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Local Anesthetics As Pain Therapy In Horses, Reza Seddighi Dvm, Ms, Phd, Dip Acva

Faculty Publications and Other Works -- Large Animal Clinical Sciences

Local Anesthetics as Pain Therapy in Horses Thomas J. Doherty MVB, MSc, and M. Reza Seddighi DVM, PhD

Department of Large Animal Clinical Sciences, College of Veterinary Medicine, The University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA

This article describes the rationale behind the use of systemically administered lidocaine as an analgesic. The analgesic efficacy of intravenously administered lidocaine is well documented by studies in human patients and laboratory animals. The mechanism by which systemically administered lidocaine produces analgesia is uncertain but is thought to include action at sodium, calcium, and potassium channels and the N-methyl-D-aspartate acid receptor. …

Local Anesthetics As Pain Therapy In Horses, Reza Seddighi Dvm, Ms, Phd, Dip Acva

Reza Seddighi

Local Anesthetics as Pain Therapy in Horses Thomas J. Doherty MVB, MSc, and M. Reza Seddighi DVM, PhD

Department of Large Animal Clinical Sciences, College of Veterinary Medicine, The University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA

This article describes the rationale behind the use of systemically administered lidocaine as an analgesic. The analgesic efficacy of intravenously administered lidocaine is well documented by studies in human patients and laboratory animals. The mechanism by which systemically administered lidocaine produces analgesia is uncertain but is thought to include action at sodium, calcium, and potassium channels and the N-methyl-D-aspartate acid receptor. …

Survey Of Biosecurity Practices Utilized By Veterinarians Working With Farm Animal Species, David E. Anderson

David Edgar Anderson, DVM, MS, DACVS

Biosecurity programs can be discussed from many perspectives. In many cases, biosecurity may be regulated state or federal programs with law enforcement - the goals of which are to protect the "national" livestock resources and protect consumer interests. Alternatively, these programs may be producer level programs that are voluntarily established for protection of personal livestock investments. A core principle in the definition of "biosecurity" is the desire to prevent the introduction of disease. When infectious disease is present on a farm or in a region, the term "biocontainment" may be used to describe programs aimed at confinement of the diseases …

Gene Delivery In The Equine Cornea: A Novel Therapeutic Strategy, Dylan G. Buss, Ajay Sharma, Elizabeth A. Giuliano, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—To determine if hybrid adeno-associated virus serotype 2/5 (AAV5) vector can effectively deliver foreign genes into the equine cornea without causing adverse side effects. The aims of this study were to: (i) evaluate efficacy of AAV5 to deliver therapeutic genes into equine corneal fibroblasts (ECFs) using enhanced green fluorescent protein (EGFP) marker gene and (ii) establish the safety of AAV5 vector for equine corneal gene therapy.

Animal Material—Primary ECF cultures were harvested from healthy donor equine corneas. Cultures were maintained at 370C in humidified atmosphere with 5% CO2.

Procedure—AAV5 vector expressing EGFP under control of hybrid cytomegalovirus (CMV) + chicken …

Isolation And Cultivation Of Equine Corneal Keratocytes, Fibroblasts And Myofibroblasts, Dylan G. Buss, Elizabeth A. Giuliano, Ajay Sharma, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—To establish an in vitro model for the investigation of equine corneal wound healing. To accomplish this goal, a protocol to isolate and culture equine corneal keratocytes, fibroblasts and myofibroblasts was developed.

Animal material—Equine corneal buttons were aseptically harvested from healthy research horses undergoing humane euthanasia for reasons unrelated to this study. Slit-lamp biomicroscopy was performed prior to euthanasia by a board-certified veterinary ophthalmologist to ensure that all samples were harvested from horses free of anterior segment disease.

Procedure—Equine corneal stroma was isolated using mechanical techniques and stromal subsections were then cultured. Customized media at different culture conditions was used …