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2011

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Articles 31 - 60 of 222

Full-Text Articles in Veterinary Medicine

Infectious Cdna Clone Of The Modified Live Virus Vaccine Strain Of Equine Arteritis Virus, Udeni B. R. Balasuriya, Peter J. Timoney, Jianquan Wang Sep 2011

Infectious Cdna Clone Of The Modified Live Virus Vaccine Strain Of Equine Arteritis Virus, Udeni B. R. Balasuriya, Peter J. Timoney, Jianquan Wang

Veterinary Science Faculty Patents

An isolated polynucleotide molecule includes a DNA sequence encoding an infectious RNA molecule encoding a modified live viral strain of an Equine arteritis virus, wherein the DNA sequence is SEQ ID NO:1 or a degenerate variant thereof. Also provided are transformed or transfected host cells including that sequence, vectors including the sequence, and isolated infectious RNA molecules encoded by the sequence. Further, a modified DNA sequence encoding an infectious RNA molecule encoding a modified live viral strain of an Equine arteritis virus is provided wherein the DNA sequence is SEQ ID NO:2 or a degenerate variant thereof, including a silent …

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The Efficacy Of Three Types Of Analgesic Drugs In Reducing Pain In The Rainbow Trout, Oncorhynchus Mykiss, Jessica J. Mettam, Lois J. Oulton, Catherine R. Mccrohan, Lynne U. Sneddon Sep 2011

The Efficacy Of Three Types Of Analgesic Drugs In Reducing Pain In The Rainbow Trout, Oncorhynchus Mykiss, Jessica J. Mettam, Lois J. Oulton, Catherine R. Mccrohan, Lynne U. Sneddon

Veterinary Science and Medicine Collection

Recent research has shown the possibility of pain perception in fish; therefore, the use of analgesia or “painkillers” should be considered for invasive procedures. However, there is relatively little information on the effectiveness of analgesic drugs nor on the appropriate dose for fish. This study assessed the efficacy of three types of drug: an opioid, buprenorphine, a non-steroidal anti-inflammatory drug (NSAID), carprofen and a local anaesthetic, lidocaine. Each drug was tested at three doses on rainbow trout that were noxiously stimulated and the most effective dose was also given to fish experiencing no pain to investigate side-effects. Ventilation rate and …

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Evaluation Of Microsatellite Polymorphism And Genetic Variability In Thai Ridgeback And Bangkaew Dogs, Janjira Phavaphutanon, Sudtisa Laopiem Sep 2011

Evaluation Of Microsatellite Polymorphism And Genetic Variability In Thai Ridgeback And Bangkaew Dogs, Janjira Phavaphutanon, Sudtisa Laopiem

The Thai Journal of Veterinary Medicine

The origin of dogs came from the limited number of founders leading to inbreeding or genetic diseasewithin or among breeds. Thai Ridgeback (TR) and Bangkaew (BK) dogs were the only native dog breeds that webelieved to originate in Thailand. The study of genetic variability in Thai dogs (39 Thai Ridgeback dogs, 82 Bangkaewdogs) was defined by using 12 microsatellite markers. These DNA markers were highly informative and distributedthrough the canine genome. The result from these markers revealed the higher number of alleles, observedheterozygosity (Ho), expected heterozygosity (He) and polymorphic information content (PIC) in TR and BK dogs.The average Ho, He …

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Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct, Paisan Tienthai Sep 2011

Hyaluronan And Syndecan-1 Localization In The Swamp Buffalo Oviduct, Paisan Tienthai

The Thai Journal of Veterinary Medicine

The glycosaminoglycans (GAGs) found in the mammalian oviductal tissue and luminal secretory fluidinfluence the fertilization processes in various patterns such as sperm storage, viability and capacitation. The aim ofthis study was to confirm the appearance of hyaluronan and syndecan-1 in the swamp buffalo oviduct consisting ofuterotubal junction (UTJ), isthmus, ampulla and infundibulum during oestrous cycle using histochemical andimmunohistochemical techniques. The non-sulphated GAGs, i.e. hyaluronan (HA), were noticeably localized in thesubepithelial connective tissue layer or lamina propria of all segments while the epithelial HA-positive staining wasdemonstrated only in the UTJ and isthmus (sperm reservoir) at the follicular phase. In contrast, the …

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Ecg Quiz, Chollada Buranakarl Sep 2011

Ecg Quiz, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

No abstract provided.

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Ophthalmology Snapshot, Nalinee Tuntivanich Sep 2011

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

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What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep Sep 2011

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

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Antagonistic Effects Of Cellular Poly(C) Binding Proteins On Vesicular Stomatitis Virus Gene Expression, Phat X. Dinh, Lalit Beura, Debasis Panda, Anshuman Das, Asit K. Pattnaik Sep 2011

Antagonistic Effects Of Cellular Poly(C) Binding Proteins On Vesicular Stomatitis Virus Gene Expression, Phat X. Dinh, Lalit Beura, Debasis Panda, Anshuman Das, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

Immunoprecipitation and subsequent mass spectrometry analysis of the cellular proteins from cells expressing the vesicular stomatitis virus (VSV) P protein identified the poly(C) binding protein 2 (PCBP2) as one of the P protein-interacting proteins. To investigate the role of PCBP2 in the viral life cycle, we examined the effects of depletion or overexpression of this protein on VSV growth. Small interfering RNA-mediated silencing of PCBP2 promoted VSV replication. Conversely, overexpression of PCBP2 in transfected cells suppressed VSV growth. Further studies revealed that PCBP2 negatively regulates overall viral mRNA accumulation and subsequent genome replication. Coimmunoprecipitation and immunofluorescence microscopic studies showed that …

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Acute Fatty Liver Of Pregnancy: An Update On Mechanisms, Sathish Kumar Natarajan, Kavitha R. Thangaraj, Ashish Goel, C. E. Eapen, K. A. Balasubramanian, Anup Ramachandran Sep 2011

Acute Fatty Liver Of Pregnancy: An Update On Mechanisms, Sathish Kumar Natarajan, Kavitha R. Thangaraj, Ashish Goel, C. E. Eapen, K. A. Balasubramanian, Anup Ramachandran

School of Veterinary and Biomedical Sciences: Faculty Publications

Acute fatty liver of pregnancy (AFLP), characterized by hepatic microvesicular steatosis, is a sudden catastrophic illness occurring almost exclusively in the third trimester of pregnancy. Defective fatty acid oxidation in the fetus has been shown to be associated with this disease. Since the placenta has the same genetic makeup as the fetus and as AFLP patients generally recover following delivery, we hypothesized that the placenta might be involved in pathogenesis of this disease. In an animal model of hepatic microvesicular steatosis (using sodium valproate), we found that microvesicular steatosis results in mitochondrial structural alterations and oxidative stress in subcellular organelles …

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World Veterinary Year 2011 250 Years Veterinary Education In The World, Achariya Sailasuta Sep 2011

World Veterinary Year 2011 250 Years Veterinary Education In The World, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

No abstract provided.

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Investigation Of The Relationship Between Endometrial Mmp Activities And Sex Hormones In Mature Bitches, Wei-Yau Shia, Po-Chen Chu, Chih-Chung Chou, Wei-Ming Lee Sep 2011

Investigation Of The Relationship Between Endometrial Mmp Activities And Sex Hormones In Mature Bitches, Wei-Yau Shia, Po-Chen Chu, Chih-Chung Chou, Wei-Ming Lee

The Thai Journal of Veterinary Medicine

Endometrial matrix metalloproteinases (MMPs) are extensively studied in humans. However, owing to thedistinct differences of the estrous cycle of bitches, the expression and activities of endometrial MMPs regulated by sexhormones should be significantly different. To understand the effect of sex hormones on the activity of MMPs, thisstudy investigates the activities of endometrial MMP-2 and MMP-9 during different phases of the estrous cycle inbitches through the use of gelatine zymography. Estradiol and progesterone concentrations were also determinedusing enzyme-linked fluorescent assay. The activities of the latent forms of both MMP-2 and -9 were significantlyhigher in metestrus (MP) and late diestrus phases (L-DP) …

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Analysis On Differentially Expressed Genes Of Muscle Tissues In Rugao Chicken At The Ages Of 2 And 12 Weeks By Microarray, Qin De Luan, Guo Bin Chang, Ting Zhen, Zheng Yang Huang, Wei Zhou, Yan Liu, Guo Hong Chen Sep 2011

Analysis On Differentially Expressed Genes Of Muscle Tissues In Rugao Chicken At The Ages Of 2 And 12 Weeks By Microarray, Qin De Luan, Guo Bin Chang, Ting Zhen, Zheng Yang Huang, Wei Zhou, Yan Liu, Guo Hong Chen

The Thai Journal of Veterinary Medicine

Microarray containing 13319 probes was used to construct gene expression profiles in order to screendifferentially expressed genes of muscle tissues in Rugao chicken and investigate the molecular mechanism relatedwith muscle tissue traits at 2 and 12 weeks old of chicken. Two hundred and eight differentially expressed genes, ofwhich 108 were known genes, 94 up-regulated, 114 down-regulated were involved in growth, molecular mechanism,fat metabolism, cell proliferation, transcription and splicing factor, protein synthesis and degradation. Meanwhile,some genes of these differentially expressed genes that had no annotation in GenBank were screened out, they werepresumed to be unknown new genes. The differential expressions of …

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Seroprevalence And Risk Factors Associated With Caprine Arthritis-Encephalitis Virus Infection In Goats In The Western Part Of Thailand, Thant Nyi Lin, Saroch Ngarmkum, Kanisak Oraveerakul, Prachin Virakul, Mongkol Techakumphu Sep 2011

Seroprevalence And Risk Factors Associated With Caprine Arthritis-Encephalitis Virus Infection In Goats In The Western Part Of Thailand, Thant Nyi Lin, Saroch Ngarmkum, Kanisak Oraveerakul, Prachin Virakul, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

To determine the seroprevalence of caprine arthritis-encephalitis virus infection (CAEV) in goats in theWestern part of Thailand, a cross-sectional serological survey was conducted in three provinces, Ratchaburi,Petchaburi and Kanchanaburi, situated in the western part of the country along the border area. A total of 1,129serum samples from 74 randomly selected goat farms containing different breeds of goats were collected during theperiod from November 2009 to January 2011. Seroprevalence of CAEV antibodies was determined using competitiveenzyme-linked immunosorbent assay (cELISA) test. To investigate the risk factors associated with the seroprevalenceof CAEV antibodies, semi-structural questionnaires were developed and presented to farm owners to …

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Effects Of Dietary Protein Deficiency On Plasma Amino Acids And Physical – Histological Conformation Of The Mammary Glands In Lactating Sows, Patrapan Rungcharoen, Sarinee Kalandakanond-Thongsong, Boonrit Thongsong Sep 2011

Effects Of Dietary Protein Deficiency On Plasma Amino Acids And Physical – Histological Conformation Of The Mammary Glands In Lactating Sows, Patrapan Rungcharoen, Sarinee Kalandakanond-Thongsong, Boonrit Thongsong

The Thai Journal of Veterinary Medicine

Eleven multiparous crossbred lactating sows were used to determine the influence of deficient protein dietcontaining 8.2% crude protein and normal protein diet containing 18.2% crude protein on plasma amino acidconcentrations of sows and their piglets, physical mammary gland and histological changes of mammary cells. Theywere provided sufficient water and feed access to one of two isocaloric diets, either deficient (6 sows) or normalprotein diets (5 sows). The experimental period started from farrowing (day 0) until weaning day (day 28). In thisstudy, sows fed deficient protein diet had significant increase in body weight losses and their piglets had significantdecrease in body …

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Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo, Aunyarattana Thontiravong, Tilladit Rung-Ruangkijkrai, Pravina Kitikoon, Kanisak Oraveerakul, Yong Poovorawan Sep 2011

Influenza A Virus Receptor Identification In The Respiratory Tract Of Quail, Pig, Cow And Swamp Buffalo, Aunyarattana Thontiravong, Tilladit Rung-Ruangkijkrai, Pravina Kitikoon, Kanisak Oraveerakul, Yong Poovorawan

The Thai Journal of Veterinary Medicine

Virus infection requires the interaction of a virus protein with the host-cell receptor. The Influenza A virusreceptor consists of sialic acid linked to the galactose unit in a α 2,3 or α 2,6 conformation. Various types of thesereceptors are expressed differently on the epithelial lining of various animal species. Here we characterized the typesof receptors that are expressed in the upper and lower respiratory tracts of cow, buffalo, pig and quail. Our findingsdemonstrate that SA α 2,6-gal linked receptors for human influenza viruses are present in the lower respiratory tractof cow and buffalo, while the SA α 2,3-gal linked receptors …

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Survey Of Enterococcus Faecalis In Diseased Swine In Hunan Province, China, Wei Jiang, Shi-Cheng Tian, Run-Cheng Li, Yun-Qiu Yan, Ying-Zi Wang, Man-Xiang Li Sep 2011

Survey Of Enterococcus Faecalis In Diseased Swine In Hunan Province, China, Wei Jiang, Shi-Cheng Tian, Run-Cheng Li, Yun-Qiu Yan, Ying-Zi Wang, Man-Xiang Li

The Thai Journal of Veterinary Medicine

The prevalence of Enterococcus faecalis was investigated in diseased swine in Hunan Province, China betweenFebruary 2007 and February 2011. A total of 385 diseased swine dissected in prosectorium from 10 representativeadministrative regions in Hunan Province were examined for the presence of E. faecalis. Forty-two out of 385 (10.91%)diseased swine were found to be infected with E. faecalis by bacteria isolations. E. faecalis was mainly recovered fromthe lung and joint cavity. Identification of the organisms was based on biochemical assays and sequencing of thepartial 16S rRNA. The results of the present investigation have implications for the ongoing control of E. faecalisinfections …

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Ultrasound Diagnosis, Phiwipha Kamonrat Sep 2011

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

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Observation Of Ruminococcus Strains In Captive Asian Elephant (Elephas Maximus), Nattawooti Sthitmatee, Pranisa Mahatnirunkul, Patcharaporn Keawmong, Anucha Sirimalaisuwan, Pinich Boontong, Suvichai Rojanasthien, Sumalee Boonmar Sep 2011

Observation Of Ruminococcus Strains In Captive Asian Elephant (Elephas Maximus), Nattawooti Sthitmatee, Pranisa Mahatnirunkul, Patcharaporn Keawmong, Anucha Sirimalaisuwan, Pinich Boontong, Suvichai Rojanasthien, Sumalee Boonmar

The Thai Journal of Veterinary Medicine

Asian elephant is indigenous to many countries including Thailand, but fermenter microorganisms ingastrointestinal tract of the elephant have not fully been investigated. Therefore, this study aimed to observe thecellulolytic bacteria in Genus Ruminococcus in large intestines of captive Asian elephants (Elephas maximus). Fecalsamples were collected from male and female sucklings, young and adult captive Asian elephants. Forty-fourelephants were divided into 3 groups as followed: A) > 18 years old (n=24); B) 2-18 years old (n=17) and C) < 2 yearsold (n=3). The results revealed that there were 214 (42.8%) isolates of R. flavefaciens, 105 (21.0%) isolates of R. bromii, 90(18.0%) isolates of R. obeum, 54 (10.8%) isolates of R. callidus and 37 (7.4%) isolates of R. albus from all fecal samplesexamined. Interestingly, Ruminococcus strains could be isolated from the weaned elephants, but were not found in thesucklings (p<0.05). In conclusion, cellulolytic bacteria in Genus Ruminococcus were isolated from the large intestines ofcaptive Asian elephants. Moreover, the highest prevalence of the bacteria was found in the elephants aged more than18 years old.

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The Effect Of Using Long Term And Short Term Extenders During Cooling Process On The Quality Of Frozen Boar Semen, Kampon Kaeoket, Primsiri Chanapai, Puriwat Junchiyaphoom, Panida Chanapiwat Sep 2011

The Effect Of Using Long Term And Short Term Extenders During Cooling Process On The Quality Of Frozen Boar Semen, Kampon Kaeoket, Primsiri Chanapai, Puriwat Junchiyaphoom, Panida Chanapiwat

The Thai Journal of Veterinary Medicine

Cooling process of cryopreservation is one of the important factors that affect the qualities of frozen-thawedboar semen. The objective of this study was to compare the qualities of frozen-thawed boar semen after usingdifferent extenders (i.e. BTS, Vitasem LD, ModenaTM and Androstar® plus) during cooling process. Eight sperm-richfractions from 8 boars were employed. The ejaculated semen sample from each boar was divided into four groupsand extended in different freezing extender I as follows: group I (control, short term, BTS), group II (long term,Vitasem LD), group III (long term, ModenaTM) and group IV (long term, Androstar® plus) and kept at 15oC for …

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Effect Of Water Temperature On Susceptibility To Streptococcus Agalactiae Serotype Ia Infection In Nile Tilapia (Oreochromis Niloticus), Channarong Rodkhum, Pattanapon Kayansamruaj, Nopadon Pirarat Sep 2011

Effect Of Water Temperature On Susceptibility To Streptococcus Agalactiae Serotype Ia Infection In Nile Tilapia (Oreochromis Niloticus), Channarong Rodkhum, Pattanapon Kayansamruaj, Nopadon Pirarat

The Thai Journal of Veterinary Medicine

Streptococcus agalactiae is one of the causative agents associated with warm-water streptococcosis, in whichproduce massive mortality in aquaculture. Emergence of disease in tilapia farm usually occurs in high temperatureseason, which suggested for higher susceptibility of tilapia in this particular condition. Thus, the objective of thisstudy is to investigate the association between water temperature and susceptibility of Nile tilapia (Oreochromisniloticus) to S. agalactiae serotype Ia infection. Nile tilapia were inoculated with 108, 107 or 106 CFU/ml of S. agalactiaeserotype Ia field strain via water immersion route and maintained in different water temperature at 25, 30 or 33oC for1 week. Diseased fish …

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Differences Of Periods Of Calving On Days Open Of Dairy Cows In Different Regions And Months Of Thailand, Winai Kaewlamun, Rut Chayaratanasin, Prachin Virakul, Andrew A. Ponter, Patrice Humblot, Siriwat Suadsong, Padet Tummaruk, Mongkol Techakumphu Sep 2011

Differences Of Periods Of Calving On Days Open Of Dairy Cows In Different Regions And Months Of Thailand, Winai Kaewlamun, Rut Chayaratanasin, Prachin Virakul, Andrew A. Ponter, Patrice Humblot, Siriwat Suadsong, Padet Tummaruk, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The objectives of this study were to analyze the potential impact of heat stress on reproduction in differentregions of Thailand, to determine the monthly distribution of calving throughout the year and to investigateenvironmental sources of variation of days-open (DO) in first lactation dairy cows. Data including 13,548 lactationrecords were collected during 2004 to 2006. Climate data were obtained from the provincial meteorological stationsand the corresponding temperature-humidity indexes were calculated. The difference between regions in THI wasdetermined. The geographical regions studied were: Central, Eastern, Northeastern, and Northern. The distributionof calving by month was determined in the 1st to 5th lactation. The …

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Prevalence And Genotype Of Salmonella Choleraesuis In Gunma Prefecture, Japan, Chieko Sakano, Yukio Morita, Koichi Goto, Yoko Yokota, Hideyuki Annaka, Masahiro Fujita, Satoshi Kobatake, Taisei Ishioka, Toshie Hoshino, Sumalee Boonmar, Chaiwat Pulsrikarn, Atsuyoshi Nishina, Kunihisa Kozawa, Shigeki Yamamoto, Hirokazu Kimura Sep 2011

Prevalence And Genotype Of Salmonella Choleraesuis In Gunma Prefecture, Japan, Chieko Sakano, Yukio Morita, Koichi Goto, Yoko Yokota, Hideyuki Annaka, Masahiro Fujita, Satoshi Kobatake, Taisei Ishioka, Toshie Hoshino, Sumalee Boonmar, Chaiwat Pulsrikarn, Atsuyoshi Nishina, Kunihisa Kozawa, Shigeki Yamamoto, Hirokazu Kimura

The Thai Journal of Veterinary Medicine

We studied the prevalence of swine salmonellosis and PFGE genotype of isolates in Gunma Prefecture,Japan. Between 2005 and 2008, swine salmonellosis was confirmed in 430 of 2,707,402 (0.02%) swine atslaughterhouses. All isolates were identified as deriving from Salmonella Choleraesuis, biotype Choleraesuis (negativefor H2S production). We used 30 bacterial strains from 15 farms that had experienced outbreaks in 2006 and 2007. Allstrains were susceptible to various antibiotics such as cephems (cefotaxime), fluoroquinolones (norfloxacin andciprofloxacin), and fosfomycin. On the other hand, all strains were resistant to tetracycline (TC), and 29 of 30 (97%)strains were resistant to streptomycin (SM). The most predominant profiles …

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Effect Of Roselle (Hibiscus Sabdariffa Linn.) Calyx In Laying Hen Diet On Egg Production Performance, Egg Quality And Tbars Value In Plasma And Yolk, Piyaphon Sukkhavanit, Kris Angkanaporn, Suwanna Kijparkorn Sep 2011

Effect Of Roselle (Hibiscus Sabdariffa Linn.) Calyx In Laying Hen Diet On Egg Production Performance, Egg Quality And Tbars Value In Plasma And Yolk, Piyaphon Sukkhavanit, Kris Angkanaporn, Suwanna Kijparkorn

The Thai Journal of Veterinary Medicine

An experiment was conducted to investigate the effect of Roselle calyx in two preparation forms in layerdiets on egg production performance, egg quality and TBARS value in plasma and yolk. Two hundred and seventy,33-week-old, CP Brown laying hens were randomly allocated into 6 treatments with 5 replications of 9 hens each.The dietary treatments were control diet, control diet supplemented with 250 mg/kg α-tocopheryl acetate, dietcontaining 1 and 2% of Roselle calyx crude extracts, and diet containing 2% and 4% Roselle calyx powder. Eggproduction performance was recorded, egg quality was measured and TBARS value in plasma and yolk wereanalyzed after fed …

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Effect Of Equex Stm Paste On The Quality And Motility Characteristics Of Post Thawed Cryopreserved Goat Semen, Nitira Anakkul, Junpen Suwimonteerabutr, Jinda Singlor, Nawapen Phutikanit, Theerawat Tharasanit, Mongkol Techakumphu Sep 2011

Effect Of Equex Stm Paste On The Quality And Motility Characteristics Of Post Thawed Cryopreserved Goat Semen, Nitira Anakkul, Junpen Suwimonteerabutr, Jinda Singlor, Nawapen Phutikanit, Theerawat Tharasanit, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The aim of this study was to evaluate the effect of Equex STM Paste on goat semen quality aftercryopreservation. Semen samples were consecutively collected once a week, from six mature bucks. The semensamples with adequate motility (>60%) and sperm concentration (>1,500x106 sperm/ml) were frozen in an egg yolk-Tris semen extender supplemented either with (E+) or without (E-) Equex STM Paste. The semen quality includingmotility, viability, morphology, acrosome integrity and membrane functional integrity were evaluated beforeprocessing and after cryopreservation. Computer-assisted-semen analysis (CASA) was used to characterize the spermmotion patterns during the thermal resistance test at 0, 1, 2 and …

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Detection Of West Nile Virus In Mosquitoes In Nakhon-Pathom And Phetchaburi Province, Thailand, Kriangkrai Witoonsatian, Nuananong Sinwat, Rungroj Jam-On, Khongsak Thiangtum, Thaweesak Songserm Sep 2011

Detection Of West Nile Virus In Mosquitoes In Nakhon-Pathom And Phetchaburi Province, Thailand, Kriangkrai Witoonsatian, Nuananong Sinwat, Rungroj Jam-On, Khongsak Thiangtum, Thaweesak Songserm

The Thai Journal of Veterinary Medicine

West Nile (WN) disease is one of the encephalitic diseases in humans and mammals. This disease has beenreported in several areas of the world. Thailand is located in the area of high risk of WN virus (WNv) infectionconcerning a natural transmission cycle. However, the WNv infection has not been previously reported. Therefore,the aim of this study was to detect WNv in mosquitoes in some areas in Nakhon-pathom and Phetchaburi provinces,central Thailand. The period of this study was done during November 2008 to November 2010. We found that therewere four genera collected including Aedes, Anopheles, Culex and Mansonia. Culex spp such …

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Comparison Of Genomic And Proteomic Data In Recurrent Airway Obstruction Affected Horses Using Ingenuity Pathway Analysis, Julien Racine, Vinzenz Gerber, Marybeth Miskovic Feutz, C Paige Riley, Jiri Adamec, June E. Swinburne, Laurent Couetil Aug 2011

Comparison Of Genomic And Proteomic Data In Recurrent Airway Obstruction Affected Horses Using Ingenuity Pathway Analysis, Julien Racine, Vinzenz Gerber, Marybeth Miskovic Feutz, C Paige Riley, Jiri Adamec, June E. Swinburne, Laurent Couetil

Department of Comparative Pathobiology Faculty Publications

Background: Recurrent airway obstruction (RAO) is a severe chronic respiratory disease affecting horses worldwide, though mostly in the Northern hemisphere. Environmental as well as genetic factors strongly influence the course and prognosis of the disease. Research has been focused on characterization of immunologic factors contributing to inflammatory responses, on genetic linkage analysis, and, more recently, on proteomic analysis of airway secretions from affected horses. The goal of this study was to investigate the interactions between eight candidate genes previously identified in a genetic linkage study and proteins expressed in bronchoalveolar lavage fluid (BALF) collected from healthy and RAO-affected horses. The …

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Effect Of The Infection With Porcine Reproductive And Respiratory Syndrome Virus On The Regulation Of Cytokines: Tumor Necrosis Factor-Alpha And Interleukin-10, Sakthivel Subramaniam Aug 2011

Effect Of The Infection With Porcine Reproductive And Respiratory Syndrome Virus On The Regulation Of Cytokines: Tumor Necrosis Factor-Alpha And Interleukin-10, Sakthivel Subramaniam

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Porcine reproductive and respiratory syndrome virus (PRRSV) causes late-term abortion in sows and pneumonia in growing piglets. PRRSV evades the host immune response by several mechanisms, including the modulation of cytokine secretions in infected pigs, which is the subject of this dissertation. Particularly, PRRSV reduces the secretion of the pro-inflammatory cytokine, tumor necrosis factor-α (TNF-α) but increases the secretion of the anti-inflammatory cytokine interleukin-10 (IL-10). The latter effect, however, is PRRSV strain-specific. In this dissertation, we have examined mechanisms by which PRRSV regulates TNF-α and IL-10 expressions. The pathogenic strain FL12, derived from a PRRSV infectious clone, consistently suppressed TNF-α …

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Role Of Porcine Reproductive And Respiratory Syndrome Virus Non-Structural Protein 1 In Viral Replications And Pathogenesis, Lalit Kumar Beura Aug 2011

Role Of Porcine Reproductive And Respiratory Syndrome Virus Non-Structural Protein 1 In Viral Replications And Pathogenesis, Lalit Kumar Beura

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Porcine reproductive and respiratory syndrome virus (PRRSV) is an infectious agent of significant concern to the global swine industry. PRRSV infection of pigs is initiated by a long viremia after which the virus enters in an extended persistent phase of 3-4 months that ultimately is resolved by the immune system. The delayed and weak host adaptive response is responsible for the protracted convalescence period. An initial sub-optimal innate immune response is postulated to be the reason behind such meager adaptive immune response. A major focus of the studies undertaken in this dissertation was to identify the viral non-structural proteins (nsps) …

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Identification Of Host Proteins Required For Vesicular Stomatitis Virus Infection, Debasis Panda Aug 2011

Identification Of Host Proteins Required For Vesicular Stomatitis Virus Infection, Debasis Panda

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Viruses usurp host cell pathways for different stages of their infection. Understanding virus-host interaction will be invaluable to elucidate molecular mechanisms of virus infection and to identify drug targets. In order to identify such critical cellular genes in vesicular stomatitis virus (VSV, a model non-segmented negative strand RNA virus) infection, we developed a stable cell line constitutively expressing replication proteins of VSV. Attempts to establish a cell line replicating a sub-genomic replicon was not successful because of induction of interferon response by replication of viral genomic analog. Subsequently, we used siRNA technology and conducted a genome-wide siRNA screen in HeLa …

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Glutaredoxin 2 Prevents H2o2-Induced Cell Apoptosis By Protecting Complex I Activity In The Mitochondria, Hongli Wu, Kuiyi Xing, Marjorie F. Lou Aug 2011

Glutaredoxin 2 Prevents H2o2-Induced Cell Apoptosis By Protecting Complex I Activity In The Mitochondria, Hongli Wu, Kuiyi Xing, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Glutaredoxin 2 (Grx2) belongs to the oxidoreductase family and is an isozyme of glutaredoxin 1 (Grx1) present in the mitochondria, however its function is not well understood. The purpose of this study is to evaluate the potential anti-apoptotic function of Grx2 by examining its ability to protect complex I in the mitochondrial electron transport system using human lens epithelial cells as a model. We found that cells treated with 200 μM hydrogen peroxide (H2O2) for 24 h exhibited decreased viability and became apoptotic with corresponding Bax up-regulation, Bcl-2 down-regulation, caspase 3 activation and mitochondrial cytochrome c leakage. Grx2 over-expression (OE) …

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