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2012

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Articles 151 - 180 of 271

Full-Text Articles in Veterinary Medicine

Breeding Induced Endometritis In The Mare: The Local Innate Immune Response, Elizabeth Moran Woodward Jan 2012

Breeding Induced Endometritis In The Mare: The Local Innate Immune Response, Elizabeth Moran Woodward

Theses and Dissertations--Veterinary Science

Uterine inflammation after breeding is considered necessary for the clearance of excess semen and debris from the uterus. A subpopulation of mares fails to clear the inflammation in a timely fashion, and develops a persistent breeding induced endometritis (PBIE). Experiments were preformed to evaluate correlations of PBIE to endometrial quality and age. Mares of advanced age and poor endometrial quality had a higher incidence of PBIE. In addition, mares fluctuated in susceptibility to PBIE from one season to the next. The uterine inflammatory gene expression in susceptible and resistant mares within the first 24 hours after breeding was investigated. The …

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Development Of Molecular Diagnostic Assays For Equine Respiratory Viruses And Analysis Of The Role Of Equine Arteritis Virus Envelope Proteins In The Early Events Of Virus Entry, Zhengchun Lu Jan 2012

Development Of Molecular Diagnostic Assays For Equine Respiratory Viruses And Analysis Of The Role Of Equine Arteritis Virus Envelope Proteins In The Early Events Of Virus Entry, Zhengchun Lu

Theses and Dissertations--Veterinary Science

There is an urgent need for detection of viral respiratory pathogens to identify the causal agent(s) involved and to prevent the spread of related diseases. The first part of this dissertation focuses on development, optimization and validation of Real-time reverse transcription polymerase chain reaction (rRT-PCR) assays for the detection of several common equine viral pathogens: equine arteritis virus (EAV), equine influenza virus and equine rhinitis viruses A and B. Emphasis of the second part of this dissertation is on studying the role of EAV envelope proteins in virus attachment and entry. Using an infectious cDNA clone of EAV and reverse …

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Role Of Intraocular Leptospira Infections In The Pathogenesis Of Equine Recurrent Uveitis In The Southern United States, Florence Polle Jan 2012

Role Of Intraocular Leptospira Infections In The Pathogenesis Of Equine Recurrent Uveitis In The Southern United States, Florence Polle

LSU Master's Theses

To investigate the role of intraocular leptospiral infections in horses with Equine Recurrent Uveitis (ERU), ocular fluid samples were collected from donated and client-owned horses with a history and ocular findings consistent with chronic ERU. Additionally, eyes were harvested from horses with normal ophthalmic examinations as a control group. Blood samples were obtained for Leptospira serology using microscopic agglutination test (MAT). Aqueous and vitreous humor samples were aseptically obtained and submitted for aerobic culture and Leptospira culture, PCR and MAT. Twenty-one control horses (40 eyes) and 31 ERU horses (46 eyes) were available for study. Serology results were available for …

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Isolation And Characterization Of Adult Progenitor Cells From Healthy And Laminitic Hoof Tissue, Vanessa Pinto Jan 2012

Isolation And Characterization Of Adult Progenitor Cells From Healthy And Laminitic Hoof Tissue, Vanessa Pinto

LSU Master's Theses

Laminitis is an often fatal condition in horses with few available and only moderately effective treatment options. Separation of laminar dermis and epidermis lead to rotation or ventral deviation of the third phalanx inside the hoof capsule. Despite being a modified skin, equine laminar tissue does not completely return to normal after laminitis. The hypothesis tested was that adult progenitor cells in the equine laminar tissue are irreversibly damaged by laminitis. A method to harvest and culture cells in vitro from the equine lamina was established; and progenitor cells from unaffected and laminitic hooves were characterized and compared. Laminar tissue …

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The Role Of Gylcoprotein K (Gk) In The Ocular And Neuropathogenesis Of Herpes Simplex Virus-Type 1 (Hsv-1), Andrew Timothy David Jan 2012

The Role Of Gylcoprotein K (Gk) In The Ocular And Neuropathogenesis Of Herpes Simplex Virus-Type 1 (Hsv-1), Andrew Timothy David

LSU Doctoral Dissertations

Herpes simplex virus type 1 (HSV-1) is a DNA virus that is a ubiquitous pathogen of humans. The hallmark of the HSV-lifecycle is infection of a mucosal surface with spread to sensory neurons where the virus establishes a latent infection with periodic recurrences for the life of the host. The most common course of symptomatic disease with HSV-1 is the typical mucocutaneous lesion that is self-limiting. HSV-1 can also cause acute encephalitis and ocular pathology on reactivation. Both of these manifestations of the disease have severe consequences. Although ocular infection is less frequent, the extensive prevalence of HSV makes it …

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Morphological Characterization Of The Immune Organs In Ostrich Chicks, Hui Song, Ke-Mei Peng, Sheng-He Li, Yan Wang, Lan Wei, Li Tang Jan 2012

Morphological Characterization Of The Immune Organs In Ostrich Chicks, Hui Song, Ke-Mei Peng, Sheng-He Li, Yan Wang, Lan Wei, Li Tang

Turkish Journal of Veterinary & Animal Sciences

This study describes the anatomical, light microscopic, and ultrastructural characterization of the immune organs in ostrich chicks. In this study 6 healthy 8-week-old ostrich chicks were processed for light and transmission electron microscopy. Hematoxylin and eosin as well as improved Weigert staining were used for light microscopy. The small thymus, which clustered caudally along both sides of the neck, contained Hassall's corpuscles in the medulla. The cloacal bursa, which formed the hanging and lateral walls of the cloaca, enveloped the cloaca but did not form a truly independent bursa. Inside the bursa convex papillae were densely distributed on the surface …

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Full Length Cdna Synthesis Of Differentially Displayed Ests During Lactation In The Indian Buffalo (Bubalus Bubalis), Ajai Tripathi, Krishna M. Singh, Makrand N. Vaze, Umed V. Ramani, Dharamsi N. Rank, Chaitanya G. Joshi Jan 2012

Full Length Cdna Synthesis Of Differentially Displayed Ests During Lactation In The Indian Buffalo (Bubalus Bubalis), Ajai Tripathi, Krishna M. Singh, Makrand N. Vaze, Umed V. Ramani, Dharamsi N. Rank, Chaitanya G. Joshi

Turkish Journal of Veterinary & Animal Sciences

In the present study, antisense primers were designed from 7 differentially displayed expressed sequence tags (ESTs) previously identified at our laboratory. Total RNA was isolated from the mammary gland tissue of lactating buffalo to carry out 5' rapid amplification cDNA ends (RACE) PCR. 5' RACE PCR amplicons, ranging from 161 bp to 336 bp, were cloned and sequenced. These 5' RACE sequences were analyzed using the blastn (Megablast) bioinformatics tool available from NCBI. Of these 7, only one 5' mRNA sequence did not show a match in the available database and another 1 failed to show a match with any …

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A Retrospective Study Of Anaplasma In Minnesota Cattle, Aneela Durrani, Sagar Goyal Jan 2012

A Retrospective Study Of Anaplasma In Minnesota Cattle, Aneela Durrani, Sagar Goyal

Turkish Journal of Veterinary & Animal Sciences

To determine the seroprevalence of Anaplasma in Minnesota cattle, the database of the Veterinary Diagnostic Laboratory (VDL), University of Minnesota was searched over a 10 year period (2001 to July 2010). A total of 438,407 bovine serum samples were tested by complement fixation test (CFT) and competitive enzyme linked immunosorbent test (cELISA). The positive rate of seroprevalence by CFT was 2.4% out of 211,484 samples, with a 0.13% anticomplementary result. The VDL at the University of Minnesota started using cELISA after 2002, and CFT application was suspended as diagnostic test for anaplasmosis after 2005 due to its complexity. With cELISA, …

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Effect Of Fermented Protein Feedstuffs On Pig Production Performance, Nutrient Digestibility, And Fecal Microbes, Juan Chang, Qingqiang Yin, Pengpeng Wang, Weimin Wang, Ruiyu Zuo, Qiuhong Zheng, Junxi Liu Jan 2012

Effect Of Fermented Protein Feedstuffs On Pig Production Performance, Nutrient Digestibility, And Fecal Microbes, Juan Chang, Qingqiang Yin, Pengpeng Wang, Weimin Wang, Ruiyu Zuo, Qiuhong Zheng, Junxi Liu

Turkish Journal of Veterinary & Animal Sciences

In order to determine the availability of fermented protein feedstuffs (FPFS)-such as cottonseed, blood, and feather meals—with Aspergillus oryzae, 2 phases of feeding experiments were adopted for 7 weeks. Sixty 58-day-old crossbred pigs were assigned to 6 groups, 10 pigs per group. Group 1 was the control; groups 2, 3, and 4 were supplemented with 6%-7%, 12%-14%, and 18%-21% FPFS, respectively, by replacing the same percentage of soybean meal (SBM); group 5 was supplemented with 12%-14% unfermented protein feedstuffs (UFPFS) by replacing the same percentage of SBM; group 6 was on the same base as group 3 with digestive energy …

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The Effect Of Bleeding On Peripheral Blood And Head Kidney Hematopoietic Tissue In Common Carp (Cyprinus Carpio), Elzbieta Kondera, Aneta Dmowska, Monika Rosa, Malgorzata Witeska Jan 2012

The Effect Of Bleeding On Peripheral Blood And Head Kidney Hematopoietic Tissue In Common Carp (Cyprinus Carpio), Elzbieta Kondera, Aneta Dmowska, Monika Rosa, Malgorzata Witeska

Turkish Journal of Veterinary & Animal Sciences

Adverse environmental conditions (contamination of water with nitrite, pesticides, or other pollutants), nutritional deficiencies, bacterial and viral infections, and parasitic invasions may induce anemia in fish, in both natural and aquaculture conditions. Changes in hematological values and cellular composition of peripheral blood and head kidney hematopoietic tissue of bled common carp (Cyprinus carpio L.) were evaluated over a 4 week period post-bleeding (30% blood loss). The results showed that fish very quickly compensated for anemia. Erythrocyte count (RBC), hemoglobin concentration (Hb), and hematocrit (Ht) gradually increased to values significantly higher than they were before bleeding; this was accompanied by an …

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The Effect Of Different Extenders On The Motility And Morphology Of Ram Sperm Frozen Or Stored At 4 °C, Recai̇ Kulaksiz, Çi̇ğdem Çebi̇, Ergün Akçay Jan 2012

The Effect Of Different Extenders On The Motility And Morphology Of Ram Sperm Frozen Or Stored At 4 °C, Recai̇ Kulaksiz, Çi̇ğdem Çebi̇, Ergün Akçay

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to evaluate the effects on sperm motility and abnormality in ram semen extended with skimmed milk (M), sodium citrate (SC), Tris (T), and Bioxcell® (B) after storage in liquid form at 4 °C and in frozen form. Ejaculates were collected from 3 Akkaraman rams by artificial vagina twice a week during the non-breeding season. After pooling, each pooled ejaculate was split into 4 equal aliquots and diluted with skimmed milk (M), sodium citrate (SC), Tris (T), and Bioxcell® (B) extenders. Sperm motility was significantly higher (P < 0.05) in M compared with B, SC, and T on the first and second days (48 h) of storage. With regard to the percentage of total abnormal spermatozoa, the results in M were different from (P < 0.05) from those of SC and T on the first day (24 h) of storage but no different from B. Differences among extenders were found to be significant post-thawing for spermatozoa motility and the percentage of total abnormal spermatozoa. The total abnormality of semen diluted with M was significantly lower than that observed in the other extenders. Consequently, it was found that skimmed milk was better than the other extenders in terms of the sperm parameters evaluated during liquid storage and post-thaw.

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Reproductive Performance Of Primiparous And Multiparous Saanen Goats After Laparoscopic Intrauterine Insemination: A Field Study, Recai̇ Kulaksiz, Ali̇ Daşkin Jan 2012

Reproductive Performance Of Primiparous And Multiparous Saanen Goats After Laparoscopic Intrauterine Insemination: A Field Study, Recai̇ Kulaksiz, Ali̇ Daşkin

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the reproductive performance of primiparous and multiparous Saanen goats after intrauterine laparoscopic artificial insemination with frozen semen. Twenty-four Saanen goats, divided in 2 groups: group 1 consisted of 11 primiparous goats and group 2 consisted of 13 multiparous goats. Estrus was synchronized by 20 mg fluorogestone acetate (FGA)-impregnated intravaginal sponges and the IM administration of 125 mg of cloprostenol (PGF2\alpha) and eCG (400 IU), 48 h before sponge removal. Intrauterine fixed time artificial insemination (AI) was carried out 55 h after removal of the FGA sponges. Results of the present study indicate …

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A Loop-Mediated Isothermal Amplification (Lamp) Assay For Rapid Detection Of Anaplasma Phagocytophilum Infection In Dogs, Chungchen Lee, Yungchang Lin, Chauloong Tsang, Yangtsung Chung Jan 2012

A Loop-Mediated Isothermal Amplification (Lamp) Assay For Rapid Detection Of Anaplasma Phagocytophilum Infection In Dogs, Chungchen Lee, Yungchang Lin, Chauloong Tsang, Yangtsung Chung

Turkish Journal of Veterinary & Animal Sciences

A simple and rapid diagnostic method of Anaplasma phagocytophilum infection was established, employing the loop-mediated isothermal amplification (LAMP) reaction. With 1 set of 4 specific primers targeting the citrate synthase (gltA) gene of A. phagocytophilum, the LAMP assay rapidly amplified the target DNA sequence in a single tube in 1 h, requiring only a water bath for reaction. When visualized by gel electrophoresis, the LAMP products appeared as a ladder-like pattern, with many bands of different sizes from 180 base pairs up to the loading well. The results obtained from testing 32 clinical blood samples of dogs demonstrated that the …

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Immunization Of West Nile Recombinant Envelope Domain Iii With Equine Cd40 Ligand Protein Vaccine Induced Specific Immune Response In Rabbits And Horses, Shiliang Anthony Liu Jan 2012

Immunization Of West Nile Recombinant Envelope Domain Iii With Equine Cd40 Ligand Protein Vaccine Induced Specific Immune Response In Rabbits And Horses, Shiliang Anthony Liu

LSU Master's Theses

West Nile virus (WNV) is one of several flaviruses known to infect mammalian species, including humans. There were 15,257 horse cases reported in 2002 and 1,086 in 2006 in United States. Recently, significant increases in equine and human cases have been reported in United States. Domain III of the WNV envelope protein binds to cellular receptors, and induces a significant portion of the neutralizing antibody response against the virus. CD40 Ligand (CD40L, CD154) enhances productive interactions between T cells and APC and has been shown to function as a potential adjuvant. In this study, we constructed and expressed a fusion …

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The Effects Of Iodixanol Present During Equine Semen Cryopreservation, David Paul Beehan Jan 2012

The Effects Of Iodixanol Present During Equine Semen Cryopreservation, David Paul Beehan

LSU Master's Theses

The objectives of this study were to determine what effects iodixanol would have on total and progressive motility, plasma membrane integrity (viability), acrosome integrity and DNA structure when present during cryopreservation of equine spermatozoa,. We hypothesized that the addition of iodixanol would improve post-thaw values for measured parameters. Ejaculates from six stallions were collected, centrifuged at 900 x g for ten minutes to remove supernatant, and suspended to 200 x 106 cells/ml with 0%, 2.5% and 5% iodixanol in an egg-yolk based extender and cryopreserved. Before and after cryopreservation sperm motility was assessed by computer assisted semen analysis, and samples …

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Evaluation Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Screws In Equine Third Metacarpal Bone, Myra Elizabeth Durham Jan 2012

Evaluation Of Plasma Sprayed Hydroxyapatite Coated Ao Cortical Screws In Equine Third Metacarpal Bone, Myra Elizabeth Durham

LSU Master's Theses

ABSTRACT Objectives - To compare the osteointegration of plasma sprayed hydroxyapatite (HA) coated and uncoated 5.5 mm cortical screws in equine third metacarpal (MC3) bones in combination with a 4.5mm broad dynamic compression plate (DCP). Study design - In vivo study. Animals – 6 Adult Thoroughbred horses. Methods – For each horse, four HA coated screws were placed unicortically through a 4.5mm broad DCP in the dorsal cortex of a randomly chosen MC3 bone, with 4 uncoated screws placed in an identical manner in the contralateral MC3 bone. All screws were tightened to a torque of 5.4 N-m. Extraction torques …

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Structural And Molecular Pathology Of The Atrium In Boxer Arrhythmogenic Cardiomyopathy, Jorge Luis Vila Jan 2012

Structural And Molecular Pathology Of The Atrium In Boxer Arrhythmogenic Cardiomyopathy, Jorge Luis Vila

LSU Master's Theses

Arrhythmogenic cardiomyopathy (AC), also known as arrhythmogenic right ventricular cardiomyopathy (ARVC), is a disease characterized by fatty or fibro-fatty myocardial replacement, predominantly in the right ventricle and to a lower extent the left ventricle. It is recognized as a disease affecting the cardiac intercalated disc. Clinically, it is associated with ventricular arrhythmias, although atrial arrhythmias and atrial histopathological changes characteristic of AC have occasionally been reported. The full extent of atrial involvement in AC has not been investigated. Therefore, we aimed to apply histopathology, immunochemical detection, immunolocalization and transmission electron microscopy (TEM) techniques to characterize the distribution of desmosomal and …

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Estimation Of Variance Components And Genetic Parameters For Type Traits And Milk Yield In Holstein Cattle, Serdar Duru, Salahatti̇n Kumlu, Erdoğan Tuncel Jan 2012

Estimation Of Variance Components And Genetic Parameters For Type Traits And Milk Yield In Holstein Cattle, Serdar Duru, Salahatti̇n Kumlu, Erdoğan Tuncel

Turkish Journal of Veterinary & Animal Sciences

This research was conducted to estimate variance components and genetic parameters for type traits and milk yield in Holstein-Friesian cattle. In this study, 597 daughters of 158 sires in 128 herds were classified. In the data analysis, type scores for 354 daughters bred within 70 herds sired by 46 sires that had at least 3 daughters, and 304 lactation records for 206 daughters within 56 herds sired by 37 sires, were considered. For estimation of variance components and correlations among the traits, the MTDFREML package program was used. The mean stature was 145.56 cm. Means for linear traits varied from …

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Rhodotorula Minuta Fungemia In A Ewe Lamb, C G. Chitko-Mckown, K A. Leymaster, M. P. Heaton, D D. Griffin, J K. Veatch, S A. Jones, M. L. Clawson Jan 2012

Rhodotorula Minuta Fungemia In A Ewe Lamb, C G. Chitko-Mckown, K A. Leymaster, M. P. Heaton, D D. Griffin, J K. Veatch, S A. Jones, M. L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

An 8-month-old crossbred ewe, normal upon physical examination, was humanely euthanized for tissue collection. After approximately 3 weeks in tissue culture, fungi began budding out of cells obtained from the choroid plexus. After an additional 3 weeks, budding was observed in kidney cell cultures and eventually in monocyte cultures as well. Serum from the lamb was submitted to the Veterinary Diagnostic Laboratory at Colorado State University for fungal diagnosis and was found negative for Aspergillus, Blastomyces, Coccidioidomycosis and Histoplasmosis. DNA was isolated from fungi collected from tissue culture supernatants and used in a set of pan-fungal PCR assays with DNA …

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Posttranslational Modification Of Vesicular Stomatitis Virus Glycoprotein, But Not Jnk Inhibition, Is The Antiviral Mechanism Of Sp600125, Sabrina Marozin, Jennifer Altomonte, Sibylle Apfel, Phat X. Dinh, Enrico De Toni, Antonia Rizzani, Andreas Nüssler, Nobuyuki Kato, Roland M. Schmid, Asit K. Pattnaik, Oliver Eberta Jan 2012

Posttranslational Modification Of Vesicular Stomatitis Virus Glycoprotein, But Not Jnk Inhibition, Is The Antiviral Mechanism Of Sp600125, Sabrina Marozin, Jennifer Altomonte, Sibylle Apfel, Phat X. Dinh, Enrico De Toni, Antonia Rizzani, Andreas Nüssler, Nobuyuki Kato, Roland M. Schmid, Asit K. Pattnaik, Oliver Eberta

School of Veterinary and Biomedical Sciences: Faculty Publications

Vesicular stomatitis virus (VSV), a negative-sense single-stranded-RNA rhabdovirus, is an extremely promising oncolytic agent for cancer treatment. Since oncolytic virotherapy is moving closer to clinical application, potentially synergistic combinations of oncolytic viruses and molecularly targeted antitumor agents are becoming a meaningful strategy for cancer treatment. Mitogenactivated protein kinase (MAPK) inhibitors have been shown to impair liver cell proliferation and tumor development, suggesting their potential use as therapeutic agents for hepatocellular carcinoma (HCC). In this work, we show that the impairment of MAPK in vitro did not interfere with the oncolytic properties of VSV in HCC cell lines. Moreover, the administration …

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Identification Of Amino Acid Residues Important For Anti-Ifn Activity Of Porcine Reproductive And Respiratory Syndrome Virus Non-Structural Protein 1, Lalit Beura, Sakthivel Subramaniam, Hiep Vu, Byungjoon Kwon, Asit K. Pattnaik, Fernando A. Osorio Jan 2012

Identification Of Amino Acid Residues Important For Anti-Ifn Activity Of Porcine Reproductive And Respiratory Syndrome Virus Non-Structural Protein 1, Lalit Beura, Sakthivel Subramaniam, Hiep Vu, Byungjoon Kwon, Asit K. Pattnaik, Fernando A. Osorio

School of Veterinary and Biomedical Sciences: Faculty Publications

The non-structural protein 1 (nsp1) of porcine reproductive and respiratory syndrome virus is partly responsible for inhibition of type I interferon (IFN) response by the infected host. By performing alanine-scanning mutagenesis, we have identified amino acid residues in nsp1α and nsp1β~ (the proteolytic products of nsp1) that when substituted with alanine(s) exhibited significant relief of IFNsuppression. A mutant virus (16-SA, in which residues 16-20 of nsp1β were substituted with alanines) encoding mutant nsp1β recovered from infectious cDNA clone was shown to be attenuated for growth in vitro and induced significantly higher amount of type I IFN transcripts in infected macrophages. …

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A Single Amino Acid Change Resulting In Loss Of Fluorescence Of Egfp In A Viral Fusion Protein Confers Fitness And Growth Advantage To The Recombinant Vesicular Stomatitis Virus, Phat X. Dinh, Debasis Panda, Phani B. Das, Subash C. Das, Anshuman Das, Asit K. Pattnaik Jan 2012

A Single Amino Acid Change Resulting In Loss Of Fluorescence Of Egfp In A Viral Fusion Protein Confers Fitness And Growth Advantage To The Recombinant Vesicular Stomatitis Virus, Phat X. Dinh, Debasis Panda, Phani B. Das, Subash C. Das, Anshuman Das, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

Using a recombinant vesicular stomatitis virus encoding eGFP fused in-frame with an essential viral replication protein, the phosphoprotein P, we show that during passage in culture, the virus mutates the nucleotide C289 within eGFP of the fusion protein PeGFP to A or T, resulting in R97S/C amino acid substitution and loss of fluorescence. The resultant non-fluorescent virus exhibits increased fitness and growth advantage over its fluorescent counterpart. The growth advantage of the non-fluorescent virus appears to be due to increased transcription and replication activities of the PeGFP protein carrying the R97S/C substitution. Further, our results show that the R97S/C mutation …

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Amino Acid Residues In The Non-Structural Protein 1 Of Porcine Reproductive And Respiratory Syndrome Virus Involved In Down-Regulation Of Tnf-Cx Expression In Vitro And Attenuation In Vivo, Sakthivel Subramaniam, Lalit Beura, Byungjoon Kwon, Asit K. Pattnaik, Fernando A. Osorio Jan 2012

Amino Acid Residues In The Non-Structural Protein 1 Of Porcine Reproductive And Respiratory Syndrome Virus Involved In Down-Regulation Of Tnf-Cx Expression In Vitro And Attenuation In Vivo, Sakthivel Subramaniam, Lalit Beura, Byungjoon Kwon, Asit K. Pattnaik, Fernando A. Osorio

School of Veterinary and Biomedical Sciences: Faculty Publications

Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses tumor necrosis factor-alpha (TNF-α) production at both transcriptional and post-transcriptional levels by its non-structural proteins 1α and 1β (Nsp1α and Nsp1β). To identifY the amino acid residues responsible for this activity, we generated several alanine substitution mutants of Nsp1α and Nsp1β. Examination of the mutant proteins revealed that Nsp1α residues Gly90, Asn91 , Arg97, Argl 00 and Arg124 were necessary for TNF-α promoter suppression, whereas several amino acids spanning the entire Nsp1β ~ were found to be required for this activity. Two mutant viruses, with mutations at Nsp1α Gly90 or Nsp1β residues …

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Staphylococcal Response To Oxidative Stress, Rosmarie Gaupp, Nagender Ledala, Greg A. Somerville Jan 2012

Staphylococcal Response To Oxidative Stress, Rosmarie Gaupp, Nagender Ledala, Greg A. Somerville

School of Veterinary and Biomedical Sciences: Faculty Publications

Staphylococci are a versatile genus of bacteria that are capable of causing acute and chronic infections in diverse host species. The success of staphylococci as pathogens is due in part to their ability to mitigate endogenous and exogenous oxidative and nitrosative stress. Endogenous oxidative stress is a consequence of life in an aerobic environment; whereas, exogenous osidative and nitrosative stress are often due to the bacteria's interaction with host immune systems. To overcome the deleterious effects of oxidative and nitrosative stress, staphylococci have evolved protection, detoxification, and repair mechanisms that are controlled by a network of regulators. In this review, …

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Serum Protein Fractions In Brazilian-Breed Donkeys Using Agarose Gel Electrophoresis, Paulo Henrique Cavalcante, Andrea Cristina Silva, Sidnei Sakamoto, Benito Soto-Blanco Jan 2012

Serum Protein Fractions In Brazilian-Breed Donkeys Using Agarose Gel Electrophoresis, Paulo Henrique Cavalcante, Andrea Cristina Silva, Sidnei Sakamoto, Benito Soto-Blanco

Turkish Journal of Veterinary & Animal Sciences

The values for the serum protein profiles of horses are available in many studies. However, only one study has characterized the electrophoretic migration profile of plasma protein in donkeys. The present study aimed to determine by agarose gel electrophoresis the normal values for serum protein fractions in erratic Brazilian-breed donkeys and to compare these values with those of the serum proteins of horses. Serum samples were collected from 26 donkeys of the Brazilian-breed and from 10 healthy horses. The serum levels of the total proteins were determined by the biuret method. The separation and determination of the values of serum …

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Caesarean Operation In Two Farmed Red Deer (Cervus Elaphus), Si̇nem Özlem Engi̇nler, Mehmet Can Gündüz, Tuğba Seval Fatma Toydemi̇r, Meli̇h Uçmak, Güven Kaşikçi Jan 2012

Caesarean Operation In Two Farmed Red Deer (Cervus Elaphus), Si̇nem Özlem Engi̇nler, Mehmet Can Gündüz, Tuğba Seval Fatma Toydemi̇r, Meli̇h Uçmak, Güven Kaşikçi

Turkish Journal of Veterinary & Animal Sciences

In this case, a caesarean section was performed on 2 red deer aged 4 and 7 who underwent dystocia. When the deer were brought to our clinic, they had been in labour for 6 h and 1 day, respectively, and the calves were dead. The foetal forefeet were found hanging from the vulva at the general examination. Deer 1's parturition was human-assisted but the outcome was not good and it was decided to operate on the animal. In deer 2, parturition was taking too long and so it was decided to operate immediately. The deer were sedated and anaesthetised by …

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Survival And Growth Of Common Carp (Cyprinus Carpio L.) Exposed To Different Water Ph Levels, Mohammad Saeed Heydarnejad Jan 2012

Survival And Growth Of Common Carp (Cyprinus Carpio L.) Exposed To Different Water Ph Levels, Mohammad Saeed Heydarnejad

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to examine the survival and growth performance of common carp (Cyprinus carpio L.) in water with different pH values. Carp (17.8 ± 1.21 cm; 52.14 ± 7.13 g) were transferred to 21 tanks previously adjusted with 7 different pH values: 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, and 9.0. Each value had 3 replicates, and all experiments lasted for 21 days. The survival and growth of carp were assessed at days 7, 14, and 21. At the end of the 21 days, greater weight, length, survival, and biomass were found for pH values of …

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Prevalence Of Escherichia Coli O157 In Red Meat And Meat Products Determined By Vidas Ecpt And Lightcycler Pcr, Seran Temelli̇, Ayşe Gül Eyi̇gör, Şahsene Anar Jan 2012

Prevalence Of Escherichia Coli O157 In Red Meat And Meat Products Determined By Vidas Ecpt And Lightcycler Pcr, Seran Temelli̇, Ayşe Gül Eyi̇gör, Şahsene Anar

Turkish Journal of Veterinary & Animal Sciences

This study aimed to determine the prevalence of Escherichia coli O157 in retail red meat and meat products with the Vitek Immunodiagnostic Assay System, including H7 Escherichia coli phage technology (VIDAS ECPT), and a real-time polymerase chain reaction system (LightCycler PCR; LCPCR). A total of 106 red meat and meat product samples were analyzed with VIDAS ECPT and LCPCR. VIDAS ECPT presumptive positive samples were subjected to VIDAS Immuno Concentration E. coli O157, followed by culture and serology. Among the 27 out of 72 (37.50%) red meat samples and 3 out of 34 (8.82%) red meat products that tested positive …

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Evaluation Of The Possibility Of Using Oligosaccharide-Free Pea In Japanese Quail Nutrition, Danuta Szczerbinska, Zofia Tarasewicz, Piotr Gulewicz, Danuta Majewska, Marek Ligocki Jan 2012

Evaluation Of The Possibility Of Using Oligosaccharide-Free Pea In Japanese Quail Nutrition, Danuta Szczerbinska, Zofia Tarasewicz, Piotr Gulewicz, Danuta Majewska, Marek Ligocki

Turkish Journal of Veterinary & Animal Sciences

In this paper, evaluation of the effect of processed pea seeds (free of raffinose family oligosaccharides) on the performance traits and the results of reproduction in Japanese quails is presented. A flock of 72 quails was divided into 3 groups consisting of equal numbers of birds (18 females and 6 males). Birds were kept in cages in an environmentally controlled room with a lighting cycle of 17 h of light and 7 h of dark, and they were fed 1 of 3 different diets. Whereas diet 1 served as the control, diet 2 was supplemented with 5% and diet 3 …

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Molecular And Kinetic Characterization Of Lactate Dehydrogenase Enzyme In The Heart And Breast Muscle Of Japanese Quail (Coturnix Japonica), Ram Pratap Singh, Kochiganti Venkata Hanumat Sastry, Radha Agarwal, Nitin Kumar Pandey, Vishesh Kumar Saxena, Jag Mohan Jan 2012

Molecular And Kinetic Characterization Of Lactate Dehydrogenase Enzyme In The Heart And Breast Muscle Of Japanese Quail (Coturnix Japonica), Ram Pratap Singh, Kochiganti Venkata Hanumat Sastry, Radha Agarwal, Nitin Kumar Pandey, Vishesh Kumar Saxena, Jag Mohan

Turkish Journal of Veterinary & Animal Sciences

In the present investigation, attempts were made to characterize lactate dehydrogenase (LDH) isozyme in quail heart and breast muscle by using electrophoresis, kinetic, and molecular techniques. Polyacrylamide gel electrophoresis of the heart and breast muscle revealed a single isozyme. Kinetic studies were carried out for further functional characterization of LDH isozyme in the heart and breast muscle. Substrate inhibition, enzyme kinetics, and inhibitor (urea) studies indicated that the isozymes present in these tissues differed in their functional properties. LDH isozyme present in the heart and breast muscle exhibited properties of LDH-A and LDH-B isozyme, respectively. Furthermore, RT-PCR amplification of Ldh-a …

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