Veterinary Medicine Commons^™

Factors Affecting Treatment Outcome Of Canine Transmissible Venereal Tumor: A Retrospective Study, Pansawut Sudjaidee, Suppawiwat Ponglowhapan, Padet Tummaruk, Kaywalee Chatdarong

The Thai Journal of Veterinary Medicine

Canine transmissible venereal tumor (TVT) is a contagious neoplastic disease commonly seen in tropical and subtropical areas in the countries where the control of stray dog population is poor. This study aimed to summarize the current disease status and evaluate factors affecting treatment outcome to vincristine sulfate, which is the chemotherapeutic drug of choice. Studied factors were divided into three categories; general background (i.e. age, gender, breed), specific descriptions (i.e. tumor diameter, tumor site, season of the year), and treatment details (number of vincristine shots, meteorological parameters). One hundred and fifty-seven medical records were included with 100 achieved complete treatments …

Low-Dose Cyclophosphamide And Piroxicam Inhibit Growth, Migration, And Invasion Of Canine Oral Malignant Melanoma Cell Line, Nan Choisunirachon, Kota Yoshida, Kohei Saeki, Yuiko Tanaka, Manabu Mochizuki, Ryohei Nishimura, Nobuo Sasaki, Takayuki Nakagawa

The Thai Journal of Veterinary Medicine

The effect of low-dose chemotherapy on tumor motility of canine oral malignant melanoma has been investigated on an in vitro model using low-dose cyclophosphamide and piroxicam. Cyclophosphamide, at a concentration of 0.1 mg/mL in culture medium, significantly inhibited tumor cell migration/invasion through trans-well assays and suppressed both ERK and NF-κB signaling, the latter of which was observed through IκB-α and P-IκBα expression. Furthermore, in combination with a COX-2 inhibitor, i.e. piroxicam (0.38 mg/mL in culture medium), cyclophosphamide revealed a potential anti-migration effect over the single piroxicam treatment on a wound assay. This result suggested that low-dose cyclophosphamide might have influence …

Effect Of Pcso-524 On Oa Biomarkers And Weight-Bearing Properties In Canine Shoulder And Coxofemeral Osteoarthritis, Kumpanart Soontornvipart, Natwadee Mongkhon, Korakot Nganvongpanit, Prachya Kongtawelert

The Thai Journal of Veterinary Medicine

This study was designed to compare the therapeutic benefits of a compound of omega-3 fatty acids from the New Zealand green-lipped mussel (Perna canaliculus) (PCSO-524) and omega-3 fatty acids in fish oil on clinical outcomes and osteoarthritis biomarkers (chondroitin sulfate WF6 epitope) in 66 dogs that had osteoarthritis (OA); 39 dogs with OA hip joints, 15 dogs with OA shoulder joints and 12 dogs with OA shoulder and hip joints. The animals were presented at the Small Animal Hospital, Faculty of Veterinary Science, Chulalongkorn University. The dogs were allocated into two groups randomly. One group received PCSO-524 (n = 33) …

Prevalence Of Disorders Recorded In Cavalier King Charles Spaniels Attending Primary-Care Veterinary Practices In England, Jennifer F. Summers, Dan G. O'Neill, David B. Church, Peter C. Thomson, Paul D. Mcgreevy, David C. Brodbelt

Epidemiology Collection

Background: Concerns have been raised over breed-related health issues in purebred dogs, but reliable prevalence estimates for disorders within specific breeds are sparse. Electronically stored patient health records from primary-care practice are emerging as a useful source of epidemiological data in companion animals. This study used large volumes of health data from UK primary-care practices participating in the VetCompass animal health surveillance project to evaluate in detail the disorders diagnosed in a random selection of over 50% of dogs recorded as Cavalier King Charles Spaniels (CKCSs). Confirmation of breed using available microchip and Kennel Club (KC) registration data was attempted. …

Prevalence Of And Risk Factors For Degenerative Mitral Valve Disease In Dogs Attending Primary‐Care Veterinary Practices In England, M. Mattin, A. Boswood, D. B. Church, J. López-Alvarez, P. D. Mcgreevy, D. G. O'Neill, P. C. Thomson, D. C. Brodbelt

Epidemiology Collection

Background

To date, epidemiological studies on degenerative mitral valve disease (DMVD) in dogs have largely reported referral caseloads or been limited to predisposed breeds. Analysis of primary‐care data to identify factors associated with DMVD would help clinicians identify high‐risk individuals and improve understanding.

Objectives

To estimate the prevalence of and identify risk factors for DMVD in dogs attending primary‐care veterinary practices in England.

Animals

Cases were identified within the electronic patient records of 111,967 dogs attending 93 practices. Four hundred and 5 dogs were diagnosed with DMVD (diagnosed cases) and a further 3,557 dogs had a heart murmur (HM) consistent …

Prevalence And Risk Factors For Mast Cell Tumours In Dogs In England, Stephanie J.W. Shoop, Stephanie Marlow, David B. Church, Kate English, Paul D. Mcgreevy, Anneliese J. Stell, Peter C. Thomson, Dan G. O'Neill, David C. Brodbelt

Epidemiology Collection

Background

Mast cell tumour (MCT) appears to be a frequent tumour type in dogs, though there is little published in relation to its frequency in dogs in the UK. The current study aimed to investigate prevalence and risk factors for MCTs in dogs attending English primary-care veterinary practices.

Methods

Electronic patient records from practices participating in the VetCompass animal surveillance project between July 2007 and June 2013 were searched for MCT diagnosis. Various search terms and standard diagnostic terms (VeNom codes) identified records containing MCT diagnoses, which were evaluated against clinical criteria for inclusion to the study. MCT prevalence for …

The Effects Of Prostatic Fluid On Functional Characteristics Of Cooled Canine Semen, Reto Fritsche

LSU Master's Theses

The objectives of this study were to investigate concentration dependent effects of canine prostatic fluid (PF) on in vitro seminal parameters of cooled canine semen. Sperm motility parameters, plasma membrane integrity and stability, acrosome integrity and DNA fragmentation were measured after the addition of 0%, 10%, 25%, or 50% PF to extended semen of fertile dogs. Assessments were made at 0 h pre-cooling, at 24, and 48 h of cooled storage (4 °C), and after freezing and thawing followed by incubation (37 °C) at 0, 4 and 24 h. Our hypothesis was that lower dilutions of canine semen with PF …

Proliferative Index And Microvessel Density As Potential Prognostic Markers Of Canine Oral Malignant Melanomas, Nan Choisunirachon, Yuiko Tanaka, Kohei Saeki, Nobuo Sasaki, Ryohei Nishimura, Takayuki Nakagawa

The Thai Journal of Veterinary Medicine

Several reports have indicated the prognostic significance of the proliferative index (PI) and microvessel density (MVD) in canine oral malignant melanomas (MM). However, a correlation between the PI and MVD of oral MM in dogs has never been elucidated. Here, we evaluated the expression of Ki-67 and CD31 using double immunofluorescence staining to investigate the correlation of PI with MVD in 37 spontaneous canine oral MMs. No correlation was found between the MVD and PI values or between those and any clinicopathologic findings. However, a correlation was found between the PI value and both clinical stage (p = 0.0453) and …

Retrospective Study Of Computed Tomographic Characterization Of Canine Oral Malignant Melanoma In 24 Dogs, Nan Choisunirachon, Lee-Shuan Lin, Yuiko Tanaka, Kohei Saeki, Reina Fujiwara, Ryohei Nishimura, Takayuki Nakagawa

The Thai Journal of Veterinary Medicine

Clinical and computed tomographic (CT) information from 24 histopathological diagnosed canine oral malignant melanomas (MMs) was retrospectively investigated. The CT appearance on canine oral MM from each patient was evaluated, including both bone and soft tissue windows and pre- and post-contrast enhancement. Among the 24 dogs, canine oral MM was highly presented in small breeds, especially in miniature dachshunds older than 10 years. The gingiva was the most commonly affected site, followed by palatine, mucosal, and lingual areas. The location of the primary tumor in the oral arcade was statistically significant associated with bone invasion such as osteolysis. Canine oral …

Approaches To Canine Health Surveillance, Dan G. O'Neill, David B. Church, Paul D. Mcgreevy, Peter C. Thomson, Dave C. Brodbelt

Epidemiology Collection

Effective canine health surveillance systems can be used to monitor disease in the general population, prioritise disorders for strategic control and focus clinical research, and to evaluate the success of these measures. The key attributes for optimal data collection systems that support canine disease surveillance are representativeness of the general population, validity of disorder data and sustainability. Limitations in these areas present as selection bias, misclassification bias and discontinuation of the system respectively. Canine health data sources are reviewed to identify their strengths and weaknesses for supporting effective canine health surveillance. Insurance data benefit from large and well-defined denominator populations …

The Effect Of Oviductal Cells On In Vitro Maturation Of Canine Oocytes In Different Culture Media, Si̇nem Özlem Engi̇nler, Asi̇ye İzem Sandal, Özen Banu Özdaş, Ramazan Arici, Ezgi̇ Ertürk, Eli̇f Merve Çinar, Israa Faris Mohammed, Alper Baran, Çağatay Tek, Mehmet Can Gündüz

Turkish Journal of Veterinary & Animal Sciences

The aims of this study were to investigate the effect of oviductal cells on in vitro maturation (IVM) of canine oocyte in Tissue Culture Medium 199 (TCM-199) or synthetic oviductal fluid (SOF) supplemented with bovine serum albumin (BSA) or fetal calf serum (FCS) and to compare the maturation rates of oocytes from the diestrus and anestrus stages. Following ovariohysterectomy, 13 pairs of ovaries were collected from bitches in anestrus (n = 10) or diestrus (n = 3) and oocytes were harvested by slicing. The oviducts were flushed with TCM-199 containing 10% FCS and were scraped and squeezed into a tube …

Cloning Efficiency Of Canine Mesenchymal Stem Cells Isolated From Bone Marrow Of Femoral Head And Subcutaneous Adipose Tissue, Nada Tanamai, Sirirak Chantakru, Monchanok Vijarnsorn

The Thai Journal of Veterinary Medicine

Most recently, adipose tissue (AD) has become an alternative source for mesenchymal stem cells (MSCs) instead of the invasive method of bone marrow (BM) aspiration both in human and canine. In this study, we compared MSCs derived from adipose tissue (AD-MSCs) and bone marrow (BM-MSCs) regarding morphology and cell yield for instant usage by using the standard protocol of counting colony forming unit-fibroblast (CFU-F). MSCs from both sources showed fibroblast-like morphology and formed colonies termed as CFU-F after culturing in plastic surface for 10 days. The colony number per mononuclear cells (MNCs) and the colony number per adherent cells derived …

Optimization Of Feline Adipose-Derived Multipotent Stromal Cell Isolation And Canine Cranial Cruciate Ligament Regeneration With Intra-Articular Adipose-Derived Multipotent Stromal Cells, Nan Zhang

LSU Doctoral Dissertations

Adult multipotent stromal cells (MSCs) have become popular within veterinary sciences for direct administration and tissue regeneration due to their differentiation, trophic and immunosuppressive properties. However, current isolation and expansion techniques provide a heterogeneous population with other types of cells, which may affect the efficiency and efficacy of treatments. The progenitor properties and function need to be further evaluated in vitro prior to in vivo application. The first part of this dissertation was to identify the optimal MSC source for general canine orthopedic applications such as intra-articular injection and joint tissue regeneration. Based on side-by-side comparisons of cell doublings, colony …

Computed Tomographic Assessment Of Canine Arytenoid Lateralization, James Richard Wignall

LSU Master's Theses

Unilateral arytenoid lateralization is a commonly performed surgical treatment for laryngeal paralysis in dogs. It involves fixing the moveable arytenoid cartilage to the thyroid (TAL) or cricoid (CAL) cartilage or both (CTAL). This increases the area of the rima glottidis (RGA), to allow reduced airway pressure and laryngeal resistance in vitro and ameliorates clinical signs in vivo. It may also increase the patient’s predisposition for aspiration pneumonia, which occurs in around 20% of clinical patients. No surgical technique has been correlated with clinical outcome or risk of aspiration pneumonia. Objective analysis of the effects of surgery on the three dimensional …

Role Of Matrix Metalloproteinases In Animals, Sariya Asawakarn, Tanong Asawakarn

The Thai Journal of Veterinary Medicine

Matrix metalloproteinases are a family of calcium-dependent and zinc-dependent endopeptidases, which excrete from many cell types. These enzymes can hydrolyze extracellular matrix proteins. The most important inhibitor of MMPs is tissue inhibitors of metalloproteinases (TIMPs). The imbalance between MMPs and TIMPs causes pathological condition in human and animals. This review focuses on structural, role and several reports of MMPs in horse, dog, cat, fish and avian. Over production of MMPs is associated with tissue destruction and erosion of cartilage in equine osteoarthritis, especially MMP-2 and MMP-9. In canine and feline, many evidences reported about MMPs and cancer such as MMP-2 …

Treatment Of Canine Transmissible Venereal Tumor Using Vincristine Sulfate Combined With L-Asparaginase In Clinical Vincristine-Resistant Cases: A Case Report, Pansawut Pansawut, Patrakrit Theewasutrakul, Sirichai Techarungchaikul, Suppawiwat Ponglowhapan, Kaywalee Chatdarong

The Thai Journal of Veterinary Medicine

Three female mongrel dogs were cytologically diagnosed as transmissible venereal tumor (TVT) and had clinically developed resistance to vincristine treatment. One dog was treated with four treatments of 10,000 IU/m2 body surface area of L-asparaginase combined with 0.025 mg/kg body weight of vincristine sulfate every two weeks while this combination was administered to the others once a week for four weeks and only vincristine sulfate once a week for four treatments. Both treatments resulted in a complete remission. Side effects such as gastrointestinal upset, diarrhea, depression, and decrease in appetite were observed in the dogs administered with the later protocol. …

Differentiation Potentials Of Canine Bone Marrow Mesenchymal Stem Cells, Theerawat Tharasanit, Nawapen Phutikanit, Chalika Wangdee, Kumpanart Soontornvipart, Sasijaras Tantrajak, Theerayuth Kaewamatawong, Junpen Suwimonteerabutr, Pitt Supaphol, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

Mesenchymal stem cells (MSCs) are multipotent cells that have characteristics of self-renewal anddifferentiation into various specific cell types, in particular mesodermal lineages. This study aimed at isolating,identifying and examining the differentiation capability of canine MSCs. Bone marrow aspirates were obtained from4 dogs. Putative MSCs were then cultured in MSC medium and subpassaged. At the 3rd to 5th passages, MSCs wereexamined for their morphology and doubling time. Two cell lines were examined for the expression of CD 34, CD 44and CD 90, using flow cytometry. The in vitro differentiation of these MSCs into mesodermal lineages (bone, cartilageand adipose tissues) and ectodermal …

The Effect Of A Long-Acting Gnrh Agonist (Deslorelin) On Estrogen Receptor Alpha (Erα) And Progesterone Receptor (Pr) Expressions In Canine Mammary Tissue, Sukanya Manee-In, Sayamon Srisuwatanasagul, Chainarong Lohachit, Junpen Suwimonteerabutr, Timothy E. Trigg, Sudson Sirivaidyapong

The Thai Journal of Veterinary Medicine

The objective of this study was to evaluate the expression of estrogen receptor alpha (ERα) and progesterone receptor (PR) in normal mammary tissues of bitches before and after the bitch was implanted with the GnRH agonist, deslorelin. Fifteen mature bitches with no pathological condition of the mammary gland, and during anestrus, were selected and divided into two groups: five bitches were implanted with placebo (placebo group); and the ten bitches were implanted with 9.4 mg deslorelin (deslorelin group). Mammary tissues were collected from all bitches before implantation and at 2 and 12 weeks after implantation. The expressions of ERα and …

Phenotypic Characterization Of Canine Cranial Cruciate Ligament Associated Synoviocytes, Sunil C. Vasanjee

LSU Master's Theses

Identification of synoviocytes surrounding the canine cranial cruciate ligament (CrCL) has not been investigated. Objectives 1) Develop and validate a technique to identify and quantify normal canine CrCL associated synoviocytes. 2) Compare synoviocyte phenotype proportions surrounding normal and abnormal canine CrCLs Design In vitro experimental Animals Cranial cruciate ligaments from 4 intact female and 6 intact adult male mixed-breed dogs (objective 1) and from 8 adult female hound dogs (objective 2) Methods Objective 1) Normal CrCLs - CD18 and HSP25 epitopes were colocalized using immunohistochemistry. Sagittal sections were quantified in the proximal, middle, and distal aspects of each section. Western …

Phacofragmentation And Aspiration In Canine Mature Cataract: Surgical Technique, Success Rate And Complications, Pranee Tuntivanich, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

Phacofragmentation and aspiration technique without intraocular lens implantation was reported in 26 mature cataractous dogs. Dogs included in this study were 13 Poodles, 6 crossbred dogs, 4 Cocker spaniels, 1 Shih-tzu, 1 West Highland white terrier and 1 Miniature pinscher. All dogs were clinically blinded from bilateral mature cataracts with positive dazzle reflex prior to the surgery. 24 dogs (92.31%) regained vision after the surgery which remained until the end of the study. Vitreous degeneration was found pre-operatively in one dog. Hyphema, displacement of lens into vitreous chamber, rupture of the posterior lens capsule and miosis were found intra-operatively in …

The Study Of The Radiographic Positionings Of The Canine Elbow For Identifying The Medial Coronoid Process, Suwicha Chuthatep, Kongkiat Srisuwatanasagul, Chalika Wangdee, Kumpanart Sunthornwipart, Surassanan Angkana

The Thai Journal of Veterinary Medicine

Twenty cadaver canine forelimbs, 9 right and 11 left, were used to evaluate the most effective radiographic position that can enhance the cranial aspect of the medial coronoid process (MCP) of the ulna. A lead pellet (1 mm) was buried to represent the area representing the cartilegenous fragment in the canine patient in the fragmented medial coronoid process (FMCP). The distance between the pellet and the radial surface and the superimposition of the humeral condyle silhouette on the pellet were used as parameters for analysis. The results showed that the disto45 medialproximolateral oblique (Di45M-PrLO) position with a 40 flexed elbow …

The Use Of Porcine Small Intestinal Submucosa In Ten Cases Of Canine Corneal Staphyloma, Nalinee Tuntivanich, Kumpanart Soontornwipart, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

Porcine small intestinal submucosa biomaterial was used to repair corneal staphyloma in 10 dogs. There was no evidence of leakage of aqueous humor and graft rejection following PSIS grafting. Anterior synechia was presented in 25-50% of the corneal area. Granulation tissue vanished within 50 days of the operation. Even though scar formation and anterior synechia were observed at the end of the study, all dogs had vision.

A Geometric Modeling Of Dog Intestine, Hüseyi̇n Yildiz, Kadri̇ Arslan, İhsani̇ye Coşkun, Bahri̇ Yildiz

Turkish Journal of Veterinary & Animal Sciences

In computer-assisted surgical applications, reconstruction of 3-D morphological peculiarities are required. Herein, we present a geometric modeling of the separate intestinal sections of the canine. The intestine of the dog was considered a tubular shape along a special curve. Seven male Turkish shepherd dogs were used for the modeling study. The length (cm) and diameter (mm) of the intestines were measured with digital calipers and formulated. These models were then compared to their original photographs. It was concluded that the geometric modeling and experimental work were consistent. These kinds of organ modeling techniques will enable medical lecturers to show 3-D …

Pubic Symphysiodesis For The Optional Treatment Of Canine Hip Dysplasia, Kanyada Mangklapruk, Kumpanart Soontornvipart, Atichat Brahmasa

The Thai Journal of Veterinary Medicine

Pubic symphysiodesis (PS) using a bone plate and screws was performed in 10 hip dysplastic dogs, diagnosed following clinical and radiographic evaluation. After resection of the cranial third of the pubic symphysis, the rest of the symphysis was fixed by a dynamic compression plate and screws. Animal gait, Norberg angle (NA), dorsal acetabular rim slope (DARS) and dorsal acetabular rim angle (DARA) were radiographically evaluated before, immedimtely after, and at 1 and 3 months after surgery. NA and DAR of all dogs were significantly (p<0.05) greater than the preoperative values, while DARS was less than the preoperative value. After surgery, 75% of the hip joints had no Ortolani signs, while all dogs regained normal gait within one week of the operation.

Urokinase And Urokinase Receptor In The Urinary Tract Of The Dog, Trina Racquel Bailey

LSU Master's Theses

Urokinase (uPA) and urokinase receptor (uPAR) are present in the urinary tract of people, mice, rats and cows. The presence of urokinase and urokinase receptor in the urinary tract of dogs has not been documented. Documenting the presence of uPA and uPAR in the urinary tract of healthy dogs will help determine the role these proteins play in pathology of the urinary tract in further research. Urine was collected from fifty-four healthy dogs. A fluorescence assay was developed using the fluorogenic substrate Z-Gly-Gly-Arg-AMC, HCl. This assay documented presence of active uPA-like protein in concentrated urine. A direct sandwich ELISA assay …

Neoplasms Of Dogs In Bangkok, Anudep Rungsipipat, Boonmee Sunyasootcharee, Lek Ousawaphlangchai, Achariya Sailasuta, Roongroje Thanawongnuwech, Komkrich Teankum

The Thai Journal of Veterinary Medicine

2,425 neoplasms from dogs in Bangkok were submitted to the Department of Pathology, Chulalongkorn University, from January 1982 to December 2000 (a 19-year period) and were retrospectively studied to survey the prevalence of the different types of tumours. Histopathological diagnosis was based on the World Health Organization (WHO, 1974) classification of tumours in domestic animals. The prevalence of canine tumours was categorized into skin and soft tissue tumours, 55.05% (1,335 cases); mammary tumours, 28.29% (686 cases); reproductive tumours, 9.69% (235 cases); musculoskeletal tumours, 1.86% (45 cases); digestive tumours, 2.68% (65 cases); hematopoietic and lymphatic tumours, 1.94% (47 cases) and urinary …

Immunological, Hematological, And Serum Biochemical Effects Of High Level Dietary Fish Oil And Vitamin E Supplementation In The Dog, Casey J. Leblanc

LSU Doctoral Dissertations

Inflammation is a component of the innate immune response. However, severe or prolonged inflammation can be detrimental. Dietary n-3 fatty acid supplementation has proven clinical benefits in chronic inflammatory diseases, most likely due to reduced synthesis of inflammatory mediators and inhibition of lymphocyte proliferation and function. The purposes of this study were to characterize alterations in inflammatory mediator production, and lymphocyte proliferation, in dogs fed a diet consisting of 0.65% n-3 fatty acids (DMB) with an n-6:n-3 fatty acid ratio of 3.4:1. Fifteen dogs were randomly assigned to one of three dietary groups for twelve weeks. Group Sunflower oil received …

The Expression Of P53, C-Erbb-2 And C-Yes In Canine Skin Tumors, Anudep Rungsipipat, Susumu Tateyama, Yuichi Murakami, Kazuyuki Uchida

The Thai Journal of Veterinary Medicine

The exact role of the p53 tumor suppressor gene, c-erbB-2 and c-yes oncogenes in the development of canine skin tumors is still debatable. To elucidate the involvement of these oncogenes in canine skin carcinogenesis, we examined the expression of the protein in 60 skin tumor samples using avidin-biotin complex immunohistochemistry, with polyclonal antibodies directed against each protein, on formalinfixed, paraffin-embedded sections. Positive nuclear staining for p53 protein was observed in 12/60 cases (20%) which included, 6/15(40%) squamous cell carcinomas (SCC) and 4/8(50%) perianal gland tumors (PGT). Out of all the tumors examined, mixed membrane/ cytoplasmic staining of c-erbB-2 protein was …