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Articles 631 - 660 of 5680
Full-Text Articles in Veterinary Medicine
Development And Validation Of A Scale For Measuring Pet Owners' Loyalty To Their Pet*, Seda Çavuş Alan, Abdullah Özen, Nurhan Hali̇sdemi̇r
Development And Validation Of A Scale For Measuring Pet Owners' Loyalty To Their Pet*, Seda Çavuş Alan, Abdullah Özen, Nurhan Hali̇sdemi̇r
Turkish Journal of Veterinary & Animal Sciences
Loyalty is a person's willingness to devote oneself to a cause and consists of concepts such as fidelity, not giving up, continuity, and stability. For this reason, this relationship stems from loyalty to be able to talk about a strong attachment between humans and animals. This study aimed to develop a measurement tool for the evaluation of the loyalty levels of people toward their pets. The qualitative method for determining the judgments in the scale will be called the pet loyalty scale; the quantitative methods (explanatory factor analysis and confirmatory factor analysis) were used to determine the validity and reliability …
Genotyping Of The Κ - Casein Gene By Pcr - Rflp (Acui Digestion) And Its Relationship To Milk Yield Traits In Anatolian Buffaloes, Murrah Buffaloes, And Their Crosses, Orhan Karadağ, Vasfi̇ye Kader Esen, Mehmet Aki̇f Yüksel, Mustafa Küçükkebabçi, Semi̇h Sevi̇m, İlkay Çeti̇n, Bi̇nnur Kaptan, Mehmet İhsan Soysal
Genotyping Of The Κ - Casein Gene By Pcr - Rflp (Acui Digestion) And Its Relationship To Milk Yield Traits In Anatolian Buffaloes, Murrah Buffaloes, And Their Crosses, Orhan Karadağ, Vasfi̇ye Kader Esen, Mehmet Aki̇f Yüksel, Mustafa Küçükkebabçi, Semi̇h Sevi̇m, İlkay Çeti̇n, Bi̇nnur Kaptan, Mehmet İhsan Soysal
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the polymorphism of the κ - casein gene (kappa casein, CSN3) in buffaloes and determine its effects on milk yield traits. Data were obtained from 2016 to 2019 from all animals in the ongoing Anatolian Buffalo Breeding Project. Genomic DNA was collected from a total of 209 buffaloes of different ages and sexes, namely 31 Murrah, 46 Anatolian buffaloes, and 132 of their crosses (Murrah × Anatolian). Genotyping of CSN3 gene by polymerase chain reaction-restriction fragment length polymorphism (PCR - RFLP) technique with restriction enzyme (AcuI) revealed three …
A Comparative Profile Of Certain Biochemical And Hematological Parameters In Angora And Akkeçi Goats During The Transition Period, Rabi̇a Aktaş, Erkan Pehli̇van
A Comparative Profile Of Certain Biochemical And Hematological Parameters In Angora And Akkeçi Goats During The Transition Period, Rabi̇a Aktaş, Erkan Pehli̇van
Turkish Journal of Veterinary & Animal Sciences
This study investigated the comparative changes in hematological and certain biochemical parameters such as glucose, cholesterol, triglyceride calcium, phosphorus and total protein during the transition period in Angora and Akkeçi goats aged 2-4 years. For this purpose, blood samples were regularly taken from the vena jugularis of each goat at 3, 2, and 1 weeks before kidding (-3, -2 and -1), on the day of parturition (0) and 1, 2, and 3 weeks after kidding (+1, +2 and +3). According to the statistical analysis results, the differences between the averages of the groups in terms of MCHC, MCH, Lym, THR, …
Serum Trace Element Levels In Dogs With Canine Visceral Leishmaniasis, Aycan Nuri̇ye Gazyağci, Bengü Bi̇lgi̇ç, Berna Baysal Bakay, Duygu Tarhan, Alev Meltem Ercan, Songül Erdoğan, Hasan Erdoğan, Mehmet Erman Or, Kerem Ural
Serum Trace Element Levels In Dogs With Canine Visceral Leishmaniasis, Aycan Nuri̇ye Gazyağci, Bengü Bi̇lgi̇ç, Berna Baysal Bakay, Duygu Tarhan, Alev Meltem Ercan, Songül Erdoğan, Hasan Erdoğan, Mehmet Erman Or, Kerem Ural
Turkish Journal of Veterinary & Animal Sciences
Canine visceral leishmaniasis has been denoted as neglected despite being a very well-known disease. Trace element alteration has been recognized in humans with visceral and cutaneous leishmaniasis, together with canine visceral leishmaniasis. The trace elements occupy a vital position in the immunological system, and host immune responses mitigate defense against leishmaniasis. We aimed to select trace elements in a total of 45 dogs of several breeds; those at the age of 11 months to 6 and from both sexes (26 male and 19 female) were enrolled in the study. The dogs included in the study were divided into leishmaniasis-infected and …
Host-Genetic-Based Outcome Of Co-Infection By Pcv2b And Prrsv In Pigs, Christian W. Eaton, Hiep Vu, Arabella L. Hodges, Seth P. Harris, Stephen D. Kachman, Daniel C. Ciobanu
Host-Genetic-Based Outcome Of Co-Infection By Pcv2b And Prrsv In Pigs, Christian W. Eaton, Hiep Vu, Arabella L. Hodges, Seth P. Harris, Stephen D. Kachman, Daniel C. Ciobanu
School of Veterinary and Biomedical Sciences: Faculty Publications
Replication of porcine circovirus type 2 (PCV2), an important worldwide swine pathogen, has been demonstrated to be influenced by host genotype. Specifically, a missense DNA polymorphism (SYNGR2 p.Arg63Cys) within the SYNGR2 gene was demonstrated to contribute to variation in PCV2b viral load and subsequent immune response following infection. PCV2 is known to induce immunosuppression leading to an increase in susceptibility to subsequent infections with other viral pathogens such as porcine reproductive and respiratory syndrome virus (PRRSV). In order to assess the role of SYNGR2 p.Arg63Cys in co-infections, pigs homozygous for the favorable SYNGR2 p.63Cys (N = 30) …
Use Of Amino Acid Isotope Analysis To Investigate Capital Versus Income Breeding Strategies In Migratory Avian Species, Nico Lübcker, John P. Whiteman, Oliver N. Shipley, Keith A. Hobson, Seth D. Newsome
Use Of Amino Acid Isotope Analysis To Investigate Capital Versus Income Breeding Strategies In Migratory Avian Species, Nico Lübcker, John P. Whiteman, Oliver N. Shipley, Keith A. Hobson, Seth D. Newsome
Biological Sciences Faculty Publications
1. Income and capital breeding represent opposing ends of a continuum of reproductive strategies. Quantifying nutrient allocation to reproduction is challenging, but recent advances in compound-specific stable isotope analysis hold promise for tracing the source of individual compounds allocated to reproduction.
2. Here, we describe a novel approach of using measured carbon (δ13C) and nitrogen (δ15N) isotope values of individual amino acids (AAs) in pectoral muscle of egg-laying females and egg yolk as a useful tool to quantify the reliance on income versus capital breeding in migrating species. We used white-fronted (Anser albifrons frontalis), lesser snow (A. …
Financial Strain Of Covid-19 And Its Impact On Willingness-To-Pay For Equine Care, Jada M. Thompson, Michelle Kibler, Jennie L.Z. Ivey
Financial Strain Of Covid-19 And Its Impact On Willingness-To-Pay For Equine Care, Jada M. Thompson, Michelle Kibler, Jennie L.Z. Ivey
Agricultural Economics and Agribusiness Faculty Publications and Presentations
The novel COVID-19 virus caused a global pandemic disrupting lives, industries, and economies. The result was an impact on prices due to challenges with production and supply chain distribution. This study investigates the financial strain COVID-19 had on equine owners and leasers, what the market for equine care would bear if costs for care increased, and what factors contribute to their willingness to pay (WTP) for increasing cost of equine care. An online survey was distributed for 4 weeks to adult U.S. residents. Respondents reported their involvement in the equine industry, financial response to COVID-19, and responses to …
Secondary Traumatic Stress Is An Occupational Hazard For Pet Hospice Support Staff: Psychoeducational Support Groups Provide The Cure, Kari Hayden
DSW Capstone Projects
There are three separate yet interrelated products presented in this final capstone product. The completed product is focused on pet hospice veterinary support staff and the need for an evidence-based social work intervention to combat secondary traumatic stress. This capstone uses the andragogical approach to adult learning theory as the foundation to construct a psychoeducational peer support group specific to this population. Implications for clinical social work in veterinary medicine are presented within this project.
The first scholarly product presented is the Systematic Literature Review (SLR). The SLR utilizes a scoping review process in an attempt to locate prior research …
Influence Of Lactobacillus Plantarum Administration On Some Serum/Tissue (Uterus) Antioxidant And Cytokine Levels In Female Rats Exposed To Cadmium (Cd), İhsan Kisadere, Bariş Güner, Hakan Tavşanli, Mehmet Faruk Aydin, Mustafa Usta
Influence Of Lactobacillus Plantarum Administration On Some Serum/Tissue (Uterus) Antioxidant And Cytokine Levels In Female Rats Exposed To Cadmium (Cd), İhsan Kisadere, Bariş Güner, Hakan Tavşanli, Mehmet Faruk Aydin, Mustafa Usta
Turkish Journal of Veterinary & Animal Sciences
The study aimed to investigate the effects of oral Lactobacillus plantarum (Lp) administration on some serum/tissue (uterus) antioxidant and cytokine levels as well as histopathologic changes in female rats exposed chronically to Cd. For this purpose, rats were separated into four groups including control (C, n = 8), cadmium (Cd, n = 8), Lp (n = 8), and Cd + Lp (n = 8). Cd (dissolved in water at a dose of 2.04 mg/mL) was orally administered to Cd and Cd + Lp groups for 28 days. Besides, active-live Lp, which was grown at a level of approximately 10$^{8}$-10$^{9}$ cfu/mL …
Effect Of Acute And Repeated Noise Exposure On The Behaviour And Lipid Peroxidation In Brain Tissue Of Male And Female Mice, Gabriella Korsós, Kinga Fodor, Annamária Kiss, Anna Blázovics, Sándor György Fekete
Effect Of Acute And Repeated Noise Exposure On The Behaviour And Lipid Peroxidation In Brain Tissue Of Male And Female Mice, Gabriella Korsós, Kinga Fodor, Annamária Kiss, Anna Blázovics, Sándor György Fekete
Turkish Journal of Veterinary & Animal Sciences
Despite the increasingly obvious sex differences, male animals are still predominantly used to study stress and related disorders. The aim of this study was to evaluate the effects of repeated noise stress in female and male mice. In the experiment, 12 male and 12 and female SPF/VAF 6-week-old Crl:CD1(lcr) mice were used. After 5 days of acclimatization all animals were placed in the open-field for three consecutive days where each mouse spent 10 min daily without any noise treatment in order to get used to the circumstances of the test. On day 9 to record baseline behaviour the animals were …
Defining And Exploring Animal Sentience, Andrew N. Rowan, Joyce M. D'Silva Mrs, Ian J.H. Duncan, Nicholas Palmer
Defining And Exploring Animal Sentience, Andrew N. Rowan, Joyce M. D'Silva Mrs, Ian J.H. Duncan, Nicholas Palmer
Animal Sentience
One of the commentaries on the target article notes that "animal sentience" is difficult to define operationally. This response to the commentaries develops a working, usable definition of animal sentience and examines the relationships between animal emotions and sentience.
Influence Of Temperature-Humidity Index, Calf Sex, And Parity On Gestation Length In Holstein Cows, Lazoumi Ouarfli, Abdelmadjid Chehma
Influence Of Temperature-Humidity Index, Calf Sex, And Parity On Gestation Length In Holstein Cows, Lazoumi Ouarfli, Abdelmadjid Chehma
Turkish Journal of Veterinary & Animal Sciences
The present study aimed to analyse the gestation length (GL) variability in locally born purebred Holstein dams (n = 1047), in a private farm, under Saharan climate. The overall GL mean was 278.73 ± 6.67 days. A general linear model revealed that the GL was significantly affected by calf sex (p = 0.035) with a minor increase of 0.19% in males, dam parity (p = 0.016) and calving season (p < 0.0001) including the shortest lengths recorded in heifers and during heat stress period. Moreover, a negative regression (-1.96%) in recent years was recorded in GL by the influence (p = 0.01) of years of birth, breeding, and calving of cows. Our findings show that cows that were inseminated and gave birth during the cold season (temperature-humidity index, THI < 74) had 11.54 days longer (p = 0.048) gestation period compared to animals that were inseminated and gave birth during the hot season (THI > 84). Similarly, the association (p = 0.003) between dam parity and calf sex revealed that the GL of multiparous cows that gave birth to male calves was higher (3.07 days) …
The Efficiency Of Toltrazuril, Thymol, And Toltrazuril + Thymol Combination On Eimeriosis In Lamb, Mehmet Özüi̇çli̇, Ahmet Onur Gi̇ri̇şgi̇n, İhsan Kisadere, Ali̇ İhsan Di̇ker, Ali̇ Erdi̇ Candar, Levent Aydin
The Efficiency Of Toltrazuril, Thymol, And Toltrazuril + Thymol Combination On Eimeriosis In Lamb, Mehmet Özüi̇çli̇, Ahmet Onur Gi̇ri̇şgi̇n, İhsan Kisadere, Ali̇ İhsan Di̇ker, Ali̇ Erdi̇ Candar, Levent Aydin
Turkish Journal of Veterinary & Animal Sciences
This study aimed to evaluate and compare the efficacy of the treatment with a single dose of toltrazuril 5% (Baycox), thymol (Versatile, Nine Life, United Arab Emirates), and toltrazuril + thymol combination to control Eimeria spp. infection in lambs in intensive feeding. The study was designed with five groups and these groups aligned as; group 1 (negative control group; NEG), group 2 (positive control group; POZ), group 3 (thymol; THY), group 4 (toltrazuril; TOL) and group 5 [(thymol + toltrazuril; (THY + TOL)]. Each group consisted of 26 Merino lambs which are 60-75 days old each. Treatment groups were administered …
Using Different Levels Of Coated Calcium Sodium Butyrate In The Diet Of Quail On Growth Performance, Duodenal Histomorphology, And Some Biochemical Parameters, Mükremi̇n Ölmez, Tarkan Şahi̇n, Şükran Yedi̇el Aras, Özlem Karadağoğlu, Ebru Karadağ Sari, Hati̇ce Gi̇zem Büyükbaki̇, Ebrar Fi̇li̇zoğlu, İdi̇l Şerbetçi̇
Using Different Levels Of Coated Calcium Sodium Butyrate In The Diet Of Quail On Growth Performance, Duodenal Histomorphology, And Some Biochemical Parameters, Mükremi̇n Ölmez, Tarkan Şahi̇n, Şükran Yedi̇el Aras, Özlem Karadağoğlu, Ebru Karadağ Sari, Hati̇ce Gi̇zem Büyükbaki̇, Ebrar Fi̇li̇zoğlu, İdi̇l Şerbetçi̇
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine growth performance, duodenal histomorphology, and some biochemical parameters in quails' diets containing coated calcium sodium butyrate (CSB). One hundred sixty 1-day-old Japanese quails were arranged into four trial groups consisting of 5 replicates with eight chicks per replicate. One group was formed as a control group without CSB, and the other experimental groups were formed by adding CSB at the level of 0.06% (CSB - 1), 0.08% (CSB - 2), and 0.1% (CSB - 3). All diets were prepared isonitrogenically and isocalorically, with 24% CP and 3000 kcal/kg ME content. The trial …
Investigation Of Relationships Between Serum Lactate, Acute Phase Proteins, Pro/ Antiinflammatory Cytokine Levels, And Metritis Formation In Holstein Dairy Heifers, Talha Avcilar, Davut Koca, Yavuz Nak, Mustafa Eren Şahi̇n, Musa Özgür Özyi̇ği̇t, Deni̇z Nak
Investigation Of Relationships Between Serum Lactate, Acute Phase Proteins, Pro/ Antiinflammatory Cytokine Levels, And Metritis Formation In Holstein Dairy Heifers, Talha Avcilar, Davut Koca, Yavuz Nak, Mustafa Eren Şahi̇n, Musa Özgür Özyi̇ği̇t, Deni̇z Nak
Turkish Journal of Veterinary & Animal Sciences
The main purpose of this investigation was to identify and suggest suitable early screening inflammatory markers for the early diagnosis of uterine infections such as acute septic metritis (ASM) in the Holstein heifers. In addition, to achieve the above -mentioned aim, the objectives of the present study were tried to determine the relationship between blood serum lactate, acute phase proteins (APPs), pro/anti inflammatory cytokine levels, and metritis formation in Holstein heifers. For this purpose, 250 Holstein heifers which were 11-13 months old, were chosen as the study material. After heifers were conceived, blood serum samples were collected at the 8th …
Effect Of Different Light Intensities And Colors On Growth Performance And Reproductive Characteristics In Japanese Quails, Fawwad Ahmad, Muhammad Sharif, Muhammad Ashraf, Muhammad Riaz, Muhammad Shoaib, Talha Siddique, Aqian Ahmed, Ahmad Hamad Sheir, Muhammad Ahsan Yaseen, Muhammad Mahboob Ali Hamid
Effect Of Different Light Intensities And Colors On Growth Performance And Reproductive Characteristics In Japanese Quails, Fawwad Ahmad, Muhammad Sharif, Muhammad Ashraf, Muhammad Riaz, Muhammad Shoaib, Talha Siddique, Aqian Ahmed, Ahmad Hamad Sheir, Muhammad Ahsan Yaseen, Muhammad Mahboob Ali Hamid
Turkish Journal of Veterinary & Animal Sciences
Light is one of the most important environmental factors in quail farming, as it has a significant impact on production efficiency and reproductive characteristics. The purpose of this study was to determine the effect of different light intensities and colors on production performance in Japanese quails. For the first experiment, 200 Japanese quails were divided into five treatment groups, each with four replicates (10 birds in each). The birds in the control treatment were exposed to a light intensity of 20 lux, while the other four treatment groups were exposed to light intensities of 10 lux, 15 lux, 25 lux, …
The Effect Of Simvastatin Treatment On Tnf-Alpha-Induced Inflammation In Human Vascular Endothelial Cells (Huvecs), Büşra Görgün, Betül İşi̇ner Kaya, Kübra Kaya, Leyla Di̇dem Kozaci
The Effect Of Simvastatin Treatment On Tnf-Alpha-Induced Inflammation In Human Vascular Endothelial Cells (Huvecs), Büşra Görgün, Betül İşi̇ner Kaya, Kübra Kaya, Leyla Di̇dem Kozaci
Turkish Journal of Veterinary & Animal Sciences
This study investigates the effects of simvastatin (SMV) on human umbilical vein endothelial cells (HUVECs) inflamed by tumor necrosis factor-alpha (TNF-a). Cells were treated with simvastatin (5 μM, 10 μM, and 50 μM) with/without TNF (20 ng/mL). The effects of simvastatin on the proliferation of cells were examined, and its cytotoxic effect was determined by lactate dehydrogenase (LDH) assays. Metalloproteinase expression (MMP-2 and MMP-9) levels of SMV at different concentrations were determined by qPCR, and protein levels were determined by Western blot. The increasing concentrations of SMV down-regulated the expression levels of MMPs. After stimulation with TNF-a, the effect of …
Investigation Of Bmp15 And Gdf9 Gene Polymorphisms And Their Effects On Litter Size In Anatolian Sheep Breed Akkaraman, Koray Kirikçi
Investigation Of Bmp15 And Gdf9 Gene Polymorphisms And Their Effects On Litter Size In Anatolian Sheep Breed Akkaraman, Koray Kirikçi
Turkish Journal of Veterinary & Animal Sciences
This study aimed to examine the polymorphism of the BMP15 (FecX$^{G}$ and FecX$^{I}$) and GDF9 (G1 and G4) genes and the association between genotypes and litter size in the Akkaraman breed. Blood samples were taken from 100 ewes in total. Genomic DNA was obtained from whole blood. The PCR-RFLP technique was employed to determine the polymorphisms of the genes under investigation. The G1 was the only polymorphic among the four loci analyzed. The frequencies for the GA and GG genotypes were 0.26 and 0.74, respectively, while the A and G allele frequencies were 0.13 and 0.87, respectively. The observed and …
Could There Be A Relationship Between Feeding Characteristics And The Shape Of Condylus Occipitalis And Foramen Magnum In Mammals?, Yasi̇n Demi̇raslan, İsmai̇l Demi̇rci̇oğlu, İftar Gürbüz, Funda Aksünger Karaavci, Abdullah Ortadeveci, Özcan Özgel
Could There Be A Relationship Between Feeding Characteristics And The Shape Of Condylus Occipitalis And Foramen Magnum In Mammals?, Yasi̇n Demi̇raslan, İsmai̇l Demi̇rci̇oğlu, İftar Gürbüz, Funda Aksünger Karaavci, Abdullah Ortadeveci, Özcan Özgel
Turkish Journal of Veterinary & Animal Sciences
The aim of the study was to investigate the shape of the foramen magnum and condylus occipitalis of mammalian species belonging to different feeding groups. For this, two-dimensional geometric morphometric method analyses were applied. The data from the skulls of the 12 species were used. In principal component analysis, the first three principal components accounted for 70.353%of the total shape variation. According to the first principal component, the dorsal edge of the condylus occipitalis and foramen magnum contributed the most to shape variation. Most of the individuals were correctly separated according to the feeding groups. The most accurate grouping features …
Mites [Of Dogs And Cats], Susan E. Little, Roberto Cortinas
Mites [Of Dogs And Cats], Susan E. Little, Roberto Cortinas
School of Veterinary and Biomedical Sciences: Faculty Publications
Veterinary parasitology: 20,000-word article details recognition and treatments for mite infestations of dogs and cats. Mite species reviewed include:
Cheyletiella spp. (“Walking Dandruff”)
Demodex spp. (Hair Follicle Mites, Red Mange, Demodicosis, Demodectic Mange)
Sarcoptes scabiei (Itch Mite, Scabies, Sarcoptic Mange)
Notoedres cati (Feline Head and Neck Mange)
Otodectes cynotis (Ear Mites)
Pneumonyssoides caninum (Nasal Mite)
Lynxacarus radovskyi (Fur Mite, Hair Clasping Mite)
Trombiculid Mites (Chiggers, Red Bugs, Berry Bugs, Harvest Mites, Scrub Itch or Grass Itch Mites)
Discussions include Etiology and epidemiology, Pathogenesis, Clinical features, Diagnosis, Treatment and prognosis, Immunity, Prevention, and Public health aspects.
Complete with 209 references.
More People, More Cats, More Parasites: Human Population Density And Temperature Variation Predict Prevalence Of Toxoplasma Gondii Oocyst Shedding In Free-Ranging Domestic And Wild Felids, Sophie Zhu, Elizabeth Vanwormer, Karen Shapiro
More People, More Cats, More Parasites: Human Population Density And Temperature Variation Predict Prevalence Of Toxoplasma Gondii Oocyst Shedding In Free-Ranging Domestic And Wild Felids, Sophie Zhu, Elizabeth Vanwormer, Karen Shapiro
School of Veterinary and Biomedical Sciences: Faculty Publications
Toxoplasma gondii is a ubiquitous zoonotic parasite that can infect warm-blooded vertebrates, including humans. Felids, the definitive hosts, drive T. gondii infections by shedding the environmentally resistant stage of the parasite (oocysts) in their feces. Few studies characterize the role of climate and anthropogenic factors in oocyst shedding among free-ranging felids, which are responsible for the majority of environmental contamination. We determined how climate and anthropogenic factors influence oocyst shedding in free-ranging domestic cats and wild felids using generalized linear mixed models. T. gondii oocyst shedding data from 47 studies were systematically reviewed and compiled for domestic cats and six …
Chemical Characterization And Biological Evaluation Of Epilobium Parviflorum Extracts In An In Vitro Model Of Human Malignant Melanoma, Sotiris Kyriakou, Venetia Tragkola, Ioannis Paraskevaidis, Mihalis Plioukas, Dimitrios T. Trafalis, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidi
Chemical Characterization And Biological Evaluation Of Epilobium Parviflorum Extracts In An In Vitro Model Of Human Malignant Melanoma, Sotiris Kyriakou, Venetia Tragkola, Ioannis Paraskevaidis, Mihalis Plioukas, Dimitrios T. Trafalis, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidi
School of Veterinary and Biomedical Sciences: Faculty Publications
Malignant melanoma is an aggressive type of skin cancer characterised by high metastatic capacity and mortality rate. On the other hand, Epilobium parviflorum is known for its medicinal properties, including its anticancer potency. In this context, we aimed to (i) isolate various extracts of E. parviflorum, (ii) characterize their phytochemical content, and (iii) determine their cytotoxic potential in an in vitro model of human malignant melanoma. To these ends, we utilized various spectrophotometric and chromatographic (UPLC-MS/MS) approaches to document the higher content of the methanolic extract in polyphenols, soluble sugars, proteins, condensed tannins, and chlorophylls -a and -b as …
Detection Of Mannheimia Haemolytica-Specific Igg, Igm And Iga In Sera And Their Relationship To Respiratory Disease In Cattle, Korakrit Poonsuk, Carita Kordik, Matthew M. Hille, Ting-Yu Cheng, William B. Crosby, Amelia R. Woolums, Michael L. Clawson, Carol G. Chitko-Mckown, Bruce Brodersen, John Dustin Loy
Detection Of Mannheimia Haemolytica-Specific Igg, Igm And Iga In Sera And Their Relationship To Respiratory Disease In Cattle, Korakrit Poonsuk, Carita Kordik, Matthew M. Hille, Ting-Yu Cheng, William B. Crosby, Amelia R. Woolums, Michael L. Clawson, Carol G. Chitko-Mckown, Bruce Brodersen, John Dustin Loy
School of Veterinary and Biomedical Sciences: Faculty Publications
Mannheimia haemolytica is one of the major causes of bovine respiratory disease in cattle. The organism is the primary bacterium isolated from calves and young cattle affected with enzootic pneumonia. Novel indirect ELISAs were developed and evaluated to enable quantification of antibody responses to whole cell antigens using M. haemolytica A1 strain P1148. In this study, the ELISAs were initially developed using sera from both M. haemolytica-culture-free and clinically infected cattle, then the final prototypes were tested in the validation phase using a larger set of known-status M. haemolytica sera (n = 145) collected from feedlot cattle. The test …
First Gene-Edited Calf With Reduced Susceptibility To A Major Viral Pathogen, Aspen M. Workman, Michael P. Heaton, Brian L. Vander Ley, Dennis A. Webster, Luke Sherry, Jonathan R. Bostrom, Sabrina Larson, Theodore S. Kalbfleisch, Gregory P. Harhay, Erin E. Jobman, Daniel F. Carlson, Tad S. Sonstegard
First Gene-Edited Calf With Reduced Susceptibility To A Major Viral Pathogen, Aspen M. Workman, Michael P. Heaton, Brian L. Vander Ley, Dennis A. Webster, Luke Sherry, Jonathan R. Bostrom, Sabrina Larson, Theodore S. Kalbfleisch, Gregory P. Harhay, Erin E. Jobman, Daniel F. Carlson, Tad S. Sonstegard
School of Veterinary and Biomedical Sciences: Faculty Publications
Bovine viral diarrhea virus (BVDV) is one of the most important viruses affecting the health and well-being of bovine species throughout the world. Here, we used CRISPR-mediated homology-directed repair and somatic cell nuclear transfer to produce a live calf with a six amino acid substitution in the BVDV binding domain of bovine CD46. The result was a gene-edited calf with dramatically reduced susceptibility to infection as measured by reduced clinical signs and the lack of viral infection in white blood cells. The edited calf has no off-target edits and appears normal and healthy at 20 months of age without obvious …
Does Swab Type Matter? Comparing Methods For Mannheimia Haemolytica Recovery And Upper Respiratory Microbiome Characterization In Feedlot Cattle, William B. Crosby, Lee Pinnell, John T. Richeson, Cory Wolfe, Jake Castle, John Dustin Loy, Sheryl P. Gow, Keun Seok Seo, Sarah F. Capik, Amelia R. Woolums, Paul S. Morley
Does Swab Type Matter? Comparing Methods For Mannheimia Haemolytica Recovery And Upper Respiratory Microbiome Characterization In Feedlot Cattle, William B. Crosby, Lee Pinnell, John T. Richeson, Cory Wolfe, Jake Castle, John Dustin Loy, Sheryl P. Gow, Keun Seok Seo, Sarah F. Capik, Amelia R. Woolums, Paul S. Morley
School of Veterinary and Biomedical Sciences: Faculty Publications
Background: Bovine respiratory disease (BRD) is caused by interactions among host, environment, and pathogens. One standard method for antemortem pathogen identification in cattle with BRD is deep-guarded nasopharyngeal swabbing, which is challenging, costly, and waste generating. The objective was to compare the ability to recover Mannheimia haemolytica and compare microbial community structure using 29.5 inch (74.9 cm) deep-guarded nasopharyngeal swabs, 16 inch (40.6 cm) unguarded proctology swabs, or 6 inch (15.2 cm) unguarded nasal swabs when characterized using culture, real time-qPCR, and 16S rRNA gene sequencing. Samples for aerobic culture, qPCR, and 16S rRNA gene sequencing were collected from the …
Aerobic Exercise And Scaffolds With Hierarchical Porosity Synergistically Promote Functional Recovery Post Volumetric Muscle Loss, Christina Zhu, Karina Skylar, Yori Endo, Mohamadmahdi Samandari,, Mehran Karvar, Azadeh Mostafavi, Jacob Quint, Chiara Rioldi, Wojciech Swieszkowski, Joshua Mauney, Shailesh Agarwal, Ali Tamayol, Indranil Sinha
Aerobic Exercise And Scaffolds With Hierarchical Porosity Synergistically Promote Functional Recovery Post Volumetric Muscle Loss, Christina Zhu, Karina Skylar, Yori Endo, Mohamadmahdi Samandari,, Mehran Karvar, Azadeh Mostafavi, Jacob Quint, Chiara Rioldi, Wojciech Swieszkowski, Joshua Mauney, Shailesh Agarwal, Ali Tamayol, Indranil Sinha
School of Veterinary and Biomedical Sciences: Faculty Publications
Volumetric muscle loss (VML) is a composite loss of skeletal muscle tissue (greater than 20%) that heals with minimal muscle regeneration, substantial fibrosis, and subsequent functional deficits. Standard treatment, involving free functional muscle transfer and physical therapy, cannot restore full muscle function following VML. Tissue engineered scaffolds, 3D structural templates that mimic native extracellular matrix, are promising to enhance functional muscle formation and recovery. Bioprinted 3D scaffolds are engineered using bioinks, created from scaffolding material, cells, and growth factors, to replicate skeletal muscle architecture with precise control over their spatial deposition. METHODS: The present study evaluates a 3D-printed foam-like scaffold …
Infection Of Feral Phenotype Swine With Japanese Encephalitis Virus, So Lee Park, Yan-Jang S. Huang, Amy C. Lyons, Victoria B. Ayers, Susan M. Hettenbach, D. Scott Mcvey, Leela E. Noronha, Kenneth R. Burton, Stephen Higgs, Dana L. Vanlandingham
Infection Of Feral Phenotype Swine With Japanese Encephalitis Virus, So Lee Park, Yan-Jang S. Huang, Amy C. Lyons, Victoria B. Ayers, Susan M. Hettenbach, D. Scott Mcvey, Leela E. Noronha, Kenneth R. Burton, Stephen Higgs, Dana L. Vanlandingham
School of Veterinary and Biomedical Sciences: Faculty Publications
Background: Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic flavivirus and the leading cause of pediatric encephalitis in the Asian Pacific region. The transmission cycle primarily involves Culex spp. mosquitoes and Ardeid birds, with domestic pigs (Sus scrofa domestica) being the source of infectious viruses for the spillover of JEV from the natural endemic transmission cycle into the human population. Although many studies have concluded that domestic pigs play an important role in the transmission cycle of JEV, and infection of humans, the role of feral pigs in the transmission of JEV remains unclear. Since domestic and …
Detection Of Sars-Cov-2 Neutralizing Antibodies In Retropharyngeal Lymph Node Exudates Of White-Tailed Deer (Odocoileus Virginianus) From Nebraska, Usa, Korakrit Poonsuk, Duan S. Loy, Rachael Birn, Bryan Buss, Matthew Donahue, Todd Nordeen, Kylie Sinclair, Luke Meduna, Bruce Brodersen, John Dustin Loy
Detection Of Sars-Cov-2 Neutralizing Antibodies In Retropharyngeal Lymph Node Exudates Of White-Tailed Deer (Odocoileus Virginianus) From Nebraska, Usa, Korakrit Poonsuk, Duan S. Loy, Rachael Birn, Bryan Buss, Matthew Donahue, Todd Nordeen, Kylie Sinclair, Luke Meduna, Bruce Brodersen, John Dustin Loy
School of Veterinary and Biomedical Sciences: Faculty Publications
Disease surveillance testing for emerging zoonotic pathogens in wildlife is a key component in understanding the epidemiology of these agents and potential risk to human populations. Recent emergence of SARS-CoV-2 in humans, and subsequent detection of this virus in wildlife, highlights the need for developing new One Health surveillance strategies. We used lymph node exudate, a sample type that is routinely collected in hunter-harvested white-tailed deer (WTD, Odocoileus virginianus) for surveillance of chronic wasting disease, to assess anti-SARS-CoV-2 neutralizing antibodies. A total of 132 pairs of retropharyngeal lymph nodes collected from Nebraska WTD harvested in Nebraska, US, in 2019 …
Understanding Human Gut Bacterial Interactions And Their Role In Colonization Resistance Against Clostridioides Difficile Using Mini-Bioreactor Array And Caenorhabditis Elegans As Model Systems, Achuthan Ambat
Electronic Theses and Dissertations
No abstract provided.
Rumen-Protected Methionine Supplementation Improves Lactation, Oxidative Status, And Immune Response During A Subclinical Mastitis Challenge In Lactating Dairy Cows., Anita Paz Lopez
Electronic Theses and Dissertations
Mastitis is a significant problem in the dairy industry, posing numerous challenges for dairy farmers worldwide. Mastitis refers to the inflammation of the mammary gland, primarily caused by bacterial infections. This condition affects the health and well-being of dairy cows and has severe economic implications for farmers and overall dairy production. The prevalence of mastitis substantially threatens milk quality, productivity, and profitability, making it a top concern in the dairy industry. Preventing mastitis in cows is crucial for maintaining the herd's health and ensuring highquality milk production. Nutritional strategies have recently gained greater attention due to a direct impact on …