Veterinary Medicine Commons^™

The Evaluation Of Selected 13 Polymorphic Microsatellite Markers For The Parentage Test Of Thai Holstein Cattle, Lerdchai Chintapitaksakul, Rapiphan Uavechanichkul

The Thai Journal of Veterinary Medicine

Nineteen families of 44 Thai Holstein cattle were evaluated for microsatellite characteristics and the parentage test. A total of 13 microsatellite markers were selected from the genome database and the genomic DNAs were isolated and used as templates for amplification. Multiplex PCR of these thirteen markers in a single reaction showed that only ten markers; b1, b2, b3, b4, b6, b7, b10, b11, b12 and b13 could amplify the fragments ranging from 5 to 12. Nine markers were highly informative with PIC of more than 0.5. An accuracy test revealed that seven markers (b2, b4, b6, b7, b10, b11 and …

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

Effects Of The Renin-Angiotensin Blockade On Renal Functions, Renal Norepinephrine Contents And Oxidative Stress In Cyclosporine Induced Nephrosis Rats, Suvaporn Panasjaroen, Sarinee Kalandakanond-Thongsong, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

The experiment was conducted to investigate the effects of renin-angiotensin blockade on renal function, renal norepinephrine (NE) contents and renal oxidative stress in Cyclosporine A (CsA) induced renal impairment in rats. Rats were assigned into three groups; group 1 (control group), receiving vehicle (propylene glycol) 1 ml/kg./day, subcutaneously (s/c). for 28 days; group 2 (CsA group), receiving CsA 15 mg/kg/day, s/c for 28 days; group 3 (CsA and losartan (LST) group), receiving CsA 15 mg/kg./day, s/c with the administration of LST 10 mg/k.g./day orally for 28 days. The results showed that CsA administration alone elevated mean arterial pressure (MAP), reduced …

Regulation Of The Bioavailability Of Thioredoxin In The Lens By A Specific Thioredoxin-Binding Protein (Tbp-2), Namal P.M. Liyanage, M. Rohan Fernando, Marjorie F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Thioredoxin (TRx) is known to control redox homeostasis in cells. In recent years, a specific TRx binding protein called thioredoxin binding protein-2 (TBP-2) was found in other cell types and it appeared to negatively regulate TRx bioavailability and thereby control TRx biological function. In view of the sensitivity of lens transparency to redox status, proper regulation of TRx bioavailability is of the utmost importance. This study was conducted to examine the presence and function of TBP-2 in human lens epithelial cells (HLE B3). We cloned human lens TBP-2 from a human cDNA library (GenBank accession number AY 594328) and showed …

A Novel Application Of Quantile Regression For Identification Of Biomarkers Exemplified By Equine Cartilage Microarray Data, Liping Huang, Wenying Zhu, Christopher P. Saunders, James N. Macleod, Mai Zhou, Arnold J. Stromberg, Arne C. Bathke

Veterinary Science Faculty Publications

BACKGROUND: Identification of biomarkers among thousands of genes arrayed for disease classification has been the subject of considerable research in recent years. These studies have focused on disease classification, comparing experimental groups of effected to normal patients. Related experiments can be done to identify tissue-restricted biomarkers, genes with a high level of expression in one tissue compared to other tissue types in the body.

RESULTS: In this study, cartilage was compared with ten other body tissues using a two color array experimental design. Thirty-seven probe sets were identified as cartilage biomarkers. Of these, 13 (35%) have existing annotation associated with …

Bovine Viral Diarrhea Virus Multiorgan Infection In Two White-Tailed Deer In Southeastern South Dakota, Christopher C.L. Chase, Lyle J. Braun, Pamela Leslie-Steen, Tanya Graham, Dale Miskimins, Julia F. Ridpath

Veterinary and Biomedical Sciences Faculty Publications

The susceptibility of wild ruminants, especially cervids, to bovine viral diarrhea virus (BVDV) has remained an enigma. Two white-tailed deer (Odocoileus virginianus) were submitted to the Animal Disease Research and Diagnostic Laboratory (ADRDL) in the fall of 2003 by the South Dakota Game Fish and Parks for chronic wasting disease (CWD) testing. Both animals were CWD negative. The animals were necropsied and histopathology, viral antigen detection, and virus isolation were performed. A noncytopathic (NCP) BVDV was isolated from the lungs and several other tissues of both animals. Formalin-fixed ear notches from both animals were positive for BVDV antigen by immunohistochemistry. …

Sheep Updates 2008 - Contents, Department Of Food And Agriculture, Western Australia

Sheep Updates

No abstract provided.

Sheep Updates 2008 - Part 2, Meredith L. Sheil, Di Evans, Brown Besier, Tim Scanlon, Andre Martinho De Almeida, Johan Greeff, Tanya Kilminster, John Milton, Chris Oldham, B. L. Mcintyre, Alison Wheatley, John Lucey, Fiona Jones, Nick Costa, Ed Riggall, J. R. L. Hall, Robin Jacob, Dave Pethick, Dave Hopkins, Graham Gardner

Sheep Updates

This session covers eleven papers from different authors: The Sheep Room 1. Analgesia for Surgical Husbandry Procedures in Sheep and Other Livestock, Dr Meredith L. Sheil, Animal Ethics Pty Ltd, Associate Sydney University Faculty of Veterinary Science The Wool Enterprise 2. Unmulsed sheep - implications for chemical use, Di Evans & Brown Besier, Department of Agriculture and Food WA 3. Are Damara and Dorper sheep better adapted than Merinos to nutritional stress? - Growth rates, Tim Scanlon1, Andre Martinho de Almeida2, Johan Greeff1, Tanya Kilminster1, John Milton3, Chris Oldham1, Department of Agriculture and Food WA1, Instituto de Investigacao Cientifica Tropical, …

Sheep Updates 2008 - Part 1, David Hughes, Tony Higgs, Di Evans, Scott Hansen, Michael Paton, Mary Nenke

Sheep Updates

This session covers five papers from different authors: KEYNOTE 1. Global trends in consumer and community expectations and their affect on the market, Dr David Hughes, Imperial College, London. (Powerpoint) PLENARY 2. Animal welfare - trends and opportunities, Tony Higgs, Di Evans, Department of Agriculture and Food WA 3. Australia's live sheep exports to Saudi Arabia, Scott Hansen, Meat & Livestock Australia 4. Livestock Welfare Challenges in Road Transport, Dr Mike Paton, Department of Agriculture and Food, Western Australia 5. My 2020 Summit Experience, Mary Nenke, Producer Kukerin WA

Comparison Of The Contributions Of Heat-Labile Enterotoxin And Heat-Stable Enterotoxin B To The Virulence Of Enterotoxigenic Escherichia Coli In F4ac Receptor-Positive Young Pigs, Joseph Erume, Emil M. Berberov, Stephen D. Kachman, Michael A. Scott, You Zhou, David H. Francis, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

In swine, the most common and severe enterotoxigenic Escherichia coli (ETEC) infections are caused by strains that express K88 (F4)⁺ fimbriae, heat-labile enterotoxin (LT), heat-stable enterotoxin b (STb), and enteroaggregative E. coli heat-stable toxin 1. Previous studies based on a design that involved enterotoxin genes cloned into a nontoxigenic fimbriated strain have suggested that LT but not STb plays an important role in dehydrating diarrheal disease in piglets study, we compared these two toxins in terms of importance for piglets >1 week old with a study design that involved construction of isogenic single- and double-deletion mutants and inoculation of …

Assessment Of Risk Associated With The Minnesota Proposed Plan For Split-State Status For Mycobacterium Bovis (Bovine Tuberculosis)

Michigan Bovine Tuberculosis Bibliography and Database

In July 2005, a Minnesota beef herd tested positive for Mycobacterium bovis (M. bovis) and was officially declared infected with bovine tuberculosis (TB). This was the first infected herd identified since Minnesota’s Accredited Free (AF) status was obtained in 1976. Subsequent testing identified infection in white-tailed deer and several cattle herds adjacent to this index herd. In February 2008, Minnesota declared its 11th infected cattle herd, resulting in the downgrade of the entire State’s TB status to Modified Accredited (MA).
In an effort to minimize the impact of MA status to the State and producers, Minnesota initiated the …

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director's Message [Page] 1- Partnerships Matter Diagnostic News [Page] 1- Bovine Lead Poisoning: May and June are Peak Months for Diagnosis [Page] 3- Holiday Hours [Page] 3- Water Suitability for Livestock [Page] 4- Why can’t you just run it anyway? Research News [Page] 4- Research Spotlight: Dr. Chris Chase Extension News [Page] 5- State Veterinary Loan Repayment Programs –a Regional Review [Page] 6- Pieces and Parts Student News [Page] 7- SDSU Pre-Veterinary Students Admitted to Veterinary Colleges for Fall 2008 [Page] 7- 2008 – 2009 Vet Science Scholarship Award Recipients [Page] 7- Calendar of Events

Renal Function And Oxidative Stress Following Gentamicin Induced Renal Injury In Rats Treated With Erythropoietin, Iron And Vitamin E, Patcharin Thongchai, Narongsak Chaiyabutr, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

The effects of erythropoietin(Epo), iron, and vitamin E on renal function and oxidative stress in rats with renal injury induced by gentamicin were investigated. Rats were divided into 5 groups; group 1, control; group 2, gentamicin group(100 mg/kg gentamicin sc. on days 5-12); group 3, gentamicin plus Epo (in addition to gentamicin, 100 i.u./kg Epo was administered s.c. on days 5-12); group 4, gentamicin plus Epo and iron (in addition to gentamicin and Epo, 500 mg/kg iron was administered i.p. on days 4) and group 5, gentamicin plus Epo, iron, and vitamin E (in addition to genta, Epo and iron, …

The Microscopic And Molecular Detections Of Canine Ehrlichiosis, Orapin Ariyawutthiphan, Kaj Shokshai-Utsaha, Thanida Sananmuang, Sudchit Chungpivat, Meena Sarikaputi, Nareerat Viseshakul

The Thai Journal of Veterinary Medicine

The applications of molecular detection using nested PCR and the routine microscopic examination of ehrlichiosis were applied to 43 clinically affected dogs residing in Bangkok and Samutraprakarn. This study, performed in 2005-2006, has determined that 79.5% (31/39) of the buffy coat smears were having the ehrlichial morulae plus initial bodies found in monocytes and lymphocytes. The nested PCR was used to confirm the occurrence of ehrlichiosis after tested by morulae identification. Both tests demonstrated that neither Ehrlichia chaffeensis nor E. ewingii, the zoonotic agents of human, were found in this area. However, the prevalence of E. canis alone detected by …

Distribution Of Edwardsiella Tarda Antigens And Igm Containing Cells In Tilapia Immune Organs During Septicemia: An Immunohistochemical Study, Nopadon Pirarat, Takayuki Katagiri, Masashi Maita, Makoto Endo, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

An immunohistochemical study describes the localization of IgM containing cells involved in the primary immune organs of tilapia and the fate of E. tarda antigen distributed in the tissues during a course of intraperitoneal challenge. The E. tarda antigen was diffusely detected in various organs except the brain and spinal cord. The positive signals were identified within various cell types, including phagocytic cells, serosal mesothelia, myocardia, intestinal and gill epithelia and glomerular capillary endothelia. No extracellular deposition of bacterial antigen was observed. The bacterial antigen can persist up to 1 month in the granuloma and melano-macrophage center, confirming the intracellular …

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

Known Facts About The Epidemiology Of Porcine Proliferative Enteropathy, Roberto M. C. Guedes

The Thai Journal of Veterinary Medicine

The amount of new information of the last seven years regarding Lawsonia intracellularis sensitivity to antimicrobial drugs, prevention/control based on treatment and vaccination, diagnosis and, finally, genome is amazing. Nevertheless, not much is known about the epidemiology of the disease, mainly regarding the source of infection, resistance of the bacteria in the environment and possible biological vectors that could spread the infection among farms. It was learned that as few as 1,000 to 10,000 bacteria are enough to induce infection and fecal shedding two to three weeks after inoculating susceptible pigs. It was also learned that L. intracellularis organisms can …

Tube Realignment For Patellar Luxation Repair In Dogs, Chalika Wangdee, Marissak Kalpravidh

The Thai Journal of Veterinary Medicine

Grade II patellar luxations in dogs were repaired by trochlear wedge recession in 5 stifles (group 1) and by proximal tube realignment in 5 stifles (group 2). A combination of the two techniques was used to correct grade II luxation in 10 stifles (group 3) and grade III luxation in 5 stifles (group 4). The position of the patella, weight bearing, and animal gait were postoperatively examined for 12 weeks. Satisfactory results were observed in 1(20%), 3(60%), and 9(90%) stifles with grade II luxation in groups 1, 2, and 3, respectively. Good recovery from grade III luxation was observed in …

Low Pregnancy Rate In Dairy Cattle After Fixed Time Artificial Insemination Using Norgestomet + Pgf2Α + Ecg Program During The Hot And Humid Months In Thailand, Winai Kaewlamun, Junpen Suwimonteerabutr, Thitirat Chaimee, Prachin Virakul, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The present study was conducted to synchronize estrus and fixed time insemination in dairy cattle during the hot and humid months (July to November) in Thailand. Thirty-four cows and heifers were treated by using norgestomet+PGF2α+equine chorionic gonadotropin (eCG) program. Norgestomet was implanted subcutaneously at the back of the ear for 10 days. PGF2α was administered IM on day 8 and one injection of eCG was given on the day of implant removal. Fixed time artificial insemination(AI) were performed twice, at about 54-60 and 70-74 h, after the eCG injection. Blood samples were collected at 22 days after AI for progesterone …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

The Incidence On Severe Losses Of Piglets In Central Thailand Caused By The Ped Viral Disease, Supol Luengyosleuchakul

The Thai Journal of Veterinary Medicine

No abstract provided.

Recombinant Mycobacteria Overexpressing D-Alanine Ligase Gene And Uses Therefore: United States Patent No. Us 7,371,571 B2, Raul G. Barletta, Zhengyu Feng

School of Veterinary and Biomedical Sciences: Faculty Publications

Recombinant mycobacterial strains which overproduce essential biosynthetic enzymes of pathogenic mycobateria are provided. These strains overproduce enzymes involved in the synthesis and incorporation of D-alanine into mycobacterial peptidoglycan, the backbone of the mycobacterial cell wall. These overproducing strains may be used as reference strains in in vitro screening methods to identify antimycobacterial agents.

Transmission Of Panulirus Argus Virus 1 (Pav1) And Its Effect On The Survival Of Juvenile Caribbean Spiny Lobster, Mark J. Butler Iv, Donald C. Behringer Jr., Jeffrey D. Shields

Biological Sciences Faculty Publications

The Caribbean spiny lobster Panulirus argus, an important fisheries species, is host to Panulirus argus virus 1 (PaV1), a lethal, unclassified virus-the first found in any species of lobster-prevalent in juvenile lobsters. We describe a series of laboratory experiments aimed at assessing the likely modes of disease transmission, determining the survival of lobsters relative to each transmission pathway and identifying potential alternate hosts. Given evidence for lower prevalence of PaV1 in large lobsters, the effect of lobster size on susceptibility was also examined. Results demonstrated that PaV1 can be transmitted to juvenile lobsters via inoculation, ingestion of diseased tissue, contact …

Tetanus And Rabies Eradication In Pakistan; A Mission Not Impossible, Mohammad Wasay, Ismail A. Khatri, Naseem Salahuddin

Department of Medicine

No abstract provided.

Antibiotic-Mediated Inhibition Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Infection: A Novel Quinolone Function Which Potentiates The Antiviral Cytokine Response In Marc-145 Cells And Pig Macrophages, William A. Cafruny, Richard G. Duman, Raymond R. Rowland, Eric A. Nelson, Grace H. Wong

Veterinary and Biomedical Sciences Faculty Publications

Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically significant agent for which there currently are no effective treatments. Development of antiviral agents for PRRSV as well as many other viruses has been limited by toxicity of known antiviral compounds. In contrast, antibiotics for non-virus microbial infections have been widely useful, in part because of their acceptable toxicity in animals. We report here the discovery that the quinolone containing compound Plasmocin™, as well as the quinolones nalidixic acid and ciprofl oxacin, have potent anti-PRRSV activity in vitro. PRRSV replication was inhibited by these antibiotics in both cultured MARC-145 cells …

Effects Of The Addition Of Probiotic Containing Both Bacteria And Yeast Or An Antibiotic On Performance Parameters, Mortality Rate And Antibiotic Residue In Broilers, Boonrit Thongsong, Sarinee Kalandakanond-Thongsong, Vivat Chavananikul

The Thai Journal of Veterinary Medicine

The experiment was conducted to investigate the effects of supplementing a commercially available probiotic (an Active Elements®; AE) containing Lactobacillus plantarum and Saccharomyces cerevisiae, an antibiotic; Chlortetracycline (CTC), and the probiotic plus antibiotic, via the drinking water on broiler production performance, mortality rate and the antibiotic residue in meat. Four hundred one-day-old male Cobb broiler chicks were randomly allocated to 4 treatments; a non-treated control, a 0.05% CTC, a 1:500 AE, and a combination of CTC and AE. Chicks were fed with a commercial broiler diet ad libitum. They were reared under an environmentally controlled house for 6 weeks. The …

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

Note From The Advisory Committee, Annop Kunavongkrit

The Thai Journal of Veterinary Medicine

No abstract provided.