Veterinary Medicine Commons^™

Association Analysis Of Aquaporin 7 (Aqp7) Gene Variants With Semen Quality And Fertility In Bulls, Tenghe Ma, Jifeng Liu, Ruifeng Zhao, Hao Jiang, Lisheng Dai, Yumin Zhao, Zhihui Zhao, Jiabao Zhang

Turkish Journal of Veterinary & Animal Sciences

Aquaporin 7 (AQP7) gene as a candidate Antifreeze gene was investigated and associated with fresh and frozen semen quality traits in 45 Simmental and Charolais bulls. PCR-SSCP, PCR-RFLP, and sequencing method were employed to detect the single nucleotide polymorphisms (SNPs) of the AQP7 gene. Two mutations, A264G and G371C, were identified. The allele frequencies were A (0.97, 0.96) to B (0.03, 0.04) and C (0.97, 0.91) to D (0.03, 0.09) in Simmental and Charolais bulls, respectively. A264G substitution was significantly associated with acrosome integrity ratio (P < 0.01) and motility (P < 0.05) in frozen semen. Meanwhile, G371C substitution was significantly associated with acrosome integrity ratio (P < 0.05), percentages of viable sperm (P < 0.05), and motility (P < 0.01) in frozen semen. The results prove that AQP7 gene SNPs are useful for marker-assisted selection in early seed selection in the bull industry.

Alteration Of Gene Expression In Microvascular Endothelial Cells After Exposure To Foot-And-Mouth Disease Virus 146s Antigen, Tao Zhang, Shuang Zhang, Li-Fang Tian, Ge Hu, Zhan-Wei Suo, Wu Chen, Hui-Qin Duan, Xiang Mu, Ke-Dao Teng

Turkish Journal of Veterinary & Animal Sciences

To investigate the global response of microvascular endothelial cells (MVECs) to foot-and-mouth (FMDV)disease vaccine (FMDV) antigen and to explore the role of MVECs in FMD vaccine immunity, the gene expression alterations of rat myocardium microvascular endothelial cells (RMMVECs) after stimulation with FMD virus (FMDV) 146S antigen were assayed using cDNA microarrays. We observed that 18 genes and 67 genes in RMMVECs were respectively altered by FMDV 146S antigen at 6 h and 24 h, respectively. The former were all up-regulated genes, and the latter included 60 up-regulated genes and 7 down-regulated genes. Among the differentially expressed genes, many were associated …

Duration Of Immunity Following The Administration Of Oil-Based Avian Influenza H_5n_1 Vaccine In Layers, Maryam Nisar, Anwar-Ul Haq, Asif Rashid, Muhammad Iqbal

Turkish Journal of Veterinary & Animal Sciences

Avian influenza (AI) occurs worldwide and causes tremendous economic losses. The disease is characterised by respiratory signs, depression, and reduced food and water intake. In the present study, an oil-based vaccine created by using Montanide ISA 70 MVG, was prepared and the duration of immunity checked at different time intervals. For this purpose, the cumulative mean titre (CMT) was calculated after employing haemagglutination inhibition test in 50 pullets at day zero before vaccination and then inoculated 0.3 mL of emulsified vaccine subcutaneously in 40 birds while 10 birds were kept non-vaccinated as a control group throughout the experiment. On day …

Effects Of Dietary Fish Oil Replacement By Cottonseed Oil On Growth Performance And Fatty Acid Composition Of Rainbow Trout (Oncorhynchus Mykiss), Müjde Güler, Mustafa Yildiz

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to determine the effects of replacing fish oil (FO) with cottonseed oil (CSO), as an alternative plant lipid source in diets for rainbow trout (89.3 ± 1.1 g mean individual weight), on the growth and fatty acid composition of fish. As a control diet, 5 experimental diets were formulated with pure FO and with partial or complete (25%, 50%, 75%, or 100% CSO, respectively) replacement of FO with CSO. The fish were fed 2% of their body weight per day for 60 days at an average water temperature of 10.2 °C. At the …

Monitoring Of Somatic Cell Count Variations During Lactation In Primiparous And Multiparous Turkish Saanen Goats (Capra Hircus), Abdülkadi̇r Orman, Ayteki̇n Günay, Faruk Balci, Mehmet Koyuncu

Turkish Journal of Veterinary & Animal Sciences

In this study, a total of 28 Saanen does were used to evaluate the relationship between somatic cell count (SCC) and milk yield. The study included 14 first-lactation (primiparous) does and 14 does in second lactation or greater (multiparous). Individual milk samples from both morning and evening milkings were taken every 15 days throughout the lactation period of 7 months. While somatic cell counts were higher during the evening milking (840.40 versus 532.30 cells mL-1; 1050.40 versus 759.70 cells mL-1), milk yields were lower during the evening milking (115.40 versus 193.80 kg; 177.40 versus 280.00 kg) for both primiparous and …

Population Ecology Of A Phthirapteran Occurring On The Common Baya (Ploceus Philippinus) (Ploceidae: Passeriformes: Aves), Gaurav Arya, Nayanci Bansal, Aftab Ahmad, Nidhi Gupta, Arun Kumar Saxena

Turkish Journal of Veterinary & Animal Sciences

One hundred common Bayas (Ploceus philippinus) were examined for their ectoparasites, during April 2006 to December 2008 in the Rampur district of India (28°49'12"N 79°1'11"E). Seventy-four animals were found infested (mean intensity 13.97, sample mean abundance 10.3, range of infestation 1-45) with an ischnoceran louse, Brueelia plocea. The frequency distribution pattern of the louse on the aforesaid bird was aggregated but did not conform to the negative binomial model. The sex ratio was female biased while the nymphal population was dominant over adults.

Comparison Of Placentome Diameters In Single And Twin-Pregnant Sheep By Ultrasonographic Method, Güven Kaşikçi, Özge Yilmaz, Mehmet Can Gündüz, İsmai̇l Kirşan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the correlation between fetal number and placentome diameters in pregnant sheep. In the present study 25 Kıvırcık mixed breed pregnant ewes were used. Pregnancy of the ewes was diagnosed by transrectal ultrasonography by the 25th day after insemination. A transabdominal probe (6.5 MHz) was used on the pregnant ewes at the 30th day. At this time the placentomes appeared as hypoechogenic areas with a "C"-like shape. Throughout the pregnancies 5 placentomes were measured from each ewe every week. The results showed no significant difference between placentome diameter size in single and twin-pregnant …

Effects Of Halving Intravaginal Sponges And Ecg Dose On Estrus Response And Fertility In Tahirova Ewes During The Breeding Season, Güven Kaşikçi, Ümüt Ci̇ri̇t, Mehmet Can Gündüz, Süleyman Bacinoğlu, Ahmet Sabuncu

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare the effect of halving the intravaginal sponges impregnated with 20 mg of flugestone acetate (FGA; 20 or 10 mg) and a dose of equine chorionic gonadotropin (eCG; 600 or 300 IU) on fertility in Tahirova ewes. A total of 200 Tahirova ewes were randomly assigned to 1 of 2 groups during the breeding season. Half of the ewes (n = 100) were treated with whole intravaginal sponges (WS, 20 mg of FGA) and the other half (n = 100) with halved sponges (HS, 20 mg of FGA) for 14 days. The ewes …

Comparative Ultrasonographic Study Of The Prostate Complex And Bulbourethral Glands Of The Domestic Rabbit (Oryctolagus Cuniculus), Rosen Dimitrov, Kamelia Stamatova

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to provide a comparative description of the rabbit prostate complex and bulbourethral glands by means of ultrasonography, in order to obtain results that may be useful for the diagnosis of reproductive pathology in this species. The prostate and bulbourethral glands were studied sonographically in 12 sexually mature, clinically healthy male New Zealand rabbits that were 12 months old and weighed 2.8 to 3.2 kg. The prostate complex, composed of proprostate, prostate, and paraprostate parts, was observed using prepubic ultrasonography in sagittal and transversal planes and presented a hyperechoic stroma. The bulbourethral glands were …

Milk Composition And Yield Changes With Advancing Pregnancy In Dairy Buffaloes (Bubalus Bubalis), Sarzamin Khan, Muhammad Subhan Qureshi, Ifthiar Ahmed, Syed Mirajullah Shah

Turkish Journal of Veterinary & Animal Sciences

The changes in the composition of milk with advancing pregnancy were investigated in Nili-Ravi dairy buffaloes. Forty lactating buffaloes were used, within 2-3 months postpartum. The animals were grouped as high, moderate, and low yielders; synchronized for estrus; and inseminated artificially. Milk yield was recorded daily and sampled fortnightly for analysis. The data were analyzed using the univariate weighted mean and general linear model. Decline in milk yield became significant after the 8th week post-conception. The high yielders had the lowest fat contents. Milk fat increased significantly during succeeding weeks post-conception. The highest fat level (8.5%) was observed in week …

In Vitro Maturation And Fertilization Of Buffalo Oocytes: The Effect Of Recovery And Maturation Methods, Abid Mehmood, Muhammad Anwar, Sayed Murtaza Hassan Andrabi, Muhammad Afzal, Syed Muhammad Saqlan Naqvi

Turkish Journal of Veterinary & Animal Sciences

Conditions for in vitro maturation (IVM) of primary oocytes without conventionally used fetal calf serum and hormones were optimized in order to reduce the cost of laboratory produced buffalo embryos. Comparisons were made between oocyte recovery methods (aspiration vs. slicing) and IVM in medium 199 (static culture method vs. flux culture method) supplemented with 4-5 × 106 granulosa cells mL-1 that contained either estrus buffalo serum (EBS) or fetal calf serum (FCS). Recovery methods were compared according to yield, i.e. cumulus oocyte complexes per ovary (COCs/ovary), the expansion rate (% of COCs that expanded), and nuclear maturation rate (% of …

Computational Comparison Of Β-Mannosidases Of Animals, Humans, Microbes, And Plants, Zahoor Qadir Samra, Muhammad Amin Athar

Turkish Journal of Veterinary & Animal Sciences

The \beta-mannosidase (MANB) enzyme is involved in removing mannose residue from the nonreducing end, and its impaired activity leads to \beta-mannosidosis. MANB amino acid sequences of humans, other mammals, plants, fungi, and bacteria were compared to determine their similarities, differences, and predicted 3D structures. Our cloned MANB DNA sequence showed a 99% similarity to a previously reported human MANB DNA sequence but 16 nucleotide differences were observed, showing the polymorphic nature of the enzyme. The 9 changed codons coded the different amino acids Ile, Lys, Ile, Thr, Arg, Leu, Leu, Gly, and Asp, while 7 changed codons coded the same …

Repeat Breeder Syndrome In Dairy Cows: Influence Of Breed And Age On Its Prevalence And The Success Of A Hormone Therapy*, Robert Zobel, Suzana Tkalcic, Vlatka Buic, Ivana Pipal, Darko Geres, Marko Samardzija

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to establish the prevalence of repeat breeder syndrome (RBS) in different breeds and reproductive ages of cattle in central Croatia and to assess the effectiveness of hormone therapy as a treatment option. A total of 1088 dairy cows were observed, 218 heifers and 870 multiparous cows. After the third unsuccessful artificial insemination (n = 164) and a thorough clinical and gynecological examination, the cows were tested for sexually transmitted diseases. An RBS diagnosis was based on failure to conceive, negative laboratory tests for sexually transmitted diseases, and the absence of other reproductive disorders. The …

Growth Characteristics In Lambs Of Estrus Synchronized Ewes In Grower Conditions, Murat Yilmaz, Tufan Altin

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the growth characteristics of lambs of estrus-synchronized ewes in an extensive production system during 2 different periods. The animal material of this study was composed of 325 head Chios × Kıvırcık cross breeds. These lambs were provided by mating 124 Kıvırcık sheep on 2 farms with 8 Chios rams provided by the Adnan Menderes University, Faculty of Agriculture, Sheep Research Unit. The estrus cycles of the ewes were synchronized with intra-vaginal progestagen sponges impregnated with 30 mg of cronolone. After 12-14 days the sponges were removed, the females received an intramuscular injection …

Expression Of Plasma Prolactin And Pituitary Prolactin Mrna Around The Broody Cycle In Wan-Xi White Goose, Runshen Jiang, Xingyong Chen, Ruhua Wei, Zhaoyu Geng

Turkish Journal of Veterinary & Animal Sciences

A systematic evaluation of the dynamic changes of prolactin (PRL) around the whole cycle of broodiness in domestic fowl has not been performed. We started to investigate the overall changing profile of PRL expression at both protein and mRNA levels from pre- to post-broodiness in Wan-xi White goose, an indigenous breed in East China. The onset of broodiness was recorded, and the plasma PRL concentration and the pituitary PRL mRNA level at 5 stages were measured, in that order, around the first broody cycle. The expression level of pituitary PRL mRNA was highly correlated with the plasma PRL level (r …

Immunodetection Of Insulin-Like Growth Factor Binding Proteins (Igfbps) In The Sera Of Different Animal Species, Romana Masnikosa, Ivona Baricevic, Dragana Lagundzin, Olgica Nedic

Turkish Journal of Veterinary & Animal Sciences

Knowledge regarding the insulin-like growth factor (IGF) system and the methods for the detection and quantitation of IGF binding proteins (IGFBPs) is greater for the human species than for other animal species. This is mainly because antibodies specifically raised against nonhuman IGFBPs are rarely commercially available. We aimed to investigate which of the antibodies directed against human IGFBPs could be used for the detection of IGFBPs present in other species. We used commercially available antibodies, raised against human IGFBP-1 through IGFBP-4, in order to recognize the corresponding IGFBPs in the sera of different animal species: human, bovine, equine, porcine, ovine, …

Gender Differences In Cns Autoimmunity Induced By Mimicry Epitope For Plp 139–151 In Sjl Mice, Chandirasegara Massilamany, Sivasubramani Thulasingam, David Steffen, Jay Reddy

Jay Reddy Publications

Development of multiple sclerosis (MS) is more prevalent in females than in males, but the underlying mechanisms are not clear. Microbial infections have been suspected as triggers of MS and it is not known whether gender differences in reactivity to environmental antigens contribute to the disease pathogenesis. We demonstrated that ACA 83–95, a mimicry epitope from Acanthamoeba castellanii for proteolipid protein (PLP) 139–151, induces clinical signs of encephalomyelitis in both male and female SJL mice. Conversely ACA 83–95-induced effector cells from males fail to induce disease in female mice. Although we found no gender differences in the frequencies of antigen-specific …

Cellular Transcription Factors Induced In Trigeminal Ganglia During Dexamethasone-Induced Reactivation From Latency Stimulate Bovine Herpesvirus 1 Productive Infection And Certain Viral Promoters, Aspen M. Workman, James D. Eudy, Lynette Smith, Devis Sinani, Halie Bricker, Emily Cook, Alan R. Doster, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine herpesvirus 1 (BHV-1), an alphaherpesvirinae subfamily member, establishes latency in sensory neurons. Elevated corticosteroid levels, due to stress, reproducibly triggers reactivation from latency in the field. A single intravenous injection of the synthetic corticosteroid dexamethasone (DEX) to latently infected calves consistently induces reactivation from latency. Lytic cycle viral gene expression is detected in sensory neurons within 6 h after DEX treatment of latently infected calves. These observations suggested that DEX stimulated expression of cellular genes leads to lytic cycle viral gene expression and productive infection. In this study, a commercially available assay—Bovine Gene Chip—was used to compare cellular gene …

Rnai Screening Reveals Requirement For Host Cell Secretory Pathway In Infection By Diverse Families Of Negative-Strand Rna Viruses, Debasis Panda, Anshuman Das, Phat X. Dinh, Sakthivel Subramaniam, Debasis Nayak, Nicholas J. Barrows, James L. Pearson, Jesse Thompson, David L. Kelly, Istvan Ladunga, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

Negative-strand (NS) RNA viruses comprise many pathogens that cause serious diseases in humans and animals. Despite their clinical importance, little is known about the host factors required for their infection. Using vesicular stomatitis virus (VSV), a prototypic NS RNA virus in the family Rhabdoviridae, we conducted a human genomewide siRNA screen and identified 72 host genes required for viral infection. Many of these identified genes were also required for infection by two other NS RNA viruses, the lymphocytic choriomeningitis virus of the Arenaviridae family and human parainfluenza virus type 3 of the Paramyxoviridae family. Genes affecting different stages of …

Y-Like Retinal Ganglion Cells Innervate The Dorsal Raphe Nucleus In The Mongolian Gerbil (Meriones Unguiculatus), Liju Luan, Chaoran Ren, Benson Wui-Man Lau, Jian Yang, Gary E. Pickard, Kwok-Fai So, Mingliang Pu

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: The dorsal raphe nucleus (DRN) of the mesencephalon is a complex multi-functional and multi-transmitter nucleus involved in a wide range of behavioral and physiological processes. The DRN receives a direct input from the retina. However little is known regarding the type of retinal ganglion cell (RGC) that innervates the DRN. We examined morphological characteristics and physiological properties of these DRN projecting ganglion cells.

Methodology/Principal Findings: The Mongolian gerbils are highly visual rodents with a diurnal/crepuscular activity rhythm. It has been widely used as experimental animals of various studies including seasonal affective disorders and depression. Young adult gerbils were used …

Challenges And Opportunities For Toxicology In Mexico, Rodrigo Franco, Balam Muñoz

School of Veterinary and Biomedical Sciences: Faculty Publications

We have developed this Special Issue titled “Challenges and Opportunities for Toxicology in Mexico” with the aim to highlight not only the challenges toxicology research faces in Mexico, but also the efforts and drive towards cutting edge research to address them. Altogether, this special issue should be a valuable source of information for all readers with an interest in toxicology and human health.

Mexico is an important economy worldwide and is a major exporter of several supplies and commodities. Because of this, its industry is a source of occupational exposure to several chemical derivatives (Nieusma 2011). The situation of occupational …

The Need To Quantify Ecosystem Services Provided By Birds, Daniel G. Wenny, Travis L. Devault, Matthew D. Johnson, Dave Kelly, Cagan H. Sekercioglu, Diana F. Tomback, Christopher J. Whelan

United States Department of Agriculture Wildlife Services: Staff Publications

What are the birds worth--what is their actual dollar value to human society? To most of us in the ornithological community, birds are invaluable. But in these times we need more specific rationales to convince policy makers and business leaders to include bird conservation in land-use and development decisions. Over the past two decades, awareness of our dependence on a variety of ecosystem services (natural ecological processes that benefit human society) and of their importance and prevalence has progressed toward the goal of making conservation a mainstream value. Building strategies for the protection of ecosystem services into conservation and land-use …

Molecular And Genomic Approaches To Understanding Host-Virus Interactions In Shaping The Outcome Of Equine Arteritis Virus Infection, Yun Young Go

University of Kentucky Doctoral Dissertations

Equine arteritis virus (EAV) is the causal agent of equine viral arteritis, a disease of equids. During natural outbreaks of the disease, EAV can cause abortion in pregnant mares and persistent infection in stallions. Understanding how host cellular proteins interact with viral RNA and viral proteins, as well as their role in viral infection, will enable better characterization of the pathogenesis of EAV and establishment of persistent infection in stallions. Accordingly, we hypothesized that both viral factors and host genetically related factors could influence the outcome of EAV infection in horses. To test this hypothesis, we first combined contemporary molecular …

Diet Preference And Breeding Success In Captive-Bred Greater Rheas (Rhea Americana): A Preliminary Study, Gisela Bazzano, Joaquin. L. Navarro, Monica. B. Martella

Turkish Journal of Veterinary & Animal Sciences

We conducted 2 consecutive experiments to verify whether adult captive Greater rheas (Rhea americana) could select diets differing in nutritional and energetic content, and to evaluate the effect of those diets on egg production and hatchability. From August to January, animals were offered four diets: processed feed for rheas (diet 1), diet 1 + soybean (diet 2), processed feed for chicken (diet 3), and diet 3 + soybean (diet 4). Rheas preferred the diets containing feed for chicken (diets 3 and 4, 179,67 ± 12.12 [S.E.] g/ind./day). Diet 2 was more consumed (95.67 ± 16.70 g/ind./day) than diet 1 (60.09 …

Association Between Polymorphisms Of Cyp19, Cyp21, And Er1 Genes And Milk Production Traits In Black-And-White Cattle, Magdalena Jedrzejczak, Wilhelm Grzesiak, Iwona Szatkowska, Andrzej Dybus, Magdalena Muszynska, Daniel Zaborski

Turkish Journal of Veterinary & Animal Sciences

The relationships between the SNPs of the cytochrome P450 gene (CYP19/PvuII), steroid 21-hydroxylase (P450c21) gene (CYP21/HpaII), 2 polymorphic sites of estrogen receptor alpha gene (ER1/BglI and ER1/SnaBI), and milk production traits of Black-and-White cattle were analyzed. A total of 472 cows were included in the study and genotyped using PCR-RFLP. The frequencies of alleles for the Black-and-White cows were as follows: 0.923 - CYP19A, 0.077 - CYP19B, 0.042 - ER1/BglIA, and 0.958 for ER1/BglIG. For ER1/SnaBI, the frequency of allele A was 0.960 and that of allele G was 0.040. In the CYP21, all cows were genotyped as AA (no …

Aflatoxin M1 Levels In Retail Yoghurt And Ayran In Erzurum In Turkey, Meryem Aydemi̇r Atasever, Mustafa Atasever, Korhan Özturan

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the presence and levels of aflatoxin M1 (AFM1) in yoghurt and ayran consumed in the province of Erzurum, Turkey. For this purpose, a total of 80 yoghurt samples and 80 ayran samples were randomly obtained from markets. The samples' AFM1 content and concentrations were examined by competitive ELISA method. AFM1 at detectable level (5 ng/kg) was found in 87.5% of the yoghurt samples and in 90.0% of the ayran samples. AFM1 levels in 16 (20%) yoghurt and in 11 (13.6%) ayran samples exceeded the maximum tolerable limit of the Turkish Food Codex. The …

Disodium Edta Used As Anticoagulant Causes Hemolysis In Common Carp Blood, Malgorzata Witeska, Wioleta Wargocka

Turkish Journal of Veterinary & Animal Sciences

Disodium EDTA used as anticoagulant for common carp blood caused a significant increase and high variability in hematocrit readings comparing to the heparinized samples. Na2EDTA induced erythrocyte swelling, causing cell membrane disruption (hemolysis). The nuclei released from destroyed cells changed shape from oval to round, and underwent gradual swelling and vacuolation, followed by karyolysis. The degree of observed changes was similar at all Na2EDTA concentrations (0.1, 0.5, and 1.0 mg/mL); on the other hand, percentage of abnormal and destroyed cells showed considerable differences among blood sampled from various carp individuals. The obtained results revealed that Na2EDTA caused gradual damage to …

Acetic Acid Modulates Induction Of Pulmonary Hypertension In Broiler Chickens: Based On Electrocardiographic Parameters, Hossein Hassanpour, Abdolkarim Zamani Moghadam, Hamed Zarei, Neda Taghavi

Turkish Journal of Veterinary & Animal Sciences

To clarify the effect of acetic acid on the electrocardiographic parameters of pulmonary hypertensive broilers, chicks were reared at high altitude and treated with acetic acid (0.4 and 0.9 g/L) for 45 days. The right ventricle-to-total ventricle ratio was noted as an index of pulmonary hypertension that was significantly (P < 0.05) decreased in the treated groups. S amplitudes were significantly decreased only at 36 days (leads II and III) and 45 days (lead aVF) in many treated groups. There were significant (P < 0.05) reductions of T amplitudes at 28 days (leads aVR and aVL) and 36 days (lead aVL), and significant elevations of QRS (leads II, III, and aVF) and QT intervals (leads II, aVR, aVL, and aVF) at different ages of many treated groups. R amplitudes were significant (P < 0.05) at 28 days (leads aVL and aVF, at both doses of acetic acid), 36 days (leads aVR and aVL, at 0.4 g/L of acetic acid), and 45 days (lead aVR, at both doses of acetic acid). The RR interval also significantly increased at 28 days (leads aVR and aVL) and 45 days (lead aVL). It was concluded that acetic acid modulates induction of ventricular hypertrophy, dilation, and arrhythmia. These effects are detectable in many electrocardiographic parameters.

Massive Toxoplasma Abortions In A Dairy Sheep Flock And Therapeutic Approach With Different Doses Of Sulfadimidine, Nektarios D. Giadinis, Konstantinos Terpsidis, Anastasia Diakou, Victoria Siarkou, Panayiotis Loukopoulos, Ridvan Osman, Harilaos Karatzias`, Margarita Papazahariadou

Turkish Journal of Veterinary & Animal Sciences

In a 500 head dairy sheep flock in Northern Greece, massive abortions (60%) were observed at 110-130 days of gestation. Attempts were made to treat the abortions with oxytetracyclin L.A. 20% but they were not successful. Toxoplasmosis was confirmed by laboratory examination of blood sera of ewes, aborted fetuses, and fetal brain samples. The remaining 200 pregnant sheep were divided in 2 groups of 100 ewes each. Animals of Group 1 were treated with 20 mg/kg b.w. sulfadimidine every other day 4 times, while the animals in Group 2 received 33 mg/kg b.w. sulfadimidine every other day 4 times via …

First Report Of Gtg-Banded Nomenclature Of Pakistani Lohi Sheep (Ovis Aries), Ahmad Ali, Masroor Ellahi Babar, Saqib Mahmood, Muhammad Imran

Turkish Journal of Veterinary & Animal Sciences

The present study was planned to generate the G-band nomenclature of the commonly occurring Lohi sheep breed in the Punjab province of Pakistan. Whole blood samples were collected through jugular venepuncture in heparinised vacutainers. The metaphase slides were prepared using standard protocols. The results from microscopic screening of 50 good quality metaphase cells from each animal were studied and they revealed a chromosome complement of 54, XX and XY in Lohi sheep. The first 3 pairs were sub/metacentric whereas the other autosomes, including X, were classed as acro/subacrocentric. The Y chromosome was the smallest metacentric chromosome and had a star-like …