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Full-Text Articles in Veterinary Medicine

Chronic Toxicity Study Of Garcinia Mangostana Linn. Pericarp Extract, Songpol Chivapat, Pranee Chavalittumrong, Prapai Wongsinkongman, Chada Phisalpong, Anudep Rungsipipat Mar 2011

Chronic Toxicity Study Of Garcinia Mangostana Linn. Pericarp Extract, Songpol Chivapat, Pranee Chavalittumrong, Prapai Wongsinkongman, Chada Phisalpong, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

Ethanolic extracts from fruit pericarp of mangosteen (Garcinia mangostana L.) possess many biological andpharmacological activities. However, chronic toxicity study of ethanolic extract has never been investigated. Theobjective of this study was to evaluate the safety of 95% ethanolic extract from mangosteen pericarp in animal model.The oral administration of the extract was performed in 180 Wistar rats randomly allocated to six groups, each of15/sex. Group 1 was control group receiving distilled water. Group 2 to 6 were treatment groups receiving the extractat the doses of 10, 100, 500, 1000 and 1000 mg/kg/day for six months respectively. The last group was assigned …


Immunodetection Of Insulin-Like Growth Factor Binding Proteins (Igfbps) In The Sera Of Different Animal Species, Romana Masnikosa, Ivona Baricevic, Dragana Lagundzin, Olgica Nedic Jan 2011

Immunodetection Of Insulin-Like Growth Factor Binding Proteins (Igfbps) In The Sera Of Different Animal Species, Romana Masnikosa, Ivona Baricevic, Dragana Lagundzin, Olgica Nedic

Turkish Journal of Veterinary & Animal Sciences

Knowledge regarding the insulin-like growth factor (IGF) system and the methods for the detection and quantitation of IGF binding proteins (IGFBPs) is greater for the human species than for other animal species. This is mainly because antibodies specifically raised against nonhuman IGFBPs are rarely commercially available. We aimed to investigate which of the antibodies directed against human IGFBPs could be used for the detection of IGFBPs present in other species. We used commercially available antibodies, raised against human IGFBP-1 through IGFBP-4, in order to recognize the corresponding IGFBPs in the sera of different animal species: human, bovine, equine, porcine, ovine, …


Chronic Toxicity Study Of Kaempferia Parviflora Wall Ex. Extract, Songpol Chivapat, Pranee Chavalittumrong, Aimmanas Attawish, Anudep Rungsipipat Dec 2010

Chronic Toxicity Study Of Kaempferia Parviflora Wall Ex. Extract, Songpol Chivapat, Pranee Chavalittumrong, Aimmanas Attawish, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

K. parviflora is a medicinal plant possessing high potential for development of various health products. The objective of this chronic toxicity study was to investigate the safety of ethanolic extract of chronic Kaempferia parviflora Wall ex Bak in Wistar rats. The animals were randomly divided into five groups, twenty four rats each (12 males and 12 females). Three treatment groups were orally administered with K. parviflora extract at doses of 5, 50 and 500 mg/kg/day for six months respectively, which were equivalent to 1, 10 and 100 times of human use, while two control groups were orally given with distilled …


The Efficacy Of Pure Natural Repellents On Rat Responses Using Circular Open Field, Sarinee Kalandakanond-Thongsong, Suwaporn Daendee, Boonrit Thongsong, Vivat Chavananikul Dec 2010

The Efficacy Of Pure Natural Repellents On Rat Responses Using Circular Open Field, Sarinee Kalandakanond-Thongsong, Suwaporn Daendee, Boonrit Thongsong, Vivat Chavananikul

The Thai Journal of Veterinary Medicine

Rodent infestation is considered as one of the major pest problems since it is not only acting as a health hazard, but also causes damages to household, agricultural fields and even transportation business. The use of rodenticides is a common approach to rodent control, however, most rodenticides are poisonous to man. Therefore, using natural extracts as a rat repellent may be a better alternative. In the current study, various natural extracts were experimented whether they could repel a rat when testing in the behavioral model, a circular open field. The tested substances were wintergreen oil, chilli, peppermint oil, bergamot oil …


Toxicity Testing Of Flowers Of Neem Tree (Azadirachta Indica A. Juss), Piengchai Kupradinun, Anong Tepsuwan, Nopsarun Tanthasri, Nuntana Meesiripan, Sirirat Tunsakul, Warayupa Tompat, Yaninee Jarratwisarutporn, Wannee R. Kusamran Mar 2010

Toxicity Testing Of Flowers Of Neem Tree (Azadirachta Indica A. Juss), Piengchai Kupradinun, Anong Tepsuwan, Nopsarun Tanthasri, Nuntana Meesiripan, Sirirat Tunsakul, Warayupa Tompat, Yaninee Jarratwisarutporn, Wannee R. Kusamran

The Thai Journal of Veterinary Medicine

Flowers of neem (Azadirachta indica A. Juss) possess a strong cancer chemopreventive potential in rats as well as antimicronucleus formation in mice. However, toxicity testing has never been evaluated. This study was aimed to determine its acute and subacute toxicities in Wistar rats. Acute toxicity was conducted by feeding methanol extract of neem flowers (MENF) suspending in 20% propylene glycol at 6, 9 and 12 g/kg bw. On the other hand, subacute toxicity testing was carried out by feeding MENF suspending in 0.5% tragacanth at 150, 750 and 1,500 mg/kg bw for 90 consecutive days. Hematology, blood chemistry and histopathology …


Effects Of A Beta Adrenergic Antagonist Combined With Vasodilators On Cardiac Arrhythmia In Ischemic-Induced Rat Hearts, Promporn Raksaseri, Wuthichai Klomkleaw, Suwanakiet Sawangkoon Jun 2007

Effects Of A Beta Adrenergic Antagonist Combined With Vasodilators On Cardiac Arrhythmia In Ischemic-Induced Rat Hearts, Promporn Raksaseri, Wuthichai Klomkleaw, Suwanakiet Sawangkoon

The Thai Journal of Veterinary Medicine

Sixty male Spraque-Dawley rats were used in this study. The hearts were isolated and placed on a constant-pressure Langendorff apparatus, and perfused with only Krebs-Henseleit buffer (control), atenolol, atenolol plus prazosin, and atenolol plus salbutamol. After that the left anterior descending artery was occluded for 8 minutes and reperfused for 30 minutes to induce cardiac arrhythmias. The left ventricular pressure and coronary flow rate were monitored, and ECGs were recorded to evaluate cardiac arrhythmias. The administration of atenolol (β1-adrenergic antagonist) combined with prazosin (α1-adrenergic antagonist) prolonged RR intervals whereas that of atenolol combined with salbutamol (β2-adrenergic antagonist) did not prolong …


The Effects Of Qualitative And Quantitative Protein Malnutrition On Cecal Microbiota In Wistar Rats With Or Without Neutrophil Suppression, Hali̇l Biyik, Muharrem Balkaya, Hümeyra Ünsal, Cengi̇z Ünsal Jan 2005

The Effects Of Qualitative And Quantitative Protein Malnutrition On Cecal Microbiota In Wistar Rats With Or Without Neutrophil Suppression, Hali̇l Biyik, Muharrem Balkaya, Hümeyra Ünsal, Cengi̇z Ünsal

Turkish Journal of Veterinary & Animal Sciences

Gastrointestinal microbiota has been claimed to be affected by the diet consumed. In this study the effects of severe qualitative and quantitative protein malnutrition on cecal microbiota of male Wistar rats with or without neutrophil depletion were investigated. A total of 43 animals were divided randomly into 7 groups. Group I (control) was given a complete chow diet, groups II, III and IV received almost protein free (N-free) diet and groups V, VI and VII received a 20% gelatin containing diet for 35 days ad libitum. In addition, groups I, II and V were given physiologic saline, groups III and …


The Effects Of Fenfluramine On Blood And Tissue Serotonin (5-Hydroxytryptamine) Levels In Rats, İbrahi̇m Pi̇ri̇nçci̇, Ahmet Ateşşahi̇n, İzzet Karahan Jan 2005

The Effects Of Fenfluramine On Blood And Tissue Serotonin (5-Hydroxytryptamine) Levels In Rats, İbrahi̇m Pi̇ri̇nçci̇, Ahmet Ateşşahi̇n, İzzet Karahan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the effect of fenfluramine (FEN) administered at single and repeated doses on brain, stomach and plasma serotonin (5-Hydroxytryptamine, 5-HT) levels in rats. FEN was injected intraperitoneally at single doses (1, 3 and 10 µmol kg^{-1}) and given orally at repeated doses (1.25, 5 and 10 mg kg^{-1}) for 21 days. The tissues and plasma samples were collected 20, 40, 80 and 160 minutes after the single dose administrations and 4 h after the last dose administration in repeated experiments. In addition, the rats treated with the dose of 1.25 mg kg^-1 were …


Effects Of Phenobarbital On Serum And Liver Paraoxonase And Arylesterase Activities In Rats, Ahmet Ateşşahi̇n, İzzet Karahan, İbrahi̇m Pi̇ri̇nçci̇, M. Feri̇t Gürsu, Funda Gülcü Jan 2004

Effects Of Phenobarbital On Serum And Liver Paraoxonase And Arylesterase Activities In Rats, Ahmet Ateşşahi̇n, İzzet Karahan, İbrahi̇m Pi̇ri̇nçci̇, M. Feri̇t Gürsu, Funda Gülcü

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the effects of phenobarbital, administered orally and intraperitoneally over different periods, on paraoxonase, arylesterase, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities and total protein levels in the serum and liver. Thirty-six rats, 16-18 weeks old and weighing 240-260 g, were used. Phenobarbital was applied both orally and intraperitoneally at a dose of 100 mg/kg. Paraoxonase and arylesterase activities in serum and liver samples were measured spectrophotometrically using paraoxon and phenylasetate substrates, respectively. Although no significant changes were determined in serum paraoxonase and arylesterase activities, liver paraoxonase and arylesterase activities and total protein …


6-Phosphogluconate Dehydrogenase: Purification, Characterization And Kinetic Properties From Rat Erythrocytes, Şükrü Beydemi̇r, Mehmet Çi̇ftçi̇, Hayrullah Yilmaz, Ömer İrfan Küfrevi̇oğlu Jan 2004

6-Phosphogluconate Dehydrogenase: Purification, Characterization And Kinetic Properties From Rat Erythrocytes, Şükrü Beydemi̇r, Mehmet Çi̇ftçi̇, Hayrullah Yilmaz, Ömer İrfan Küfrevi̇oğlu

Turkish Journal of Veterinary & Animal Sciences

In this paper, a simple and rapid method for the purification of 6-phosphogluconate dehydrogenase from rat erythrocytes together with an analysis of the kinetic behavior and some properties of the enzyme are considered. The purification steps comprised high-speed centrifugation, 20-50% ammonium sulfate precipitation and 2', 5'-ADP Sepharose 4B affinity gel chromatography. The yield was 78.4% and the specific enzyme activity was 5.15 EU/mg proteins. The molecular mass of the subunit was estimated to be 59,566 Da by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and native enzyme was found to be 111,000 Da by gel filtration column chromatography. The enzyme had an …


Morphometry Of The Lymph Nodes Excluding The Thoracic And Abdominal Cavity In Wistar Rats, İlknur Dabanoğlu, Hüsnü Erbay Bardakçioğlu, Fi̇gen Sevi̇l, Di̇dem Alp Jan 2004

Morphometry Of The Lymph Nodes Excluding The Thoracic And Abdominal Cavity In Wistar Rats, İlknur Dabanoğlu, Hüsnü Erbay Bardakçioğlu, Fi̇gen Sevi̇l, Di̇dem Alp

Turkish Journal of Veterinary & Animal Sciences

Ten male adult Wistar rats were used to obtain some measurements of the lymph nodes located in the head, neck, forelimb and hindlimb. The lymph nodes were marked by the injection of Evans Blue and were then dissected. In order to provide sufficient data for the morphometric properties of lymph nodes, the lymph nodes on each side were counted, and the longest and widest diameters of the lymph nodes were also measured. The mandibular lymph nodes were found to be higher in number in comparison with the other lymph nodes investigated. The largest was found in the axillary accessory lymph …


The Effects Of High Copper And Zinc On Some Hematological Parameters In Rats, Sebahat Aydemi̇r, Mukaddes Özcan Jan 2003

The Effects Of High Copper And Zinc On Some Hematological Parameters In Rats, Sebahat Aydemi̇r, Mukaddes Özcan

Turkish Journal of Veterinary & Animal Sciences

The effect of high levels of Zn and Cu supplemented into the diet on Zn and Cu levels in the liver, pancreas and serum, the number of red and white blood cells, hematocrit value, hemoglobin levels, superoxide dismutase (SOD) activity in red blood cells, live weight, and liver and pancreas weights were investigated. A total of 77 Wistar albino rats were used as the research material. Twelve of the rats were separated as the control group and the remaining 65 were divided into five experimental groups (D1, D2, D3, D4 and D5) with 13 rats in each. Then, 70 mg/kg/day …


The Effect Of Zinc On Body Weight And Some Serum Enzymes In Rats, Süleyman Korkut Tekeli̇, Nezi̇r Yaşar Toker, Fatma Tekeli̇ Jan 2002

The Effect Of Zinc On Body Weight And Some Serum Enzymes In Rats, Süleyman Korkut Tekeli̇, Nezi̇r Yaşar Toker, Fatma Tekeli̇

Turkish Journal of Veterinary & Animal Sciences

100 male Wistar albino rats were used in this study. After the adaptation period, the rats were divided into 5 equal groups. The experimental period lasted 22 days. Food and water were given ad libitum. 0 ppm, 100 ppm, 500 ppm, 1,000 ppm and 2,000 ppm zinc were given by nasogastric probe to the control, experiment-1, experiment-2, experiment-3 and experiment-4 groups respectively, on days 1, 6, 11, 16, and 21 of the experimental period. The animals were weighed and blood samples were taken on days 2, 7, 12, 17, 22 of the experimental period. AST, ALT and ALP levels in …


Investigation On The Situation Of Free And Bound Residues On Rats Fed Chicken Liver Containing Free And Bound Aflatoxin B_1 Residues, Mehmet Özdemi̇r Jan 2002

Investigation On The Situation Of Free And Bound Residues On Rats Fed Chicken Liver Containing Free And Bound Aflatoxin B_1 Residues, Mehmet Özdemi̇r

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study is to determine free and bound residues of 3H-aflatoxin B_1 given orally by a gavage at a single dose (1 mg/kg body weight) in the liver tissues of broilers, and to show whether they accumulate as free and bound aflatoxin B_1 residues in the tissues of liver and muscle of the rats that consumed the liver of those broilers. A total of 30 Ross PM-3 broilers and 49 male Sprague-Dawley rats were used in the study. Free aflatoxin B_1 residue in the liver tissues of the chicken treatment group after 12h was found to …


Morphological Adaptation Of Rat Skeletal Muscle To A Cold Environment, Fati̇me Geyi̇koğlu, Aysel Temelli̇, Abdullah Özkaral Jan 2002

Morphological Adaptation Of Rat Skeletal Muscle To A Cold Environment, Fati̇me Geyi̇koğlu, Aysel Temelli̇, Abdullah Özkaral

Turkish Journal of Veterinary & Animal Sciences

Male Sprague-Dawley rats (SD) were kept in medium between the postnatal 80^{th} and 130^{th} day while environmental temperature (from 20 to 5 ºC) and photoperiod were decreased gradually. The weight of the animals adapted to the cold and the weight and length of the external digitorum longus (EDL) muscle were measured. When compared with controls, in a cold environment the weight and fiber length of the muscle was reduced significantly. In addition, EDL muscle was separated into two (red and white) by using the succinic dehydrogenase (SDH) method and the percentage of fiber types and fiber diameters according to mitochondria …


Morphological And Microscopical Examination Of The Rat Brachial Plexus, Ahmet Uzun, Nureddi̇n Cengi̇z, Ahmet Kavakli, Saci̇de Karakaş Jan 2001

Morphological And Microscopical Examination Of The Rat Brachial Plexus, Ahmet Uzun, Nureddi̇n Cengi̇z, Ahmet Kavakli, Saci̇de Karakaş

Turkish Journal of Veterinary & Animal Sciences

The rat brachial plexus was studied morphologically and the average numbers of the fascicles were studied by light microscope. For this purpose, we used 30 Wistar albino adult rats (16 males, 14 females; 180-250 g). The rats were anaesthetized by urethane (0.6 ml/100 g body weight injected i.p.). The brachial plexus was prepared for the microdissection technique and was evaluated in terms of its formation. Sections of the origin level of roots (C_5, C_6, C_7, C_8 and Th_1) and the terminal branches (ulnar, median, radial (musculospiral), axillary (circumflex), and musculocutaneous nerves) were taken and the average numbers of fascicles were …


Antiinflammatory And Antipyretic Activity Of Polygonum Lapathifolium In Rats, Oya Keleş, Tülay Bakirel, Süleyman Şener, Handan Aydin, Keri̇m Alpinar Jan 2001

Antiinflammatory And Antipyretic Activity Of Polygonum Lapathifolium In Rats, Oya Keleş, Tülay Bakirel, Süleyman Şener, Handan Aydin, Keri̇m Alpinar

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to evaluate the pharmacologic effects of Polygonum lapathifolium L. (Polygonaceae), growing naturally in Turkey. The antiinflammatory activity of the ethanol extract of the root and rhizome of P. lapathifolium was evaluated using carrageenan-induced paw edema in rats. Intraperitoneal pretreatment with extract at doses ranging from 100 to 500 mg/kg significantly inhibited the acute phase of this experimental model of the inflammatory process in a dose-dependent manner. At maximal dose (500 mg/kg), it exhibited a significantly higher action when compared with those of other doses and was found to be 1.08 times as active as indomethacin. The …


Epidermal Growth Factor Receptor (Egfr) Immunolocalization In The Male Rat Reproductive Tract During Pre-And Postnatal Periods, Celal Kaloğlu, Hüseyi̇n Eray Bulut, Bi̇lge Onarlioğlu Jan 2000

Epidermal Growth Factor Receptor (Egfr) Immunolocalization In The Male Rat Reproductive Tract During Pre-And Postnatal Periods, Celal Kaloğlu, Hüseyi̇n Eray Bulut, Bi̇lge Onarlioğlu

Turkish Journal of Veterinary & Animal Sciences

A number of recent studies suggest the effects of several growth factors as well as genetic factors on the development and differentiation of the testes and excretory ducts. One of the most important growth factors affecting the development and differentiation of the above-mentioned organs may be epidermal growth factor (EGF). Therefore, the aim of the present study was to investigate the effects of EGF on the development of the testes and excretory ducts of male rats by determining the immunolocalization of EGF receptors in these organs during foetal and postnatal periods. To do this, testicular and excretory duct tissue samples …


Lectin And Peptide Expression In Nodose, Sphenopalatine And Superiorcervical Ganglia Of The Rat, Zafer Soygüder Jan 1999

Lectin And Peptide Expression In Nodose, Sphenopalatine And Superiorcervical Ganglia Of The Rat, Zafer Soygüder

Turkish Journal of Veterinary & Animal Sciences

The presence and distribution of Griffonia SimplicifoliaI-B4 (GSA I-B4) and Calcitonin Gene Related Peptide (CGRP) were studied in the nodose ganglion (NG) (inferior ganglion of the vagus nerve), sphenopalatine ganglion (SPG) and superior cervical ganglion (SCG) of the rat. GSA I-B4 labeling was found in all ganglia tested. Neither SPG nor SCG cell bodies stained with CGRP were undetectable. The pattern of the distribution of GSA I-B4 and CGRP labeled cells were quite similar in the nodose ganglion. They were found in the poles of the ganglion with some marginal labeling. Large numbers of GSA I-B4 and CGRP labeled cells …


The Effect Of Ultraviolet B (Uvb) Radiation On Some Electrolytes In Rats, Mukaddes Özcan, Göknur Akin, Ülker Çöteli̇oğlu, Murat Arslan Jan 1999

The Effect Of Ultraviolet B (Uvb) Radiation On Some Electrolytes In Rats, Mukaddes Özcan, Göknur Akin, Ülker Çöteli̇oğlu, Murat Arslan

Turkish Journal of Veterinary & Animal Sciences

In our research, the effect of UVB radiation on the amounts of Ca^{++}, inorganic P(Pi), K^+, Na^+, Mg^{++} , Cl^- in serums of 22 week- old Wistar-Albino rats was investigated. UVB radiation was applied to trial group animals at dosages used for treatment (4 J/ cm^2) for a period of 4 weeks.The blood samples were taken before the ray application as the control group. At the end of one month there was no change in the amounts of Pi, K^+, Mg^{++} but the level of serum -Ca^{++} increased significantly (P


Efficacy Of Moxidectin Against Aspiculuris Tetrapteraand Syphacia Murisinnaturally Infected Rats, Ayşen Gargili, Erkut Tüzer, Aynur Gülanber, İlker Efil, Vedat Keleş, Meltem Ulutaş, Müfit Toparlak Jan 1998

Efficacy Of Moxidectin Against Aspiculuris Tetrapteraand Syphacia Murisinnaturally Infected Rats, Ayşen Gargili, Erkut Tüzer, Aynur Gülanber, İlker Efil, Vedat Keleş, Meltem Ulutaş, Müfit Toparlak

Turkish Journal of Veterinary & Animal Sciences

In this study, 56 rats (Rattus norvegicus) naturally infected with both Aspiculuris tetraptera and Syphacia muris were used. The animals were randomly divided into the treatment (37 animals) and control (19 animals) groups. Moxidectin (Cydectin enj., %1 Moxidectin) at 0.2 mg/kgbw was given subcutaneously to the treatment group. The faeces of both groups were examined on days 0, 7, 14, 21, 28. The post-mortem examination of one animal from each group was performed to confirm the EPG results on days mentioned above. The percentage efficacy was measured on the basis of the reduction of the eggs. The eggs and adults …


The Effects Of Orally Ethanol Treatment On Serum, Liver And Kidney\Gamma-Gt, Alt And Ast Enzymes Activity And Serum Total Cholesterol And Lipid Levels In Rats, Ayla Özcan, Ahmet Mengi̇ Jan 1998

The Effects Of Orally Ethanol Treatment On Serum, Liver And Kidney\Gamma-Gt, Alt And Ast Enzymes Activity And Serum Total Cholesterol And Lipid Levels In Rats, Ayla Özcan, Ahmet Mengi̇

Turkish Journal of Veterinary & Animal Sciences

In this study, some blood, liver and kidney parameters of rats which were given alcohol orally were investigated at the end of 1st, 7th, 14th and 21st days. Forty male Wistar Albino rats (mean 196 g), 20 wk of age, were used as materials. Serum AST and \gamma-GT activities at the end of 1st day were significantly different between control and experimental groups from 40 rats studied. At the end of 7th, 14th and 21st days, the difference between two groups for AST and \gamma-GT activities in serum and liver were significant (p