Veterinary Medicine Commons^™

The Effect Of Infection Order Of Porcine Circovirus Type 2 And Porcine Reproductive And Respiratory Syndrome Virus On Dually Infected Swine Alveolar Macrophages, Yi-Chieh Tsai, Hui-Wen Chang, Chian-Ren Jeng, Tsang L. Lin, Chun-Ming Lin, Cho-Hua Wan, Victor Fei Pang

Department of Comparative Pathobiology Faculty Publications

Abstract Background: Concurrent infection with porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) is known as one of the major causes for porcine respiratory disease complex (PRDC). Dual infection with PCV2 and PRRSV is consistently to have more severe clinical presentations and pulmonary lesions than infection with PCV2 alone or PRRSV alone. However, it is not known if dual infections with PCV2 and PRRSV in different infection order may lead to different clinical symptoms in the host. To mimic the possible field conditions, swine alveolar macrophages (AMs) were inoculated with PCV2 and PRRSV in vitro …

Ultraviolet Radiation–Induced Cataract In Mice: The Effect Of Age And The Potential Biochemical Mechanism, Jie Zhang, Hong Yan, Stefan Lofgren, Xiaoli Tian, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

PURPOSE. To study the effect of age on the morphologic and biochemical alterations induced by in vivo exposure of ultraviolet radiation (UV).

METHODS. Young and old C57BL/6 mice were exposed to broadband UVBþUVA and euthanized after 2 days. Another batch of UV-exposed young mice was monitored for changes after 1, 2, 4, and 8 days. Age-matched nonexposed mice served as controls. Lens changes were documented in vivo by slit-lamp biomicroscopy and dark field microscopy photographs ex vivo. Lens homogenates were analyzed for glutathione (GSH) level, and the activities of thioredoxin (Trx), thioltransferase (TTase), and glyceraldehyde-3-phosphate dehydrogenase (G3PD). Glutathionylated lens proteins …

Investigation Of The Role Of Tnf-A Converting Enzyme (Tace) In The Inhibition Of Cell Surface And Soluble Tnf-A Production By Acute Ethanol Exposure., Kristine Von Maltzan, Wei Tan, Stephen B. Pruett

CVM Publications

Toll-like receptors (TLRs) play a fundamental role in the immune system by detecting pathogen associated molecular patterns (PAMPs) to sense host infection. Ethanol at doses relevant for humans inhibits the pathogen induced cytokine response mediated through TLRs. The current study was designed to investigate the mechanisms of this effect by determining whether ethanol inhibits TLR3 and TLR4 mediated TNF-? secretion through inhibition of transcription factor activation or post-transcriptional effects. In NF-?B reporter mice, activation of NF-?B in vivo by LPS was inhibited by ethanol (LPS alone yielded 170,000?35,300 arbitrary units of light emission; LPS plus ethanol yielded 56,120?16880, p = …

Variation In Genes Related To Cochlear Biology Is Strongly Associated With Adult-Onset Deafness In Border Collies, Jennifer S. Yokoyama, Ernst T. Lam, Alison L. Ruhe, Carolyn A. Erdman, Kathryn R. Robertson, Aubrey A. Webb, D. Colette Williams, Melanie L. Chang, Marjo K. Hytönen, Hannes Lohi, Steven P. Hamilton, Mark W. Neff

Anthropology Faculty Publications and Presentations

The domestic dog offers a unique opportunity to study complex disorders similar to those seen in humans, but within the context of the much simpler genetic backgrounds of pure breeds, which represent closed populations. We performed a whole-genome search for genetic risk factors of adult-onset deafness in the Border Collie, a breed of herding dog that relies on acute hearing to perceive and respond to commands while working. Adult-onset deafness in Border Collies typically begins in early adulthood and is similar to age-related hearing loss in humans. This earlier onset has particular impact on the utility of working Border Collies …

Canine Urinary Incontinence Post-Neutering: A Review Of Associated Factors, Pathophysiology And Treatment Options, Suppawiwat Ponglowhapan, Muhammad Khalid, David Church

The Thai Journal of Veterinary Medicine

Canine urinary incontinence is commonly encountered in small animal veterinary practice with highprevalence in spayed female dogs. The diagnosis of neutering-induced urinary incontinence is usually based onclinical signs, history and elimination of other possible diagnoses. The proposed predispositions to incontinence thatfollows neutering including gender, breed, body weight, obesity, tail docking, spaying technique (ovariectomy andovariohysterectomy) and morphology of the LUT, e.g. the position of neck of the bladder and urethral length, have allbeen investigated. At present, the exact underlying mechanisms are not fully understood. However, it is widelyagreed that the condition is multi-factorial and hormone-associated. The role of gonadotrophin LH and …

Preliminary Study Of The Clinical Outcome Of Using Pcso-524 Polyunsaturated Fatty Acid Compound In The Treatment Of Canine Osteoarthritis And Degenerative Spinal Diseases, Natwadee Mongkon, Kumpanart Soontornvipart

The Thai Journal of Veterinary Medicine

The clinical outcome of PCSO-524 application in the treatment of canine osteoarthritis and spinal disorderwas studied at the Small Animal Teaching Hospital, Chulalongkorn University. Eighty-four dogs were included inour study. Thirty-one dogs showed signs of osteoarthritis (OA) in both hip joints and shoulder joints; 33 dogs had OAin stifle joints from cranial cruciate ligament rupture; and 20 dogs had neurological signs of the cauda equinasyndrome. All affected dogs were treated by 50 mg/ 10 kg of PCSO-524, PO for twelve weeks. Neurological grading,lameness scores and neurological examinations were evaluated before and after the administration of PCSO-524 fortwo and four weeks. …

Experimental Infection Of Mice And Baby Chickens With Thailand Strain Of Chikungunya Virus, Sonthaya Tiawsirisup, Pichanan Rattanakampol, Warritar Navavichit, Hemmarat Ratpiyapaporn

The Thai Journal of Veterinary Medicine

This study was conducted to investigate Chikungunya virus (CHIKV) infection in mammal and avian byusing mice and baby chickens as model animals. Thailand 2010 and Ross/186 strain (reference strain) were used inthis study. 104, 106 and 108 CID50 of CHIKV were inoculated to four- and six-week-old mice. Blood was collected andtested for virus for seven days. 108 CID50 of CHIKV was inoculated to two-week-old mice. Blood was collected andtested for virus for five days. For the baby chickens, 102, 104, 106 and 108 CID50 of CHIKV were inoculated to five-dayoldbaby chickens. Blood was collected and tested for virus for seven …

Distribution Of Spermatozoa In The Reproductive Tracts Of Sows After Intra-Uterine Insemination Using Frozen-Thawed Boar Semen, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The aims of the present study were to determine the number of spermatozoa in the reproductive tract ofsows after intra-uterine insemination (IUI) with frozen-thawed (FT) boar semen and to investigate the influence ofadding seminal plasma in the thawing medium on the sperm transport in the female reproductive tract. Fourteenmultiparous sows were divided into 3 groups: group I (n= 5), insemination with 80 ml of extended fresh semen,group II (n= 4), insemination with 40 ml of FT semen diluted with ModenaTM extender and group III (n= 5),insemination with 40 ml of FT semen diluted with combination of ModenaTM and seminal plasma. …

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

Hand, Foot, And Mouth Disease, Juthatip Keawcharoen

The Thai Journal of Veterinary Medicine

No abstract provided.

Anxiolytic-Like Effects Of Noni Juice (Morinda Citrifolia L.) On The Respective Changes Of Neurotransmitters In Rat Brain In The Elevated Plus-Maze Test, Sarinee Kalandakanond-Thongsong, Jantarima Charoenphandhu

The Thai Journal of Veterinary Medicine

Noni (Morinda citrifolia L.) has long been used to treat various conditions in folklore medicine. Presently,most studies focus on antioxidant and anti-cancer activities while it is also claimed by noni juice-consumers toproduce happiness. Mood disorders, e.g. anxiety, are symptoms that reflected the disorder of neurotransmitters, oneof the most prominent is the monoaminergic system. Anxiety affects people throughout the world; people have beenseeking medicine for treatment while natural products may also alleviate this unfavorable symptom. The presentstudy examined whether noni contained anxiolytic-like effect when tested with elevated plus-maze (EPM), a standardtest for anxiety, and whether this effect was related to change …

Side Effects In 412 Dogs From Swimming In A Chlorinated Swimming Pool, Korakot Nganvongpanit, Terdsak Yano

The Thai Journal of Veterinary Medicine

This study reports the side effects in dogs from swimming in a chlorinated swimming pool. Data werecollected from September 2008 until April 2012. A total of 412 dogs (male= 219 and female= 193), aged 38±30 months,served as the subjects. The dogs were divided into four groups based on the aims of swimming: entertainment (n=152), weight reduction (n= 53), rehabilitation after orthopedic surgery (n= 81), and rehabilitation for muscle and jointdisease (n= 126). The data were recorded for five separate swimming times. At first, some dogs (29.13%) showedoverexcitement and/or fear, but this percentage decreased with increased swimming frequency. Some dogs (36.51%)were …

An Anatomical, Histological And Ultrastructural Study On The Placenta Of The Red-Shanked Douc Langur (Pygathrix Nemaeus), Damri Darawiroj, Natthaya Tuaprakone, Yongchai Utara

The Thai Journal of Veterinary Medicine

A red-shanked douc langur (Pygathrix nemaeus nemaeus) is currently enlisted as an endangered primatespecies. This study was undertaken to describe the gross anatomy, histology and ultrastructural morphology of thedouc langur term placenta by light, scanning, and transmission electron microscopy. The primary disc of bidiscoidalplacenta was separated to 4-6 small cotyledonary subdivisions and was attached by an umbilical cord. The chorionfrondosum of placenta was classified as villous, haemomonochorial and deciduate type. The interhaemal area wascomposed of a layer of syncytiotrophoblast that occasionally clustered as the syncytial knot, fetal capillaryendothelium and its basement membrane. The intervillous mineralized fibrinoid mass and syncytial knot …

Detection Of Enzootic Bovine Leukosis In Cattle Using Nested Polymerase Chain Reaction Assay, Jomelson Alfaro Uera, Jonathan Ventura Lazaro, Claro Niegos Mingala

The Thai Journal of Veterinary Medicine

Enzootic bovine leukosis (EBL) is caused by bovine leukemia virus (BLV) infection. BLV was detected incattle using nested polymerase chain reaction (PCR) assay and were identified BLV infected cattle farms in fiveselected provinces in the Philippines. A total of 300 cattle blood samples were used. BLV Proviral DNA wasextracted and amplified using nested PCR assay targeting the BLV long terminal repeat (LTR). Results showed that11 samples (3.67%) of the 300 cattle blood samples used were positive for BLV infection. This study is considered firstreport of cattle EBL in the Philippines.

Molecular Analysis Of Medically And Veterinary Important Muscid Flies (Diptera: Muscidae) In Thailand, Payu Bhakdeenuan, Padet Siriyasatien, Sunchai Payungporn, Kanok Preativatanyou, Usavadee Thavara, Apiwat Tawatsin, Kom Sukontason, Kabkaew Likitvong Sukontason, Wej Choochote, Suttida Suwannayod, Hitoshi Sasaki

The Thai Journal of Veterinary Medicine

We demonstrated the using of the internal transcribed spacer (ITS2) of ribosomal DNA as a tool for identification of medically and veterinary important Muscidae flies in Thailand. A total of 27 fly samples were collected from various regions of Thailand. Six fly species in three subfamilies including Azeliinae (Hydrotaea spinigera), Muscinae (Musca domestica, M. sorbens) and Stomoxyinae (Stomoxys calcitrans, S. indicus and S. sitiens) were identified base on morphological taxonomy. PCR amplicons of the ITS2 gene of these flies varied between 312-377 bp with A+T content of 76.6%. ITS2 sequences of the flies in this study were 93-100% identity to …

Surgical Treatment Of Renal Gout In Monocellate Cobra, Naja Kaouthia, Taksa Vasaruchapong, Lawan Chanhome

The Thai Journal of Veterinary Medicine

A wild captured, male Monocellate cobra, Naja kaouthia of unknown age was observed with anorexia, imbalance of swimming and mild swelling in the distal fourth of the body. Physical examination revealed a palpable firm mass in the coelomic cavity, of which radiographic examination was suspicious as an obstruction of large intestine by a foreign body. Removal of the foreign body through its cloaca was attempted by enema and digital manipulation, but was not successful. Thereafter, an exploratory surgery was performed and revealed an abnormality in the left kidney that was compressing the large intestine. Nephrectomy was performed and histopathologically identified …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Influence Of Intracellular Glutamine Depletion On Regulation Of Amino Acid Transport System A In Placental (Bewo) Cells, Boonrit Thongsong

The Thai Journal of Veterinary Medicine

Glutamine is known to modulate the expression of various genes and uptake activity of nutrient transportersin mammalian cells. The purpose of the study was to investigate the influence of intracellular glutamine status onamino acid transport system A in placental choriocarcinoma cells (BeWo). Intracellular glutamine was depleted byculturing the cells in regular medium without glutamine and by treating the cells for 16 hours with methioninesulfoximine (MSX), an inhibitor of glutamine synthetase. When cultured in the presence of glutamine, treatment withMSX had no effect on system A activity as monitored by MeAIB uptake. However, when cultured in the absence ofglutamine, treatment with …

Differential Expression Patterns Of Proteins Involved In Epidermal Proliferation And Differentiation In Canine Atopic Dermatitis, Sirin Theerawatanasirikul, Achariya Sailasuta, Roongroje Thanawongnuwech, Tossaporn Nakbed, Komkrid Charngkaew, Gunnaporn Suriyaphol

The Thai Journal of Veterinary Medicine

The pathological alterations in skin keratinocyte proliferation and differentiation in canine atopic dermatitis(CAD) were investigated in 20 small breed dogs with CAD (10 of which displayed lesional CAD skin and 10 nonlesionalCAD skin) and 11 healthy control animals. Biopsy of lesional CAD skin showed orthokeratotichyperkeratosis. Immunohistochemistry (IHC) of Ki-67, a cellular marker for proliferation, showed a higher epidermalcell proliferation rate in the CAD dogs (p< 0.05) which positively correlated with Canine Atopic Dermatitis Extent andSeverity Index, CADESI-03 (p< 0.01). IHC labeling indicated reduced expression of cornified envelope proteins,involucrin (IVL) and filaggrin (FLG) in both lesional and non-lesional skin (p< 0.05). In contrast, expression oflympho-epithelial Kazal-type inhibitor (LEKTI), which has been reported to inhibit proteases that cleave proFLG intoFLG, was increased in lesional skin compared to the normal controls (p< 0.05). The IHC results showed a positivecorrelation between CADESI-03 and LEKTI expression and a negative correlation between CADESI-03 and IVLexpression. In conclusion, the present study demonstrates that epidermal hyperkeratosis in CAD is related todisturbance of both epidermal proliferation and cornified envelope differentiation. This is the first report of acorrelation of LEKTI with CAD.

Toxicity Test Of Nanosilver Particles On Zebrafish (Danio Rerio) Embryonic Development, Theerayuth Kaewamatawong, Aranya Ponpornpisit, Wijit Banlunara, Anong Bintvihok, Benchawan Tosukcharoen, Siriwipa Kongloon, Suthasinee Udchachon, Pattwat Maneewattanapinyo, Chuchaat Thammacharoen, Sanong Ekgasit

The Thai Journal of Veterinary Medicine

Contamination of the environment with Nanosilver particles (AgNPs) has caused considerable concern. Atpresent there is little information of AgNPs toxicity, especially in aquatic environments. The purpose of this studywas to evaluate toxic effects of AgNPs on Zebrafish (Danio rerio) embryonic development. Toxicity test of AgNPs onthe embryos was conducted in concentration of 0.05, 0.1, 0.5, 1 and 5 μg/ml. Standard water was used as control.Observation for mortality, morphological and functional abnormalities at 4, 24, 48 and 72 hours post fertilization (hpf)were performed. The results showed that AgNPs at 1 and 5 μg/ml had significant lethal effect on the embryos.Calculated LC50 …

Stimulation Patterns For Erection And Ejaculation Using Electroejaculator In Black Marsh Turtles, Sakdichod Kimskulvech, Peerasak Suttiyotin

The Thai Journal of Veterinary Medicine

Semen collection by an electroejaculator was studied in black marsh turtles. High responses were recordedwhen electrical probe was inserted to the depth of 5.9±0.1 cm from the anus or 5.2±0.2 cm from posterior tip of thecarapace. The depth position was approximately the center of the second carapace from the rear. The intensity ofresponses increased with increasing voltage and reached a plateau at 7.0 volts. The depth of the probe inserted toyield highest responses had higher relationship with carapace length when measured against the anus (r= 0.81, p=0.0088) than the posterior end of carapace (r= 0.06, p= 0.8825). Stimulation voltages have …

Safety Evaluations Of Ethanolic Extract Of Moringa Oleifera Lam. Seed In Experimental Animals, Songpol Chivapat, Pornchai Sincharoenpokai, Praw Suppajariyawat, Anudep Rungsipipat, Suphan Phattarapornchaiwat, Vanida Chantarateptawan

The Thai Journal of Veterinary Medicine

The safety of ethanolic extract from the seeds of Moringa oleifera was evaluated for its oral toxicity by acute toxicity test and subchronic toxicity study in experimental animals. The acute toxicity test in mice showed that the extract at doses ranging from 5.1 to 10.0 g/kg caused toxic signs and dose dependent mortality. Oral administration of the extract in rats at doses of 100, 500 and 1000 mg/kg/day for 90 consecutive days revealed that male rats treated with the extract at 1000 mg/kg had a significant decrease in RBC but this alteration was within rats reference range. A significant decrease …

Horner's Syndrome Associated With Escherichia Coli Infection In A Raccoon (Procyon Lotor) - A Case Report, Fernando Nájera Muñoz, Alfredo Carlos Suárez Saavedra

The Thai Journal of Veterinary Medicine

This clinical case describes a three-year-old male raccoon (Procyon lotor), castrated, from La Lajita Oasis ParkZoological Park (Spain) which showed lack of appetite, state of mental depression and mild dehydration. Duringclinical examination an abscess was detected in the left ventrolateral area of the neck as well as clinical signscompatible with Horner’s Syndrome in the left eye. A hypersensitivity test was carried out by sympatheticinnervation with topical epinephrine 0.0001% and results suggested the presence of second-order Horner’s Syndromeformed by pressure from the abscess on the sympathetic innervation of the left eye. A radiologic study of the neckwas performed and blood and …

Apitoxin Aquapuncture And Administration Of Herbal Medicines In A Dog With Superficial Pyoderma, Cheng-Hung Lai, Pan-Chen Liu, Han-Yuan Lin

The Thai Journal of Veterinary Medicine

A 6-month-old, 7.2 kg, intact female mongrel stray dog wasreferred to the Veterinary Medical TeachingHospital, National Chung Hsing University with a chief complaint of alopecia, and severe pruritus. A skin swab wasobtained for bacterial culture, and Staphylococcus pseudintermedius was identified. The definitive diagnosis was caninesuperficial pyoderma. Apitoxin aquapuncture was performed to treat superficial pyoderma once a week for 5 weeks.The acupoints used in this clinical case included GV-14 (Da Zhui), BL-13 (Fei Shu), LI-11 (Qu Chi), BL-40 (WeiZhong), ST-36 (Zu San Li), SP-6 (San Yin Jiao), and SP-10 (Xue Hai). In addition, the herbal medicines Shi Wei Bai DuTang, Huang …

Preservation Of Sudan Red Staining Of Brain After Plastination Process, Punnarat Vibulchan, Jantima Intarapanya, Ornsiri Cheunsuang

The Thai Journal of Veterinary Medicine

Specimen preservation is an essential factor in gross anatomical study of the brain and spinal cord. Forcedimpregnation of the specimen using polymer, or plastination, is to date one of the best specimen preparationprocedures. This study aimed to study the possibility of staining whole brain specimen using Sudan Red III dye priorto plastination. Dog brains were divided into 3 groups each stained with the dye for 1, 2 and 3 months, respectively.The stained specimens were then dissected to reveal the staining of deep structures before undergoing theplastination procedure. After the experiment we found that Sudan Red clearly stained the fiber tracts …

Combination Of Acupuncture And Aquapuncture Using Vitamin B Complex For Treatment Of Chronic Degenerative Changes Of Hip Joints And Anal Relaxation In A Dog, Kui-Ming Chang, Kuan-Sheng Chen, Hsien-Chi Wang, Cheng-Hung Lai, Yu-Ta Hsieh, Hsing-Hui King, Wei-Ming Lee

The Thai Journal of Veterinary Medicine

A beagle dog, aged 10 years old, was presented with anal relaxation. Chronic arthrosis of hip joints had beendiagnosed and treated on this dog’s first visit to the National Chung Hsing University Veterinary Medicine Teachinghospital. Except for unwillingness to walk on both hind limbs, pain on both hips and uncontrolled defecation due toanal relaxation, no other clinical signs were found during physical examination. The body temperature was 38.60C.CRT was normal (less than 2 sec). Heart beat was 110 beats/min and strong pulse was palpated. Based on theGlasgow composite pain scale, the pain scale of this dog was 2. Radiology of …

Ecg Quiz, Chollada Buranakarl, Suwanakiat Sawangkoon, Monkon Trisiriroj, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.

Mutations In Dmrt3 Affect Locomotion In Horses And Spinal Circuit Function In Mice, Lisa S. Andersson, Martin Larhammar, Fatima Memic, Hanna Wootz, Doreen Schwochow, Carl-Johan Rubin, Kalicharan Patra, Thorvaldur Arnason, Lisbeth Wellbring, Göran Hjälm, Freyja Imsland, Jessica Lynn Petersen, Molly E. Mccue, James R. Mickelson, Gus Cothran, Nadav Ahituv, Lars Roepstorff, Sofia Mikko, Anna Vallstedt, Gabriella Lindgren, Leif Andersson, Klas Kullander

Department of Animal Science: Faculty Publications

Locomotion in mammals relies on a central pattern-generating circuitry of spinal interneurons established during development that coordinates limb movement. These networks produce left–right alternation of limbs as well as coordinated activation of flexor and extensor muscles. Here we show that a premature stop codon in the DMRT3 gene has a major effect on the pattern of locomotion in horses. The mutation is permissive for the ability to perform alternate gaits and has a favorable effect on harness racing performance. Examination of wild-type and Dmrt3-null mice demonstrates that Dmrt3 is expressed in the dI6 subdivision of spinal cord neurons, takes …

Development Of Repressible Systems To Control Gene Expression In Vaccinia Virus, Allison Titong

Master's Theses

Two vaccinia virus (VACV) expression systems that contain elements from the lactose (lac) and the tetracycline (tet) operons of E. coli were developed to repress the expression of a reporter gene, enhanced green fluorescent protein (EGFP), in the presence of tet operon inducers. In the first system, lac and tet operon elements were arranged in a gene circuit, and in the presence of increasing concentrations of a lac operon inducer (isopropyl-β-D-thiogalactoside, IPTG), EGFP expression increased in a dose dependent manner and at high IPTG concentrations, expression reached the same levels as a positive control virus. Importantly, in the …