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Chulalongkorn University

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Full-Text Articles in Veterinary Medicine

Amino Acid Profile Of Rabbit Meat: Dietary Intake And The Effect Of Freezing On The Amino Acid Composition, Alaa Eldin M.A. Morshdy, Wageh Sobhy Darwish, El Sayed S. El Sebay, El-Shaimaa Mesallam Ali Jun 2022

Amino Acid Profile Of Rabbit Meat: Dietary Intake And The Effect Of Freezing On The Amino Acid Composition, Alaa Eldin M.A. Morshdy, Wageh Sobhy Darwish, El Sayed S. El Sebay, El-Shaimaa Mesallam Ali

The Thai Journal of Veterinary Medicine

Rabbit meat is considered as a healthy meat due to its high protein and low fat content. This study aimed at demonstrating the amino acids profile in three rabbit breeds, namely New Zealand White, California and Balady rabbits marketed in Egypt. In addition, the estimated daily intake (EDI) of amino acids due to the consumption of the rabbit meat were calculated. Furthermore, the effect of long frozen storage for different periods on the amino acid content of the New Zealand White rabbit meat was investigated as an experimental trial. The obtained results indicate a slight variation in the amino acid …


Effects Of A Single Dose Hcg Administration On The Induction Of Ovulation, Follicular Dynamics, Uterine Changes And Pregnancy Outcomes In Mares During The Breeding Season, Mesut Çevi̇k, Oğuzhan Ergi̇n, Burcu Esi̇n, Burcu Esi̇n Jun 2022

Effects Of A Single Dose Hcg Administration On The Induction Of Ovulation, Follicular Dynamics, Uterine Changes And Pregnancy Outcomes In Mares During The Breeding Season, Mesut Çevi̇k, Oğuzhan Ergi̇n, Burcu Esi̇n, Burcu Esi̇n

The Thai Journal of Veterinary Medicine

In this study, 19 mares brought to the clinic at different times with the complaint of not getting pregnant were followed up. For this purpose, changes in the uterine and ovarian dynamics of mares and behavioral changes in estrus were followed and evaluated. In particular, it was attempted to increase the chance and rate of pregnancy by providing ovulation with ovulation follow-up followed by a single dose of hCG administration (Chorulon, 3.000 IU). All manipulations and applications in the study were carried out between March and July, that is, during the breeding season of the mares. During the study, a …


Serological Diagnostic For Bovine Paratuberculosis In The Municipality Of Sotaquirá, Colombia, Alejandra Buitrago-López, Alejandra Buitrago-López, Alejandra Buitrago-López, Diana M. Bulla-Castañeda, Adriana M. Díaz-Anaya, Diego J. Garcia-Corredor, Julio Tobón Torreglosa, Diego Ortiz Ortega, Martín O. Pulido-Medellín Jun 2022

Serological Diagnostic For Bovine Paratuberculosis In The Municipality Of Sotaquirá, Colombia, Alejandra Buitrago-López, Alejandra Buitrago-López, Alejandra Buitrago-López, Diana M. Bulla-Castañeda, Adriana M. Díaz-Anaya, Diego J. Garcia-Corredor, Julio Tobón Torreglosa, Diego Ortiz Ortega, Martín O. Pulido-Medellín

The Thai Journal of Veterinary Medicine

Bovine Paratuberculosis (BPT) or Johne’s disease is a pathology caused by Mycobacterium avium subsp. paratuberculosis (MAP). The parasite causes chronic granulomatous enteritis in domestic and wild grazers. Oral-fecal transmission is the most common form of transmission with calves less than six months old being the most susceptible but not presenting clinical symptoms until five years later. It is a disease that spreads during dairy production generating economic loss due to low productive yield and the early discarding of infected animals. The objective of our study was to perform a serological diagnostic of BPT in herds from the municipality of Sotaquirá, …


Complementary Effects Of White Rose Petal Extract On Canine Atopic Dermatitis, Seo-Jin Park, Taesik Yun, Yoonhoi Koo, Yeon Chae, Dohee Lee, Jooyoung Park, Yujin Choi, Ehn-Kyoung Choi, Hakhyun Kim, Yun-Bae Kim, Mhan-Pyo Yang, Byeong-Teck Kang Jun 2022

Complementary Effects Of White Rose Petal Extract On Canine Atopic Dermatitis, Seo-Jin Park, Taesik Yun, Yoonhoi Koo, Yeon Chae, Dohee Lee, Jooyoung Park, Yujin Choi, Ehn-Kyoung Choi, Hakhyun Kim, Yun-Bae Kim, Mhan-Pyo Yang, Byeong-Teck Kang

The Thai Journal of Veterinary Medicine

This study aimed to investigate the therapeutic efficacy of white rose petal extract (WRPE) in canine atopic dermatitis (CAD). Experiment 1 involved clinical assessments using atopic dogs and experiment 2 used beagles with experimentally induced dermatitis. The atopic dogs were divided into four groups: 1% WRPE solution (group 1), 0.5% WRPE solution (group 2), 1% WRPE cream (group 3) and 1% WRPE cream containing lipid granules (group 4). Three normal beagles were used for the experimental induction of dermatitis. Experimental dermatitis was induced by intradermal injections of histamine dihydrochloride, compound 48/80 or substance P. WRPE solution or cream was applied …


Recombinant Scfv Inhibit Newcastle Disease Virus In Vitro Infection, Sanfeng Peng, Lintian Yu, Ying He, Dongyang Chen, Li Wang, Xueliang Zhou, Long Xie, Yangqing Lu Jun 2022

Recombinant Scfv Inhibit Newcastle Disease Virus In Vitro Infection, Sanfeng Peng, Lintian Yu, Ying He, Dongyang Chen, Li Wang, Xueliang Zhou, Long Xie, Yangqing Lu

The Thai Journal of Veterinary Medicine

Newcastle disease (ND) is an avian respiratory disease caused by the Newcastle disease virus (NDV) and is prevalent worldwide but lacks a reliable treatment to surmount it. The single-chain fragment variable (scFv) antibody is the smallest functional unit in an immunoglobulin retaining antigen-binding activities. Previous studies have demonstrated that by acting against the NDV phosphoprotein, scFv is capable of neutralizing the virus in infected cells. In this study, two anti-NDV scFv expressing cassettes driven by CAG promoter and EF1α promoter, respectively, were constructed using the piggyBac transposon plasmid backbone. The recombinant plasmids were transfected into DF-1 and 293T cells and …


Genomic And Phylogenetic Analysis Of The Orf7b Gene From A Chinese Feline Infectious Peritonitis Virus Isolate, Bo Dong, Bo Dong, Xiaodong Zhang, Xiaodong Zhang, Yafei Song, Chengyu Li, Gaoqiang Zhang, Weiming Lin Jun 2022

Genomic And Phylogenetic Analysis Of The Orf7b Gene From A Chinese Feline Infectious Peritonitis Virus Isolate, Bo Dong, Bo Dong, Xiaodong Zhang, Xiaodong Zhang, Yafei Song, Chengyu Li, Gaoqiang Zhang, Weiming Lin

The Thai Journal of Veterinary Medicine

FCoV viruses exhibit great genetic diversity, leading to the presence of FIPV-causing variants. Current molecular evolution analysis and genetic variation studies of FCoV in China are predominately focused on gene encoding the spike protein or other structural proteins, while few studies have evaluated genetic variations in nonstructural FCoV genes, which can play an important role in disease pathogenesis. In this study, the gene encoding the open reading frame (ORF) 7b nonstructural FCoV protein of the Chinese Fujian strain FJLY20201 was amplified from the ascitic fluid of a Chinese domestic cat infected with FIPV and compared with ORF 7b from previously …


Occurrence And Characteristics Of Salmonella Isolated From Various Vegetable Sources: Potential For The Human-Food Interface In Salmonellosis In Vientiane, The Capital Of Laos Pdr, Dethaloun Meunsene, Thanaporn Eiamsam-Ang, Prapas Patchanee, Ratchadaporn Boripun, Phacharaporn Tadee, Sayaphet Rattanavong, Manivanh Vongsouvath, Pakpoom Tadee Jun 2022

Occurrence And Characteristics Of Salmonella Isolated From Various Vegetable Sources: Potential For The Human-Food Interface In Salmonellosis In Vientiane, The Capital Of Laos Pdr, Dethaloun Meunsene, Thanaporn Eiamsam-Ang, Prapas Patchanee, Ratchadaporn Boripun, Phacharaporn Tadee, Sayaphet Rattanavong, Manivanh Vongsouvath, Pakpoom Tadee

The Thai Journal of Veterinary Medicine

Salmonella is one of the most frequently reported causes of bacterial foodborne outbreaks in the world. Despite this, cases associated with raw vegetable consumption have increased, however very few studies have focused on this. The present study is an overview of the role of vegetables as a concern for Salmonella circulating in Vientiane, the capital of the Lao People's Democratic Republic (Lao PDR). A cross-sectional study was surveyed during the first half 2019 on various types of vegetable sold in retailers in the area. The prevalence, serotype, antimicrobial resistance pattern and genetic profile of Salmonella were investigated. In this study, …


Yield, Composition, Fatty Acid Profile And Cla Content Of Milk From Goats Fed With Different Levels Of Opf, Pitunart Noosen, Ulia Renfelia Baysi Jun 2022

Yield, Composition, Fatty Acid Profile And Cla Content Of Milk From Goats Fed With Different Levels Of Opf, Pitunart Noosen, Ulia Renfelia Baysi

The Thai Journal of Veterinary Medicine

The objective of this study was to evaluate the effect of different inclusion levels of OPF in total mixed ration (TMR)-based diet on yield, composition, FA profile and CLA content of milk in dairy goats. Six nulliparous female dairy goats (Saanen x Thai native, 19-21 months old, first lactation) were randomly set according to a 3x3 Latin square design with 2 replications for receiving diets consisted of 0% OPF (OPF0), 20% OPF (OPF20) and 40% OPF (OPF40). The average weight of the goats was 32.48±1.30 kg (mean±s.d.). The findings of this study showed that dry matter intake (DMI) and all …


Inconsistent And Multiple Bacterial Species From Different Sample Types Of Dogs With Urolithiasis And Bacterial Cystitis, Nantapatt Pridsadangbud, Chantamanee Chantarasiri, Nattanit Visitkitjakarn, Phitsinee Tantivimonkajor, Jitrapa Yindee, Nicole Sirisopit Mehl Jun 2022

Inconsistent And Multiple Bacterial Species From Different Sample Types Of Dogs With Urolithiasis And Bacterial Cystitis, Nantapatt Pridsadangbud, Chantamanee Chantarasiri, Nattanit Visitkitjakarn, Phitsinee Tantivimonkajor, Jitrapa Yindee, Nicole Sirisopit Mehl

The Thai Journal of Veterinary Medicine

Urinary tract infection is the most common complication found in dogs with urolithiasis. Bacterial cystitis can play an important role in dogs with urolithiasis; it can either be the cause of cystic formation or be a complication following urolithiasis. Moreover, urolithiasis cases that come with bacterial cystitis can lead to a condition of complicated cystitis which will require more complicated treatment and management. Proper bacterial identification should be performed in order to follow an appropriate treatment. Specific treatments require bacterial identification in order to select the most appropriate antibiotics. There are three main possible sites for bacterial sample collection in …


Detection And Phylogenetic Analysis Of Porcine Epidemic Diarrhea Virus Based On The Orf3 Gene In Pigs In The North Of Vietnam, Tran Duc Hoan, Doan Thi Thao, Nguyen Thi Huong Giang, Nguyen Thi Thu Huyen, Nguyen Thi Thu Huyen Jun 2022

Detection And Phylogenetic Analysis Of Porcine Epidemic Diarrhea Virus Based On The Orf3 Gene In Pigs In The North Of Vietnam, Tran Duc Hoan, Doan Thi Thao, Nguyen Thi Huong Giang, Nguyen Thi Thu Huyen, Nguyen Thi Thu Huyen

The Thai Journal of Veterinary Medicine

To identify further the infectivity, genetic diversity and molecular characteristics of the porcine epidemic diarrhea virus (PEDV). 286 samples of the intestinal tissue of pigs with severe diarrhea were assessed from 5 provinces in the north of Vietnam. The full-length ORF3 gene of 20 representative field strains from 36 farms in 5 provinces was sequenced and analyzed. The prevalence of PEDV was 90.56% and was detected in 259 of 286 samples, with 94.44% (34 of 36) of pig farms harboring the disease. Based on the phylogenetic analysis of the ORF3 genes, our isolates all fell into group G2 (variants) and …


Distribution Of Salmonella Spp. And Serovars Isolated From Captive Reptiles In Four Zoos Of The Zoological Park Organization, Thailand, Between 2017 And 2018, Sompoth Weerakhun, Paweena Kongsanan, Wichit Kongkham Jun 2022

Distribution Of Salmonella Spp. And Serovars Isolated From Captive Reptiles In Four Zoos Of The Zoological Park Organization, Thailand, Between 2017 And 2018, Sompoth Weerakhun, Paweena Kongsanan, Wichit Kongkham

The Thai Journal of Veterinary Medicine

Salmonellosis is the main reason for food borne diseases in many countries globally and it has been found that reptiles are carriers of the disease. Therefore, this study was performed to determine the distribution of Salmonella spp. and their serovars from reptiles in zoos in Thailand between 2017 and 2018. A total of 257 reptile samples were collected from four zoos which are Dusit (128 samples), Chiangmai (32 samples), Songkhla (34 samples) and Nakhonratchasima (63 samples). Salmonella was isolated and identified, serotyping of those isolates was investigated using a panel of Salmonella antisera and serotypes were characterized according to the …


Investigation Of The Microstructure And Mechanical Properties Of The Domestic Cat’S Paw Pads, Ye Mei Jun 2022

Investigation Of The Microstructure And Mechanical Properties Of The Domestic Cat’S Paw Pads, Ye Mei

The Thai Journal of Veterinary Medicine

Domestic cats have a biological function such as cushioning when they are jumping or walking which is mainly due to the biological coupling system formed in the long-term evolution process of nature, including the trunk-leg-paw pad structure. Among them, the paw pad, as the only part of the body that directly contacts with the ground in the process of movement, plays a key role in its biological function. Hence, in order to clarify the micro structure, mechanical properties and functional characteristics of the paw pad and reveal the relationship between it and the domestic cat’s biological function, this paper has …


Optimal Infusion Rate Of Hypertonic Saline To Encephalic Vessel Vasodilatation Using Magnetic Resonance Imaging In Normovolemic Dogs, Yoshiki Murakami, Kenji Tsukano, Haruyuki Hirata, Hiroshi Koie, Kazuyuki Suzuki Jun 2022

Optimal Infusion Rate Of Hypertonic Saline To Encephalic Vessel Vasodilatation Using Magnetic Resonance Imaging In Normovolemic Dogs, Yoshiki Murakami, Kenji Tsukano, Haruyuki Hirata, Hiroshi Koie, Kazuyuki Suzuki

The Thai Journal of Veterinary Medicine

This study aimed to investigate, using magnetic resonance imaging, whether continuous hypertonic saline (HSS) infusion induces a superior vasodilating effect on the encephalic vessels compared to bolus infusion in dogs. The relative cross-sectional area of superior sagittal sinus increased to 1.96±0.09 and 1.69±0.31 times of the pre-value in the continuous and bolus groups, respectively, at t=30 min (p<0.001). However, the relative cross-sectional area of superior sagittal sinus was larger in the continuous group than that in the bolus group (p<0.001). The results suggest that continuous infusion of HSS might be superior to bolus infusion in inducing vasodilatation of the encephalic circulation.


Immunochromatographic Test (Ict) For The Detection Of Antibody Against Alv-J, Guihua Wang, Yanming Wang, Ziqiang Cheng Jun 2022

Immunochromatographic Test (Ict) For The Detection Of Antibody Against Alv-J, Guihua Wang, Yanming Wang, Ziqiang Cheng

The Thai Journal of Veterinary Medicine

ALV-J is an important pathogen in the poultry industry in China. In this study, we developed an immunochromatographic test (ICT) assay for the detection of antibody against of ALV-J to evaluate ALV-J infection. Gp85 protein of ALV-J was employed as a capture antigen. Antigen-antibody reaction was detected by either colloidal-gold conjugated gp85 protein or monoclonal antibody of ALV-J. The high consistency of results in ICT and ELISA indicated that the ICT is reliable. This suggests that the gp85-based ICT can be used as a rapid on-site diagnostic method of ALV-J.


Oxygenated Dressing Quickens Skin Wound Healing Of Bama Miniature Pigs, Yingqiu Wang, Limin Qiao, Hong An, Zhenhao Zhang, Yan Zheng, Chang Chen, Jingrui Zhang, Xiangguo Wang Jun 2022

Oxygenated Dressing Quickens Skin Wound Healing Of Bama Miniature Pigs, Yingqiu Wang, Limin Qiao, Hong An, Zhenhao Zhang, Yan Zheng, Chang Chen, Jingrui Zhang, Xiangguo Wang

The Thai Journal of Veterinary Medicine

Oxygen is a vital factor in the process of wound healing and is involved in collagen synthesis, synthesis and the release of growth factors, angiogenesis and epidermal cell contraction. Hence, local oxygen control of wounds plays an important role in skin wound healing. Herein, we developed a novel oxygenated dressing for the treatment of skin wound healing in Bama miniature pigs. The oxygenated dressing was mainly composed of potassium iodide, glucose, glucose oxidase, polyvinyl alcohol, polypropylene, polyvinylpyrrolidone and water. The oxygenated dressing significantly quickens the healing of full-thickness skin wounds in Bama miniature pigs and promotes the proliferation of epidermal …


Supplementation Of Extracellular Adenosine 5'-Triphosphate Improves Sperm Post-Thawed Quality In Thai Native Chickens, Phakjira Sanguansook, Junpen Suwimonteerabutr, Kaywalee Chatdarong Jun 2022

Supplementation Of Extracellular Adenosine 5'-Triphosphate Improves Sperm Post-Thawed Quality In Thai Native Chickens, Phakjira Sanguansook, Junpen Suwimonteerabutr, Kaywalee Chatdarong

The Thai Journal of Veterinary Medicine

Thai native chicken meat is a high-quality protein source of unique flavor. Genetic preservation and breeding programs are beneficial to maintain purebred populations. Aiming at improving post-thawed sperm quality, this study is the first to apply extracellular adenosine 5'-triphosphate (ATPe) in freezing extender to preserve Thai native chicken sperm. Eight semen samples from eight Thai native chickens (n = 8) were included. Initial semen evaluation and sperm size measurement were performed. Beltsville poultry semen extender supplemented with 0 and 30 mM ATPe was served as a control and treatment group, respectively. After thawing, motility and motion characteristics were evaluated by …


Diagnostic Imaging And Hematological And Cytological Analyses Play A Vital Role In Clinical Investigation And Selection Of Therapeutic Approaches In A Polo Pony With Septic Olecranon Bursitis, Rungrueang Yodsheewan, Ravisara Limsripan, Metha Chanda Jun 2022

Diagnostic Imaging And Hematological And Cytological Analyses Play A Vital Role In Clinical Investigation And Selection Of Therapeutic Approaches In A Polo Pony With Septic Olecranon Bursitis, Rungrueang Yodsheewan, Ravisara Limsripan, Metha Chanda

The Thai Journal of Veterinary Medicine

A 19-year-old gelding polo pony was presented with a prominent protuberance at the tip of the right elbow and five days later, developed a marked swelling on the entire right foreleg with a high fever. Ultrasonography, performed on the caudal elbow region, demonstrated heterogeneous echogenic materials encapsulated in the fibrous capsule. The initial hematological analysis revealed anemia and a normal white blood count (WBC). Hyperbilirubinemia was detected biochemically. Pale yellow/turbid fluid was aspirated from the protuberance area, which showed septic exudate characteristics and contained Streptococcus spp. The pony was diagnosed with septic olecranon bursitis associated with streptococcal infection. The fluid-filled …


Stifle Joint Reconstruction Technique Using Fascia Lata In A Cat With Cranial Cruciate Ligament Rupture, Hern-Yeu Lee, Wei-Yau Shia, Tien-Huan Hsu, Wei-Ming Lee Jun 2022

Stifle Joint Reconstruction Technique Using Fascia Lata In A Cat With Cranial Cruciate Ligament Rupture, Hern-Yeu Lee, Wei-Yau Shia, Tien-Huan Hsu, Wei-Ming Lee

The Thai Journal of Veterinary Medicine

A two-year-old spayed female mongrel cat presenting with right hind limb lameness with intermittent weight bearing while walking was taken to the veterinary medicine teaching hospital of National Chung Hsing University. Both drawer test and tibial compression test were positive, stifle radiography showed synovial fluid had become more radiopaque. The tentative diagnosis for the cat was rupture of the right cranial cruciate ligament (CrCL), medical management using corticosteroids was prescribed with anticoagulants and surgical treatment recommended. Intracapsular reconstruction was performed and the postoperative recovery of this cat presented normal physical status with improved right hind limb weight-bearing activity. This report …


Mycotic Pyogranulomatous Enteritis In A German Shepherd: Imaging Diagnosis And Treatment, Phasamon Saisawart, Somchin Sutthigran, Apichart Srifawattana, Onjira Mitroupathum, Tanhatai Krajarngjang, Anudep Rungsipipat, Nan Choisunirachon Jun 2022

Mycotic Pyogranulomatous Enteritis In A German Shepherd: Imaging Diagnosis And Treatment, Phasamon Saisawart, Somchin Sutthigran, Apichart Srifawattana, Onjira Mitroupathum, Tanhatai Krajarngjang, Anudep Rungsipipat, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

A five-year old, intact male, German Shepherd dog was presented to the hospital due to a history of depression, anorexia, vomiting and diarrhea. Physical examination revealed moderate emaciation and showed mild abdominal pain when Palpation. Abdominal radiographs and ultrasounds showed the distension of the small bowel loop due to partial obstruction of the colon by mural concentric thickening. Computed tomography (CT) scan showed an homogeneous, soft tissue mass with asymmetric thickening of the cecum traversing the colonic wall. Pre-operative blood profiles indicated normocytic normochromic mild anemia, hypoalbuminemia and mild hyperglobulinemia. Intestinal resection and anastomosis at the distal ileum to the …


Feline Inductive Odontogenic Tumor In A Young Cat, Chakkarin Satthathum, Sunee Kunakornsawat, Chaiyan Kasorndorkbua Jun 2022

Feline Inductive Odontogenic Tumor In A Young Cat, Chakkarin Satthathum, Sunee Kunakornsawat, Chaiyan Kasorndorkbua

The Thai Journal of Veterinary Medicine

A 6-month-old, 4.45 kg, intact, male domestic shorthair cat was presented with a gingival mass located on the left rostral maxilla and second left upper premolar tooth. Intraoral x-ray of the left rostral maxilla revealed left maxilla bone lysis with a unilocular cystic lesion. The cytology result revealed an epithelial cell tumor .A left rostral maxillectomy was performed and the tumor was submitted for histological assessment .Histopathological diagnosis of the tissue revealed a feline inductive odontogenic tumor .There was no evidence of tumor recurrence 24 months after surgery.


African Swine Fever Vaccine Development: Current Status And Challenges Ahead, Sezim Monoldorova, Galina Koltsova, Ilya Titov, Inkyu Yoo, Andrey Gogin, Jonguk Jeong, Hyeon-Seop Byeon, Seungri Yun, Yunhee Chang, Shin-Seok Kang, Denis Kolbasov, Bo-Young Jeon Mar 2022

African Swine Fever Vaccine Development: Current Status And Challenges Ahead, Sezim Monoldorova, Galina Koltsova, Ilya Titov, Inkyu Yoo, Andrey Gogin, Jonguk Jeong, Hyeon-Seop Byeon, Seungri Yun, Yunhee Chang, Shin-Seok Kang, Denis Kolbasov, Bo-Young Jeon

The Thai Journal of Veterinary Medicine

African swine fever (ASF) is a contagious, hemorrhagic and highly lethal viral disease in swine. Following the initial report of ASF in Kenya in 1921 it spread to East Asia through Western Europe and Russia (including Eastern Europe). Many efforts have been dedicated to controlling ASF but the struggle to eradicate this disease continues. Thus far, the efforts to develop an effective vaccine to control ASF have been unsuccessful. A previous inactivated ASF vaccine, developed by traditional methods, failed to protect pigs from ASF virus (ASFV) infection. Neutralizing antibodies were not effective in inducing protective immunity and it appears that …


Evaluation Of Changes In Some Enzymatic And Non-Enzymatic Antioxidants In Cats With Feline Panleukopenia, Afshin Behzadian, Ameneh Khoshvaghti Mar 2022

Evaluation Of Changes In Some Enzymatic And Non-Enzymatic Antioxidants In Cats With Feline Panleukopenia, Afshin Behzadian, Ameneh Khoshvaghti

The Thai Journal of Veterinary Medicine

Feline panleukopenia is a serious contagious viral disease of cats that has been identified as the causative agent of death in 25% of kittens sent for pathological examination. Antioxidant systems are one of the very important defense systems of body. The aim of the current study was to evaluate changes in serum levels of Vitamin D, glutathione peroxidase and superoxide dismutase in cats infected with panleukopenia virus. Ten healthy male cats and ten male cats with clinical and laboratory symptoms of panleukopenia with a mean age of 6±2 months were selected. The Glutathione peroxidase and superoxide dismutase were measured by …


Indications For Canine Fecal Microbiota Transplantation, Kerong Li, Xiaoxiao Zhou, Zhijun Zhong, Haifeng Liu, Min Li, Guangneng Peng, Ziyao Zhou Mar 2022

Indications For Canine Fecal Microbiota Transplantation, Kerong Li, Xiaoxiao Zhou, Zhijun Zhong, Haifeng Liu, Min Li, Guangneng Peng, Ziyao Zhou

The Thai Journal of Veterinary Medicine

Fecal microbiota transplantation (FMT) is a novel method to treat intestinal and extraintestinal diseases by transplanting the microbiota in the feces of healthy animals into the gastrointestinal tracts of patients and reconstructing intestinal flora in the patients' intestine. In this article, we reviewed the recent studies of canine FMT focusing on its indications. FMT was proved effective to treat canine Clostridioides difficile infection (CDI), inflammatory bowel disease (IBD), canine parvovirus (CPV) diarrhea and acute diarrhea; upregulate the intestinal microbiota in dogs with acute hemorrhagic diarrhea syndrome (AHDS); and may have benefit for preventing canine post-weaning diarrhea. However, the specific FMT …


Transcutaneous Light Penetration Of Simultaneous Superpulsed And Multiple Wavelength Photobiomodulation Therapy In Living Dog Tissue, Somphong Hoisang, Suvaluk Seesupa, Supranee Jitpean, Naruepon Kampa Mar 2022

Transcutaneous Light Penetration Of Simultaneous Superpulsed And Multiple Wavelength Photobiomodulation Therapy In Living Dog Tissue, Somphong Hoisang, Suvaluk Seesupa, Supranee Jitpean, Naruepon Kampa

The Thai Journal of Veterinary Medicine

Photobiomodulation therapy (PBMT) is used as an adjunctive therapy for wound healing, pain relief and rehabilitation. The penetration of light to the target tissue is the crucial factor. The aim of this study was to investigate the potential penetration of simultaneous superpulsed and multiple wavelength (SPMW) PBMT on living tissue in dogs. Twenty client-owned dogs were anesthetized and underwent abdominal surgery. The PMBT device was set at 50 Hz and delivered light for 60 sec at different distances; at 0 cm (contact) and 1 cm tissue-device distance (non-contact). The power meter was placed and measured the mean output power (MOP) …


Expression Of Prostaglandin F2 Alpha Receptors (Ptgfr) And C-Reactive Protein (Crp) In The Canine Uterus And Cervix With Opened- And Closed-Cervix Pyometra, Narong Tiptanavattana, Pokchon Khirilak, Theerawat Tharasanit, Natapol Duangtum, Kijsopon Jankaew, Prawit Sae-Jia, Nongnapat Kaewchana Mar 2022

Expression Of Prostaglandin F2 Alpha Receptors (Ptgfr) And C-Reactive Protein (Crp) In The Canine Uterus And Cervix With Opened- And Closed-Cervix Pyometra, Narong Tiptanavattana, Pokchon Khirilak, Theerawat Tharasanit, Natapol Duangtum, Kijsopon Jankaew, Prawit Sae-Jia, Nongnapat Kaewchana

The Thai Journal of Veterinary Medicine

Pyometra is a common reproductive disorder in female dogs. This study investigated the protein expression of prostaglandin F2 alpha receptors (PTGFR) and C-reactive protein (CRP) in responses to inflammatory events of uterine and cervical tissues in dogs. Uterus and cervix samples were collected from six dogs which had closed-cervix pyometra (n = 3) and opened- cervix pyometra (n = 3). Healthy dogs in diestrus (n = 3) and anestrus (n=3) were used as controls. Tissues were histologically prepared and stained with hematoxylin and eosin. Immunohistochemistry (IHC) and western blot analysis for the PTGFR and CRP were used to verify the …


Seasonal And Antimicrobial Resistance Distributions Of Salmonella Isolated From Pork, Beef And Chicken Meat In Vientiane Capital, Lao Pdr, Sitthasone Xaymountry, Kanit Chukanhom, Seri Klangair, Jatesada Jiwakanon, Sunpetch Angkititrakul Mar 2022

Seasonal And Antimicrobial Resistance Distributions Of Salmonella Isolated From Pork, Beef And Chicken Meat In Vientiane Capital, Lao Pdr, Sitthasone Xaymountry, Kanit Chukanhom, Seri Klangair, Jatesada Jiwakanon, Sunpetch Angkititrakul

The Thai Journal of Veterinary Medicine

The objectives in this study was to investigate the prevalence, seasonal variation and antimicrobial resistance of Salmonella isolated from pork, beef and chicken meat at retail markets in Vientiane capital, Lao PDR. During May 2018 to April 2019, 445 swab samples were randomly collected from pork (n = 258), beef (n = 81) and poultry meat (n = 106) in three seasons at three retail big markets in Vientiane capital, Lao PDR, Thongkhankham, Lao-Aussi, and Nongchan markets. All samples were isolated and identified for Salmonella spp. using the ISO 6579:2002/AMD 1:2007 procedure. The isolates were tested for antimicrobial susceptibility using …


Investigation Of Bacterial Pathogens In Milk From Mastitic Dairy Cattle By Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry, Gokben Ozbey, Baris Otlu, Yusuf Yakupogullari, Betul Celik, Elif Seren Tanriverdi, Neslihan Kelestemur, Tarik Safak, Ali Risvanli, Anil Persad, Emma Sproston Mar 2022

Investigation Of Bacterial Pathogens In Milk From Mastitic Dairy Cattle By Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry, Gokben Ozbey, Baris Otlu, Yusuf Yakupogullari, Betul Celik, Elif Seren Tanriverdi, Neslihan Kelestemur, Tarik Safak, Ali Risvanli, Anil Persad, Emma Sproston

The Thai Journal of Veterinary Medicine

The scope of the present study was to assess the use of Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry (MS) as a quick technique for the identification of bacterial species in mastitis. In this study, milk samples from each udder quarter from a total of 250 dairy cattle were aseptically collected and tested. The samples were grouped into California Mastitis Test (CMT) positive, CMT negative and clinical mastitis. The samples were streaked on blood agar and the bacterial isolates were analysed using MALDI-TOF MS. Using MALDI-TOF MS, certain species such as Staphylococcus chromogenes (44/188, 23.4%), Aerococcus viridans (40/188, 21.3%) …


Inflammatory Mediator Gene Expression Of The Kidneys Induced By Daboia Siamensis Venom And Its Components, Suchitra Khunsap, Sunutcha Suntrarachun, Orawan Khow, Jureeporn Noiphrom Mar 2022

Inflammatory Mediator Gene Expression Of The Kidneys Induced By Daboia Siamensis Venom And Its Components, Suchitra Khunsap, Sunutcha Suntrarachun, Orawan Khow, Jureeporn Noiphrom

The Thai Journal of Veterinary Medicine

Phospholipase A2 (PLA2) and metalloprotease (MP) from Daboia siamensis (Ds) venom are believed to contribute to renal tissue toxicity. Imbalance of the cytokine system induced by snake envenomation causes tissue injury and cell damage which leads to acute toxic kidney injury. A better understanding of the pathological mechanism involved in renal injury after venom administration is needed. In the present study, we aimed to investigate inflammatory mediator genes (IL-2, IL-10, TNFα, IFN, and COX-2) in mouse kidney tissue after venom injection. Quantitative real time PCR was performed to detect the cytokine gene profiles. Beta actin was used as a house-keeping …


Therapeutic Potential Of Bulgarian Propolis Against Antibiotic-Resistant Salmonella Species, Manar M. Farouk, Amal El-Molla, Fayez A. Salib, Yousef A. Soliman, Mohamed Shaalan Mar 2022

Therapeutic Potential Of Bulgarian Propolis Against Antibiotic-Resistant Salmonella Species, Manar M. Farouk, Amal El-Molla, Fayez A. Salib, Yousef A. Soliman, Mohamed Shaalan

The Thai Journal of Veterinary Medicine

The emergence of multidrug-resistant (MDR) Salmonella spp. has increased the need for investigation of antimicrobial alternatives. Ethanolic extract of propolis (EEP) has been identified to have wide-ranging medical applications related to its antimicrobial activity. The current study aimed to identify the major chemical components of EEP with antibacterial effects, evaluate the antibacterial efficacy of EEP against MDR Salmonella spp. isolates, and further examine the therapeutic efficacy of EEP against MDR S. Enteritidis infected mouse model. Bulgarian propolis EEP was prepared by using ethanol based extraction method. The chemical composition of EEP was characterized using the gas chromatography-mass spectroscopy (GC-MS). The …


The Effect Of Recombinant Chimeric Swine Pkr-Apaf-1 Proteins On Viral-Load Reduction Of Prrs And Ped Alpha-Coronavirus Infected Cell Lines, Phong Vu Anh Tuan Vo, Thanaporn Lertsakchai, Sukeesom Jiramahasuwan, Achara Tawatsin, Athipoo Nuntaprasert Mar 2022

The Effect Of Recombinant Chimeric Swine Pkr-Apaf-1 Proteins On Viral-Load Reduction Of Prrs And Ped Alpha-Coronavirus Infected Cell Lines, Phong Vu Anh Tuan Vo, Thanaporn Lertsakchai, Sukeesom Jiramahasuwan, Achara Tawatsin, Athipoo Nuntaprasert

The Thai Journal of Veterinary Medicine

The PRRS virus and PED alpha-coronavirus are the two main pig pathogens in the world. The current strategies to control these diseases are inadequate. The recombinant chimeric swine PKR-Apaf-1 protein (rcPAP) consists of the Human Immunodeficiency Virus Trans-activator transcription (HIV-TAT) domain, the dsRNA-binding domain of porcine Protein kinase R (PKR) gene and the caspase recruitment domain (CARD) domain of porcine Apoptotic Protease-Activating Factor-1 (Apaf-1) gene. In general, the rcPAP can detect viral dsRNA in virally infected cells and induces apoptosis and kills the virus-infected cells. In this study, the reducing viral-load efficacy of rcPAP (produced from bacteria) against PRRS virrus …