Pharmacy and Pharmaceutical Sciences Commons^™

Development Of Granule Product Containing Curcuminoid Extract And Hyaluronic Acid Derivative For Oral Administration, Natkhanang Boonpetcharat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Curcumin is widely recognized for its various pharmacological properties, including antioxidant, anti-inflammatory, and anti-tumor activities. Nevertheless, the development of curcumin as a therapeutic agent is impeded by its limited oral bioavailability, which stems from its chemical instability, poor aqueous solubility, and rapid degradation. This study aimed to develop granule formulations incorporating hyaluronic acid graft poly(N-isopropylacrylamide) or HA-g-pNIPAM to enhance dissolution and protect curcumin from degradation. Three formulations were developed: F10 (HA-g-pNIPAM physically mixed with curcumin), F10 Encap (curcumin encapsulated within HA-g-pNIPAM), and F11 (curcumin granules without HA-g-pNIPAM). The stability results showed that F10 Encap effectively maintained curcumin content throughout the …

The Effect Of Stilbene Derivatives On Modulation Of P-Gp Efflux Transporter, Shahnila Mukhtar

Chulalongkorn University Theses and Dissertations (Chula ETD)

Drug efflux by transporters of the ATP-binding cassette (ABC) family such as P-glycoprotein is the earliest and most well-studied multidrug resistant (MDR) mechanism. Overexpression of ATP-binding cassette transporters can decrease the effectiveness of chemotherapy by pumping anti-cancer drugs out of cancer cells. This study was to predict the best-fit of certain stilbenoids isolated from Thai orchids as a specific P-gp moderator, using the molecular docking approach to compare and specify the binding interaction between each stilbenoids and the P-gp binding domains. Furthermore, the in vitro substrate uptake assays of eight stilbenoids with phenanthrene core structure were conducted for assessing their …

Factors Contributing To Self-Medication And Quality Of Life Of Myanmar Immigrants In The Covid-19 Pandemic Situation, Su Myat Thin

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Due to numerous difficulties in accessing necessary healthcare and standard medication, immigrants might be more likely to rely on self-medication to solve health problems they suffered. Although it might provide immediate relief, it could also bring some risks such as adverse drug reactions, antimicrobial resistance progression, drug side effects, and toxicity. This study aimed to identify the healthcare service accessibility and self-medication of immigrants in Thailand for COVID-19. Additionally, this study also aimed to evaluate the effect of immigrants’ COVID-19 perception on their self-medication uses and quality of life. This study had two phases in which the first phase …

The Effect Of Artogomezianone On Apoptosis Induction In Non-Small-Cell Lung Cancer, Worawich Somboonvoravong

Chulalongkorn University Theses and Dissertations (Chula ETD)

NSCLC severity is driven by dysregulation of the EGFR/PI3K/Akt pathway, promoting tumor growth and drug resistance. Hyperactive EGFR/PI3K/Akt signaling results in aggressive tumors and poor outcomes, further worsened by resistance to EGFR inhibitors. Targeting this pathway could inhibit tumor growth and resistance. artogomezianone, a compound derived from Artocarpus gomezianus, has shown considerable anti-cancer efficacy, mainly by inducing apoptosis and decreasing the proliferation of several cancers. This study investigated artogomezianone’s effects on the EGFR/PI3K/Akt pathway. Cytotoxic and apoptotic effects were assessed using MTT assay and Hoechst 33342/PI double staining, with confirmation by Western blot analysis. Protein levels and binding affinity were …

Role Of Edaravone Nanosuspension In Preterm Labor And Birth Outcomes, Sruthi Sarvepalli

Theses and Dissertations

Annually, preterm birth (PTB) affects fifteen million infants, posing a significant risk of infant mortality and lifelong disability. Among various triggers, it is well-established that infection-associated oxidative stress and inflammation can induce preterm labor. There is no FDA approved medication for the prevention of PTB except tocolytics which provide marginal benefit at a later stage. Scarcity of therapeutic intervention and unmet medical need propelled us in investigating a novel drug molecule. Our current research is focused on investigating the potential of Edaravone (EDA), an FDA-approved antioxidant and anti-inflammatory medication, in delaying preterm labor and improving neonatal outcomes. Our goal is …

In Silico Identifcation Of New Potential Inhibitors Of Quorum Sensing By Gram‑Positive Bacteria Through Specialized Molecular Docking, Amgad Albohy, Najla A. Obaid, Mariam Mojally, Mohammed Abou Rehab, Najd Ahmad Alkhudhir

Pharmacy

Quorum sensing is the process by which bacterial cells can communicate by producing substances to regulate viable processes such as gene expression, virulence, and bioflm formation. Gram-positive bacteria such as Staphylococcus aureus and Enterococcus faecalis have specifc enzymes (autoinducers) that control the quorum sensing system. Sortase A is a surface protein that regulates virulence and cell‒cell communication in Gram-positive bacteria. To interfere with this system and reduce virulence and cell‒cell communication, quorum sensing inhibitors are used, which are nonantibiotic substances. In this study, we aimed to use Food and Drug Administration-approved drugs (analgesics and antipsychotics) and investigate their activity using …

Development Of New Radiotracers For Positron Emission Tomography Targeting C-X-C Chemokine Receptor Type 4 In Cancers, Piyapan Suwattananuruk

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to develop novel positron emission tomography (PET) tracers for C-X-C chemokine receptor type 4 (CXCR4) imaging. [68Ga]Ga-TD-01 and [18F]PICO-F were designed as PET tracers based on the molecular scaffolds of two potent CXCR4 antagonists, TIQ15 and PICO. TIQ15 was used to prepare a bifunctional (BFC) to facilitate 68Ga-labeling while structural modification of PICO was made to obtain a precursor, PICO-Br for 18F-labeling. The BFC, TD-01 was obtained in a moderately high yield (61%) via the conjugation of TIQ15 with a chelator derivative and successfully radiolabeled with 68Ga to yield [68Ga]Ga-TD-01, a PET tracer. For 18F-based tracer, the …

Scaffold Hopping For The Lead Optimization Of The Orally Active Antileishmanial Pyrrolo-Quinazoline Alkaloid Natural Product Peganine, Sunitha Jada

Electronic Theses and Dissertations

Leishmaniasis is a protozoan parasitic disease caused by the species Leishmania that affects millions of people around the world. It is a serious public health concern in the developing world with about 1 million cases and 70,000 fatalities occurring each year. It has several forms with visceral, and cutaneous leishmaniasis being the most common forms of the disease. Visceral leishmaniasis, caused by the Leishmania donovani occurs mainly in Brazil, Eastern Africa, and India, and is fatal if left untreated. The current treatments for leishmaniasis such as pentavalent antimonials, amphotericin B, miltefosine, paromomycin, pentamidine, and some azoles suffer from serious drawbacks. …

Research News, Publications, 2024, Volume 2, Soumyajit Majumdar

Publications (2015-)

Publications that appeared during the period of April 1 through June 30, 2024.

Research News, Publications, 2024, Volume 3, Soumyajit Majumdar

Publications (2015-)

Publications that appeared during the period of July 1 through September 30, 2024.

Research News, Publications, 2024, Volume 4, Joshua Sharp

Publications (2015-)

Publications that appeared during the period of October 1 - December 31, 2024.

Research News, Grants, 2024, Volume 3, Soumyajit Majumdar

Grants (2015-)

Grants and contracts awarded during July - September 2024 - Revised 10/22/24

Research News, Grants, 2024, Volume 4, Joshua Sharp

Grants (2015-)

Grants and contracts awarded during October - December 2024

A Real-World Disproportionality Analysis Of Everolimus: Data Mining Of The Public Version Of Fda Adverse Event Reporting System, Bin Zhao, Yumei Fu, Shichao Cui, Xiangning Chen, Shu Liu, Lan Luo

Faculty, Staff and Student Publications

Background: Everolimus is an inhibitor of the mammalian target of rapamycin and is used to treat various tumors. The presented study aimed to evaluate the Everolimus-associated adverse events (AEs) through data mining of the US Food and Drug Administration Adverse Event Reporting System (FAERS).

Methods: The AE records were selected by searching the FDA Adverse Event Reporting System database from the first quarter of 2009 to the first quarter of 2022. Potential adverse event signals were mined using the disproportionality analysis, including reporting odds ratio the proportional reporting ratio the Bayesian confidence propagation neural network and the empirical Bayes geometric …

Targeting The Renin-Angiotensin System (Ras) For Neuropsychiatric Disorders, Aline Silva De Miranda, Danielle S Macedo, Natalia P Rocha, Antonio L Teixeira

Faculty, Staff and Student Publications

BACKGROUND: Neuropsychiatric disorders, such as mood disorders, schizophrenia, and Alzheimer's disease (AD) and related dementias, are associated to significant morbidity and mortality worldwide. The pathophysiological mechanisms of neuropsychiatric disorders remain to be fully elucidated, which has hampered the development of effective therapies. The Renin Angiotensin System (RAS) is classically viewed as a key regulator of cardiovascular and renal homeostasis. The discovery that RAS components are expressed in the brain pointed out a potential role for this system in central nervous system (CNS) pathologies. The understanding of RAS involvement in the pathogenesis of neuropsychiatric disorders may contribute to identifying novel therapeutic …

Treatment Patterns And Clinical And Economic Burden In Patients With Merkel Cell Carcinoma: Statistical And Machine Learning Approaches, Yves Mbous

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although considered a rare form of nonmelanoma skin cancer, Merkel Cell Carcinoma incidence rate has increased to 0.74 per 100,000 and projections expect this number to steadily rise over the next decades and to reach an average of 3,285 cases per year in 2025. MCC incidence increases significantly with age. Among those aged 40-44, it is 0.44, it rises to 1.0/100,000 in the 60-64 age group and soars to 9.8/100,000 among those ≥85 years and above. Due to the small number of cases, MCC is often managed using institutional practices or provider preferences. Formal clinical trials that enroll early stage …

Management Of Factor Xa Inhibitor-Related Traumatic Non-Intracranial Bleeding Events With Andexanet Alfa Or Four-Factor Prothrombin Complex Concentrate In A Us Multicenter Observational Study, Paul P. Dobesh, Craig I. Coleman, Mark Danese, Eva Lesén, Raymond C. Chang, Onivefu Odelade, Gregory J. Fermann

Journal Articles: Pharmacy Practice

OBJECTIVES: This study describes clinical characteristics and management strategies for patients with factor Xa (FXa) inhibitor-related traumatic non-intracranial bleeds who were treated with andexanet alfa or four-factor prothrombin complex concentrate (4F-PCC).

METHODS: An observational cohort study (ClinicalTrials.gov Identifier: NCT05548777) was conducted using electronic health records from 354 US hospitals. Included patients were hospitalized with rivaroxaban- or apixaban-related bleeding, had received andexanet alfa or 4F-PCC treatment during their hospitalization, and were discharged between May 2018 and September 2022. This analysis was performed in the subgroup of patients with traumatic non-intracranial critical compartment/non-compressible bleeds or other traumatic bleeds.

RESULTS: The study population …

Andexanet Alfa Versus Pcc Products For Factor Xa Inhibitor Bleeding: A Systematic Review With Meta-Analysis, C. Michael White, Kimberly Snow Caroti, Youssef Bessada, Adrian V. Hernandez, William L. Baker, Paul P. Dobesh, Heleen Van Haalen, Kirsty Rhodes, Craig I. Coleman

Journal Articles: Pharmacy Practice

Previous meta-analyses assessed andexanet alfa (AA) or prothrombin complex concentrate (PCC) products for the treatment of Factor Xa inhibitor (FXaI)-associated major bleeding. However, they did not include recent studies or assess the impact of the risk of bias. We conducted a systematic review with meta-analysis on the effectiveness of AA versus PCC products for FXaI-associated major bleeding, inclusive of the studies' risk of bias. PubMed and Embase were searched for comparative studies assessing major bleeding in patients using FXaI who received AA or PCC. We used the Methodological Index for NOn-Randomized Studies (MINORS) checklist and one question from the Joanna …

The Impact Of Candida Albicans Glycogen On Fitness, Virulence And Host Response, Jian Miao

Theses and Dissertations (ETD)

Candida albicans is an opportunistic human fungal pathogen that presents in healthy persons colonizing the oropharyngeal, esophageal, and gastrointestinal mucosa. This polymorphic fungal pathogen remains a leading cause of both invasive and superficial mycoses. C. albicans can cause oropharyngeal candidiasis (OPC) and disseminated candidiasis when normal host immunity is disturbed, while also causing vulvovaginal candidiasis (VVC) in healthy women. Adaptation to different host niches requires rapid and coordinated changes in Candida metabolism and physiology to cope with host immune surveillance and to counteract host-imposed constraints such as glucose limitation. Although largely unexplored in C. albicans, the glycogen metabolism pathway is …

Relationship Of Serum Copeptin Level With Traditional Risk Factors For Acute Coronary Syndrome In Early Diagnosis Of This Syndrome In Iraqi Patients, Ali Abdul Rasool Hussein, Abdulkareem H. Issa, Abbas Naji Muslem Al Shareeifi

Al-Esraa University College Journal for Medical Sciences

Background: Acute coronary syndrome (ACS) is still one of the main causes of morbidity and mortality worldwide and an early diagnosis of myocardial infarction (MI) aims to decrease mortality in MI patients. The ACS is manifested as one of three subtypes. These subtypes include MI with the electrocardiogram (ECG) is showing ST-segment elevation (STEMI), the other is MI with the ECG is showing no ST-segment elevation (NSTEMI) and the third type is unstable angina (UA). Objectives: To explore the Relationship of serum copeptin level with traditional risk factors for acute coronary syndrome in early diagnosis of this syndrome in Iraqi …

Immunological And Molecular Investigation Of Non-Helicobacter Pylori Gastritis Within Type 2 Diabetes Mellitus, Zahraa Maad Abdul-Sahib, Najah Ali Mohammad, Abdul Razzaq Nema

Al-Esraa University College Journal for Medical Sciences

Gastritis is one of the most common diseases in the digestive system, especially in patients with low immunity, including diabetic mellitus. Diabetic Mellitus considered one of an endocrine gland disease characterized by elevated blood sugar because of a defect in the regulation of sugar in the body. The purpose of this study was to assess the association between Non-Helicobacter pylori gastritis with type 2 diabetic mellitus (N-Hp G.T2DM), by interleukin IL-10 and IL-18. This study included 37(41.1%) N-Hp G.T2DM, 23 (25.6%) gastritis (G) only and 30 (33.3%) healthy participants. Biomarkers for T2DM and G (FBS, …

Investigating The Association Of Demographic Factors On Methotrexate Delay-Clearance And Toxicity In Pediatric Oncology Patients: A Retrospective Chart Review, Belal Alabdul Razzak

Honors Undergraduate Theses

High-dose methotrexate (HD MTX) is critical for treating pediatric malignancies such as acute lymphoblastic leukemia and neuro-carcinoma. However, its significant toxicity due to drug accumulation poses substantial risks. This retrospective study assesses the impact of demographic factors on MTX toxicity and clearance in pediatric oncology patients. Patient records from Saint Mary Hospital were analyzed, focusing on two MTX administration protocols: a 24-hour infusion followed by alkaline hydration and a 4-hour infusion followed by alkaline hydration. We hypothesize that factors such as age, body surface area (BSA), and body mass index (BMI) are associated with MTX clearance and toxicity. The study …

Strategies And Emerging Mechanisms In Managing Pseudomonas Aeruginosa Biofilm-Associated Antibiotic Resistance In Renal Catheters, Ahmed Fadhil Kadhim

Al-Esraa University College Journal for Medical Sciences

Strategies and mechanisms in controlling Pseudomonas aeruginosa resistance to biofilm-associated with renal catheters are a serious concern. Renal catheters are widely used in hospitals for numerous urological disorders. However, they are the main cause of bacterial infections, especially P. aeruginosa, when used for a long time. The mechanism of biofilm-associated antibiotic resistance of P. aeruginosa in renal catheters is complex and has not been efficiently managed. This review debates certain strategies and mechanisms associated with P. aeruginosa biofilm antibiotic resistance in cases with renal catheters, including the prevention of biofilm formation, eradication of biofilm, and reducing biofilm-associated antibiotic resistance and …

Distribution Of Epidemiological Characteristics Among Drug Addiction Patients Admitted To Alqana Center For Social Rehabilitation, Ali Abdul Alkhudhur Murad, Atta Ah. Mousa Al-Sarray, Zeena Jamal Alkhazraji

Al-Esraa University College Journal for Medical Sciences

Background: Drug addiction is a worldwide issue; 5.6% of the world's population aged 15 to 64 took drugs at least once in 2016. For the majority of medicines, younger individuals use them more than older people do. Drug abuse seems to be on the increase in several ASEAN (Association of Southeast Asian Nations) countries, particularly among young men aged 15 to 30. Illicit drug abuse account for around 14% of the overall health burden among young males. Younger individuals are also more prone to die from drug abuse issues. Aim: To assess the severity of problems related to drug …

Immunological Markers And Clinical Features With Systemic Lupus Erythematosus In Iraqi Women Patients, Ali Yahya. Zeki, Ali Hussein. Al-Hafid, Haider Hafudh Humaish

Al-Esraa University College Journal for Medical Sciences

Background: Systemic Lupus Erythematosus is a complicated condition caused by the immune system attacking disease with unclear causes, affecting mainly women. It manifests with various symptoms and is diagnosed based on clinical and serological signs. Aims: To investigate the prevalence and the type of immunological and hematologic manifestations in SLE from pathologist's perspective. Methods: The study was done on 60 SLE patients and 60 controls from the Consulting Rheumatology Clinic in Baghdad Teaching Hospital in Baghdad City during the first half of the year 2024, from 25^th January 2024 until 25^th April 2024. Results: Marital state …

Cathepsin D And Its Relation With Lipid Profile In Coronary Atherosclerosis Patients, Israa Saad Salim, Walaa Ismael Jassim, Ahmed Saadi Hassan

Al-Esraa University College Journal for Medical Sciences

Background: Coronary atherosclerosis is a long lasting and continuously evolving disease with multiple clinical manifestations ranging from asymptomatic to stable angina, acute coronary syndrome (ACS), heart failure (HF) and sudden cardiac death (SCD). The onset and advancement of coronary atherosclerosis are influenced by both genetic and environmental factors. Objectives: The study aimed to find out if cathepsin D a good indicator to diagnose atherosclerosis and cardiovascular diseases and its relation with lipid profile. Materials and Methods: The study conducted with the participation of 150 subjects (100 patients with coronary atherosclerosis and 50 healthy control). Age for all subject was in …

Secretor Status And Anxiety Are Risk Factors For Getting Irritable Bowel Syndrome, Ola Amer Jasim, Khalid Mahdi Salih

Al-Esraa University College Journal for Medical Sciences

Irritable Bowel Syndrome (IBS) is a functional disease of gastrointestinal tract (GIT) that affects 10-20% of the population worldwide and is distinguished by abdominal pain/discomfort and changes in bowel habits that is accompanied by diarrhea, constipation, or both. Control-based study was conducted on 50 Iraqi patients with IBS who were presented at private clinics for gastrointestinal diseases (GIDs), along with 20 healthy individuals matched in their age and gender to act as control group. The study lasted from November 2021 to May 2022 and aimed to determine the frequency of IBS subtypes among Iraqi patients, as well as the influence …

Complement Component C3 And C4 Levels In Juvenile And Adult Sle Iraqi Patients, Sahar S. Aldhahir, Izzat A. M. Al-Rayahi, Salwa S. Muhsin

Al-Esraa University College Journal for Medical Sciences

Background: Systemic lupus erythematosus (SLE) is an autoimmune disease, with about 15-20% of patients being children. The causes of the disease are a mix of genetic and environmental factors. Complement C3 and C4 play a significant role in the diagnosis and monitoring of disease severity. Additionally, renal function tests are important for monitoring the disease, as the disease affects the kidneys in a large percentage of patients. Aim: The study was primarily conducted to compare complement C3 and C4 levels in Iraqi adult and juvenile patients with systemic lupus erythematosus. Patient and methods: The study consisted of 70 patients, …

Carfilzomib-Loaded Ternary Polypeptide Nanoparticles Stabilized By Polycationic Complexation, Preye Mike Agbana, Ji Eun Park, Piotr G. Rychahou, Kyung-Bo Kim, Younsoo Bae

Markey Cancer Center Faculty Publications

Carfilzomib (CFZ) is a second-generation proteasome inhibitor showing great efficacy in multiple myeloma treatment, yet its clinical applications for other diseases such as solid cancers are limited due to low aqueous solubility and poor biostability. Ternary polypeptide nanoparticles (tPNPs) are drug carriers that we previ- ously reported to overcome these pharmaceutical limitations by entrapping CFZ in the core of the nanopar- ticles and protecting the drugs from degradation in biological media. However, preclinical studies revealed that tPNPs would require further improvement in particle stability to suppress initial burst drug release and thus achieve prolonged inhibition of proteasome activity with CFZ …

Nudcl2 Is Required For Cytokinesis By Stabilizing Rcc2 With Hsp90 At The Midbody, Xiaoyang Xu, Yuliang Huang, Feng Yang, Xiaoxia Sun, Rijin Lin, Jiaxing Feng, Mingyang Yang, Jiaqi Shao, Xiaoqi Liu, Tianhua Zhou, Shanshan Xie, Yuehong Yang

Markey Cancer Center Faculty Publications

Cytokinesis is required for faithful division of cytoplasmic components and duplicated nuclei into two daughter cells. Midbody, a protein-dense organelle that forms at the intercellular bridge, is indispensable for success- ful cytokinesis. However, the regulatory mechanism of cytokinesis at the midbody still remains elusive. Here, we unveil a critical role for NudC-like protein 2 (NudCL2), a co-chaperone of heat shock protein 90 (Hsp90), in cytokinesis regulation by stabilizing regulator of chromosome condensation 2 (RCC2) at the midbody in mam- malian cells. NudCL2 localizes at the midbody, and its downregulation results in cytokinesis failure, multinu- cleation, and midbody disorganization. Using iTRAQ-based …