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2002

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Articles 61 - 90 of 305

Full-Text Articles in Veterinary Medicine

Susceptibility Of Raccoons (Procyon Lotor) To Infection With Mycobacterium Bovis, Mitchell V. Palmer, W. Ray Waters, Diana L. Whipple Jan 2002

Susceptibility Of Raccoons (Procyon Lotor) To Infection With Mycobacterium Bovis, Mitchell V. Palmer, W. Ray Waters, Diana L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

Tuberculosis due to Mycobacterium bovis infection is endemic in white-tailed deer (Odocoileus virginianus) in the northeastern portion of the lower Michigan peninsula (USA). Various wild carnivores and omnivores, including raccoons (Procyon lotor), are infected with M. bovis within the endemic area. To investigate the pathogenesis of tuberculosis in raccoons and the likelihood of M. bovis transmission from infected raccoons to other susceptible hosts, we experimentally inoculated raccoons with single oral doses of M. bovis (ranging from 30 to 1.7 x 105 colony forming units [CFU]), five daily oral doses of M. bovis (ranging from 10 …

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Milk Containing Mycobacterium Bovis As A Source Of Infection For White-Tailed Deer Fawns (Odocoileus Virginianus), M. V. Palmer, W. R. Waters, D. L. Whipple Jan 2002

Milk Containing Mycobacterium Bovis As A Source Of Infection For White-Tailed Deer Fawns (Odocoileus Virginianus), M. V. Palmer, W. R. Waters, D. L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

Setting:White-tailed deer represent the first wildlife reservoir of Mycobacterium bovis in the United States. The behavior of does with nursing fawns provides several potential mechanisms for disease transmission. Little information exists concerning transmission between doe and fawn, specifically transmammary transmission.

Objective: Determine if fawns can become infected by ingestion of milk replacer containing M. bovis, thus simulating transmission from doe to fawn through contaminated milk.

Design: Seventeen, 21-day-old white-tailed deer fawns were inoculated orally with 2x108 CFU (high dose, n=5), 2.5 x 105 to 2.5 x 106 CFU (medium dose, n=5), and 1x104 CFU (low …

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Mechanisms Counteracting Swelling In Brain Cells During Hyponatremia, Herminia Pasantes-Morales, Rodrigo Franco, Benito Ordaz, Lenin D. Ochoa Jan 2002

Mechanisms Counteracting Swelling In Brain Cells During Hyponatremia, Herminia Pasantes-Morales, Rodrigo Franco, Benito Ordaz, Lenin D. Ochoa

School of Veterinary and Biomedical Sciences: Faculty Publications

Water gain in the brain consequent to hyponatremia is counteracted by mechanisms that initially include a compensatory displacement of liquid from the interstitial space to cerebrospinal fluid and systemic circulation and subsequently an active reduction in cell water accomplished by extrusion of intracellular osmolytes to reach osmotic equilibrium. Potassium (K+), chloride (Cl–), amino acids, polyalcohols, and methylamines all contribute to volume regulation, with a major contribution of ions at the early phase and of organic osmolytes at the late phase of the regulatory process. Experimental models in vitro show that osmolyte fluxes occur via leak pathways …

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Dynamics Of Bovine Tuberculosis In Wild White-Tailed Deer In Michigan, Graham J. Hickling Jan 2002

Dynamics Of Bovine Tuberculosis In Wild White-Tailed Deer In Michigan, Graham J. Hickling

Michigan Bovine Tuberculosis Bibliography and Database

In mid-2001, the author of this report was invited to undertake an exploratory analysis of Michigan Department of Natural Resources’ data on bovine tuberculosis (TB) infection in wild white-tailed (Odocoileus virginianus) in the northeastern Lower Peninsula of the state. The aims of this analysis were:
• to quantify the geographic spread of tuberculous deer in Michigan;
• to investigate key factors influencing the prevalence of disease in the core of the infected area;
• to interpret the results in light of overseas experience with wildlife TB, with a view to
recommending how current management of the disease might …

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Environmental And Farm Management Factors Associated With Tuberculosis On Cattle Farms In Northeastern Michigan, John B. Kaneene, Colleen S. Bruning-Fann, Larry M. Granger, Roseann Miller, Barbara A. Porter-Spalding Jan 2002

Environmental And Farm Management Factors Associated With Tuberculosis On Cattle Farms In Northeastern Michigan, John B. Kaneene, Colleen S. Bruning-Fann, Larry M. Granger, Roseann Miller, Barbara A. Porter-Spalding

Michigan Bovine Tuberculosis Bibliography and Database

Objective—To identify major environmental and farm management factors associated with the occurrence of tuberculosis (TB) on cattle farms in northeastern Michigan.

Design—Case-control study.

Sample Population—17 cattle farms with infected cattle and 51 control farms.

Procedure—Each case farm (laboratory confirmed diagnosis of Mycobacterium bovis infection) was matched with 2 to 4 control farms (negative whole-herd test results within previous 12 months) on the basis of type of farm (dairy or beef) and location. Cattle farm data were collected from in-person interviews and mailed questionnaires. Wildlife TB data were gathered through state wildlife surveillance. Environmental data were gathered …

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Tonsillar Lesions In White-Tailed Deer (Odocoileus Virginianus) Naturally Infected With Mycobacterium Bovis, M. V. Palmer, D. L. Whipple, K. L. Butler, S. D. Fitzgerald, C. S. Bruning-Fann, S. M. Schmitt Jan 2002

Tonsillar Lesions In White-Tailed Deer (Odocoileus Virginianus) Naturally Infected With Mycobacterium Bovis, M. V. Palmer, D. L. Whipple, K. L. Butler, S. D. Fitzgerald, C. S. Bruning-Fann, S. M. Schmitt

Michigan Bovine Tuberculosis Bibliography and Database

In 1994, a free-living white-tailed deer (Odocoileus virginianus) in Michigan was diagnosed with tuberculosis caused by Mycobacterium bovis (Schmitt and others 1997). Subsequent surveys conducted by the Michigan Department of Natural Resources and Michigan State University Animal Health Diagnostic Laboratory identified an epidemic of M bovis infection in free-living white-tailed deer in northeast Michigan (Schmitt and others 1997, O'Brien and others 2001). This represents the first known reservoir of M bovis in free-living wildlife in the USA, and the first known epidemic of tuberculosis in white-tailed deer anywhere in the world.

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Lesion Development In White-Tailed Deer (Odocoileus Virginianus) Experimentally Infected With Mycobacterium Bovis, M. V. Palmer, W. R. Waters, D. L. Whipple Jan 2002

Lesion Development In White-Tailed Deer (Odocoileus Virginianus) Experimentally Infected With Mycobacterium Bovis, M. V. Palmer, W. R. Waters, D. L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

The recent discovery of tuberculosis in free-living white-tailed deer in northeastern Michigan underscores the need for increased understanding of the pathogenesis of tuberculosis in wildlife species. To investigate lesion development in white-tailed deer, 32 deer were experimentally infected by intratonsilar instillation of 300 colony-forming units of Mycobacterium bovis. Three deer each were euthanatized and examined at days 15, 28, 42, and 56 after inoculation, and five deer each were euthanatized and examined at days 89, 180, 262, and 328 after inoculation. Microscopic lesions first were seen in the medial retropharyngeal lymph node and lung 28 and 42 days after …

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Mycobacterium Bovis–Infected White-Tailed Deer (Odocoileus Virginianus): Detection Of Immunoglobulin Specific To Crude Mycobacterial Antigens By Elisa, W. Ray Waters, Mitchell V. Palmer, Diana L. Whipple Jan 2002

Mycobacterium Bovis–Infected White-Tailed Deer (Odocoileus Virginianus): Detection Of Immunoglobulin Specific To Crude Mycobacterial Antigens By Elisa, W. Ray Waters, Mitchell V. Palmer, Diana L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

White-tailed deer (Odocoileus virginianus) have recently emerged as a source of Mycobacterium bovis infection for cattle within North America. The objective of this study was to evaluate the antibody response of M. bovis–infected deer to crude mycobacterial antigens. Deer were experimentally inoculated with M. bovis strain 1315 either by intratonsilar instillation or by exposure to M. bovis–infected (i.e., in contact) deer. To determine the time course of the response, including the effects of antigen administration for comparative cervical skin testing, serum was collected periodically and evaluated by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin (i.e., IgG heavy …

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Nitric Oxide Production As An Indication Of Mycobacterium Bovis Infection In White-Tailed Deer (Odocoileus Virginianus), W. R. Waters, M. V. Palmer, R. E. Sacco, D. L. Whipple Jan 2002

Nitric Oxide Production As An Indication Of Mycobacterium Bovis Infection In White-Tailed Deer (Odocoileus Virginianus), W. R. Waters, M. V. Palmer, R. E. Sacco, D. L. Whipple

Michigan Bovine Tuberculosis Bibliography and Database

White-tailed deer ( Odocoileus virginianus ) are reservoirs for Mycobacterium bovis in northeast Michigan, USA. Production of nitric oxide (NO) by activated macrophages is a potent mechanism of mycobacterial killing. The capacity of macrophages to produce NO, however, varies among mammalian species. The objective of this study was to determine if mononuclear cells from white-tailed deer produce nitrite as an indication of NO production and, if so, is NO produced in response to stimulation with M. bovis antigens. Supernatants were harvested from adherent peripheral blood mononuclear cell (PBMC) cultures that had been stimulated with either Mannheimia haemolytica lipopolysaccharide (LPS) or …

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Bovine Tuberculosis In Michigan Wildlife And Livestock, Stephen M. Schmitt, Daniel J. O'Brien, Colleen S. Brunning-Fann, Scott D. Fitzgerald Jan 2002

Bovine Tuberculosis In Michigan Wildlife And Livestock, Stephen M. Schmitt, Daniel J. O'Brien, Colleen S. Brunning-Fann, Scott D. Fitzgerald

Michigan Bovine Tuberculosis Bibliography and Database

Since 1994, the state of Michigan has recognized a problem with bovine tuberculosis (TB), caused by Mycobacterium bovis, in wild white-tailed deer from a 12-county area in northeastern Lower Michigan. A total of 65,000 free-ranging deer have been tested, and 340 have been found to be positive for M. bovis. The disease has been found in other wildlife species, and, in 1998, in domestic cattle, where to date 13 beef cattle and 2 dairy cattle herds have been diagnosed with bovine TB. Unfortunately, the situation is unique in that there have never been reports of self-sustaining bovine TB …

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Mycobacterium Smegmatis D-Alanine Racemase Mutants Are Not Dependent On D-Alanine For Growth, Ofelia Chacon, Zhengyu Feng, N. Beth Harris , Nancy E. Caceres, Garry Adams, Raul G. Barletta Jan 2002

Mycobacterium Smegmatis D-Alanine Racemase Mutants Are Not Dependent On D-Alanine For Growth, Ofelia Chacon, Zhengyu Feng, N. Beth Harris , Nancy E. Caceres, Garry Adams, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium smegmatis is a fast-growing nonpathogenic species particularly useful in studying basic cellular processes of relevance to pathogenic mycobacteria. This study focused on the D-alanine racemase gene (alrA), which is involved in the synthesis of D-alanine, a basic component of peptidoglycan that forms the backbone of the cell wall. M. smegmatis alrA null mutants were generated by homologous recombination using a kanamycin resistance marker for insertional inactivation. Mutants were selected on Middlebrook medium supplemented with 50 mM D-alanine and 20 μg of kanamycin per ml. These mutants were also able to grow in standard and minimal media without …

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Misconceptions Surrounding The Safety Of Home Birth And Hospital Birth, Misty D. Richard Jan 2002

Misconceptions Surrounding The Safety Of Home Birth And Hospital Birth, Misty D. Richard

LSU Master's Theses

Much debate surrounds the topic of women choosing to deliver their infants at home with the services of a midwife. The outcomes of women beginning labor at home, ultimately delivering at home, and the infants born at home were studied in order to determine the safety of home birth among expectant women choosing to be attended by a midwife licensed to practice by the state of Louisiana. Trends associated with those choosing home birth and the frequency of home birth were also analyzed. The study was conducted using retrospective records of clients accepted during the study years 1986-2000. During the …

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The Effect Of Experimental Gastric Dilatation-Volvulus On The Adenosine Triphosphate Content And Cellular Permeability Of The Canine Gastric And Jejunal Mucosa, Laura Elizabeth Peycke Jan 2002

The Effect Of Experimental Gastric Dilatation-Volvulus On The Adenosine Triphosphate Content And Cellular Permeability Of The Canine Gastric And Jejunal Mucosa, Laura Elizabeth Peycke

LSU Master's Theses

This study was designed to characterize changes in the content of adenosine triphosphate (ATP) and cellular permeability of gastric and jejunal mucosa during gastric dilatation-volvulus (GDV). Our hypothesis stated that experimental GDV would result in a decrease in ATP content and cellular integrity of the gastric and intestinal mucosa that may improve after decompression and derotation of the stomach. Fifteen medium-to-large mixed-breed male dogs were randomly divided into three groups: GDV dogs, Ischemia dogs and Control dogs. All dogs were maintained on gas anesthesia for 210 minutes. The GDV dogs had a GDV for 120 minutes with a 90-minute period …

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Antipredator Conditioning In Mississippi Sandhill Cranes (Grus Canadensis Pulla), Jennifer Jill Heatley Jan 2002

Antipredator Conditioning In Mississippi Sandhill Cranes (Grus Canadensis Pulla), Jennifer Jill Heatley

LSU Master's Theses

The Mississippi Sandhill Crane (Grus canadensis pulla), the most endangered North American crane, is considered critically endangered and is protected by Federal and State law. Substantial funding has established the Mississippi Sandhill Crane National Wildlife Refuge in Southern Mississippi and an artificial insemination / breeding facility at the Audubon Center for Research of Endangered Species in New Orleans, Louisiana to promote species recovery. In spite of extensive time, labor, and money invested in captive propagation, juvenile Mississippi Sandhill Cranes suffer substantial mortality due to predation by bobcats (Lynx rufus), coyotes (Canis latrans) and red tailed hawks (Buteo jamaicensis) upon reintroduction …

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Maximising The Lamb Drop, Don Moir Jan 2002

Maximising The Lamb Drop, Don Moir

Bulletins 4000 -

Although reproductive rates vary greatly between individual properties, the average marking percentage in Western Australia still hovers around 75%. In the absence of disease it is possible to improve on this figure by undertaking some straight forward management practices at different stages of the reproductive cycle.

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Evidence For Infanticide In Bottlenose Dolphins Of The Western North Atlantic, Dale G. Dunn, Susan G. Barco, D. Ann Pabst, William A. Mclellan Jan 2002

Evidence For Infanticide In Bottlenose Dolphins Of The Western North Atlantic, Dale G. Dunn, Susan G. Barco, D. Ann Pabst, William A. Mclellan

Biological Sciences Faculty Publications

Nine bottlenose dolphin (Tursiops truncatus) calves that stranded in Virginia in 1996 and 1997 died of severe blunt-force trauma. Injuries were concentrated on the head and chest and multiple rib fractures, lung lacerations, and soft tissue contusions were prominent. Skeletal and/or soft tissue trauma occurred bilaterally in all of the calves. One had a bite wound across the left mandible that exhibited deep punctures consistent with the tooth placement in an adult bottlenose dolphin. The lesions were not compatible with predation, boat strike, fisheries interactions, rough-surf injury, or blast injury. However, they were similar to traumatic injuries described in stranded …

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Influence Of Protein Tyrosine Kinases On Cell Volume Change-Induced Taurine Release, Herminia Pasantes-Morales, Rodrigo Franco Jan 2002

Influence Of Protein Tyrosine Kinases On Cell Volume Change-Induced Taurine Release, Herminia Pasantes-Morales, Rodrigo Franco

School of Veterinary and Biomedical Sciences: Faculty Publications

Taurine efflux occurs in association with cell swelling in both hyposmotic and isosmotic conditions and during cell shrinkage in apoptotic death. Release occurs through a leak pathway, is largely Ca2+-independent and is sensitive to Cl– channel blockers. Taurine efflux elicited by hyposmolarity is reduced or suppressed by tyrosine kinase blockers and increased by tyrosine phosphatase inhibitors. The specific kinases involved are still unknown and may be different in the various cell types. Non-receptor and scr-related protein kinases have been identified in some cells as elements that directly phosphorylate the taurine efflux pathway. Possible tyrosine kinase targets are …

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Methods And Compositions For Protection Against Bovine Viral Diseases, Subramaniam Srikumaran Jan 2002

Methods And Compositions For Protection Against Bovine Viral Diseases, Subramaniam Srikumaran

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention relates to methods and compositions for eliciting an immune response against bovine viral epitopes. The methods comprise combining at least one heat shock protein with at least one bovine viral epitope to form a purified epitope/heat shock protein complex and administration of an immune system stimulating amount of the purified epitope/heat shock protein complex. The compositions comprise, a purified epitope/heat shock protein complex comprising at least one bovine viral epitope complexed with at least one heat shock protein, and a pharmaceutically acceptable carrier, diluent or excipient.

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Parelaphostrongylus Tenuis In Captive Pronghorn Antelope (Antilocapra Americana) In Nebraska, Heather A. Simmons, David J. Steffen, Douglas L. Armstrong, Douglas G. Rogers Jan 2002

Parelaphostrongylus Tenuis In Captive Pronghorn Antelope (Antilocapra Americana) In Nebraska, Heather A. Simmons, David J. Steffen, Douglas L. Armstrong, Douglas G. Rogers

School of Veterinary and Biomedical Sciences: Faculty Publications

Lesions in four captive pronghorn antelope (Antilocapra americana) naturally infected with Parelaphostrongylus tenuis in eastern Nebraska (USA) are described in this report. Animals were bright and alert with hind limb ataxia that progressed to sternal or lateral recumbency between July 28 and October 17, 1998. Animals were euthanized due to disease progression despite therapy. Multifocal decubital ulcers over bony prominences occurred in two animals and chronic unilateral otitis media was present in one animal. Histopathologic examination revealed severe Wallerian degeneration randomly scattered throughout the spinal cords of all four animals. Spinal cord sections from two animals contained adult …

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Pathogenic Mechanisms Of Photobacterium Damselae Subspecies Piscicida In Hybrid Striped Bass, Ahmad A. Elkamel Jan 2002

Pathogenic Mechanisms Of Photobacterium Damselae Subspecies Piscicida In Hybrid Striped Bass, Ahmad A. Elkamel

LSU Doctoral Dissertations

Photobacterium damselae subspecies piscicida, previously known as Pasteurella piscicida, is an important pathogen of hybrid striped bass and many fish species cultured in brackish water in the United States, Japan, Europe, and the Mediterranean. The purpose of this study is to investigate virulence mechanisms that contribute to the pathogenesis of this organism. The ability of P. damselae to survive/replicate within hybrid striped bass macrophages was evaluated with an in vitro killing assay. Results indicated that the numbers of bacteria recovered from macrophages at 3, 6, 12, and 18 hours of incubation increased significantly over time. In contrast, the numbers of …

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Plasmid-Mediated Expression Of Foreign Genes In Ehrlichia Canis, Langston Dolphus Hull Jan 2002

Plasmid-Mediated Expression Of Foreign Genes In Ehrlichia Canis, Langston Dolphus Hull

LSU Doctoral Dissertations

Ehrlichia canis is the etiologic agent of “tropical canine pancytopenia”, or canine ehrlichiosis. The impetus for this research was to overcome the lack of any reliable means of elucidating the genetic profiles of these illusive and historically difficult to manipulate organisms. The use of a broad-host range plasmid greatly facilitated the determination of an electro-transformation protocol. The transforming plasmid possesses a chloramphenicol antibiotic resistance gene marker (chloramphenicol acetyltransferase [CAT] gene), and a visual reporter gene marker, the Green Fluorescent Protein (GFP) gene. With primer sets designed to specifically amplify these two plasmid encoded gene markers, thus verifying the presence of …

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Effects Of Selenium On Thyroid Hormone Levels In Sheep, Ahmet Ateşşahi̇n, İbrahi̇m Pi̇ri̇nçci̇, Feri̇t Gürsu, Gürkan Çikim Jan 2002

Effects Of Selenium On Thyroid Hormone Levels In Sheep, Ahmet Ateşşahi̇n, İbrahi̇m Pi̇ri̇nçci̇, Feri̇t Gürsu, Gürkan Çikim

Turkish Journal of Veterinary & Animal Sciences

In this study, the thyroid hormone levels in sheep experimentally treated with selenium were investigated. Twelve Akkaraman sheep were used in the investigation. Selenium was given intramuscularly at doses of 0.1 (Group 1) and 0.4 mg/kg (Group 2). The blood samples were taken from the jugular vein at 1, 2, 4, 8, 24, 48, 96 and 144 hours after the administration. Serum triiodotyronine (T3), thyroxin (T4), free triiodotironine (fT3) and free thyroxin (fT4) levels were determined using a hormone autoanalyzer. In the comparison between the groups, it was shown that serum T3 levels reached the maximum levels at the 96^{th} …

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The Relationships Between Colourfullness And Physical Properties Of Down And Coarse Fibres Obtained From Goats Raised In Turkey, Feryal Söylemezoğlu, Zeynep Erdoğan, Gürsel Dellal, Ali̇ Murat Tatar Jan 2002

The Relationships Between Colourfullness And Physical Properties Of Down And Coarse Fibres Obtained From Goats Raised In Turkey, Feryal Söylemezoğlu, Zeynep Erdoğan, Gürsel Dellal, Ali̇ Murat Tatar

Turkish Journal of Veterinary & Animal Sciences

In this research, the colourfullness and the relationships between colourfullness and physical characteristics in the coarse and down fibres obtained from hair goats raised in the provinces of Antalya, Artvin, Diyarbakır, İzmir, Kars and Konya in Turkey were studied. It was found that the most common colours were black (69%), white (9%) and milky brown (37%), and brown (15%) and white (14%), respectively in the coarse and down fibres for the provinces overall. The difference in fineness between the white and cream colour groups was significant (p

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Use Of Milk Progesterone Assay And Rectal Palpation To Monitor Postpartum Anoestrus And The Effect Of Prid-Pmsg Treatment In Murrah Buffaloes, Mehmet Uçar, Mustafa Küçükkebapçi, Muzaffer Çelebi̇, Havva Neşe Akalin Jan 2002

Use Of Milk Progesterone Assay And Rectal Palpation To Monitor Postpartum Anoestrus And The Effect Of Prid-Pmsg Treatment In Murrah Buffaloes, Mehmet Uçar, Mustafa Küçükkebapçi, Muzaffer Çelebi̇, Havva Neşe Akalin

Turkish Journal of Veterinary & Animal Sciences

Postpartum anoestrus is widely known to cause infertility in buffaloes. This study was conducted to determine the problem of postpartum anoestrus using rectal palpation and milk progesterone level in 8 Murrah buffaloes calved during late spring or early summer in the Kocatepe Agricultural Research Institute, Afyon, Turkey. In addition, the pregnancy rates of experimental animals were investigated using a combined therapy of a progesterone releasing intravaginal device (PRID) and pregnant mare serum gonadotropin (PMSG). The ovaries of each buffalo were examined by rectal palpation once a week and the milk progesterone level was detected twice a week using enzyme immunoassay …

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The Effects Of Indomethacin, Furosemide And Prostaglandin F2 Alpha On Thyroid Hormone Levels In Rams, İzzet Karahan, İbrahi̇m Pi̇ri̇nçci̇, Ahmet Ateşşahi̇n, Feri̇t Gürsu, Gürkan Çikim, Osman Güler Jan 2002

The Effects Of Indomethacin, Furosemide And Prostaglandin F2 Alpha On Thyroid Hormone Levels In Rams, İzzet Karahan, İbrahi̇m Pi̇ri̇nçci̇, Ahmet Ateşşahi̇n, Feri̇t Gürsu, Gürkan Çikim, Osman Güler

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the changes occurring in the levels of serum thyroid hormone in rams given indomethacin, furosemide and PGF2\alpha. In the study, 20 Akkaraman rams, approximately 2 years old were used. Indomethacin (3.0 mg/kg), furosemide (2.0 mg/kg) and PGF2\alpha (0.1 mg/kg) were intramuscularly given to the animals in one dose and repeated for four consecutive days. Blood samples were collected after drug administrations. Free-total thyriiodothyrionine and thyroxine (T3 and T4) levels were determined using a hormone autoanalyser. Serum free T3 and T4 levels in rams given PGF2\alpha were found to have fallen compared to the …

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Seroprevalence Of Bovine Babesiosis Around Kayseri, Abdullah İnci̇, Ayşe Çakmak, K. Zafer Karaer, Şükran Di̇nçer, Fahri̇ Sayin, Anil İça Jan 2002

Seroprevalence Of Bovine Babesiosis Around Kayseri, Abdullah İnci̇, Ayşe Çakmak, K. Zafer Karaer, Şükran Di̇nçer, Fahri̇ Sayin, Anil İça

Turkish Journal of Veterinary & Animal Sciences

This study was carried out on cattle to detect the seroprevalence of babesiosis around Kayseri between July 1997 and January 1999. During this period a total of 191 cattle were examined for the Babesia species. Blood samples were collected from the cattle to obtain sera. During sampling, thick and thin blood smears were prepared from the punctured tail of each animal. Ticks were collected from the animals sampled. The sera were tested for the presence of antibodies to the Babesia species by IFAT. The blood smears were stained with 5% Giemsa's stain solution, and were examined microscopically with oil immersion. …

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Detection Of Infectious Bronchitis Virus In Tracheal Organ Cultures Using The Immunoperoxidase Technique, Ayşi̇n Şen, Gürsel Sönmez, Vi̇ldan Caner, M. Özgür Özyi̇ği̇t Jan 2002

Detection Of Infectious Bronchitis Virus In Tracheal Organ Cultures Using The Immunoperoxidase Technique, Ayşi̇n Şen, Gürsel Sönmez, Vi̇ldan Caner, M. Özgür Özyi̇ği̇t

Turkish Journal of Veterinary & Animal Sciences

The applicability of the immunoperoxidase (IP) technique in unfixed-fresh tracheal organ cultures (TOCs) was investigated for rapid detection of infectious bronchitis virus (IBV). For this purpose, TOCs prepared from one-day-old SPF chicks were inoculated with IBV M41 strain in serial dilutions. IBV antigen detected by IP technique in TOCs 18 h postinoculation. IP staining was observed before siliostasis in all dilutions. The IP technique was also applied to detect IBV antigen in formalin-fixed, paraffin-embedded sections of TOCs inoculated with IBV M41 strain. Viral antigen was also detected in these sections. Histopathological examinations of IBV M41 infected tracheal rings showed loss …

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Colonization, Translocation And Antibody Response In Experimental Campylobacter Infection Of Chickens, Hakan Yardimci, Jale Erdeğer, Mehmet Akan, Murat Yildirim Jan 2002

Colonization, Translocation And Antibody Response In Experimental Campylobacter Infection Of Chickens, Hakan Yardimci, Jale Erdeğer, Mehmet Akan, Murat Yildirim

Turkish Journal of Veterinary & Animal Sciences

In this study we examined factors affecting the colonization of campylobacters in chick intestines, colonization times and elicited immune responses. In chicks, infected orally by minimal infective dose _{100} (MID_{100}), colonization in the intestines was observed to reach 100% during the 3-week period following C. jejuni and C. coli inoculation. When the SDS-PAGE protein profiles of the inoculated and isolated strains were examined similarities of 98.3% among C. jejuni strains and 98.5% among C. coli strains were determined. The results obtained from the ELISA tests performed with blood sera in the group infected with C. jejuni and in the control …

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Studies On Early Diagnosis Of Rhodococcus Equi Infection By Elisa In Foals From Racehorse Breeding Studfarms In The Marmara Region, N. Yakut Özgür, Serkan İki̇z, Arzu Funda Bağcigi̇l, Babür Cari̇oğlu, Ömer Akay, Ati̇lla Ilgaz, Shinji Takai Jan 2002

Studies On Early Diagnosis Of Rhodococcus Equi Infection By Elisa In Foals From Racehorse Breeding Studfarms In The Marmara Region, N. Yakut Özgür, Serkan İki̇z, Arzu Funda Bağcigi̇l, Babür Cari̇oğlu, Ömer Akay, Ati̇lla Ilgaz, Shinji Takai

Turkish Journal of Veterinary & Animal Sciences

Due to the recent increasing mortality rates in 1- to 4-month-old Thoroughbred foals with Rhodococcus equi infection in Turkey, serum samples from 454 foals between 30 and 60 days on studfarms of the Marmara Region, where the Thoroughbred foal population is concentrated, were investigated by ELISA for early diagnosis of the infection. The Tween 20-extracted antigen of the R. equi ATCC 6939 strain was used in the ELISA. Two hundred and nine (46%) of the 454 foals were found to be ELISA-positive. Optical density (OD) values of 0.3 (positive limit) or higher were detected in 43% of foals aged 30-40 …

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Identification Of Chicken Originated Campylobacter Coli And Campylobacter Jejuni By Polymerase Chain Reaction (Pcr), Hasan Basri̇ Ertaş, Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Öngör Jan 2002

Identification Of Chicken Originated Campylobacter Coli And Campylobacter Jejuni By Polymerase Chain Reaction (Pcr), Hasan Basri̇ Ertaş, Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Öngör

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to isolate Campylobacter species from the intestines and livers of chicken and to identify Campylobacter coli and Campylobacter jejuni by both conventional methods and Polymerase Chain Reaction (PCR). Four specific primers derived from the ceuE gene present in the genomes of C. coli and C. jejuni were used for PCR identification. In the examination of 150 intestine and liver samples by culture and PCR, 25 (16.6%) and 32 (21.3%) were identified as C. coli and C. jejuni, respectively. It was concluded that the PCR assay used in this study may successfully be applied for …

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