci̇ri̇t, Kamber Demi̇r, Süleyman Bacinoğlu, Serhat Pabuccuoğlu, Kemal Ak

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of 2 different transport temperatures on the in vitro maturation of oocytes collected from frozen-thawed sheep ovaries. Sheep ovaries were transferred into saline at temperatures of 4 °C and 32 °C. After the 2 experimental groups (A: fresh cortex, B: frozen-thawed cortex) were formed, each group was divided into 2 subgroups (group A1: 4 °C, group A2: 32 °C [control]; group B1: 4 °C, group B2: 32 °C). The cortexes were dissected into slices 1-3 mm thick and pieces of 0.5 cm2. For groups B1 and B2, 1-2 cortex pieces …