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Articles 31 - 60 of 190

Full-Text Articles in Veterinary Medicine

Environmental Sampling Techniques For Herd-Level Surveillance Of Bovine Viral Diarrhea Virus, Jaden Carlson Aug 2019

Environmental Sampling Techniques For Herd-Level Surveillance Of Bovine Viral Diarrhea Virus, Jaden Carlson

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Control of Bovine Viral Diarrhea Virus (BVDV) relies on resource-intensive individual animal sampling to detect and remove persistently infected (PI) cattle. Herd-level surveillance tools would be useful for herds with unknown BVDV status and for monitoring herds with BVDV-free status. The overall objective of this thesis is to explore the viability of BVDV surveillance at a herd-level using samples collected without handling individual animals. The first objective was to determine the feasibility of using stable flies as a sampling tool to detect BVDV. The second objective was to determine the feasibility of using drinking water to detect BVDV. To accomplish …

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Evaluating The Epidemiology And Management Of Bovine Congestive Heart Failure, Adam Bassett Jun 2019

Evaluating The Epidemiology And Management Of Bovine Congestive Heart Failure, Adam Bassett

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

A form of congestive heart failure is increasingly reported as a cause of death in feedlot cattle located at moderate altitude (≤ 1,524 m.). Significant knowledge gaps exist in the epidemiology and management of this form of bovine congestive heart failure (BCHF) regarding frequency and timing of BCHF cases and ways in which beef cattle producers and veterinarians can mitigate this condition. These knowledge gaps present major barriers to understanding the mechanism of BCHF and mitigating the consequences of BCHF. The purpose of this thesis is to summarize current knowledge about BCHF, define gaps in knowledge for which more research …

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Serodiagnostic Potential Of Brucella Outer Membrane And Periplasmic Proteins, Aitbay Bulashev, Orken Akibekov, Zhanbolat Suranshiyev, Bakytkali Ingirbay, Saule Eskendirova Jan 2019

Serodiagnostic Potential Of Brucella Outer Membrane And Periplasmic Proteins, Aitbay Bulashev, Orken Akibekov, Zhanbolat Suranshiyev, Bakytkali Ingirbay, Saule Eskendirova

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the serological diagnostic potential of the Brucella recombinant outer membrane (rOMP25, rOMP31) and periplasmic proteins (rBP26, rSOD) in a comparative way using an indirect enzyme-linked immunosorbent assay (i-ELISA). Rabbit and/or mouse antibodies to Brucella whole cell and/or soluble protein preparations recognized all recombinant proteins used, which confirms the expression of target antigens in E. coli in active form. The recombinant proteins showed different antigenicity to antibodies of cattle kept on a brucellosis-affected (endemic) farm and/or a new focus of infection. Thus, the presence of anti-Brucella antibodies was confirmed by i-ELISA/rSOD in 79% …

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Molecular Detection Of Anaplasma Spp. In Blood And Milk Of Dairy Cattle In The Philippines, Angelito Pangan Dela Cruz, Remil Linggatong Galay, Kristina Andrea Claveria Sandalo, Flor Marie Immanuelle Rafallo Pilapil-Amante, Tetsuya Tanaka Jan 2019

Molecular Detection Of Anaplasma Spp. In Blood And Milk Of Dairy Cattle In The Philippines, Angelito Pangan Dela Cruz, Remil Linggatong Galay, Kristina Andrea Claveria Sandalo, Flor Marie Immanuelle Rafallo Pilapil-Amante, Tetsuya Tanaka

Turkish Journal of Veterinary & Animal Sciences

Anaplasmosis has become a major concern in the cattle industry throughout the world due to its great economic impact. The causative agents, Anaplasmaspecies, are primarily transmitted by ticks, occurring intracellularly within blood cells, with some species being zoonotic. In this study, the presence of Anaplasma spp. was investigated in the blood and milk of dairy cattle in the Philippines. Blood and milk samples were collected from 98 dairy cattle from selected farms in five provinces in the southern part of Luzon Island in the Philippines. After DNA extraction, a conventional PCR for the control gene actin was performed, followed by …

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Snake Envenomation In Two Cattle: Clinical/Laboratory Aspects And Treatment Using Equine-Derived Antivenin Of Viperidae, Nuri̇ Altuğ, Cafer Tayer İşler Jan 2019

Snake Envenomation In Two Cattle: Clinical/Laboratory Aspects And Treatment Using Equine-Derived Antivenin Of Viperidae, Nuri̇ Altuğ, Cafer Tayer İşler

Turkish Journal of Veterinary & Animal Sciences

In this case report, specific antivenin treatment for Viperidae snake envenomation in two cattle was defined for the first time. The diagnosis of snake envenomation was made according to the history and clinical findings. Clinical examination revealed systemic (tachycardia, dyspnea, cyanosis, hypersalivation, ruminal hypotony) and local (swelling of the leg and head) findings depending on the location of snake bites. Laboratory analysis determined granulocytic leukocytosis, monocytosis, decreased iron binding capacity, and increased CK and LDH enzyme activities. In the specific treatment of cases, equine-derived polyvalent antivenin containing antitoxic immunoglobulins against Viperidae species was administered. Additionally, 5% dextrose, antibiotics, dexamethasone, antihistamines, …

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Morphometric Analysis Of The Skulls Of Domestic Cattle (Bos Taurus L.) And Water Buffalo (Bubalus Bubalis L.) In Turkey, Ermi̇ş Özkan, Abu Bakar Siddiq, Ki̇fayet Oya Kahveci̇oğlu, Muhsi̇n Öztürk, Vedat Onar Jan 2019

Morphometric Analysis Of The Skulls Of Domestic Cattle (Bos Taurus L.) And Water Buffalo (Bubalus Bubalis L.) In Turkey, Ermi̇ş Özkan, Abu Bakar Siddiq, Ki̇fayet Oya Kahveci̇oğlu, Muhsi̇n Öztürk, Vedat Onar

Turkish Journal of Veterinary & Animal Sciences

A total of 20 domestic cattle (Bos taurus L.) and 15 water buffalo (Bubalis bubalis L.) skulls were analyzed in this study. All of the specimens belonged to female individuals. Using a total of 27 craniometric measurements from each of the skulls, 9 indices were calculated. Although there were statistically significant differences between the linear measurements of the skulls of both species, while calculating the indices with their ratios, the values of the measurements of the facial area were determinative in the craniology. Among these indices, the facial index 1 value was statistically significant (P < 0.01) in the comparison of these two species. On the other hand, while considering the orbit and foramen magnum measurements, in contrast with the transversal measurement, the height was more determinant for their index and form.

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Phylogenetic Relationships Of Native Turkish Cattle Breeds Using Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Müge Doğan, Zafer Bulut, Mehmet Ni̇zamlioğlu, Ayşe Işik, Aysun Çamlidağ, Vahdetti̇n Altunok Jan 2019

Phylogenetic Relationships Of Native Turkish Cattle Breeds Using Microsatellite Markers, Yusuf Özşensoy, Ercan Kurar, Müge Doğan, Zafer Bulut, Mehmet Ni̇zamlioğlu, Ayşe Işik, Aysun Çamlidağ, Vahdetti̇n Altunok

Turkish Journal of Veterinary & Animal Sciences

A total of 20 microsatellite DNA markers were used for genetic characterization and determination of phylogenetic relationships of native cattle breeds of Turkey, including the Anatolian Grey (AG), Anatolian Black (AB), South Anatolian Red (SAR), East Anatolian Red (EAR), Southern Anatolian Yellow (SAY), and Zavot (ZAV). DNA samples were isolated from 271 blood samples using an organic method. Amplified polymerase chain reaction products were separated by capillary electrophoresis and genotypes were determined for 20 microsatellites. A total of 269 different alleles were determined. The lowest (7.80) and highest (10.80) mean allele numbers were observed for the ZAV and SAY populations, …

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Optimized Oral Supplementation Of Vitamins Improves Feed Intake And Rumen Microbial Protein Synthesis In Deoni Cows, Ashwin Kandathil, Srinivas Bandla Jan 2019

Optimized Oral Supplementation Of Vitamins Improves Feed Intake And Rumen Microbial Protein Synthesis In Deoni Cows, Ashwin Kandathil, Srinivas Bandla

Turkish Journal of Veterinary & Animal Sciences

The present study was conducted to optimize oral doses of fat- and water-soluble vitamins in cattle using in vitro gas production model and to ascertain their effects on dry matter intake (DMI), nutrient digestibility, and rumen microbial protein (RMBP) synthesis. Doses optimized using the in vitro model were 3.66, 6.78, 16.3, 41.1, 3.87, 0.323, 4.12, and 0.055 mg/kg DMI, respectively, for thiamine, riboflavin, niacin, pantothenic acid, pyridoxine, biotin, folic acid, and B12 and 500 IU/kg DMI for vitamin K. Thirty-two adult, nonproducing Deoni cows (375 ± 13.4 kg body weight, BW) were randomly divided into four equal groups (n = …

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Electrocardiogram And Serum Cardiac Biomarkers Changes In Fmd In Cattle, Ali Abbas Nikvand, Seyedeh Missagh Jalali, Mohammad Nouri, Alireza Ghadrdan Mashhadi, Soroush Hassanpour Amirabadi Jan 2019

Electrocardiogram And Serum Cardiac Biomarkers Changes In Fmd In Cattle, Ali Abbas Nikvand, Seyedeh Missagh Jalali, Mohammad Nouri, Alireza Ghadrdan Mashhadi, Soroush Hassanpour Amirabadi

Turkish Journal of Veterinary & Animal Sciences

This study was designed to evaluate serum and electrocardiographic (ECG) changes in cattle with foot-and-mouth disease (FMD). Thirty-two cows with clinical signs of FMD and 13 healthy cows were randomly enrolled. After blood sampling, ECG was performed on 23 of the 32 ill cows. Serum circulatory troponin I (cTnI), CPK, LDH, and CK-MB activities were determined in the patient and control groups. Arrhythmias including ventricular tachycardia (VT), sinus tachycardia (ST), atrial fibrillation (AF), and premature ventricular complex (PVC) were seen in 4, 4, 2, and 1 of the patients, respectively. The patients had higher cTnI levels than the control group …

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Phenotypic And Genotypic Investigation Of The Heavy Metal Resistance In Escherichia Coli Isolates Recovered From Cattle Stool Samples, Ni̇sa Si̇pahi̇, Emre Karakaya, Serkan İki̇z Jan 2019

Phenotypic And Genotypic Investigation Of The Heavy Metal Resistance In Escherichia Coli Isolates Recovered From Cattle Stool Samples, Ni̇sa Si̇pahi̇, Emre Karakaya, Serkan İki̇z

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to examine resistance against cadmium (Cd$^{+2}$), copper (Cu$^{+2}$), lead (Pb$^{+2}$), mercury (Hg$^{+2}$), and manganese (Mn$^{+2}$) in Escherichia coli isolates recovered from cattle stool using phenotypic (agar dilution) and genotypic [polymerase chain reaction (PCR)] methods. In addition, the isolates were genotyped via enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Escherichia coli was isolated and identified from the 100 stool samples that were examined in the study. It was determined phenotypically that all isolates were sensitive to mercury; 97% of them showed resistance to cadmium; 69% showed resistance to copper; 24% showed resistance to lead; …

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Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan Dec 2018

Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan

Animal and Food Sciences Faculty Publications

Bovine mammary gland biopsies allow researchers to collect tissue samples to study cell biology including gene expression, histological analysis, signaling pathways, and protein translation. This article describes two techniques for biopsy of the bovine mammary gland (MG). Three healthy Holstein dairy cows were the subjects. Before biopsies, cows were milked and subsequently restrained in a cattle chute. An analgesic (flunixin meglumine, 1.1 to 2.2 mg/kg of body weight) was administered via jugular intravenous [IV] injection 15-20 min prior to biopsy. For standing sedation, xylazine hydrochloride (0.01-0.05 mg/kg of body weight) was injected via the coccygeal vessels 5-10 min before the …

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Detection And Characterization Of Extended-Spectrum Beta-Lactamase Genes(Bla$_{Tem}$ And Bla$_{Shv}$) Among Beta-Lactam-Resistant Fecal Coliforms Of Dairy Cattle From Chhattisgarh, India, Nidhi Rawat, Fateh Singh, Sadanand Devidas Hirpurkar, Chandrahas Sannat, Nitin Eknath Gade Jan 2018

Detection And Characterization Of Extended-Spectrum Beta-Lactamase Genes(Bla$_{Tem}$ And Bla$_{Shv}$) Among Beta-Lactam-Resistant Fecal Coliforms Of Dairy Cattle From Chhattisgarh, India, Nidhi Rawat, Fateh Singh, Sadanand Devidas Hirpurkar, Chandrahas Sannat, Nitin Eknath Gade

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to isolate the antimicrobial-resistant enterobacteria from cattle feces and to identify the extended-spectrum beta-lactamase (ESBL) and carbapenemase gene among them. A total of 70 cattle fecal swab samples were collected from Durg, Chhattisgarh, India, and cultured in nutrient broth. Bacterial isolation recovered 120 gram-negative bacteria. Antimicrobial susceptibility testing showed 90 (75%) coliform isolates (66 Escherichia coli, 20 Klebsiella sp., and two each of Enterobacter sp. and Citrobacter sp.) resistant to amoxicillin, amoxicillin-clavulanic acid, cefixime, ceftazidime, imipenem, or meropenem. Bacterial isolates showed the highest resistance rate for ceftazidime (61.6%), followed by cefixime and amoxicillin-clavulanic acid …

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Morphological Diagnosis Of Johne?S Disease: A Case Report In Cattle, Siddharth Gautam, Chitra Joshi, Amol Gurav, Chandrakanta Jana, Anil Kumar Sharma Jan 2018

Morphological Diagnosis Of Johne?S Disease: A Case Report In Cattle, Siddharth Gautam, Chitra Joshi, Amol Gurav, Chandrakanta Jana, Anil Kumar Sharma

Turkish Journal of Veterinary & Animal Sciences

The present case report deals with the morphological (gross and histopathological) and histochemical characterization of Johne?s disease in a cow. The carcass was presented in a hidebound condition with sunken eyes and with a history of persistent and nonresponsive diarrhea. Necropsy revealed generalized gelatinization of the visceral and peripheral fat. Thorough gross examination unveiled major pathological changes in the digestive tract. The gallbladder was distended, with severely thickened walls and blood mixed with bile. The small intestine was severely congested and the mesenteric lymph nodes were enlarged. The opening of the small intestine showed marked thickening of the intestinal walls, …

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Effects Of Enzymatically Hydrolyzed Yeast Supplementation And Supplementation Frequency On Immune Parameters, Performance, And Digestibility Among Periparturient Beef Cows And Calves, Janine Swartz Jan 2018

Effects Of Enzymatically Hydrolyzed Yeast Supplementation And Supplementation Frequency On Immune Parameters, Performance, And Digestibility Among Periparturient Beef Cows And Calves, Janine Swartz

Electronic Theses and Dissertations

Effects of enzymatically hydrolyzed yeast (EHY) and supplementation frequency (FREQ) on immune parameters among beef cows and calves was evaluated. Eighty multiparous (parity = 4.2 ± 0.3) cows were fed a common brome hay-based diet (CP = 8.0 ± 0.17%). Cows were blocked by expected calving date and stratified by body condition score (BCS) before random assignment of treatment. Beginning 88 ± 5 d prior to parturition, cows were provided 1 kg daily or 3 kg every 3 d of a soybean hull-based supplement (CP = 34.0, % of DM) that contained 0 or 3 g/kg EHY. The daily supplement …

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Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska Jan 2018

Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska

Turkish Journal of Veterinary & Animal Sciences

The main aim of this study was to identify the association between the single nucleotide polymorphism (SNP) in the bovine interleukin-17A (IL-17A) gene and mastitis resistance. Additionally, the connection between the identified polymorphism and the somatic cell score (SCS) was tested. Two hundred and six Polish Holstein?Friesian cows were genotyped using the PCR-RFLP method. One SNP mutation (c.126G>A) was identified within the coding sequence of the analyzed gene. Results of logistic regression analysis displayed no significant association between the IL-17A genotype and clinical mastitis occurrence (Wald chi-square test = 0.74; P = 0.69). The GLM analysis demonstrated that genotype …

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Prevalence And Pathology Of Bovine Coccidiosis In Faisalabad District, Pakista, Riaz Hussain, Fazal Mahmood, Ahrar Khan, Khalid Mehmood Sep 2017

Prevalence And Pathology Of Bovine Coccidiosis In Faisalabad District, Pakista, Riaz Hussain, Fazal Mahmood, Ahrar Khan, Khalid Mehmood

The Thai Journal of Veterinary Medicine

The study aimed to investigate the presence of coccidial oocysts and pathological changes in a dairy herd with the history of coccidial outbreak in Faisalabad District. Fecal samples were collected from 541 animals, including cattle (379) and buffaloes (162), and examined for protozoal infection using flotation techniques. Results revealed 2.9% presence of coccidial oocysts in the samples collected. No significant difference was found in coccidial oocysts count in species of the cattle (2.3%) and buffaloes (4.3%), sex and age group. Necropsy revealed no gross lesions in thoracic organs, however, severe hemorrhages and necrotic ulcers were present in the cecum and …

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Comparison Of Four Ultrasonographic Approaches For The Diagnosis Ofacquired Reticular Diaphragmatic Hernia In Bovidae, Ashwani Kumar, Vandana Sangwan, Jitender Mohindroo, Narinder Singh Saini, Simrat Sagar Singh Jan 2017

Comparison Of Four Ultrasonographic Approaches For The Diagnosis Ofacquired Reticular Diaphragmatic Hernia In Bovidae, Ashwani Kumar, Vandana Sangwan, Jitender Mohindroo, Narinder Singh Saini, Simrat Sagar Singh

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to look at four ultrasonographic approaches that diagnose acquired reticular diaphragmatic hernia (RDH) using either left or right caudal thoracic approaches (4th/5th intercostal space) and compare them to those made more cranially (1st/2nd intercostal space). Each sonographic observation was made in 27 unsedated, standing animals (22 buffaloes and 5 cattle) that were surgically confirmed for RDH. Using the right caudal thoracic approach, the herniated reticulum was seen as medial to the thoracic wall at a depth of 3-5 cm and adjacent to the cardiac silhouette. With the left caudal thoracic approach, the herniated reticulum …

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Detection Of High Levels Of Somatic Cells In Milk On Farms Equippedwith An Automatic Milking System By Decision Trees Technique, Beata Sitkowska, Dariusz Piwczynski, Joanna Aerts, Magdalena Kolenda, Serkan Özkaya Jan 2017

Detection Of High Levels Of Somatic Cells In Milk On Farms Equippedwith An Automatic Milking System By Decision Trees Technique, Beata Sitkowska, Dariusz Piwczynski, Joanna Aerts, Magdalena Kolenda, Serkan Özkaya

Turkish Journal of Veterinary & Animal Sciences

The purpose of the study was to use decision trees to predict increased levels of somatic cells in cow's milk. The material for the study comprised data collected in 2012-2014 from five farms in Poland equipped with an automatic milking system. Data on 803 Polish Holstein-Friesian cows were collected. In order to predict somatic cell count data mining techniques were used to build a graphical model of a decision tree. This study found that the most important factors to anticipate an elevated somatic cell count in cow's milk are milk conductivity, lactation stage, and lactation (primiparous and multiparous cow groups), …

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Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut Jan 2017

Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

Bovine skin and teat papillomas were examined for immunohistochemical expression of bovine papillomavirus (BPV), proliferating antigens (PCNA, Ki-67), CD3+ T lymphocytes, and some of the type III intermediate filaments. Papillomas showed epidermal hyperplasia, koilocytes, and hyperkeratosis with prominent dermal connective tissue. Immunohistochemistry found the presence of BPV antigens in the nuclei of granulosa cells. PCNA and Ki-67 revealed positive nuclear staining in the dermis and epidermis. Perivascular cuffing in the dermis predominantly consisted of CD3+ T cells. Immunoreaction to vimentin was strong in the connective tissue of the dermis. $\alpha$SMA positivity was mainly in the media of vessels. Smooth muscle …

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Identification And Genetic Characterization Of Bovine Parainfluenzavirus Type 3 Genotype C Strain Isolated From Cattle In Western China, Xiaobo Wen, Jinhua Sun, Yao Zhang, Si Cao, Xuhua Ran, Hongbo Ni Jan 2017

Identification And Genetic Characterization Of Bovine Parainfluenzavirus Type 3 Genotype C Strain Isolated From Cattle In Western China, Xiaobo Wen, Jinhua Sun, Yao Zhang, Si Cao, Xuhua Ran, Hongbo Ni

Turkish Journal of Veterinary & Animal Sciences

Bovine parainfluenza virus type 3 (BPIV3) is a leading respiratory pathogen of cattle and is often implicated as a causative pathogen of bovine respiratory disease complex (BRDC). Three genotypes (BPIV3a-c) have been tentatively classified based on genetic and phylogenetic analyses. A BPIV3c isolate, designated NX49, was isolated using the Madin-Darby bovine kidney cell culture from cattle in western China, where feedlot cattle with severe respiratory disease symptoms were observed. Hemagglutination assay demonstrated that NX49 had low agglutination activity against guinea pig erythrocytes, whereas SD0835, the first BPIV3c strain reported in China, showed high agglutination activity. Phylogenic analysis indicated that NX49 …

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A Survey Of Ixodid Ticks Feeding On Cattle And Molecular Detectionof Coxiella Burnetii From Ticks In Southeast Iran, Omid Ghashghaei, Saeid Reza Nourollahi Fard, Mohammad Khalili, Hamid Sharifi Jan 2017

A Survey Of Ixodid Ticks Feeding On Cattle And Molecular Detectionof Coxiella Burnetii From Ticks In Southeast Iran, Omid Ghashghaei, Saeid Reza Nourollahi Fard, Mohammad Khalili, Hamid Sharifi

Turkish Journal of Veterinary & Animal Sciences

Ticks are the main hematophagous arthropods and obligatory ectoparasites that are considered a vector of serious pathogens for animals and humans. The aims of the present survey were to assess the prevalence and diversity of ticks in cattle in Southeast Iran and to determine Coxiella burnetii in the ticks. From May 2014 to April 2015, 583 ticks were collected randomly from 257 cattle. Nested trans-PCR was performed to detect C. burnetii. The overall prevalence of hard tick infestation on cattle was 56.8%. In total, seven different species were identified: Rhipicephalus sanguineus sensu lato (32.6%), Hyalomma anatolicum (28.8%), Hyalomma excavatum (19%), …

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Challenges And Prospects Of The Embryo Transfer Industry From A European Standpoint, Michel Thibier Dec 2016

Challenges And Prospects Of The Embryo Transfer Industry From A European Standpoint, Michel Thibier

The Thai Journal of Veterinary Medicine

In vivo derived (IVD) and in vitro produced (IVP) embryo transfers are respectively the 2nd and 3rd generations of the reproductive biotechnologies. Commercial application of these technologies began in the seventies for the former and in the eighties for the latter. After 40 years, they have clearly proved their sustainability to the livestock industry. The present article deals with bovine embryos only. The numbers of embryos collected or produced and transferred worldwide are reported in the first part. About one million bovine embryos are transferred each year. This clearly shows the significant impact they have on the cattle industry. The …

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Bovine And Ovine Schistosomiasis: Prevalence And Associated Host Factors In Selected Sites Of South Achefer District, Northwest Ethiopia, Yirsaw Kerie, Zewdu Seyoum Dec 2016

Bovine And Ovine Schistosomiasis: Prevalence And Associated Host Factors In Selected Sites Of South Achefer District, Northwest Ethiopia, Yirsaw Kerie, Zewdu Seyoum

The Thai Journal of Veterinary Medicine

Schistosomiasis is a snail-borne trematode infection in man and animals in tropical and subtropical countries. It is an economically important disease caused by several Schistosoma species and results in economic losses through mortality and morbidity from severe infection and from long-term effect of moderate- and long-standing chronic infection. This study was conducted from November 2013 to April 2014 to determine the prevalence and potential host-related risk factors of bovine and ovine schistosomiasis in selected sites of South Achefer district, northwest Ethiopia. About 532 faecal samples were collected from randomly selected cattle and sheep from three purposively selected peasant associations. The …

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Trophoblast Major Histocompatibility Complex Class I Expression Is Associated With Immune-Mediated Rejection Of Bovine Fetuses Produced By Cloning, Heloisa M. Rutigliano, Aaron J. Thomas, Amanda Wilhelm, Benjamin R. Sessions, Brady A. Hicks, Donald H. Schlafer, Kenneth L. White, Christopher J. Davies Aug 2016

Trophoblast Major Histocompatibility Complex Class I Expression Is Associated With Immune-Mediated Rejection Of Bovine Fetuses Produced By Cloning, Heloisa M. Rutigliano, Aaron J. Thomas, Amanda Wilhelm, Benjamin R. Sessions, Brady A. Hicks, Donald H. Schlafer, Kenneth L. White, Christopher J. Davies

Animal, Dairy, and Veterinary Science Faculty Publications

Trophoblast cells from bovine somatic cell nuclear transfer (SCNT) conceptuses express major histocompatibility complex class I (MHC-I) proteins early in gestation, and this may be one cause of the significant first-trimester embryonic mortality observed in these pregnancies. MHC-I homozygous-compatible (n = 9), homozygous-incompatible (n = 8), and heterozygous-incompatible (n = 5) SCNT pregnancies were established. The control group consisted of eight pregnancies produced by artificial insemination. Uterine and placental samples were collected on Day 35 ± 1 of pregnancy, and expression of MHC-I, leukocyte markers, and cytokines were examined by immunohistochemistry. Trophoblast cells from all SCNT pregnancies expressed MHC-I, while …

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Comparison Of Protein Phosphatase Inhibition Assay With Lc-Ms/Ms For Diagnosis Of Microcystin Toxicosis In Veterinary Cases, Caroline E. Moore, Jeanette Juan, Yanping Lin, Cynthia L. Gaskill, Birgit Puschner Mar 2016

Comparison Of Protein Phosphatase Inhibition Assay With Lc-Ms/Ms For Diagnosis Of Microcystin Toxicosis In Veterinary Cases, Caroline E. Moore, Jeanette Juan, Yanping Lin, Cynthia L. Gaskill, Birgit Puschner

Veterinary Diagnostic Laboratory Faculty Publications

Microcystins are acute hepatotoxins of increasing global concern in drinking and recreational waters and are a major health risk to humans and animals. Produced by cyanobacteria, microcystins inhibit serine/threonine protein phosphatase 1 (PP1). A cost-effective PP1 assay using p-nitrophenyl phosphate was developed to quickly assess water and rumen content samples. Significant inhibition was determined via a linear model, which compared increasing volumes of sample to the log-transformed ratio of the exposed rate over the control rate of PP1 activity. To test the usefulness of this model in diagnostic case investigations, samples from two veterinary cases were tested. In August …

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Cla And Epa Inhibit Lps-Induced Prostaglandin Release From Bovine Endometrial Cells Through An Nf-?B-Dependent Signaling Mechanism, Lokenga Badinga, Mehmet Şükrü Gülay, Alan Ealy Jan 2016

Cla And Epa Inhibit Lps-Induced Prostaglandin Release From Bovine Endometrial Cells Through An Nf-?B-Dependent Signaling Mechanism, Lokenga Badinga, Mehmet Şükrü Gülay, Alan Ealy

Turkish Journal of Veterinary & Animal Sciences

Although their action in antagonizing arachidonic acid metabolism is a key antiinflammatory effect of conjugated linoleic acid (CLA) and omega-3 polyunsaturated fatty acids (n-3 PUFA), these molecules have other antiinflammatory effects that might occur upstream of altered eicosanoid production. We examined the effects of two CLA isomers (c9,t11 and t10,c12 isomers), n-3 PUFA (EPA and DHA), and ΚNF-ΚB inhibitor (ammonium pyrrolidine dithiocarbamate (PDTC)) on lipopolysaccharide (LPS)-induced secretion of prostaglandins (PGE2 and PGF2a) by bovine endometrial (BEND) cells. LPS increased PGE2 and PGF2a concentrations in BEND cell-conditioned media in a concentration- and time-dependent manner. The c9,t11 CLA isomer and EPA decreased …

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Mast Cell Typing In Aortic Onchocerciasis And The Immunohistochemicaldemonstration Of Wolbachia Bacteria, Rahşan Yilmaz, Melek Koçak, Zafer Özyildiz, İsmai̇l Şah Harem Jan 2016

Mast Cell Typing In Aortic Onchocerciasis And The Immunohistochemicaldemonstration Of Wolbachia Bacteria, Rahşan Yilmaz, Melek Koçak, Zafer Özyildiz, İsmai̇l Şah Harem

Turkish Journal of Veterinary & Animal Sciences

In this study, the presence of endosymbiotic Wolbachia bacteria was determined by immunohistochemical methods in 8 out of 43 paraffin-embedded bovine aortic tissues that were macroscopically and microscopically diagnosed with aortic onchocerciasis in the archive of the Harran University Faculty of Veterinary Medicine's Department of Pathology. By means of the enzyme histochemical and immunohistochemical staining methods, it was ascertained that the tryptase-containing subtype of mast cells (MCT) was involved in the inflammatory response in the bovine aortae with onchocerciasis. The aim of this study was to elucidate the pathogenesis of aortic onchocerciasis lesions caused by Onchocerca armillata in Turkey and …

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Mycoplasma Infections In Dairy Cattle Farms In Turkey, Zafer Sayin, Asli Sakmanoğlu, Uçkun Sai̇t Uçan, Ali̇ Uslu, Hasan Hüseyi̇n Hadi̇mli̇, Zeki̇ Aras, Özgür Özdemi̇r, Osman Ergani̇ş Jan 2016

Mycoplasma Infections In Dairy Cattle Farms In Turkey, Zafer Sayin, Asli Sakmanoğlu, Uçkun Sai̇t Uçan, Ali̇ Uslu, Hasan Hüseyi̇n Hadi̇mli̇, Zeki̇ Aras, Özgür Özdemi̇r, Osman Ergani̇ş

Turkish Journal of Veterinary & Animal Sciences

Mycoplasmas cause the most severe and economically costly diseases of cattle throughout the world. In this study, Mycoplasma species were isolated from calves and cows with suspected mycoplasmosis in Holstein dairy cattle farms within 7 geographical regions of Turkey between May 2010 and December 2015. Mycoplasma infections were positive in 17 (80.9%) of 21 dairy cattle farms and the overall percentage was calculated as 32.1%. The highest isolation rate occurred in the Southeastern Anatolia Region (42.8%), and the lowest was observed in the Mediterranean Region (19.6%). In total, 172 Mycoplasma spp. were isolated from samples. Using PCR analysis, 149 (87.6%) …

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Molecular Identification Of Bovine Papillomaviruses In Dairy And Beef Cattle: First Description Of Xi- And Epsilonpapillomavirus In Turkey, Veysel Soydal Ataseven, Özgür Kanat, Yaşar Ergün Jan 2016

Molecular Identification Of Bovine Papillomaviruses In Dairy And Beef Cattle: First Description Of Xi- And Epsilonpapillomavirus In Turkey, Veysel Soydal Ataseven, Özgür Kanat, Yaşar Ergün

Turkish Journal of Veterinary & Animal Sciences

In the current study, 23 papilloma/tumor-like samples obtained from cattle having clinical lesions and 9 blood samples collected from healthy-appearing cattle in Turkey were examined for bovine papillomavirus (BPV) DNA using the degenerate primers FAP59/64, and the different types of BPV were distinguished by type-specific primer sets. Furthermore, histopathological studies of papillomavirus were performed. A total of 7 BPV types (BPVs 1, 2, 3, 4, 6, 8, 9), including genera Deltapapillomavirus, Xipapillomavirus, and Epsilonpapillomavirus, were identified. In all samples, BPV-1 was the most common genotype (90.6%). Overall, coinfections were determined in 26 of the examined samples (81.3%), and coinfection with …

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Comparison Of Hematological Parameters Of Brucella-Seronegative Naturallyinfected Cattle With Bvdv, Bhv-1, And Bhv-4, Muhammet Eren Aslan, Ahmet Kürşat Azkur, Emel Biyikli, Serkal Gazyağci, İbrahi̇m Sözdutmaz Jan 2016

Comparison Of Hematological Parameters Of Brucella-Seronegative Naturallyinfected Cattle With Bvdv, Bhv-1, And Bhv-4, Muhammet Eren Aslan, Ahmet Kürşat Azkur, Emel Biyikli, Serkal Gazyağci, İbrahi̇m Sözdutmaz

Turkish Journal of Veterinary & Animal Sciences

Abortion, neonatal deaths, and infertility cause important production losses in the livestock industry. Many viral infections are responsible for changes in hematological values of animals. This study aimed to find whether there is any difference in blood parameters of cattle that are naturally infected with BVDV, BHV-1, and BHV-4 and Brucella-seronegative. For this purpose, 401 whole-blood samples collected from cattle were analyzed by ELISA and PCR/RT-PCR to detect specific antibodies and antigens for BVDV, BHV-1, and BHV-4. Hematological analysis and results were compared between naturally infected BVDV, BHV-1, and BHV-4 groups and a control group. Results showed a statistically significant …

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