Veterinary Medicine Commons^™

In Vitro Effect Of Recombinant Human Gonadotropins On Meiotic Competence Of Dog Oocytes, Kamber Demi̇r, Mi̇that Evecen, Ramazan Arici, Seli̇n Yağcioğlu, Nur Ersoy, Ni̇lhan Coşkun, Hatem Atalla, Kemal Ak, Sema Bi̇rler, Serhat Pabuccuoğlu

Turkish Journal of Veterinary & Animal Sciences

The reproductive biology of the domestic dog is unique among mammalian species; because of this, in vitro maturation (IVM) rate is still very low compared with other domestic animals in spite of attempts at improvement. The aim of this study was to consider the use of recombinant human gonadotropins as a replacement for pituitary gonadotropins for IVM of dog oocytes. A total of 845 cumulus-oocyte complexes were used in this study. To determine the effects of human recombinant gonadotropins, maturation medium was supplemented with two different concentrations (0.05 or 0.1 IU/mL) of pituitary (pFSH, pLH) and human recombinant (rhFSH and …

Cointegration Analysis Of Wild And Farmed Sea Bream And Sea Bass Prices In Turkey, Mehmet Saltuk Arikan, Ahmet Cumhur Akin, Mustafa Bahadir Çevri̇mli̇, Mustafa Agah Teki̇ndal

Turkish Journal of Veterinary & Animal Sciences

Market integration is an indicator of the extent to which different products are interrelated, and the presence of market integration between products suggests that they are substitutes. The objective of this study was to determine the market interaction between wild and farmed products of two fish species, namely sea bream and sea bass. For this purpose, the relationship between the producer price time series of cultured sea bream, wild sea bream, cultured sea bass, and wild sea bass in the period of 2009-2017 was tested using the cointegration analysis technique. It was found that the wild sea bream and sea …

Predicting The Body Weight Of Balochi Sheep Using A Machine Learning Approach, Zil E. Huma, Farhat Iqbal

Turkish Journal of Veterinary & Animal Sciences

Various machine learning algorithms have been used to model and predict the body weight of rams of the Balochi sheep breed of Pakistan. The traditional generalized linear model along with regression trees, support vector machine, and random forests methods have been used to develop models for the prediction of the body weight of animals. The independent variables (inputs) include the body (body length, heart girth, withers height) and testicular (scrotal diameter, scrotal circumference, scrotal length, and testicular length) measurements of 131 male sheep 2-36 months of age. The performance of the models is assessed based on evaluation criteria of mean …

Morphometric Analysis Of The Skulls Of Domestic Cattle (Bos Taurus L.) And Water Buffalo (Bubalus Bubalis L.) In Turkey, Ermi̇ş Özkan, Abu Bakar Siddiq, Ki̇fayet Oya Kahveci̇oğlu, Muhsi̇n Öztürk, Vedat Onar

Turkish Journal of Veterinary & Animal Sciences

A total of 20 domestic cattle (Bos taurus L.) and 15 water buffalo (Bubalis bubalis L.) skulls were analyzed in this study. All of the specimens belonged to female individuals. Using a total of 27 craniometric measurements from each of the skulls, 9 indices were calculated. Although there were statistically significant differences between the linear measurements of the skulls of both species, while calculating the indices with their ratios, the values of the measurements of the facial area were determinative in the craniology. Among these indices, the facial index 1 value was statistically significant (P < 0.01) in the comparison of these two species. On the other hand, while considering the orbit and foramen magnum measurements, in contrast with the transversal measurement, the height was more determinant for their index and form.

Molecular Detection Of Anaplasma Spp. In Blood And Milk Of Dairy Cattle In The Philippines, Angelito Pangan Dela Cruz, Remil Linggatong Galay, Kristina Andrea Claveria Sandalo, Flor Marie Immanuelle Rafallo Pilapil-Amante, Tetsuya Tanaka

Turkish Journal of Veterinary & Animal Sciences

Anaplasmosis has become a major concern in the cattle industry throughout the world due to its great economic impact. The causative agents, Anaplasmaspecies, are primarily transmitted by ticks, occurring intracellularly within blood cells, with some species being zoonotic. In this study, the presence of Anaplasma spp. was investigated in the blood and milk of dairy cattle in the Philippines. Blood and milk samples were collected from 98 dairy cattle from selected farms in five provinces in the southern part of Luzon Island in the Philippines. After DNA extraction, a conventional PCR for the control gene actin was performed, followed by …

Snake Envenomation In Two Cattle: Clinical/Laboratory Aspects And Treatment Using Equine-Derived Antivenin Of Viperidae, Nuri̇ Altuğ, Cafer Tayer İşler

Turkish Journal of Veterinary & Animal Sciences

In this case report, specific antivenin treatment for Viperidae snake envenomation in two cattle was defined for the first time. The diagnosis of snake envenomation was made according to the history and clinical findings. Clinical examination revealed systemic (tachycardia, dyspnea, cyanosis, hypersalivation, ruminal hypotony) and local (swelling of the leg and head) findings depending on the location of snake bites. Laboratory analysis determined granulocytic leukocytosis, monocytosis, decreased iron binding capacity, and increased CK and LDH enzyme activities. In the specific treatment of cases, equine-derived polyvalent antivenin containing antitoxic immunoglobulins against Viperidae species was administered. Additionally, 5% dextrose, antibiotics, dexamethasone, antihistamines, …

Molecular Detection And Phylogenetic Analysis Of The Honeybee (Apis Mellifera) Sacbrood Virus In Turkey, Gülnur Kalayci, Abdurrahman Anil Çağirgan, Kemal Pekmez, Buket Özkan, Murat Kaplan

Turkish Journal of Veterinary & Animal Sciences

Sacbrood virus (SBV) is widely distributed in honeybees. It is an infectious and contagious disease, which affects both the larvae and adult stages of honeybees (Apis mellifera). This study reports the detection of sacbrood virus in Turkey in 2017. Phylogenetic analysis based on partial sequences encoding the polyprotein gene was carried out in SBV-infected bees from four different apiaries. Phylogenetic analysis revealed noteworthy results. Although European-South American, Asian, and Korean genotypes have a maximum of 5.3% variance with each other, the variation rates between them and the Turkish genotype were 10.4%, 11.4%, and 12.5%, respectively. Sequences of Turkish isolates did …

Ethnoveterinary Medicine In Turkey: A Comprehensive Review, Zeynep Büşra Erarslan, Şükran Kültür

Turkish Journal of Veterinary & Animal Sciences

Today, traditional societies throughout the world possess a wealth of ethnoveterinary knowledge, which they have accumulated during prolonged interaction with nature. Turkey has ample resources of ethnoveterinary medicines as evident from its rich floral diversity and traditional botanical knowledge from the past. This review aims to compile herbal remedies used in ethnoveterinary medicine in Turkey with comprehensive usage information and to compare our findings with pharmacological studies. The botanical, family, and local names; used parts; preparation methods; administration/dosage and duration of the treatment; and ailments treated/therapeutic effects were presented by screening ethnobotanical and ethnoveterinary studies. The most cited plant families, …

Effect Of Various Antioxidants And Their Combinations On Bull Semen Cryopreservation, Şükrü Güngör, Ayhan Ata, Muhammed Enes İnanç, John P. Kastelic

Turkish Journal of Veterinary & Animal Sciences

Our objective was to evaluate the effects of adding 50 mM trehalose (TRE), 1 mM cysteine (CYS), or 400 µg catalase (CAT), individually or in combinations, to a Tris-based semen extender and determine the effects on fresh, chilled-equilibrated, and frozen-thawed bull sperm. Ejaculates from three Holstein bulls (1-3 years old) were pooled, divided into six equal portions, and put into a Tris-based extender supplemented with no additive (control), TRE, CYS, CAT, TRE + CYS, or TRE + CYS + CAT. In chilled-equilibrated semen, motility was highest in CAT but lowest in the control (P < 0.05). In thawed semen, motility was lower (P < 0.05) in CAT than in all other groups, which were not different. Based on a hypoosmotic swelling test (HOS test), TRE, CYS, and their combinations had higher (P < 0.05) percentages of sperm with an intact plasma membrane than the control or CAT groups. The TRE group had the least (P < 0.05) morphologically abnormal sperm. Plasma membrane and acrosomal integrity (PMAI) and high mitochondrial membrane potential (HMMP) values were highest in TRE and TRE + CYS and were lower (P < 0.05) in the control. Furthermore, HMMP was positively correlated with PMAI and HOS tests, but negatively correlated with major + minor abnormalities (r = 0.66, 0.242, and -0.349). In conclusion, the addition of TRE, with or without CYS, to a Tris-based semen extender improved several aspects of the frozen-thawed quality of bull semen. However, addition of CAT, by itself or in combination with other additives, did not protect bull sperm against cryopreservation-induced defects.

Phylogenetic Analyses Of P32, Rpo30, Gpcr, Orf117, And Kelch-Like Genes Of Tunisian Sheeppox Virus Isolates, Jihene Lachheb, Aicha Turki, Houssem Mastour, Jihene Nsiri, Imen El Behi, Imen Labri, Abdeljelil Ghram

Turkish Journal of Veterinary & Animal Sciences

Sheeppox is an economically important disease of small ruminants caused by the sheeppox virus (SPPV). Sheeppox virus (SPPV), goatpox virus, and lumpy skin disease virus are capripoxviruses, antigenically related but genetically distinct. Their differentiation requires analysis at a molecular level. Eleven sheeppox virus isolates from five different regions of Tunisia were collected between 2010 and 2016. SPPV was detected by real-time PCR using SYBR Green technology. Primers amplifying the entire Kelch-like gene were designed. Then the GPCR, RPO30, P32, ORF117, and Kelch-like genes were molecularly analyzed to show the different specific lineage patterns of each species. Phylogenetic analyses revealed three …

Negative Effects Of Occurrence Of Clinical Mastitis From Calving To End Of The Voluntary Waiting Period On Reproduction In Holstein Cows, Seçki̇n Salar, Oğuz Çalişici, Duygu Çalişici, Doğukan Özen, Ayhan Baştan

Turkish Journal of Veterinary & Animal Sciences

The objective was to evaluate the effects of clinical mastitis (CM) on reproductive performance in high-milk?producing cows before the service period. The data of Holstein Friesian cows (n = 550) in a commercial farm was retrospectively evaluated. The diagnosis of CM was made by the presence of visible abnormalities in milk or symptoms of inflammation of one or more of the quarters. Cows were checked to detect CM prior to each milking. Calving to first service interval (CFSI), calving to conception interval (CCI), and services per conception (SC) were evaluated as reproductive parameters. Calving to first service interval (83.68 ± …

On-Farm Diagnosis Of Latent Respiratory Failure In Calves, Anton Chernitskiy, Sergey Shabunin, Tatiana Kuchmenko, Vladimir Safonov

Turkish Journal of Veterinary & Animal Sciences

The goal of this study was to develop a method for the diagnosis of latent respiratory failure in calves with bovine respiratory disease (BRD) in farm conditions. The study involved 48 calves of the red-motley Holstein breed aged 14-28 days, with no previously experienced BRD, no dyspnea at rest, and a lack of anemia. Group I consisted of 24 calves with spontaneous or induced cough and WI clinical score of ≥5. Group II included 24 calves without spontaneous and induced cough and WI clinical score of ≤3. The determination of respiratory rate (RR), respiratory minute volume, and tidal volume in …

Neutralizing Antibody Titers Against Field Strains Of Bovine Viral Diarrhea Virus After Vaccination With Three Commercial Vaccines, Gi̇zem Alpay, Kadi̇r Yeşi̇lbağ

Turkish Journal of Veterinary & Animal Sciences

Modified killed vaccines against bovine viral diarrhea virus (BVDV) are used worldwide. In the present study, the cross-neutralization antibody responses against field strains from seven subgenotypes of BVDV-1 and one subgenotype of BVDV-2 were evaluated using sera obtained by three inactivated commercial vaccines. One vaccine contained both BVDV-1 and BVDV-2 strains, while the others contained only BVDV-1a. Three vaccine groups, each containing five calves, were vaccinated two times with 30-day intervals. The antibody titers were evaluated by virus neutralization assay. The monovalent vaccine induced the highest antibody titers. Significant levels of neutralizing antibody titers were maintained up to the last …

Effect Of Exercise On Some Minerals, Metabolites And Enzyme Activities In The Serum Of Trained Arabian Horses, Roya Pourmohammad, Mehrdad Mohri, Hesam Seifi, Kamran Sardari

Turkish Journal of Veterinary & Animal Sciences

The exercise-induced variations of some biochemical parameters including calcium (Ca), phosphorous (P), iron (Fe), copper (Cu), manganese (Mn), selenium (Se), zinc (Zn), sodium (Na), potassium (K), chlorine (Cl), glucose, urea, creatinine, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatine kinase (CK) were assessed in the serum of 25 Arabian horses. Blood samples were taken from the jugular vein 3 different times: before exercise, 5 h after exercise, and 18 h after exercise. Statistical analysis was performed using SAS (version 9.2; SAS Institute, Cary, NC, USA). Ca, Cl, glucose, and urea showed significant (P < 0.05) changes due to exercise. There was significant increase from before exercise to 5 h after exercise in urea concentrations while Ca and Cl concentrations significantly decreased. A significant ascending trend for Ca between 5 h and 18 h after exercise was also seen, while glucose and urea had a significantly descending trend. No significant time-based variations were observed for other variables. The results of the present study indicated that exercise could affect the value of some biochemical parameters, which could be used in future studies evaluating the health status of Arabian horses.

Study Of Retail Egg Supply Chain For Quality In Relation To Level Of Sanitization Andfarm Of Origin, Vinayananda Chilur Omkarappa, Mohamed Nadeem Fairoze, Madhavaprasad Chakkodabail Benakabhat, Kaustubh Bhave, Nagappa Karabasanavar

Turkish Journal of Veterinary & Animal Sciences

In the present cross-sectional study, eggs collected from retail outlets were analyzed for physicochemical and microbial quality. Comparisons were made between ?sanitized? (cleaned, sanitized, and retailed after packaging) and ?unsanitized? (not subjected to cleaning, sanitization, and packaging) retail table eggs that originated from ?commercial? and ?backyard? (eggs retailed loose without any cleaning, sanitization, or packaging) farms. A total of 1120 eggs collected from retail markets were analyzed for physicochemical (weight, shell thickness, shape, yolk index, albumen index, Haugh unit, color, and pH) and microbial (total viable count, and yeast and mold counts) characteristics. Eggs collected from retail markets were found …

Abdominal Ultrasonographic Findings In Dogs With Mammary Tumors: Association With Tumor Characteristics And Survival, Daniella Matos Da Silva, Angélica Inajá Juliani, Camila Lopes Ribeiro, Jair Rodini Engracia Filho, Simone Domit Guérios, Tilde Rodrigues Froes

Turkish Journal of Veterinary & Animal Sciences

Abdominal ultrasound is frequently performed as part of initial staging of canine mammary tumors. The aims of this study were to describe abdominal ultrasonographic findings present at diagnosis of mammary tumors in dogs and to assess their value for associations with histopathological results and treatment outcome. The medical records of 201 female dogs diagnosed with mammary tumors that had abdominal ultrasonography performed as part of their initial clinical evaluation were reviewed. Histopathological diagnosis of malignant mammary tumors was obtained in most cases (86%; 172 cases). One hundred and fifty-seven animals (78%) had some ultrasonographic abnormality. A total of 338 ultrasonographic …

Phenotypic Characterization And Differentiation Of Mesenchymal Stem Cells Originating From Adipose Tissue, Ece Çerçi̇, Hati̇ce Erdost

Turkish Journal of Veterinary & Animal Sciences

Stem cells are known as undifferentiated cells that are capable of divisions and self-renewal over an extended period. Adipose tissue-derived mesenchymal stem cells (ADSCs) are known to differentiate into many different organ and tissue cells. The purpose of this study was to characterize adipose tissue-derived mesenchymal stem cells in detail based on the expression status of certain surface markers and differentiation stages for clinical and laboratory applications. The migrating phase and mitotic property of the cells from adipose tissue were assessed under a microscope. Cell counting was performed during the first, second, and third passages. The cells were differentiated into …

Viral Emergence In Marine Mammals In The North Pacific May Be Linked To Arctic Sea Ice Reduction, Elizabeth Vanwormer, J. A.K. Mazet, A. Hall, V. A. Gill, P. L. Boveng, J. M. London, T. Gelatt, B. S. Fadely, M. E. Lander, J. Sterling, V. N. Burkanov, R. R. Ream, P. M. Brock, L. D. Rea, B. R. Smith, A. Jeffers, M. Henstock, M. J. Rehberg, K. A. Burek-Huntington, S. L. Crosby, J. A, Hammond, T. Goldstein

School of Veterinary and Biomedical Sciences: Faculty Publications

Climate change-driven alterations in Arctic environments can influence habitat availability, species distributions and interactions, and the breeding, foraging, and health of marine mammals. Phocine distemper virus (PDV), which has caused extensive mortality in Atlantic seals, was confirmed in sea otters in the North Pacific Ocean in 2004, raising the question of whether reductions in sea ice could increase contact between Arctic and sub-Arctic marine mammals and lead to viral transmission across the Arctic Ocean. Using data on PDV exposure and infection and animal movement in sympatric seal, sea lion, and sea otter species sampled in the North Pacific Ocean from …

Ehdv-2 Infection Prevalence Varies In Culicoides Sonorensis After Feeding On Infected White-Tailed Deer Over The Course Of Viremia, Sandra Y. Mendiola, Mary K. Mills, Elin Maki, Barbara S. Drolet, William C. Wilson, Roy D. Berghaus, David E. Stallknecht, Jonathan Breitenbach, D. Scott Mcvey, Mark G. Ruder

School of Veterinary and Biomedical Sciences: Faculty Publications

Epizootic hemorrhagic disease viruses (EHDVs) are arboviral pathogens of white-tailed deer and other wild and domestic ruminants in North America. Transmitted by various species of Culicoides, EHDVs circulate wherever competent vectors and susceptible ruminant host populations co-exist. The impact of variation in the level and duration of EHDV viremia in white-tailed deer (Odocoileus virginianus) on Culicoides infection prevalence is not well characterized. Here we examined how infection prevalence in a confirmed North American vector of EHDV-2 (Culicoides sonorensis) varies in response to fluctuations in deer viremia. To accomplish this, five white-tailed deer were experimentally infected with EHDV-2 and colonized C. …

Continuous Low-Intensity Ultrasound Attenuates Il-6 And Tnfα-Induced Catabolic Effects And Repairs Chondral Fissures In Bovine Osteochondral Explants, Neety Sahu, Hendrik J. Viljoen, Anuradha Subramanian

Department of Chemical and Biomolecular Engineering: Faculty Publications

Background: Cartilage repair outcomes are compromised in a pro-inflammatory environment; therefore, the mitigation of pro-inflammatory responses is beneficial. Treatment with continuous low-intensity ultrasound (cLIUS) at the resonant frequency of 5 MHz is proposed for the repair of chondral fissures under pro-inflammatory conditions.

Methods: Bovine osteochondral explants, concentrically incised to create chondral fissures, were maintained under cLIUS (14 kPa (5 MHz, 2.5 Vpp), 20 min, 4 times/day) for a period of 28 days in the presence or absence of cytokines, interleukin-6 (IL-6) or tumor necrosis factor (TNF)α. Outcome assessments included histological and immunohistochemical staining of the explants; and the expression of …

A Bioeconomic Model For The Optimization Of Local Canine Rabies Control, Aaron Anderson, Johann Kotze, Stephanie A. Shwiff, Brody Hatch, Chris Slootmaker, Anne Conan, Darryn Knobel, Louis H. Nel

United States Department of Agriculture Wildlife Services: Staff Publications

We present a new modeling tool that can be used to maximize the impact of canine rabies management resources that are available at the local level. The model is accessible through a web-based interface that allows for flexibility in the management strategies that can be investigated. Rabies vaccination, sterilization, chemo-contraception, and euthanasia can be specified and limited to specific demographic groups. Additionally, we allowed for considerable complexity in the specification of management costs. In many areas, the costs of contacting additional dogs increases as management effort increases, and this can have important strategic implications. We illustrated the application of the …

Evaluation Of A Nutraceutical Flex Choice For Anti-Arthritic Efficacy And Safety In Moderately Osteoarthritic Dogs, Rachael E. Webber

Murray State Theses and Dissertations

Arthritis is a very common chronic debilitating disease in people and dogs alike. It does not discriminate based on species, and one in five dogs suffer from osteoarthritis. The purpose of this study was to evaluate the safety and anti-arthritic efficacy of a nutraceutical, Flex Choice, in moderately arthritic canines. Five moderately arthritic canines that weighed between 40-65 pounds were selected for this study. Moderate arthritis was considered a ranking of 4-6 on a scale of 1-10 with 1 being no/minimal pain and 10 being severe and constant pain. All five canines were orally administered one Flex Choice soft chew …

Single Fixed-Time Artificial Insemination With Fresh Or Frozen Thawed Semen In Pig, Pachara Pearodwong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Single fixed-time AI is the breeding protocol inseminating the sows once with either fresh or frozen-thawed (FT) semen at fixed time after ovulation induction without estrus detection. To develop the success single fixed-time AI protocol in Thailand, factors affecting the efficacy of single fixed-time AI were evaluated. Additionally, we also evaluated the effect of using lecithin in cryopreservation extender on FT semen qualities in order to find the possibility to perform single fixed-time AI using sperm preserved in lecithin based extender. It was found that administration of 10 ug buserelin at 72 h after weaning decreased weaning-to-ovulation interval compared to …

Short Term Effects Of Sildenafil In Treatment Of Pulmonary Hypertension In Degenerative Mitral Valve Disease Dogs, Karun Saetang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Degenerative mitral valve disease (DMVD) is the most common acquired cardiac disease in small breed dogs. Pulmonary hypertension (PH) is a common complication in DMVD that can worsen the clinical signs, cardiac function, and median survival time of DMVD dogs. Conventional therapy of DMVD can reduce systolic pulmonary arterial pressure (sPAP) minimally. Thus, additional therapy is required. Sildenafil is used to treatment of PH in various causes, but the effect of sildenafil in the treatment of PH secondary to DMVD is not published yet. Therefore, this study aimed to evaluate the effects of sildenafil in combination with conventional therapy in …

The Invention Of Plastic Plate For The Semiquantitative Detection Of Somatic Cells In Farm Bulk Tank Milk, Piyarat Jiarpinitnun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Bulk tank milk somatic cell count (BTM SCC) is worldwide acceptable as an indicator of subclinical mastitis and milk quality at the farm level. Several countries determine the BTM SCC level for quality control and milk pricing. In Thailand, BTM SCC standard is set at 500,000 cells/ml and premium milk quality is set at less than 100,000 cells/ml. Milk collecting centers (MCCs) use CMT (California Mastitis Test) for screening raw milk quality. However, CMT has an error and high variation. This study aims to invent a new semiquantitative device for determining BTM SCC and to validate the device in comparison …

Assessment Of Left Atrial Function In Feline Hypertrophic Cardiomyopathy By Using Two Dimensional Speckle Tracking Echocardiography, Arisara Kiatsilapanan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hypertrophic cardiomyopathy (HCM) is one of the most common myocardial diseases in cats. The left atrial (LA) function plays an essential role in compensatory mechanism of left ventricle with diastolic dysfunction to maintain cardiac output in cats affected with HCM. Nowadays, there are several echocardiographic techniques used to evaluate the left atrial function; however, each technique has limitations. To overcome the limitations, two-dimensional speckle tracking echocardiography (2D-STE) is a novel technique for assessment the LA function. To date, no study focusing on assessment of the left atrial function by 2D-STE in feline HCM has been reported. The objective of this …

Vector Competence Of Culex Tritaeniorhynchus For Duck Tembusu Virus, Jitra Sanisuriwong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Duck Tembusu virus (DTMUV), an emerging infectious disease in ducks, belongs to the Ntaya virus group of the Flavivirus genus in the Flaviviridae family. The emergence of DTMUV has been observed in layer and breeder duck farms in China since 2010 and in layer and broiler duck farms in Thailand since 2013. Infected ducks show neurologic signs, including an incapability to stand, ataxia, and paralysis. A significant drop in egg production is usually observed among layer ducks. The transmission of DTUMV involves mosquito vectors, however, the exact role of mosquitoes in the ecology of DTMUV in Thailand remains unclear. The …

Development Of Mucoadhesive Nanovaccine Against Columnaris Disease In Red Tilapia (Oreochromis Sp.), Sirikorn Kitiyodom

Chulalongkorn University Theses and Dissertations (Chula ETD)

Tilapia (Oreochromis sp.) is very important and high production of freshwater fish in Thailand. Columnaris disease has been now recognized as one of the most serious infectious diseases in farmed tilapia. The disease is caused by Flavobacterium columnare. Among the prevention and control strategies, vaccination is one of the most effective approach. According to the pathogenesis of this bacteria, the characteristic lesion is almost at mucosal area of skin and gill. Therefore, hypothesized that the mucosal nanovaccine with mucoadhesive characteristic could be suitable vaccination method to control columnaris disease. In this study, we determined vaccine strain candidate by clinical field …

Epidemiological Surveillance Of Feline Morbillivirus Infection In The Aspects Of Polymerase Chain Reaction, Indirect Elisa And Immunohistochemistry, Surangkanang Chaiyasak

Chulalongkorn University Theses and Dissertations (Chula ETD)

Feline morbillivirus (FeMV), a relatively new virus, has been discovered in domestic cats and associated with kidney diseases since 2012 in Hong Kong. The FeMV study gained widely attentions in many countries whereas the pathogenesis remains elusive. These studies aimed to provide the FeMV knowledge in Thai domestic cat by performing the genetic-based, serological-based and pathological-based studies of FeMV-infected in Thai cats. Additionally, the indirect enzyme linked immunosorbent assay (ELISA) and rabbit polyclonal antibody against FeMV matrix (FeMV-M) protein was produced for seroprevalence and viral localization by immunohistochemistry (IHC) techniques, respectively. Our results revealed the FeMV RNA prevalence at 11.9% …

Evidence Of Vertical And Horizontal Transmission Of Francisella Noatunensis Subsp. Orientalis In Hybrid Red Tilapia (Orerochromis Sp.), Vuong Viet Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Francisellosis caused by Franciscella orientalis (formerly named: Franciscella noatunensis subspecies orientalis) has been reported as one of the most important emergent diseases of warm water fish. However, little is known about its transmission, fastidious nature, survival, and persistence in multiple environments and global presence. This study aims to investigate vertical transmission, horizontal transmission by vectors and effect of other organisms on the outbreak of francisellosis in hybrid red tilapia. To prove the transmission of F. orientalis from subclinically infected tilapia mouthbrooders to their offspring through the current practice of fry production in tilapia hatcheries, experimentally infected hybrid red tilapia broodstock …